Restriction map for
pF1KA0078
(Circular) (Six-base) MAPSORT of: pF1KA0078.seq Check: 6502 from: 1 to: 5006
pF1KA0078 5006 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AccI GT'mk_AC
Cuts at: 2044 2044
Size: 5006
Acc65I G'GTAC_C
Cuts at: 2022 2022
Size: 5006
AclI AA'CG_TT
Cuts at: 4846 4846
Size: 5006
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 461 1285 1792 2831 3263 3616 4278 461
Size: 824 507 1039 432 353 662 1189
Fragments arranged by size:
1189 1039 824 662 507 432 353
AflIII A'CryG_T
Cuts at: 650 2358 4158 4439 650
Size: 1708 1800 281 1217
Fragments arranged by size:
1800 1708 1217 281
AlwNI CAG_nnn'CTG
Cuts at: 468 1567 2311 3749 468
Size: 1099 744 1438 1725
Fragments arranged by size:
1725 1438 1099 744
ApaLI G'TGCA_C
Cuts at: 3844 3844
Size: 5006
ApoI r'AATT_y
Cuts at: 155 445 1351 1363 2010 4359 4615 155
Size: 290 906 12 647 2349 256 546
Fragments arranged by size:
2349 906 647 546 290 256 12
AseI AT'TA_AT
Cuts at: 20 20
Size: 5006
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5006
AvaI C'yCGr_G
Cuts at: 2026 2026
Size: 5006
BamHI G'GATC_C
Cuts at: 951 2031 951
Size: 1080 3926
BanI G'GyrC_C
Cuts at: 1167 2022 2436 2684 2719 1167
Size: 855 414 248 35 3454
Fragments arranged by size:
3454 855 414 248 35
BanII G_rGCy'C
Cuts at: 1125 1436 2020 3050 1125
Size: 311 584 1030 3081
Fragments arranged by size:
3081 1030 584 311
BbeI G_GCGC'C
Cuts at: 2440 2688 2440
Size: 248 4758
BbsI GAAGACnn'nnnn_
Cuts at: 1310 1798 1310
Size: 488 4518
BbvCI CC'TCA_GC
Cuts at: 1580 1580
Size: 5006
BciVI GTATCCnnnnn_n'
Cuts at: 2904 3960 5006 2904
Size: 1056 1046 2904
Fragments arranged by size:
2904 1056 1046
BclI T'GATC_A
Cuts at: 459 981 2526 459
Size: 522 1545 2939
Fragments arranged by size:
2939 1545 522
BfrBI ATG'CAT
Cuts at: 1738 1738
Size: 5006
BglII A'GATC_T
Cuts at: 2521 4558 2521
Size: 2037 2969
BlpI GC'TnA_GC
Cuts at: 1238 2118 1238
Size: 880 4126
Bme1580I G_kGCm'C
Cuts at: 2631 2724 3848 2631
Size: 93 1124 3789
Fragments arranged by size:
3789 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 298 2218 2299 2634 4778 298
Size: 1920 81 335 2144 526
Fragments arranged by size:
2144 1920 526 335 81
BmtI G_CTAG'C
Cuts at: 166 166
Size: 5006
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3373 3373
Size: 5006
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1444 1850 1444
Size: 406 4600
Bpu10I CC'TnA_GC
Cuts at: 1580 1580
Size: 5006
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1873 2182 3061 3775 4073 1873
Size: 309 879 714 298 2806
Fragments arranged by size:
2806 879 714 309 298
BsaI GGTCTCn'nnnn_
Cuts at: 4710 4710
Size: 5006
BsaAI yAC'GTr
Cuts at: 2359 2359
Size: 5006
BsaBI GATnn'nnATC
Cuts at: 6 1754 2520 4557 6
Size: 1748 766 2037 455
Fragments arranged by size:
2037 1748 766 455
BsaHI Gr'CG_yC
Cuts at: 2437 2685 3387 4883 2437
Size: 248 702 1496 2560
Fragments arranged by size:
2560 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2195 2716 3394 3805 3952 4226 2195
Size: 521 678 411 147 274 2975
Fragments arranged by size:
2975 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1223 1343 1568 1223
Size: 120 225 4661
Fragments arranged by size:
4661 225 120
BseYI C'CCAG_C
Cuts at: 497 2431 3189 3854 4262 497
Size: 1934 758 665 408 1241
Fragments arranged by size:
1934 1241 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 869 869
Size: 5006
BsiEI CG_ry'CG
Cuts at: 104 2548 2594 3824 104
Size: 2444 46 1230 1286
Fragments arranged by size:
2444 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 703 1818 2020 2798 2988 3848 703
Size: 1115 202 778 190 860 1861
Fragments arranged by size:
1861 1115 860 778 202 190
BsmI GAATG_Cn'
Cuts at: 349 349
Size: 5006
Bsp1286I G_dGCh'C
Cuts at: 703 1125 1436 1818 2020 2631 2724 2798 2988
Size: 422 311 382 202 611 93 74 190
Cuts at: 2988 3050 3848 703
Size: 62 798 1861
Fragments arranged by size:
1861 798 611 422 382 311 202 190
93 74 62
BspHI T'CATG_A
Cuts at: 450 3438 450
Size: 2988 2018
BspMI ACCTGCnnnn'nnnn_
Cuts at: 401 1242 1575 2056 2270 2572 2953 401
Size: 841 333 481 214 302 381 2454
Fragments arranged by size:
2454 841 481 381 333 302 214
BsrBI CCG'CTC
Cuts at: 50 3298 3352 4836 5001 50
Size: 3248 54 1484 165 55
Fragments arranged by size:
3248 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2918 2918
Size: 5006
BsrFI r'CCGG_y
Cuts at: 3004 3185 3004
Size: 181 4825
BssSI C'ACGA_G
Cuts at: 3277 3985 3277
Size: 708 4298
BstAPI GCAn_nnn'nTGC
Cuts at: 2383 2383
Size: 5006
BstBI TT'CG_AA
Cuts at: 300 3367 300
Size: 3067 1939
BstYI r'GATC_y
Cuts at: 1 951 1161 2031 2498 2521 2856 3102 3408
Size: 950 210 870 467 23 335 246 306
Cuts at: 3408 3420 3506 4558 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 950 870 467 449 335 306 246
210 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 398 1526 398
Size: 1128 3878
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1953 2956 3137 3300 1953
Size: 1003 181 163 3659
Fragments arranged by size:
3659 1003 181 163
BtsI GCAGTG_nn'
Cuts at: 466 636 2249 466
Size: 170 1613 3223
Fragments arranged by size:
3223 1613 170
ClaI AT'CG_AT
Cuts at: 488 1427 488
Size: 939 4067
DraI TTT'AAA
Cuts at: 2005 2005
Size: 5006
DrdI GACnn_nn'nnGTC
Cuts at: 2712 4056 2712
Size: 1344 3662
EaeI y'GGCC_r
Cuts at: 149 376 2591 2765 3156 3183 4528 149
Size: 227 2215 174 391 27 1345 627
Fragments arranged by size:
2215 1345 627 391 227 174 27
EagI C'GGCC_G
Cuts at: 2591 2591
Size: 5006
EarI CTCTTCn'nnn_
Cuts at: 524 1063 1439 1712 1718 3029 3239 524
Size: 539 376 273 6 1311 210 2291
Fragments arranged by size:
2291 1311 539 376 273 210 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3214 3956 4102 4816 3214
Size: 742 146 714 3404
Fragments arranged by size:
3404 742 714 146
EcoICRI GAG'CTC
Cuts at: 2018 2018
Size: 5006
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 461 1285 1444 1792 1850 2831 3263 3616 4278
Size: 824 159 348 58 981 432 353 662
Cuts at: 4278 461
Size: 1189
Fragments arranged by size:
1189 981 824 662 432 353 348 159
58
EcoNI CCTnn'n_nnAGG
Cuts at: 397 853 397
Size: 456 4550
EcoO109I rG'GnC_Cy
Cuts at: 142 2145 142
Size: 2003 3003
EcoRI G'AATT_C
Cuts at: 1351 2010 1351
Size: 659 4347
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3025 3025
Size: 5006
FspI TGC'GCA
Cuts at: 2787 2787
Size: 5006
HaeII r_GCGC'y
Cuts at: 2440 2688 3918 4689 2440
Size: 248 1230 771 2757
Fragments arranged by size:
2757 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 901 933 1563 1595 2848 2878 2880 2910 901
Size: 32 630 32 1253 30 2 30 2997
Fragments arranged by size:
2997 1253 630 32 32 30 30 2
HincII GTy'rAC
Cuts at: 390 1086 2045 390
Size: 696 959 3351
Fragments arranged by size:
3351 959 696
KasI G'GCGC_C
Cuts at: 2436 2684 2436
Size: 248 4758
KpnI G_GTAC'C
Cuts at: 2026 2026
Size: 5006
MluI A'CGCG_T
Cuts at: 4439 4439
Size: 5006
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1082 3765 3949 1082
Size: 2683 184 2139
Fragments arranged by size:
2683 2139 184
MscI TGG'CCA
Cuts at: 151 2767 151
Size: 2616 2390
MslI CAynn'nnrTG
Cuts at: 490 2995 3122 490
Size: 2505 127 2374
Fragments arranged by size:
2505 2374 127
MspA1I CmG'CkG
Cuts at: 2117 2431 2791 3575 3820 2117
Size: 314 360 784 245 3303
Fragments arranged by size:
3303 784 360 314 245
NaeI GCC'GGC
Cuts at: 3187 3187
Size: 5006
NarI GG'CG_CC
Cuts at: 2437 2685 2437
Size: 248 4758
NgoMIV G'CCGG_C
Cuts at: 3185 3185
Size: 5006
NheI G'CTAG_C
Cuts at: 162 162
Size: 5006
NsiI A_TGCA'T
Cuts at: 1740 1740
Size: 5006
NspI r_CATG'y
Cuts at: 654 2059 2559 4162 654
Size: 1405 500 1603 1498
Fragments arranged by size:
1603 1498 1405 500
PciI A'CATG_T
Cuts at: 650 4158 650
Size: 3508 1498
PflMI CCAn_nnn'nTGG
Cuts at: 158 158
Size: 5006
PfoI T'CCnGG_A
Cuts at: 1460 1460
Size: 5006
PmeI GTTT'AAAC
Cuts at: 2005 2005
Size: 5006
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3452 3484 3452
Size: 32 4974
PsiI TTA'TAA
Cuts at: 422 422
Size: 5006
PstI C_TGCA'G
Cuts at: 2053 2738 2053
Size: 685 4321
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5006
PvuII CAG'CTG
Cuts at: 2431 2791 2431
Size: 360 4646
RsrII CG'GwC_CG
Cuts at: 3201 3201
Size: 5006
SacI G_AGCT'C
Cuts at: 2020 2020
Size: 5006
SalI G'TCGA_C
Cuts at: 2043 2043
Size: 5006
SapI GCTCTTCn'nnn_
Cuts at: 1439 3029 3239 1439
Size: 1590 210 3206
Fragments arranged by size:
3206 1590 210
SbfI CC_TGCA'GG
Cuts at: 2053 2053
Size: 5006
ScaI AGT'ACT
Cuts at: 4523 4523
Size: 5006
SfcI C'TryA_G
Cuts at: 33 1610 1899 2049 2734 3702 3893 33
Size: 1577 289 150 685 968 191 1146
Fragments arranged by size:
1577 1146 968 685 289 191 150
SfoI GGC'GCC
Cuts at: 2438 2686 2438
Size: 248 4758
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5006
SmaI CCC'GGG
Cuts at: 2028 2028
Size: 5006
SmlI C'TyrA_G
Cuts at: 1852 2161 3076 3790 4052 1852
Size: 309 915 714 262 2806
Fragments arranged by size:
2806 915 714 309 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5006
SphI G_CATG'C
Cuts at: 2059 2559 2059
Size: 500 4506
SspI AAT'ATT
Cuts at: 791 791
Size: 5006
StyI C'CwwG_G
Cuts at: 1984 2140 2494 1984
Size: 156 354 4496
Fragments arranged by size:
4496 354 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 913 2589 2722 3181 3390 913
Size: 1676 133 459 209 2529
Fragments arranged by size:
2529 1676 459 209 133
TatI w'GTAC_w
Cuts at: 4521 4521
Size: 5006
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4523 4555 4523
Size: 32 4974
Tth111I GACn'n_nGTC
Cuts at: 1090 2803 1090
Size: 1713 3293
XbaI T'CTAG_A
Cuts at: 2037 2037
Size: 5006
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2501 2501
Size: 5006
XmaI C'CCGG_G
Cuts at: 2026 2026
Size: 5006
XmnI GAAnn'nnTTC
Cuts at: 446 446
Size: 5006
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NheI NsiI NspI PciI PflMI PfoI PmeI PpiI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BcgI BglI BmgBI
BsaXI BsiWI BsmBI BspEI BsrGI BssHII BstEII BstXI
BstZ17I BtgI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NotI NruI PacI PasI
PmlI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
ZraI