Restriction map for
pF1KA0099
(Circular) (Six-base) MAPSORT of: pF1KA0099.seq Check: 3975 from: 1 to: 6599
pF1KA0099 6599 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AatII G_ACGT'C
Cuts at: 983 983
Size: 6599
AccI GT'mk_AC
Cuts at: 1514 3637 1514
Size: 2123 4476
Acc65I G'GTAC_C
Cuts at: 1206 2549 3615 1206
Size: 1343 1066 4190
Fragments arranged by size:
4190 1343 1066
AclI AA'CG_TT
Cuts at: 6439 6439
Size: 6599
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1165 2423 2427 2852 4424 4856 5603 5871 1165
Size: 1258 4 425 1572 432 747 268 1893
Fragments arranged by size:
1893 1572 1258 747 432 425 268 4
AfeI AGC'GCT
Cuts at: 1407 1407
Size: 6599
AflIII A'CryG_T
Cuts at: 1161 3177 3951 5055 6032 1161
Size: 2016 774 1104 977 1728
Fragments arranged by size:
2016 1728 1104 977 774
AgeI A'CCGG_T
Cuts at: 2546 4987 2546
Size: 2441 4158
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1343 1559 3281 1343
Size: 216 1722 4661
Fragments arranged by size:
4661 1722 216
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1829 1861 1829
Size: 32 6567
AlwNI CAG_nnn'CTG
Cuts at: 635 1231 2401 2772 3904 5471 635
Size: 596 1170 371 1132 1567 1763
Fragments arranged by size:
1763 1567 1170 1132 596 371
ApaI G_GGCC'C
Cuts at: 3566 3566
Size: 6599
ApaLI G'TGCA_C
Cuts at: 3339 5369 3339
Size: 2030 4569
ApoI r'AATT_y
Cuts at: 1710 2593 2749 3262 3603 5952 6208 1710
Size: 883 156 513 341 2349 256 2101
Fragments arranged by size:
2349 2101 883 513 341 256 156
AseI AT'TA_AT
Cuts at: 20 2885 20
Size: 2865 3734
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6599
AvaI C'yCGr_G
Cuts at: 795 2448 3619 795
Size: 1653 1171 3775
Fragments arranged by size:
3775 1653 1171
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1190 1223 1190
Size: 33 6566
BamHI G'GATC_C
Cuts at: 1683 2086 2329 3624 1683
Size: 403 243 1295 4658
Fragments arranged by size:
4658 1295 403 243
BanI G'GyrC_C
Cuts at: 1096 1206 1798 2074 2549 3615 4029 4277 4312
Size: 110 592 276 475 1066 414 248 35
Cuts at: 4312 1096
Size: 3383
Fragments arranged by size:
3383 1066 592 475 414 276 248 110
35
BanII G_rGCy'C
Cuts at: 790 1842 2023 3566 3613 4643 790
Size: 1052 181 1543 47 1030 2746
Fragments arranged by size:
2746 1543 1052 1030 181 47
BbeI G_GCGC'C
Cuts at: 2078 4033 4281 2078
Size: 1955 248 4396
Fragments arranged by size:
4396 1955 248
BbsI GAAGACnn'nnnn_
Cuts at: 690 2664 690
Size: 1974 4625
BbvCI CC'TCA_GC
Cuts at: 1884 1913 1884
Size: 29 6570
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2052 2086 2052
Size: 34 6565
BciVI GTATCCnnnnn_n'
Cuts at: 4497 5258 6599 4497
Size: 761 1341 4497
Fragments arranged by size:
4497 1341 761
BclI T'GATC_A
Cuts at: 615 4119 615
Size: 3504 3095
BfrBI ATG'CAT
Cuts at: 2966 3459 2966
Size: 493 6106
BglII A'GATC_T
Cuts at: 4114 6151 4114
Size: 2037 4562
BlpI GC'TnA_GC
Cuts at: 1071 1313 1598 2657 3711 1071
Size: 242 285 1059 1054 3959
Fragments arranged by size:
3959 1059 1054 285 242
Bme1580I G_kGCm'C
Cuts at: 1803 3343 3566 4224 4317 5373 1803
Size: 1540 223 658 93 1056 3029
Fragments arranged by size:
3029 1540 1056 658 223 93
BmgBI CAC'GTC
Cuts at: 2802 3426 2802
Size: 624 5975
BmrI ACTGGGnnnn_n'
Cuts at: 452 1861 2979 3811 3892 4227 6371 452
Size: 1409 1118 832 81 335 2144 680
Fragments arranged by size:
2144 1409 1118 832 680 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 559 1134 2474 4966 559
Size: 575 1340 2492 2192
Fragments arranged by size:
2492 2192 1340 575
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 276 538 2378 2418 2659 2671 3097 3205 3547
Size: 262 1840 40 241 12 426 108 342
Cuts at: 3547 276
Size: 3328
Fragments arranged by size:
3328 1840 426 342 262 241 108 40
12
Bpu10I CC'TnA_GC
Cuts at: 1884 1913 2233 1884
Size: 29 320 6250
Fragments arranged by size:
6250 320 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 170 2875 3775 4654 5146 5444 170
Size: 2705 900 879 492 298 1325
Fragments arranged by size:
2705 1325 900 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 6303 6303
Size: 6599
BsaAI yAC'GTr
Cuts at: 2952 3307 3405 3952 2952
Size: 355 98 547 5599
Fragments arranged by size:
5599 547 355 98
BsaBI GATnn'nnATC
Cuts at: 6 4113 6150 6
Size: 4107 2037 455
Fragments arranged by size:
4107 2037 455
BsaHI Gr'CG_yC
Cuts at: 980 2075 4030 4278 4980 6476 980
Size: 1095 1955 248 702 1496 1103
Fragments arranged by size:
1955 1496 1103 1095 702 248
BsaWI w'CCGG_w
Cuts at: 1761 2546 3788 4309 4987 5261 5408 5819 1761
Size: 785 1242 521 678 274 147 411 2541
Fragments arranged by size:
2541 1242 785 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 766 796 1829 1859 2292 2322 766
Size: 30 1033 30 433 30 5043
Fragments arranged by size:
5043 1033 433 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 407 419 934 1643 2235 3130 407
Size: 12 515 709 592 895 3876
Fragments arranged by size:
3876 895 709 592 515 12
BseYI C'CCAG_C
Cuts at: 280 662 1076 1467 1919 1925 2027 4024 4782
Size: 382 414 391 452 6 102 1997 758
Cuts at: 4782 5359 5855 280
Size: 577 496 1024
Fragments arranged by size:
1997 1024 758 577 496 452 414 391
382 102 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 697 979 1874 697
Size: 282 895 5422
Fragments arranged by size:
5422 895 282
BsiEI CG_ry'CG
Cuts at: 104 742 3195 4141 4187 5395 104
Size: 638 2453 946 46 1208 1308
Fragments arranged by size:
2453 1308 1208 946 638 46
BsiHKAI G_wGCw'C
Cuts at: 790 1842 3083 3191 3326 3343 3613 4391 4581
Size: 1052 1241 108 135 17 270 778 190
Cuts at: 4581 5373 790
Size: 792 2016
Fragments arranged by size:
2016 1241 1052 792 778 270 190 135
108 17
BsiWI C'GTAC_G
Cuts at: 3307 3307
Size: 6599
BsmI GAATG_Cn'
Cuts at: 1818 1818
Size: 6599
Bsp1286I G_dGCh'C
Cuts at: 790 1803 1842 2023 3083 3191 3326 3343 3566
Size: 1013 39 181 1060 108 135 17 223
Cuts at: 3566 3613 4224 4317 4391 4581 4643 5373 790
Size: 47 611 93 74 190 62 730 2016
Fragments arranged by size:
2016 1060 1013 730 611 223 190 181
135 108 93 74 62 47 39 17
BspEI T'CCGG_A
Cuts at: 5819 5819
Size: 6599
BspHI T'CATG_A
Cuts at: 495 5775 495
Size: 5280 1319
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1194 1351 1605 2868 3649 3863 4165 4546 1194
Size: 157 254 1263 781 214 302 381 3247
Fragments arranged by size:
3247 1263 781 381 302 254 214 157
BsrBI CCG'CTC
Cuts at: 50 4891 4945 6429 6594 50
Size: 4841 54 1484 165 55
Fragments arranged by size:
4841 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3243 3279 4511 3243
Size: 36 1232 5331
Fragments arranged by size:
5331 1232 36
BsrFI r'CCGG_y
Cuts at: 2546 4597 4778 4987 2546
Size: 2051 181 209 4158
Fragments arranged by size:
4158 2051 209 181
BsrGI T'GTAC_A
Cuts at: 2976 3144 2976
Size: 168 6431
BssHII G'CGCG_C
Cuts at: 3315 3315
Size: 6599
BssSI C'ACGA_G
Cuts at: 4870 5228 4870
Size: 358 6241
BstAPI GCAn_nnn'nTGC
Cuts at: 546 3976 546
Size: 3430 3169
BstBI TT'CG_AA
Cuts at: 2538 4960 2538
Size: 2422 4177
BstXI CCAn_nnnn'nTGG
Cuts at: 1107 1492 3405 1107
Size: 385 1913 4301
Fragments arranged by size:
4301 1913 385
BstYI r'GATC_y
Cuts at: 1 307 1035 1683 2086 2329 3464 3624 4091
Size: 306 728 648 403 243 1135 160 467
Cuts at: 4091 4114 4449 4695 5707 5793 5805 6151 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1135 1012 728 648 467 449 403 346
335 306 246 243 160 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3200 3200
Size: 6599
BtgI C'CryG_G
Cuts at: 479 1154 1623 2258 479
Size: 675 469 635 4820
Fragments arranged by size:
4820 675 635 469
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 544 1341 3462 4549 4730 4893 544
Size: 797 2121 1087 181 163 2250
Fragments arranged by size:
2250 2121 1087 797 181 163
BtsI GCAGTG_nn'
Cuts at: 146 1712 2142 2175 2376 2424 3842 146
Size: 1566 430 33 201 48 1418 2903
Fragments arranged by size:
2903 1566 1418 430 201 48 33
ClaI AT'CG_AT
Cuts at: 432 3005 3329 432
Size: 2573 324 3702
Fragments arranged by size:
3702 2573 324
DraI TTT'AAA
Cuts at: 3598 3598
Size: 6599
DrdI GACnn_nn'nnGTC
Cuts at: 4305 5163 4305
Size: 858 5741
EaeI y'GGCC_r
Cuts at: 2910 3518 4184 4358 4749 4776 6121 2910
Size: 608 666 174 391 27 1345 3388
Fragments arranged by size:
3388 1345 666 608 391 174 27
EagI C'GGCC_G
Cuts at: 4184 4184
Size: 6599
EarI CTCTTCn'nnn_
Cuts at: 124 519 1184 2014 2325 2340 2446 2472 3491
Size: 395 665 830 311 15 106 26 1019
Cuts at: 3491 4622 4832 124
Size: 1131 210 1891
Fragments arranged by size:
1891 1131 1019 830 665 395 311 210
106 26 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2079 3445 4807 5117 5263 6409 2079
Size: 1366 1362 310 146 1146 2269
Fragments arranged by size:
2269 1366 1362 1146 310 146
EcoICRI GAG'CTC
Cuts at: 788 1840 3611 788
Size: 1052 1771 3776
Fragments arranged by size:
3776 1771 1052
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 276 538 1165 2378 2418 2423 2427 2659 2671
Size: 262 627 1213 40 5 4 232 12
Cuts at: 2671 2852 3097 3205 3547 4424 4856 5603 5871
Size: 181 245 108 342 877 432 747 268
Cuts at: 5871 276
Size: 1004
Fragments arranged by size:
1213 1004 877 747 627 432 342 268
262 245 232 181 108 40 12 5
4
EcoNI CCTnn'n_nnAGG
Cuts at: 302 302
Size: 6599
EcoO109I rG'GnC_Cy
Cuts at: 3562 3738 3562
Size: 176 6423
EcoRI G'AATT_C
Cuts at: 1710 3603 1710
Size: 1893 4706
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 160 1054 4618 160
Size: 894 3564 2141
Fragments arranged by size:
3564 2141 894
FspI TGC'GCA
Cuts at: 4380 4380
Size: 6599
HaeII r_GCGC'y
Cuts at: 1409 2078 2402 2432 2664 4033 4281 5303 6282
Size: 669 324 30 232 1369 248 1022 979
Cuts at: 6282 1409
Size: 1726
Fragments arranged by size:
1726 1369 1022 979 669 324 248 232
30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1296 1328 1815 1847 2292 2324 2786 2818 4441
Size: 32 487 32 445 32 462 32 1623
Cuts at: 4441 4471 4473 4503 1296
Size: 30 2 30 3392
Fragments arranged by size:
3392 1623 487 462 445 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1304 3555 3638 1304
Size: 2251 83 4265
Fragments arranged by size:
4265 2251 83
HindIII A'AGCT_T
Cuts at: 2340 2340
Size: 6599
KasI G'GCGC_C
Cuts at: 2074 4029 4277 2074
Size: 1955 248 4396
Fragments arranged by size:
4396 1955 248
KpnI G_GTAC'C
Cuts at: 1210 2553 3619 1210
Size: 1343 1066 4190
Fragments arranged by size:
4190 1343 1066
MluI A'CGCG_T
Cuts at: 6032 6032
Size: 6599
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1145 2150 3198 5270 5454 1145
Size: 1005 1048 2072 184 2290
Fragments arranged by size:
2290 2072 1048 1005 184
MscI TGG'CCA
Cuts at: 2912 3520 4360 2912
Size: 608 840 5151
Fragments arranged by size:
5151 840 608
MslI CAynn'nnrTG
Cuts at: 3047 3379 4588 4715 3047
Size: 332 1209 127 4931
Fragments arranged by size:
4931 1209 332 127
MspA1I CmG'CkG
Cuts at: 259 280 1080 1266 1309 1339 1375 1438 1471
Size: 21 800 186 43 30 36 63 33
Cuts at: 1471 1549 1555 1699 1888 2631 2656 3444 3710
Size: 78 6 144 189 743 25 788 266
Cuts at: 3710 4024 4384 5397 5642 259
Size: 314 360 1013 245 1216
Fragments arranged by size:
1216 1013 800 788 743 360 314 266
245 189 186 144 78 63 43 36
33 30 25 21 6
NaeI GCC'GGC
Cuts at: 4780 4780
Size: 6599
NarI GG'CG_CC
Cuts at: 2075 4030 4278 2075
Size: 1955 248 4396
Fragments arranged by size:
4396 1955 248
NcoI C'CATG_G
Cuts at: 479 1154 479
Size: 675 5924
NgoMIV G'CCGG_C
Cuts at: 4778 4778
Size: 6599
NsiI A_TGCA'T
Cuts at: 2968 3461 2968
Size: 493 6106
NspI r_CATG'y
Cuts at: 122 210 1165 1752 2268 3181 3652 4152 5059
Size: 88 955 587 516 913 471 500 907
Cuts at: 5059 122
Size: 1662
Fragments arranged by size:
1662 955 913 907 587 516 500 471
88
PasI CC'CwG_GG
Cuts at: 1417 1505 2016 2082 1417
Size: 88 511 66 5934
Fragments arranged by size:
5934 511 88 66
PciI A'CATG_T
Cuts at: 1161 3177 5055 1161
Size: 2016 1878 2705
Fragments arranged by size:
2705 2016 1878
PfoI T'CCnGG_A
Cuts at: 2250 3074 2250
Size: 824 5775
PmeI GTTT'AAAC
Cuts at: 3598 3598
Size: 6599
PmlI CAC'GTG
Cuts at: 2952 2952
Size: 6599
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1829 1861 5738 5770 1829
Size: 32 3877 32 2658
Fragments arranged by size:
3877 2658 32 32
PsiI TTA'TAA
Cuts at: 2180 2225 2180
Size: 45 6554
PspOMI G'GGCC_C
Cuts at: 3562 3562
Size: 6599
PstI C_TGCA'G
Cuts at: 1452 1560 1704 3646 4331 1452
Size: 108 144 1942 685 3720
Fragments arranged by size:
3720 1942 685 144 108
PvuI CG_AT'CG
Cuts at: 104 742 104
Size: 638 5961
PvuII CAG'CTG
Cuts at: 259 280 1080 1266 1309 1339 1438 1471 1699
Size: 21 800 186 43 30 99 33 228
Cuts at: 1699 1888 2631 2656 4024 4384 259
Size: 189 743 25 1368 360 2474
Fragments arranged by size:
2474 1368 800 743 360 228 189 186
99 43 33 30 25 21
RsrII CG'GwC_CG
Cuts at: 4794 4794
Size: 6599
SacI G_AGCT'C
Cuts at: 790 1842 3613 790
Size: 1052 1771 3776
Fragments arranged by size:
3776 1771 1052
SalI G'TCGA_C
Cuts at: 3636 3636
Size: 6599
SapI GCTCTTCn'nnn_
Cuts at: 519 4622 4832 519
Size: 4103 210 2286
Fragments arranged by size:
4103 2286 210
SbfI CC_TGCA'GG
Cuts at: 3646 3646
Size: 6599
ScaI AGT'ACT
Cuts at: 3535 6116 3535
Size: 2581 4018
SexAI A'CCwGG_T
Cuts at: 687 687
Size: 6599
SfcI C'TryA_G
Cuts at: 33 311 1448 1556 1700 2818 3642 4327 5320
Size: 278 1137 108 144 1118 824 685 993
Cuts at: 5320 5511 33
Size: 191 1121
Fragments arranged by size:
1137 1121 1118 993 824 685 278 191
144 108
SfoI GGC'GCC
Cuts at: 2076 4031 4279 2076
Size: 1955 248 4396
Fragments arranged by size:
4396 1955 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6599
SmaI CCC'GGG
Cuts at: 797 3621 797
Size: 2824 3775
SmlI C'TyrA_G
Cuts at: 185 2854 3754 4669 5161 5423 185
Size: 2669 900 915 492 262 1361
Fragments arranged by size:
2669 1361 915 900 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6599
SphI G_CATG'C
Cuts at: 1752 2268 3652 4152 1752
Size: 516 1384 500 4199
Fragments arranged by size:
4199 1384 516 500
SspI AAT'ATT
Cuts at: 605 605
Size: 6599
StyI C'CwwG_G
Cuts at: 479 832 1145 1154 2045 2094 3733 4087 479
Size: 353 313 9 891 49 1639 354 2991
Fragments arranged by size:
2991 1639 891 354 353 313 49 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 601 4182 4315 4774 4983 601
Size: 3581 133 459 209 2217
Fragments arranged by size:
3581 2217 459 209 133
TatI w'GTAC_w
Cuts at: 726 2976 3144 3180 3240 3494 3533 6114 726
Size: 2250 168 36 60 254 39 2581 1211
Fragments arranged by size:
2581 2250 1211 254 168 60 39 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6116 6148 6116
Size: 32 6567
Tth111I GACn'n_nGTC
Cuts at: 689 1733 4396 689
Size: 1044 2663 2892
Fragments arranged by size:
2892 2663 1044
XbaI T'CTAG_A
Cuts at: 3630 3630
Size: 6599
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1161 1944 2043 2608 4094 1161
Size: 783 99 565 1486 3666
Fragments arranged by size:
3666 1486 783 565 99
XmaI C'CCGG_G
Cuts at: 795 3619 795
Size: 2824 3775
XmnI GAAnn'nnTTC
Cuts at: 2534 2789 2534
Size: 255 6344
ZraI GAC'GTC
Cuts at: 981 981
Size: 6599
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PciI PfoI PmeI PmlI
PpiI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI AscI AvrII BglI BmtI
BsmBI BstEII BstZ17I CspCI DraIII EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI PacI PflMI
PpuMI PshAI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI