Restriction map for
pF1KA0089
(Circular) (Six-base) MAPSORT of: pF1KA0089.seq Check: 233 from: 1 to: 4166
pF1KA0089 4166 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AccI GT'mk_AC
Cuts at: 856 1204 856
Size: 348 3818
Acc65I G'GTAC_C
Cuts at: 1182 1182
Size: 4166
AclI AA'CG_TT
Cuts at: 4006 4006
Size: 4166
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 1046 1123 1991 2423 3170 3438 184
Size: 862 77 868 432 747 268 912
Fragments arranged by size:
912 868 862 747 432 268 77
AfeI AGC'GCT
Cuts at: 452 452
Size: 4166
AflII C'TTAA_G
Cuts at: 721 721
Size: 4166
AflIII A'CryG_T
Cuts at: 1518 2622 3599 1518
Size: 1104 977 2085
Fragments arranged by size:
2085 1104 977
AgeI A'CCGG_T
Cuts at: 2554 2554
Size: 4166
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1037 1037
Size: 4166
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 141 173 141
Size: 32 4134
AlwNI CAG_nnn'CTG
Cuts at: 416 700 1471 3038 416
Size: 284 771 1567 1544
Fragments arranged by size:
1567 1544 771 284
ApaI G_GGCC'C
Cuts at: 490 490
Size: 4166
ApaLI G'TGCA_C
Cuts at: 2936 2936
Size: 4166
ApoI r'AATT_y
Cuts at: 205 666 1170 3519 3775 205
Size: 461 504 2349 256 596
Fragments arranged by size:
2349 596 504 461 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4166
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4166
AvaI C'yCGr_G
Cuts at: 144 490 1186 144
Size: 346 696 3124
Fragments arranged by size:
3124 696 346
BamHI G'GATC_C
Cuts at: 1191 1191
Size: 4166
BanI G'GyrC_C
Cuts at: 117 1182 1596 1844 1879 117
Size: 1065 414 248 35 2404
Fragments arranged by size:
2404 1065 414 248 35
BanII G_rGCy'C
Cuts at: 145 490 1180 2210 145
Size: 345 690 1030 2101
Fragments arranged by size:
2101 1030 690 345
BbeI G_GCGC'C
Cuts at: 121 1600 1848 121
Size: 1479 248 2439
Fragments arranged by size:
2439 1479 248
BbsI GAAGACnn'nnnn_
Cuts at: 964 1129 964
Size: 165 4001
BciVI GTATCCnnnnn_n'
Cuts at: 2064 2825 4166 2064
Size: 761 1341 2064
Fragments arranged by size:
2064 1341 761
BclI T'GATC_A
Cuts at: 896 1686 896
Size: 790 3376
BglII A'GATC_T
Cuts at: 1094 1681 3718 1094
Size: 587 2037 1542
Fragments arranged by size:
2037 1542 587
BlpI GC'TnA_GC
Cuts at: 1278 1278
Size: 4166
Bme1580I G_kGCm'C
Cuts at: 443 490 1791 1884 2940 443
Size: 47 1301 93 1056 1669
Fragments arranged by size:
1669 1301 1056 93 47
BmrI ACTGGGnnnn_n'
Cuts at: 162 440 962 1378 1459 1794 3938 162
Size: 278 522 416 81 335 2144 390
Fragments arranged by size:
2144 522 416 390 335 278 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2533 2533
Size: 4166
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1342 2221 2713 3011 1342
Size: 879 492 298 2497
Fragments arranged by size:
2497 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 595 3870 595
Size: 3275 891
BsaAI yAC'GTr
Cuts at: 1519 1519
Size: 4166
BsaBI GATnn'nnATC
Cuts at: 6 1680 3717 6
Size: 1674 2037 455
Fragments arranged by size:
2037 1674 455
BsaHI Gr'CG_yC
Cuts at: 118 1597 1845 2547 4043 118
Size: 1479 248 702 1496 241
Fragments arranged by size:
1496 1479 702 248 241
BsaWI w'CCGG_w
Cuts at: 1355 1876 2554 2828 2975 3386 1355
Size: 521 678 274 147 411 2135
Fragments arranged by size:
2135 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 453 745 1591 2349 2926 3422 453
Size: 292 846 758 577 496 1197
Fragments arranged by size:
1197 846 758 577 496 292
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 382
Size: 4166
BsiEI CG_ry'CG
Cuts at: 104 791 1708 1754 2962 104
Size: 687 917 46 1208 1308
Fragments arranged by size:
1308 1208 917 687 46
BsiHKAI G_wGCw'C
Cuts at: 1180 1958 2148 2940 1180
Size: 778 190 792 2406
Fragments arranged by size:
2406 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 145 443 490 1180 1791 1884 1958 2148 2210
Size: 298 47 690 611 93 74 190 62
Cuts at: 2210 2940 145
Size: 730 1371
Fragments arranged by size:
1371 730 690 611 298 190 93 74
62 47
BspEI T'CCGG_A
Cuts at: 3386 3386
Size: 4166
BspHI T'CATG_A
Cuts at: 3342 3342
Size: 4166
BspMI ACCTGCnnnn'nnnn_
Cuts at: 383 1216 1430 1732 2113 383
Size: 833 214 302 381 2436
Fragments arranged by size:
2436 833 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2458 2512 3996 4161 50
Size: 2408 54 1484 165 55
Fragments arranged by size:
2408 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 566 2078 566
Size: 1512 2654
BsrFI r'CCGG_y
Cuts at: 2164 2345 2554 2164
Size: 181 209 3776
Fragments arranged by size:
3776 209 181
BssSI C'ACGA_G
Cuts at: 2437 2795 2437
Size: 358 3808
BstAPI GCAn_nnn'nTGC
Cuts at: 1543 1543
Size: 4166
BstBI TT'CG_AA
Cuts at: 672 2527 672
Size: 1855 2311
BstXI CCAn_nnnn'nTGG
Cuts at: 579 579
Size: 4166
BstYI r'GATC_y
Cuts at: 1 833 1094 1191 1658 1681 2016 2262 3274
Size: 832 261 97 467 23 335 246 1012
Cuts at: 3274 3360 3372 3718 1
Size: 86 12 346 449
Fragments arranged by size:
1012 832 467 449 346 335 261 246
97 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4166
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2116 2297 2460 2116
Size: 181 163 3822
Fragments arranged by size:
3822 181 163
BtsI GCAGTG_nn'
Cuts at: 1091 1409 1091
Size: 318 3848
DraI TTT'AAA
Cuts at: 1165 1165
Size: 4166
DrdI GACnn_nn'nnGTC
Cuts at: 1872 2730 1872
Size: 858 3308
EaeI y'GGCC_r
Cuts at: 788 887 932 1751 1925 2316 2343 3688 788
Size: 99 45 819 174 391 27 1345 1266
Fragments arranged by size:
1345 1266 819 391 174 99 45 27
EagI C'GGCC_G
Cuts at: 788 1751 788
Size: 963 3203
EarI CTCTTCn'nnn_
Cuts at: 646 962 2189 2399 646
Size: 316 1227 210 2413
Fragments arranged by size:
2413 1227 316 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 787 933 2374 2684 2830 3976 787
Size: 146 1441 310 146 1146 977
Fragments arranged by size:
1441 1146 977 310 146 146
EcoICRI GAG'CTC
Cuts at: 1178 1178
Size: 4166
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 1046 1123 1991 2423 3170 3438 184
Size: 862 77 868 432 747 268 912
Fragments arranged by size:
912 868 862 747 432 268 77
EcoNI CCTnn'n_nnAGG
Cuts at: 469 469
Size: 4166
EcoO109I rG'GnC_Cy
Cuts at: 486 487 1305 486
Size: 1 818 3347
Fragments arranged by size:
3347 818 1
EcoRI G'AATT_C
Cuts at: 1170 1170
Size: 4166
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2185 2185
Size: 4166
FspI TGC'GCA
Cuts at: 1947 1947
Size: 4166
HaeII r_GCGC'y
Cuts at: 121 454 1600 1848 2870 3849 121
Size: 333 1146 248 1022 979 438
Fragments arranged by size:
1146 1022 979 438 333 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1114 1146 2008 2038 2040 2070 1114
Size: 32 862 30 2 30 3210
Fragments arranged by size:
3210 862 32 30 30 2
HincII GTy'rAC
Cuts at: 1205 1205
Size: 4166
KasI G'GCGC_C
Cuts at: 117 1596 1844 117
Size: 1479 248 2439
Fragments arranged by size:
2439 1479 248
KpnI G_GTAC'C
Cuts at: 1186 1186
Size: 4166
MluI A'CGCG_T
Cuts at: 3599 3599
Size: 4166
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 953 2837 3021 953
Size: 1884 184 2098
Fragments arranged by size:
2098 1884 184
MscI TGG'CCA
Cuts at: 1927 1927
Size: 4166
MslI CAynn'nnrTG
Cuts at: 290 547 814 2155 2282 290
Size: 257 267 1341 127 2174
Fragments arranged by size:
2174 1341 267 257 127
MspA1I CmG'CkG
Cuts at: 115 163 767 1277 1591 1951 2964 3209 115
Size: 48 604 510 314 360 1013 245 1072
Fragments arranged by size:
1072 1013 604 510 360 314 245 48
NaeI GCC'GGC
Cuts at: 2347 2347
Size: 4166
NarI GG'CG_CC
Cuts at: 118 1597 1845 118
Size: 1479 248 2439
Fragments arranged by size:
2439 1479 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4166
NgoMIV G'CCGG_C
Cuts at: 2345 2345
Size: 4166
NspI r_CATG'y
Cuts at: 1219 1719 2626 1219
Size: 500 907 2759
Fragments arranged by size:
2759 907 500
PciI A'CATG_T
Cuts at: 2622 2622
Size: 4166
PfoI T'CCnGG_A
Cuts at: 306 306
Size: 4166
PmeI GTTT'AAAC
Cuts at: 1165 1165
Size: 4166
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3305 3337 3305
Size: 32 4134
PspOMI G'GGCC_C
Cuts at: 486 486
Size: 4166
PstI C_TGCA'G
Cuts at: 588 659 1086 1213 1898 588
Size: 71 427 127 685 2856
Fragments arranged by size:
2856 685 427 127 71
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4166
PvuII CAG'CTG
Cuts at: 163 1591 1951 163
Size: 1428 360 2378
Fragments arranged by size:
2378 1428 360
RsrII CG'GwC_CG
Cuts at: 2361 2361
Size: 4166
SacI G_AGCT'C
Cuts at: 1180 1180
Size: 4166
SalI G'TCGA_C
Cuts at: 1203 1203
Size: 4166
SapI GCTCTTCn'nnn_
Cuts at: 2189 2399 2189
Size: 210 3956
SbfI CC_TGCA'GG
Cuts at: 1213 1213
Size: 4166
ScaI AGT'ACT
Cuts at: 3683 3683
Size: 4166
SfcI C'TryA_G
Cuts at: 33 584 655 857 1082 1209 1894 2887 3078
Size: 551 71 202 225 127 685 993 191
Cuts at: 3078 33
Size: 1121
Fragments arranged by size:
1121 993 685 551 225 202 191 127
71
SfoI GGC'GCC
Cuts at: 119 1598 1846 119
Size: 1479 248 2439
Fragments arranged by size:
2439 1479 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4166
SmaI CCC'GGG
Cuts at: 1188 1188
Size: 4166
SmlI C'TyrA_G
Cuts at: 721 1321 2236 2728 2990 721
Size: 600 915 492 262 1897
Fragments arranged by size:
1897 915 600 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4166
SphI G_CATG'C
Cuts at: 1219 1719 1219
Size: 500 3666
StuI AGG'CCT
Cuts at: 940 940
Size: 4166
StyI C'CwwG_G
Cuts at: 107 1005 1300 1654 107
Size: 898 295 354 2619
Fragments arranged by size:
2619 898 354 295
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1749 1882 2341 2550 1749
Size: 133 459 209 3365
Fragments arranged by size:
3365 459 209 133
TatI w'GTAC_w
Cuts at: 3681 3681
Size: 4166
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3683 3715 3683
Size: 32 4134
Tth111I GACn'n_nGTC
Cuts at: 1963 1963
Size: 4166
XbaI T'CTAG_A
Cuts at: 1197 1197
Size: 4166
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 450 969 1661 450
Size: 519 692 2955
Fragments arranged by size:
2955 692 519
XmaI C'CCGG_G
Cuts at: 1186 1186
Size: 4166
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AhdI AleI AscI AvrII BaeI BbvCI
BcgI BfrBI BglI BmgBI BmtI BpmI Bpu10I BsaXI
BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI