Restriction map for
pF1KA0102
(Circular) (Six-base) MAPSORT of: pF1KA0102.seq Check: 649 from: 1 to: 3791
pF1KA0102 3791 bp
With 182 enzymes: SgfI *
February 15, 2010 17:49 ..
AccI GT'mk_AC
Cuts at: 829 829
Size: 3791
Acc65I G'GTAC_C
Cuts at: 807 807
Size: 3791
AclI AA'CG_TT
Cuts at: 3631 3631
Size: 3791
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1616 2048 2795 3063 1616
Size: 432 747 268 2344
Fragments arranged by size:
2344 747 432 268
AflIII A'CryG_T
Cuts at: 1143 2247 3224 1143
Size: 1104 977 1710
Fragments arranged by size:
1710 1104 977
AgeI A'CCGG_T
Cuts at: 2179 2179
Size: 3791
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 655 687 655
Size: 32 3759
AlwNI CAG_nnn'CTG
Cuts at: 1096 2663 1096
Size: 1567 2224
ApaLI G'TGCA_C
Cuts at: 2561 2561
Size: 3791
ApoI r'AATT_y
Cuts at: 336 795 3144 3400 336
Size: 459 2349 256 727
Fragments arranged by size:
2349 727 459 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3791
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3791
AvaI C'yCGr_G
Cuts at: 811 811
Size: 3791
BamHI G'GATC_C
Cuts at: 561 816 561
Size: 255 3536
BanI G'GyrC_C
Cuts at: 807 1221 1469 1504 807
Size: 414 248 35 3094
Fragments arranged by size:
3094 414 248 35
BanII G_rGCy'C
Cuts at: 805 1835 805
Size: 1030 2761
BbeI G_GCGC'C
Cuts at: 1225 1473 1225
Size: 248 3543
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 510 544 510
Size: 34 3757
BciVI GTATCCnnnnn_n'
Cuts at: 1689 2450 3791 1689
Size: 761 1341 1689
Fragments arranged by size:
1689 1341 761
BclI T'GATC_A
Cuts at: 1311 1311
Size: 3791
BfrBI ATG'CAT
Cuts at: 727 727
Size: 3791
BglI GCCn_nnn'nGGC
Cuts at: 112 206 397 112
Size: 94 191 3506
Fragments arranged by size:
3506 191 94
BglII A'GATC_T
Cuts at: 1306 3343 1306
Size: 2037 1754
BlpI GC'TnA_GC
Cuts at: 903 903
Size: 3791
Bme1580I G_kGCm'C
Cuts at: 1416 1509 2565 1416
Size: 93 1056 2642
Fragments arranged by size:
2642 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1003 1084 1419 3563 1003
Size: 81 335 2144 1231
Fragments arranged by size:
2144 1231 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2158 2158
Size: 3791
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 573 573
Size: 3791
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 967 1846 2338 2636 967
Size: 879 492 298 2122
Fragments arranged by size:
2122 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3495 3495
Size: 3791
BsaAI yAC'GTr
Cuts at: 1144 1144
Size: 3791
BsaBI GATnn'nnATC
Cuts at: 6 1305 3342 6
Size: 1299 2037 455
Fragments arranged by size:
2037 1299 455
BsaHI Gr'CG_yC
Cuts at: 1222 1470 2172 3668 1222
Size: 248 702 1496 1345
Fragments arranged by size:
1496 1345 702 248
BsaWI w'CCGG_w
Cuts at: 980 1501 2179 2453 2600 3011 980
Size: 521 678 274 147 411 1760
Fragments arranged by size:
1760 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 126 156 126
Size: 30 3761
BseYI C'CCAG_C
Cuts at: 1216 1974 2551 3047 1216
Size: 758 577 496 1960
Fragments arranged by size:
1960 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1333 1379 2587 104
Size: 1229 46 1208 1308
Fragments arranged by size:
1308 1229 1208 46
BsiHKAI G_wGCw'C
Cuts at: 805 1583 1773 2565 805
Size: 778 190 792 2031
Fragments arranged by size:
2031 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 805 1416 1509 1583 1773 1835 2565 805
Size: 611 93 74 190 62 730 2031
Fragments arranged by size:
2031 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3011 3011
Size: 3791
BspHI T'CATG_A
Cuts at: 2967 2967
Size: 3791
BspMI ACCTGCnnnn'nnnn_
Cuts at: 841 1055 1357 1738 841
Size: 214 302 381 2894
Fragments arranged by size:
2894 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2083 2137 3621 3786 50
Size: 2033 54 1484 165 55
Fragments arranged by size:
2033 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 683 1703 683
Size: 1020 2771
BsrFI r'CCGG_y
Cuts at: 1789 1970 2179 1789
Size: 181 209 3401
Fragments arranged by size:
3401 209 181
BsrGI T'GTAC_A
Cuts at: 123 370 123
Size: 247 3544
BssSI C'ACGA_G
Cuts at: 529 2062 2420 529
Size: 1533 358 1900
Fragments arranged by size:
1900 1533 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1168 1168
Size: 3791
BstBI TT'CG_AA
Cuts at: 2152 2152
Size: 3791
BstYI r'GATC_y
Cuts at: 1 546 561 816 1283 1306 1641 1887 2899
Size: 545 15 255 467 23 335 246 1012
Cuts at: 2899 2985 2997 3343 1
Size: 86 12 346 449
Fragments arranged by size:
1012 545 467 449 346 335 255 246
86 23 15 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3791
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1741 1922 2085 1741
Size: 181 163 3447
Fragments arranged by size:
3447 181 163
BtsI GCAGTG_nn'
Cuts at: 1034 1034
Size: 3791
DraI TTT'AAA
Cuts at: 790 790
Size: 3791
DrdI GACnn_nn'nnGTC
Cuts at: 1497 2355 1497
Size: 858 2933
EaeI y'GGCC_r
Cuts at: 198 1376 1550 1941 1968 3313 198
Size: 1178 174 391 27 1345 676
Fragments arranged by size:
1345 1178 676 391 174 27
EagI C'GGCC_G
Cuts at: 1376 1376
Size: 3791
EarI CTCTTCn'nnn_
Cuts at: 511 1814 2024 511
Size: 1303 210 2278
Fragments arranged by size:
2278 1303 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1999 2309 2455 3601 1999
Size: 310 146 1146 2189
Fragments arranged by size:
2189 1146 310 146
EcoICRI GAG'CTC
Cuts at: 803 803
Size: 3791
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 573 1616 2048 2795 3063 573
Size: 1043 432 747 268 1301
Fragments arranged by size:
1301 1043 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 930 930
Size: 3791
EcoRI G'AATT_C
Cuts at: 795 795
Size: 3791
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 540 648 1810 540
Size: 108 1162 2521
Fragments arranged by size:
2521 1162 108
FspI TGC'GCA
Cuts at: 1572 1572
Size: 3791
HaeII r_GCGC'y
Cuts at: 1225 1473 2495 3474 1225
Size: 248 1022 979 1542
Fragments arranged by size:
1542 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1633 1663 1665 1695 1633
Size: 30 2 30 3729
Fragments arranged by size:
3729 30 30 2
HincII GTy'rAC
Cuts at: 830 830
Size: 3791
KasI G'GCGC_C
Cuts at: 1221 1469 1221
Size: 248 3543
KpnI G_GTAC'C
Cuts at: 811 811
Size: 3791
MluI A'CGCG_T
Cuts at: 3224 3224
Size: 3791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 194 203 2462 2646 194
Size: 9 2259 184 1339
Fragments arranged by size:
2259 1339 184 9
MscI TGG'CCA
Cuts at: 1552 1552
Size: 3791
MslI CAynn'nnrTG
Cuts at: 1780 1907 1780
Size: 127 3664
MspA1I CmG'CkG
Cuts at: 121 271 582 660 902 1216 1576 2589 2834
Size: 150 311 78 242 314 360 1013 245
Cuts at: 2834 121
Size: 1078
Fragments arranged by size:
1078 1013 360 314 311 245 242 150
78
NaeI GCC'GGC
Cuts at: 1972 1972
Size: 3791
NarI GG'CG_CC
Cuts at: 1222 1470 1222
Size: 248 3543
NcoI C'CATG_G
Cuts at: 107 107
Size: 3791
NdeI CA'TA_TG
Cuts at: 729 729
Size: 3791
NgoMIV G'CCGG_C
Cuts at: 1970 1970
Size: 3791
NsiI A_TGCA'T
Cuts at: 729 729
Size: 3791
NspI r_CATG'y
Cuts at: 844 1344 2251 844
Size: 500 907 2384
Fragments arranged by size:
2384 907 500
PciI A'CATG_T
Cuts at: 2247 2247
Size: 3791
PmeI GTTT'AAAC
Cuts at: 790 790
Size: 3791
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2930 2962 2930
Size: 32 3759
PshAI GACnn'nnGTC
Cuts at: 715 715
Size: 3791
PstI C_TGCA'G
Cuts at: 838 1523 838
Size: 685 3106
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3791
PvuII CAG'CTG
Cuts at: 121 271 582 1216 1576 121
Size: 150 311 634 360 2336
Fragments arranged by size:
2336 634 360 311 150
RsrII CG'GwC_CG
Cuts at: 1986 1986
Size: 3791
SacI G_AGCT'C
Cuts at: 805 805
Size: 3791
SalI G'TCGA_C
Cuts at: 828 828
Size: 3791
SapI GCTCTTCn'nnn_
Cuts at: 511 1814 2024 511
Size: 1303 210 2278
Fragments arranged by size:
2278 1303 210
SbfI CC_TGCA'GG
Cuts at: 838 838
Size: 3791
ScaI AGT'ACT
Cuts at: 3308 3308
Size: 3791
SfcI C'TryA_G
Cuts at: 33 156 551 834 1519 2512 2703 33
Size: 123 395 283 685 993 191 1121
Fragments arranged by size:
1121 993 685 395 283 191 123
SfoI GGC'GCC
Cuts at: 1223 1471 1223
Size: 248 3543
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3791
SmaI CCC'GGG
Cuts at: 813 813
Size: 3791
SmlI C'TyrA_G
Cuts at: 946 1861 2353 2615 946
Size: 915 492 262 2122
Fragments arranged by size:
2122 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3791
SphI G_CATG'C
Cuts at: 844 1344 844
Size: 500 3291
StyI C'CwwG_G
Cuts at: 107 925 1279 107
Size: 818 354 2619
Fragments arranged by size:
2619 818 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1374 1507 1966 2175 1374
Size: 133 459 209 2990
Fragments arranged by size:
2990 459 209 133
TatI w'GTAC_w
Cuts at: 123 370 3306 123
Size: 247 2936 608
Fragments arranged by size:
2936 608 247
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 126 158 180 212 257 289 3308 3340 126
Size: 32 22 32 45 32 3019 32 577
Fragments arranged by size:
3019 577 45 32 32 32 32 22
Tth111I GACn'n_nGTC
Cuts at: 1588 1588
Size: 3791
XbaI T'CTAG_A
Cuts at: 822 822
Size: 3791
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 444 1286 444
Size: 842 2949
XmaI C'CCGG_G
Cuts at: 811 811
Size: 3791
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PciI PmeI PpiI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbsI BbvCI BmgBI BmtI Bpu10I
BseRI BsgI BsiWI BsmI BsmBI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NheI NotI NruI PacI
PasI PflMI PfoI PmlI PpuMI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI