Restriction map for
pF1KA0095
(Circular) (Six-base) MAPSORT of: pF1KA0095.seq Check: 6525 from: 1 to: 5570
pF1KA0095 5570 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 716 716
Size: 5570
AatII G_ACGT'C
Cuts at: 1304 1304
Size: 5570
AccI GT'mk_AC
Cuts at: 2608 2608
Size: 5570
Acc65I G'GTAC_C
Cuts at: 2586 2586
Size: 5570
AclI AA'CG_TT
Cuts at: 5410 5410
Size: 5570
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 121 433 853 1375 1624 2116 3395 3827 4574
Size: 312 420 522 249 492 1279 432 747
Cuts at: 4574 4842 121
Size: 268 849
Fragments arranged by size:
1279 849 747 522 492 432 420 312
268 249
AfeI AGC'GCT
Cuts at: 2254 2254
Size: 5570
AflIII A'CryG_T
Cuts at: 773 803 1799 2922 4026 5003 773
Size: 30 996 1123 1104 977 1340
Fragments arranged by size:
1340 1123 1104 996 977 30
AgeI A'CCGG_T
Cuts at: 3958 3958
Size: 5570
AleI CACnn'nnGTG
Cuts at: 919 919
Size: 5570
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2155 2187 2155
Size: 32 5538
AlwNI CAG_nnn'CTG
Cuts at: 2875 4442 2875
Size: 1567 4003
ApaI G_GGCC'C
Cuts at: 1242 1242
Size: 5570
ApaLI G'TGCA_C
Cuts at: 4340 4340
Size: 5570
ApoI r'AATT_y
Cuts at: 536 671 936 2155 2313 2574 4923 5179 536
Size: 135 265 1219 158 261 2349 256 927
Fragments arranged by size:
2349 1219 927 265 261 256 158 135
AseI AT'TA_AT
Cuts at: 20 20
Size: 5570
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5570
AvaI C'yCGr_G
Cuts at: 298 305 2590 298
Size: 7 2285 3278
Fragments arranged by size:
3278 2285 7
BamHI G'GATC_C
Cuts at: 2595 2595
Size: 5570
BanI G'GyrC_C
Cuts at: 963 1387 2265 2586 3000 3248 3283 963
Size: 424 878 321 414 248 35 3250
Fragments arranged by size:
3250 878 424 414 321 248 35
BanII G_rGCy'C
Cuts at: 134 1131 1242 2050 2136 2584 3614 134
Size: 997 111 808 86 448 1030 2090
Fragments arranged by size:
2090 1030 997 808 448 111 86
BbeI G_GCGC'C
Cuts at: 3004 3252 3004
Size: 248 5322
BbsI GAAGACnn'nnnn_
Cuts at: 572 1451 572
Size: 879 4691
BbvCI CC'TCA_GC
Cuts at: 482 1645 482
Size: 1163 4407
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2523 2557 2523
Size: 34 5536
BciVI GTATCCnnnnn_n'
Cuts at: 105 3468 4229 5570 105
Size: 3363 761 1341 105
Fragments arranged by size:
3363 1341 761 105
BclI T'GATC_A
Cuts at: 2192 3090 2192
Size: 898 4672
BglII A'GATC_T
Cuts at: 3085 5122 3085
Size: 2037 3533
BlpI GC'TnA_GC
Cuts at: 1637 2044 2682 1637
Size: 407 638 4525
Fragments arranged by size:
4525 638 407
Bme1580I G_kGCm'C
Cuts at: 1242 2073 2270 3195 3288 4344 1242
Size: 831 197 925 93 1056 2468
Fragments arranged by size:
2468 1056 925 831 197 93
BmgBI CAC'GTC
Cuts at: 260 804 260
Size: 544 5026
BmrI ACTGGGnnnn_n'
Cuts at: 2782 2863 3198 5342 2782
Size: 81 335 2144 3010
Fragments arranged by size:
3010 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 192 3937 192
Size: 3745 1825
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1735 1913 2044 1735
Size: 178 131 5261
Fragments arranged by size:
5261 178 131
Bpu10I CC'TnA_GC
Cuts at: 482 1645 482
Size: 1163 4407
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 569 913 2120 2380 2746 3625 4117 4415
Size: 172 344 1207 260 366 879 492 298
Cuts at: 4415 397
Size: 1552
Fragments arranged by size:
1552 1207 879 492 366 344 298 260
172
BsaI GGTCTCn'nnnn_
Cuts at: 308 1088 5274 308
Size: 780 4186 604
Fragments arranged by size:
4186 780 604
BsaAI yAC'GTr
Cuts at: 2923 2923
Size: 5570
BsaBI GATnn'nnATC
Cuts at: 6 3084 5121 6
Size: 3078 2037 455
Fragments arranged by size:
3078 2037 455
BsaHI Gr'CG_yC
Cuts at: 286 569 1301 3001 3249 3951 5447 286
Size: 283 732 1700 248 702 1496 409
Fragments arranged by size:
1700 1496 732 702 409 283 248
BsaWI w'CCGG_w
Cuts at: 2759 3280 3958 4232 4379 4790 2759
Size: 521 678 274 147 411 3539
Fragments arranged by size:
3539 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 490 520 490
Size: 30 5540
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 286 499 1633 1690 286
Size: 213 1134 57 4166
Fragments arranged by size:
4166 1134 213 57
BseYI C'CCAG_C
Cuts at: 332 950 954 1130 1140 1200 2429 2995 3753
Size: 618 4 176 10 60 1229 566 758
Cuts at: 3753 4330 4826 332
Size: 577 496 1076
Fragments arranged by size:
1229 1076 758 618 577 566 496 176
60 10 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 374 526 2563 374
Size: 152 2037 3381
Fragments arranged by size:
3381 2037 152
BsiEI CG_ry'CG
Cuts at: 104 3112 3158 4366 104
Size: 3008 46 1208 1308
Fragments arranged by size:
3008 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 134 1638 2584 3362 3552 4344 134
Size: 1504 946 778 190 792 1360
Fragments arranged by size:
1504 1360 946 792 778 190
BsmI GAATG_Cn'
Cuts at: 2013 2013
Size: 5570
BsmBI CGTCTCn'nnnn_
Cuts at: 262 262
Size: 5570
Bsp1286I G_dGCh'C
Cuts at: 134 1131 1242 1638 2050 2073 2136 2270 2584
Size: 997 111 396 412 23 63 134 314
Cuts at: 2584 3195 3288 3362 3552 3614 4344 134
Size: 611 93 74 190 62 730 1360
Fragments arranged by size:
1360 997 730 611 412 396 314 190
134 111 93 74 63 62 23
BspEI T'CCGG_A
Cuts at: 4790 4790
Size: 5570
BspHI T'CATG_A
Cuts at: 2558 4746 2558
Size: 2188 3382
BspMI ACCTGCnnnn'nnnn_
Cuts at: 716 1106 2620 2834 3136 3517 716
Size: 390 1514 214 302 381 2769
Fragments arranged by size:
2769 1514 390 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1557 3862 3916 5400 5565 50
Size: 1507 2305 54 1484 165 55
Fragments arranged by size:
2305 1507 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2114 3482 2114
Size: 1368 4202
BsrFI r'CCGG_y
Cuts at: 816 3568 3749 3958 816
Size: 2752 181 209 2428
Fragments arranged by size:
2752 2428 209 181
BsrGI T'GTAC_A
Cuts at: 1709 1709
Size: 5570
BssSI C'ACGA_G
Cuts at: 1651 3841 4199 1651
Size: 2190 358 3022
Fragments arranged by size:
3022 2190 358
BstAPI GCAn_nnn'nTGC
Cuts at: 549 826 1534 2947 549
Size: 277 708 1413 3172
Fragments arranged by size:
3172 1413 708 277
BstBI TT'CG_AA
Cuts at: 987 3931 987
Size: 2944 2626
BstEII G'GTnAC_C
Cuts at: 1660 1660
Size: 5570
BstXI CCAn_nnnn'nTGG
Cuts at: 1657 1657
Size: 5570
BstYI r'GATC_y
Cuts at: 1 212 1257 2595 3062 3085 3420 3666 4678
Size: 211 1045 1338 467 23 335 246 1012
Cuts at: 4678 4764 4776 5122 1
Size: 86 12 346 449
Fragments arranged by size:
1338 1045 1012 467 449 346 335 246
211 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1243 1768 1243
Size: 525 5045
BtgI C'CryG_G
Cuts at: 107 782 1730 107
Size: 675 948 3947
Fragments arranged by size:
3947 948 675
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3520 3701 3864 3520
Size: 181 163 5226
Fragments arranged by size:
5226 181 163
BtsI GCAGTG_nn'
Cuts at: 547 2813 547
Size: 2266 3304
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 348 383 348
Size: 35 5535
DraI TTT'AAA
Cuts at: 1152 1616 2569 1152
Size: 464 953 4153
Fragments arranged by size:
4153 953 464
DrdI GACnn_nn'nnGTC
Cuts at: 626 3276 4134 626
Size: 2650 858 2062
Fragments arranged by size:
2650 2062 858
EaeI y'GGCC_r
Cuts at: 3155 3329 3720 3747 5092 3155
Size: 174 391 27 1345 3633
Fragments arranged by size:
3633 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3155 3155
Size: 5570
EarI CTCTTCn'nnn_
Cuts at: 446 457 1970 2285 3593 3803 446
Size: 11 1513 315 1308 210 2213
Fragments arranged by size:
2213 1513 1308 315 210 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1342 3778 4088 4234 5380 1342
Size: 2436 310 146 1146 1532
Fragments arranged by size:
2436 1532 1146 310 146
EcoICRI GAG'CTC
Cuts at: 132 2582 132
Size: 2450 3120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 121 433 853 1375 1624 1735 1913 2044 2116
Size: 312 420 522 249 111 178 131 72
Cuts at: 2116 3395 3827 4574 4842 121
Size: 1279 432 747 268 849
Fragments arranged by size:
1279 849 747 522 432 420 312 268
249 178 131 111 72
EcoNI CCTnn'n_nnAGG
Cuts at: 2275 2275
Size: 5570
EcoO109I rG'GnC_Cy
Cuts at: 467 619 1238 1239 1746 2426 2553 2709 467
Size: 152 619 1 507 680 127 156 3328
Fragments arranged by size:
3328 680 619 507 156 152 127 1
EcoRI G'AATT_C
Cuts at: 536 2574 536
Size: 2038 3532
EcoRV GAT'ATC
Cuts at: 2244 2244
Size: 5570
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 486 589 2371 3589 486
Size: 103 1782 1218 2467
Fragments arranged by size:
2467 1782 1218 103
FspI TGC'GCA
Cuts at: 859 3351 859
Size: 2492 3078
FspAI rTGC'GCAy
Cuts at: 859 859
Size: 5570
HaeII r_GCGC'y
Cuts at: 234 1069 2256 3004 3252 4274 5253 234
Size: 835 1187 748 248 1022 979 551
Fragments arranged by size:
1187 1022 979 835 748 551 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1286 1318 2437 2469 3412 3442 3444 3474 1286
Size: 32 1119 32 943 30 2 30 3382
Fragments arranged by size:
3382 1119 943 32 32 30 30 2
HincII GTy'rAC
Cuts at: 812 2609 812
Size: 1797 3773
KasI G'GCGC_C
Cuts at: 3000 3248 3000
Size: 248 5322
KpnI G_GTAC'C
Cuts at: 2590 2590
Size: 5570
MfeI C'AATT_G
Cuts at: 979 979
Size: 5570
MluI A'CGCG_T
Cuts at: 5003 5003
Size: 5570
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 794 1421 2105 4241 4425 794
Size: 627 684 2136 184 1939
Fragments arranged by size:
2136 1939 684 627 184
MscI TGG'CCA
Cuts at: 3331 3331
Size: 5570
MslI CAynn'nnrTG
Cuts at: 652 919 3559 3686 652
Size: 267 2640 127 2536
Fragments arranged by size:
2640 2536 267 127
MspA1I CmG'CkG
Cuts at: 954 1140 2681 2995 3355 4368 4613 954
Size: 186 1541 314 360 1013 245 1911
Fragments arranged by size:
1911 1541 1013 360 314 245 186
NaeI GCC'GGC
Cuts at: 3751 3751
Size: 5570
NarI GG'CG_CC
Cuts at: 3001 3249 3001
Size: 248 5322
NcoI C'CATG_G
Cuts at: 107 782 107
Size: 675 4895
NgoMIV G'CCGG_C
Cuts at: 3749 3749
Size: 5570
NspI r_CATG'y
Cuts at: 777 1086 1803 2623 3123 4030 777
Size: 309 717 820 500 907 2317
Fragments arranged by size:
2317 907 820 717 500 309
PciI A'CATG_T
Cuts at: 773 1799 4026 773
Size: 1026 2227 2317
Fragments arranged by size:
2317 2227 1026
PflMI CCAn_nnn'nTGG
Cuts at: 339 339
Size: 5570
PfoI T'CCnGG_A
Cuts at: 457 1163 457
Size: 706 4864
PmeI GTTT'AAAC
Cuts at: 2569 2569
Size: 5570
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4709 4741 4709
Size: 32 5538
PpuMI rG'GwC_Cy
Cuts at: 467 619 2553 467
Size: 152 1934 3484
Fragments arranged by size:
3484 1934 152
PsiI TTA'TAA
Cuts at: 905 905
Size: 5570
PspOMI G'GGCC_C
Cuts at: 1238 1238
Size: 5570
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 188 220 188
Size: 32 5538
PstI C_TGCA'G
Cuts at: 713 2617 3302 713
Size: 1904 685 2981
Fragments arranged by size:
2981 1904 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5570
PvuII CAG'CTG
Cuts at: 954 1140 2995 3355 954
Size: 186 1855 360 3169
Fragments arranged by size:
3169 1855 360 186
RsrII CG'GwC_CG
Cuts at: 3765 3765
Size: 5570
SacI G_AGCT'C
Cuts at: 134 2584 134
Size: 2450 3120
SalI G'TCGA_C
Cuts at: 2607 2607
Size: 5570
SanDI GG'GwC_CC
Cuts at: 619 619
Size: 5570
SapI GCTCTTCn'nnn_
Cuts at: 3593 3803 3593
Size: 210 5360
SbfI CC_TGCA'GG
Cuts at: 2617 2617
Size: 5570
ScaI AGT'ACT
Cuts at: 1756 5087 1756
Size: 3331 2239
SexAI A'CCwGG_T
Cuts at: 719 1156 719
Size: 437 5133
SfcI C'TryA_G
Cuts at: 33 709 1030 2613 3298 4291 4482 33
Size: 676 321 1583 685 993 191 1121
Fragments arranged by size:
1583 1121 993 685 676 321 191
SfoI GGC'GCC
Cuts at: 3002 3250 3002
Size: 248 5322
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5570
SmaI CCC'GGG
Cuts at: 2592 2592
Size: 5570
SmlI C'TyrA_G
Cuts at: 376 584 892 2135 2395 2725 3640 4132 4394
Size: 208 308 1243 260 330 915 492 262
Cuts at: 4394 376
Size: 1552
Fragments arranged by size:
1552 1243 915 492 330 308 262 260
208
SpeI A'CTAG_T
Cuts at: 67 1912 67
Size: 1845 3725
SphI G_CATG'C
Cuts at: 1086 2623 3123 1086
Size: 1537 500 3533
Fragments arranged by size:
3533 1537 500
StuI AGG'CCT
Cuts at: 880 880
Size: 5570
StyI C'CwwG_G
Cuts at: 107 782 881 1738 2704 3058 107
Size: 675 99 857 966 354 2619
Fragments arranged by size:
2619 966 857 675 354 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3153 3286 3745 3954 3153
Size: 133 459 209 4769
Fragments arranged by size:
4769 459 209 133
TatI w'GTAC_w
Cuts at: 1169 1277 1709 1754 5085 1169
Size: 108 432 45 3331 1654
Fragments arranged by size:
3331 1654 432 108 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1333 1365 5087 5119 1333
Size: 32 3722 32 1784
Fragments arranged by size:
3722 1784 32 32
Tth111I GACn'n_nGTC
Cuts at: 3367 3367
Size: 5570
XbaI T'CTAG_A
Cuts at: 2601 2601
Size: 5570
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2535 3065 2535
Size: 530 5040
XmaI C'CCGG_G
Cuts at: 2590 2590
Size: 5570
XmnI GAAnn'nnTTC
Cuts at: 2351 2351
Size: 5570
ZraI GAC'GTC
Cuts at: 1302 1302
Size: 5570
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI FspAI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PpuMI PsiI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AflII AhdI AlfI AscI AvrII BaeI BfrBI BglI
BmtI BsiWI BssHII BstZ17I ClaI DraIII FseI HindIII
HpaI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PshAI PspXI SacII SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI