(Circular) (Six-base) MAPSORT of: pF1KA0136.seq Check: 5098 from: 1 to: 5930 pF1KA0136 5930 bp With 182 enzymes: SgfI * January 9, 2009 11:10 .. AccI GT'mk_AC Cuts at: 2968 2968 Size: 5930 Acc65I G'GTAC_C Cuts at: 2946 2946 Size: 5930 AclI AA'CG_TT Cuts at: 5770 5770 Size: 5930 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 345 1484 2054 2150 2696 2827 3755 4187 4540 Size: 1139 570 96 546 131 928 432 353 Cuts at: 4540 5202 345 Size: 662 1073 Fragments arranged by size: 1139 1073 928 662 570 546 432 353 131 96 AflII C'TTAA_G Cuts at: 1162 1162 Size: 5930 AflIII A'CryG_T Cuts at: 2481 3282 5082 5363 2481 Size: 801 1800 281 3048 Fragments arranged by size: 3048 1800 801 281 AlwNI CAG_nnn'CTG Cuts at: 208 3235 4673 208 Size: 3027 1438 1465 Fragments arranged by size: 3027 1465 1438 ApaLI G'TGCA_C Cuts at: 4768 4768 Size: 5930 ApoI r'AATT_y Cuts at: 171 767 985 1293 1525 1659 1800 2934 5283 Size: 596 218 308 232 134 141 1134 2349 Cuts at: 5283 5539 171 Size: 256 562 Fragments arranged by size: 2349 1134 596 562 308 256 232 218 141 134 AseI AT'TA_AT Cuts at: 20 214 578 1562 2384 20 Size: 194 364 984 822 3566 Fragments arranged by size: 3566 984 822 364 194 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5930 AvaI C'yCGr_G Cuts at: 772 2950 772 Size: 2178 3752 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2021 2054 2021 Size: 33 5897 BamHI G'GATC_C Cuts at: 2955 2955 Size: 5930 BanI G'GyrC_C Cuts at: 2946 3360 3608 3643 2946 Size: 414 248 35 5233 Fragments arranged by size: 5233 414 248 35 BanII G_rGCy'C Cuts at: 2944 3974 2944 Size: 1030 4900 BbeI G_GCGC'C Cuts at: 3364 3612 3364 Size: 248 5682 BbsI GAAGACnn'nnnn_ Cuts at: 934 1644 2081 934 Size: 710 437 4783 Fragments arranged by size: 4783 710 437 BbvCI CC'TCA_GC Cuts at: 139 139 Size: 5930 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 2152 2186 2152 Size: 34 5896 BciVI GTATCCnnnnn_n' Cuts at: 125 2291 3828 4884 5930 125 Size: 2166 1537 1056 1046 125 Fragments arranged by size: 2166 1537 1056 1046 125 BclI T'GATC_A Cuts at: 1326 2271 3450 1326 Size: 945 1179 3806 Fragments arranged by size: 3806 1179 945 BglI GCCn_nnn'nGGC Cuts at: 112 112 Size: 5930 BglII A'GATC_T Cuts at: 3445 5482 3445 Size: 2037 3893 BlpI GC'TnA_GC Cuts at: 349 3042 349 Size: 2693 3237 Bme1580I G_kGCm'C Cuts at: 3555 3648 4772 3555 Size: 93 1124 4713 Fragments arranged by size: 4713 1124 93 BmrI ACTGGGnnnn_n' Cuts at: 388 3142 3223 3558 5702 388 Size: 2754 81 335 2144 616 Fragments arranged by size: 2754 2144 616 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2791 4297 2791 Size: 1506 4424 Bpu10I CC'TnA_GC Cuts at: 139 139 Size: 5930 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3106 3985 4699 4997 3106 Size: 879 714 298 4039 Fragments arranged by size: 4039 879 714 298 BsaI GGTCTCn'nnnn_ Cuts at: 5634 5634 Size: 5930 BsaAI yAC'GTr Cuts at: 913 3283 913 Size: 2370 3560 BsaBI GATnn'nnATC Cuts at: 6 974 2837 3444 5481 6 Size: 968 1863 607 2037 455 Fragments arranged by size: 2037 1863 968 607 455 BsaHI Gr'CG_yC Cuts at: 3361 3609 4311 5807 3361 Size: 248 702 1496 3484 Fragments arranged by size: 3484 1496 702 248 BsaWI w'CCGG_w Cuts at: 1542 3119 3640 4318 4729 4876 5150 1542 Size: 1577 521 678 411 147 274 2322 Fragments arranged by size: 2322 1577 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1770 1800 1770 Size: 30 5900 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 2758 2758 Size: 5930 BseYI C'CCAG_C Cuts at: 3355 4113 4778 5186 3355 Size: 758 665 408 4099 Fragments arranged by size: 4099 758 665 408 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 221 2569 221 Size: 2348 3582 BsiEI CG_ry'CG Cuts at: 104 3472 3518 4748 104 Size: 3368 46 1230 1286 Fragments arranged by size: 3368 1286 1230 46 BsiHKAI G_wGCw'C Cuts at: 2944 3722 3912 4772 2944 Size: 778 190 860 4102 Fragments arranged by size: 4102 860 778 190 BsmI GAATG_Cn' Cuts at: 553 902 1770 2500 553 Size: 349 868 730 3983 Fragments arranged by size: 3983 868 730 349 Bsp1286I G_dGCh'C Cuts at: 2944 3555 3648 3722 3912 3974 4772 2944 Size: 611 93 74 190 62 798 4102 Fragments arranged by size: 4102 798 611 190 93 74 62 BspHI T'CATG_A Cuts at: 1881 4362 1881 Size: 2481 3449 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1878 2980 3194 3496 3877 1878 Size: 1102 214 302 381 3931 Fragments arranged by size: 3931 1102 381 302 214 BsrBI CCG'CTC Cuts at: 50 4222 4276 5760 5925 50 Size: 4172 54 1484 165 55 Fragments arranged by size: 4172 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 3842 3842 Size: 5930 BsrFI r'CCGG_y Cuts at: 3928 4109 3928 Size: 181 5749 BssHII G'CGCG_C Cuts at: 143 143 Size: 5930 BssSI C'ACGA_G Cuts at: 4201 4909 4201 Size: 708 5222 BstAPI GCAn_nnn'nTGC Cuts at: 2179 3307 2179 Size: 1128 4802 BstBI TT'CG_AA Cuts at: 943 4291 943 Size: 3348 2582 BstYI r'GATC_y Cuts at: 1 2955 3422 3445 3780 4026 4332 4344 4430 Size: 2954 467 23 335 246 306 12 86 Cuts at: 4430 5482 1 Size: 1052 449 Fragments arranged by size: 2954 1052 467 449 335 306 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 859 1619 859 Size: 760 5170 BtgI C'CryG_G Cuts at: 107 126 635 107 Size: 19 509 5402 Fragments arranged by size: 5402 509 19 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3880 4061 4224 3880 Size: 181 163 5586 Fragments arranged by size: 5586 181 163 BtsI GCAGTG_nn' Cuts at: 2108 3173 2108 Size: 1065 4865 DraI TTT'AAA Cuts at: 2929 2929 Size: 5930 DraIII CAC_nnn'GTG Cuts at: 2659 2659 Size: 5930 DrdI GACnn_nn'nnGTC Cuts at: 3636 4980 3636 Size: 1344 4586 EaeI y'GGCC_r Cuts at: 190 1582 1965 3515 3689 4080 4107 5452 190 Size: 1392 383 1550 174 391 27 1345 668 Fragments arranged by size: 1550 1392 1345 668 391 383 174 27 EagI C'GGCC_G Cuts at: 3515 3515 Size: 5930 EarI CTCTTCn'nnn_ Cuts at: 940 2795 3953 4163 940 Size: 1855 1158 210 2707 Fragments arranged by size: 2707 1855 1158 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1380 4138 4880 5026 5740 1380 Size: 2758 742 146 714 1570 Fragments arranged by size: 2758 1570 742 714 146 EcoICRI GAG'CTC Cuts at: 2942 2942 Size: 5930 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 345 1484 2054 2150 2696 2827 3755 4187 4540 Size: 1139 570 96 546 131 928 432 353 Cuts at: 4540 5202 345 Size: 662 1073 Fragments arranged by size: 1139 1073 928 662 570 546 432 353 131 96 EcoO109I rG'GnC_Cy Cuts at: 3069 3069 Size: 5930 EcoRI G'AATT_C Cuts at: 767 2934 767 Size: 2167 3763 EcoRV GAT'ATC Cuts at: 762 762 Size: 5930 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 619 1628 3949 619 Size: 1009 2321 2600 Fragments arranged by size: 2600 2321 1009 FspI TGC'GCA Cuts at: 3711 3711 Size: 5930 HaeII r_GCGC'y Cuts at: 3364 3612 4842 5613 3364 Size: 248 1230 771 3681 Fragments arranged by size: 3681 1230 771 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1882 1914 3772 3802 3804 3834 1882 Size: 32 1858 30 2 30 3978 Fragments arranged by size: 3978 1858 32 30 30 2 HincII GTy'rAC Cuts at: 2799 2969 2799 Size: 170 5760 HindIII A'AGCT_T Cuts at: 351 1082 1605 2432 351 Size: 731 523 827 3849 Fragments arranged by size: 3849 827 731 523 HpaI GTT'AAC Cuts at: 2799 2799 Size: 5930 KasI G'GCGC_C Cuts at: 3360 3608 3360 Size: 248 5682 KpnI G_GTAC'C Cuts at: 2950 2950 Size: 5930 MluI A'CGCG_T Cuts at: 5363 5363 Size: 5930 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 378 1077 1110 1119 4689 4873 378 Size: 699 33 9 3570 184 1435 Fragments arranged by size: 3570 1435 699 184 33 9 MscI TGG'CCA Cuts at: 192 1967 3691 192 Size: 1775 1724 2431 Fragments arranged by size: 2431 1775 1724 MslI CAynn'nnrTG Cuts at: 484 1998 2762 3919 4046 484 Size: 1514 764 1157 127 2368 Fragments arranged by size: 2368 1514 1157 764 127 MspA1I CmG'CkG Cuts at: 128 936 3041 3355 3715 4499 4744 128 Size: 808 2105 314 360 784 245 1314 Fragments arranged by size: 2105 1314 808 784 360 314 245 NaeI GCC'GGC Cuts at: 4111 4111 Size: 5930 NarI GG'CG_CC Cuts at: 3361 3609 3361 Size: 248 5682 NcoI C'CATG_G Cuts at: 107 107 Size: 5930 NdeI CA'TA_TG Cuts at: 2261 2411 2759 2261 Size: 150 348 5432 Fragments arranged by size: 5432 348 150 NgoMIV G'CCGG_C Cuts at: 4109 4109 Size: 5930 NspI r_CATG'y Cuts at: 535 2485 2983 3483 5086 535 Size: 1950 498 500 1603 1379 Fragments arranged by size: 1950 1603 1379 500 498 PciI A'CATG_T Cuts at: 2481 5082 2481 Size: 2601 3329 PmeI GTTT'AAAC Cuts at: 2929 2929 Size: 5930 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4376 4408 4376 Size: 32 5898 PsiI TTA'TAA Cuts at: 1811 2246 1811 Size: 435 5495 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 632 664 632 Size: 32 5898 PstI C_TGCA'G Cuts at: 1030 1875 2022 2175 2977 3662 1030 Size: 845 147 153 802 685 3298 Fragments arranged by size: 3298 845 802 685 153 147 PvuI CG_AT'CG Cuts at: 104 104 Size: 5930 PvuII CAG'CTG Cuts at: 936 3355 3715 936 Size: 2419 360 3151 Fragments arranged by size: 3151 2419 360 RsrII CG'GwC_CG Cuts at: 4125 4125 Size: 5930 SacI G_AGCT'C Cuts at: 2944 2944 Size: 5930 SacII CC_GC'GG Cuts at: 129 129 Size: 5930 SalI G'TCGA_C Cuts at: 2967 2967 Size: 5930 SapI GCTCTTCn'nnn_ Cuts at: 3953 4163 3953 Size: 210 5720 SbfI CC_TGCA'GG Cuts at: 2977 2977 Size: 5930 ScaI AGT'ACT Cuts at: 2922 5447 2922 Size: 2525 3405 SfcI C'TryA_G Cuts at: 33 1026 1871 2018 2171 2973 3658 4626 4817 Size: 993 845 147 153 802 685 968 191 Cuts at: 4817 33 Size: 1146 Fragments arranged by size: 1146 993 968 845 802 685 191 153 147 SfoI GGC'GCC Cuts at: 3362 3610 3362 Size: 248 5682 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5930 SmaI CCC'GGG Cuts at: 2952 2952 Size: 5930 SmlI C'TyrA_G Cuts at: 1162 3085 4000 4714 4976 1162 Size: 1923 915 714 262 2116 Fragments arranged by size: 2116 1923 915 714 262 SpeI A'CTAG_T Cuts at: 67 178 67 Size: 111 5819 SphI G_CATG'C Cuts at: 2983 3483 2983 Size: 500 5430 StyI C'CwwG_G Cuts at: 107 3064 3418 107 Size: 2957 354 2619 Fragments arranged by size: 2957 2619 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1114 1936 3513 3646 4105 4314 1114 Size: 822 1577 133 459 209 2730 Fragments arranged by size: 2730 1577 822 459 209 133 TatI w'GTAC_w Cuts at: 1205 2920 5445 1205 Size: 1715 2525 1690 Fragments arranged by size: 2525 1715 1690 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 908 940 5447 5479 908 Size: 32 4507 32 1359 Fragments arranged by size: 4507 1359 32 32 Tth111I GACn'n_nGTC Cuts at: 370 2255 3727 370 Size: 1885 1472 2573 Fragments arranged by size: 2573 1885 1472 XbaI T'CTAG_A Cuts at: 2961 2961 Size: 5930 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3425 3425 Size: 5930 XmaI C'CCGG_G Cuts at: 2950 2950 Size: 5930 XmnI GAAnn'nnTTC Cuts at: 415 1628 1659 415 Size: 1213 31 4686 Fragments arranged by size: 4686 1213 31 Enzymes that do cut: AccI Acc65I AclI AcuI AflII AflIII AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI PmeI PpiI PsiI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AgeI AhdI AleI AlfI AloI ApaI AscI AvrII BfrBI BmgBI BmtI BpmI BsiWI BsmBI BspEI BsrGI BstEII BstXI BstZ17I ClaI CspCI EcoNI FseI FspAI MfeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI