Restriction map for
pF1KA0138
(Circular) (Six-base) MAPSORT of: pF1KA0138.seq Check: 6089 from: 1 to: 5972
pF1KA0138 5972 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AccI GT'mk_AC
Cuts at: 1474 1631 3010 1474
Size: 157 1379 4436
Fragments arranged by size:
4436 1379 157
Acc65I G'GTAC_C
Cuts at: 2988 2988
Size: 5972
AclI AA'CG_TT
Cuts at: 5812 5812
Size: 5972
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 406 539 1739 3797 4229 4582 5244 253
Size: 153 133 1200 2058 432 353 662 981
Fragments arranged by size:
2058 1200 981 662 432 353 153 133
AflIII A'CryG_T
Cuts at: 3324 5124 5405 3324
Size: 1800 281 3891
Fragments arranged by size:
3891 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1941 1941
Size: 5972
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 842 1085 842
Size: 243 5729
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1988 2020 1988
Size: 32 5940
AlwNI CAG_nnn'CTG
Cuts at: 2136 3277 4715 2136
Size: 1141 1438 3393
Fragments arranged by size:
3393 1438 1141
ApaI G_GGCC'C
Cuts at: 145 2771 145
Size: 2626 3346
ApaLI G'TGCA_C
Cuts at: 4810 4810
Size: 5972
ApoI r'AATT_y
Cuts at: 2976 5325 5581 2976
Size: 2349 256 3367
Fragments arranged by size:
3367 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5972
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5972
AvaI C'yCGr_G
Cuts at: 124 138 165 1440 2059 2116 2287 2772 2992
Size: 14 27 1275 619 57 171 485 220
Cuts at: 2992 124
Size: 3104
Fragments arranged by size:
3104 1275 619 485 220 171 57 27
14
BamHI G'GATC_C
Cuts at: 2997 2997
Size: 5972
BanI G'GyrC_C
Cuts at: 2246 2698 2856 2988 3402 3650 3685 2246
Size: 452 158 132 414 248 35 4533
Fragments arranged by size:
4533 452 414 248 158 132 35
BanII G_rGCy'C
Cuts at: 145 995 1085 1109 1255 1449 1850 2771 2986
Size: 850 90 24 146 194 401 921 215
Cuts at: 2986 4016 145
Size: 1030 2101
Fragments arranged by size:
2101 1030 921 850 401 215 194 146
90 24
BbeI G_GCGC'C
Cuts at: 2250 3406 3654 2250
Size: 1156 248 4568
Fragments arranged by size:
4568 1156 248
BbsI GAAGACnn'nnnn_
Cuts at: 431 1166 1727 431
Size: 735 561 4676
Fragments arranged by size:
4676 735 561
BciVI GTATCCnnnnn_n'
Cuts at: 3870 4926 5972 3870
Size: 1056 1046 3870
Fragments arranged by size:
3870 1056 1046
BclI T'GATC_A
Cuts at: 1239 3492 1239
Size: 2253 3719
BglI GCCn_nnn'nGGC
Cuts at: 1041 2775 1041
Size: 1734 4238
BglII A'GATC_T
Cuts at: 3487 5524 3487
Size: 2037 3935
BlpI GC'TnA_GC
Cuts at: 196 632 3084 196
Size: 436 2452 3084
Fragments arranged by size:
3084 2452 436
Bme1580I G_kGCm'C
Cuts at: 145 2771 2786 3597 3690 4814 145
Size: 2626 15 811 93 1124 1303
Fragments arranged by size:
2626 1303 1124 811 93 15
BmrI ACTGGGnnnn_n'
Cuts at: 191 2655 3184 3265 3600 5744 191
Size: 2464 529 81 335 2144 419
Fragments arranged by size:
2464 2144 529 419 335 81
BmtI G_CTAG'C
Cuts at: 2723 2723
Size: 5972
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1908 4339 1908
Size: 2431 3541
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 487 524 835 2158 2188 487
Size: 37 311 1323 30 4271
Fragments arranged by size:
4271 1323 311 37 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 1360 3148 4027 4741 5039 271
Size: 1089 1788 879 714 298 1204
Fragments arranged by size:
1788 1204 1089 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 2414 2477 5676 2414
Size: 63 3199 2710
Fragments arranged by size:
3199 2710 63
BsaAI yAC'GTr
Cuts at: 603 2853 3325 603
Size: 2250 472 3250
Fragments arranged by size:
3250 2250 472
BsaBI GATnn'nnATC
Cuts at: 6 3486 5523 6
Size: 3480 2037 455
Fragments arranged by size:
3480 2037 455
BsaHI Gr'CG_yC
Cuts at: 2247 3403 3651 4353 5849 2247
Size: 1156 248 702 1496 2370
Fragments arranged by size:
2370 1496 1156 702 248
BsaWI w'CCGG_w
Cuts at: 711 900 3161 3682 4360 4771 4918 5192 711
Size: 189 2261 521 678 411 147 274 1491
Fragments arranged by size:
2261 1491 678 521 411 274 189 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1835 1865 1835
Size: 30 5942
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 409 1117 2251 2686 409
Size: 708 1134 435 3695
Fragments arranged by size:
3695 1134 708 435
BseYI C'CCAG_C
Cuts at: 1570 2570 3397 4155 4820 5228 1570
Size: 1000 827 758 665 408 2314
Fragments arranged by size:
2314 1000 827 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 593 2393 593
Size: 1800 4172
BsiEI CG_ry'CG
Cuts at: 104 1823 2553 3514 3560 4790 104
Size: 1719 730 961 46 1230 1286
Fragments arranged by size:
1719 1286 1230 961 730 46
BsiHKAI G_wGCw'C
Cuts at: 540 1085 1109 1255 2657 2678 2732 2986 3764
Size: 545 24 146 1402 21 54 254 778
Cuts at: 3764 3954 4814 540
Size: 190 860 1698
Fragments arranged by size:
1698 1402 860 778 545 254 190 146
54 24 21
BsmBI CGTCTCn'nnnn_
Cuts at: 431 431
Size: 5972
Bsp1286I G_dGCh'C
Cuts at: 145 540 995 1085 1109 1255 1449 1850 2657
Size: 395 455 90 24 146 194 401 807
Cuts at: 2657 2678 2732 2771 2786 2986 3597 3690 3764
Size: 21 54 39 15 200 611 93 74
Cuts at: 3764 3954 4016 4814 145
Size: 190 62 798 1303
Fragments arranged by size:
1303 807 798 611 455 401 395 200
194 190 146 93 90 74 62 54
39 24 21 15
BspEI T'CCGG_A
Cuts at: 711 900 711
Size: 189 5783
BspHI T'CATG_A
Cuts at: 4404 4404
Size: 5972
BspMI ACCTGCnnnn'nnnn_
Cuts at: 482 1865 3022 3236 3538 3919 482
Size: 1383 1157 214 302 381 2535
Fragments arranged by size:
2535 1383 1157 381 302 214
BsrBI CCG'CTC
Cuts at: 50 282 1815 2046 2076 2142 2157 2163 2280
Size: 232 1533 231 30 66 15 6 117
Cuts at: 2280 4264 4318 5802 5967 50
Size: 1984 54 1484 165 55
Fragments arranged by size:
1984 1533 1484 232 231 165 117 66
55 54 30 15 6
BsrDI GCAATG_nn'
Cuts at: 2360 3884 2360
Size: 1524 4448
BsrFI r'CCGG_y
Cuts at: 1050 3970 4151 1050
Size: 2920 181 2871
Fragments arranged by size:
2920 2871 181
BssSI C'ACGA_G
Cuts at: 4243 4951 4243
Size: 708 5264
BstAPI GCAn_nnn'nTGC
Cuts at: 3349 3349
Size: 5972
BstBI TT'CG_AA
Cuts at: 4333 4333
Size: 5972
BstXI CCAn_nnnn'nTGG
Cuts at: 2494 2494
Size: 5972
BstYI r'GATC_y
Cuts at: 1 315 1371 2003 2997 3464 3487 3822 4068
Size: 314 1056 632 994 467 23 335 246
Cuts at: 4068 4374 4386 4472 5524 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1056 1052 994 632 467 449 335 314
306 246 86 23 12
BtgI C'CryG_G
Cuts at: 107 995 1121 2520 2526 2547 2702 2796 107
Size: 888 126 1399 6 21 155 94 3283
Fragments arranged by size:
3283 1399 888 155 126 94 21 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1025 3922 4103 4266 1025
Size: 2897 181 163 2731
Fragments arranged by size:
2897 2731 181 163
BtsI GCAGTG_nn'
Cuts at: 540 3215 540
Size: 2675 3297
ClaI AT'CG_AT
Cuts at: 215 215
Size: 5972
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 827 862 827
Size: 35 5937
DraI TTT'AAA
Cuts at: 2971 2971
Size: 5972
DraIII CAC_nnn'GTG
Cuts at: 2833 2856 2833
Size: 23 5949
DrdI GACnn_nn'nnGTC
Cuts at: 3678 5022 3678
Size: 1344 4628
EaeI y'GGCC_r
Cuts at: 923 2332 2386 2523 2550 2637 2888 3557 3731
Size: 1409 54 137 27 87 251 669 174
Cuts at: 3731 4122 4149 5494 923
Size: 391 27 1345 1401
Fragments arranged by size:
1409 1401 1345 669 391 251 174 137
87 54 27 27
EagI C'GGCC_G
Cuts at: 2550 3557 2550
Size: 1007 4965
EarI CTCTTCn'nnn_
Cuts at: 299 869 899 1679 3995 4205 299
Size: 570 30 780 2316 210 2066
Fragments arranged by size:
2316 2066 780 570 210 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 241 2307 2882 2897 4180 4922 5068 5782
Size: 114 2066 575 15 1283 742 146 714
Cuts at: 5782 127
Size: 317
Fragments arranged by size:
2066 1283 742 714 575 317 146 114
15
EcoICRI GAG'CTC
Cuts at: 1083 1107 1253 2984 1083
Size: 24 146 1731 4071
Fragments arranged by size:
4071 1731 146 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 406 487 524 539 835 1739 2158 2188
Size: 153 81 37 15 296 904 419 30
Cuts at: 2188 3797 4229 4582 5244 253
Size: 1609 432 353 662 981
Fragments arranged by size:
1609 981 904 662 432 419 353 296
153 81 37 30 15
EcoO109I rG'GnC_Cy
Cuts at: 142 879 2297 2533 2757 2768 3111 142
Size: 737 1418 236 224 11 343 3003
Fragments arranged by size:
3003 1418 737 343 236 224 11
EcoRI G'AATT_C
Cuts at: 2976 2976
Size: 5972
EcoRV GAT'ATC
Cuts at: 2368 2368
Size: 5972
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 745 3991 745
Size: 3246 2726
FspI TGC'GCA
Cuts at: 3753 3753
Size: 5972
HaeII r_GCGC'y
Cuts at: 896 1238 1983 2130 2250 2547 3406 3654 4884
Size: 342 745 147 120 297 859 248 1230
Cuts at: 4884 5655 896
Size: 771 1213
Fragments arranged by size:
1230 1213 859 771 745 342 297 248
147 120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1523 1555 1835 1867 1890 1897 1922 1927 1929
Size: 32 280 32 23 7 25 5 2
Cuts at: 1929 1959 3814 3844 3846 3876 1523
Size: 30 1855 30 2 30 3619
Fragments arranged by size:
3619 1855 280 32 32 30 30 30
25 23 7 5 2 2
HincII GTy'rAC
Cuts at: 935 1475 1632 3011 935
Size: 540 157 1379 3896
Fragments arranged by size:
3896 1379 540 157
HindIII A'AGCT_T
Cuts at: 862 1579 862
Size: 717 5255
KasI G'GCGC_C
Cuts at: 2246 3402 3650 2246
Size: 1156 248 4568
Fragments arranged by size:
4568 1156 248
KpnI G_GTAC'C
Cuts at: 2992 2992
Size: 5972
MluI A'CGCG_T
Cuts at: 5405 5405
Size: 5972
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1306 1610 2612 4731 4915 1306
Size: 304 1002 2119 184 2363
Fragments arranged by size:
2363 2119 1002 304 184
MscI TGG'CCA
Cuts at: 925 2334 2525 2890 3733 925
Size: 1409 191 365 843 3164
Fragments arranged by size:
3164 1409 843 365 191
MslI CAynn'nnrTG
Cuts at: 502 1858 2185 2492 3961 4088 502
Size: 1356 327 307 1469 127 2386
Fragments arranged by size:
2386 1469 1356 327 307 127
MspA1I CmG'CkG
Cuts at: 615 997 1316 1343 1992 2040 2100 2133 2262
Size: 382 319 27 649 48 60 33 129
Cuts at: 2262 2664 2798 3083 3397 3757 4541 4786 615
Size: 402 134 285 314 360 784 245 1801
Fragments arranged by size:
1801 784 649 402 382 360 319 314
285 245 134 129 60 48 33 27
NaeI GCC'GGC
Cuts at: 1052 4153 1052
Size: 3101 2871
NarI GG'CG_CC
Cuts at: 2247 3403 3651 2247
Size: 1156 248 4568
Fragments arranged by size:
4568 1156 248
NcoI C'CATG_G
Cuts at: 107 2520 2702 107
Size: 2413 182 3377
Fragments arranged by size:
3377 2413 182
NgoMIV G'CCGG_C
Cuts at: 1050 4151 1050
Size: 3101 2871
NheI G'CTAG_C
Cuts at: 2719 2719
Size: 5972
NspI r_CATG'y
Cuts at: 649 2184 3025 3525 5128 649
Size: 1535 841 500 1603 1493
Fragments arranged by size:
1603 1535 1493 841 500
PasI CC'CwG_GG
Cuts at: 2630 2844 2630
Size: 214 5758
PciI A'CATG_T
Cuts at: 5124 5124
Size: 5972
PflMI CCAn_nnn'nTGG
Cuts at: 547 1012 2136 547
Size: 465 1124 4383
Fragments arranged by size:
4383 1124 465
PmeI GTTT'AAAC
Cuts at: 2971 2971
Size: 5972
PmlI CAC'GTG
Cuts at: 2853 2853
Size: 5972
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4418 4450 4418
Size: 32 5940
PpuMI rG'GwC_Cy
Cuts at: 879 2533 879
Size: 1654 4318
PspOMI G'GGCC_C
Cuts at: 141 2767 141
Size: 2626 3346
PspXI vC'TCGA_Gb
Cuts at: 2059 2116 2059
Size: 57 5915
PstI C_TGCA'G
Cuts at: 479 2114 3019 3704 479
Size: 1635 905 685 2747
Fragments arranged by size:
2747 1635 905 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5972
PvuII CAG'CTG
Cuts at: 615 2262 3397 3757 615
Size: 1647 1135 360 2830
Fragments arranged by size:
2830 1647 1135 360
RsrII CG'GwC_CG
Cuts at: 4167 4167
Size: 5972
SacI G_AGCT'C
Cuts at: 1085 1109 1255 2986 1085
Size: 24 146 1731 4071
Fragments arranged by size:
4071 1731 146 24
SacII CC_GC'GG
Cuts at: 998 2799 998
Size: 1801 4171
SalI G'TCGA_C
Cuts at: 1473 1630 3009 1473
Size: 157 1379 4436
Fragments arranged by size:
4436 1379 157
SapI GCTCTTCn'nnn_
Cuts at: 3995 4205 3995
Size: 210 5762
SbfI CC_TGCA'GG
Cuts at: 3019 3019
Size: 5972
ScaI AGT'ACT
Cuts at: 5489 5489
Size: 5972
SfcI C'TryA_G
Cuts at: 33 475 1037 2095 2110 3015 3700 4668 4859
Size: 442 562 1058 15 905 685 968 191
Cuts at: 4859 33
Size: 1146
Fragments arranged by size:
1146 1058 968 905 685 562 442 191
15
SfiI GGCCn_nnn'nGGCC
Cuts at: 2775 2775
Size: 5972
SfoI GGC'GCC
Cuts at: 2248 3404 3652 2248
Size: 1156 248 4568
Fragments arranged by size:
4568 1156 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5972
SmaI CCC'GGG
Cuts at: 126 167 1442 2289 2994 126
Size: 41 1275 847 705 3104
Fragments arranged by size:
3104 1275 847 705 41
SmlI C'TyrA_G
Cuts at: 286 1375 2059 2116 3127 4042 4756 5018 286
Size: 1089 684 57 1011 915 714 262 1240
Fragments arranged by size:
1240 1089 1011 915 714 684 262 57
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5972
SphI G_CATG'C
Cuts at: 649 2184 3025 3525 649
Size: 1535 841 500 3096
Fragments arranged by size:
3096 1535 841 500
SrfI GCCC'GGGC
Cuts at: 167 2289 167
Size: 2122 3850
StuI AGG'CCT
Cuts at: 1093 2065 1093
Size: 972 5000
StyI C'CwwG_G
Cuts at: 107 2520 2702 3106 3460 107
Size: 2413 182 404 354 2619
Fragments arranged by size:
2619 2413 404 354 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1134 1766 2327 2378 2936 3555 3688 4147 4356
Size: 632 561 51 558 619 133 459 209
Cuts at: 4356 1134
Size: 2750
Fragments arranged by size:
2750 632 619 561 558 459 209 133
51
TatI w'GTAC_w
Cuts at: 5487 5487
Size: 5972
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1014 1046 2333 2365 5489 5521 1014
Size: 32 1287 32 3124 32 1465
Fragments arranged by size:
3124 1465 1287 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3769 3769
Size: 5972
XbaI T'CTAG_A
Cuts at: 1780 3003 1780
Size: 1223 4749
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3467 3467
Size: 5972
XhoI C'TCGA_G
Cuts at: 2059 2116 2059
Size: 57 5915
XmaI C'CCGG_G
Cuts at: 124 165 1440 2287 2992 124
Size: 41 1275 847 705 3104
Fragments arranged by size:
3104 1275 847 705 41
XmnI GAAnn'nnTTC
Cuts at: 1384 1384
Size: 5972
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AscI AvrII
BaeI BbvCI BcgI BfrBI BmgBI Bpu10I BsiWI BsmI
BsrGI BssHII BstEII BstZ17I Bsu36I EcoNI FseI FspAI
HpaI MfeI NdeI NotI NruI NsiI PacI PfoI
PshAI PsiI PsrI SanDI SexAI SgrAI SnaBI SspI
SwaI ZraI