Restriction map for
pF1KA0178
(Circular) (Six-base) MAPSORT of: pF1KA0178.seq Check: 6227 from: 1 to: 6812
pF1KA0178 6812 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 159 356 2120 3640 3850 159
Size: 197 1764 1520 210 3121
Fragments arranged by size:
3121 1764 1520 210 197
Acc65I G'GTAC_C
Cuts at: 731 3828 731
Size: 3097 3715
AclI AA'CG_TT
Cuts at: 6652 6652
Size: 6812
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 662 763 916 1891 2104 2611 2746 3097 3142
Size: 101 153 975 213 507 135 351 45
Cuts at: 3142 3437 4637 5069 5816 6084 662
Size: 295 1200 432 747 268 1390
Fragments arranged by size:
1390 1200 975 747 507 432 351 295
268 213 153 135 101 45
AfeI AGC'GCT
Cuts at: 2146 3304 2146
Size: 1158 5654
AflIII A'CryG_T
Cuts at: 2615 4164 5268 6245 2615
Size: 1549 1104 977 3182
Fragments arranged by size:
3182 1549 1104 977
AgeI A'CCGG_T
Cuts at: 728 1235 5200 728
Size: 507 3965 2340
Fragments arranged by size:
3965 2340 507
AhdI GACnn_n'nnGTC
Cuts at: 3489 3489
Size: 6812
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 943 943
Size: 6812
AlwNI CAG_nnn'CTG
Cuts at: 741 1284 2739 3135 4117 5684 741
Size: 543 1455 396 982 1567 1869
Fragments arranged by size:
1869 1567 1455 982 543 396
ApaI G_GGCC'C
Cuts at: 3406 3406
Size: 6812
ApaLI G'TGCA_C
Cuts at: 5582 5582
Size: 6812
ApoI r'AATT_y
Cuts at: 1291 3011 3816 6165 6421 1291
Size: 1720 805 2349 256 1682
Fragments arranged by size:
2349 1720 1682 805 256
AseI AT'TA_AT
Cuts at: 20 2351 20
Size: 2331 4481
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6812
AvaI C'yCGr_G
Cuts at: 2800 3832 2800
Size: 1032 5780
BamHI G'GATC_C
Cuts at: 3837 3837
Size: 6812
BanI G'GyrC_C
Cuts at: 731 2806 2941 3828 4242 4490 4525 731
Size: 2075 135 887 414 248 35 3018
Fragments arranged by size:
3018 2075 887 414 248 135 35
BanII G_rGCy'C
Cuts at: 312 765 3406 3425 3826 4856 312
Size: 453 2641 19 401 1030 2268
Fragments arranged by size:
2641 2268 1030 453 401 19
BbeI G_GCGC'C
Cuts at: 2810 4246 4494 2810
Size: 1436 248 5128
Fragments arranged by size:
5128 1436 248
BbsI GAAGACnn'nnnn_
Cuts at: 1765 2554 3511 1765
Size: 789 957 5066
Fragments arranged by size:
5066 957 789
BbvCI CC'TCA_GC
Cuts at: 374 3092 374
Size: 2718 4094
BciVI GTATCCnnnnn_n'
Cuts at: 4710 5471 6812 4710
Size: 761 1341 4710
Fragments arranged by size:
4710 1341 761
BclI T'GATC_A
Cuts at: 3740 4332 3740
Size: 592 6220
BglI GCCn_nnn'nGGC
Cuts at: 337 3277 337
Size: 2940 3872
BglII A'GATC_T
Cuts at: 3356 4327 6364 3356
Size: 971 2037 3804
Fragments arranged by size:
3804 2037 971
BlpI GC'TnA_GC
Cuts at: 2711 3924 2711
Size: 1213 5599
Bme1580I G_kGCm'C
Cuts at: 3390 3406 4437 4530 5586 3390
Size: 16 1031 93 1056 4616
Fragments arranged by size:
4616 1056 1031 93 16
BmrI ACTGGGnnnn_n'
Cuts at: 4024 4105 4440 6584 4024
Size: 81 335 2144 4252
Fragments arranged by size:
4252 2144 335 81
BmtI G_CTAG'C
Cuts at: 2332 2332
Size: 6812
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 581 987 1038 2991 3055 5179 581
Size: 406 51 1953 64 2124 2214
Fragments arranged by size:
2214 2124 1953 406 64 51
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1135 1306 1441 1855 2338 1135
Size: 171 135 414 483 5609
Fragments arranged by size:
5609 483 414 171 135
Bpu10I CC'TnA_GC
Cuts at: 374 764 3092 3722 374
Size: 390 2328 630 3464
Fragments arranged by size:
3464 2328 630 390
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2227 3658 3988 4867 5359 5657 2227
Size: 1431 330 879 492 298 3382
Fragments arranged by size:
3382 1431 879 492 330 298
BsaI GGTCTCn'nnnn_
Cuts at: 1295 1804 2695 2848 3220 6516 1295
Size: 509 891 153 372 3296 1591
Fragments arranged by size:
3296 1591 891 509 372 153
BsaAI yAC'GTr
Cuts at: 4165 4165
Size: 6812
BsaBI GATnn'nnATC
Cuts at: 6 4326 6363 6
Size: 4320 2037 455
Fragments arranged by size:
4320 2037 455
BsaHI Gr'CG_yC
Cuts at: 2807 4243 4491 5193 6689 2807
Size: 1436 248 702 1496 2930
Fragments arranged by size:
2930 1496 1436 702 248
BsaWI w'CCGG_w
Cuts at: 728 1235 4001 4522 5200 5474 5621 6032 728
Size: 507 2766 521 678 274 147 411 1508
Fragments arranged by size:
2766 1508 678 521 507 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 299 329 1114 1144 299
Size: 30 785 30 5967
Fragments arranged by size:
5967 785 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 379 1225 1504 2170 2797 3694 379
Size: 846 279 666 627 897 3497
Fragments arranged by size:
3497 897 846 666 627 279
BseYI C'CCAG_C
Cuts at: 915 2102 4237 4995 5572 6068 915
Size: 1187 2135 758 577 496 1659
Fragments arranged by size:
2135 1659 1187 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2230 2230
Size: 6812
BsiEI CG_ry'CG
Cuts at: 104 1578 1659 4354 4400 5608 104
Size: 1474 81 2695 46 1208 1308
Fragments arranged by size:
2695 1474 1308 1208 81 46
BsiHKAI G_wGCw'C
Cuts at: 312 3826 4604 4794 5586 312
Size: 3514 778 190 792 1538
Fragments arranged by size:
3514 1538 792 778 190
BsmI GAATG_Cn'
Cuts at: 482 1074 2561 3280 3394 482
Size: 592 1487 719 114 3900
Fragments arranged by size:
3900 1487 719 592 114
Bsp1286I G_dGCh'C
Cuts at: 312 765 3390 3406 3425 3826 4437 4530 4604
Size: 453 2625 16 19 401 611 93 74
Cuts at: 4604 4794 4856 5586 312
Size: 190 62 730 1538
Fragments arranged by size:
2625 1538 730 611 453 401 190 93
74 62 19 16
BspEI T'CCGG_A
Cuts at: 6032 6032
Size: 6812
BspHI T'CATG_A
Cuts at: 2772 5988 2772
Size: 3216 3596
BspMI ACCTGCnnnn'nnnn_
Cuts at: 281 3795 3862 4076 4378 4759 281
Size: 3514 67 214 302 381 2334
Fragments arranged by size:
3514 2334 381 302 214 67
BsrBI CCG'CTC
Cuts at: 50 5104 5158 6642 6807 50
Size: 5054 54 1484 165 55
Fragments arranged by size:
5054 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1956 1988 4724 1956
Size: 32 2736 4044
Fragments arranged by size:
4044 2736 32
BsrFI r'CCGG_y
Cuts at: 728 1235 4810 4991 5200 728
Size: 507 3575 181 209 2340
Fragments arranged by size:
3575 2340 507 209 181
BssSI C'ACGA_G
Cuts at: 3029 5083 5441 3029
Size: 2054 358 4400
Fragments arranged by size:
4400 2054 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1380 3153 4189 1380
Size: 1773 1036 4003
Fragments arranged by size:
4003 1773 1036
BstBI TT'CG_AA
Cuts at: 3284 5173 3284
Size: 1889 4923
BstXI CCAn_nnnn'nTGG
Cuts at: 1719 1746 1719
Size: 27 6785
BstYI r'GATC_y
Cuts at: 1 1329 3072 3356 3837 4304 4327 4662 4908
Size: 1328 1743 284 481 467 23 335 246
Cuts at: 4908 5920 6006 6018 6364 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1743 1328 1012 481 467 449 346 335
284 246 86 23 12
BtgI C'CryG_G
Cuts at: 107 303 2223 2705 2945 3188 107
Size: 196 1920 482 240 243 3731
Fragments arranged by size:
3731 1920 482 243 240 196
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 652 4762 4943 5106 652
Size: 4110 181 163 2358
Fragments arranged by size:
4110 2358 181 163
BtsI GCAGTG_nn'
Cuts at: 2883 3121 3390 4055 2883
Size: 238 269 665 5640
Fragments arranged by size:
5640 665 269 238
DraI TTT'AAA
Cuts at: 3811 3811
Size: 6812
DrdI GACnn_nn'nnGTC
Cuts at: 4518 5376 4518
Size: 858 5954
EaeI y'GGCC_r
Cuts at: 1502 1656 2741 4397 4571 4962 4989 6334 1502
Size: 154 1085 1656 174 391 27 1345 1980
Fragments arranged by size:
1980 1656 1345 1085 391 174 154 27
EagI C'GGCC_G
Cuts at: 1656 4397 1656
Size: 2741 4071
EarI CTCTTCn'nnn_
Cuts at: 569 638 713 1169 1388 1451 2435 2960 3092
Size: 69 75 456 219 63 984 525 132
Cuts at: 3092 3413 4835 5045 569
Size: 321 1422 210 2336
Fragments arranged by size:
2336 1422 984 525 456 321 219 210
132 75 69 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5020 5330 5476 6622 5020
Size: 310 146 1146 5210
Fragments arranged by size:
5210 1146 310 146
EcoICRI GAG'CTC
Cuts at: 310 3824 310
Size: 3514 3298
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 662 763 916 1135 1306 1441 1855 1891 2104
Size: 101 153 219 171 135 414 36 213
Cuts at: 2104 2338 2611 2746 3097 3142 3437 4637 5069
Size: 234 273 135 351 45 295 1200 432
Cuts at: 5069 5816 6084 662
Size: 747 268 1390
Fragments arranged by size:
1390 1200 747 432 414 351 295 273
268 234 219 213 171 153 135 135
101 45 36
EcoNI CCTnn'n_nnAGG
Cuts at: 1276 3725 1276
Size: 2449 4363
EcoO109I rG'GnC_Cy
Cuts at: 294 1929 3036 3366 3403 3951 294
Size: 1635 1107 330 37 548 3155
Fragments arranged by size:
3155 1635 1107 548 330 37
EcoRI G'AATT_C
Cuts at: 3816 3816
Size: 6812
EcoRV GAT'ATC
Cuts at: 2358 2358
Size: 6812
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2163 2571 3321 4831 2163
Size: 408 750 1510 4144
Fragments arranged by size:
4144 1510 750 408
FspI TGC'GCA
Cuts at: 2467 4593 2467
Size: 2126 4686
HaeII r_GCGC'y
Cuts at: 741 1635 2013 2103 2148 2810 3306 4246 4494
Size: 894 378 90 45 662 496 940 248
Cuts at: 4494 5516 6495 741
Size: 1022 979 1058
Fragments arranged by size:
1058 1022 979 940 894 662 496 378
248 90 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 152 184 1114 1146 1170 1202 2268 2300 2694
Size: 32 930 32 24 32 1066 32 394
Cuts at: 2694 2726 3475 3507 4654 4684 4686 4716 152
Size: 32 749 32 1147 30 2 30 2248
Fragments arranged by size:
2248 1147 1066 930 749 394 32 32
32 32 32 32 30 30 24 2
HincII GTy'rAC
Cuts at: 160 3641 3851 160
Size: 3481 210 3121
Fragments arranged by size:
3481 3121 210
HindIII A'AGCT_T
Cuts at: 787 2860 787
Size: 2073 4739
KasI G'GCGC_C
Cuts at: 2806 4242 4490 2806
Size: 1436 248 5128
Fragments arranged by size:
5128 1436 248
KpnI G_GTAC'C
Cuts at: 735 3832 735
Size: 3097 3715
MluI A'CGCG_T
Cuts at: 6245 6245
Size: 6812
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 468 1187 2549 2732 5483 5667 468
Size: 719 1362 183 2751 184 1613
Fragments arranged by size:
2751 1613 1362 719 184 183
MscI TGG'CCA
Cuts at: 1504 2743 4573 1504
Size: 1239 1830 3743
Fragments arranged by size:
3743 1830 1239
MslI CAynn'nnrTG
Cuts at: 1744 4801 4928 1744
Size: 3057 127 3628
Fragments arranged by size:
3628 3057 127
MspA1I CmG'CkG
Cuts at: 328 774 3923 4237 4597 5610 5855 328
Size: 446 3149 314 360 1013 245 1285
Fragments arranged by size:
3149 1285 1013 446 360 314 245
NaeI GCC'GGC
Cuts at: 4993 4993
Size: 6812
NarI GG'CG_CC
Cuts at: 2807 4243 4491 2807
Size: 1436 248 5128
Fragments arranged by size:
5128 1436 248
NcoI C'CATG_G
Cuts at: 107 303 2223 2705 2945 3188 107
Size: 196 1920 482 240 243 3731
Fragments arranged by size:
3731 1920 482 243 240 196
NgoMIV G'CCGG_C
Cuts at: 4991 4991
Size: 6812
NheI G'CTAG_C
Cuts at: 2328 2328
Size: 6812
NspI r_CATG'y
Cuts at: 2619 3865 4365 5272 2619
Size: 1246 500 907 4159
Fragments arranged by size:
4159 1246 907 500
PasI CC'CwG_GG
Cuts at: 2979 2979
Size: 6812
PciI A'CATG_T
Cuts at: 2615 5268 2615
Size: 2653 4159
PflMI CCAn_nnn'nTGG
Cuts at: 1284 1548 1284
Size: 264 6548
PfoI T'CCnGG_A
Cuts at: 1862 2474 3221 3569 1862
Size: 612 747 348 5105
Fragments arranged by size:
5105 747 612 348
PmeI GTTT'AAAC
Cuts at: 3811 3811
Size: 6812
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5951 5983 5951
Size: 32 6780
PpuMI rG'GwC_Cy
Cuts at: 294 294
Size: 6812
PspOMI G'GGCC_C
Cuts at: 3402 3402
Size: 6812
PstI C_TGCA'G
Cuts at: 779 1067 1937 2234 2303 3128 3859 4544 779
Size: 288 870 297 69 825 731 685 3047
Fragments arranged by size:
3047 870 825 731 685 297 288 69
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6812
PvuII CAG'CTG
Cuts at: 328 774 4237 4597 328
Size: 446 3463 360 2543
Fragments arranged by size:
3463 2543 446 360
RsrII CG'GwC_CG
Cuts at: 5007 5007
Size: 6812
SacI G_AGCT'C
Cuts at: 312 3826 312
Size: 3514 3298
SalI G'TCGA_C
Cuts at: 158 3639 3849 158
Size: 3481 210 3121
Fragments arranged by size:
3481 3121 210
SapI GCTCTTCn'nnn_
Cuts at: 1451 3413 4835 5045 1451
Size: 1962 1422 210 3218
Fragments arranged by size:
3218 1962 1422 210
SbfI CC_TGCA'GG
Cuts at: 3859 3859
Size: 6812
ScaI AGT'ACT
Cuts at: 2248 6329 2248
Size: 4081 2731
SexAI A'CCwGG_T
Cuts at: 1541 2417 1541
Size: 876 5936
SfcI C'TryA_G
Cuts at: 33 775 1063 1847 1933 2230 2299 2896 3124
Size: 742 288 784 86 297 69 597 228
Cuts at: 3124 3855 4540 5533 5724 33
Size: 731 685 993 191 1121
Fragments arranged by size:
1121 993 784 742 731 685 597 297
288 228 191 86 69
SfoI GGC'GCC
Cuts at: 2808 4244 4492 2808
Size: 1436 248 5128
Fragments arranged by size:
5128 1436 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6812
SmaI CCC'GGG
Cuts at: 3834 3834
Size: 6812
SmlI C'TyrA_G
Cuts at: 2242 3673 3967 4882 5374 5636 2242
Size: 1431 294 915 492 262 3418
Fragments arranged by size:
3418 1431 915 492 294 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6812
SphI G_CATG'C
Cuts at: 3865 4365 3865
Size: 500 6312
SspI AAT'ATT
Cuts at: 681 681
Size: 6812
StuI AGG'CCT
Cuts at: 2902 2902
Size: 6812
StyI C'CwwG_G
Cuts at: 107 303 1010 1712 2090 2223 2705 2945 3188
Size: 196 707 702 378 133 482 240 243
Cuts at: 3188 3590 3692 3946 4300 107
Size: 402 102 254 354 2619
Fragments arranged by size:
2619 707 702 482 402 378 354 254
243 240 196 133 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 4395 4528 4987 5196 4395
Size: 133 459 209 6011
Fragments arranged by size:
6011 459 209 133
TatI w'GTAC_w
Cuts at: 419 455 998 2246 2610 6327 419
Size: 36 543 1248 364 3717 904
Fragments arranged by size:
3717 1248 904 543 364 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 299 331 6329 6361 299
Size: 32 5998 32 750
Fragments arranged by size:
5998 750 32 32
Tth111I GACn'n_nGTC
Cuts at: 4609 4609
Size: 6812
XbaI T'CTAG_A
Cuts at: 3843 3843
Size: 6812
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2526 3195 4307 2526
Size: 669 1112 5031
Fragments arranged by size:
5031 1112 669
XmaI C'CCGG_G
Cuts at: 3832 3832
Size: 6812
XmnI GAAnn'nnTTC
Cuts at: 3469 3469
Size: 6812
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AleI AloI AscI AvrII BaeI
BcgI BfrBI BmgBI BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII FseI FspAI HpaI
MfeI NdeI NotI NruI NsiI PacI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SwaI XhoI ZraI