Restriction map for
pF1KA0189
(Circular) (Six-base) MAPSORT of: pF1KA0189.seq Check: 1013 from: 1 to: 6182
pF1KA0189 6182 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AccI GT'mk_AC
Cuts at: 3220 3220
Size: 6182
Acc65I G'GTAC_C
Cuts at: 3198 3198
Size: 6182
AclI AA'CG_TT
Cuts at: 6022 6022
Size: 6182
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1007 1262 4007 4439 4792 5454 1007
Size: 255 2745 432 353 662 1735
Fragments arranged by size:
2745 1735 662 432 353 255
AfeI AGC'GCT
Cuts at: 2608 2608
Size: 6182
AflII C'TTAA_G
Cuts at: 2211 2211
Size: 6182
AflIII A'CryG_T
Cuts at: 1049 3534 5334 5615 1049
Size: 2485 1800 281 1616
Fragments arranged by size:
2485 1800 1616 281
AleI CACnn'nnGTG
Cuts at: 749 2710 3104 749
Size: 1961 394 3827
Fragments arranged by size:
3827 1961 394
AlwNI CAG_nnn'CTG
Cuts at: 1577 1860 1908 1962 2199 2403 2423 2586 2796
Size: 283 48 54 237 204 20 163 210
Cuts at: 2796 2937 3487 4925 1577
Size: 141 550 1438 2834
Fragments arranged by size:
2834 1438 550 283 237 210 204 163
141 54 48 20
ApaI G_GGCC'C
Cuts at: 1236 2639 2763 2790 3163 1236
Size: 1403 124 27 373 4255
Fragments arranged by size:
4255 1403 373 124 27
ApaLI G'TGCA_C
Cuts at: 763 5020 763
Size: 4257 1925
ApoI r'AATT_y
Cuts at: 3186 5535 5791 3186
Size: 2349 256 3577
Fragments arranged by size:
3577 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6182
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6182
AvaI C'yCGr_G
Cuts at: 367 408 1085 3202 367
Size: 41 677 2117 3347
Fragments arranged by size:
3347 2117 677 41
AvrII C'CTAG_G
Cuts at: 2365 2365
Size: 6182
BamHI G'GATC_C
Cuts at: 1952 2940 3207 1952
Size: 988 267 4927
Fragments arranged by size:
4927 988 267
BanI G'GyrC_C
Cuts at: 679 887 1821 2274 2442 2667 2678 2850 3198
Size: 208 934 453 168 225 11 172 348
Cuts at: 3198 3612 3860 3895 679
Size: 414 248 35 2966
Fragments arranged by size:
2966 934 453 414 348 248 225 208
172 168 35 11
BanII G_rGCy'C
Cuts at: 280 554 898 1236 1693 2069 2495 2639 2763
Size: 274 344 338 457 376 426 144 124
Cuts at: 2763 2790 2979 3163 3196 4226 280
Size: 27 189 184 33 1030 2236
Fragments arranged by size:
2236 1030 457 426 376 344 338 274
189 184 144 124 33 27
BbeI G_GCGC'C
Cuts at: 891 2278 3616 3864 891
Size: 1387 1338 248 3209
Fragments arranged by size:
3209 1387 1338 248
BbsI GAAGACnn'nnnn_
Cuts at: 176 176
Size: 6182
BbvCI CC'TCA_GC
Cuts at: 427 663 980 1181 1283 1313 1490 427
Size: 236 317 201 102 30 177 5119
Fragments arranged by size:
5119 317 236 201 177 102 30
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 310 344 310
Size: 34 6148
BciVI GTATCCnnnnn_n'
Cuts at: 4080 5136 6182 4080
Size: 1056 1046 4080
Fragments arranged by size:
4080 1056 1046
BclI T'GATC_A
Cuts at: 2414 3702 2414
Size: 1288 4894
BglI GCCn_nnn'nGGC
Cuts at: 703 867 2351 2504 703
Size: 164 1484 153 4381
Fragments arranged by size:
4381 1484 164 153
BglII A'GATC_T
Cuts at: 2105 3697 5734 2105
Size: 1592 2037 2553
Fragments arranged by size:
2553 2037 1592
BlpI GC'TnA_GC
Cuts at: 959 2489 2494 2519 2603 3294 959
Size: 1530 5 25 84 691 3847
Fragments arranged by size:
3847 1530 691 84 25 5
Bme1580I G_kGCm'C
Cuts at: 402 767 1236 1736 2447 2639 2681 2763 2790
Size: 365 469 500 711 192 42 82 27
Cuts at: 2790 3163 3807 3900 5024 402
Size: 373 644 93 1124 1560
Fragments arranged by size:
1560 1124 711 644 500 469 373 365
192 93 82 42 27
BmgBI CAC'GTC
Cuts at: 2025 2025
Size: 6182
BmrI ACTGGGnnnn_n'
Cuts at: 219 660 2925 2988 3394 3475 3810 5954 219
Size: 441 2265 63 406 81 335 2144 447
Fragments arranged by size:
2265 2144 447 441 406 335 81 63
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 600 4549 600
Size: 3949 2233
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 1027 1133 1262 1434 1501 1663 2086 160
Size: 867 106 129 172 67 162 423 4256
Fragments arranged by size:
4256 867 423 172 162 129 106 67
Bpu10I CC'TnA_GC
Cuts at: 427 663 944 980 1181 1271 1283 1313 1490
Size: 236 281 36 201 90 12 30 177
Cuts at: 1490 2063 2554 427
Size: 573 491 4055
Fragments arranged by size:
4055 573 491 281 236 201 177 90
36 30 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 282 481 3358 4237 4951 5249 282
Size: 199 2877 879 714 298 1215
Fragments arranged by size:
2877 1215 879 714 298 199
BsaI GGTCTCn'nnnn_
Cuts at: 245 588 1088 1972 5886 245
Size: 343 500 884 3914 541
Fragments arranged by size:
3914 884 541 500 343
BsaAI yAC'GTr
Cuts at: 929 1050 1812 1839 3535 929
Size: 121 762 27 1696 3576
Fragments arranged by size:
3576 1696 762 121 27
BsaBI GATnn'nnATC
Cuts at: 6 2774 3696 5733 6
Size: 2768 922 2037 455
Fragments arranged by size:
2768 2037 922 455
BsaHI Gr'CG_yC
Cuts at: 888 2275 3613 3861 4563 6059 888
Size: 1387 1338 248 702 1496 1011
Fragments arranged by size:
1496 1387 1338 1011 702 248
BsaWI w'CCGG_w
Cuts at: 2670 2943 3371 3892 4570 4981 5128 5402 2670
Size: 273 428 521 678 411 147 274 3450
Fragments arranged by size:
3450 678 521 428 411 274 273 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 415 1018 1141 1144 1437 1791 2107 415
Size: 603 123 3 293 354 316 4490
Fragments arranged by size:
4490 603 354 316 293 123 3
BseYI C'CCAG_C
Cuts at: 403 954 1224 1326 1344 1621 1748 2508 2639
Size: 551 270 102 18 277 127 760 131
Cuts at: 2639 2724 3607 4365 5030 5438 403
Size: 85 883 758 665 408 1147
Fragments arranged by size:
1147 883 760 758 665 551 408 277
270 131 127 102 85 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1666 1861 2490 1666
Size: 195 629 5358
Fragments arranged by size:
5358 629 195
BsiEI CG_ry'CG
Cuts at: 104 3724 3770 5000 104
Size: 3620 46 1230 1286
Fragments arranged by size:
3620 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 767 2495 3196 3974 4164 5024 767
Size: 1728 701 778 190 860 1925
Fragments arranged by size:
1925 1728 860 778 701 190
BsmI GAATG_Cn'
Cuts at: 1871 1871
Size: 6182
Bsp1286I G_dGCh'C
Cuts at: 280 402 554 767 898 1236 1693 1736 2069
Size: 122 152 213 131 338 457 43 333
Cuts at: 2069 2447 2495 2639 2681 2763 2790 2979 3163
Size: 378 48 144 42 82 27 189 184
Cuts at: 3163 3196 3807 3900 3974 4164 4226 5024 280
Size: 33 611 93 74 190 62 798 1438
Fragments arranged by size:
1438 798 611 457 378 338 333 213
190 189 184 152 144 131 122 93
82 74 62 48 43 42 33 27
BspEI T'CCGG_A
Cuts at: 2943 2943
Size: 6182
BspHI T'CATG_A
Cuts at: 1772 4614 1772
Size: 2842 3340
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1970 2042 2066 2594 2909 3232 3446 3748 4129
Size: 72 24 528 315 323 214 302 381
Cuts at: 4129 1970
Size: 4023
Fragments arranged by size:
4023 528 381 323 315 302 214 72
24
BsrBI CCG'CTC
Cuts at: 50 2757 3026 4474 4528 6012 6177 50
Size: 2707 269 1448 54 1484 165 55
Fragments arranged by size:
2707 1484 1448 269 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2219 4094 2219
Size: 1875 4307
BsrFI r'CCGG_y
Cuts at: 416 667 1652 4180 4361 416
Size: 251 985 2528 181 2237
Fragments arranged by size:
2528 2237 985 251 181
BssSI C'ACGA_G
Cuts at: 2906 4453 5161 2906
Size: 1547 708 3927
Fragments arranged by size:
3927 1547 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1884 1904 2685 3559 1884
Size: 20 781 874 4507
Fragments arranged by size:
4507 874 781 20
BstBI TT'CG_AA
Cuts at: 4543 4543
Size: 6182
BstXI CCAn_nnnn'nTGG
Cuts at: 2712 3117 2712
Size: 405 5777
BstYI r'GATC_y
Cuts at: 1 1952 2105 2775 2940 3125 3207 3674 3697
Size: 1951 153 670 165 185 82 467 23
Cuts at: 3697 4032 4278 4584 4596 4682 5734 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1951 1052 670 467 449 335 306 246
185 165 153 86 82 23 12
Bsu36I CC'TnA_GG
Cuts at: 830 3058 830
Size: 2228 3954
BtgI C'CryG_G
Cuts at: 1212 3110 1212
Size: 1898 4284
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4132 4313 4476 4132
Size: 181 163 5838
Fragments arranged by size:
5838 181 163
BtsI GCAGTG_nn'
Cuts at: 196 681 1693 1858 2270 3425 196
Size: 485 1012 165 412 1155 2953
Fragments arranged by size:
2953 1155 1012 485 412 165
DraI TTT'AAA
Cuts at: 3181 3181
Size: 6182
DrdI GACnn_nn'nnGTC
Cuts at: 3888 5232 3888
Size: 1344 4838
EaeI y'GGCC_r
Cuts at: 3767 3941 4332 4359 5704 3767
Size: 174 391 27 1345 4245
Fragments arranged by size:
4245 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3767 3767
Size: 6182
EarI CTCTTCn'nnn_
Cuts at: 173 179 1118 1301 2564 4205 4415 173
Size: 6 939 183 1263 1641 210 1940
Fragments arranged by size:
1940 1641 1263 939 210 183 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 534 4390 5132 5278 5992 534
Size: 3856 742 146 714 724
Fragments arranged by size:
3856 742 724 714 146
EcoICRI GAG'CTC
Cuts at: 2493 3194 2493
Size: 701 5481
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 1007 1027 1133 1262 1434 1501 1663 2086
Size: 847 20 106 129 172 67 162 423
Cuts at: 2086 4007 4439 4792 5454 160
Size: 1921 432 353 662 888
Fragments arranged by size:
1921 888 847 662 432 423 353 172
162 129 106 67 20
EcoNI CCTnn'n_nnAGG
Cuts at: 1684 2122 2322 1684
Size: 438 200 5544
Fragments arranged by size:
5544 438 200
EcoO109I rG'GnC_Cy
Cuts at: 251 451 1108 1317 1502 1559 2055 2362 2370
Size: 200 657 209 185 57 496 307 8
Cuts at: 2370 2400 2583 2635 2636 2760 3160 3321 251
Size: 30 183 52 1 124 400 161 3112
Fragments arranged by size:
3112 657 496 400 307 209 200 185
183 161 124 57 52 30 8 1
EcoRI G'AATT_C
Cuts at: 3186 3186
Size: 6182
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4201 4201
Size: 6182
FspI TGC'GCA
Cuts at: 1897 3963 1897
Size: 2066 4116
HaeII r_GCGC'y
Cuts at: 477 891 2278 2610 2895 3616 3864 5094 5865
Size: 414 1387 332 285 721 248 1230 771
Cuts at: 5865 477
Size: 794
Fragments arranged by size:
1387 1230 794 771 721 414 332 285
248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 810 842 1066 1098 1996 2028 2209 2241 2302
Size: 32 224 32 898 32 181 32 61
Cuts at: 2302 2334 4024 4054 4056 4086 810
Size: 32 1690 30 2 30 2906
Fragments arranged by size:
2906 1690 898 224 181 61 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3221 3221
Size: 6182
KasI G'GCGC_C
Cuts at: 887 2274 3612 3860 887
Size: 1387 1338 248 3209
Fragments arranged by size:
3209 1387 1338 248
KpnI G_GTAC'C
Cuts at: 3202 3202
Size: 6182
MluI A'CGCG_T
Cuts at: 5615 5615
Size: 6182
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4941 5125 4941
Size: 184 5998
MscI TGG'CCA
Cuts at: 3943 3943
Size: 6182
MslI CAynn'nnrTG
Cuts at: 352 749 1837 2294 2710 3104 3115 4171 4298
Size: 397 1088 457 416 394 11 1056 127
Cuts at: 4298 352
Size: 2236
Fragments arranged by size:
2236 1088 1056 457 416 397 394 127
11
MspA1I CmG'CkG
Cuts at: 628 958 1282 2466 2488 2728 3157 3293 3607
Size: 330 324 1184 22 240 429 136 314
Cuts at: 3607 3967 4751 4996 628
Size: 360 784 245 1814
Fragments arranged by size:
1814 1184 784 429 360 330 324 314
245 240 136 22
NaeI GCC'GGC
Cuts at: 4363 4363
Size: 6182
NarI GG'CG_CC
Cuts at: 888 2275 3613 3861 888
Size: 1387 1338 248 3209
Fragments arranged by size:
3209 1387 1338 248
NcoI C'CATG_G
Cuts at: 1212 3110 1212
Size: 1898 4284
NgoMIV G'CCGG_C
Cuts at: 4361 4361
Size: 6182
NspI r_CATG'y
Cuts at: 3235 3735 5338 3235
Size: 500 1603 4079
Fragments arranged by size:
4079 1603 500
PasI CC'CwG_GG
Cuts at: 438 2397 438
Size: 1959 4223
PciI A'CATG_T
Cuts at: 5334 5334
Size: 6182
PflMI CCAn_nnn'nTGG
Cuts at: 650 1908 2454 2796 2937 650
Size: 1258 546 342 141 3895
Fragments arranged by size:
3895 1258 546 342 141
PfoI T'CCnGG_A
Cuts at: 1948 2051 2579 2750 3128 1948
Size: 103 528 171 378 5002
Fragments arranged by size:
5002 528 378 171 103
PmeI GTTT'AAAC
Cuts at: 3181 3181
Size: 6182
PmlI CAC'GTG
Cuts at: 1050 1839 1050
Size: 789 5393
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4628 4660 4628
Size: 32 6150
PpuMI rG'GwC_Cy
Cuts at: 251 451 1502 2055 2370 2583 251
Size: 200 1051 553 315 213 3850
Fragments arranged by size:
3850 1051 553 315 213 200
PspOMI G'GGCC_C
Cuts at: 1232 2635 2759 2786 3159 1232
Size: 1403 124 27 373 4255
Fragments arranged by size:
4255 1403 373 124 27
PspXI vC'TCGA_Gb
Cuts at: 408 408
Size: 6182
PstI C_TGCA'G
Cuts at: 1582 2465 2544 3152 3229 3914 1582
Size: 883 79 608 77 685 3850
Fragments arranged by size:
3850 883 685 608 79 77
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6182
PvuII CAG'CTG
Cuts at: 628 958 1282 2466 2488 2728 3607 3967 628
Size: 330 324 1184 22 240 879 360 2843
Fragments arranged by size:
2843 1184 879 360 330 324 240 22
RsrII CG'GwC_CG
Cuts at: 4377 4377
Size: 6182
SacI G_AGCT'C
Cuts at: 2495 3196 2495
Size: 701 5481
SalI G'TCGA_C
Cuts at: 3219 3219
Size: 6182
SanDI GG'GwC_CC
Cuts at: 451 1502 451
Size: 1051 5131
SapI GCTCTTCn'nnn_
Cuts at: 179 4205 4415 179
Size: 4026 210 1946
Fragments arranged by size:
4026 1946 210
SbfI CC_TGCA'GG
Cuts at: 1582 3152 3229 1582
Size: 1570 77 4535
Fragments arranged by size:
4535 1570 77
ScaI AGT'ACT
Cuts at: 5699 5699
Size: 6182
SfcI C'TryA_G
Cuts at: 33 1060 1578 2191 2461 2478 2540 3148 3225
Size: 1027 518 613 270 17 62 608 77
Cuts at: 3225 3910 4878 5069 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 1027 968 685 613 608 518 270
191 77 62 17
SfiI GGCCn_nnn'nGGCC
Cuts at: 703 703
Size: 6182
SfoI GGC'GCC
Cuts at: 889 2276 3614 3862 889
Size: 1387 1338 248 3209
Fragments arranged by size:
3209 1387 1338 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6182
SmaI CCC'GGG
Cuts at: 3204 3204
Size: 6182
SmlI C'TyrA_G
Cuts at: 297 408 496 2211 3337 4252 4966 5228 297
Size: 111 88 1715 1126 915 714 262 1251
Fragments arranged by size:
1715 1251 1126 915 714 262 111 88
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6182
SphI G_CATG'C
Cuts at: 3235 3735 3235
Size: 500 5682
StuI AGG'CCT
Cuts at: 697 706 697
Size: 9 6173
StyI C'CwwG_G
Cuts at: 1212 2123 2365 3110 3316 3670 1212
Size: 911 242 745 206 354 3724
Fragments arranged by size:
3724 911 745 354 242 206
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 451 2410 3765 3898 4357 4566 451
Size: 1959 1355 133 459 209 2067
Fragments arranged by size:
2067 1959 1355 459 209 133
TatI w'GTAC_w
Cuts at: 1724 5697 1724
Size: 3973 2209
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 175 207 217 249 1817 1849 5699 5731 175
Size: 32 10 32 1568 32 3850 32 626
Fragments arranged by size:
3850 1568 626 32 32 32 32 10
Tth111I GACn'n_nGTC
Cuts at: 1993 3979 1993
Size: 1986 4196
XbaI T'CTAG_A
Cuts at: 3213 3213
Size: 6182
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 869 3677 869
Size: 2808 3374
XhoI C'TCGA_G
Cuts at: 408 408
Size: 6182
XmaI C'CCGG_G
Cuts at: 3202 3202
Size: 6182
XmnI GAAnn'nnTTC
Cuts at: 2434 2434
Size: 6182
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AgeI AhdI AlfI AloI AscI BaeI
BfrBI BmtI BsaXI BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PshAI PsiI PsrI SacII SexAI SgrAI SnaBI SrfI
SspI SwaI ZraI