Restriction map for
pF1KA0128
(Circular) (Six-base) MAPSORT of: pF1KA0128.seq Check: 92 from: 1 to: 4394
pF1KA0128 4394 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 812 812
Size: 4394
AccI GT'mk_AC
Cuts at: 1432 1432
Size: 4394
Acc65I G'GTAC_C
Cuts at: 1410 1410
Size: 4394
AclI AA'CG_TT
Cuts at: 4234 4234
Size: 4394
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 613 859 1044 1240 1384 2219 2651 3004 3666
Size: 246 185 196 144 835 432 353 662
Cuts at: 3666 613
Size: 1341
Fragments arranged by size:
1341 835 662 432 353 246 196 185
144
AflIII A'CryG_T
Cuts at: 171 1746 3546 3827 171
Size: 1575 1800 281 738
Fragments arranged by size:
1800 1575 738 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 277 309 277
Size: 32 4362
AlwNI CAG_nnn'CTG
Cuts at: 1362 1699 3137 1362
Size: 337 1438 2619
Fragments arranged by size:
2619 1438 337
ApaLI G'TGCA_C
Cuts at: 3232 3232
Size: 4394
ApoI r'AATT_y
Cuts at: 304 466 1398 3747 4003 304
Size: 162 932 2349 256 695
Fragments arranged by size:
2349 932 695 256 162
AseI AT'TA_AT
Cuts at: 20 20
Size: 4394
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
AvaI C'yCGr_G
Cuts at: 1414 1414
Size: 4394
AvrII C'CTAG_G
Cuts at: 1101 1101
Size: 4394
BamHI G'GATC_C
Cuts at: 1419 1419
Size: 4394
BanI G'GyrC_C
Cuts at: 1410 1824 2072 2107 1410
Size: 414 248 35 3697
Fragments arranged by size:
3697 414 248 35
BanII G_rGCy'C
Cuts at: 1178 1339 1408 2438 1178
Size: 161 69 1030 3134
Fragments arranged by size:
3134 1030 161 69
BbeI G_GCGC'C
Cuts at: 1828 2076 1828
Size: 248 4146
BciVI GTATCCnnnnn_n'
Cuts at: 884 2292 3348 4394 884
Size: 1408 1056 1046 884
Fragments arranged by size:
1408 1056 1046 884
BclI T'GATC_A
Cuts at: 1914 1914
Size: 4394
BglII A'GATC_T
Cuts at: 740 1909 3946 740
Size: 1169 2037 1188
Fragments arranged by size:
2037 1188 1169
BlpI GC'TnA_GC
Cuts at: 1316 1506 1316
Size: 190 4204
Bme1580I G_kGCm'C
Cuts at: 886 2019 2112 3236 886
Size: 1133 93 1124 2044
Fragments arranged by size:
2044 1133 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1606 1687 2022 4166 1606
Size: 81 335 2144 1834
Fragments arranged by size:
2144 1834 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2761 2761
Size: 4394
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 1036 1096 1273 1309 1367 334
Size: 702 60 177 36 58 3361
Fragments arranged by size:
3361 702 177 60 58 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1570 2449 3163 3461 1570
Size: 879 714 298 2503
Fragments arranged by size:
2503 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4098 4098
Size: 4394
BsaAI yAC'GTr
Cuts at: 1747 1747
Size: 4394
BsaBI GATnn'nnATC
Cuts at: 6 1908 3945 6
Size: 1902 2037 455
Fragments arranged by size:
2037 1902 455
BsaHI Gr'CG_yC
Cuts at: 1825 2073 2775 4271 1825
Size: 248 702 1496 1948
Fragments arranged by size:
1948 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1583 2104 2782 3193 3340 3614 1583
Size: 521 678 411 147 274 2363
Fragments arranged by size:
2363 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 526 556 526
Size: 30 4364
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1033 1141 1033
Size: 108 4286
BseYI C'CCAG_C
Cuts at: 1819 2577 3242 3650 1819
Size: 758 665 408 2563
Fragments arranged by size:
2563 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 910 1183 1213 910
Size: 273 30 4091
Fragments arranged by size:
4091 273 30
BsiEI CG_ry'CG
Cuts at: 104 119 404 1936 1982 3212 104
Size: 15 285 1532 46 1230 1286
Fragments arranged by size:
1532 1286 1230 285 46 15
BsiHKAI G_wGCw'C
Cuts at: 1178 1408 2186 2376 3236 1178
Size: 230 778 190 860 2336
Fragments arranged by size:
2336 860 778 230 190
BsmI GAATG_Cn'
Cuts at: 1293 1293
Size: 4394
Bsp1286I G_dGCh'C
Cuts at: 886 1178 1339 1408 2019 2112 2186 2376 2438
Size: 292 161 69 611 93 74 190 62
Cuts at: 2438 3236 886
Size: 798 2044
Fragments arranged by size:
2044 798 611 292 190 161 93 74
69 62
BspHI T'CATG_A
Cuts at: 2826 2826
Size: 4394
BspMI ACCTGCnnnn'nnnn_
Cuts at: 503 812 882 1444 1658 1960 2341 503
Size: 309 70 562 214 302 381 2556
Fragments arranged by size:
2556 562 381 309 302 214 70
BsrBI CCG'CTC
Cuts at: 50 2686 2740 4224 4389 50
Size: 2636 54 1484 165 55
Fragments arranged by size:
2636 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 569 653 2306 569
Size: 84 1653 2657
Fragments arranged by size:
2657 1653 84
BsrFI r'CCGG_y
Cuts at: 2392 2573 2392
Size: 181 4213
BssSI C'ACGA_G
Cuts at: 1201 2665 3373 1201
Size: 1464 708 2222
Fragments arranged by size:
2222 1464 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1771 1771
Size: 4394
BstBI TT'CG_AA
Cuts at: 308 676 2755 308
Size: 368 2079 1947
Fragments arranged by size:
2079 1947 368
BstXI CCAn_nnnn'nTGG
Cuts at: 282 282
Size: 4394
BstYI r'GATC_y
Cuts at: 1 512 740 960 1419 1886 1909 2244 2490
Size: 511 228 220 459 467 23 335 246
Cuts at: 2490 2796 2808 2894 3946 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 511 467 459 449 335 306 246
228 220 86 23 12
BtgI C'CryG_G
Cuts at: 107 578 107
Size: 471 3923
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2344 2525 2688 2344
Size: 181 163 4050
Fragments arranged by size:
4050 181 163
BtsI GCAGTG_nn'
Cuts at: 909 1637 909
Size: 728 3666
ClaI AT'CG_AT
Cuts at: 473 473
Size: 4394
DraI TTT'AAA
Cuts at: 1393 1393
Size: 4394
DrdI GACnn_nn'nnGTC
Cuts at: 2100 3444 2100
Size: 1344 3050
EaeI y'GGCC_r
Cuts at: 1979 2153 2544 2571 3916 1979
Size: 174 391 27 1345 2457
Fragments arranged by size:
2457 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1979 1979
Size: 4394
EarI CTCTTCn'nnn_
Cuts at: 497 513 673 1118 1360 2417 2627 497
Size: 16 160 445 242 1057 210 2264
Fragments arranged by size:
2264 1057 445 242 210 160 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2602 3344 3490 4204 2602
Size: 742 146 714 2792
Fragments arranged by size:
2792 742 714 146
EcoICRI GAG'CTC
Cuts at: 1176 1406 1176
Size: 230 4164
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 613 859 1036 1044 1096 1240 1273 1309
Size: 279 246 177 8 52 144 33 36
Cuts at: 1309 1367 1384 2219 2651 3004 3666 334
Size: 58 17 835 432 353 662 1062
Fragments arranged by size:
1062 835 662 432 353 279 246 177
144 58 52 36 33 17 8
EcoO109I rG'GnC_Cy
Cuts at: 1533 1533
Size: 4394
EcoRI G'AATT_C
Cuts at: 466 1398 466
Size: 932 3462
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2413 2413
Size: 4394
FspI TGC'GCA
Cuts at: 2175 2175
Size: 4394
HaeII r_GCGC'y
Cuts at: 1828 2076 3306 4077 1828
Size: 248 1230 771 2145
Fragments arranged by size:
2145 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 725 751 757 783 2236 2266 2268 2298 725
Size: 26 6 26 1453 30 2 30 2821
Fragments arranged by size:
2821 1453 30 30 26 26 6 2
HincII GTy'rAC
Cuts at: 951 1433 951
Size: 482 3912
KasI G'GCGC_C
Cuts at: 1824 2072 1824
Size: 248 4146
KpnI G_GTAC'C
Cuts at: 1414 1414
Size: 4394
MluI A'CGCG_T
Cuts at: 3827 3827
Size: 4394
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3153 3337 3153
Size: 184 4210
MscI TGG'CCA
Cuts at: 2155 2155
Size: 4394
MslI CAynn'nnrTG
Cuts at: 280 2383 2510 280
Size: 2103 127 2164
Fragments arranged by size:
2164 2103 127
MspA1I CmG'CkG
Cuts at: 201 1007 1505 1819 2179 2963 3208 201
Size: 806 498 314 360 784 245 1387
Fragments arranged by size:
1387 806 784 498 360 314 245
NaeI GCC'GGC
Cuts at: 2575 2575
Size: 4394
NarI GG'CG_CC
Cuts at: 1825 2073 1825
Size: 248 4146
NcoI C'CATG_G
Cuts at: 107 107
Size: 4394
NdeI CA'TA_TG
Cuts at: 1077 1077
Size: 4394
NgoMIV G'CCGG_C
Cuts at: 2573 2573
Size: 4394
NspI r_CATG'y
Cuts at: 175 1447 1947 3550 175
Size: 1272 500 1603 1019
Fragments arranged by size:
1603 1272 1019 500
PasI CC'CwG_GG
Cuts at: 1332 1332
Size: 4394
PciI A'CATG_T
Cuts at: 171 3546 171
Size: 3375 1019
PmeI GTTT'AAAC
Cuts at: 1393 1393
Size: 4394
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2840 2872 2840
Size: 32 4362
PstI C_TGCA'G
Cuts at: 500 1441 2126 500
Size: 941 685 2768
Fragments arranged by size:
2768 941 685
PvuI CG_AT'CG
Cuts at: 104 404 104
Size: 300 4094
PvuII CAG'CTG
Cuts at: 201 1819 2179 201
Size: 1618 360 2416
Fragments arranged by size:
2416 1618 360
RsrII CG'GwC_CG
Cuts at: 2589 2589
Size: 4394
SacI G_AGCT'C
Cuts at: 1178 1408 1178
Size: 230 4164
SalI G'TCGA_C
Cuts at: 1431 1431
Size: 4394
SapI GCTCTTCn'nnn_
Cuts at: 497 2417 2627 497
Size: 1920 210 2264
Fragments arranged by size:
2264 1920 210
SbfI CC_TGCA'GG
Cuts at: 500 1441 500
Size: 941 3453
ScaI AGT'ACT
Cuts at: 3911 3911
Size: 4394
SfcI C'TryA_G
Cuts at: 33 496 755 1437 2122 3090 3281 33
Size: 463 259 682 685 968 191 1146
Fragments arranged by size:
1146 968 685 682 463 259 191
SfoI GGC'GCC
Cuts at: 1826 2074 1826
Size: 248 4146
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
SmaI CCC'GGG
Cuts at: 1416 1416
Size: 4394
SmlI C'TyrA_G
Cuts at: 1549 2464 3178 3440 1549
Size: 915 714 262 2503
Fragments arranged by size:
2503 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4394
SphI G_CATG'C
Cuts at: 1447 1947 1447
Size: 500 3894
StuI AGG'CCT
Cuts at: 490 490
Size: 4394
StyI C'CwwG_G
Cuts at: 107 877 1101 1528 1882 107
Size: 770 224 427 354 2619
Fragments arranged by size:
2619 770 427 354 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 127 133 340 1977 2110 2569 2778 127
Size: 6 207 1637 133 459 209 1743
Fragments arranged by size:
1743 1637 459 209 207 133 6
TatI w'GTAC_w
Cuts at: 3909 3909
Size: 4394
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3911 3943 3911
Size: 32 4362
Tth111I GACn'n_nGTC
Cuts at: 2191 2191
Size: 4394
XbaI T'CTAG_A
Cuts at: 1425 1425
Size: 4394
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1889 1889
Size: 4394
XmaI C'CCGG_G
Cuts at: 1414 1414
Size: 4394
XmnI GAAnn'nnTTC
Cuts at: 1096 1291 1096
Size: 195 4199
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PasI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AlfI ApaI
AscI BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsiWI BsmBI BspEI BsrGI BssHII BstEII
BstZ17I Bsu36I CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NheI NotI NruI NsiI PacI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI