Restriction map for
pF1KA0129
(Circular) (Six-base) MAPSORT of: pF1KA0129.seq Check: 879 from: 1 to: 4439
pF1KA0129 4439 bp
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1355 1355
Size: 4439
AccI GT'mk_AC
Cuts at: 1477 1477
Size: 4439
Acc65I G'GTAC_C
Cuts at: 850 1455 850
Size: 605 3834
AclI AA'CG_TT
Cuts at: 4279 4279
Size: 4439
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 625 2264 2696 3443 3711 460
Size: 165 1639 432 747 268 1188
Fragments arranged by size:
1639 1188 747 432 268 165
AfeI AGC'GCT
Cuts at: 488 1204 488
Size: 716 3723
AflII C'TTAA_G
Cuts at: 799 799
Size: 4439
AflIII A'CryG_T
Cuts at: 1791 2895 3872 1791
Size: 1104 977 2358
Fragments arranged by size:
2358 1104 977
AgeI A'CCGG_T
Cuts at: 2827 2827
Size: 4439
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1267 1267
Size: 4439
AlwNI CAG_nnn'CTG
Cuts at: 602 1744 3311 602
Size: 1142 1567 1730
Fragments arranged by size:
1730 1567 1142
ApaI G_GGCC'C
Cuts at: 1004 1004
Size: 4439
ApaLI G'TGCA_C
Cuts at: 304 3209 304
Size: 2905 1534
ApoI r'AATT_y
Cuts at: 448 499 1443 3792 4048 448
Size: 51 944 2349 256 839
Fragments arranged by size:
2349 944 839 256 51
AseI AT'TA_AT
Cuts at: 20 20
Size: 4439
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4439
AvaI C'yCGr_G
Cuts at: 688 1459 688
Size: 771 3668
BamHI G'GATC_C
Cuts at: 921 1464 921
Size: 543 3896
BanI G'GyrC_C
Cuts at: 850 1102 1142 1193 1455 1869 2117 2152 850
Size: 252 40 51 262 414 248 35 3137
Fragments arranged by size:
3137 414 262 252 248 51 40 35
BanII G_rGCy'C
Cuts at: 209 623 728 1004 1453 2483 209
Size: 414 105 276 449 1030 2165
Fragments arranged by size:
2165 1030 449 414 276 105
BbeI G_GCGC'C
Cuts at: 1106 1146 1873 2121 1106
Size: 40 727 248 3424
Fragments arranged by size:
3424 727 248 40
BbsI GAAGACnn'nnnn_
Cuts at: 1278 1278
Size: 4439
BciVI GTATCCnnnnn_n'
Cuts at: 2337 3098 4439 2337
Size: 761 1341 2337
Fragments arranged by size:
2337 1341 761
BclI T'GATC_A
Cuts at: 1959 1959
Size: 4439
BglI GCCn_nnn'nGGC
Cuts at: 989 989
Size: 4439
BglII A'GATC_T
Cuts at: 1954 3991 1954
Size: 2037 2402
BlpI GC'TnA_GC
Cuts at: 203 1551 203
Size: 1348 3091
Bme1580I G_kGCm'C
Cuts at: 308 713 1004 1010 2064 2157 3213 308
Size: 405 291 6 1054 93 1056 1534
Fragments arranged by size:
1534 1056 1054 405 291 93 6
BmgBI CAC'GTC
Cuts at: 1089 1326 1089
Size: 237 4202
BmrI ACTGGGnnnn_n'
Cuts at: 1086 1230 1651 1732 2067 4211 1086
Size: 144 421 81 335 2144 1314
Fragments arranged by size:
2144 1314 421 335 144 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2806 2806
Size: 4439
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 514 624 313
Size: 201 110 4128
Fragments arranged by size:
4128 201 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 1183 1234 1615 2494 2986 3284 391
Size: 792 51 381 879 492 298 1546
Fragments arranged by size:
1546 879 792 492 381 298 51
BsaI GGTCTCn'nnnn_
Cuts at: 4143 4143
Size: 4439
BsaAI yAC'GTr
Cuts at: 309 1792 309
Size: 1483 2956
BsaBI GATnn'nnATC
Cuts at: 6 1953 3990 6
Size: 1947 2037 455
Fragments arranged by size:
2037 1947 455
BsaHI Gr'CG_yC
Cuts at: 781 1103 1143 1283 1307 1870 2118 2820 4316
Size: 322 40 140 24 563 248 702 1496
Cuts at: 4316 781
Size: 904
Fragments arranged by size:
1496 904 702 563 322 248 140 40
24
BsaWI w'CCGG_w
Cuts at: 1628 2149 2827 3101 3248 3659 1628
Size: 521 678 274 147 411 2408
Fragments arranged by size:
2408 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 255 987 1864 2622 3199 3695 255
Size: 732 877 758 577 496 999
Fragments arranged by size:
999 877 758 732 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 1111 331
Size: 780 3659
BsiEI CG_ry'CG
Cuts at: 104 125 195 963 1068 1160 1275 1981 2027
Size: 21 70 768 105 92 115 706 46
Cuts at: 2027 3235 104
Size: 1208 1308
Fragments arranged by size:
1308 1208 768 706 115 105 92 70
46 21
BsiHKAI G_wGCw'C
Cuts at: 209 308 1453 2231 2421 3213 209
Size: 99 1145 778 190 792 1435
Fragments arranged by size:
1435 1145 792 778 190 99
Bsp1286I G_dGCh'C
Cuts at: 209 308 623 713 728 1004 1010 1453 2064
Size: 99 315 90 15 276 6 443 611
Cuts at: 2064 2157 2231 2421 2483 3213 209
Size: 93 74 190 62 730 1435
Fragments arranged by size:
1435 730 611 443 315 276 190 99
93 90 74 62 15 6
BspEI T'CCGG_A
Cuts at: 3659 3659
Size: 4439
BspHI T'CATG_A
Cuts at: 578 3615 578
Size: 3037 1402
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1355 1489 1703 2005 2386 1355
Size: 134 214 302 381 3408
Fragments arranged by size:
3408 381 302 214 134
BsrBI CCG'CTC
Cuts at: 50 1380 2731 2785 4269 4434 50
Size: 1330 1351 54 1484 165 55
Fragments arranged by size:
1484 1351 1330 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2351 2351
Size: 4439
BsrFI r'CCGG_y
Cuts at: 1063 1105 1274 1303 2437 2618 2827 1063
Size: 42 169 29 1134 181 209 2675
Fragments arranged by size:
2675 1134 209 181 169 42 29
BsrGI T'GTAC_A
Cuts at: 536 536
Size: 4439
BssHII G'CGCG_C
Cuts at: 239 260 904 940 239
Size: 21 644 36 3738
Fragments arranged by size:
3738 644 36 21
BssSI C'ACGA_G
Cuts at: 751 1046 2710 3068 751
Size: 295 1664 358 2122
Fragments arranged by size:
2122 1664 358 295
BstAPI GCAn_nnn'nTGC
Cuts at: 669 815 1816 669
Size: 146 1001 3292
Fragments arranged by size:
3292 1001 146
BstBI TT'CG_AA
Cuts at: 2800 2800
Size: 4439
BstYI r'GATC_y
Cuts at: 1 921 1464 1931 1954 2289 2535 3547 3633
Size: 920 543 467 23 335 246 1012 86
Cuts at: 3633 3645 3991 1
Size: 12 346 449
Fragments arranged by size:
1012 920 543 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 267 761 1146 107
Size: 160 494 385 3400
Fragments arranged by size:
3400 494 385 160
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2389 2570 2733 2389
Size: 181 163 4095
Fragments arranged by size:
4095 181 163
BtsI GCAGTG_nn'
Cuts at: 1682 1682
Size: 4439
DraI TTT'AAA
Cuts at: 335 1438 335
Size: 1103 3336
DraIII CAC_nnn'GTG
Cuts at: 279 279
Size: 4439
DrdI GACnn_nn'nnGTC
Cuts at: 2145 3003 2145
Size: 858 3581
EaeI y'GGCC_r
Cuts at: 270 491 653 1065 1157 1236 2024 2198 2589
Size: 221 162 412 92 79 788 174 391
Cuts at: 2589 2616 3961 270
Size: 27 1345 748
Fragments arranged by size:
1345 788 748 412 391 221 174 162
92 79 27
EagI C'GGCC_G
Cuts at: 1065 1157 2024 1065
Size: 92 867 3480
Fragments arranged by size:
3480 867 92
EarI CTCTTCn'nnn_
Cuts at: 214 473 2462 2672 214
Size: 259 1989 210 1981
Fragments arranged by size:
1989 1981 259 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 945 2647 2957 3103 4249 945
Size: 1702 310 146 1146 1135
Fragments arranged by size:
1702 1146 1135 310 146
EcoICRI GAG'CTC
Cuts at: 207 1451 207
Size: 1244 3195
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 460 514 624 625 2264 2696 3443 3711
Size: 147 54 110 1 1639 432 747 268
Cuts at: 3711 313
Size: 1041
Fragments arranged by size:
1639 1041 747 432 268 147 110 54
1
EcoO109I rG'GnC_Cy
Cuts at: 747 1000 1151 1578 747
Size: 253 151 427 3608
Fragments arranged by size:
3608 427 253 151
EcoRI G'AATT_C
Cuts at: 1443 1443
Size: 4439
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 756 2458 756
Size: 1702 2737
FspI TGC'GCA
Cuts at: 2220 2220
Size: 4439
HaeII r_GCGC'y
Cuts at: 177 292 490 1106 1146 1206 1873 2121 3143
Size: 115 198 616 40 60 667 248 1022
Cuts at: 3143 4122 177
Size: 979 494
Fragments arranged by size:
1022 979 667 616 494 248 198 115
60 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2281 2311 2313 2343 2281
Size: 30 2 30 4377
Fragments arranged by size:
4377 30 30 2
HincII GTy'rAC
Cuts at: 1086 1478 1086
Size: 392 4047
KasI G'GCGC_C
Cuts at: 1102 1142 1869 2117 1102
Size: 40 727 248 3424
Fragments arranged by size:
3424 727 248 40
KpnI G_GTAC'C
Cuts at: 854 1459 854
Size: 605 3834
MluI A'CGCG_T
Cuts at: 3872 3872
Size: 4439
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 128 617 3110 3294 128
Size: 489 2493 184 1273
Fragments arranged by size:
2493 1273 489 184
MscI TGG'CCA
Cuts at: 272 493 2200 272
Size: 221 1707 2511
Fragments arranged by size:
2511 1707 221
MslI CAynn'nnrTG
Cuts at: 2428 2555 2428
Size: 127 4312
MspA1I CmG'CkG
Cuts at: 221 230 301 342 1148 1550 1864 2224 3237
Size: 9 71 41 806 402 314 360 1013
Cuts at: 3237 3482 221
Size: 245 1178
Fragments arranged by size:
1178 1013 806 402 360 314 245 71
41 9
NaeI GCC'GGC
Cuts at: 1107 1305 2620 1107
Size: 198 1315 2926
Fragments arranged by size:
2926 1315 198
NarI GG'CG_CC
Cuts at: 1103 1143 1870 2118 1103
Size: 40 727 248 3424
Fragments arranged by size:
3424 727 248 40
NcoI C'CATG_G
Cuts at: 107 107
Size: 4439
NgoMIV G'CCGG_C
Cuts at: 1105 1303 2618 1105
Size: 198 1315 2926
Fragments arranged by size:
2926 1315 198
NspI r_CATG'y
Cuts at: 1492 1992 2899 1492
Size: 500 907 3032
Fragments arranged by size:
3032 907 500
PasI CC'CwG_GG
Cuts at: 139 139
Size: 4439
PciI A'CATG_T
Cuts at: 2895 2895
Size: 4439
PflMI CCAn_nnn'nTGG
Cuts at: 279 279
Size: 4439
PfoI T'CCnGG_A
Cuts at: 1289 1370 1289
Size: 81 4358
PmeI GTTT'AAAC
Cuts at: 1438 1438
Size: 4439
PmlI CAC'GTG
Cuts at: 309 309
Size: 4439
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3578 3610 3578
Size: 32 4407
PspOMI G'GGCC_C
Cuts at: 1000 1000
Size: 4439
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 449 481 449
Size: 32 4407
PstI C_TGCA'G
Cuts at: 1486 2171 1486
Size: 685 3754
PvuI CG_AT'CG
Cuts at: 104 963 104
Size: 859 3580
PvuII CAG'CTG
Cuts at: 342 1864 2224 342
Size: 1522 360 2557
Fragments arranged by size:
2557 1522 360
RsrII CG'GwC_CG
Cuts at: 2634 2634
Size: 4439
SacI G_AGCT'C
Cuts at: 209 1453 209
Size: 1244 3195
SacII CC_GC'GG
Cuts at: 1149 1149
Size: 4439
SalI G'TCGA_C
Cuts at: 1476 1476
Size: 4439
SapI GCTCTTCn'nnn_
Cuts at: 214 2462 2672 214
Size: 2248 210 1981
Fragments arranged by size:
2248 1981 210
SbfI CC_TGCA'GG
Cuts at: 1486 1486
Size: 4439
ScaI AGT'ACT
Cuts at: 1219 3956 1219
Size: 2737 1702
SfcI C'TryA_G
Cuts at: 33 1482 2167 3160 3351 33
Size: 1449 685 993 191 1121
Fragments arranged by size:
1449 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1104 1144 1871 2119 1104
Size: 40 727 248 3424
Fragments arranged by size:
3424 727 248 40
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4439
SmaI CCC'GGG
Cuts at: 1461 1461
Size: 4439
SmlI C'TyrA_G
Cuts at: 406 799 1198 1213 1594 2509 3001 3263 406
Size: 393 399 15 381 915 492 262 1582
Fragments arranged by size:
1582 915 492 399 393 381 262 15
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4439
SphI G_CATG'C
Cuts at: 1492 1992 1492
Size: 500 3939
StyI C'CwwG_G
Cuts at: 107 1573 1927 107
Size: 1466 354 2619
Fragments arranged by size:
2619 1466 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1104 2022 2155 2614 2823 1104
Size: 918 133 459 209 2720
Fragments arranged by size:
2720 918 459 209 133
TatI w'GTAC_w
Cuts at: 536 769 1217 3954 536
Size: 233 448 2737 1021
Fragments arranged by size:
2737 1021 448 233
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 906 938 3956 3988 906
Size: 32 3018 32 1357
Fragments arranged by size:
3018 1357 32 32
Tth111I GACn'n_nGTC
Cuts at: 2236 2236
Size: 4439
XbaI T'CTAG_A
Cuts at: 1470 1470
Size: 4439
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1934 1934
Size: 4439
XmaI C'CCGG_G
Cuts at: 1459 1459
Size: 4439
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AhdI AleI AloI AscI AvrII BaeI BbvCI
BcgI BfrBI BmtI Bpu10I BsaXI BseRI BsiWI BsmI
BsmBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PpuMI PshAI PsiI PspXI
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI