Restriction map for
pF1KA0175
(Circular) (Six-base) MAPSORT of: pF1KA0175.seq Check: 9247 from: 1 to: 5066
pF1KA0175 5066 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 831 1362 831
Size: 531 4535
AccI GT'mk_AC
Cuts at: 2104 2104
Size: 5066
Acc65I G'GTAC_C
Cuts at: 2082 2082
Size: 5066
AclI AA'CG_TT
Cuts at: 4906 4906
Size: 5066
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1915 2891 3323 4070 4338 1915
Size: 976 432 747 268 2643
Fragments arranged by size:
2643 976 747 432 268
AflII C'TTAA_G
Cuts at: 1999 1999
Size: 5066
AflIII A'CryG_T
Cuts at: 2418 3522 4499 2418
Size: 1104 977 2985
Fragments arranged by size:
2985 1104 977
AgeI A'CCGG_T
Cuts at: 3454 3454
Size: 5066
AhdI GACnn_n'nnGTC
Cuts at: 1158 1158
Size: 5066
AleI CACnn'nnGTG
Cuts at: 1896 1896
Size: 5066
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1079 1111 1079
Size: 32 5034
AlwNI CAG_nnn'CTG
Cuts at: 637 1261 1530 2371 3938 637
Size: 624 269 841 1567 1765
Fragments arranged by size:
1765 1567 841 624 269
ApaI G_GGCC'C
Cuts at: 1769 1769
Size: 5066
ApaLI G'TGCA_C
Cuts at: 475 3836 475
Size: 3361 1705
ApoI r'AATT_y
Cuts at: 516 1560 2070 4419 4675 516
Size: 1044 510 2349 256 907
Fragments arranged by size:
2349 1044 907 510 256
AseI AT'TA_AT
Cuts at: 20 1219 20
Size: 1199 3867
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5066
AvaI C'yCGr_G
Cuts at: 434 1082 2086 434
Size: 648 1004 3414
Fragments arranged by size:
3414 1004 648
BamHI G'GATC_C
Cuts at: 2091 2091
Size: 5066
BanI G'GyrC_C
Cuts at: 1613 2082 2496 2744 2779 1613
Size: 469 414 248 35 3900
Fragments arranged by size:
3900 469 414 248 35
BanII G_rGCy'C
Cuts at: 1769 2080 3110 1769
Size: 311 1030 3725
Fragments arranged by size:
3725 1030 311
BbeI G_GCGC'C
Cuts at: 2500 2748 2500
Size: 248 4818
BbsI GAAGACnn'nnnn_
Cuts at: 435 1779 2042 435
Size: 1344 263 3459
Fragments arranged by size:
3459 1344 263
BciVI GTATCCnnnnn_n'
Cuts at: 2964 3725 5066 2964
Size: 761 1341 2964
Fragments arranged by size:
2964 1341 761
BclI T'GATC_A
Cuts at: 1038 2586 1038
Size: 1548 3518
BglI GCCn_nnn'nGGC
Cuts at: 1078 1078
Size: 5066
BglII A'GATC_T
Cuts at: 2581 4618 2581
Size: 2037 3029
BlpI GC'TnA_GC
Cuts at: 2178 2178
Size: 5066
Bme1580I G_kGCm'C
Cuts at: 479 1769 2691 2784 3840 479
Size: 1290 922 93 1056 1705
Fragments arranged by size:
1705 1290 1056 922 93
BmrI ACTGGGnnnn_n'
Cuts at: 803 2278 2359 2694 4838 803
Size: 1475 81 335 2144 1031
Fragments arranged by size:
2144 1475 1031 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1495 3433 1495
Size: 1938 3128
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 398 1319 1430 227
Size: 171 921 111 3863
Fragments arranged by size:
3863 921 171 111
Bpu10I CC'TnA_GC
Cuts at: 1433 1433
Size: 5066
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 490 2242 3121 3613 3911 385
Size: 105 1752 879 492 298 1540
Fragments arranged by size:
1752 1540 879 492 298 105
BsaI GGTCTCn'nnnn_
Cuts at: 424 568 4770 424
Size: 144 4202 720
Fragments arranged by size:
4202 720 144
BsaAI yAC'GTr
Cuts at: 2419 2419
Size: 5066
BsaBI GATnn'nnATC
Cuts at: 6 533 596 2580 4617 6
Size: 527 63 1984 2037 455
Fragments arranged by size:
2037 1984 527 455 63
BsaHI Gr'CG_yC
Cuts at: 2497 2745 3447 4943 2497
Size: 248 702 1496 2620
Fragments arranged by size:
2620 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2255 2776 3454 3728 3875 4286 2255
Size: 521 678 274 147 411 3035
Fragments arranged by size:
3035 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 200 230 1667 1697 200
Size: 30 1437 30 3569
Fragments arranged by size:
3569 1437 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 395 442 1669 395
Size: 47 1227 3792
Fragments arranged by size:
3792 1227 47
BseYI C'CCAG_C
Cuts at: 2491 3249 3826 4322 2491
Size: 758 577 496 3235
Fragments arranged by size:
3235 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2608 2654 3862 104
Size: 2504 46 1208 1308
Fragments arranged by size:
2504 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 479 1730 2080 2858 3048 3840 479
Size: 1251 350 778 190 792 1705
Fragments arranged by size:
1705 1251 792 778 350 190
BsmI GAATG_Cn'
Cuts at: 815 815
Size: 5066
BsmBI CGTCTCn'nnnn_
Cuts at: 1754 1754
Size: 5066
Bsp1286I G_dGCh'C
Cuts at: 479 1730 1769 2080 2691 2784 2858 3048 3110
Size: 1251 39 311 611 93 74 190 62
Cuts at: 3110 3840 479
Size: 730 1705
Fragments arranged by size:
1705 1251 730 611 311 190 93 74
62 39
BspEI T'CCGG_A
Cuts at: 4286 4286
Size: 5066
BspHI T'CATG_A
Cuts at: 4242 4242
Size: 5066
BspMI ACCTGCnnnn'nnnn_
Cuts at: 831 1362 2116 2330 2632 3013 831
Size: 531 754 214 302 381 2884
Fragments arranged by size:
2884 754 531 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1619 3358 3412 4896 5061 50
Size: 1569 1739 54 1484 165 55
Fragments arranged by size:
1739 1569 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2978 2978
Size: 5066
BsrFI r'CCGG_y
Cuts at: 3064 3245 3454 3064
Size: 181 209 4676
Fragments arranged by size:
4676 209 181
BsrGI T'GTAC_A
Cuts at: 981 1875 981
Size: 894 4172
BssSI C'ACGA_G
Cuts at: 3337 3695 3337
Size: 358 4708
BstAPI GCAn_nnn'nTGC
Cuts at: 2443 2443
Size: 5066
BstBI TT'CG_AA
Cuts at: 3427 3427
Size: 5066
BstYI r'GATC_y
Cuts at: 1 1632 2091 2558 2581 2916 3162 4174 4260
Size: 1631 459 467 23 335 246 1012 86
Cuts at: 4260 4272 4618 1
Size: 12 346 449
Fragments arranged by size:
1631 1012 467 459 449 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2021 3016 3197 3360 2021
Size: 995 181 163 3727
Fragments arranged by size:
3727 995 181 163
BtsI GCAGTG_nn'
Cuts at: 2309 2309
Size: 5066
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1295 1330 1295
Size: 35 5031
DraI TTT'AAA
Cuts at: 2065 2065
Size: 5066
DrdI GACnn_nn'nnGTC
Cuts at: 2772 3630 2772
Size: 858 4208
EaeI y'GGCC_r
Cuts at: 2651 2825 3216 3243 4588 2651
Size: 174 391 27 1345 3129
Fragments arranged by size:
3129 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2651 2651
Size: 5066
EarI CTCTTCn'nnn_
Cuts at: 419 1409 3089 3299 419
Size: 990 1680 210 2186
Fragments arranged by size:
2186 1680 990 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3274 3584 3730 4876 3274
Size: 310 146 1146 3464
Fragments arranged by size:
3464 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2078 2078
Size: 5066
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 398 1319 1430 1915 2891 3323 4070 4338
Size: 171 921 111 485 976 432 747 268
Cuts at: 4338 227
Size: 955
Fragments arranged by size:
976 955 921 747 485 432 268 171
111
EcoO109I rG'GnC_Cy
Cuts at: 501 2205 501
Size: 1704 3362
EcoRI G'AATT_C
Cuts at: 2070 2070
Size: 5066
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1127 3085 1127
Size: 1958 3108
FspI TGC'GCA
Cuts at: 2847 2847
Size: 5066
HaeII r_GCGC'y
Cuts at: 2500 2748 3770 4749 2500
Size: 248 1022 979 2817
Fragments arranged by size:
2817 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 403 435 1144 1176 1234 1266 2029 2061 2908
Size: 32 709 32 58 32 763 32 847
Cuts at: 2908 2938 2940 2970 403
Size: 30 2 30 2499
Fragments arranged by size:
2499 847 763 709 58 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 319 1869 2105 319
Size: 1550 236 3280
Fragments arranged by size:
3280 1550 236
HindIII A'AGCT_T
Cuts at: 1780 1780
Size: 5066
KasI G'GCGC_C
Cuts at: 2496 2744 2496
Size: 248 4818
KpnI G_GTAC'C
Cuts at: 2086 2086
Size: 5066
MluI A'CGCG_T
Cuts at: 4499 4499
Size: 5066
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1688 3737 3921 1688
Size: 2049 184 2833
Fragments arranged by size:
2833 2049 184
MscI TGG'CCA
Cuts at: 2827 2827
Size: 5066
MslI CAynn'nnrTG
Cuts at: 953 1790 1896 3055 3182 953
Size: 837 106 1159 127 2837
Fragments arranged by size:
2837 1159 837 127 106
MspA1I CmG'CkG
Cuts at: 1995 2177 2491 2851 3864 4109 1995
Size: 182 314 360 1013 245 2952
Fragments arranged by size:
2952 1013 360 314 245 182
NaeI GCC'GGC
Cuts at: 3247 3247
Size: 5066
NarI GG'CG_CC
Cuts at: 2497 2745 2497
Size: 248 4818
NdeI CA'TA_TG
Cuts at: 1649 1649
Size: 5066
NgoMIV G'CCGG_C
Cuts at: 3245 3245
Size: 5066
NspI r_CATG'y
Cuts at: 611 1867 2119 2619 3526 611
Size: 1256 252 500 907 2151
Fragments arranged by size:
2151 1256 907 500 252
PciI A'CATG_T
Cuts at: 3522 3522
Size: 5066
PflMI CCAn_nnn'nTGG
Cuts at: 1295 1295
Size: 5066
PmeI GTTT'AAAC
Cuts at: 2065 2065
Size: 5066
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4205 4237 4205
Size: 32 5034
PpuMI rG'GwC_Cy
Cuts at: 501 501
Size: 5066
PshAI GACnn'nnGTC
Cuts at: 560 1591 560
Size: 1031 4035
PspOMI G'GGCC_C
Cuts at: 1765 1765
Size: 5066
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 965 997 965
Size: 32 5034
PstI C_TGCA'G
Cuts at: 842 2113 2798 842
Size: 1271 685 3110
Fragments arranged by size:
3110 1271 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5066
PvuII CAG'CTG
Cuts at: 2491 2851 2491
Size: 360 4706
RsrII CG'GwC_CG
Cuts at: 3261 3261
Size: 5066
SacI G_AGCT'C
Cuts at: 2080 2080
Size: 5066
SalI G'TCGA_C
Cuts at: 2103 2103
Size: 5066
SapI GCTCTTCn'nnn_
Cuts at: 3089 3299 3089
Size: 210 4856
SbfI CC_TGCA'GG
Cuts at: 2113 2113
Size: 5066
ScaI AGT'ACT
Cuts at: 370 1294 1420 4583 370
Size: 924 126 3163 853
Fragments arranged by size:
3163 924 853 126
SfcI C'TryA_G
Cuts at: 33 601 838 2109 2794 3787 3978 33
Size: 568 237 1271 685 993 191 1121
Fragments arranged by size:
1271 1121 993 685 568 237 191
SfoI GGC'GCC
Cuts at: 2498 2746 2498
Size: 248 4818
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5066
SmaI CCC'GGG
Cuts at: 436 2088 436
Size: 1652 3414
SmlI C'TyrA_G
Cuts at: 364 505 1999 2221 3136 3628 3890 364
Size: 141 1494 222 915 492 262 1540
Fragments arranged by size:
1540 1494 915 492 262 222 141
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5066
SphI G_CATG'C
Cuts at: 2119 2619 2119
Size: 500 4566
SspI AAT'ATT
Cuts at: 136 353 136
Size: 217 4849
StuI AGG'CCT
Cuts at: 286 286
Size: 5066
StyI C'CwwG_G
Cuts at: 578 2200 2554 578
Size: 1622 354 3090
Fragments arranged by size:
3090 1622 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2649 2782 3241 3450 2649
Size: 133 459 209 4265
Fragments arranged by size:
4265 459 209 133
TatI w'GTAC_w
Cuts at: 368 981 1292 1418 1875 4581 368
Size: 613 311 126 457 2706 853
Fragments arranged by size:
2706 853 613 457 311 126
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1667 1699 4583 4615 1667
Size: 32 2884 32 2118
Fragments arranged by size:
2884 2118 32 32
Tth111I GACn'n_nGTC
Cuts at: 2863 2863
Size: 5066
XbaI T'CTAG_A
Cuts at: 2097 2097
Size: 5066
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 202 2561 202
Size: 2359 2707
XmaI C'CCGG_G
Cuts at: 434 2086 434
Size: 1652 3414
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NspI PciI PflMI PmeI PpiI PpuMI PshAI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AlfI AscI AvrII BaeI BbvCI BcgI
BfrBI BmgBI BmtI BsgI BsiWI BssHII BstEII BstXI
BstZ17I Bsu36I BtgI ClaI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NcoI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PsiI PspXI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI XmnI
ZraI