Restriction map for
pF1KA0133
(Circular) (Six-base) MAPSORT of: pF1KA0133.seq Check: 9408 from: 1 to: 7685
pF1KA0133 7685 bp
With 182 enzymes: SgfI *
August 18, 2008 14:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 513 716 777 1133 513
Size: 203 61 356 7065
Fragments arranged by size:
7065 356 203 61
AatII G_ACGT'C
Cuts at: 4565 4565
Size: 7685
AccI GT'mk_AC
Cuts at: 499 4723 499
Size: 4224 3461
Acc65I G'GTAC_C
Cuts at: 4701 4701
Size: 7685
AclI AA'CG_TT
Cuts at: 7525 7525
Size: 7685
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 376 1363 1388 2207 4131 4201 5510 5942
Size: 63 987 25 819 1924 70 1309 432
Cuts at: 5942 6295 6957 313
Size: 353 662 1041
Fragments arranged by size:
1924 1309 1041 987 819 662 432 353
70 63 25
AflII C'TTAA_G
Cuts at: 136 136
Size: 7685
AflIII A'CryG_T
Cuts at: 1643 3881 4466 5037 6837 7118 1643
Size: 2238 585 571 1800 281 2210
Fragments arranged by size:
2238 2210 1800 585 571 281
AhdI GACnn_n'nnGTC
Cuts at: 1647 3871 1647
Size: 2224 5461
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 370 1718 1807 370
Size: 1348 89 6248
Fragments arranged by size:
6248 1348 89
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1948 1980 2716 2748 1948
Size: 32 736 32 6885
Fragments arranged by size:
6885 736 32 32
AlwNI CAG_nnn'CTG
Cuts at: 540 792 1552 1638 1854 3333 3397 3591 3745
Size: 252 760 86 216 1479 64 194 154
Cuts at: 3745 3867 4572 4990 6428 540
Size: 122 705 418 1438 1797
Fragments arranged by size:
1797 1479 1438 760 705 418 252 216
194 154 122 86 64
ApaI G_GGCC'C
Cuts at: 1588 1950 2006 3429 1588
Size: 362 56 1423 5844
Fragments arranged by size:
5844 1423 362 56
ApaLI G'TGCA_C
Cuts at: 4379 6523 4379
Size: 2144 5541
ApoI r'AATT_y
Cuts at: 1244 3094 3749 4689 7038 7294 1244
Size: 1850 655 940 2349 256 1635
Fragments arranged by size:
2349 1850 1635 940 655 256
AseI AT'TA_AT
Cuts at: 20 386 20
Size: 366 7319
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7685
AvaI C'yCGr_G
Cuts at: 1581 3428 4209 4705 1581
Size: 1847 781 496 4561
Fragments arranged by size:
4561 1847 781 496
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2122 2155 2122
Size: 33 7652
BamHI G'GATC_C
Cuts at: 448 4710 448
Size: 4262 3423
BanI G'GyrC_C
Cuts at: 2584 3437 4701 5115 5363 5398 2584
Size: 853 1264 414 248 35 4871
Fragments arranged by size:
4871 1264 853 414 248 35
BanII G_rGCy'C
Cuts at: 881 1588 1844 1950 2006 3429 4619 4699 5729
Size: 707 256 106 56 1423 1190 80 1030
Cuts at: 5729 881
Size: 2837
Fragments arranged by size:
2837 1423 1190 1030 707 256 106 80
56
BbeI G_GCGC'C
Cuts at: 3441 5119 5367 3441
Size: 1678 248 5759
Fragments arranged by size:
5759 1678 248
BbsI GAAGACnn'nnnn_
Cuts at: 913 1351 1467 4197 913
Size: 438 116 2730 4401
Fragments arranged by size:
4401 2730 438 116
BbvCI CC'TCA_GC
Cuts at: 781 1553 3928 4424 781
Size: 772 2375 496 4042
Fragments arranged by size:
4042 2375 772 496
BciVI GTATCCnnnnn_n'
Cuts at: 2380 5583 6639 7685 2380
Size: 3203 1056 1046 2380
Fragments arranged by size:
3203 2380 1056 1046
BclI T'GATC_A
Cuts at: 3621 5205 3621
Size: 1584 6101
BglI GCCn_nnn'nGGC
Cuts at: 1456 1711 1456
Size: 255 7430
BglII A'GATC_T
Cuts at: 2027 5200 7237 2027
Size: 3173 2037 2475
Fragments arranged by size:
3173 2475 2037
BlpI GC'TnA_GC
Cuts at: 556 793 1289 3157 4797 556
Size: 237 496 1868 1640 3444
Fragments arranged by size:
3444 1868 1640 496 237
Bme1580I G_kGCm'C
Cuts at: 754 1588 1950 2006 2589 3429 4383 5310 5403
Size: 834 362 56 583 840 954 927 93
Cuts at: 5403 6527 754
Size: 1124 1912
Fragments arranged by size:
1912 1124 954 927 840 834 583 362
93 56
BmgBI CAC'GTC
Cuts at: 682 4134 682
Size: 3452 4233
BmrI ACTGGGnnnn_n'
Cuts at: 603 4455 4897 4978 5313 7457 603
Size: 3852 442 81 335 2144 831
Fragments arranged by size:
3852 2144 831 442 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6052 6052
Size: 7685
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 697 1636 1681 2044 2550 2785 3844 4210 697
Size: 939 45 363 506 235 1059 366 4172
Fragments arranged by size:
4172 1059 939 506 366 363 235 45
Bpu10I CC'TnA_GC
Cuts at: 781 851 1553 3928 4424 781
Size: 70 702 2375 496 4042
Fragments arranged by size:
4042 2375 702 496 70
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 2908 3855 4618 4861 5740 6454 6752 352
Size: 2556 947 763 243 879 714 298 1285
Fragments arranged by size:
2556 1285 947 879 763 714 298 243
BsaI GGTCTCn'nnnn_
Cuts at: 7389 7389
Size: 7685
BsaAI yAC'GTr
Cuts at: 1284 1646 4467 5038 1284
Size: 362 2821 571 3931
Fragments arranged by size:
3931 2821 571 362
BsaBI GATnn'nnATC
Cuts at: 6 3142 5199 7236 6
Size: 3136 2057 2037 455
Fragments arranged by size:
3136 2057 2037 455
BsaHI Gr'CG_yC
Cuts at: 3438 4562 5116 5364 6066 7562 3438
Size: 1124 554 248 702 1496 3561
Fragments arranged by size:
3561 1496 1124 702 554 248
BsaWI w'CCGG_w
Cuts at: 2180 4874 5395 6073 6484 6631 6905 2180
Size: 2694 521 678 411 147 274 2960
Fragments arranged by size:
2960 2694 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 587 617 587
Size: 30 7655
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 355 848 1147 1782 2723 2898 2901 3139 4123
Size: 493 299 635 941 175 3 238 984
Cuts at: 4123 4441 355
Size: 318 3599
Fragments arranged by size:
3599 984 941 635 493 318 299 238
175 3
BseYI C'CCAG_C
Cuts at: 2588 3333 3348 5110 5868 6533 6941 2588
Size: 745 15 1762 758 665 408 3332
Fragments arranged by size:
3332 1762 758 745 665 408 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1001 1729 1867 3919 4073 4201 1001
Size: 728 138 2052 154 128 4485
Fragments arranged by size:
4485 2052 728 154 138 128
BsiEI CG_ry'CG
Cuts at: 104 4679 5227 5273 6503 104
Size: 4575 548 46 1230 1286
Fragments arranged by size:
4575 1286 1230 548 46
BsiHKAI G_wGCw'C
Cuts at: 1844 1928 2982 3170 4229 4383 4619 4699 5477
Size: 84 1054 188 1059 154 236 80 778
Cuts at: 5477 5667 6527 1844
Size: 190 860 3002
Fragments arranged by size:
3002 1059 1054 860 778 236 190 188
154 84 80
BsmI GAATG_Cn'
Cuts at: 2570 3190 2570
Size: 620 7065
Bsp1286I G_dGCh'C
Cuts at: 754 881 1588 1844 1928 1950 2006 2589 2982
Size: 127 707 256 84 22 56 583 393
Cuts at: 2982 3170 3429 4229 4383 4619 4699 5310 5403
Size: 188 259 800 154 236 80 611 93
Cuts at: 5403 5477 5667 5729 6527 754
Size: 74 190 62 798 1912
Fragments arranged by size:
1912 800 798 707 611 583 393 259
256 236 190 188 154 127 93 84
80 74 62 56 22
BspHI T'CATG_A
Cuts at: 6117 6117
Size: 7685
BspMI ACCTGCnnnn'nnnn_
Cuts at: 513 657 716 777 1133 1403 2150 3366 4367
Size: 144 59 61 356 270 747 1216 1001
Cuts at: 4367 4735 4949 5251 5632 513
Size: 368 214 302 381 2566
Fragments arranged by size:
2566 1216 1001 747 381 368 356 302
270 214 144 61 59
BsrBI CCG'CTC
Cuts at: 50 5977 6031 7515 7680 50
Size: 5927 54 1484 165 55
Fragments arranged by size:
5927 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 5597 5597
Size: 7685
BsrFI r'CCGG_y
Cuts at: 1323 5683 5864 1323
Size: 4360 181 3144
Fragments arranged by size:
4360 3144 181
BsrGI T'GTAC_A
Cuts at: 4400 4400
Size: 7685
BssSI C'ACGA_G
Cuts at: 1843 4418 5956 6664 1843
Size: 2575 1538 708 2864
Fragments arranged by size:
2864 2575 1538 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1572 1854 3381 4224 5062 1572
Size: 282 1527 843 838 4195
Fragments arranged by size:
4195 1527 843 838 282
BstBI TT'CG_AA
Cuts at: 1437 6046 1437
Size: 4609 3076
BstEII G'GTnAC_C
Cuts at: 4485 4485
Size: 7685
BstXI CCAn_nnnn'nTGG
Cuts at: 1017 1898 2953 1017
Size: 881 1055 5749
Fragments arranged by size:
5749 1055 881
BstYI r'GATC_y
Cuts at: 1 320 448 1634 2027 3741 4710 5177 5200
Size: 319 128 1186 393 1714 969 467 23
Cuts at: 5200 5535 5781 6087 6099 6185 7237 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1714 1186 1052 969 467 449 393 335
319 306 246 128 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 730 4253 730
Size: 3523 4162
BtgI C'CryG_G
Cuts at: 107 938 1507 1592 2420 2876 107
Size: 831 569 85 828 456 4916
Fragments arranged by size:
4916 831 828 569 456 85
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1215 2228 4395 5635 5816 5979 1215
Size: 1013 2167 1240 181 163 2921
Fragments arranged by size:
2921 2167 1240 1013 181 163
BtsI GCAGTG_nn'
Cuts at: 1746 3498 3856 3922 4090 4249 4928 1746
Size: 1752 358 66 168 159 679 4503
Fragments arranged by size:
4503 1752 679 358 168 159 66
ClaI AT'CG_AT
Cuts at: 1415 1415
Size: 7685
DraI TTT'AAA
Cuts at: 4684 4684
Size: 7685
DraIII CAC_nnn'GTG
Cuts at: 3955 4224 3955
Size: 269 7416
DrdI GACnn_nn'nnGTC
Cuts at: 5391 6735 5391
Size: 1344 6341
EaeI y'GGCC_r
Cuts at: 527 639 768 1007 1208 2280 2393 2879 4676
Size: 112 129 239 201 1072 113 486 1797
Cuts at: 4676 5270 5444 5835 5862 7207 527
Size: 594 174 391 27 1345 1005
Fragments arranged by size:
1797 1345 1072 1005 594 486 391 239
201 174 129 113 112 27
EagI C'GGCC_G
Cuts at: 4676 5270 4676
Size: 594 7091
EarI CTCTTCn'nnn_
Cuts at: 1250 1514 2447 3723 4234 5708 5918 1250
Size: 264 933 1276 511 1474 210 3017
Fragments arranged by size:
3017 1474 1276 933 511 264 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5893 6635 6781 7495 5893
Size: 742 146 714 6083
Fragments arranged by size:
6083 742 714 146
EcoICRI GAG'CTC
Cuts at: 1842 4617 4697 1842
Size: 2775 80 4830
Fragments arranged by size:
4830 2775 80
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 376 697 1363 1388 1636 1681 2044 2207
Size: 63 321 666 25 248 45 363 163
Cuts at: 2207 2550 2785 3844 4131 4201 4210 5510 5942
Size: 343 235 1059 287 70 9 1300 432
Cuts at: 5942 6295 6957 313
Size: 353 662 1041
Fragments arranged by size:
1300 1059 1041 666 662 432 363 353
343 321 287 248 235 163 70 63
45 25 9
EcoNI CCTnn'n_nnAGG
Cuts at: 1129 3568 1129
Size: 2439 5246
EcoO109I rG'GnC_Cy
Cuts at: 1585 2003 3310 3345 3425 4824 1585
Size: 418 1307 35 80 1399 4446
Fragments arranged by size:
4446 1399 1307 418 80 35
EcoRI G'AATT_C
Cuts at: 1244 3094 4689 1244
Size: 1850 1595 4240
Fragments arranged by size:
4240 1850 1595
EcoRV GAT'ATC
Cuts at: 1355 1355
Size: 7685
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 256 2509 2766 3177 4506 5704 256
Size: 2253 257 411 1329 1198 2237
Fragments arranged by size:
2253 2237 1329 1198 411 257
FspI TGC'GCA
Cuts at: 5466 5466
Size: 7685
HaeII r_GCGC'y
Cuts at: 1462 3441 5119 5367 6597 7368 1462
Size: 1979 1678 248 1230 771 1779
Fragments arranged by size:
1979 1779 1678 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 372 404 844 876 1527 1559 1633 1665 2506
Size: 32 440 32 651 32 74 32 841
Cuts at: 2506 2538 3063 3095 3302 3334 3857 3889 4479
Size: 32 525 32 207 32 523 32 590
Cuts at: 4479 4511 5527 5557 5559 5589 372
Size: 32 1016 30 2 30 2468
Fragments arranged by size:
2468 1016 841 651 590 525 523 440
207 74 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 500 672 4724 500
Size: 172 4052 3461
Fragments arranged by size:
4052 3461 172
HindIII A'AGCT_T
Cuts at: 139 283 2247 4028 139
Size: 144 1964 1781 3796
Fragments arranged by size:
3796 1964 1781 144
KasI G'GCGC_C
Cuts at: 3437 5115 5363 3437
Size: 1678 248 5759
Fragments arranged by size:
5759 1678 248
KpnI G_GTAC'C
Cuts at: 4705 4705
Size: 7685
MluI A'CGCG_T
Cuts at: 7118 7118
Size: 7685
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1521 2759 3575 4451 6444 6628 1521
Size: 1238 816 876 1993 184 2578
Fragments arranged by size:
2578 1993 1238 876 816 184
MscI TGG'CCA
Cuts at: 641 770 1009 1210 2282 2395 5446 641
Size: 129 239 201 1072 113 3051 2880
Fragments arranged by size:
3051 2880 1072 239 201 129 113
MslI CAynn'nnrTG
Cuts at: 2650 3148 3805 5674 5801 2650
Size: 498 657 1869 127 4534
Fragments arranged by size:
4534 1869 657 498 127
MspA1I CmG'CkG
Cuts at: 780 1337 1549 2136 2763 2823 3916 4188 4501
Size: 557 212 587 627 60 1093 272 313
Cuts at: 4501 4796 5110 5470 6254 6499 780
Size: 295 314 360 784 245 1966
Fragments arranged by size:
1966 1093 784 627 587 557 360 314
313 295 272 245 212 60
NaeI GCC'GGC
Cuts at: 5866 5866
Size: 7685
NarI GG'CG_CC
Cuts at: 3438 5116 5364 3438
Size: 1678 248 5759
Fragments arranged by size:
5759 1678 248
NcoI C'CATG_G
Cuts at: 107 938 2420 2876 107
Size: 831 1482 456 4916
Fragments arranged by size:
4916 1482 831 456
NgoMIV G'CCGG_C
Cuts at: 5864 5864
Size: 7685
NspI r_CATG'y
Cuts at: 1610 3885 4738 5238 6841 1610
Size: 2275 853 500 1603 2454
Fragments arranged by size:
2454 2275 1603 853 500
PasI CC'CwG_GG
Cuts at: 3258 3258
Size: 7685
PciI A'CATG_T
Cuts at: 3881 6837 3881
Size: 2956 4729
PflMI CCAn_nnn'nTGG
Cuts at: 3306 3867 3955 3306
Size: 561 88 7036
Fragments arranged by size:
7036 561 88
PfoI T'CCnGG_A
Cuts at: 1613 1637 1868 4538 1613
Size: 24 231 2670 4760
Fragments arranged by size:
4760 2670 231 24
PmeI GTTT'AAAC
Cuts at: 4684 4684
Size: 7685
PmlI CAC'GTG
Cuts at: 1284 1646 1284
Size: 362 7323
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 811 843 1249 1281 6131 6163 811
Size: 32 406 32 4850 32 2333
Fragments arranged by size:
4850 2333 406 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3310 3310
Size: 7685
PshAI GACnn'nnGTC
Cuts at: 2600 3491 2600
Size: 891 6794
PsiI TTA'TAA
Cuts at: 275 275
Size: 7685
PspOMI G'GGCC_C
Cuts at: 1584 1946 2002 3425 1584
Size: 362 56 1423 5844
Fragments arranged by size:
5844 1423 362 56
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 221 253 221
Size: 32 7653
PstI C_TGCA'G
Cuts at: 583 662 1130 1907 2147 3374 3389 3407 3505
Size: 79 468 777 240 1227 15 18 98
Cuts at: 3505 4244 4520 4589 4732 5417 583
Size: 739 276 69 143 685 2851
Fragments arranged by size:
2851 1227 777 739 685 468 276 240
143 98 79 69 18 15
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7685
PvuII CAG'CTG
Cuts at: 780 1549 2136 2763 2823 3916 4501 5110 5470
Size: 769 587 627 60 1093 585 609 360
Cuts at: 5470 780
Size: 2995
Fragments arranged by size:
2995 1093 769 627 609 587 585 360
60
RsrII CG'GwC_CG
Cuts at: 5880 5880
Size: 7685
SacI G_AGCT'C
Cuts at: 1844 4619 4699 1844
Size: 2775 80 4830
Fragments arranged by size:
4830 2775 80
SalI G'TCGA_C
Cuts at: 498 4722 498
Size: 4224 3461
SapI GCTCTTCn'nnn_
Cuts at: 4234 5708 5918 4234
Size: 1474 210 6001
Fragments arranged by size:
6001 1474 210
SbfI CC_TGCA'GG
Cuts at: 583 1130 4732 583
Size: 547 3602 3536
Fragments arranged by size:
3602 3536 547
ScaI AGT'ACT
Cuts at: 7202 7202
Size: 7685
SexAI A'CCwGG_T
Cuts at: 3289 3289
Size: 7685
SfcI C'TryA_G
Cuts at: 33 579 658 1126 1903 1977 2143 2315 3370
Size: 546 79 468 777 74 166 172 1055
Cuts at: 3370 3385 3403 3501 4240 4516 4585 4728 5413
Size: 15 18 98 739 276 69 143 685
Cuts at: 5413 6381 6572 33
Size: 968 191 1146
Fragments arranged by size:
1146 1055 968 777 739 685 546 468
276 191 172 166 143 98 79 74
69 18 15
SfoI GGC'GCC
Cuts at: 3439 5117 5365 3439
Size: 1678 248 5759
Fragments arranged by size:
5759 1678 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7685
SmaI CCC'GGG
Cuts at: 3430 4707 3430
Size: 1277 6408
SmlI C'TyrA_G
Cuts at: 136 367 2923 3834 4633 4840 5755 6469 6731
Size: 231 2556 911 799 207 915 714 262
Cuts at: 6731 136
Size: 1090
Fragments arranged by size:
2556 1090 915 911 799 714 262 231
207
SpeI A'CTAG_T
Cuts at: 67 2173 67
Size: 2106 5579
SphI G_CATG'C
Cuts at: 1610 4738 5238 1610
Size: 3128 500 4057
Fragments arranged by size:
4057 3128 500
StyI C'CwwG_G
Cuts at: 107 636 938 2420 2876 3528 4819 5173 107
Size: 529 302 1482 456 652 1291 354 2619
Fragments arranged by size:
2619 1482 1291 652 529 456 354 302
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1332 3438 3817 5268 5401 5860 6069 1332
Size: 2106 379 1451 133 459 209 2948
Fragments arranged by size:
2948 2106 1451 459 379 209 133
TatI w'GTAC_w
Cuts at: 3027 4400 7200 3027
Size: 1373 2800 3512
Fragments arranged by size:
3512 2800 1373
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2283 2315 7202 7234 2283
Size: 32 4887 32 2734
Fragments arranged by size:
4887 2734 32 32
Tth111I GACn'n_nGTC
Cuts at: 5482 5482
Size: 7685
XbaI T'CTAG_A
Cuts at: 1041 4716 1041
Size: 3675 4010
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1176 5180 1176
Size: 4004 3681
XmaI C'CCGG_G
Cuts at: 3428 4705 3428
Size: 1277 6408
XmnI GAAnn'nnTTC
Cuts at: 4291 4291
Size: 7685
ZraI GAC'GTC
Cuts at: 4563 4563
Size: 7685
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AgeI AleI AscI AvrII BcgI BfrBI BmtI
BsiWI BsmBI BspEI BssHII BstZ17I CspCI FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PspXI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI