Restriction map for
pF1KA0182
(Circular) (Six-base) MAPSORT of: pF1KA0182.seq Check: 1180 from: 1 to: 6545
pF1KA0182 6545 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 422 791 1440 3312 422
Size: 369 649 1872 3655
Fragments arranged by size:
3655 1872 649 369
AccI GT'mk_AC
Cuts at: 2425 3342 3583 2425
Size: 917 241 5387
Fragments arranged by size:
5387 917 241
Acc65I G'GTAC_C
Cuts at: 3561 3561
Size: 6545
AclI AA'CG_TT
Cuts at: 6385 6385
Size: 6545
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 666 1228 2026 3544 4370 4802 5549 5817 666
Size: 562 798 1518 826 432 747 268 1394
Fragments arranged by size:
1518 1394 826 798 747 562 432 268
AfeI AGC'GCT
Cuts at: 839 2517 839
Size: 1678 4867
AflIII A'CryG_T
Cuts at: 2249 3062 3897 5001 5978 2249
Size: 813 835 1104 977 2816
Fragments arranged by size:
2816 1104 977 835 813
AgeI A'CCGG_T
Cuts at: 4933 4933
Size: 6545
AhdI GACnn_n'nnGTC
Cuts at: 1422 1422
Size: 6545
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1636 1668 1636
Size: 32 6513
AlwNI CAG_nnn'CTG
Cuts at: 1455 1467 2766 3850 5417 1455
Size: 12 1299 1084 1567 2583
Fragments arranged by size:
2583 1567 1299 1084 12
ApaI G_GGCC'C
Cuts at: 208 1068 1901 1907 2046 2208 3163 208
Size: 860 833 6 139 162 955 3590
Fragments arranged by size:
3590 955 860 833 162 139 6
ApaLI G'TGCA_C
Cuts at: 502 5315 502
Size: 4813 1732
ApoI r'AATT_y
Cuts at: 3549 5898 6154 3549
Size: 2349 256 3940
Fragments arranged by size:
3940 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6545
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6545
AvaI C'yCGr_G
Cuts at: 811 1067 1193 1894 1900 1921 3565 811
Size: 256 126 701 6 21 1644 3791
Fragments arranged by size:
3791 1644 701 256 126 21 6
AvrII C'CTAG_G
Cuts at: 3511 3511
Size: 6545
BamHI G'GATC_C
Cuts at: 3570 3570
Size: 6545
BanI G'GyrC_C
Cuts at: 513 1227 1260 1281 1488 3561 3975 4223 4258
Size: 714 33 21 207 2073 414 248 35
Cuts at: 4258 513
Size: 2800
Fragments arranged by size:
2800 2073 714 414 248 207 35 33
21
BanII G_rGCy'C
Cuts at: 208 368 818 1068 1106 1730 1901 1907 1922
Size: 160 450 250 38 624 171 6 15
Cuts at: 1922 1994 2046 2208 2939 3143 3163 3559 4589
Size: 72 52 162 731 204 20 396 1030
Cuts at: 4589 208
Size: 2164
Fragments arranged by size:
2164 1030 731 624 450 396 250 204
171 162 160 72 52 38 20 15
6
BbeI G_GCGC'C
Cuts at: 1231 3979 4227 1231
Size: 2748 248 3549
Fragments arranged by size:
3549 2748 248
BbsI GAAGACnn'nnnn_
Cuts at: 1764 1764
Size: 6545
BbvCI CC'TCA_GC
Cuts at: 865 3370 865
Size: 2505 4040
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 765 799 765
Size: 34 6511
BciVI GTATCCnnnnn_n'
Cuts at: 4443 5204 6545 4443
Size: 761 1341 4443
Fragments arranged by size:
4443 1341 761
BclI T'GATC_A
Cuts at: 4065 4065
Size: 6545
BglI GCCn_nnn'nGGC
Cuts at: 1093 2025 1093
Size: 932 5613
BglII A'GATC_T
Cuts at: 4060 6097 4060
Size: 2037 4508
BlpI GC'TnA_GC
Cuts at: 418 1916 1993 2815 3657 418
Size: 1498 77 822 842 3306
Fragments arranged by size:
3306 1498 842 822 77
Bme1580I G_kGCm'C
Cuts at: 200 208 242 506 518 1068 1265 1286 1901
Size: 8 34 264 12 550 197 21 615
Cuts at: 1901 1907 2046 2208 3163 4170 4263 5319 200
Size: 6 139 162 955 1007 93 1056 1426
Fragments arranged by size:
1426 1056 1007 955 615 550 264 197
162 139 93 34 21 12 8 6
BmgBI CAC'GTC
Cuts at: 2252 3065 2252
Size: 813 5732
BmrI ACTGGGnnnn_n'
Cuts at: 515 2026 3757 3838 4173 6317 515
Size: 1511 1731 81 335 2144 743
Fragments arranged by size:
2144 1731 1511 743 335 81
BmtI G_CTAG'C
Cuts at: 2788 2788
Size: 6545
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3408 4912 3408
Size: 1504 5041
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 309 315 475 1063 1120 1477 1894 2134 2849
Size: 6 160 588 57 357 417 240 715
Cuts at: 2849 3084 3334 3370 3439 309
Size: 235 250 36 69 3415
Fragments arranged by size:
3415 715 588 417 357 250 240 235
160 69 57 36 6
Bpu10I CC'TnA_GC
Cuts at: 865 898 3086 3370 865
Size: 33 2188 284 4040
Fragments arranged by size:
4040 2188 284 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3721 4600 5092 5390 3721
Size: 879 492 298 4876
Fragments arranged by size:
4876 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3441 6249 3441
Size: 2808 3737
BsaAI yAC'GTr
Cuts at: 510 3898 510
Size: 3388 3157
BsaBI GATnn'nnATC
Cuts at: 6 4059 6096 6
Size: 4053 2037 455
Fragments arranged by size:
4053 2037 455
BsaHI Gr'CG_yC
Cuts at: 1228 2401 3976 4224 4926 6422 1228
Size: 1173 1575 248 702 1496 1351
Fragments arranged by size:
1575 1496 1351 1173 702 248
BsaWI w'CCGG_w
Cuts at: 1257 3734 4255 4933 5207 5354 5765 1257
Size: 2477 521 678 274 147 411 2037
Fragments arranged by size:
2477 2037 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 154 184 252 282 321 351 473 503 841
Size: 30 68 30 39 30 122 30 338
Cuts at: 841 871 1360 1390 2134 2164 2598 2628 154
Size: 30 489 30 744 30 434 30 4071
Fragments arranged by size:
4071 744 489 434 338 122 68 39
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 312 907 1171 1381 1384 1807 2131 2206 2209
Size: 595 264 210 3 423 324 75 3
Cuts at: 2209 3208 3211 3220 3247 3429 312
Size: 999 3 9 27 182 3428
Fragments arranged by size:
3428 999 595 423 324 264 210 182
75 27 9 3 3 3
BseYI C'CCAG_C
Cuts at: 241 448 643 1105 1285 1613 1729 3119 3970
Size: 207 195 462 180 328 116 1390 851
Cuts at: 3970 4728 5305 5801 241
Size: 758 577 496 985
Fragments arranged by size:
1390 985 851 758 577 496 462 328
207 195 180 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 748 769 826 1610 1687 2858 481
Size: 267 21 57 784 77 1171 4168
Fragments arranged by size:
4168 1171 784 267 77 57 21
BsiEI CG_ry'CG
Cuts at: 104 4087 4133 5341 104
Size: 3983 46 1208 1308
Fragments arranged by size:
3983 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 506 818 1508 1522 1880 1922 1994 3559 4337
Size: 312 690 14 358 42 72 1565 778
Cuts at: 4337 4527 5319 506
Size: 190 792 1732
Fragments arranged by size:
1732 1565 792 778 690 358 312 190
72 42 14
BsmBI CGTCTCn'nnnn_
Cuts at: 1669 2640 1669
Size: 971 5574
Bsp1286I G_dGCh'C
Cuts at: 200 208 242 368 506 518 818 1068 1106
Size: 8 34 126 138 12 300 250 38
Cuts at: 1106 1265 1286 1508 1522 1730 1880 1901 1907
Size: 159 21 222 14 208 150 21 6
Cuts at: 1907 1922 1994 2046 2208 2939 3143 3163 3559
Size: 15 72 52 162 731 204 20 396
Cuts at: 3559 4170 4263 4337 4527 4589 5319 200
Size: 611 93 74 190 62 730 1426
Fragments arranged by size:
1426 731 730 611 396 300 250 222
208 204 190 162 159 150 138 126
93 74 72 62 52 38 34 21
21 20 15 14 12 8 6
BspEI T'CCGG_A
Cuts at: 5765 5765
Size: 6545
BspHI T'CATG_A
Cuts at: 5721 5721
Size: 6545
BspMI ACCTGCnnnn'nnnn_
Cuts at: 422 791 1440 3312 3595 3809 4111 4492 422
Size: 369 649 1872 283 214 302 381 2475
Fragments arranged by size:
2475 1872 649 381 369 302 283 214
BsrBI CCG'CTC
Cuts at: 50 372 957 1161 1767 3318 3450 4837 4891
Size: 322 585 204 606 1551 132 1387 54
Cuts at: 4891 6375 6540 50
Size: 1484 165 55
Fragments arranged by size:
1551 1484 1387 606 585 322 204 165
132 55 54
BsrDI GCAATG_nn'
Cuts at: 3506 4457 3506
Size: 951 5594
BsrFI r'CCGG_y
Cuts at: 308 319 1418 1478 1491 2024 2685 4543 4724
Size: 11 1099 60 13 533 661 1858 181
Cuts at: 4724 4933 308
Size: 209 1920
Fragments arranged by size:
1920 1858 1099 661 533 209 181 60
13 11
BssSI C'ACGA_G
Cuts at: 1546 1856 2971 4816 5174 1546
Size: 310 1115 1845 358 2917
Fragments arranged by size:
2917 1845 1115 358 310
BstAPI GCAn_nnn'nTGC
Cuts at: 1137 1260 1452 3922 1137
Size: 123 192 2470 3760
Fragments arranged by size:
3760 2470 192 123
BstBI TT'CG_AA
Cuts at: 2468 4906 2468
Size: 2438 4107
BstEII G'GTnAC_C
Cuts at: 258 258
Size: 6545
BstXI CCAn_nnnn'nTGG
Cuts at: 651 1098 3127 651
Size: 447 2029 4069
Fragments arranged by size:
4069 2029 447
BstYI r'GATC_y
Cuts at: 1 3570 4037 4060 4395 4641 5653 5739 5751
Size: 3569 467 23 335 246 1012 86 12
Cuts at: 5751 6097 1
Size: 346 449
Fragments arranged by size:
3569 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 377 715 377
Size: 338 6207
BtgI C'CryG_G
Cuts at: 200 254 1118 1302 1631 1801 2038 2726 2929
Size: 54 864 184 329 170 237 688 203
Cuts at: 2929 200
Size: 3816
Fragments arranged by size:
3816 864 688 329 237 203 184 170
54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 249 790 2201 4495 4676 4839 249
Size: 541 1411 2294 181 163 1955
Fragments arranged by size:
2294 1955 1411 541 181 163
BtsI GCAGTG_nn'
Cuts at: 342 592 620 760 1335 2597 3146 3788 342
Size: 250 28 140 575 1262 549 642 3099
Fragments arranged by size:
3099 1262 642 575 549 250 140 28
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2197 2232 2197
Size: 35 6510
DraI TTT'AAA
Cuts at: 3544 3544
Size: 6545
DraIII CAC_nnn'GTG
Cuts at: 237 510 513 1281 3402 237
Size: 273 3 768 2121 3380
Fragments arranged by size:
3380 2121 768 273 3
DrdI GACnn_nn'nnGTC
Cuts at: 1541 3067 4251 5109 1541
Size: 1526 1184 858 2977
Fragments arranged by size:
2977 1526 1184 858
EaeI y'GGCC_r
Cuts at: 155 617 1085 1121 1143 1557 1976 2222 2594
Size: 462 468 36 22 414 419 246 372
Cuts at: 2594 4130 4304 4695 4722 6067 155
Size: 1536 174 391 27 1345 633
Fragments arranged by size:
1536 1345 633 468 462 419 414 391
372 246 174 36 27 22
EagI C'GGCC_G
Cuts at: 4130 4130
Size: 6545
EarI CTCTTCn'nnn_
Cuts at: 2518 2914 2945 3197 3296 4568 4778 2518
Size: 396 31 252 99 1272 210 4285
Fragments arranged by size:
4285 1272 396 252 210 99 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1510 1835 2358 3421 4753 5063 5209 6355 1510
Size: 325 523 1063 1332 310 146 1146 1700
Fragments arranged by size:
1700 1332 1146 1063 523 325 310 146
EcoICRI GAG'CTC
Cuts at: 816 1920 1992 3557 816
Size: 1104 72 1565 3804
Fragments arranged by size:
3804 1565 1104 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 309 315 475 666 1063 1120 1228 1477 1894
Size: 6 160 191 397 57 108 249 417
Cuts at: 1894 2026 2134 2849 3084 3334 3370 3439 3544
Size: 132 108 715 235 250 36 69 105
Cuts at: 3544 4370 4802 5549 5817 309
Size: 826 432 747 268 1037
Fragments arranged by size:
1037 826 747 715 432 417 397 268
250 249 235 191 160 132 108 108
105 69 57 36 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1969 1969
Size: 6545
EcoO109I rG'GnC_Cy
Cuts at: 116 205 719 1095 1904 2043 2204 2823 3000
Size: 89 514 376 809 139 161 619 177
Cuts at: 3000 3159 3160 3684 116
Size: 159 1 524 2977
Fragments arranged by size:
2977 809 619 524 514 376 177 161
159 139 89 1
EcoRI G'AATT_C
Cuts at: 3549 3549
Size: 6545
EcoRV GAT'ATC
Cuts at: 3532 3532
Size: 6545
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2560 4564 2560
Size: 2004 4541
FseI GG_CCGG'CC
Cuts at: 1482 1482
Size: 6545
FspI TGC'GCA
Cuts at: 4326 4326
Size: 6545
HaeII r_GCGC'y
Cuts at: 841 1050 1231 2088 2422 2519 2873 3979 4227
Size: 209 181 857 334 97 354 1106 248
Cuts at: 4227 5249 6228 841
Size: 1022 979 1158
Fragments arranged by size:
1158 1106 1022 979 857 354 334 248
209 181 97
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 252 284 307 339 386 418 841 873 1408
Size: 32 23 32 47 32 423 32 535
Cuts at: 1408 1440 2239 2271 2598 2610 2630 2642 3079
Size: 32 799 32 327 12 20 12 437
Cuts at: 3079 3111 3172 3204 4387 4417 4419 4449 252
Size: 32 61 32 1183 30 2 30 2348
Fragments arranged by size:
2348 1183 799 535 437 423 327 61
47 32 32 32 32 32 32 32
32 30 30 23 20 12 12 2
HincII GTy'rAC
Cuts at: 3060 3584 3060
Size: 524 6021
HindIII A'AGCT_T
Cuts at: 549 3281 549
Size: 2732 3813
KasI G'GCGC_C
Cuts at: 1227 3975 4223 1227
Size: 2748 248 3549
Fragments arranged by size:
3549 2748 248
KpnI G_GTAC'C
Cuts at: 3565 3565
Size: 6545
MluI A'CGCG_T
Cuts at: 5978 5978
Size: 6545
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 295 1266 1322 3158 5216 5400 295
Size: 971 56 1836 2058 184 1440
Fragments arranged by size:
2058 1836 1440 971 184 56
MscI TGG'CCA
Cuts at: 1145 4306 1145
Size: 3161 3384
MslI CAynn'nnrTG
Cuts at: 2890 4534 4661 2890
Size: 1644 127 4774
Fragments arranged by size:
4774 1644 127
MspA1I CmG'CkG
Cuts at: 495 1053 1062 1182 1392 1752 1956 1997 2728
Size: 558 9 120 210 360 204 41 731
Cuts at: 2728 2798 2898 3656 3970 4330 5343 5588 495
Size: 70 100 758 314 360 1013 245 1452
Fragments arranged by size:
1452 1013 758 731 558 360 360 314
245 210 204 120 100 70 41 9
NaeI GCC'GGC
Cuts at: 310 321 1480 1493 4726 310
Size: 11 1159 13 3233 2129
Fragments arranged by size:
3233 2129 1159 13 11
NarI GG'CG_CC
Cuts at: 1228 3976 4224 1228
Size: 2748 248 3549
Fragments arranged by size:
3549 2748 248
NcoI C'CATG_G
Cuts at: 200 1302 200
Size: 1102 5443
NgoMIV G'CCGG_C
Cuts at: 308 319 1478 1491 4724 308
Size: 11 1159 13 3233 2129
Fragments arranged by size:
3233 2129 1159 13 11
NheI G'CTAG_C
Cuts at: 2784 2784
Size: 6545
NspI r_CATG'y
Cuts at: 588 858 2853 3598 4098 5005 588
Size: 270 1995 745 500 907 2128
Fragments arranged by size:
2128 1995 907 745 500 270
PasI CC'CwG_GG
Cuts at: 223 3536 223
Size: 3313 3232
PciI A'CATG_T
Cuts at: 5001 5001
Size: 6545
PflMI CCAn_nnn'nTGG
Cuts at: 3177 3402 3177
Size: 225 6320
PfoI T'CCnGG_A
Cuts at: 325 2777 325
Size: 2452 4093
PmeI GTTT'AAAC
Cuts at: 3544 3544
Size: 6545
PmlI CAC'GTG
Cuts at: 510 510
Size: 6545
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5684 5716 5684
Size: 32 6513
PpuMI rG'GwC_Cy
Cuts at: 116 719 116
Size: 603 5942
PspOMI G'GGCC_C
Cuts at: 204 1064 1897 1903 2042 2204 3159 204
Size: 860 833 6 139 162 955 3590
Fragments arranged by size:
3590 955 860 833 162 139 6
PstI C_TGCA'G
Cuts at: 630 884 1226 2081 2987 3008 3042 3095 3592
Size: 254 342 855 906 21 34 53 497
Cuts at: 3592 4277 630
Size: 685 2898
Fragments arranged by size:
2898 906 855 685 497 342 254 53
34 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6545
PvuII CAG'CTG
Cuts at: 1182 3970 4330 1182
Size: 2788 360 3397
Fragments arranged by size:
3397 2788 360
RsrII CG'GwC_CG
Cuts at: 4740 4740
Size: 6545
SacI G_AGCT'C
Cuts at: 818 1922 1994 3559 818
Size: 1104 72 1565 3804
Fragments arranged by size:
3804 1565 1104 72
SacII CC_GC'GG
Cuts at: 2729 2729
Size: 6545
SalI G'TCGA_C
Cuts at: 3582 3582
Size: 6545
SapI GCTCTTCn'nnn_
Cuts at: 2914 3296 4568 4778 2914
Size: 382 1272 210 4681
Fragments arranged by size:
4681 1272 382 210
SbfI CC_TGCA'GG
Cuts at: 1226 3592 1226
Size: 2366 4179
ScaI AGT'ACT
Cuts at: 6062 6062
Size: 6545
SfcI C'TryA_G
Cuts at: 33 626 880 1222 2077 2433 2983 3004 3038
Size: 593 254 342 855 356 550 21 34
Cuts at: 3038 3091 3363 3588 4273 5266 5457 33
Size: 53 272 225 685 993 191 1121
Fragments arranged by size:
1121 993 855 685 593 550 356 342
272 254 225 191 53 34 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 1093 1093
Size: 6545
SfoI GGC'GCC
Cuts at: 1229 3977 4225 1229
Size: 2748 248 3549
Fragments arranged by size:
3549 2748 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6545
SmaI CCC'GGG
Cuts at: 1069 1896 1902 3567 1069
Size: 827 6 1665 4047
Fragments arranged by size:
4047 1665 827 6
SmlI C'TyrA_G
Cuts at: 1921 3700 4615 5107 5369 1921
Size: 1779 915 492 262 3097
Fragments arranged by size:
3097 1779 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6545
SphI G_CATG'C
Cuts at: 2853 3598 4098 2853
Size: 745 500 5300
Fragments arranged by size:
5300 745 500
SrfI GCCC'GGGC
Cuts at: 1896 1902 1896
Size: 6 6539
StyI C'CwwG_G
Cuts at: 200 1091 1302 1670 2879 3511 3679 4033 200
Size: 891 211 368 1209 632 168 354 2712
Fragments arranged by size:
2712 1209 891 632 368 354 211 168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 201 547 782 1644 1948 2311 2892 3007 4128
Size: 346 235 862 304 363 581 115 1121
Cuts at: 4128 4261 4720 4929 201
Size: 133 459 209 1817
Fragments arranged by size:
1817 1121 862 581 459 363 346 304
235 209 133 115
TatI w'GTAC_w
Cuts at: 6060 6060
Size: 6545
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 321 353 473 505 650 682 1360 1392 2951
Size: 32 120 32 145 32 678 32 1559
Cuts at: 2951 2983 6062 6094 321
Size: 32 3079 32 772
Fragments arranged by size:
3079 1559 772 678 145 120 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4342 4342
Size: 6545
XbaI T'CTAG_A
Cuts at: 3576 3576
Size: 6545
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2911 4040 2911
Size: 1129 5416
XhoI C'TCGA_G
Cuts at: 1921 1921
Size: 6545
XmaI C'CCGG_G
Cuts at: 1067 1894 1900 3565 1067
Size: 827 6 1665 4047
Fragments arranged by size:
4047 1665 827 6
XmnI GAAnn'nnTTC
Cuts at: 2482 2482
Size: 6545
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AleI AlfI AscI BaeI BfrBI BsiWI
BsmI BsrGI BssHII BstZ17I ClaI FspAI HpaI MfeI
NdeI NotI NruI NsiI PacI PshAI PsiI PspXI
PsrI SanDI SexAI SgrAI SnaBI SspI StuI SwaI
ZraI