Restriction map for
pF1KA0160
(Circular) (Six-base) MAPSORT of: pF1KA0160.seq Check: 7967 from: 1 to: 5330
pF1KA0160 5330 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 732 732
Size: 5330
AccI GT'mk_AC
Cuts at: 2368 2368
Size: 5330
Acc65I G'GTAC_C
Cuts at: 2346 2346
Size: 5330
AclI AA'CG_TT
Cuts at: 5170 5170
Size: 5330
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1454 1766 2234 3155 3587 4334 4602 1454
Size: 312 468 921 432 747 268 2182
Fragments arranged by size:
2182 921 747 468 432 312 268
AflIII A'CryG_T
Cuts at: 458 2682 3786 4763 458
Size: 2224 1104 977 1025
Fragments arranged by size:
2224 1104 1025 977
AgeI A'CCGG_T
Cuts at: 315 3718 315
Size: 3403 1927
AleI CACnn'nnGTG
Cuts at: 128 128
Size: 5330
AlwNI CAG_nnn'CTG
Cuts at: 2635 4202 2635
Size: 1567 3763
ApaI G_GGCC'C
Cuts at: 155 155
Size: 5330
ApaLI G'TGCA_C
Cuts at: 4100 4100
Size: 5330
ApoI r'AATT_y
Cuts at: 645 783 814 1248 1732 2058 2334 4683 4939
Size: 138 31 434 484 326 276 2349 256
Cuts at: 4939 645
Size: 1036
Fragments arranged by size:
2349 1036 484 434 326 276 256 138
31
AseI AT'TA_AT
Cuts at: 20 905 20
Size: 885 4445
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5330
AvaI C'yCGr_G
Cuts at: 147 2350 147
Size: 2203 3127
BamHI G'GATC_C
Cuts at: 1869 2355 1869
Size: 486 4844
BanI G'GyrC_C
Cuts at: 111 744 2346 2760 3008 3043 111
Size: 633 1602 414 248 35 2398
Fragments arranged by size:
2398 1602 633 414 248 35
BanII G_rGCy'C
Cuts at: 155 2344 3374 155
Size: 2189 1030 2111
Fragments arranged by size:
2189 2111 1030
BbeI G_GCGC'C
Cuts at: 115 2764 3012 115
Size: 2649 248 2433
Fragments arranged by size:
2649 2433 248
BciVI GTATCCnnnnn_n'
Cuts at: 1350 3228 3989 5330 1350
Size: 1878 761 1341 1350
Fragments arranged by size:
1878 1350 1341 761
BclI T'GATC_A
Cuts at: 2850 2850
Size: 5330
BfrBI ATG'CAT
Cuts at: 2097 2097
Size: 5330
BglII A'GATC_T
Cuts at: 392 1332 2845 4882 392
Size: 940 1513 2037 840
Fragments arranged by size:
2037 1513 940 840
BlpI GC'TnA_GC
Cuts at: 2442 2442
Size: 5330
Bme1580I G_kGCm'C
Cuts at: 155 2955 3048 4104 155
Size: 2800 93 1056 1381
Fragments arranged by size:
2800 1381 1056 93
BmgBI CAC'GTC
Cuts at: 342 342
Size: 5330
BmrI ACTGGGnnnn_n'
Cuts at: 2542 2623 2958 5102 2542
Size: 81 335 2144 2770
Fragments arranged by size:
2770 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1154 3697 1154
Size: 2543 2787
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 459 352
Size: 107 5223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1483 2506 3385 3877 4175 1483
Size: 1023 879 492 298 2638
Fragments arranged by size:
2638 1023 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1192 5034 1192
Size: 3842 1488
BsaAI yAC'GTr
Cuts at: 2683 2683
Size: 5330
BsaBI GATnn'nnATC
Cuts at: 6 1625 2844 4881 6
Size: 1619 1219 2037 455
Fragments arranged by size:
2037 1619 1219 455
BsaHI Gr'CG_yC
Cuts at: 112 2761 3009 3711 5207 112
Size: 2649 248 702 1496 235
Fragments arranged by size:
2649 1496 702 248 235
BsaWI w'CCGG_w
Cuts at: 315 2519 3040 3718 3992 4139 4550 315
Size: 2204 521 678 274 147 411 1095
Fragments arranged by size:
2204 1095 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 253 283 253
Size: 30 5300
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 261 264 267 270 261
Size: 3 3 3 5321
Fragments arranged by size:
5321 3 3 3
BseYI C'CCAG_C
Cuts at: 154 2755 3513 4090 4586 154
Size: 2601 758 577 496 898
Fragments arranged by size:
2601 898 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1719 1719
Size: 5330
BsiEI CG_ry'CG
Cuts at: 104 2872 2918 4126 104
Size: 2768 46 1208 1308
Fragments arranged by size:
2768 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 341 2344 3122 3312 4104 341
Size: 2003 778 190 792 1567
Fragments arranged by size:
2003 1567 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 155 341 2344 2955 3048 3122 3312 3374 4104
Size: 186 2003 611 93 74 190 62 730
Cuts at: 4104 155
Size: 1381
Fragments arranged by size:
2003 1381 730 611 190 186 93 74
62
BspEI T'CCGG_A
Cuts at: 4550 4550
Size: 5330
BspHI T'CATG_A
Cuts at: 1970 4506 1970
Size: 2536 2794
BspMI ACCTGCnnnn'nnnn_
Cuts at: 732 1457 1825 2380 2594 2896 3277 732
Size: 725 368 555 214 302 381 2785
Fragments arranged by size:
2785 725 555 381 368 302 214
BsrBI CCG'CTC
Cuts at: 50 3622 3676 5160 5325 50
Size: 3572 54 1484 165 55
Fragments arranged by size:
3572 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1452 3242 1452
Size: 1790 3540
BsrFI r'CCGG_y
Cuts at: 315 3328 3509 3718 315
Size: 3013 181 209 1927
Fragments arranged by size:
3013 1927 209 181
BssSI C'ACGA_G
Cuts at: 355 3601 3959 355
Size: 3246 358 1726
Fragments arranged by size:
3246 1726 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2707 2707
Size: 5330
BstBI TT'CG_AA
Cuts at: 408 1145 3691 408
Size: 737 2546 2047
Fragments arranged by size:
2546 2047 737
BstYI r'GATC_y
Cuts at: 1 392 1332 1869 2355 2822 2845 3180 3426
Size: 391 940 537 486 467 23 335 246
Cuts at: 3426 4438 4524 4536 4882 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 940 537 486 467 449 391 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1616 1616
Size: 5330
BtgI C'CryG_G
Cuts at: 107 284 107
Size: 177 5153
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1612 3280 3461 3624 1612
Size: 1668 181 163 3318
Fragments arranged by size:
3318 1668 181 163
BtsI GCAGTG_nn'
Cuts at: 2573 2573
Size: 5330
DraI TTT'AAA
Cuts at: 504 2329 504
Size: 1825 3505
DrdI GACnn_nn'nnGTC
Cuts at: 3036 3894 3036
Size: 858 4472
EaeI y'GGCC_r
Cuts at: 1785 2915 3089 3480 3507 4852 1785
Size: 1130 174 391 27 1345 2263
Fragments arranged by size:
2263 1345 1130 391 174 27
EagI C'GGCC_G
Cuts at: 2915 2915
Size: 5330
EarI CTCTTCn'nnn_
Cuts at: 879 935 1123 1187 1904 3353 3563 879
Size: 56 188 64 717 1449 210 2646
Fragments arranged by size:
2646 1449 717 210 188 64 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3538 3848 3994 5140 3538
Size: 310 146 1146 3728
Fragments arranged by size:
3728 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2342 2342
Size: 5330
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 459 1454 1766 2234 3155 3587 4334 4602
Size: 107 995 312 468 921 432 747 268
Cuts at: 4602 352
Size: 1080
Fragments arranged by size:
1080 995 921 747 468 432 312 268
107
EcoO109I rG'GnC_Cy
Cuts at: 151 1162 2469 151
Size: 1011 1307 3012
Fragments arranged by size:
3012 1307 1011
EcoRI G'AATT_C
Cuts at: 2334 2334
Size: 5330
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 685 3349 685
Size: 2664 2666
FspI TGC'GCA
Cuts at: 3111 3111
Size: 5330
HaeII r_GCGC'y
Cuts at: 115 1029 2764 3012 4034 5013 115
Size: 914 1735 248 1022 979 432
Fragments arranged by size:
1735 1022 979 914 432 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 505 537 538 570 604 636 1248 1280 1317
Size: 32 1 32 34 32 612 32 37
Cuts at: 1317 1349 2272 2304 3172 3202 3204 3234 505
Size: 32 923 32 868 30 2 30 2601
Fragments arranged by size:
2601 923 868 612 37 34 32 32
32 32 32 32 30 30 2 1
HincII GTy'rAC
Cuts at: 2369 2369
Size: 5330
HindIII A'AGCT_T
Cuts at: 2267 2267
Size: 5330
KasI G'GCGC_C
Cuts at: 111 2760 3008 111
Size: 2649 248 2433
Fragments arranged by size:
2649 2433 248
KpnI G_GTAC'C
Cuts at: 2350 2350
Size: 5330
MluI A'CGCG_T
Cuts at: 4763 4763
Size: 5330
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1168 4001 4185 1168
Size: 2833 184 2313
Fragments arranged by size:
2833 2313 184
MscI TGG'CCA
Cuts at: 1787 3091 1787
Size: 1304 4026
MslI CAynn'nnrTG
Cuts at: 128 608 3319 3446 128
Size: 480 2711 127 2012
Fragments arranged by size:
2711 2012 480 127
MspA1I CmG'CkG
Cuts at: 286 295 2441 2755 3115 4128 4373 286
Size: 9 2146 314 360 1013 245 1243
Fragments arranged by size:
2146 1243 1013 360 314 245 9
NaeI GCC'GGC
Cuts at: 3511 3511
Size: 5330
NarI GG'CG_CC
Cuts at: 112 2761 3009 112
Size: 2649 248 2433
Fragments arranged by size:
2649 2433 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5330
NdeI CA'TA_TG
Cuts at: 836 836
Size: 5330
NgoMIV G'CCGG_C
Cuts at: 3509 3509
Size: 5330
NsiI A_TGCA'T
Cuts at: 2099 2099
Size: 5330
NspI r_CATG'y
Cuts at: 462 1728 2097 2383 2883 3790 462
Size: 1266 369 286 500 907 2002
Fragments arranged by size:
2002 1266 907 500 369 286
PciI A'CATG_T
Cuts at: 458 3786 458
Size: 3328 2002
PfoI T'CCnGG_A
Cuts at: 876 876
Size: 5330
PmeI GTTT'AAAC
Cuts at: 2329 2329
Size: 5330
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4469 4501 4469
Size: 32 5298
PpuMI rG'GwC_Cy
Cuts at: 1162 1162
Size: 5330
PspOMI G'GGCC_C
Cuts at: 151 151
Size: 5330
PstI C_TGCA'G
Cuts at: 2377 3062 2377
Size: 685 4645
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5330
PvuII CAG'CTG
Cuts at: 2755 3115 2755
Size: 360 4970
RsrII CG'GwC_CG
Cuts at: 3525 3525
Size: 5330
SacI G_AGCT'C
Cuts at: 2344 2344
Size: 5330
SacII CC_GC'GG
Cuts at: 287 287
Size: 5330
SalI G'TCGA_C
Cuts at: 2367 2367
Size: 5330
SapI GCTCTTCn'nnn_
Cuts at: 935 3353 3563 935
Size: 2418 210 2702
Fragments arranged by size:
2702 2418 210
SbfI CC_TGCA'GG
Cuts at: 2377 2377
Size: 5330
ScaI AGT'ACT
Cuts at: 4847 4847
Size: 5330
SfcI C'TryA_G
Cuts at: 33 396 1328 2373 3058 4051 4242 33
Size: 363 932 1045 685 993 191 1121
Fragments arranged by size:
1121 1045 993 932 685 363 191
SfoI GGC'GCC
Cuts at: 113 2762 3010 113
Size: 2649 248 2433
Fragments arranged by size:
2649 2433 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5330
SmaI CCC'GGG
Cuts at: 2352 2352
Size: 5330
SmlI C'TyrA_G
Cuts at: 1498 2485 3400 3892 4154 1498
Size: 987 915 492 262 2674
Fragments arranged by size:
2674 987 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5330
SphI G_CATG'C
Cuts at: 2097 2383 2883 2097
Size: 286 500 4544
Fragments arranged by size:
4544 500 286
SspI AAT'ATT
Cuts at: 434 927 1391 434
Size: 493 464 4373
Fragments arranged by size:
4373 493 464
StuI AGG'CCT
Cuts at: 373 373
Size: 5330
StyI C'CwwG_G
Cuts at: 107 1459 2464 2818 107
Size: 1352 1005 354 2619
Fragments arranged by size:
2619 1352 1005 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2913 3046 3505 3714 2913
Size: 133 459 209 4529
Fragments arranged by size:
4529 459 209 133
TatI w'GTAC_w
Cuts at: 1465 4845 1465
Size: 3380 1950
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4847 4879 4847
Size: 32 5298
Tth111I GACn'n_nGTC
Cuts at: 3127 3127
Size: 5330
XbaI T'CTAG_A
Cuts at: 2361 2361
Size: 5330
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1068 2825 1068
Size: 1757 3573
XmaI C'CCGG_G
Cuts at: 2350 2350
Size: 5330
XmnI GAAnn'nnTTC
Cuts at: 944 944
Size: 5330
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PciI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BglI BmtI Bpu10I BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI
NheI NotI NruI PacI PasI PflMI PmlI PshAI
PsiI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI SwaI XhoI ZraI