Restriction map for
pF1KA0107
(Circular) (Six-base) MAPSORT of: pF1KA0107.seq Check: 5111 from: 1 to: 4280
pF1KA0107 4280 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AccI GT'mk_AC
Cuts at: 1318 1318
Size: 4280
Acc65I G'GTAC_C
Cuts at: 1296 1296
Size: 4280
AclI AA'CG_TT
Cuts at: 4120 4120
Size: 4280
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2105 2537 2890 3552 2105
Size: 432 353 662 2833
Fragments arranged by size:
2833 662 432 353
AflIII A'CryG_T
Cuts at: 1632 3432 3713 1632
Size: 1800 281 2199
Fragments arranged by size:
2199 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 885 1029 1107 1585 3023 885
Size: 144 78 478 1438 2142
Fragments arranged by size:
2142 1438 478 144 78
ApaLI G'TGCA_C
Cuts at: 3118 3118
Size: 4280
ApoI r'AATT_y
Cuts at: 404 1007 1090 1284 3633 3889 404
Size: 603 83 194 2349 256 795
Fragments arranged by size:
2349 795 603 256 194 83
AseI AT'TA_AT
Cuts at: 20 1268 20
Size: 1248 3032
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4280
AvaI C'yCGr_G
Cuts at: 193 1300 193
Size: 1107 3173
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 144 177 144
Size: 33 4247
BamHI G'GATC_C
Cuts at: 1305 1305
Size: 4280
BanI G'GyrC_C
Cuts at: 1296 1710 1958 1993 1296
Size: 414 248 35 3583
Fragments arranged by size:
3583 414 248 35
BanII G_rGCy'C
Cuts at: 468 1294 2324 468
Size: 826 1030 2424
Fragments arranged by size:
2424 1030 826
BbeI G_GCGC'C
Cuts at: 1714 1962 1714
Size: 248 4032
BbsI GAAGACnn'nnnn_
Cuts at: 856 856
Size: 4280
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 270 304 90
Size: 34 146 34 4066
Fragments arranged by size:
4066 146 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2178 3234 4280 2178
Size: 1056 1046 2178
Fragments arranged by size:
2178 1056 1046
BclI T'GATC_A
Cuts at: 1098 1800 1098
Size: 702 3578
BfrBI ATG'CAT
Cuts at: 1015 1015
Size: 4280
BglII A'GATC_T
Cuts at: 810 1227 1795 3832 810
Size: 417 568 2037 1258
Fragments arranged by size:
2037 1258 568 417
BlpI GC'TnA_GC
Cuts at: 184 1392 184
Size: 1208 3072
Bme1580I G_kGCm'C
Cuts at: 1905 1998 3122 1905
Size: 93 1124 3063
Fragments arranged by size:
3063 1124 93
BmgBI CAC'GTC
Cuts at: 306 306
Size: 4280
BmrI ACTGGGnnnn_n'
Cuts at: 636 1492 1573 1908 4052 636
Size: 856 81 335 2144 864
Fragments arranged by size:
2144 864 856 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2647 2647
Size: 4280
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 145 136
Size: 9 4271
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1456 2335 3049 3347 1456
Size: 879 714 298 2389
Fragments arranged by size:
2389 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3984 3984
Size: 4280
BsaAI yAC'GTr
Cuts at: 1633 1633
Size: 4280
BsaBI GATnn'nnATC
Cuts at: 6 569 1794 3831 6
Size: 563 1225 2037 455
Fragments arranged by size:
2037 1225 563 455
BsaHI Gr'CG_yC
Cuts at: 1711 1959 2661 4157 1711
Size: 248 702 1496 1834
Fragments arranged by size:
1834 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1469 1990 2668 3079 3226 3500 1469
Size: 521 678 411 147 274 2249
Fragments arranged by size:
2249 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 142 145 379 635 142
Size: 3 234 256 3787
Fragments arranged by size:
3787 256 234 3
BseYI C'CCAG_C
Cuts at: 1705 2463 3128 3536 1705
Size: 758 665 408 2449
Fragments arranged by size:
2449 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 239 1822 1868 3098 104
Size: 135 1583 46 1230 1286
Fragments arranged by size:
1583 1286 1230 135 46
BsiHKAI G_wGCw'C
Cuts at: 200 468 1294 2072 2262 3122 200
Size: 268 826 778 190 860 1358
Fragments arranged by size:
1358 860 826 778 268 190
BsmI GAATG_Cn'
Cuts at: 907 907
Size: 4280
BsmBI CGTCTCn'nnnn_
Cuts at: 200 200
Size: 4280
Bsp1286I G_dGCh'C
Cuts at: 200 468 1294 1905 1998 2072 2262 2324 3122
Size: 268 826 611 93 74 190 62 798
Cuts at: 3122 200
Size: 1358
Fragments arranged by size:
1358 826 798 611 268 190 93 74
62
BspHI T'CATG_A
Cuts at: 2712 2712
Size: 4280
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1330 1544 1846 2227 1330
Size: 214 302 381 3383
Fragments arranged by size:
3383 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2572 2626 4110 4275 50
Size: 2522 54 1484 165 55
Fragments arranged by size:
2522 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 422 2192 422
Size: 1770 2510
BsrFI r'CCGG_y
Cuts at: 2278 2459 2278
Size: 181 4099
BssSI C'ACGA_G
Cuts at: 2551 3259 2551
Size: 708 3572
BstAPI GCAn_nnn'nTGC
Cuts at: 1657 1657
Size: 4280
BstBI TT'CG_AA
Cuts at: 2641 2641
Size: 4280
BstEII G'GTnAC_C
Cuts at: 511 511
Size: 4280
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4280
BstYI r'GATC_y
Cuts at: 1 810 1227 1305 1772 1795 2130 2376 2682
Size: 809 417 78 467 23 335 246 306
Cuts at: 2682 2694 2780 3832 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 809 467 449 417 335 306 246
86 78 23 12
Bsu36I CC'TnA_GG
Cuts at: 541 541
Size: 4280
BtgI C'CryG_G
Cuts at: 201 201
Size: 4280
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 425 2230 2411 2574 425
Size: 1805 181 163 2131
Fragments arranged by size:
2131 1805 181 163
BtsI GCAGTG_nn'
Cuts at: 1137 1523 1137
Size: 386 3894
ClaI AT'CG_AT
Cuts at: 984 984
Size: 4280
DraI TTT'AAA
Cuts at: 1279 1279
Size: 4280
DrdI GACnn_nn'nnGTC
Cuts at: 1986 3330 1986
Size: 1344 2936
EaeI y'GGCC_r
Cuts at: 236 1865 2039 2430 2457 3802 236
Size: 1629 174 391 27 1345 714
Fragments arranged by size:
1629 1345 714 391 174 27
EagI C'GGCC_G
Cuts at: 236 1865 236
Size: 1629 2651
EarI CTCTTCn'nnn_
Cuts at: 712 2303 2513 712
Size: 1591 210 2479
Fragments arranged by size:
2479 1591 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2488 3230 3376 4090 2488
Size: 742 146 714 2678
Fragments arranged by size:
2678 742 714 146
EcoICRI GAG'CTC
Cuts at: 466 1292 466
Size: 826 3454
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 145 2105 2537 2890 3552 136
Size: 9 1960 432 353 662 864
Fragments arranged by size:
1960 864 662 432 353 9
EcoNI CCTnn'n_nnAGG
Cuts at: 127 127
Size: 4280
EcoO109I rG'GnC_Cy
Cuts at: 1419 1419
Size: 4280
EcoRI G'AATT_C
Cuts at: 1007 1090 1284 1007
Size: 83 194 4003
Fragments arranged by size:
4003 194 83
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2299 2299
Size: 4280
FspI TGC'GCA
Cuts at: 2061 2061
Size: 4280
HaeII r_GCGC'y
Cuts at: 1714 1962 3192 3963 1714
Size: 248 1230 771 2031
Fragments arranged by size:
2031 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 797 829 2122 2152 2154 2184 797
Size: 32 1293 30 2 30 2893
Fragments arranged by size:
2893 1293 32 30 30 2
HincII GTy'rAC
Cuts at: 1319 1319
Size: 4280
HindIII A'AGCT_T
Cuts at: 603 603
Size: 4280
KasI G'GCGC_C
Cuts at: 1710 1958 1710
Size: 248 4032
KpnI G_GTAC'C
Cuts at: 1300 1300
Size: 4280
MluI A'CGCG_T
Cuts at: 3713 3713
Size: 4280
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3039 3223 3039
Size: 184 4096
MscI TGG'CCA
Cuts at: 2041 2041
Size: 4280
MslI CAynn'nnrTG
Cuts at: 2269 2396 2269
Size: 127 4153
MspA1I CmG'CkG
Cuts at: 114 171 203 700 1026 1391 1705 2065 2849
Size: 57 32 497 326 365 314 360 784
Cuts at: 2849 3094 114
Size: 245 1300
Fragments arranged by size:
1300 784 497 365 360 326 314 245
57 32
NaeI GCC'GGC
Cuts at: 2461 2461
Size: 4280
NarI GG'CG_CC
Cuts at: 1711 1959 1711
Size: 248 4032
NdeI CA'TA_TG
Cuts at: 489 489
Size: 4280
NgoMIV G'CCGG_C
Cuts at: 2459 2459
Size: 4280
NruI TCG'CGA
Cuts at: 410 410
Size: 4280
NsiI A_TGCA'T
Cuts at: 1017 1017
Size: 4280
NspI r_CATG'y
Cuts at: 1333 1833 3436 1333
Size: 500 1603 2177
Fragments arranged by size:
2177 1603 500
PasI CC'CwG_GG
Cuts at: 507 507
Size: 4280
PciI A'CATG_T
Cuts at: 3432 3432
Size: 4280
PmeI GTTT'AAAC
Cuts at: 1279 1279
Size: 4280
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2726 2758 2726
Size: 32 4248
PsiI TTA'TAA
Cuts at: 755 755
Size: 4280
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1186 1218 1186
Size: 32 4248
PstI C_TGCA'G
Cuts at: 1327 2012 1327
Size: 685 3595
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4280
PvuII CAG'CTG
Cuts at: 171 700 1026 1705 2065 171
Size: 529 326 679 360 2386
Fragments arranged by size:
2386 679 529 360 326
RsrII CG'GwC_CG
Cuts at: 2475 2475
Size: 4280
SacI G_AGCT'C
Cuts at: 468 1294 468
Size: 826 3454
SacII CC_GC'GG
Cuts at: 204 204
Size: 4280
SalI G'TCGA_C
Cuts at: 1317 1317
Size: 4280
SapI GCTCTTCn'nnn_
Cuts at: 2303 2513 2303
Size: 210 4070
SbfI CC_TGCA'GG
Cuts at: 1327 1327
Size: 4280
ScaI AGT'ACT
Cuts at: 3797 3797
Size: 4280
SfcI C'TryA_G
Cuts at: 33 1323 2008 2976 3167 33
Size: 1290 685 968 191 1146
Fragments arranged by size:
1290 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1712 1960 1712
Size: 248 4032
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4280
SmaI CCC'GGG
Cuts at: 1302 1302
Size: 4280
SmlI C'TyrA_G
Cuts at: 1435 2350 3064 3326 1435
Size: 915 714 262 2389
Fragments arranged by size:
2389 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4280
SphI G_CATG'C
Cuts at: 1333 1833 1333
Size: 500 3780
StyI C'CwwG_G
Cuts at: 1053 1414 1768 1053
Size: 361 354 3565
Fragments arranged by size:
3565 361 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1863 1996 2455 2664 1863
Size: 133 459 209 3479
Fragments arranged by size:
3479 459 209 133
TatI w'GTAC_w
Cuts at: 3795 3795
Size: 4280
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3797 3829 3797
Size: 32 4248
Tth111I GACn'n_nGTC
Cuts at: 2077 2077
Size: 4280
XbaI T'CTAG_A
Cuts at: 1311 1311
Size: 4280
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1775 1775
Size: 4280
XmaI C'CCGG_G
Cuts at: 1300 1300
Size: 4280
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NruI NsiI NspI
PasI PciI PmeI PpiI PsiI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BbvCI BglI BmtI Bpu10I
BsaXI BsgI BsiWI BspEI BsrGI BssHII BstZ17I CspCI
DraIII EcoRV FseI FspAI HpaI MfeI NcoI NheI
NotI PacI PflMI PfoI PmlI PpuMI PshAI PspOMI
PspXI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI