Restriction map for
pF1KA0144
(Circular) (Six-base) MAPSORT of: pF1KA0144.seq Check: 1713 from: 1 to: 6062
pF1KA0144 6062 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1434 1434
Size: 6062
AccI GT'mk_AC
Cuts at: 1756 1840 3100 1756
Size: 84 1260 4718
Fragments arranged by size:
4718 1260 84
Acc65I G'GTAC_C
Cuts at: 1910 3078 1910
Size: 1168 4894
AclI AA'CG_TT
Cuts at: 5902 5902
Size: 6062
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 866 887 1076 1396 1502 1953 2441 2569 3887
Size: 21 189 320 106 451 488 128 1318
Cuts at: 3887 4319 5066 5334 866
Size: 432 747 268 1594
Fragments arranged by size:
1594 1318 747 488 451 432 320 268
189 128 106 21
AflIII A'CryG_T
Cuts at: 3414 4518 5495 3414
Size: 1104 977 3981
Fragments arranged by size:
3981 1104 977
AgeI A'CCGG_T
Cuts at: 4450 4450
Size: 6062
AhdI GACnn_n'nnGTC
Cuts at: 1527 1527
Size: 6062
AleI CACnn'nnGTG
Cuts at: 2270 2272 2270
Size: 2 6060
AlwNI CAG_nnn'CTG
Cuts at: 1047 1560 1687 2029 2338 3367 4934 1047
Size: 513 127 342 309 1029 1567 2175
Fragments arranged by size:
2175 1567 1029 513 342 309 127
ApaI G_GGCC'C
Cuts at: 1876 1876
Size: 6062
ApaLI G'TGCA_C
Cuts at: 1348 4832 1348
Size: 3484 2578
ApoI r'AATT_y
Cuts at: 3066 5415 5671 3066
Size: 2349 256 3457
Fragments arranged by size:
3457 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6062
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6062
AvaI C'yCGr_G
Cuts at: 137 2859 3082 137
Size: 2722 223 3117
Fragments arranged by size:
3117 2722 223
AvrII C'CTAG_G
Cuts at: 2403 2403
Size: 6062
BamHI G'GATC_C
Cuts at: 1014 3087 1014
Size: 2073 3989
BanI G'GyrC_C
Cuts at: 535 583 1910 3078 3492 3740 3775 535
Size: 48 1327 1168 414 248 35 2822
Fragments arranged by size:
2822 1327 1168 414 248 48 35
BanII G_rGCy'C
Cuts at: 138 1288 1876 3076 4106 138
Size: 1150 588 1200 1030 2094
Fragments arranged by size:
2094 1200 1150 1030 588
BbeI G_GCGC'C
Cuts at: 3496 3744 3496
Size: 248 5814
BbsI GAAGACnn'nnnn_
Cuts at: 893 982 1457 893
Size: 89 475 5498
Fragments arranged by size:
5498 475 89
BbvCI CC'TCA_GC
Cuts at: 1541 2398 1541
Size: 857 5205
BciVI GTATCCnnnnn_n'
Cuts at: 2992 3960 4721 6062 2992
Size: 968 761 1341 2992
Fragments arranged by size:
2992 1341 968 761
BclI T'GATC_A
Cuts at: 3582 3582
Size: 6062
BfrBI ATG'CAT
Cuts at: 2953 2953
Size: 6062
BglII A'GATC_T
Cuts at: 861 878 3577 5614 861
Size: 17 2699 2037 1309
Fragments arranged by size:
2699 2037 1309 17
BlpI GC'TnA_GC
Cuts at: 1238 1550 1629 3174 1238
Size: 312 79 1545 4126
Fragments arranged by size:
4126 1545 312 79
Bme1580I G_kGCm'C
Cuts at: 127 1352 1876 3687 3780 4836 127
Size: 1225 524 1811 93 1056 1353
Fragments arranged by size:
1811 1353 1225 1056 524 93
BmgBI CAC'GTC
Cuts at: 508 508
Size: 6062
BmrI ACTGGGnnnn_n'
Cuts at: 156 1182 2344 2657 3274 3355 3690 5834 156
Size: 1026 1162 313 617 81 335 2144 384
Fragments arranged by size:
2144 1162 1026 617 384 335 313 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4429 4429
Size: 6062
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2289 2352 2510 2289
Size: 63 158 5841
Fragments arranged by size:
5841 158 63
Bpu10I CC'TnA_GC
Cuts at: 1541 1868 2398 1541
Size: 327 530 5205
Fragments arranged by size:
5205 530 327
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 671 2163 3238 4117 4609 4907 671
Size: 1492 1075 879 492 298 1826
Fragments arranged by size:
1826 1492 1075 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 473 865 2996 5766 473
Size: 392 2131 2770 769
Fragments arranged by size:
2770 2131 769 392
BsaAI yAC'GTr
Cuts at: 770 3415 770
Size: 2645 3417
BsaBI GATnn'nnATC
Cuts at: 6 3576 5613 6
Size: 3570 2037 455
Fragments arranged by size:
3570 2037 455
BsaHI Gr'CG_yC
Cuts at: 3493 3741 4443 5939 3493
Size: 248 702 1496 3616
Fragments arranged by size:
3616 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3251 3772 4450 4724 4871 5282 3251
Size: 521 678 274 147 411 4031
Fragments arranged by size:
4031 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1479 1509 1903 1933 2643 2673 1479
Size: 30 394 30 710 30 4868
Fragments arranged by size:
4868 710 394 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 274 683 847 1260 1460 2170 2355 2459 2709
Size: 409 164 413 200 710 185 104 250
Cuts at: 2709 274
Size: 3627
Fragments arranged by size:
3627 710 413 409 250 200 185 164
104
BseYI C'CCAG_C
Cuts at: 708 969 1040 1915 2724 2869 3487 4245 4822
Size: 261 71 875 809 145 618 758 577
Cuts at: 4822 5318 708
Size: 496 1452
Fragments arranged by size:
1452 875 809 758 618 577 496 261
145 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1333 1433 1999 2324 1333
Size: 100 566 325 5071
Fragments arranged by size:
5071 566 325 100
BsiEI CG_ry'CG
Cuts at: 104 2706 3604 3650 4858 104
Size: 2602 898 46 1208 1308
Fragments arranged by size:
2602 1308 1208 898 46
BsiHKAI G_wGCw'C
Cuts at: 1352 3076 3854 4044 4836 1352
Size: 1724 778 190 792 2578
Fragments arranged by size:
2578 1724 792 778 190
BsmI GAATG_Cn'
Cuts at: 387 1242 2121 2955 387
Size: 855 879 834 3494
Fragments arranged by size:
3494 879 855 834
BsmBI CGTCTCn'nnnn_
Cuts at: 1025 1025
Size: 6062
Bsp1286I G_dGCh'C
Cuts at: 127 138 1288 1352 1876 3076 3687 3780 3854
Size: 11 1150 64 524 1200 611 93 74
Cuts at: 3854 4044 4106 4836 127
Size: 190 62 730 1353
Fragments arranged by size:
1353 1200 1150 730 611 524 190 93
74 64 62 11
BspEI T'CCGG_A
Cuts at: 5282 5282
Size: 6062
BspHI T'CATG_A
Cuts at: 5238 5238
Size: 6062
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1434 1661 3112 3326 3628 4009 1434
Size: 227 1451 214 302 381 3487
Fragments arranged by size:
3487 1451 381 302 227 214
BsrBI CCG'CTC
Cuts at: 50 1826 4354 4408 5892 6057 50
Size: 1776 2528 54 1484 165 55
Fragments arranged by size:
2528 1776 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 339 1666 3974 339
Size: 1327 2308 2427
Fragments arranged by size:
2427 2308 1327
BsrFI r'CCGG_y
Cuts at: 1545 4060 4241 4450 1545
Size: 2515 181 209 3157
Fragments arranged by size:
3157 2515 209 181
BssSI C'ACGA_G
Cuts at: 1719 4333 4691 1719
Size: 2614 358 3090
Fragments arranged by size:
3090 2614 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3439 3439
Size: 6062
BstBI TT'CG_AA
Cuts at: 1063 4423 1063
Size: 3360 2702
BstEII G'GTnAC_C
Cuts at: 2686 2686
Size: 6062
BstXI CCAn_nnnn'nTGG
Cuts at: 436 2742 436
Size: 2306 3756
BstYI r'GATC_y
Cuts at: 1 861 878 1014 1513 1891 3087 3554 3577
Size: 860 17 136 499 378 1196 467 23
Cuts at: 3577 3912 4158 5170 5256 5268 5614 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1196 1012 860 499 467 449 378 346
335 246 136 86 23 17 12
BstZ17I GTA'TAC
Cuts at: 1757 1757
Size: 6062
BtgI C'CryG_G
Cuts at: 543 636 2706 543
Size: 93 2070 3899
Fragments arranged by size:
3899 2070 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4012 4193 4356 4012
Size: 181 163 5718
Fragments arranged by size:
5718 181 163
BtsI GCAGTG_nn'
Cuts at: 200 884 935 1352 1436 2039 3305 200
Size: 684 51 417 84 603 1266 2957
Fragments arranged by size:
2957 1266 684 603 417 84 51
DraI TTT'AAA
Cuts at: 3061 3061
Size: 6062
DrdI GACnn_nn'nnGTC
Cuts at: 3768 4626 3768
Size: 858 5204
EaeI y'GGCC_r
Cuts at: 190 441 639 783 1766 2666 2703 2732 2957
Size: 251 198 144 983 900 37 29 225
Cuts at: 2957 3647 3821 4212 4239 5584 190
Size: 690 174 391 27 1345 668
Fragments arranged by size:
1345 983 900 690 668 391 251 225
198 174 144 37 29 27
EagI C'GGCC_G
Cuts at: 2703 3647 2703
Size: 944 5118
EarI CTCTTCn'nnn_
Cuts at: 603 1734 1932 2922 4085 4295 603
Size: 1131 198 990 1163 210 2370
Fragments arranged by size:
2370 1163 1131 990 210 198
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4270 4580 4726 5872 4270
Size: 310 146 1146 4460
Fragments arranged by size:
4460 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3074 3074
Size: 6062
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 866 887 1076 1396 1502 1953 2289 2352 2441
Size: 21 189 320 106 451 336 63 89
Cuts at: 2441 2510 2569 3887 4319 5066 5334 866
Size: 69 59 1318 432 747 268 1594
Fragments arranged by size:
1594 1318 747 451 432 336 320 268
189 106 89 69 63 59 21
EcoNI CCTnn'n_nnAGG
Cuts at: 1091 1951 1091
Size: 860 5202
EcoO109I rG'GnC_Cy
Cuts at: 598 1872 1873 1944 2490 2856 3201 598
Size: 1274 1 71 546 366 345 3459
Fragments arranged by size:
3459 1274 546 366 345 71 1
EcoRI G'AATT_C
Cuts at: 3066 3066
Size: 6062
EcoRV GAT'ATC
Cuts at: 1638 1638
Size: 6062
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1983 4081 1983
Size: 2098 3964
FspI TGC'GCA
Cuts at: 3843 3843
Size: 6062
HaeII r_GCGC'y
Cuts at: 1371 1727 2812 3496 3744 4766 5745 1371
Size: 356 1085 684 248 1022 979 1688
Fragments arranged by size:
1688 1085 1022 979 684 356 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1270 1302 1513 1545 1590 1622 1690 1722 2100
Size: 32 211 32 45 32 68 32 378
Cuts at: 2100 2132 2241 2273 2704 2736 2757 2789 3904
Size: 32 109 32 431 32 21 32 1115
Cuts at: 3904 3934 3936 3966 1270
Size: 30 2 30 3366
Fragments arranged by size:
3366 1115 431 378 211 109 68 45
32 32 32 32 32 32 32 32
30 30 21 2
HincII GTy'rAC
Cuts at: 345 1841 3101 345
Size: 1496 1260 3306
Fragments arranged by size:
3306 1496 1260
KasI G'GCGC_C
Cuts at: 3492 3740 3492
Size: 248 5814
KpnI G_GTAC'C
Cuts at: 1914 3082 1914
Size: 1168 4894
MfeI C'AATT_G
Cuts at: 274 274
Size: 6062
MluI A'CGCG_T
Cuts at: 5495 5495
Size: 6062
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2994 4733 4917 2994
Size: 1739 184 4139
Fragments arranged by size:
4139 1739 184
MscI TGG'CCA
Cuts at: 443 785 2668 3823 443
Size: 342 1883 1155 2682
Fragments arranged by size:
2682 1883 1155 342
MslI CAynn'nnrTG
Cuts at: 245 2270 2272 4051 4178 245
Size: 2025 2 1779 127 2129
Fragments arranged by size:
2129 2025 1779 127 2
MspA1I CmG'CkG
Cuts at: 189 712 761 1447 2643 3173 3487 3847 4860
Size: 523 49 686 1196 530 314 360 1013
Cuts at: 4860 5105 189
Size: 245 1146
Fragments arranged by size:
1196 1146 1013 686 530 523 360 314
245 49
NaeI GCC'GGC
Cuts at: 1547 4243 1547
Size: 2696 3366
NarI GG'CG_CC
Cuts at: 3493 3741 3493
Size: 248 5814
NgoMIV G'CCGG_C
Cuts at: 1545 4241 1545
Size: 2696 3366
NsiI A_TGCA'T
Cuts at: 2955 2955
Size: 6062
NspI r_CATG'y
Cuts at: 1131 2542 3115 3615 4522 1131
Size: 1411 573 500 907 2671
Fragments arranged by size:
2671 1411 907 573 500
PciI A'CATG_T
Cuts at: 4518 4518
Size: 6062
PflMI CCAn_nnn'nTGG
Cuts at: 802 1047 1194 1221 802
Size: 245 147 27 5643
Fragments arranged by size:
5643 245 147 27
PmeI GTTT'AAAC
Cuts at: 3061 3061
Size: 6062
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5201 5233 5201
Size: 32 6030
PpuMI rG'GwC_Cy
Cuts at: 1944 2490 2856 1944
Size: 546 366 5150
Fragments arranged by size:
5150 546 366
PshAI GACnn'nnGTC
Cuts at: 497 497
Size: 6062
PspOMI G'GGCC_C
Cuts at: 1872 1872
Size: 6062
PstI C_TGCA'G
Cuts at: 207 1431 1658 2034 2094 3109 3794 207
Size: 1224 227 376 60 1015 685 2475
Fragments arranged by size:
2475 1224 1015 685 376 227 60
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6062
PvuII CAG'CTG
Cuts at: 712 1447 3487 3847 712
Size: 735 2040 360 2927
Fragments arranged by size:
2927 2040 735 360
RsrII CG'GwC_CG
Cuts at: 4257 4257
Size: 6062
SacI G_AGCT'C
Cuts at: 3076 3076
Size: 6062
SalI G'TCGA_C
Cuts at: 1839 3099 1839
Size: 1260 4802
SanDI GG'GwC_CC
Cuts at: 2490 2856 2490
Size: 366 5696
SapI GCTCTTCn'nnn_
Cuts at: 4085 4295 4085
Size: 210 5852
SbfI CC_TGCA'GG
Cuts at: 3109 3109
Size: 6062
ScaI AGT'ACT
Cuts at: 1176 5579 1176
Size: 4403 1659
SfcI C'TryA_G
Cuts at: 33 203 495 1178 1427 1654 2021 2030 2090
Size: 170 292 683 249 227 367 9 60
Cuts at: 2090 2610 2823 3105 3790 4783 4974 33
Size: 520 213 282 685 993 191 1121
Fragments arranged by size:
1121 993 685 683 520 367 292 282
249 227 213 191 170 60 9
SfoI GGC'GCC
Cuts at: 3494 3742 3494
Size: 248 5814
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6062
SmaI CCC'GGG
Cuts at: 139 2861 3084 139
Size: 2722 223 3117
Fragments arranged by size:
3117 2722 223
SmlI C'TyrA_G
Cuts at: 686 2142 3217 4132 4624 4886 686
Size: 1456 1075 915 492 262 1862
Fragments arranged by size:
1862 1456 1075 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6062
SphI G_CATG'C
Cuts at: 3115 3615 3115
Size: 500 5562
StyI C'CwwG_G
Cuts at: 2132 2403 2741 3196 3550 2132
Size: 271 338 455 354 4644
Fragments arranged by size:
4644 455 354 338 271
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2125 2646 3645 3778 4237 4446 2125
Size: 521 999 133 459 209 3741
Fragments arranged by size:
3741 999 521 459 209 133
TatI w'GTAC_w
Cuts at: 1174 5577 1174
Size: 4403 1659
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1479 1511 5579 5611 1479
Size: 32 4068 32 1930
Fragments arranged by size:
4068 1930 32 32
Tth111I GACn'n_nGTC
Cuts at: 3859 3859
Size: 6062
XbaI T'CTAG_A
Cuts at: 3093 3093
Size: 6062
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 436 2977 3557 436
Size: 2541 580 2941
Fragments arranged by size:
2941 2541 580
XmaI C'CCGG_G
Cuts at: 137 2859 3082 137
Size: 2722 223 3117
Fragments arranged by size:
3117 2722 223
XmnI GAAnn'nnTTC
Cuts at: 678 1966 678
Size: 1288 4774
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NsiI NspI PciI
PflMI PmeI PpiI PpuMI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI AscI BaeI BcgI
BglI BmtI BsiWI BsrGI BssHII Bsu36I ClaI CspCI
DraIII FseI FspAI HindIII HpaI NcoI NdeI NheI
NotI NruI PacI PasI PfoI PmlI PsiI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI