Restriction map for
pF1KA0143
(Circular) (Six-base) MAPSORT of: pF1KA0143.seq Check: 2116 from: 1 to: 5576
pF1KA0143 5576 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AccI GT'mk_AC
Cuts at: 2614 2614
Size: 5576
Acc65I G'GTAC_C
Cuts at: 2592 2592
Size: 5576
AclI AA'CG_TT
Cuts at: 1736 5416 1736
Size: 3680 1896
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 203 2013 2297 3401 3833 4580 4848 203
Size: 1810 284 1104 432 747 268 931
Fragments arranged by size:
1810 1104 931 747 432 284 268
AflIII A'CryG_T
Cuts at: 2928 4032 5009 2928
Size: 1104 977 3495
Fragments arranged by size:
3495 1104 977
AgeI A'CCGG_T
Cuts at: 3964 3964
Size: 5576
AhdI GACnn_n'nnGTC
Cuts at: 1540 1540
Size: 5576
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 948 948
Size: 5576
AlwNI CAG_nnn'CTG
Cuts at: 244 1059 2881 4448 244
Size: 815 1822 1567 1372
Fragments arranged by size:
1822 1567 1372 815
ApaLI G'TGCA_C
Cuts at: 4346 4346
Size: 5576
ApoI r'AATT_y
Cuts at: 468 481 919 1156 1400 1649 1985 2368 2421
Size: 13 438 237 244 249 336 383 53
Cuts at: 2421 2580 4929 5185 468
Size: 159 2349 256 859
Fragments arranged by size:
2349 859 438 383 336 256 249 244
237 159 53 13
AseI AT'TA_AT
Cuts at: 20 20
Size: 5576
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5576
AvaI C'yCGr_G
Cuts at: 116 1031 2596 116
Size: 915 1565 3096
Fragments arranged by size:
3096 1565 915
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1681 1714 1681
Size: 33 5543
BamHI G'GATC_C
Cuts at: 909 1491 2601 909
Size: 582 1110 3884
Fragments arranged by size:
3884 1110 582
BanI G'GyrC_C
Cuts at: 2592 3006 3254 3289 2592
Size: 414 248 35 4879
Fragments arranged by size:
4879 414 248 35
BanII G_rGCy'C
Cuts at: 2590 3620 2590
Size: 1030 4546
BbeI G_GCGC'C
Cuts at: 3010 3258 3010
Size: 248 5328
BbsI GAAGACnn'nnnn_
Cuts at: 506 1110 1462 1736 506
Size: 604 352 274 4346
Fragments arranged by size:
4346 604 352 274
BbvCI CC'TCA_GC
Cuts at: 680 680
Size: 5576
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 107 124 141 90
Size: 17 17 17 5525
Fragments arranged by size:
5525 17 17 17
BciVI GTATCCnnnnn_n'
Cuts at: 2226 3474 4235 5576 2226
Size: 1248 761 1341 2226
Fragments arranged by size:
2226 1341 1248 761
BclI T'GATC_A
Cuts at: 3096 3096
Size: 5576
BfrBI ATG'CAT
Cuts at: 1557 1557
Size: 5576
BglII A'GATC_T
Cuts at: 1420 2556 3091 5128 1420
Size: 1136 535 2037 1868
Fragments arranged by size:
2037 1868 1136 535
BlpI GC'TnA_GC
Cuts at: 2688 2688
Size: 5576
Bme1580I G_kGCm'C
Cuts at: 3201 3294 4350 3201
Size: 93 1056 4427
Fragments arranged by size:
4427 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2471 2527 2788 2869 3204 5348 2471
Size: 56 261 81 335 2144 2699
Fragments arranged by size:
2699 2144 335 261 81 56
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3943 3943
Size: 5576
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 233 233
Size: 5576
Bpu10I CC'TnA_GC
Cuts at: 680 680
Size: 5576
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2752 3631 4123 4421 2752
Size: 879 492 298 3907
Fragments arranged by size:
3907 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5280 5280
Size: 5576
BsaAI yAC'GTr
Cuts at: 2929 2929
Size: 5576
BsaBI GATnn'nnATC
Cuts at: 6 2177 3090 5127 6
Size: 2171 913 2037 455
Fragments arranged by size:
2171 2037 913 455
BsaHI Gr'CG_yC
Cuts at: 2352 3007 3255 3957 5453 2352
Size: 655 248 702 1496 2475
Fragments arranged by size:
2475 1496 702 655 248
BsaWI w'CCGG_w
Cuts at: 1614 2765 3286 3964 4238 4385 4796 1614
Size: 1151 521 678 274 147 411 2394
Fragments arranged by size:
2394 1151 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2462 2462
Size: 5576
BseYI C'CCAG_C
Cuts at: 831 3001 3759 4336 4832 831
Size: 2170 758 577 496 1575
Fragments arranged by size:
2170 1575 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1261 1454 1261
Size: 193 5383
BsiEI CG_ry'CG
Cuts at: 104 3118 3164 4372 104
Size: 3014 46 1208 1308
Fragments arranged by size:
3014 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2590 3368 3558 4350 2590
Size: 778 190 792 3816
Fragments arranged by size:
3816 792 778 190
BsmI GAATG_Cn'
Cuts at: 1251 1251
Size: 5576
BsmBI CGTCTCn'nnnn_
Cuts at: 1150 1150
Size: 5576
Bsp1286I G_dGCh'C
Cuts at: 2590 3201 3294 3368 3558 3620 4350 2590
Size: 611 93 74 190 62 730 3816
Fragments arranged by size:
3816 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4796 4796
Size: 5576
BspHI T'CATG_A
Cuts at: 1310 1575 1673 2430 4752 1310
Size: 265 98 757 2322 2134
Fragments arranged by size:
2322 2134 757 265 98
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1033 2626 2840 3142 3523 1033
Size: 1593 214 302 381 3086
Fragments arranged by size:
3086 1593 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3868 3922 5406 5571 50
Size: 3818 54 1484 165 55
Fragments arranged by size:
3818 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1076 1832 3488 1076
Size: 756 1656 3164
Fragments arranged by size:
3164 1656 756
BsrFI r'CCGG_y
Cuts at: 3574 3755 3964 3574
Size: 181 209 5186
Fragments arranged by size:
5186 209 181
BssSI C'ACGA_G
Cuts at: 3847 4205 3847
Size: 358 5218
BstAPI GCAn_nnn'nTGC
Cuts at: 244 1909 2953 244
Size: 1665 1044 2867
Fragments arranged by size:
2867 1665 1044
BstBI TT'CG_AA
Cuts at: 1160 1989 3937 1160
Size: 829 1948 2799
Fragments arranged by size:
2799 1948 829
BstEII G'GTnAC_C
Cuts at: 1434 1434
Size: 5576
BstXI CCAn_nnnn'nTGG
Cuts at: 1891 2226 2325 1891
Size: 335 99 5142
Fragments arranged by size:
5142 335 99
BstYI r'GATC_y
Cuts at: 1 186 909 1183 1420 1491 2556 2601 3068
Size: 185 723 274 237 71 1065 45 467
Cuts at: 3068 3091 3426 3672 4684 4770 4782 5128 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1065 1012 723 467 449 346 335 274
246 237 185 86 71 45 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3526 3707 3870 3526
Size: 181 163 5232
Fragments arranged by size:
5232 181 163
BtsI GCAGTG_nn'
Cuts at: 930 1200 1471 2819 930
Size: 270 271 1348 3687
Fragments arranged by size:
3687 1348 271 270
DraI TTT'AAA
Cuts at: 939 2575 939
Size: 1636 3940
DraIII CAC_nnn'GTG
Cuts at: 981 2407 981
Size: 1426 4150
DrdI GACnn_nn'nnGTC
Cuts at: 3282 4140 3282
Size: 858 4718
EaeI y'GGCC_r
Cuts at: 1829 3161 3335 3726 3753 5098 1829
Size: 1332 174 391 27 1345 2307
Fragments arranged by size:
2307 1345 1332 391 174 27
EagI C'GGCC_G
Cuts at: 3161 3161
Size: 5576
EarI CTCTTCn'nnn_
Cuts at: 782 3599 3809 782
Size: 2817 210 2549
Fragments arranged by size:
2817 2549 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3784 4094 4240 5386 3784
Size: 310 146 1146 3974
Fragments arranged by size:
3974 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2588 2588
Size: 5576
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 203 233 2013 2297 3401 3833 4580 4848 203
Size: 30 1780 284 1104 432 747 268 931
Fragments arranged by size:
1780 1104 931 747 432 284 268 30
EcoO109I rG'GnC_Cy
Cuts at: 754 2715 754
Size: 1961 3615
EcoRI G'AATT_C
Cuts at: 1156 1400 2580 1156
Size: 244 1180 4152
Fragments arranged by size:
4152 1180 244
EcoRV GAT'ATC
Cuts at: 1368 1368
Size: 5576
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 217 2200 3595 217
Size: 1983 1395 2198
Fragments arranged by size:
2198 1983 1395
FspI TGC'GCA
Cuts at: 884 3357 884
Size: 2473 3103
HaeII r_GCGC'y
Cuts at: 3010 3258 4280 5259 3010
Size: 248 1022 979 3327
Fragments arranged by size:
3327 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 493 525 1526 1558 2128 2160 2200 2221 2232
Size: 32 1001 32 570 32 40 21 11
Cuts at: 2232 2253 3418 3448 3450 3480 493
Size: 21 1165 30 2 30 2589
Fragments arranged by size:
2589 1165 1001 570 40 32 32 32
30 30 21 21 11 2
HincII GTy'rAC
Cuts at: 648 741 1200 1375 2615 648
Size: 93 459 175 1240 3609
Fragments arranged by size:
3609 1240 459 175 93
HindIII A'AGCT_T
Cuts at: 402 448 1591 402
Size: 46 1143 4387
Fragments arranged by size:
4387 1143 46
KasI G'GCGC_C
Cuts at: 3006 3254 3006
Size: 248 5328
KpnI G_GTAC'C
Cuts at: 2596 2596
Size: 5576
MfeI C'AATT_G
Cuts at: 2309 2309
Size: 5576
MluI A'CGCG_T
Cuts at: 5009 5009
Size: 5576
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1126 2264 4247 4431 1126
Size: 1138 1983 184 2271
Fragments arranged by size:
2271 1983 1138 184
MscI TGG'CCA
Cuts at: 1831 3337 1831
Size: 1506 4070
MslI CAynn'nnrTG
Cuts at: 1007 1889 2224 3565 3692 1007
Size: 882 335 1341 127 2891
Fragments arranged by size:
2891 1341 882 335 127
MspA1I CmG'CkG
Cuts at: 1695 2687 3001 3361 4374 4619 1695
Size: 992 314 360 1013 245 2652
Fragments arranged by size:
2652 1013 992 360 314 245
NaeI GCC'GGC
Cuts at: 3757 3757
Size: 5576
NarI GG'CG_CC
Cuts at: 3007 3255 3007
Size: 248 5328
NdeI CA'TA_TG
Cuts at: 413 686 413
Size: 273 5303
NgoMIV G'CCGG_C
Cuts at: 3755 3755
Size: 5576
NsiI A_TGCA'T
Cuts at: 1559 1559
Size: 5576
NspI r_CATG'y
Cuts at: 723 1969 2046 2515 2629 3129 4036 723
Size: 1246 77 469 114 500 907 2263
Fragments arranged by size:
2263 1246 907 500 469 114 77
PciI A'CATG_T
Cuts at: 4032 4032
Size: 5576
PflMI CCAn_nnn'nTGG
Cuts at: 196 671 1362 2451 196
Size: 475 691 1089 3321
Fragments arranged by size:
3321 1089 691 475
PfoI T'CCnGG_A
Cuts at: 986 1487 986
Size: 501 5075
PmeI GTTT'AAAC
Cuts at: 2575 2575
Size: 5576
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4715 4747 4715
Size: 32 5544
PsiI TTA'TAA
Cuts at: 946 1780 946
Size: 834 4742
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2447 2479 2447
Size: 32 5544
PstI C_TGCA'G
Cuts at: 2623 3308 2623
Size: 685 4891
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5576
PvuII CAG'CTG
Cuts at: 1695 3001 3361 1695
Size: 1306 360 3910
Fragments arranged by size:
3910 1306 360
RsrII CG'GwC_CG
Cuts at: 3771 3771
Size: 5576
SacI G_AGCT'C
Cuts at: 2590 2590
Size: 5576
SalI G'TCGA_C
Cuts at: 2613 2613
Size: 5576
SapI GCTCTTCn'nnn_
Cuts at: 3599 3809 3599
Size: 210 5366
SbfI CC_TGCA'GG
Cuts at: 2623 2623
Size: 5576
ScaI AGT'ACT
Cuts at: 5093 5093
Size: 5576
SfcI C'TryA_G
Cuts at: 33 1187 2619 3304 4297 4488 33
Size: 1154 1432 685 993 191 1121
Fragments arranged by size:
1432 1154 1121 993 685 191
SfoI GGC'GCC
Cuts at: 3008 3256 3008
Size: 248 5328
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5576
SmaI CCC'GGG
Cuts at: 1033 2598 1033
Size: 1565 4011
SmlI C'TyrA_G
Cuts at: 2731 3646 4138 4400 2731
Size: 915 492 262 3907
Fragments arranged by size:
3907 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5576
SphI G_CATG'C
Cuts at: 2046 2515 2629 3129 2046
Size: 469 114 500 4493
Fragments arranged by size:
4493 500 469 114
SspI AAT'ATT
Cuts at: 492 2450 492
Size: 1958 3618
StyI C'CwwG_G
Cuts at: 2710 3064 2710
Size: 354 5222
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3159 3292 3751 3960 3159
Size: 133 459 209 4775
Fragments arranged by size:
4775 459 209 133
TatI w'GTAC_w
Cuts at: 2081 5091 2081
Size: 3010 2566
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2451 2483 5093 5125 2451
Size: 32 2610 32 2902
Fragments arranged by size:
2902 2610 32 32
Tth111I GACn'n_nGTC
Cuts at: 3373 3373
Size: 5576
XbaI T'CTAG_A
Cuts at: 2188 2607 2188
Size: 419 5157
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3071 3071
Size: 5576
XmaI C'CCGG_G
Cuts at: 1031 2596 1031
Size: 1565 4011
XmnI GAAnn'nnTTC
Cuts at: 270 404 468 270
Size: 134 64 5378
Fragments arranged by size:
5378 134 64
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NsiI NspI PciI PflMI PfoI PmeI
PpiI PsiI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaI AscI
AvrII BglI BmgBI BmtI BsaXI BsiWI BsrGI BssHII
BstZ17I Bsu36I BtgI ClaI CspCI EcoNI FseI FspAI
HpaI NcoI NheI NotI NruI PacI PasI PmlI
PpuMI PshAI PspOMI PspXI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI ZraI