Restriction map for
pF1KA0185
(Circular) (Six-base) MAPSORT of: pF1KA0185.seq Check: 6211 from: 1 to: 8726
pF1KA0185 8726 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 474 2152 3009 5003 474
Size: 1678 857 1994 4197
Fragments arranged by size:
4197 1994 1678 857
AatII G_ACGT'C
Cuts at: 5549 5549
Size: 8726
AccI GT'mk_AC
Cuts at: 5764 5764
Size: 8726
Acc65I G'GTAC_C
Cuts at: 682 1545 5742 682
Size: 863 4197 3666
Fragments arranged by size:
4197 3666 863
AclI AA'CG_TT
Cuts at: 8566 8566
Size: 8726
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 526 679 1285 1664 3373 3697 4244 4694
Size: 287 153 606 379 1709 324 547 450
Cuts at: 4694 5043 5281 6551 6983 7336 7998 239
Size: 349 238 1270 432 353 662 967
Fragments arranged by size:
1709 1270 967 662 606 547 450 432
379 353 349 324 287 238 153
AfeI AGC'GCT
Cuts at: 5617 5617
Size: 8726
AflII C'TTAA_G
Cuts at: 5044 5044
Size: 8726
AflIII A'CryG_T
Cuts at: 4610 5084 5114 6078 7878 8159 4610
Size: 474 30 964 1800 281 5177
Fragments arranged by size:
5177 1800 964 474 281 30
AhdI GACnn_n'nnGTC
Cuts at: 955 3031 955
Size: 2076 6650
AleI CACnn'nnGTG
Cuts at: 1024 3896 1024
Size: 2872 5854
AlwNI CAG_nnn'CTG
Cuts at: 822 1809 2562 2625 3232 3714 4086 4378 6031
Size: 987 753 63 607 482 372 292 1653
Cuts at: 6031 7469 822
Size: 1438 2079
Fragments arranged by size:
2079 1653 1438 987 753 607 482 372
292 63
ApaI G_GGCC'C
Cuts at: 5043 5043
Size: 8726
ApaLI G'TGCA_C
Cuts at: 1129 2824 7564 1129
Size: 1695 4740 2291
Fragments arranged by size:
4740 2291 1695
ApoI r'AATT_y
Cuts at: 2398 3638 5224 5730 8079 8335 2398
Size: 1240 1586 506 2349 256 2789
Fragments arranged by size:
2789 2349 1586 1240 506 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8726
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8726
AvaI C'yCGr_G
Cuts at: 134 3377 5746 134
Size: 3243 2369 3114
Fragments arranged by size:
3243 3114 2369
BamHI G'GATC_C
Cuts at: 226 1047 2262 3165 4896 5751 226
Size: 821 1215 903 1731 855 3201
Fragments arranged by size:
3201 1731 1215 903 855 821
BanI G'GyrC_C
Cuts at: 682 1545 1812 3331 5302 5742 6156 6404 6439
Size: 863 267 1519 1971 440 414 248 35
Cuts at: 6439 682
Size: 2969
Fragments arranged by size:
2969 1971 1519 863 440 414 267 248
35
BanII G_rGCy'C
Cuts at: 1829 4777 4951 5043 5361 5707 5740 6770 1829
Size: 2948 174 92 318 346 33 1030 3785
Fragments arranged by size:
3785 2948 1030 346 318 174 92 33
BbeI G_GCGC'C
Cuts at: 5306 6160 6408 5306
Size: 854 248 7624
Fragments arranged by size:
7624 854 248
BbsI GAAGACnn'nnnn_
Cuts at: 707 1314 2050 3370 3409 3932 4700 707
Size: 607 736 1320 39 523 768 4733
Fragments arranged by size:
4733 1320 768 736 607 523 39
BbvCI CC'TCA_GC
Cuts at: 650 2872 4379 650
Size: 2222 1507 4997
Fragments arranged by size:
4997 2222 1507
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1169 1203 4638 4672 1169
Size: 34 3435 34 5223
Fragments arranged by size:
5223 3435 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 699 3304 6624 7680 8726 699
Size: 2605 3320 1056 1046 699
Fragments arranged by size:
3320 2605 1056 1046 699
BclI T'GATC_A
Cuts at: 6246 6246
Size: 8726
BglI GCCn_nnn'nGGC
Cuts at: 428 3718 428
Size: 3290 5436
BglII A'GATC_T
Cuts at: 6241 8278 6241
Size: 2037 6689
BlpI GC'TnA_GC
Cuts at: 1294 4945 5470 5838 1294
Size: 3651 525 368 4182
Fragments arranged by size:
4182 3651 525 368
Bme1580I G_kGCm'C
Cuts at: 1133 1483 1817 2717 2828 3334 5043 6351 6444
Size: 350 334 900 111 506 1709 1308 93
Cuts at: 6444 7568 1133
Size: 1124 2291
Fragments arranged by size:
2291 1709 1308 1124 900 506 350 334
111 93
BmgBI CAC'GTC
Cuts at: 4320 4611 4320
Size: 291 8435
BmrI ACTGGGnnnn_n'
Cuts at: 4316 5938 6019 6354 8498 4316
Size: 1622 81 335 2144 4544
Fragments arranged by size:
4544 2144 1622 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3351 4071 7093 3351
Size: 720 3022 4984
Fragments arranged by size:
4984 3022 720
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 409 2080 2140 2584 3502 5128 5383 5704 409
Size: 1671 60 444 918 1626 255 321 3431
Fragments arranged by size:
3431 1671 1626 918 444 321 255 60
Bpu10I CC'TnA_GC
Cuts at: 650 965 1135 2872 4379 650
Size: 315 170 1737 1507 4997
Fragments arranged by size:
4997 1737 1507 315 170
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3031 3625 3808 5437 5902 6781 7495 7793 3031
Size: 594 183 1629 465 879 714 298 3964
Fragments arranged by size:
3964 1629 879 714 594 465 298 183
BsaI GGTCTCn'nnnn_
Cuts at: 2244 4382 8430 2244
Size: 2138 4048 2540
Fragments arranged by size:
4048 2540 2138
BsaAI yAC'GTr
Cuts at: 6079 6079
Size: 8726
BsaBI GATnn'nnATC
Cuts at: 6 6240 8277 6
Size: 6234 2037 455
Fragments arranged by size:
6234 2037 455
BsaHI Gr'CG_yC
Cuts at: 1167 3916 5303 5546 6157 6405 7107 8603 1167
Size: 2749 1387 243 611 248 702 1496 1290
Fragments arranged by size:
2749 1496 1387 1290 702 611 248 243
BsaWI w'CCGG_w
Cuts at: 1584 5252 5915 6436 7114 7525 7672 7946 1584
Size: 3668 663 521 678 411 147 274 2364
Fragments arranged by size:
3668 2364 678 663 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 848 878 1579 1609 3190 3220 3892 3922 5101
Size: 30 701 30 1581 30 672 30 1179
Cuts at: 5101 5131 848
Size: 30 4443
Fragments arranged by size:
4443 1581 1179 701 672 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 152 872 2485 2533 2536 2581 4375 4396 4465
Size: 720 1613 48 3 45 1794 21 69
Cuts at: 4465 4603 4648 4801 4897 5080 5334 152
Size: 138 45 153 96 183 254 3544
Fragments arranged by size:
3544 1794 1613 720 254 183 153 138
96 69 48 45 45 21 3
BseYI C'CCAG_C
Cuts at: 1246 4178 4465 5411 5427 6151 6909 7574 7982
Size: 2932 287 946 16 724 758 665 408
Cuts at: 7982 1246
Size: 1990
Fragments arranged by size:
2932 1990 946 758 724 665 408 287
16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1822 2686 2923 5182 5680 1822
Size: 864 237 2259 498 4868
Fragments arranged by size:
4868 2259 864 498 237
BsiEI CG_ry'CG
Cuts at: 104 6268 6314 7544 104
Size: 6164 46 1230 1286
Fragments arranged by size:
6164 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1133 1829 2828 4777 4951 5476 5707 5740 6518
Size: 696 999 1949 174 525 231 33 778
Cuts at: 6518 6708 7568 1133
Size: 190 860 2291
Fragments arranged by size:
2291 1949 999 860 778 696 525 231
190 174 33
BsmI GAATG_Cn'
Cuts at: 362 551 2300 362
Size: 189 1749 6788
Fragments arranged by size:
6788 1749 189
BsmBI CGTCTCn'nnnn_
Cuts at: 2938 3855 3907 4015 4639 2938
Size: 917 52 108 624 7025
Fragments arranged by size:
7025 917 624 108 52
Bsp1286I G_dGCh'C
Cuts at: 1133 1483 1817 1829 2717 2828 3334 4777 4951
Size: 350 334 12 888 111 506 1443 174
Cuts at: 4951 5043 5361 5476 5707 5740 6351 6444 6518
Size: 92 318 115 231 33 611 93 74
Cuts at: 6518 6708 6770 7568 1133
Size: 190 62 798 2291
Fragments arranged by size:
2291 1443 888 798 611 506 350 334
318 231 190 174 115 111 93 92
74 62 33 12
BspEI T'CCGG_A
Cuts at: 1584 1584
Size: 8726
BspHI T'CATG_A
Cuts at: 1449 2387 7158 1449
Size: 938 4771 3017
Fragments arranged by size:
4771 3017 938
BspMI ACCTGCnnnn'nnnn_
Cuts at: 474 1717 2152 3009 5003 5776 5990 6292 6673
Size: 1243 435 857 1994 773 214 302 381
Cuts at: 6673 474
Size: 2527
Fragments arranged by size:
2527 1994 1243 857 773 435 381 302
214
BsrBI CCG'CTC
Cuts at: 50 5442 5565 7018 7072 8556 8721 50
Size: 5392 123 1453 54 1484 165 55
Fragments arranged by size:
5392 1484 1453 165 123 55 54
BsrDI GCAATG_nn'
Cuts at: 902 2927 5396 6638 902
Size: 2025 2469 1242 2990
Fragments arranged by size:
2990 2469 2025 1242
BsrFI r'CCGG_y
Cuts at: 3096 3550 4576 6724 6905 3096
Size: 454 1026 2148 181 4917
Fragments arranged by size:
4917 2148 1026 454 181
BssSI C'ACGA_G
Cuts at: 6997 7705 6997
Size: 708 8018
BstAPI GCAn_nnn'nTGC
Cuts at: 1103 2484 2580 6103 1103
Size: 1381 96 3523 3726
Fragments arranged by size:
3726 3523 1381 96
BstBI TT'CG_AA
Cuts at: 3444 7087 3444
Size: 3643 5083
BstEII G'GTnAC_C
Cuts at: 2454 2454
Size: 8726
BstXI CCAn_nnnn'nTGG
Cuts at: 1198 1560 5236 5682 1198
Size: 362 3676 446 4242
Fragments arranged by size:
4242 3676 446 362
BstYI r'GATC_y
Cuts at: 1 152 226 1047 2262 3165 4041 4896 5751
Size: 151 74 821 1215 903 876 855 855
Cuts at: 5751 6218 6241 6576 6822 7128 7140 7226 8278
Size: 467 23 335 246 306 12 86 1052
Cuts at: 8278 1
Size: 449
Fragments arranged by size:
1215 1052 903 876 855 855 821 467
449 335 306 246 151 86 74 23
12
Bsu36I CC'TnA_GG
Cuts at: 1331 2355 3715 1331
Size: 1024 1360 6342
Fragments arranged by size:
6342 1360 1024
BtgI C'CryG_G
Cuts at: 107 705 3524 3806 4919 5003 5027 107
Size: 598 2819 282 1113 84 24 3806
Fragments arranged by size:
3806 2819 1113 598 282 84 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2731 6676 6857 7020 2731
Size: 3945 181 163 4437
Fragments arranged by size:
4437 3945 181 163
BtsI GCAGTG_nn'
Cuts at: 754 1091 1211 1475 2494 3371 3494 3968 5969
Size: 337 120 264 1019 877 123 474 2001
Cuts at: 5969 754
Size: 3511
Fragments arranged by size:
3511 2001 1019 877 474 337 264 123
120
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3711 3746 3711
Size: 35 8691
DraI TTT'AAA
Cuts at: 5725 5725
Size: 8726
DraIII CAC_nnn'GTG
Cuts at: 4120 4122 5346 4120
Size: 2 1224 7500
Fragments arranged by size:
7500 1224 2
DrdI GACnn_nn'nnGTC
Cuts at: 3237 6432 7776 3237
Size: 3195 1344 4187
Fragments arranged by size:
4187 3195 1344
EaeI y'GGCC_r
Cuts at: 899 1872 2256 2627 3469 3489 3552 3705 3803
Size: 973 384 371 842 20 63 153 98
Cuts at: 3803 6311 6485 6876 6903 8248 899
Size: 2508 174 391 27 1345 1377
Fragments arranged by size:
2508 1377 1345 973 842 391 384 371
174 153 98 63 27 20
EagI C'GGCC_G
Cuts at: 6311 6311
Size: 8726
EarI CTCTTCn'nnn_
Cuts at: 215 592 2311 4637 6749 6959 215
Size: 377 1719 2326 2112 210 1982
Fragments arranged by size:
2326 2112 1982 1719 377 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6934 7676 7822 8536 6934
Size: 742 146 714 7124
Fragments arranged by size:
7124 742 714 146
EcoICRI GAG'CTC
Cuts at: 1827 4775 4949 5705 5738 1827
Size: 2948 174 756 33 4815
Fragments arranged by size:
4815 2948 756 174 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 409 526 679 1285 1664 2080 2140 2584
Size: 170 117 153 606 379 416 60 444
Cuts at: 2584 3373 3502 3697 4244 4694 5043 5128 5281
Size: 789 129 195 547 450 349 85 153
Cuts at: 5281 5383 5704 6551 6983 7336 7998 239
Size: 102 321 847 432 353 662 967
Fragments arranged by size:
967 847 789 662 606 547 450 444
432 416 379 353 349 321 195 170
153 153 129 117 102 85 60
EcoNI CCTnn'n_nnAGG
Cuts at: 349 2839 4585 5140 349
Size: 2490 1746 555 3935
Fragments arranged by size:
3935 2490 1746 555
EcoO109I rG'GnC_Cy
Cuts at: 510 654 1091 1539 3711 3775 4248 5039 5040
Size: 144 437 448 2172 64 473 791 1
Cuts at: 5040 5679 5865 510
Size: 639 186 3371
Fragments arranged by size:
3371 2172 791 639 473 448 437 186
144 64 1
EcoRI G'AATT_C
Cuts at: 3638 5730 3638
Size: 2092 6634
EcoRV GAT'ATC
Cuts at: 1447 1447
Size: 8726
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1338 2250 3771 4099 4363 5625 6745 1338
Size: 912 1521 328 264 1262 1120 3319
Fragments arranged by size:
3319 1521 1262 1120 912 328 264
FseI GG_CCGG'CC
Cuts at: 3554 3554
Size: 8726
FspI TGC'GCA
Cuts at: 2584 6507 2584
Size: 3923 4803
HaeII r_GCGC'y
Cuts at: 4693 4891 5306 5619 6160 6408 7638 8409 4693
Size: 198 415 313 541 248 1230 771 5010
Fragments arranged by size:
5010 1230 771 541 415 313 248 198
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 941 973 1157 1189 1566 1598 2752 2784 3017
Size: 32 184 32 377 32 1154 32 233
Cuts at: 3017 3049 3892 3924 3944 3976 5485 5517 6568
Size: 32 843 32 20 32 1509 32 1051
Cuts at: 6568 6598 6600 6630 941
Size: 30 2 30 3037
Fragments arranged by size:
3037 1509 1154 1051 843 377 233 184
32 32 32 32 32 32 32 32
30 30 20 2
HincII GTy'rAC
Cuts at: 633 1032 5765 633
Size: 399 4733 3594
Fragments arranged by size:
4733 3594 399
HindIII A'AGCT_T
Cuts at: 126 170 3784 126
Size: 44 3614 5068
Fragments arranged by size:
5068 3614 44
KasI G'GCGC_C
Cuts at: 5302 6156 6404 5302
Size: 854 248 7624
Fragments arranged by size:
7624 854 248
KpnI G_GTAC'C
Cuts at: 686 1549 5746 686
Size: 863 4197 3666
Fragments arranged by size:
4197 3666 863
MluI A'CGCG_T
Cuts at: 8159 8159
Size: 8726
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2096 2486 2657 2798 4823 7485 7669 2096
Size: 390 171 141 2025 2662 184 3153
Fragments arranged by size:
3153 2662 2025 390 184 171 141
MscI TGG'CCA
Cuts at: 1874 2258 3491 3707 3805 6487 1874
Size: 384 1233 216 98 2682 4113
Fragments arranged by size:
4113 2682 1233 384 216 98
MslI CAynn'nnrTG
Cuts at: 1024 1573 3896 5390 6715 6842 1024
Size: 549 2323 1494 1325 127 2908
Fragments arranged by size:
2908 2323 1494 1325 549 127
MspA1I CmG'CkG
Cuts at: 2481 4704 4921 5427 5837 6151 6511 7295 7540
Size: 2223 217 506 410 314 360 784 245
Cuts at: 7540 2481
Size: 3667
Fragments arranged by size:
3667 2223 784 506 410 360 314 245
217
NaeI GCC'GGC
Cuts at: 3098 3552 4578 6907 3098
Size: 454 1026 2329 4917
Fragments arranged by size:
4917 2329 1026 454
NarI GG'CG_CC
Cuts at: 5303 6157 6405 5303
Size: 854 248 7624
Fragments arranged by size:
7624 854 248
NcoI C'CATG_G
Cuts at: 107 705 3524 3806 107
Size: 598 2819 282 5027
Fragments arranged by size:
5027 2819 598 282
NdeI CA'TA_TG
Cuts at: 1503 1503
Size: 8726
NgoMIV G'CCGG_C
Cuts at: 3096 3550 4576 6905 3096
Size: 454 1026 2329 4917
Fragments arranged by size:
4917 2329 1026 454
NspI r_CATG'y
Cuts at: 672 5118 5389 5779 6279 7882 672
Size: 4446 271 390 500 1603 1516
Fragments arranged by size:
4446 1603 1516 500 390 271
PciI A'CATG_T
Cuts at: 5114 7878 5114
Size: 2764 5962
PflMI CCAn_nnn'nTGG
Cuts at: 2625 2847 4970 2625
Size: 222 2123 6381
Fragments arranged by size:
6381 2123 222
PfoI T'CCnGG_A
Cuts at: 2289 3452 4061 5228 5549 2289
Size: 1163 609 1167 321 5466
Fragments arranged by size:
5466 1167 1163 609 321
PmeI GTTT'AAAC
Cuts at: 5725 5725
Size: 8726
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7172 7204 7172
Size: 32 8694
PpuMI rG'GwC_Cy
Cuts at: 510 3775 4248 510
Size: 3265 473 4988
Fragments arranged by size:
4988 3265 473
PshAI GACnn'nnGTC
Cuts at: 2513 5143 2513
Size: 2630 6096
PsiI TTA'TAA
Cuts at: 4208 4208
Size: 8726
PspOMI G'GGCC_C
Cuts at: 5039 5039
Size: 8726
PstI C_TGCA'G
Cuts at: 1739 1977 2576 3975 4703 4979 5000 5334 5354
Size: 238 599 1399 728 276 21 334 20
Cuts at: 5354 5773 6458 1739
Size: 419 685 4007
Fragments arranged by size:
4007 1399 728 685 599 419 334 276
238 21 20
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8726
PvuII CAG'CTG
Cuts at: 4704 5427 6151 6511 4704
Size: 723 724 360 6919
Fragments arranged by size:
6919 724 723 360
RsrII CG'GwC_CG
Cuts at: 6921 6921
Size: 8726
SacI G_AGCT'C
Cuts at: 1829 4777 4951 5707 5740 1829
Size: 2948 174 756 33 4815
Fragments arranged by size:
4815 2948 756 174 33
SacII CC_GC'GG
Cuts at: 4922 4922
Size: 8726
SalI G'TCGA_C
Cuts at: 5763 5763
Size: 8726
SapI GCTCTTCn'nnn_
Cuts at: 2311 6749 6959 2311
Size: 4438 210 4078
Fragments arranged by size:
4438 4078 210
SbfI CC_TGCA'GG
Cuts at: 1977 5000 5773 1977
Size: 3023 773 4930
Fragments arranged by size:
4930 3023 773
ScaI AGT'ACT
Cuts at: 1833 8243 1833
Size: 6410 2316
SexAI A'CCwGG_T
Cuts at: 2720 4274 2720
Size: 1554 7172
SfcI C'TryA_G
Cuts at: 33 1156 1377 1735 1973 2572 3179 3971 4699
Size: 1123 221 358 238 599 607 792 728
Cuts at: 4699 4975 4996 5330 5350 5769 6454 7422 7613
Size: 276 21 334 20 419 685 968 191
Cuts at: 7613 33
Size: 1146
Fragments arranged by size:
1146 1123 968 792 728 685 607 599
419 358 334 276 238 221 191 21
20
SfiI GGCCn_nnn'nGGCC
Cuts at: 3718 3718
Size: 8726
SfoI GGC'GCC
Cuts at: 5304 6158 6406 5304
Size: 854 248 7624
Fragments arranged by size:
7624 854 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8726
SmaI CCC'GGG
Cuts at: 5748 5748
Size: 8726
SmlI C'TyrA_G
Cuts at: 3046 3604 3787 5044 5416 5881 6796 7510 7772
Size: 558 183 1257 372 465 915 714 262
Cuts at: 7772 3046
Size: 4000
Fragments arranged by size:
4000 1257 915 714 558 465 372 262
183
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8726
SphI G_CATG'C
Cuts at: 672 5779 6279 672
Size: 5107 500 3119
Fragments arranged by size:
5107 3119 500
StuI AGG'CCT
Cuts at: 1336 1336
Size: 8726
StyI C'CwwG_G
Cuts at: 107 705 1197 2234 2936 3524 3806 4134 4334
Size: 598 492 1037 702 588 282 328 200
Cuts at: 4334 4661 5141 5675 5860 6214 107
Size: 327 480 534 185 354 2619
Fragments arranged by size:
2619 1037 702 598 588 534 492 480
354 328 327 282 200 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 4949 5079 5493 6309 6442 6901 7110 4949
Size: 130 414 816 133 459 209 6565
Fragments arranged by size:
6565 816 459 414 209 133 130
TatI w'GTAC_w
Cuts at: 773 1831 4613 4979 5165 8241 773
Size: 1058 2782 366 186 3076 1258
Fragments arranged by size:
3076 2782 1258 1058 366 186
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3190 3222 4174 4206 8243 8275 3190
Size: 32 952 32 4037 32 3641
Fragments arranged by size:
4037 3641 952 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1982 2722 3220 3931 3990 6523 1982
Size: 740 498 711 59 2533 4185
Fragments arranged by size:
4185 2533 740 711 498 59
XbaI T'CTAG_A
Cuts at: 3312 5757 3312
Size: 2445 6281
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 2055 2934 3216 3813 6221 114
Size: 1941 879 282 597 2408 2619
Fragments arranged by size:
2619 2408 1941 879 597 282
XmaI C'CCGG_G
Cuts at: 5746 5746
Size: 8726
XmnI GAAnn'nnTTC
Cuts at: 172 1321 3406 5266 5605 172
Size: 1149 2085 1860 339 3293
Fragments arranged by size:
3293 2085 1860 1149 339
ZraI GAC'GTC
Cuts at: 5547 5547
Size: 8726
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AhdI AleI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AgeI AlfI AloI AscI AvrII BaeI BfrBI BmtI
BsiWI BsrGI BssHII BstZ17I ClaI FspAI HpaI MfeI
NheI NotI NruI NsiI PacI PasI PmlI PspXI
PsrI SanDI SgrAI SnaBI SrfI SspI SwaI XhoI