Restriction map for
pF1KA0159
(Circular) (Six-base) MAPSORT of: pF1KA0159.seq Check: 6779 from: 1 to: 7316
pF1KA0159 7316 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 791 2538 791
Size: 1747 5569
AatII G_ACGT'C
Cuts at: 1344 1344
Size: 7316
AccI GT'mk_AC
Cuts at: 1570 1861 4354 1570
Size: 291 2493 4532
Fragments arranged by size:
4532 2493 291
Acc65I G'GTAC_C
Cuts at: 2109 4079 4332 2109
Size: 1970 253 5093
Fragments arranged by size:
5093 1970 253
AclI AA'CG_TT
Cuts at: 7156 7156
Size: 7316
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 601 622 974 1667 1737 3870 5141 5573 6320
Size: 21 352 693 70 2133 1271 432 747
Cuts at: 6320 6588 601
Size: 268 1329
Fragments arranged by size:
2133 1329 1271 747 693 432 352 268
70 21
AfeI AGC'GCT
Cuts at: 2419 2419
Size: 7316
AflIII A'CryG_T
Cuts at: 1035 1306 4668 5772 6749 1035
Size: 271 3362 1104 977 1602
Fragments arranged by size:
3362 1602 1104 977 271
AgeI A'CCGG_T
Cuts at: 5704 5704
Size: 7316
AhdI GACnn_n'nnGTC
Cuts at: 1888 3867 1888
Size: 1979 5337
AleI CACnn'nnGTG
Cuts at: 2494 2494
Size: 7316
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2801 2801
Size: 7316
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2872 2904 2872
Size: 32 7284
AlwNI CAG_nnn'CTG
Cuts at: 849 1389 1516 1977 2121 2373 2799 2919 3138
Size: 540 127 461 144 252 426 120 219
Cuts at: 3138 4089 4621 6188 849
Size: 951 532 1567 1977
Fragments arranged by size:
1977 1567 951 540 532 461 426 252
219 144 127 120
ApaI G_GGCC'C
Cuts at: 266 1494 2752 266
Size: 1228 1258 4830
Fragments arranged by size:
4830 1258 1228
ApaLI G'TGCA_C
Cuts at: 6086 6086
Size: 7316
ApoI r'AATT_y
Cuts at: 355 685 2080 2318 3958 4320 6669 6925 355
Size: 330 1395 238 1640 362 2349 256 746
Fragments arranged by size:
2349 1640 1395 746 362 330 256 238
AseI AT'TA_AT
Cuts at: 20 740 20
Size: 720 6596
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7316
AvaI C'yCGr_G
Cuts at: 764 1181 1715 1853 3529 3637 4336 764
Size: 417 534 138 1676 108 699 3744
Fragments arranged by size:
3744 1676 699 534 417 138 108
BamHI G'GATC_C
Cuts at: 1917 2699 4341 1917
Size: 782 1642 4892
Fragments arranged by size:
4892 1642 782
BanI G'GyrC_C
Cuts at: 870 2109 2960 4079 4332 4746 4994 5029 870
Size: 1239 851 1119 253 414 248 35 3157
Fragments arranged by size:
3157 1239 1119 851 414 253 248 35
BanII G_rGCy'C
Cuts at: 258 266 1494 2752 2954 3025 3575 4133 4330
Size: 8 1228 1258 202 71 550 558 197
Cuts at: 4330 5360 258
Size: 1030 2214
Fragments arranged by size:
2214 1258 1228 1030 558 550 202 197
71 8
BbeI G_GCGC'C
Cuts at: 2964 4750 4998 2964
Size: 1786 248 5282
Fragments arranged by size:
5282 1786 248
BbsI GAAGACnn'nnnn_
Cuts at: 2088 3271 4191 4256 2088
Size: 1183 920 65 5148
Fragments arranged by size:
5148 1183 920 65
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 298 332 3955 3989 298
Size: 34 3623 34 3625
Fragments arranged by size:
3625 3623 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 384 1200 5214 5975 7316 384
Size: 816 4014 761 1341 384
Fragments arranged by size:
4014 1341 816 761 384
BclI T'GATC_A
Cuts at: 3245 4836 3245
Size: 1591 5725
BglII A'GATC_T
Cuts at: 4227 4831 6868 4227
Size: 604 2037 4675
Fragments arranged by size:
4675 2037 604
BlpI GC'TnA_GC
Cuts at: 3422 4428 3422
Size: 1006 6310
Bme1580I G_kGCm'C
Cuts at: 266 1494 2752 2769 4941 5034 6090 266
Size: 1228 1258 17 2172 93 1056 1492
Fragments arranged by size:
2172 1492 1258 1228 1056 93 17
BmgBI CAC'GTC
Cuts at: 3922 3922
Size: 7316
BmrI ACTGGGnnnn_n'
Cuts at: 600 3353 4528 4609 4944 7088 600
Size: 2753 1175 81 335 2144 828
Fragments arranged by size:
2753 2144 1175 828 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5683 5683
Size: 7316
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 745 2434 2821 745
Size: 1689 387 5240
Fragments arranged by size:
5240 1689 387
Bpu10I CC'TnA_GC
Cuts at: 257 1619 257
Size: 1362 5954
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2332 3454 4036 4188 4492 5371 5863 6161 2332
Size: 1122 582 152 304 879 492 298 3487
Fragments arranged by size:
3487 1122 879 582 492 304 298 152
BsaI GGTCTCn'nnnn_
Cuts at: 496 977 1187 1459 1777 3022 3773 7020 496
Size: 481 210 272 318 1245 751 3247 792
Fragments arranged by size:
3247 1245 792 751 481 318 272 210
BsaAI yAC'GTr
Cuts at: 4669 4669
Size: 7316
BsaBI GATnn'nnATC
Cuts at: 6 4830 6867 6
Size: 4824 2037 455
Fragments arranged by size:
4824 2037 455
BsaHI Gr'CG_yC
Cuts at: 1341 2961 4747 4995 5697 7193 1341
Size: 1620 1786 248 702 1496 1464
Fragments arranged by size:
1786 1620 1496 1464 702 248
BsaWI w'CCGG_w
Cuts at: 3320 4505 5026 5704 5978 6125 6536 3320
Size: 1185 521 678 274 147 411 4100
Fragments arranged by size:
4100 1185 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 926 956 1981 2011 926
Size: 30 1025 30 6231
Fragments arranged by size:
6231 1025 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 166 1549 1552 1621 1624 1786 1795 3055 3058
Size: 1383 3 69 3 162 9 1260 3
Cuts at: 3058 3380 3676 166
Size: 322 296 3806
Fragments arranged by size:
3806 1383 1260 322 296 162 69 9
3 3 3
BseYI C'CCAG_C
Cuts at: 1041 1824 2366 2543 2896 3048 3269 3642 4163
Size: 783 542 177 353 152 221 373 521
Cuts at: 4163 4741 5499 6076 6572 1041
Size: 578 758 577 496 1785
Fragments arranged by size:
1785 783 758 578 577 542 521 496
373 353 221 177 152
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2086 2356 2086
Size: 270 7046
BsiEI CG_ry'CG
Cuts at: 104 4858 4904 6112 104
Size: 4754 46 1208 1308
Fragments arranged by size:
4754 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 258 2954 2990 3025 3575 4330 5108 5298 6090
Size: 2696 36 35 550 755 778 190 792
Cuts at: 6090 258
Size: 1484
Fragments arranged by size:
2696 1484 792 778 755 550 190 36
35
BsmI GAATG_Cn'
Cuts at: 1012 1012
Size: 7316
BsmBI CGTCTCn'nnnn_
Cuts at: 490 490
Size: 7316
Bsp1286I G_dGCh'C
Cuts at: 258 266 1494 2752 2769 2954 2990 3025 3575
Size: 8 1228 1258 17 185 36 35 550
Cuts at: 3575 4133 4330 4941 5034 5108 5298 5360 6090
Size: 558 197 611 93 74 190 62 730
Cuts at: 6090 258
Size: 1484
Fragments arranged by size:
1484 1258 1228 730 611 558 550 197
190 185 93 74 62 36 35 17
8
BspEI T'CCGG_A
Cuts at: 6536 6536
Size: 7316
BspHI T'CATG_A
Cuts at: 3674 6492 3674
Size: 2818 4498
BspMI ACCTGCnnnn'nnnn_
Cuts at: 791 1137 2538 2891 3486 4021 4366 4580 4882
Size: 346 1401 353 595 535 345 214 302
Cuts at: 4882 5263 791
Size: 381 2844
Fragments arranged by size:
2844 1401 595 535 381 353 346 345
302 214
BsrBI CCG'CTC
Cuts at: 50 2382 3768 5608 5662 7146 7311 50
Size: 2332 1386 1840 54 1484 165 55
Fragments arranged by size:
2332 1840 1484 1386 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2561 2861 5228 2561
Size: 300 2367 4649
Fragments arranged by size:
4649 2367 300
BsrFI r'CCGG_y
Cuts at: 2957 5314 5495 5704 2957
Size: 2357 181 209 4569
Fragments arranged by size:
4569 2357 209 181
BssSI C'ACGA_G
Cuts at: 2453 5587 5945 2453
Size: 3134 358 3824
Fragments arranged by size:
3824 3134 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1516 1962 3239 4693 1516
Size: 446 1277 1454 4139
Fragments arranged by size:
4139 1454 1277 446
BstBI TT'CG_AA
Cuts at: 318 5677 318
Size: 5359 1957
BstXI CCAn_nnnn'nTGG
Cuts at: 3375 3996 3375
Size: 621 6695
BstYI r'GATC_y
Cuts at: 1 327 1917 2699 3000 3348 4227 4306 4341
Size: 326 1590 782 301 348 879 79 35
Cuts at: 4341 4808 4831 5166 5412 6424 6510 6522 6868
Size: 467 23 335 246 1012 86 12 346
Cuts at: 6868 1
Size: 449
Fragments arranged by size:
1590 1012 879 782 467 449 348 346
335 326 301 246 86 79 35 23
12
Bsu36I CC'TnA_GG
Cuts at: 1922 2627 3935 1922
Size: 705 1308 5303
Fragments arranged by size:
5303 1308 705
BtgI C'CryG_G
Cuts at: 107 506 579 1127 1221 3035 3096 107
Size: 399 73 548 94 1814 61 4327
Fragments arranged by size:
4327 1814 548 399 94 73 61
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5266 5447 5610 5266
Size: 181 163 6972
Fragments arranged by size:
6972 181 163
BtsI GCAGTG_nn'
Cuts at: 1325 1453 1526 2366 2726 2945 4559 1325
Size: 128 73 840 360 219 1614 4082
Fragments arranged by size:
4082 1614 840 360 219 128 73
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 509 544 2042 2077 509
Size: 35 1498 35 5748
Fragments arranged by size:
5748 1498 35 35
DraI TTT'AAA
Cuts at: 4315 4315
Size: 7316
DrdI GACnn_nn'nnGTC
Cuts at: 962 5022 5880 962
Size: 4060 858 2398
Fragments arranged by size:
4060 2398 858
EaeI y'GGCC_r
Cuts at: 509 1224 1391 3353 3551 4023 4901 5075 5466
Size: 715 167 1962 198 472 878 174 391
Cuts at: 5466 5493 6838 509
Size: 27 1345 987
Fragments arranged by size:
1962 1345 987 878 715 472 391 198
174 167 27
EagI C'GGCC_G
Cuts at: 4901 4901
Size: 7316
EarI CTCTTCn'nnn_
Cuts at: 593 916 1273 2807 2812 3650 5339 5549 593
Size: 323 357 1534 5 838 1689 210 2360
Fragments arranged by size:
2360 1689 1534 838 357 323 210 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1147 5524 5834 5980 7126 1147
Size: 4377 310 146 1146 1337
Fragments arranged by size:
4377 1337 1146 310 146
EcoICRI GAG'CTC
Cuts at: 256 2952 3023 3573 4328 256
Size: 2696 71 550 755 3244
Fragments arranged by size:
3244 2696 755 550 71
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 601 622 745 974 1667 1737 2434 2821 3870
Size: 21 123 229 693 70 697 387 1049
Cuts at: 3870 5141 5573 6320 6588 601
Size: 1271 432 747 268 1329
Fragments arranged by size:
1329 1271 1049 747 697 693 432 387
268 229 123 70 21
EcoO109I rG'GnC_Cy
Cuts at: 262 263 1490 2118 2748 2749 2796 2835 3414
Size: 1 1227 628 630 1 47 39 579
Cuts at: 3414 4455 262
Size: 1041 3123
Fragments arranged by size:
3123 1227 1041 630 628 579 47 39
1 1
EcoRI G'AATT_C
Cuts at: 355 2080 4320 355
Size: 1725 2240 3351
Fragments arranged by size:
3351 2240 1725
EcoRV GAT'ATC
Cuts at: 3615 3615
Size: 7316
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2203 3579 5335 2203
Size: 1376 1756 4184
Fragments arranged by size:
4184 1756 1376
FspI TGC'GCA
Cuts at: 5097 5097
Size: 7316
HaeII r_GCGC'y
Cuts at: 2421 2964 4048 4750 4998 6020 6999 2421
Size: 543 1084 702 248 1022 979 2738
Fragments arranged by size:
2738 1084 1022 979 702 543 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 187 219 1222 1254 1552 1584 1874 1906 2247
Size: 32 1003 32 298 32 290 32 341
Cuts at: 2247 2279 2811 2843 2985 3017 3378 3410 3853
Size: 32 532 32 142 32 361 32 443
Cuts at: 3853 3885 5158 5188 5190 5220 187
Size: 32 1273 30 2 30 2283
Fragments arranged by size:
2283 1273 1003 532 443 361 341 298
290 142 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1236 4355 1236
Size: 3119 4197
HindIII A'AGCT_T
Cuts at: 1426 3970 1426
Size: 2544 4772
KasI G'GCGC_C
Cuts at: 2960 4746 4994 2960
Size: 1786 248 5282
Fragments arranged by size:
5282 1786 248
KpnI G_GTAC'C
Cuts at: 2113 4083 4336 2113
Size: 1970 253 5093
Fragments arranged by size:
5093 1970 253
MluI A'CGCG_T
Cuts at: 6749 6749
Size: 7316
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 623 1895 3101 5987 6171 623
Size: 1272 1206 2886 184 1768
Fragments arranged by size:
2886 1768 1272 1206 184
MscI TGG'CCA
Cuts at: 511 1226 1393 3355 3553 4025 5077 511
Size: 715 167 1962 198 472 1052 2750
Fragments arranged by size:
2750 1962 1052 715 472 198 167
MslI CAynn'nnrTG
Cuts at: 449 844 1085 1226 2494 3398 3673 5305 5432
Size: 395 241 141 1268 904 275 1632 127
Cuts at: 5432 449
Size: 2333
Fragments arranged by size:
2333 1632 1268 904 395 275 241 141
127
MspA1I CmG'CkG
Cuts at: 1386 2370 2919 3214 3442 3744 3804 4427 4741
Size: 984 549 295 228 302 60 623 314
Cuts at: 4741 5101 6114 6359 1386
Size: 360 1013 245 2343
Fragments arranged by size:
2343 1013 984 623 549 360 314 302
295 245 228 60
NaeI GCC'GGC
Cuts at: 2959 5497 2959
Size: 2538 4778
NarI GG'CG_CC
Cuts at: 2961 4747 4995 2961
Size: 1786 248 5282
Fragments arranged by size:
5282 1786 248
NcoI C'CATG_G
Cuts at: 107 506 579 1127 1221 3035 107
Size: 399 73 548 94 1814 4388
Fragments arranged by size:
4388 1814 548 399 94 73
NdeI CA'TA_TG
Cuts at: 815 815
Size: 7316
NgoMIV G'CCGG_C
Cuts at: 2957 5495 2957
Size: 2538 4778
NspI r_CATG'y
Cuts at: 1065 4369 4869 5776 1065
Size: 3304 500 907 2605
Fragments arranged by size:
3304 2605 907 500
PasI CC'CwG_GG
Cuts at: 1599 1599
Size: 7316
PciI A'CATG_T
Cuts at: 5772 5772
Size: 7316
PflMI CCAn_nnn'nTGG
Cuts at: 1203 1320 1389 4069 4089 1203
Size: 117 69 2680 20 4430
Fragments arranged by size:
4430 2680 117 69 20
PfoI T'CCnGG_A
Cuts at: 404 488 1736 1999 2174 2972 3293 3410 404
Size: 84 1248 263 175 798 321 117 4310
Fragments arranged by size:
4310 1248 798 321 263 175 117 84
PmeI GTTT'AAAC
Cuts at: 4315 4315
Size: 7316
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6455 6487 6455
Size: 32 7284
PpuMI rG'GwC_Cy
Cuts at: 2835 3414 2835
Size: 579 6737
PshAI GACnn'nnGTC
Cuts at: 650 650
Size: 7316
PsiI TTA'TAA
Cuts at: 809 809
Size: 7316
PspOMI G'GGCC_C
Cuts at: 262 1490 2748 262
Size: 1228 1258 4830
Fragments arranged by size:
4830 1258 1228
PstI C_TGCA'G
Cuts at: 281 854 1148 1460 1509 1521 1580 1982 2915
Size: 573 294 312 49 12 59 402 933
Cuts at: 2915 3213 4363 5048 281
Size: 298 1150 685 2549
Fragments arranged by size:
2549 1150 933 685 573 402 312 298
294 59 49 12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7316
PvuII CAG'CTG
Cuts at: 1386 2370 2919 3214 3804 4741 5101 1386
Size: 984 549 295 590 937 360 3601
Fragments arranged by size:
3601 984 937 590 549 360 295
RsrII CG'GwC_CG
Cuts at: 5511 5511
Size: 7316
SacI G_AGCT'C
Cuts at: 258 2954 3025 3575 4330 258
Size: 2696 71 550 755 3244
Fragments arranged by size:
3244 2696 755 550 71
SalI G'TCGA_C
Cuts at: 4353 4353
Size: 7316
SanDI GG'GwC_CC
Cuts at: 3414 3414
Size: 7316
SapI GCTCTTCn'nnn_
Cuts at: 916 1273 3650 5339 5549 916
Size: 357 2377 1689 210 2683
Fragments arranged by size:
2683 2377 1689 357 210
SbfI CC_TGCA'GG
Cuts at: 4363 4363
Size: 7316
ScaI AGT'ACT
Cuts at: 6833 6833
Size: 7316
SfcI C'TryA_G
Cuts at: 33 277 297 827 850 1144 1456 1505 1517
Size: 244 20 530 23 294 312 49 12
Cuts at: 1517 1576 1588 1862 1978 1989 2057 2532 2911
Size: 59 12 274 116 11 68 475 379
Cuts at: 2911 3189 3209 4097 4359 5044 6037 6228 33
Size: 278 20 888 262 685 993 191 1121
Fragments arranged by size:
1121 993 888 685 530 475 379 312
294 278 274 262 244 191 116 68
59 49 23 20 20 12 12 11
SfoI GGC'GCC
Cuts at: 2962 4748 4996 2962
Size: 1786 248 5282
Fragments arranged by size:
5282 1786 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7316
SmaI CCC'GGG
Cuts at: 1855 4338 1855
Size: 2483 4833
SmlI C'TyrA_G
Cuts at: 1715 2311 3469 4015 4203 4471 5386 5878 6140
Size: 596 1158 546 188 268 915 492 262
Cuts at: 6140 1715
Size: 2891
Fragments arranged by size:
2891 1158 915 596 546 492 268 262
188
SpeI A'CTAG_T
Cuts at: 67 1947 67
Size: 1880 5436
SphI G_CATG'C
Cuts at: 4369 4869 4369
Size: 500 6816
StuI AGG'CCT
Cuts at: 3185 3824 3185
Size: 639 6677
StyI C'CwwG_G
Cuts at: 107 506 540 569 579 1127 1221 2240 3005
Size: 399 34 29 10 548 94 1019 765
Cuts at: 3005 3035 3228 3701 4450 4804 107
Size: 30 193 473 749 354 2619
Fragments arranged by size:
2619 1019 765 749 548 473 399 354
193 94 34 30 29 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2699 4177 4275 4899 5032 5491 5700 2699
Size: 1478 98 624 133 459 209 4315
Fragments arranged by size:
4315 1478 624 459 209 133 98
TatI w'GTAC_w
Cuts at: 876 6831 876
Size: 5955 1361
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2684 2716 3721 3753 6833 6865 2684
Size: 32 1005 32 3080 32 3135
Fragments arranged by size:
3135 3080 1005 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4117 5113 4117
Size: 996 6320
XbaI T'CTAG_A
Cuts at: 4347 4347
Size: 7316
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1134 1386 1538 2122 2693 3348 4811 1134
Size: 252 152 584 571 655 1463 3639
Fragments arranged by size:
3639 1463 655 584 571 252 152
XhoI C'TCGA_G
Cuts at: 1715 1715
Size: 7316
XmaI C'CCGG_G
Cuts at: 1853 4336 1853
Size: 2483 4833
XmnI GAAnn'nnTTC
Cuts at: 2589 3562 2589
Size: 973 6343
ZraI GAC'GTC
Cuts at: 1342 1342
Size: 7316
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AscI AvrII BaeI BbvCI BfrBI BglI BmtI
BsiWI BsrGI BssHII BstEII BstZ17I ClaI DraIII EcoNI
FseI FspAI HpaI MfeI NheI NotI NruI NsiI
PacI PmlI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI