Restriction map for
pF1KA0104
(Circular) (Six-base) MAPSORT of: pF1KA0104.seq Check: 5590 from: 1 to: 3989
pF1KA0104 3989 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AccI GT'mk_AC
Cuts at: 1027 1027
Size: 3989
Acc65I G'GTAC_C
Cuts at: 1005 1005
Size: 3989
AclI AA'CG_TT
Cuts at: 3829 3829
Size: 3989
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1814 2246 2599 3261 1814
Size: 432 353 662 2542
Fragments arranged by size:
2542 662 432 353
AflIII A'CryG_T
Cuts at: 1341 3141 3422 1341
Size: 1800 281 1908
Fragments arranged by size:
1908 1800 281
AgeI A'CCGG_T
Cuts at: 285 285
Size: 3989
AhdI GACnn_n'nnGTC
Cuts at: 583 583
Size: 3989
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 155 155
Size: 3989
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 313 345 313
Size: 32 3957
AlwNI CAG_nnn'CTG
Cuts at: 507 1294 2732 507
Size: 787 1438 1764
Fragments arranged by size:
1764 1438 787
ApaLI G'TGCA_C
Cuts at: 2827 2827
Size: 3989
ApoI r'AATT_y
Cuts at: 343 379 816 904 993 3342 3598 343
Size: 36 437 88 89 2349 256 734
Fragments arranged by size:
2349 734 437 256 89 88 36
AseI AT'TA_AT
Cuts at: 20 20
Size: 3989
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3989
AvaI C'yCGr_G
Cuts at: 614 1009 614
Size: 395 3594
AvrII C'CTAG_G
Cuts at: 150 150
Size: 3989
BamHI G'GATC_C
Cuts at: 1014 1014
Size: 3989
BanI G'GyrC_C
Cuts at: 1005 1419 1667 1702 1005
Size: 414 248 35 3292
Fragments arranged by size:
3292 414 248 35
BanII G_rGCy'C
Cuts at: 260 1003 2033 260
Size: 743 1030 2216
Fragments arranged by size:
2216 1030 743
BbeI G_GCGC'C
Cuts at: 1423 1671 1423
Size: 248 3741
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 244 278 244
Size: 34 3955
BciVI GTATCCnnnnn_n'
Cuts at: 1887 2943 3989 1887
Size: 1056 1046 1887
Fragments arranged by size:
1887 1056 1046
BclI T'GATC_A
Cuts at: 1509 1509
Size: 3989
BglI GCCn_nnn'nGGC
Cuts at: 112 231 112
Size: 119 3870
BglII A'GATC_T
Cuts at: 1504 3541 1504
Size: 2037 1952
BlpI GC'TnA_GC
Cuts at: 1101 1101
Size: 3989
Bme1580I G_kGCm'C
Cuts at: 134 1614 1707 2831 134
Size: 1480 93 1124 1292
Fragments arranged by size:
1480 1292 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 144 254 1201 1282 1617 3761 144
Size: 110 947 81 335 2144 372
Fragments arranged by size:
2144 947 372 335 110 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2356 2356
Size: 3989
Bpu10I CC'TnA_GC
Cuts at: 785 785
Size: 3989
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 354 1165 2044 2758 3056 354
Size: 811 879 714 298 1287
Fragments arranged by size:
1287 879 811 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3693 3693
Size: 3989
BsaAI yAC'GTr
Cuts at: 1342 1342
Size: 3989
BsaBI GATnn'nnATC
Cuts at: 6 1503 3540 6
Size: 1497 2037 455
Fragments arranged by size:
2037 1497 455
BsaHI Gr'CG_yC
Cuts at: 1420 1668 2370 3866 1420
Size: 248 702 1496 1543
Fragments arranged by size:
1543 1496 702 248
BsaWI w'CCGG_w
Cuts at: 285 318 1178 1699 2377 2788 2935 3209 285
Size: 33 860 521 678 411 147 274 1065
Fragments arranged by size:
1065 860 678 521 411 274 147 33
BseYI C'CCAG_C
Cuts at: 803 1414 2172 2837 3245 803
Size: 611 758 665 408 1547
Fragments arranged by size:
1547 758 665 611 408
BsiEI CG_ry'CG
Cuts at: 104 1531 1577 2807 104
Size: 1427 46 1230 1286
Fragments arranged by size:
1427 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 244 1003 1781 1971 2831 244
Size: 759 778 190 860 1402
Fragments arranged by size:
1402 860 778 759 190
BsmI GAATG_Cn'
Cuts at: 518 518
Size: 3989
Bsp1286I G_dGCh'C
Cuts at: 134 244 260 1003 1614 1707 1781 1971 2033
Size: 110 16 743 611 93 74 190 62
Cuts at: 2033 2831 134
Size: 798 1292
Fragments arranged by size:
1292 798 743 611 190 110 93 74
62 16
BspHI T'CATG_A
Cuts at: 2421 2421
Size: 3989
BspMI ACCTGCnnnn'nnnn_
Cuts at: 317 1039 1253 1555 1936 317
Size: 722 214 302 381 2370
Fragments arranged by size:
2370 722 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2281 2335 3819 3984 50
Size: 2231 54 1484 165 55
Fragments arranged by size:
2231 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 119 149 1901 119
Size: 30 1752 2207
Fragments arranged by size:
2207 1752 30
BsrFI r'CCGG_y
Cuts at: 192 285 1987 2168 192
Size: 93 1702 181 2013
Fragments arranged by size:
2013 1702 181 93
BssSI C'ACGA_G
Cuts at: 2260 2968 2260
Size: 708 3281
BstAPI GCAn_nnn'nTGC
Cuts at: 1366 1366
Size: 3989
BstBI TT'CG_AA
Cuts at: 2350 2350
Size: 3989
BstYI r'GATC_y
Cuts at: 1 369 1014 1481 1504 1839 2085 2391 2403
Size: 368 645 467 23 335 246 306 12
Cuts at: 2403 2489 3541 1
Size: 86 1052 449
Fragments arranged by size:
1052 645 467 449 368 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 559 559
Size: 3989
BtgI C'CryG_G
Cuts at: 107 311 895 107
Size: 204 584 3201
Fragments arranged by size:
3201 584 204
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1939 2120 2283 1939
Size: 181 163 3645
Fragments arranged by size:
3645 181 163
BtsI GCAGTG_nn'
Cuts at: 1232 1232
Size: 3989
DraI TTT'AAA
Cuts at: 988 988
Size: 3989
DrdI GACnn_nn'nnGTC
Cuts at: 1695 3039 1695
Size: 1344 2645
EaeI y'GGCC_r
Cuts at: 1574 1748 2139 2166 3511 1574
Size: 174 391 27 1345 2052
Fragments arranged by size:
2052 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1574 1574
Size: 3989
EarI CTCTTCn'nnn_
Cuts at: 528 2012 2222 528
Size: 1484 210 2295
Fragments arranged by size:
2295 1484 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2197 2939 3085 3799 2197
Size: 742 146 714 2387
Fragments arranged by size:
2387 742 714 146
EcoICRI GAG'CTC
Cuts at: 1001 1001
Size: 3989
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1814 2246 2599 3261 1814
Size: 432 353 662 2542
Fragments arranged by size:
2542 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 223 1128 223
Size: 905 3084
EcoRI G'AATT_C
Cuts at: 343 993 343
Size: 650 3339
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2008 2008
Size: 3989
FspI TGC'GCA
Cuts at: 1770 1770
Size: 3989
HaeII r_GCGC'y
Cuts at: 1423 1671 2901 3672 1423
Size: 248 1230 771 1740
Fragments arranged by size:
1740 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 569 601 1831 1861 1863 1893 569
Size: 32 1230 30 2 30 2665
Fragments arranged by size:
2665 1230 32 30 30 2
HincII GTy'rAC
Cuts at: 1028 1028
Size: 3989
KasI G'GCGC_C
Cuts at: 1419 1667 1419
Size: 248 3741
KpnI G_GTAC'C
Cuts at: 1009 1009
Size: 3989
MluI A'CGCG_T
Cuts at: 3422 3422
Size: 3989
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 294 426 2748 2932 294
Size: 132 2322 184 1351
Fragments arranged by size:
2322 1351 184 132
MscI TGG'CCA
Cuts at: 1750 1750
Size: 3989
MslI CAynn'nnrTG
Cuts at: 1978 2105 1978
Size: 127 3862
MspA1I CmG'CkG
Cuts at: 127 472 1100 1414 1774 2558 2803 127
Size: 345 628 314 360 784 245 1313
Fragments arranged by size:
1313 784 628 360 345 314 245
NaeI GCC'GGC
Cuts at: 194 2170 194
Size: 1976 2013
NarI GG'CG_CC
Cuts at: 1420 1668 1420
Size: 248 3741
NcoI C'CATG_G
Cuts at: 107 895 107
Size: 788 3201
NdeI CA'TA_TG
Cuts at: 480 480
Size: 3989
NgoMIV G'CCGG_C
Cuts at: 192 2168 192
Size: 1976 2013
NspI r_CATG'y
Cuts at: 1042 1542 3145 1042
Size: 500 1603 1886
Fragments arranged by size:
1886 1603 500
PciI A'CATG_T
Cuts at: 3141 3141
Size: 3989
PflMI CCAn_nnn'nTGG
Cuts at: 507 507
Size: 3989
PfoI T'CCnGG_A
Cuts at: 620 620
Size: 3989
PmeI GTTT'AAAC
Cuts at: 988 988
Size: 3989
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 357 389 2435 2467 357
Size: 32 2046 32 1879
Fragments arranged by size:
2046 1879 32 32
PsiI TTA'TAA
Cuts at: 608 608
Size: 3989
PstI C_TGCA'G
Cuts at: 211 1036 1721 211
Size: 825 685 2479
Fragments arranged by size:
2479 825 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3989
PvuII CAG'CTG
Cuts at: 472 1414 1774 472
Size: 942 360 2687
Fragments arranged by size:
2687 942 360
RsrII CG'GwC_CG
Cuts at: 2184 2184
Size: 3989
SacI G_AGCT'C
Cuts at: 1003 1003
Size: 3989
SalI G'TCGA_C
Cuts at: 1026 1026
Size: 3989
SapI GCTCTTCn'nnn_
Cuts at: 2012 2222 2012
Size: 210 3779
SbfI CC_TGCA'GG
Cuts at: 211 1036 211
Size: 825 3164
ScaI AGT'ACT
Cuts at: 422 3506 422
Size: 3084 905
SfcI C'TryA_G
Cuts at: 33 207 467 1032 1717 2685 2876 33
Size: 174 260 565 685 968 191 1146
Fragments arranged by size:
1146 968 685 565 260 191 174
SfoI GGC'GCC
Cuts at: 1421 1669 1421
Size: 248 3741
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3989
SmaI CCC'GGG
Cuts at: 1011 1011
Size: 3989
SmlI C'TyrA_G
Cuts at: 333 1144 2059 2773 3035 333
Size: 811 915 714 262 1287
Fragments arranged by size:
1287 915 811 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3989
SphI G_CATG'C
Cuts at: 1042 1542 1042
Size: 500 3489
SspI AAT'ATT
Cuts at: 825 825
Size: 3989
StyI C'CwwG_G
Cuts at: 107 150 895 1123 1477 107
Size: 43 745 228 354 2619
Fragments arranged by size:
2619 745 354 228 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1572 1705 2164 2373 1572
Size: 133 459 209 3188
Fragments arranged by size:
3188 459 209 133
TatI w'GTAC_w
Cuts at: 420 729 3504 420
Size: 309 2775 905
Fragments arranged by size:
2775 905 309
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3506 3538 3506
Size: 32 3957
Tth111I GACn'n_nGTC
Cuts at: 1786 1786
Size: 3989
XbaI T'CTAG_A
Cuts at: 1020 1020
Size: 3989
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1484 1484
Size: 3989
XmaI C'CCGG_G
Cuts at: 1009 1009
Size: 3989
XmnI GAAnn'nnTTC
Cuts at: 347 347
Size: 3989
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI ApaI AscI BaeI
BbsI BbvCI BfrBI BmgBI BmtI BpmI BsaXI BseRI
BsgI BsiWI BsmBI BspEI BsrGI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NheI NotI NruI NsiI PacI
PasI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI ZraI