Restriction map for
pF1KA0164
(Circular) (Six-base) MAPSORT of: pF1KA0164.seq Check: 262 from: 1 to: 5873
pF1KA0164 5873 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AccI GT'mk_AC
Cuts at: 1681 2911 1681
Size: 1230 4643
Acc65I G'GTAC_C
Cuts at: 2889 2889
Size: 5873
AclI AA'CG_TT
Cuts at: 5713 5713
Size: 5873
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 635 1242 1671 1763 1895 2045 2354 3698 4130
Size: 607 429 92 132 150 309 1344 432
Cuts at: 4130 4877 5145 635
Size: 747 268 1363
Fragments arranged by size:
1363 1344 747 607 432 429 309 268
150 132 92
AflIII A'CryG_T
Cuts at: 3225 4329 5306 3225
Size: 1104 977 3792
Fragments arranged by size:
3792 1104 977
AgeI A'CCGG_T
Cuts at: 4261 4261
Size: 5873
AlwNI CAG_nnn'CTG
Cuts at: 3178 4745 3178
Size: 1567 4306
ApaLI G'TGCA_C
Cuts at: 4643 4643
Size: 5873
ApoI r'AATT_y
Cuts at: 709 1557 1858 2434 2877 5226 5482 709
Size: 848 301 576 443 2349 256 1100
Fragments arranged by size:
2349 1100 848 576 443 301 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5873
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5873
AvaI C'yCGr_G
Cuts at: 2396 2893 2396
Size: 497 5376
BamHI G'GATC_C
Cuts at: 2613 2898 2613
Size: 285 5588
BanI G'GyrC_C
Cuts at: 1761 2889 3303 3551 3586 1761
Size: 1128 414 248 35 4048
Fragments arranged by size:
4048 1128 414 248 35
BanII G_rGCy'C
Cuts at: 2053 2248 2887 3917 2053
Size: 195 639 1030 4009
Fragments arranged by size:
4009 1030 639 195
BbeI G_GCGC'C
Cuts at: 3307 3555 3307
Size: 248 5625
BbsI GAAGACnn'nnnn_
Cuts at: 1556 1556
Size: 5873
BciVI GTATCCnnnnn_n'
Cuts at: 3771 4532 5873 3771
Size: 761 1341 3771
Fragments arranged by size:
3771 1341 761
BclI T'GATC_A
Cuts at: 3393 3393
Size: 5873
BglII A'GATC_T
Cuts at: 184 490 508 3388 5425 184
Size: 306 18 2880 2037 632
Fragments arranged by size:
2880 2037 632 306 18
BlpI GC'TnA_GC
Cuts at: 2985 2985
Size: 5873
Bme1580I G_kGCm'C
Cuts at: 3498 3591 4647 3498
Size: 93 1056 4724
Fragments arranged by size:
4724 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1115 2384 3085 3166 3501 5645 1115
Size: 1269 701 81 335 2144 1343
Fragments arranged by size:
2144 1343 1269 701 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1748 4240 1748
Size: 2492 3381
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2232 2232
Size: 5873
Bpu10I CC'TnA_GC
Cuts at: 1598 1598
Size: 5873
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1399 3049 3928 4420 4718 1399
Size: 1650 879 492 298 2554
Fragments arranged by size:
2554 1650 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 224 248 524 593 2586 2809 5577 224
Size: 24 276 69 1993 223 2768 520
Fragments arranged by size:
2768 1993 520 276 223 69 24
BsaAI yAC'GTr
Cuts at: 3226 3226
Size: 5873
BsaBI GATnn'nnATC
Cuts at: 6 177 183 2361 3387 5424 6
Size: 171 6 2178 1026 2037 455
Fragments arranged by size:
2178 2037 1026 455 171 6
BsaHI Gr'CG_yC
Cuts at: 3304 3552 4254 5750 3304
Size: 248 702 1496 3427
Fragments arranged by size:
3427 1496 702 248
BsaWI w'CCGG_w
Cuts at: 733 1391 3062 3583 4261 4535 4682 5093 733
Size: 658 1671 521 678 274 147 411 1513
Fragments arranged by size:
1671 1513 678 658 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 371 374 1330 1417 2549 371
Size: 3 956 87 1132 3695
Fragments arranged by size:
3695 1132 956 87 3
BseYI C'CCAG_C
Cuts at: 2545 3298 4056 4633 5129 2545
Size: 753 758 577 496 3289
Fragments arranged by size:
3289 758 753 577 496
BsiEI CG_ry'CG
Cuts at: 104 3415 3461 4669 104
Size: 3311 46 1208 1308
Fragments arranged by size:
3311 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2887 3665 3855 4647 2887
Size: 778 190 792 4113
Fragments arranged by size:
4113 792 778 190
BsmI GAATG_Cn'
Cuts at: 888 1038 888
Size: 150 5723
BsmBI CGTCTCn'nnnn_
Cuts at: 303 303
Size: 5873
Bsp1286I G_dGCh'C
Cuts at: 2053 2248 2887 3498 3591 3665 3855 3917 4647
Size: 195 639 611 93 74 190 62 730
Cuts at: 4647 2053
Size: 3279
Fragments arranged by size:
3279 730 639 611 195 190 93 74
62
BspEI T'CCGG_A
Cuts at: 5093 5093
Size: 5873
BspHI T'CATG_A
Cuts at: 2772 5049 2772
Size: 2277 3596
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1593 1800 2923 3137 3439 3820 1593
Size: 207 1123 214 302 381 3646
Fragments arranged by size:
3646 1123 381 302 214 207
BsrBI CCG'CTC
Cuts at: 50 1998 4165 4219 5703 5868 50
Size: 1948 2167 54 1484 165 55
Fragments arranged by size:
2167 1948 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1988 3785 1988
Size: 1797 4076
BsrFI r'CCGG_y
Cuts at: 497 3871 4052 4261 497
Size: 3374 181 209 2109
Fragments arranged by size:
3374 2109 209 181
BssSI C'ACGA_G
Cuts at: 1670 2492 4144 4502 1670
Size: 822 1652 358 3041
Fragments arranged by size:
3041 1652 822 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3250 3250
Size: 5873
BstBI TT'CG_AA
Cuts at: 2508 4234 2508
Size: 1726 4147
BstXI CCAn_nnnn'nTGG
Cuts at: 2791 2791
Size: 5873
BstYI r'GATC_y
Cuts at: 1 184 460 478 490 508 946 2613 2898
Size: 183 276 18 12 18 438 1667 285
Cuts at: 2898 3365 3388 3723 3969 4981 5067 5079 5425
Size: 467 23 335 246 1012 86 12 346
Cuts at: 5425 1
Size: 449
Fragments arranged by size:
1667 1012 467 449 438 346 335 285
276 246 183 86 23 18 18 12
12
Bsu36I CC'TnA_GG
Cuts at: 2092 2092
Size: 5873
BtgI C'CryG_G
Cuts at: 107 2819 107
Size: 2712 3161
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3823 4004 4167 3823
Size: 181 163 5529
Fragments arranged by size:
5529 181 163
BtsI GCAGTG_nn'
Cuts at: 3116 3116
Size: 5873
ClaI AT'CG_AT
Cuts at: 704 704
Size: 5873
DraI TTT'AAA
Cuts at: 2133 2439 2872 2133
Size: 306 433 5134
Fragments arranged by size:
5134 433 306
DraIII CAC_nnn'GTG
Cuts at: 1935 1935
Size: 5873
DrdI GACnn_nn'nnGTC
Cuts at: 3579 4437 3579
Size: 858 5015
EaeI y'GGCC_r
Cuts at: 3458 3632 4023 4050 5395 3458
Size: 174 391 27 1345 3936
Fragments arranged by size:
3936 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3458 3458
Size: 5873
EarI CTCTTCn'nnn_
Cuts at: 540 626 1130 2114 2373 2520 2597 2691 2807
Size: 86 504 984 259 147 77 94 116
Cuts at: 2807 3896 4106 540
Size: 1089 210 2307
Fragments arranged by size:
2307 1089 984 504 259 210 147 116
94 86 77
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4081 4391 4537 5683 4081
Size: 310 146 1146 4271
Fragments arranged by size:
4271 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2885 2885
Size: 5873
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 635 1242 1671 1763 1895 2045 2232 2354 3698
Size: 607 429 92 132 150 187 122 1344
Cuts at: 3698 4130 4877 5145 635
Size: 432 747 268 1363
Fragments arranged by size:
1363 1344 747 607 432 429 268 187
150 132 122 92
EcoNI CCTnn'n_nnAGG
Cuts at: 425 425
Size: 5873
EcoO109I rG'GnC_Cy
Cuts at: 3012 3012
Size: 5873
EcoRI G'AATT_C
Cuts at: 1858 2877 1858
Size: 1019 4854
EcoRV GAT'ATC
Cuts at: 370 370
Size: 5873
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1701 2661 2664 3892 1701
Size: 960 3 1228 3682
Fragments arranged by size:
3682 1228 960 3
FspI TGC'GCA
Cuts at: 3654 3654
Size: 5873
HaeII r_GCGC'y
Cuts at: 2733 3307 3555 4577 5556 2733
Size: 574 248 1022 979 3050
Fragments arranged by size:
3050 1022 979 574 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1123 1155 1186 1218 1255 1287 3715 3745 3747
Size: 32 31 32 37 32 2428 30 2
Cuts at: 3747 3777 1123
Size: 30 3219
Fragments arranged by size:
3219 2428 37 32 32 32 31 30
30 2
HincII GTy'rAC
Cuts at: 2912 2912
Size: 5873
KasI G'GCGC_C
Cuts at: 3303 3551 3303
Size: 248 5625
KpnI G_GTAC'C
Cuts at: 2893 2893
Size: 5873
MluI A'CGCG_T
Cuts at: 5306 5306
Size: 5873
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 918 4544 4728 918
Size: 3626 184 2063
Fragments arranged by size:
3626 2063 184
MscI TGG'CCA
Cuts at: 3634 3634
Size: 5873
MslI CAynn'nnrTG
Cuts at: 377 3862 3989 377
Size: 3485 127 2261
Fragments arranged by size:
3485 2261 127
MspA1I CmG'CkG
Cuts at: 1978 2984 3298 3658 4671 4916 1978
Size: 1006 314 360 1013 245 2935
Fragments arranged by size:
2935 1013 1006 360 314 245
NaeI GCC'GGC
Cuts at: 4054 4054
Size: 5873
NarI GG'CG_CC
Cuts at: 3304 3552 3304
Size: 248 5625
NcoI C'CATG_G
Cuts at: 107 2819 107
Size: 2712 3161
NgoMIV G'CCGG_C
Cuts at: 4052 4052
Size: 5873
NruI TCG'CGA
Cuts at: 284 284
Size: 5873
NspI r_CATG'y
Cuts at: 2926 3426 4333 2926
Size: 500 907 4466
Fragments arranged by size:
4466 907 500
PciI A'CATG_T
Cuts at: 4329 4329
Size: 5873
PfoI T'CCnGG_A
Cuts at: 442 442
Size: 5873
PmeI GTTT'AAAC
Cuts at: 2872 2872
Size: 5873
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5012 5044 5012
Size: 32 5841
PshAI GACnn'nnGTC
Cuts at: 585 585
Size: 5873
PsiI TTA'TAA
Cuts at: 1484 1484
Size: 5873
PstI C_TGCA'G
Cuts at: 2920 3605 2920
Size: 685 5188
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5873
PvuII CAG'CTG
Cuts at: 1978 3298 3658 1978
Size: 1320 360 4193
Fragments arranged by size:
4193 1320 360
RsrII CG'GwC_CG
Cuts at: 4068 4068
Size: 5873
SacI G_AGCT'C
Cuts at: 2887 2887
Size: 5873
SalI G'TCGA_C
Cuts at: 2910 2910
Size: 5873
SapI GCTCTTCn'nnn_
Cuts at: 2520 3896 4106 2520
Size: 1376 210 4287
Fragments arranged by size:
4287 1376 210
SbfI CC_TGCA'GG
Cuts at: 2920 2920
Size: 5873
ScaI AGT'ACT
Cuts at: 2037 2644 5390 2037
Size: 607 2746 2520
Fragments arranged by size:
2746 2520 607
SfcI C'TryA_G
Cuts at: 33 2916 3601 4594 4785 33
Size: 2883 685 993 191 1121
Fragments arranged by size:
2883 1121 993 685 191
SfoI GGC'GCC
Cuts at: 3305 3553 3305
Size: 248 5625
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5873
SmaI CCC'GGG
Cuts at: 2895 2895
Size: 5873
SmlI C'TyrA_G
Cuts at: 1414 2396 3028 3943 4435 4697 1414
Size: 982 632 915 492 262 2590
Fragments arranged by size:
2590 982 915 632 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5873
SphI G_CATG'C
Cuts at: 2926 3426 2926
Size: 500 5373
StuI AGG'CCT
Cuts at: 1518 1518
Size: 5873
StyI C'CwwG_G
Cuts at: 107 1940 2240 2784 2819 3007 3361 107
Size: 1833 300 544 35 188 354 2619
Fragments arranged by size:
2619 1833 544 354 300 188 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2610 3456 3589 4048 4257 2610
Size: 846 133 459 209 4226
Fragments arranged by size:
4226 846 459 209 133
TatI w'GTAC_w
Cuts at: 853 1418 1825 2035 2642 5388 853
Size: 565 407 210 607 2746 1338
Fragments arranged by size:
2746 1338 607 565 407 210
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1493 1525 1921 1953 5390 5422 1493
Size: 32 396 32 3437 32 1944
Fragments arranged by size:
3437 1944 396 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 428 3670 428
Size: 3242 2631
XbaI T'CTAG_A
Cuts at: 124 151 193 268 1141 1248 2904 124
Size: 27 42 75 873 107 1656 3093
Fragments arranged by size:
3093 1656 873 107 75 42 27
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3368 3368
Size: 5873
XhoI C'TCGA_G
Cuts at: 2396 2396
Size: 5873
XmaI C'CCGG_G
Cuts at: 2893 2893
Size: 5873
XmnI GAAnn'nnTTC
Cuts at: 234 2644 234
Size: 2410 3463
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI NspI PciI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BsaXI BsgI BsiWI BsrGI BssHII BstEII
BstZ17I CspCI FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NsiI PacI PasI PflMI PmlI PpuMI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI ZraI