Restriction map for
pF1KA0092
(Circular) (Six-base) MAPSORT of: pF1KA0092.seq Check: 109 from: 1 to: 4535
pF1KA0092 4535 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AccI GT'mk_AC
Cuts at: 1573 1573
Size: 4535
Acc65I G'GTAC_C
Cuts at: 1551 1551
Size: 4535
AclI AA'CG_TT
Cuts at: 4375 4375
Size: 4535
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1447 2360 2792 3539 3807 1447
Size: 913 432 747 268 2175
Fragments arranged by size:
2175 913 747 432 268
AflII C'TTAA_G
Cuts at: 325 325
Size: 4535
AflIII A'CryG_T
Cuts at: 648 1887 2991 3968 648
Size: 1239 1104 977 1215
Fragments arranged by size:
1239 1215 1104 977
AgeI A'CCGG_T
Cuts at: 2923 2923
Size: 4535
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 737 737
Size: 4535
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 176 208 176
Size: 32 4503
AlwNI CAG_nnn'CTG
Cuts at: 1840 3407 1840
Size: 1567 2968
ApaLI G'TGCA_C
Cuts at: 3305 3305
Size: 4535
ApoI r'AATT_y
Cuts at: 456 1138 1494 1539 3888 4144 456
Size: 682 356 45 2349 256 847
Fragments arranged by size:
2349 847 682 356 256 45
AseI AT'TA_AT
Cuts at: 20 1097 20
Size: 1077 3458
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4535
AvaI C'yCGr_G
Cuts at: 1555 1555
Size: 4535
BamHI G'GATC_C
Cuts at: 1560 1560
Size: 4535
BanI G'GyrC_C
Cuts at: 1551 1965 2213 2248 1551
Size: 414 248 35 3838
Fragments arranged by size:
3838 414 248 35
BanII G_rGCy'C
Cuts at: 1549 2579 1549
Size: 1030 3505
BbeI G_GCGC'C
Cuts at: 1969 2217 1969
Size: 248 4287
BciVI GTATCCnnnnn_n'
Cuts at: 2433 3194 4535 2433
Size: 761 1341 2433
Fragments arranged by size:
2433 1341 761
BclI T'GATC_A
Cuts at: 639 1158 2055 639
Size: 519 897 3119
Fragments arranged by size:
3119 897 519
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4535
BglII A'GATC_T
Cuts at: 2050 4087 2050
Size: 2037 2498
BlpI GC'TnA_GC
Cuts at: 164 1647 164
Size: 1483 3052
Bme1580I G_kGCm'C
Cuts at: 2160 2253 3309 2160
Size: 93 1056 3386
Fragments arranged by size:
3386 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1747 1828 2163 4307 1747
Size: 81 335 2144 1975
Fragments arranged by size:
2144 1975 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2902 2902
Size: 4535
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1324 1460 1324
Size: 136 4399
Bpu10I CC'TnA_GC
Cuts at: 794 794
Size: 4535
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 1711 2590 3082 3380 382
Size: 1329 879 492 298 1537
Fragments arranged by size:
1537 1329 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1428 4239 1428
Size: 2811 1724
BsaAI yAC'GTr
Cuts at: 1888 1888
Size: 4535
BsaBI GATnn'nnATC
Cuts at: 6 2049 4086 6
Size: 2043 2037 455
Fragments arranged by size:
2043 2037 455
BsaHI Gr'CG_yC
Cuts at: 118 1966 2214 2916 4412 118
Size: 1848 248 702 1496 241
Fragments arranged by size:
1848 1496 702 248 241
BsaWI w'CCGG_w
Cuts at: 1724 2245 2923 3197 3344 3755 1724
Size: 521 678 274 147 411 2504
Fragments arranged by size:
2504 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1074 1117 1074
Size: 43 4492
BseYI C'CCAG_C
Cuts at: 1298 1960 2718 3295 3791 1298
Size: 662 758 577 496 2042
Fragments arranged by size:
2042 758 662 577 496
BsiEI CG_ry'CG
Cuts at: 104 2077 2123 3331 104
Size: 1973 46 1208 1308
Fragments arranged by size:
1973 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1549 2327 2517 3309 1549
Size: 778 190 792 2775
Fragments arranged by size:
2775 792 778 190
BsmI GAATG_Cn'
Cuts at: 198 198
Size: 4535
Bsp1286I G_dGCh'C
Cuts at: 1549 2160 2253 2327 2517 2579 3309 1549
Size: 611 93 74 190 62 730 2775
Fragments arranged by size:
2775 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3755 3755
Size: 4535
BspHI T'CATG_A
Cuts at: 3711 3711
Size: 4535
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1585 1799 2101 2482 1585
Size: 214 302 381 3638
Fragments arranged by size:
3638 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2827 2881 4365 4530 50
Size: 2777 54 1484 165 55
Fragments arranged by size:
2777 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1002 2447 1002
Size: 1445 3090
BsrFI r'CCGG_y
Cuts at: 2533 2714 2923 2533
Size: 181 209 4145
Fragments arranged by size:
4145 209 181
BssSI C'ACGA_G
Cuts at: 1043 2806 3164 1043
Size: 1763 358 2414
Fragments arranged by size:
2414 1763 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1912 1912
Size: 4535
BstBI TT'CG_AA
Cuts at: 1284 2896 1284
Size: 1612 2923
BstYI r'GATC_y
Cuts at: 1 675 1560 2027 2050 2385 2631 3643 3729
Size: 674 885 467 23 335 246 1012 86
Cuts at: 3729 3741 4087 1
Size: 12 346 449
Fragments arranged by size:
1012 885 674 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 935 107
Size: 828 3707
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2485 2666 2829 2485
Size: 181 163 4191
Fragments arranged by size:
4191 181 163
BtsI GCAGTG_nn'
Cuts at: 1778 1778
Size: 4535
DraI TTT'AAA
Cuts at: 1534 1534
Size: 4535
DraIII CAC_nnn'GTG
Cuts at: 1048 1048
Size: 4535
DrdI GACnn_nn'nnGTC
Cuts at: 2241 3099 2241
Size: 858 3677
EaeI y'GGCC_r
Cuts at: 2120 2294 2685 2712 4057 2120
Size: 174 391 27 1345 2598
Fragments arranged by size:
2598 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2120 2120
Size: 4535
EarI CTCTTCn'nnn_
Cuts at: 2558 2768 2558
Size: 210 4325
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2743 3053 3199 4345 2743
Size: 310 146 1146 2933
Fragments arranged by size:
2933 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1547 1547
Size: 4535
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1324 1447 1460 2360 2792 3539 3807 1324
Size: 123 13 900 432 747 268 2052
Fragments arranged by size:
2052 900 747 432 268 123 13
EcoO109I rG'GnC_Cy
Cuts at: 717 1674 717
Size: 957 3578
EcoRI G'AATT_C
Cuts at: 456 1494 1539 456
Size: 1038 45 3452
Fragments arranged by size:
3452 1038 45
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2554 2554
Size: 4535
FspI TGC'GCA
Cuts at: 2316 2316
Size: 4535
HaeII r_GCGC'y
Cuts at: 1969 2217 3239 4218 1969
Size: 248 1022 979 2286
Fragments arranged by size:
2286 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 577 609 1093 1125 1143 1175 1443 1475 2377
Size: 32 484 32 18 32 268 32 902
Cuts at: 2377 2407 2409 2439 577
Size: 30 2 30 2673
Fragments arranged by size:
2673 902 484 268 32 32 32 32
30 30 18 2
HincII GTy'rAC
Cuts at: 1574 1574
Size: 4535
HindIII A'AGCT_T
Cuts at: 986 986
Size: 4535
KasI G'GCGC_C
Cuts at: 1965 2213 1965
Size: 248 4287
KpnI G_GTAC'C
Cuts at: 1555 1555
Size: 4535
MfeI C'AATT_G
Cuts at: 410 544 410
Size: 134 4401
MluI A'CGCG_T
Cuts at: 3968 3968
Size: 4535
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 725 1112 1199 3206 3390 725
Size: 387 87 2007 184 1870
Fragments arranged by size:
2007 1870 387 184 87
MscI TGG'CCA
Cuts at: 2296 2296
Size: 4535
MslI CAynn'nnrTG
Cuts at: 2524 2651 2524
Size: 127 4408
MspA1I CmG'CkG
Cuts at: 802 1302 1646 1960 2320 3333 3578 802
Size: 500 344 314 360 1013 245 1759
Fragments arranged by size:
1759 1013 500 360 344 314 245
NaeI GCC'GGC
Cuts at: 2716 2716
Size: 4535
NarI GG'CG_CC
Cuts at: 1966 2214 1966
Size: 248 4287
NcoI C'CATG_G
Cuts at: 107 107
Size: 4535
NdeI CA'TA_TG
Cuts at: 213 213
Size: 4535
NgoMIV G'CCGG_C
Cuts at: 2714 2714
Size: 4535
NspI r_CATG'y
Cuts at: 558 577 652 789 1482 1588 2088 2995 558
Size: 19 75 137 693 106 500 907 2098
Fragments arranged by size:
2098 907 693 500 137 106 75 19
PciI A'CATG_T
Cuts at: 648 2991 648
Size: 2343 2192
PflMI CCAn_nnn'nTGG
Cuts at: 936 936
Size: 4535
PfoI T'CCnGG_A
Cuts at: 1184 1184
Size: 4535
PmeI GTTT'AAAC
Cuts at: 1534 1534
Size: 4535
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3674 3706 3674
Size: 32 4503
PshAI GACnn'nnGTC
Cuts at: 595 595
Size: 4535
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 932 964 932
Size: 32 4503
PstI C_TGCA'G
Cuts at: 516 1582 2267 516
Size: 1066 685 2784
Fragments arranged by size:
2784 1066 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4535
PvuII CAG'CTG
Cuts at: 802 1302 1960 2320 802
Size: 500 658 360 3017
Fragments arranged by size:
3017 658 500 360
RsrII CG'GwC_CG
Cuts at: 2730 2730
Size: 4535
SacI G_AGCT'C
Cuts at: 1549 1549
Size: 4535
SalI G'TCGA_C
Cuts at: 1572 1572
Size: 4535
SapI GCTCTTCn'nnn_
Cuts at: 2558 2768 2558
Size: 210 4325
SbfI CC_TGCA'GG
Cuts at: 1582 1582
Size: 4535
ScaI AGT'ACT
Cuts at: 428 4052 428
Size: 3624 911
SfcI C'TryA_G
Cuts at: 33 512 1578 2263 3256 3447 33
Size: 479 1066 685 993 191 1121
Fragments arranged by size:
1121 1066 993 685 479 191
SfoI GGC'GCC
Cuts at: 1967 2215 1967
Size: 248 4287
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4535
SmaI CCC'GGG
Cuts at: 1557 1557
Size: 4535
SmlI C'TyrA_G
Cuts at: 325 361 1690 2605 3097 3359 325
Size: 36 1329 915 492 262 1501
Fragments arranged by size:
1501 1329 915 492 262 36
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4535
SphI G_CATG'C
Cuts at: 577 789 1588 2088 577
Size: 212 799 500 3024
Fragments arranged by size:
3024 799 500 212
StyI C'CwwG_G
Cuts at: 107 1669 2023 107
Size: 1562 354 2619
Fragments arranged by size:
2619 1562 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2118 2251 2710 2919 2118
Size: 133 459 209 3734
Fragments arranged by size:
3734 459 209 133
TatI w'GTAC_w
Cuts at: 426 4050 426
Size: 3624 911
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4052 4084 4052
Size: 32 4503
Tth111I GACn'n_nGTC
Cuts at: 2332 2332
Size: 4535
XbaI T'CTAG_A
Cuts at: 400 819 1566 400
Size: 419 747 3369
Fragments arranged by size:
3369 747 419
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2030 2030
Size: 4535
XmaI C'CCGG_G
Cuts at: 1555 1555
Size: 4535
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PshAI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI BsaXI
BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HpaI
NheI NotI NruI NsiI PacI PasI PmlI PpuMI
PsiI PspOMI PspXI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI