Restriction map for
pF1KA0082
(Circular) (Six-base) MAPSORT of: pF1KA0082.seq Check: 6723 from: 1 to: 5618
pF1KA0082 5618 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 558 558
Size: 5618
AatII G_ACGT'C
Cuts at: 1538 2585 1538
Size: 1047 4571
AccI GT'mk_AC
Cuts at: 1358 2656 1358
Size: 1298 4320
Acc65I G'GTAC_C
Cuts at: 2634 2634
Size: 5618
AclI AA'CG_TT
Cuts at: 5458 5458
Size: 5618
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 390 1474 1800 2008 3443 3875 4622 4890 390
Size: 1084 326 208 1435 432 747 268 1118
Fragments arranged by size:
1435 1118 1084 747 432 326 268 208
AflIII A'CryG_T
Cuts at: 2970 4074 5051 2970
Size: 1104 977 3537
Fragments arranged by size:
3537 1104 977
AgeI A'CCGG_T
Cuts at: 4006 4006
Size: 5618
AhdI GACnn_n'nnGTC
Cuts at: 2568 2568
Size: 5618
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1270 2548 1270
Size: 1278 4340
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1513 1545 1513
Size: 32 5586
AlwNI CAG_nnn'CTG
Cuts at: 522 2923 4490 522
Size: 2401 1567 1650
Fragments arranged by size:
2401 1650 1567
ApaI G_GGCC'C
Cuts at: 1021 1021
Size: 5618
ApaLI G'TGCA_C
Cuts at: 4388 4388
Size: 5618
ApoI r'AATT_y
Cuts at: 599 1146 1579 2144 2161 2622 4971 5227 599
Size: 547 433 565 17 461 2349 256 990
Fragments arranged by size:
2349 990 565 547 461 433 256 17
AseI AT'TA_AT
Cuts at: 20 20
Size: 5618
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5618
AvaI C'yCGr_G
Cuts at: 764 2003 2638 764
Size: 1239 635 3744
Fragments arranged by size:
3744 1239 635
BamHI G'GATC_C
Cuts at: 1727 2643 1727
Size: 916 4702
BanI G'GyrC_C
Cuts at: 2110 2293 2634 3048 3296 3331 2110
Size: 183 341 414 248 35 4397
Fragments arranged by size:
4397 414 341 248 183 35
BanII G_rGCy'C
Cuts at: 554 759 1021 2287 2378 2632 3662 554
Size: 205 262 1266 91 254 1030 2510
Fragments arranged by size:
2510 1266 1030 262 254 205 91
BbeI G_GCGC'C
Cuts at: 3052 3300 3052
Size: 248 5370
BbsI GAAGACnn'nnnn_
Cuts at: 789 1996 789
Size: 1207 4411
BbvCI CC'TCA_GC
Cuts at: 187 1243 187
Size: 1056 4562
BciVI GTATCCnnnnn_n'
Cuts at: 3516 4277 5618 3516
Size: 761 1341 3516
Fragments arranged by size:
3516 1341 761
BclI T'GATC_A
Cuts at: 1562 3138 1562
Size: 1576 4042
BfrBI ATG'CAT
Cuts at: 316 316
Size: 5618
BglI GCCn_nnn'nGGC
Cuts at: 950 2190 950
Size: 1240 4378
BglII A'GATC_T
Cuts at: 1937 3133 5170 1937
Size: 1196 2037 2385
Fragments arranged by size:
2385 2037 1196
BlpI GC'TnA_GC
Cuts at: 911 2305 2730 911
Size: 1394 425 3799
Fragments arranged by size:
3799 1394 425
Bme1580I G_kGCm'C
Cuts at: 1021 1801 3243 3336 4392 1021
Size: 780 1442 93 1056 2247
Fragments arranged by size:
2247 1442 1056 780 93
BmgBI CAC'GTC
Cuts at: 197 197
Size: 5618
BmrI ACTGGGnnnn_n'
Cuts at: 548 2830 2911 3246 5390 548
Size: 2282 81 335 2144 776
Fragments arranged by size:
2282 2144 776 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1149 1408 1700 3985 1149
Size: 259 292 2285 2782
Fragments arranged by size:
2782 2285 292 259
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1057 1576 1873 2271 1057
Size: 519 297 398 4404
Fragments arranged by size:
4404 519 398 297
Bpu10I CC'TnA_GC
Cuts at: 181 187 1243 181
Size: 6 1056 4556
Fragments arranged by size:
4556 1056 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2794 3673 4165 4463 2794
Size: 879 492 298 3949
Fragments arranged by size:
3949 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 268 1565 5322 268
Size: 1297 3757 564
Fragments arranged by size:
3757 1297 564
BsaAI yAC'GTr
Cuts at: 2971 2971
Size: 5618
BsaBI GATnn'nnATC
Cuts at: 6 1810 2272 3132 5169 6
Size: 1804 462 860 2037 455
Fragments arranged by size:
2037 1804 860 462 455
BsaHI Gr'CG_yC
Cuts at: 1535 2582 3049 3297 3999 5495 1535
Size: 1047 467 248 702 1496 1658
Fragments arranged by size:
1658 1496 1047 702 467 248
BsaWI w'CCGG_w
Cuts at: 1292 2807 3328 4006 4280 4427 4838 1292
Size: 1515 521 678 274 147 411 2072
Fragments arranged by size:
2072 1515 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2572 2602 2572
Size: 30 5588
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 129 207 806 129
Size: 78 599 4941
Fragments arranged by size:
4941 599 78
BseYI C'CCAG_C
Cuts at: 3043 3801 4378 4874 3043
Size: 758 577 496 3787
Fragments arranged by size:
3787 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 3160 3206 4414 104
Size: 3056 46 1208 1308
Fragments arranged by size:
3056 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 759 2632 3410 3600 4392 759
Size: 1873 778 190 792 1985
Fragments arranged by size:
1985 1873 792 778 190
BsmI GAATG_Cn'
Cuts at: 136 316 2525 136
Size: 180 2209 3229
Fragments arranged by size:
3229 2209 180
BsmBI CGTCTCn'nnnn_
Cuts at: 381 381
Size: 5618
Bsp1286I G_dGCh'C
Cuts at: 554 759 1021 1801 2287 2378 2632 3243 3336
Size: 205 262 780 486 91 254 611 93
Cuts at: 3336 3410 3600 3662 4392 554
Size: 74 190 62 730 1780
Fragments arranged by size:
1780 780 730 611 486 262 254 205
190 93 91 74 62
BspEI T'CCGG_A
Cuts at: 1292 4838 1292
Size: 3546 2072
BspHI T'CATG_A
Cuts at: 4794 4794
Size: 5618
BspMI ACCTGCnnnn'nnnn_
Cuts at: 558 1238 1370 2668 2882 3184 3565 558
Size: 680 132 1298 214 302 381 2611
Fragments arranged by size:
2611 1298 680 381 302 214 132
BsrBI CCG'CTC
Cuts at: 50 3910 3964 5448 5613 50
Size: 3860 54 1484 165 55
Fragments arranged by size:
3860 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 824 2468 3530 824
Size: 1644 1062 2912
Fragments arranged by size:
2912 1644 1062
BsrFI r'CCGG_y
Cuts at: 2327 2498 3616 3797 4006 2327
Size: 171 1118 181 209 3939
Fragments arranged by size:
3939 1118 209 181 171
BsrGI T'GTAC_A
Cuts at: 2225 2225
Size: 5618
BssSI C'ACGA_G
Cuts at: 1742 3889 4247 1742
Size: 2147 358 3113
Fragments arranged by size:
3113 2147 358
BstAPI GCAn_nnn'nTGC
Cuts at: 175 2995 175
Size: 2820 2798
BstBI TT'CG_AA
Cuts at: 1070 3979 1070
Size: 2909 2709
BstYI r'GATC_y
Cuts at: 1 1238 1727 1859 1937 2643 3110 3133 3468
Size: 1237 489 132 78 706 467 23 335
Cuts at: 3468 3714 4726 4812 4824 5170 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1237 1012 706 489 467 449 346 335
246 132 86 78 23 12
BtgI C'CryG_G
Cuts at: 868 2342 868
Size: 1474 4144
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3568 3749 3912 3568
Size: 181 163 5274
Fragments arranged by size:
5274 181 163
BtsI GCAGTG_nn'
Cuts at: 134 715 1499 1831 1905 2861 134
Size: 581 784 332 74 956 2891
Fragments arranged by size:
2891 956 784 581 332 74
DraI TTT'AAA
Cuts at: 2617 2617
Size: 5618
DraIII CAC_nnn'GTG
Cuts at: 702 1678 702
Size: 976 4642
DrdI GACnn_nn'nnGTC
Cuts at: 3324 4182 3324
Size: 858 4760
EaeI y'GGCC_r
Cuts at: 392 1953 2496 3203 3377 3768 3795 5140 392
Size: 1561 543 707 174 391 27 1345 870
Fragments arranged by size:
1561 1345 870 707 543 391 174 27
EagI C'GGCC_G
Cuts at: 3203 3203
Size: 5618
EarI CTCTTCn'nnn_
Cuts at: 106 477 680 737 1072 1399 1492 2231 2509
Size: 371 203 57 335 327 93 739 278
Cuts at: 2509 3641 3851 106
Size: 1132 210 1873
Fragments arranged by size:
1873 1132 739 371 335 327 278 210
203 93 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1852 3826 4136 4282 5428 1852
Size: 1974 310 146 1146 2042
Fragments arranged by size:
2042 1974 1146 310 146
EcoICRI GAG'CTC
Cuts at: 757 2630 757
Size: 1873 3745
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 390 1057 1474 1576 1800 1873 2008 2271 3443
Size: 667 417 102 224 73 135 263 1172
Cuts at: 3443 3875 4622 4890 390
Size: 432 747 268 1118
Fragments arranged by size:
1172 1118 747 667 432 417 268 263
224 135 102 73
EcoO109I rG'GnC_Cy
Cuts at: 1017 1018 1449 2757 1017
Size: 1 431 1308 3878
Fragments arranged by size:
3878 1308 431 1
EcoRI G'AATT_C
Cuts at: 2144 2622 2144
Size: 478 5140
EcoRV GAT'ATC
Cuts at: 1113 1113
Size: 5618
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 420 709 931 2433 3637 420
Size: 289 222 1502 1204 2401
Fragments arranged by size:
2401 1502 1204 289 222
FspI TGC'GCA
Cuts at: 939 3399 939
Size: 2460 3158
HaeII r_GCGC'y
Cuts at: 1282 3052 3300 4322 5301 1282
Size: 1770 248 1022 979 1599
Fragments arranged by size:
1770 1599 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 556 588 1033 1065 1620 1652 1756 1788 2554
Size: 32 445 32 555 32 104 32 766
Cuts at: 2554 2572 2586 2604 3460 3490 3492 3522 556
Size: 18 14 18 856 30 2 30 2652
Fragments arranged by size:
2652 856 766 555 445 104 32 32
32 32 30 30 18 18 14 2
HincII GTy'rAC
Cuts at: 1539 2657 1539
Size: 1118 4500
HindIII A'AGCT_T
Cuts at: 385 2400 385
Size: 2015 3603
KasI G'GCGC_C
Cuts at: 3048 3296 3048
Size: 248 5370
KpnI G_GTAC'C
Cuts at: 2638 2638
Size: 5618
MluI A'CGCG_T
Cuts at: 5051 5051
Size: 5618
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 424 1556 1740 1787 2243 4289 4473 424
Size: 1132 184 47 456 2046 184 1569
Fragments arranged by size:
2046 1569 1132 456 184 184 47
MscI TGG'CCA
Cuts at: 394 3379 394
Size: 2985 2633
MslI CAynn'nnrTG
Cuts at: 2212 2530 2599 3607 3734 2212
Size: 318 69 1008 127 4096
Fragments arranged by size:
4096 1008 318 127 69
MspA1I CmG'CkG
Cuts at: 870 1254 1515 1883 1970 2729 3043 3403 4416
Size: 384 261 368 87 759 314 360 1013
Cuts at: 4416 4661 870
Size: 245 1827
Fragments arranged by size:
1827 1013 759 384 368 360 314 261
245 87
NaeI GCC'GGC
Cuts at: 2500 3799 2500
Size: 1299 4319
NarI GG'CG_CC
Cuts at: 3049 3297 3049
Size: 248 5370
NcoI C'CATG_G
Cuts at: 2342 2342
Size: 5618
NgoMIV G'CCGG_C
Cuts at: 2498 3797 2498
Size: 1299 4319
NsiI A_TGCA'T
Cuts at: 318 318
Size: 5618
NspI r_CATG'y
Cuts at: 858 991 2671 3171 4078 858
Size: 133 1680 500 907 2398
Fragments arranged by size:
2398 1680 907 500 133
PciI A'CATG_T
Cuts at: 4074 4074
Size: 5618
PfoI T'CCnGG_A
Cuts at: 1154 1154
Size: 5618
PmeI GTTT'AAAC
Cuts at: 2617 2617
Size: 5618
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 336 368 1894 1926 4757 4789 336
Size: 32 1526 32 2831 32 1165
Fragments arranged by size:
2831 1526 1165 32 32 32
PspOMI G'GGCC_C
Cuts at: 1017 1017
Size: 5618
PstI C_TGCA'G
Cuts at: 1235 1628 2463 2665 3350 1235
Size: 393 835 202 685 3503
Fragments arranged by size:
3503 835 685 393 202
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5618
PvuII CAG'CTG
Cuts at: 1254 1515 3043 3403 1254
Size: 261 1528 360 3469
Fragments arranged by size:
3469 1528 360 261
RsrII CG'GwC_CG
Cuts at: 3813 3813
Size: 5618
SacI G_AGCT'C
Cuts at: 759 2632 759
Size: 1873 3745
SacII CC_GC'GG
Cuts at: 871 871
Size: 5618
SalI G'TCGA_C
Cuts at: 2655 2655
Size: 5618
SapI GCTCTTCn'nnn_
Cuts at: 680 2509 3641 3851 680
Size: 1829 1132 210 2447
Fragments arranged by size:
2447 1829 1132 210
SbfI CC_TGCA'GG
Cuts at: 1235 2665 1235
Size: 1430 4188
ScaI AGT'ACT
Cuts at: 5135 5135
Size: 5618
SfcI C'TryA_G
Cuts at: 33 1231 1617 1624 2459 2661 3346 4339 4530
Size: 1198 386 7 835 202 685 993 191
Cuts at: 4530 33
Size: 1121
Fragments arranged by size:
1198 1121 993 835 685 386 202 191
7
SfoI GGC'GCC
Cuts at: 3050 3298 3050
Size: 248 5370
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5618
SmaI CCC'GGG
Cuts at: 2005 2640 2005
Size: 635 4983
SmlI C'TyrA_G
Cuts at: 2773 3688 4180 4442 2773
Size: 915 492 262 3949
Fragments arranged by size:
3949 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5618
SphI G_CATG'C
Cuts at: 991 2671 3171 991
Size: 1680 500 3438
Fragments arranged by size:
3438 1680 500
SspI AAT'ATT
Cuts at: 1500 1500
Size: 5618
StyI C'CwwG_G
Cuts at: 2342 2435 2573 2752 3106 2342
Size: 93 138 179 354 4854
Fragments arranged by size:
4854 354 179 138 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 292 3201 3334 3793 4002 292
Size: 2909 133 459 209 1908
Fragments arranged by size:
2909 1908 459 209 133
TatI w'GTAC_w
Cuts at: 920 1367 2225 5133 920
Size: 447 858 2908 1405
Fragments arranged by size:
2908 1405 858 447
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 585 617 2058 2090 5135 5167 585
Size: 32 1441 32 3045 32 1036
Fragments arranged by size:
3045 1441 1036 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3415 3415
Size: 5618
XbaI T'CTAG_A
Cuts at: 2649 2649
Size: 5618
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3113 3113
Size: 5618
XmaI C'CCGG_G
Cuts at: 2003 2638 2003
Size: 635 4983
XmnI GAAnn'nnTTC
Cuts at: 348 2413 2416 348
Size: 2065 3 3550
Fragments arranged by size:
3550 2065 3
ZraI GAC'GTC
Cuts at: 1536 2583 1536
Size: 1047 4571
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PfoI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AleI AscI AvrII BaeI BcgI BmtI
BsgI BsiWI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI EcoNI FseI FspAI HpaI MfeI NdeI NheI
NotI NruI PacI PasI PflMI PmlI PpuMI PshAI
PsiI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI