Restriction map for
pF1KA0130
(Circular) (Six-base) MAPSORT of: pF1KA0130.seq Check: 4221 from: 1 to: 6080
pF1KA0130 6080 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AccI GT'mk_AC
Cuts at: 2279 2330 3118 2279
Size: 51 788 5241
Fragments arranged by size:
5241 788 51
Acc65I G'GTAC_C
Cuts at: 3096 3096
Size: 6080
AclI AA'CG_TT
Cuts at: 5920 5920
Size: 6080
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 319 577 1153 2386 3905 4337 5084 5352
Size: 174 258 576 1233 1519 432 747 268
Cuts at: 5352 145
Size: 873
Fragments arranged by size:
1519 1233 873 747 576 432 268 258
174
AfeI AGC'GCT
Cuts at: 157 1168 157
Size: 1011 5069
AflIII A'CryG_T
Cuts at: 413 3432 4536 5513 413
Size: 3019 1104 977 980
Fragments arranged by size:
3019 1104 980 977
AgeI A'CCGG_T
Cuts at: 4468 4468
Size: 6080
AleI CACnn'nnGTG
Cuts at: 1765 2179 2959 1765
Size: 414 780 4886
Fragments arranged by size:
4886 780 414
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 471 2953 471
Size: 2482 3598
AlwNI CAG_nnn'CTG
Cuts at: 520 565 600 894 1666 3385 4952 520
Size: 45 35 294 772 1719 1567 1648
Fragments arranged by size:
1719 1648 1567 772 294 45 35
ApaI G_GGCC'C
Cuts at: 636 1578 636
Size: 942 5138
ApaLI G'TGCA_C
Cuts at: 4850 4850
Size: 6080
ApoI r'AATT_y
Cuts at: 193 1507 1525 3084 5433 5689 193
Size: 1314 18 1559 2349 256 584
Fragments arranged by size:
2349 1559 1314 584 256 18
AseI AT'TA_AT
Cuts at: 20 20
Size: 6080
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6080
AvaI C'yCGr_G
Cuts at: 374 856 997 2520 3100 374
Size: 482 141 1523 580 3354
Fragments arranged by size:
3354 1523 580 482 141
BamHI G'GATC_C
Cuts at: 3105 3105
Size: 6080
BanI G'GyrC_C
Cuts at: 763 862 1671 3096 3510 3758 3793 763
Size: 99 809 1425 414 248 35 3050
Fragments arranged by size:
3050 1425 809 414 248 99 35
BanII G_rGCy'C
Cuts at: 477 636 986 1016 1352 1578 2695 2728 3094
Size: 159 350 30 336 226 1117 33 366
Cuts at: 3094 4124 477
Size: 1030 2433
Fragments arranged by size:
2433 1117 1030 366 350 336 226 159
33 30
BbeI G_GCGC'C
Cuts at: 3514 3762 3514
Size: 248 5832
BbsI GAAGACnn'nnnn_
Cuts at: 2627 2627
Size: 6080
BbvCI CC'TCA_GC
Cuts at: 613 1667 3025 613
Size: 1054 1358 3668
Fragments arranged by size:
3668 1358 1054
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2661 2695 2661
Size: 34 6046
BciVI GTATCCnnnnn_n'
Cuts at: 3978 4739 6080 3978
Size: 761 1341 3978
Fragments arranged by size:
3978 1341 761
BclI T'GATC_A
Cuts at: 2099 3600 2099
Size: 1501 4579
BfrBI ATG'CAT
Cuts at: 458 458
Size: 6080
BglI GCCn_nnn'nGGC
Cuts at: 2542 2542
Size: 6080
BglII A'GATC_T
Cuts at: 959 3595 5632 959
Size: 2636 2037 1407
Fragments arranged by size:
2636 2037 1407
BlpI GC'TnA_GC
Cuts at: 1256 2689 3192 1256
Size: 1433 503 4144
Fragments arranged by size:
4144 1433 503
Bme1580I G_kGCm'C
Cuts at: 636 865 1578 1676 2527 2559 3705 3798 4854
Size: 229 713 98 851 32 1146 93 1056
Cuts at: 4854 636
Size: 1862
Fragments arranged by size:
1862 1146 1056 851 713 229 98 93
32
BmgBI CAC'GTC
Cuts at: 1989 2136 1989
Size: 147 5933
BmrI ACTGGGnnnn_n'
Cuts at: 2865 3292 3373 3708 5852 2865
Size: 427 81 335 2144 3093
Fragments arranged by size:
3093 2144 427 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 681 4447 681
Size: 3766 2314
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 180 542 565 622 976 2137 2284 2932 180
Size: 362 23 57 354 1161 147 648 3328
Fragments arranged by size:
3328 1161 648 362 354 147 57 23
Bpu10I CC'TnA_GC
Cuts at: 613 1667 3025 613
Size: 1054 1358 3668
Fragments arranged by size:
3668 1358 1054
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1000 1024 1354 3256 4135 4627 4925 1000
Size: 24 330 1902 879 492 298 2155
Fragments arranged by size:
2155 1902 879 492 330 298 24
BsaI GGTCTCn'nnnn_
Cuts at: 1678 2852 5784 1678
Size: 1174 2932 1974
Fragments arranged by size:
2932 1974 1174
BsaAI yAC'GTr
Cuts at: 3433 3433
Size: 6080
BsaBI GATnn'nnATC
Cuts at: 6 3594 5631 6
Size: 3588 2037 455
Fragments arranged by size:
3588 2037 455
BsaHI Gr'CG_yC
Cuts at: 2327 2590 3511 3759 4461 5957 2327
Size: 263 921 248 702 1496 2450
Fragments arranged by size:
2450 1496 921 702 263 248
BsaWI w'CCGG_w
Cuts at: 740 1401 1991 3269 3790 4468 4742 4889 5300
Size: 661 590 1278 521 678 274 147 411
Cuts at: 5300 740
Size: 1520
Fragments arranged by size:
1520 1278 678 661 590 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1003 1033 1655 1685 2110 2140 2272 2302 1003
Size: 30 622 30 425 30 132 30 4781
Fragments arranged by size:
4781 622 425 132 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 673 1024 1726 1788 2481 2562 2823 2908 673
Size: 351 702 62 693 81 261 85 3845
Fragments arranged by size:
3845 702 693 351 261 85 81 62
BseYI C'CCAG_C
Cuts at: 565 1286 1413 1998 2273 2457 3505 4263 4840
Size: 721 127 585 275 184 1048 758 577
Cuts at: 4840 5336 565
Size: 496 1309
Fragments arranged by size:
1309 1048 758 721 585 577 496 275
184 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 492 625 1837 2299 2683 2899 3053 492
Size: 133 1212 462 384 216 154 3519
Fragments arranged by size:
3519 1212 462 384 216 154 133
BsiEI CG_ry'CG
Cuts at: 104 3622 3668 4876 104
Size: 3518 46 1208 1308
Fragments arranged by size:
3518 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1016 1280 2387 2695 3094 3872 4062 4854 1016
Size: 264 1107 308 399 778 190 792 2242
Fragments arranged by size:
2242 1107 792 778 399 308 264 190
BsmI GAATG_Cn'
Cuts at: 460 687 1311 460
Size: 227 624 5229
Fragments arranged by size:
5229 624 227
BsmBI CGTCTCn'nnnn_
Cuts at: 877 2365 877
Size: 1488 4592
Bsp1286I G_dGCh'C
Cuts at: 477 636 865 986 1016 1280 1352 1578 1676
Size: 159 229 121 30 264 72 226 98
Cuts at: 1676 2387 2527 2559 2695 2728 3094 3705 3798
Size: 711 140 32 136 33 366 611 93
Cuts at: 3798 3872 4062 4124 4854 477
Size: 74 190 62 730 1703
Fragments arranged by size:
1703 730 711 611 366 264 229 226
190 159 140 136 121 98 93 74
72 62 33 32 30
BspEI T'CCGG_A
Cuts at: 740 1991 5300 740
Size: 1251 3309 1520
Fragments arranged by size:
3309 1520 1251
BspHI T'CATG_A
Cuts at: 2102 5256 2102
Size: 3154 2926
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1708 2213 3130 3344 3646 4027 1708
Size: 505 917 214 302 381 3761
Fragments arranged by size:
3761 917 505 381 302 214
BsrBI CCG'CTC
Cuts at: 50 528 2288 4372 4426 5910 6075 50
Size: 478 1760 2084 54 1484 165 55
Fragments arranged by size:
2084 1760 1484 478 165 55 54
BsrDI GCAATG_nn'
Cuts at: 394 3062 3992 394
Size: 2668 930 2482
Fragments arranged by size:
2668 2482 930
BsrFI r'CCGG_y
Cuts at: 550 823 1560 4078 4259 4468 550
Size: 273 737 2518 181 209 2162
Fragments arranged by size:
2518 2162 737 273 209 181
BsrGI T'GTAC_A
Cuts at: 1183 1183
Size: 6080
BssSI C'ACGA_G
Cuts at: 4351 4709 4351
Size: 358 5722
BstAPI GCAn_nnn'nTGC
Cuts at: 520 2391 2946 3457 520
Size: 1871 555 511 3143
Fragments arranged by size:
3143 1871 555 511
BstBI TT'CG_AA
Cuts at: 2671 4441 2671
Size: 1770 4310
BstXI CCAn_nnnn'nTGG
Cuts at: 114 1207 1627 1767 2181 2262 114
Size: 1093 420 140 414 81 3932
Fragments arranged by size:
3932 1093 420 414 140 81
BstYI r'GATC_y
Cuts at: 1 710 959 1721 3105 3572 3595 3930 4176
Size: 709 249 762 1384 467 23 335 246
Cuts at: 4176 5188 5274 5286 5632 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1384 1012 762 709 467 449 346 335
249 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1709 1709
Size: 6080
BtgI C'CryG_G
Cuts at: 2925 2925
Size: 6080
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 630 4030 4211 4374 630
Size: 3400 181 163 2336
Fragments arranged by size:
3400 2336 181 163
BtsI GCAGTG_nn'
Cuts at: 763 2405 2525 2887 3323 763
Size: 1642 120 362 436 3520
Fragments arranged by size:
3520 1642 436 362 120
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1037 1072 1037
Size: 35 6045
DraI TTT'AAA
Cuts at: 3079 3079
Size: 6080
DraIII CAC_nnn'GTG
Cuts at: 846 846
Size: 6080
DrdI GACnn_nn'nnGTC
Cuts at: 385 3786 4644 385
Size: 3401 858 1821
Fragments arranged by size:
3401 1821 858
EaeI y'GGCC_r
Cuts at: 2543 2810 2922 3011 3665 3839 4230 4257 5602
Size: 267 112 89 654 174 391 27 1345
Cuts at: 5602 2543
Size: 3021
Fragments arranged by size:
3021 1345 654 391 267 174 112 89
27
EagI C'GGCC_G
Cuts at: 3665 3665
Size: 6080
EarI CTCTTCn'nnn_
Cuts at: 339 672 1438 2602 2650 4103 4313 339
Size: 333 766 1164 48 1453 210 2106
Fragments arranged by size:
2106 1453 1164 766 333 210 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1360 1738 2026 4288 4598 4744 5890 1360
Size: 378 288 2262 310 146 1146 1550
Fragments arranged by size:
2262 1550 1146 378 310 288 146
EcoICRI GAG'CTC
Cuts at: 1014 2693 3092 1014
Size: 1679 399 4002
Fragments arranged by size:
4002 1679 399
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 180 319 542 565 577 622 976 1153
Size: 35 139 223 23 12 45 354 177
Cuts at: 1153 2137 2284 2386 2932 3905 4337 5084 5352
Size: 984 147 102 546 973 432 747 268
Cuts at: 5352 145
Size: 873
Fragments arranged by size:
984 973 873 747 546 432 354 268
223 177 147 139 102 45 35 23
12
EcoNI CCTnn'n_nnAGG
Cuts at: 856 2353 2914 856
Size: 1497 561 4022
Fragments arranged by size:
4022 1497 561
EcoO109I rG'GnC_Cy
Cuts at: 378 633 1575 1893 2790 3219 378
Size: 255 942 318 897 429 3239
Fragments arranged by size:
3239 942 897 429 318 255
EcoRI G'AATT_C
Cuts at: 1525 3084 1525
Size: 1559 4521
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 213 843 4099 213
Size: 630 3256 2194
Fragments arranged by size:
3256 2194 630
FspI TGC'GCA
Cuts at: 3861 3861
Size: 6080
HaeII r_GCGC'y
Cuts at: 159 488 600 1170 3514 3762 4784 5763 159
Size: 329 112 570 2344 248 1022 979 476
Fragments arranged by size:
2344 1022 979 570 476 329 248 112
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 874 906 2272 2304 3922 3952 3954 3984 874
Size: 32 1366 32 1618 30 2 30 2970
Fragments arranged by size:
2970 1618 1366 32 32 30 30 2
HincII GTy'rAC
Cuts at: 120 1110 1242 2781 3119 120
Size: 990 132 1539 338 3081
Fragments arranged by size:
3081 1539 990 338 132
HindIII A'AGCT_T
Cuts at: 968 968
Size: 6080
KasI G'GCGC_C
Cuts at: 3510 3758 3510
Size: 248 5832
KpnI G_GTAC'C
Cuts at: 3100 3100
Size: 6080
MluI A'CGCG_T
Cuts at: 5513 5513
Size: 6080
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1130 2945 4751 4935 1130
Size: 1815 1806 184 2275
Fragments arranged by size:
2275 1815 1806 184
MscI TGG'CCA
Cuts at: 2812 3013 3841 2812
Size: 201 828 5051
Fragments arranged by size:
5051 828 201
MslI CAynn'nnrTG
Cuts at: 112 412 1765 2179 2959 4069 4196 112
Size: 300 1353 414 780 1110 127 1996
Fragments arranged by size:
1996 1353 1110 780 414 300 127
MspA1I CmG'CkG
Cuts at: 565 906 1332 2043 2857 3191 3505 3865 4878
Size: 341 426 711 814 334 314 360 1013
Cuts at: 4878 5123 565
Size: 245 1522
Fragments arranged by size:
1522 1013 814 711 426 360 341 334
314 245
NaeI GCC'GGC
Cuts at: 552 4261 552
Size: 3709 2371
NarI GG'CG_CC
Cuts at: 3511 3759 3511
Size: 248 5832
NgoMIV G'CCGG_C
Cuts at: 550 4259 550
Size: 3709 2371
NsiI A_TGCA'T
Cuts at: 460 460
Size: 6080
NspI r_CATG'y
Cuts at: 417 805 927 1434 1707 2127 2436 2787 3133
Size: 388 122 507 273 420 309 351 346
Cuts at: 3133 3633 4540 417
Size: 500 907 1957
Fragments arranged by size:
1957 907 507 500 420 388 351 346
309 273 122
PasI CC'CwG_GG
Cuts at: 3036 3036
Size: 6080
PciI A'CATG_T
Cuts at: 413 4536 413
Size: 4123 1957
PflMI CCAn_nnn'nTGG
Cuts at: 565 565
Size: 6080
PfoI T'CCnGG_A
Cuts at: 953 1438 2114 953
Size: 485 676 4919
Fragments arranged by size:
4919 676 485
PmeI GTTT'AAAC
Cuts at: 3079 3079
Size: 6080
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5219 5251 5219
Size: 32 6048
PpuMI rG'GwC_Cy
Cuts at: 378 1893 2790 378
Size: 1515 897 3668
Fragments arranged by size:
3668 1515 897
PshAI GACnn'nnGTC
Cuts at: 2449 2589 2449
Size: 140 5940
PspOMI G'GGCC_C
Cuts at: 632 1574 632
Size: 942 5138
PspXI vC'TCGA_Gb
Cuts at: 856 856
Size: 6080
PstI C_TGCA'G
Cuts at: 525 2030 2078 2144 2942 3127 3812 525
Size: 1505 48 66 798 185 685 2793
Fragments arranged by size:
2793 1505 798 685 185 66 48
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6080
PvuII CAG'CTG
Cuts at: 906 1332 2043 3505 3865 906
Size: 426 711 1462 360 3121
Fragments arranged by size:
3121 1462 711 426 360
RsrII CG'GwC_CG
Cuts at: 1269 4275 1269
Size: 3006 3074
SacI G_AGCT'C
Cuts at: 1016 2695 3094 1016
Size: 1679 399 4002
Fragments arranged by size:
4002 1679 399
SalI G'TCGA_C
Cuts at: 3117 3117
Size: 6080
SanDI GG'GwC_CC
Cuts at: 2790 2790
Size: 6080
SapI GCTCTTCn'nnn_
Cuts at: 4103 4313 4103
Size: 210 5870
SbfI CC_TGCA'GG
Cuts at: 3127 3127
Size: 6080
ScaI AGT'ACT
Cuts at: 1075 5597 1075
Size: 4522 1558
SfcI C'TryA_G
Cuts at: 33 521 2026 2074 2140 2938 3123 3808 4801
Size: 488 1505 48 66 798 185 685 993
Cuts at: 4801 4992 33
Size: 191 1121
Fragments arranged by size:
1505 1121 993 798 685 488 191 185
66 48
SfoI GGC'GCC
Cuts at: 3512 3760 3512
Size: 248 5832
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6080
SmaI CCC'GGG
Cuts at: 2522 3102 2522
Size: 580 5500
SmlI C'TyrA_G
Cuts at: 856 1015 1039 1369 3235 4150 4642 4904 856
Size: 159 24 330 1866 915 492 262 2032
Fragments arranged by size:
2032 1866 915 492 330 262 159 24
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6080
SphI G_CATG'C
Cuts at: 805 927 1707 3133 3633 805
Size: 122 780 1426 500 3252
Fragments arranged by size:
3252 1426 780 500 122
StuI AGG'CCT
Cuts at: 664 1837 664
Size: 1173 4907
StyI C'CwwG_G
Cuts at: 2255 2999 3214 3568 2255
Size: 744 215 354 4767
Fragments arranged by size:
4767 744 354 215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1286 2750 2884 3663 3796 4255 4464 1286
Size: 1464 134 779 133 459 209 2902
Fragments arranged by size:
2902 1464 779 459 209 134 133
TatI w'GTAC_w
Cuts at: 1073 1183 5595 1073
Size: 110 4412 1558
Fragments arranged by size:
4412 1558 110
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 935 967 1003 1035 1420 1452 1655 1687 2110
Size: 32 36 32 385 32 203 32 423
Cuts at: 2110 2142 5597 5629 935
Size: 32 3455 32 1386
Fragments arranged by size:
3455 1386 423 385 203 36 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 888 3877 888
Size: 2989 3091
XbaI T'CTAG_A
Cuts at: 3111 3111
Size: 6080
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2424 2470 3000 3006 3575 2424
Size: 46 530 6 569 4929
Fragments arranged by size:
4929 569 530 46 6
XhoI C'TCGA_G
Cuts at: 856 856
Size: 6080
XmaI C'CCGG_G
Cuts at: 2520 3100 2520
Size: 580 5500
XmnI GAAnn'nnTTC
Cuts at: 193 1495 1525 193
Size: 1302 30 4748
Fragments arranged by size:
4748 1302 30
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AloI AscI AvrII BaeI
BmtI BsiWI BssHII BstEII BstZ17I ClaI EcoRV FseI
FspAI HpaI MfeI NcoI NdeI NheI NotI NruI
PacI PmlI PsiI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI ZraI