Restriction map for
pF1KA0196
(Circular) (Six-base) MAPSORT of: pF1KA0196.seq Check: 6838 from: 1 to: 6590
pF1KA0196 6590 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AccI GT'mk_AC
Cuts at: 428 789 2574 3628 428
Size: 361 1785 1054 3390
Fragments arranged by size:
3390 1785 1054 361
Acc65I G'GTAC_C
Cuts at: 3606 3606
Size: 6590
AclI AA'CG_TT
Cuts at: 2563 6430 2563
Size: 3867 2723
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2023 2180 2428 3379 3557 4415 4847 5200 5862
Size: 157 248 951 178 858 432 353 662
Cuts at: 5862 2023
Size: 2751
Fragments arranged by size:
2751 951 858 662 432 353 248 178
157
AfeI AGC'GCT
Cuts at: 3211 3211
Size: 6590
AflIII A'CryG_T
Cuts at: 2617 3543 3942 5742 6023 2617
Size: 926 399 1800 281 3184
Fragments arranged by size:
3184 1800 926 399 281
AlwNI CAG_nnn'CTG
Cuts at: 1347 2223 2976 3393 3895 5333 1347
Size: 876 753 417 502 1438 2604
Fragments arranged by size:
2604 1438 876 753 502 417
ApaLI G'TGCA_C
Cuts at: 5428 5428
Size: 6590
ApoI r'AATT_y
Cuts at: 355 1636 2775 2880 2891 3458 3594 5943 6199
Size: 1281 1139 105 11 567 136 2349 256
Cuts at: 6199 355
Size: 746
Fragments arranged by size:
2349 1281 1139 746 567 256 136 105
11
AseI AT'TA_AT
Cuts at: 20 1907 20
Size: 1887 4703
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6590
AvaI C'yCGr_G
Cuts at: 730 2297 2941 3610 730
Size: 1567 644 669 3710
Fragments arranged by size:
3710 1567 669 644
BamHI G'GATC_C
Cuts at: 1331 2208 3615 1331
Size: 877 1407 4306
Fragments arranged by size:
4306 1407 877
BanI G'GyrC_C
Cuts at: 706 3478 3606 4020 4268 4303 706
Size: 2772 128 414 248 35 2993
Fragments arranged by size:
2993 2772 414 248 128 35
BanII G_rGCy'C
Cuts at: 3604 4634 3604
Size: 1030 5560
BbeI G_GCGC'C
Cuts at: 4024 4272 4024
Size: 248 6342
BbvCI CC'TCA_GC
Cuts at: 2089 2089
Size: 6590
BciVI GTATCCnnnnn_n'
Cuts at: 839 1347 1977 4488 5544 6590 839
Size: 508 630 2511 1056 1046 839
Fragments arranged by size:
2511 1056 1046 839 630 508
BclI T'GATC_A
Cuts at: 246 1709 1742 4110 246
Size: 1463 33 2368 2726
Fragments arranged by size:
2726 2368 1463 33
BglII A'GATC_T
Cuts at: 796 1541 4105 6142 796
Size: 745 2564 2037 1244
Fragments arranged by size:
2564 2037 1244 745
BlpI GC'TnA_GC
Cuts at: 326 1933 2102 3702 326
Size: 1607 169 1600 3214
Fragments arranged by size:
3214 1607 1600 169
Bme1580I G_kGCm'C
Cuts at: 3483 4215 4308 5432 3483
Size: 732 93 1124 4641
Fragments arranged by size:
4641 1124 732 93
BmrI ACTGGGnnnn_n'
Cuts at: 3802 3883 4218 6362 3802
Size: 81 335 2144 4030
Fragments arranged by size:
4030 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4957 4957
Size: 6590
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1976 3067 3178 3514 1976
Size: 1091 111 336 5052
Fragments arranged by size:
5052 1091 336 111
Bpu10I CC'TnA_GC
Cuts at: 2089 2089
Size: 6590
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1109 3766 4645 5359 5657 1109
Size: 2657 879 714 298 2042
Fragments arranged by size:
2657 2042 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 825 6294 825
Size: 5469 1121
BsaAI yAC'GTr
Cuts at: 885 3943 885
Size: 3058 3532
BsaBI GATnn'nnATC
Cuts at: 6 4104 6141 6
Size: 4098 2037 455
Fragments arranged by size:
4098 2037 455
BsaHI Gr'CG_yC
Cuts at: 4021 4269 4971 6467 4021
Size: 248 702 1496 4144
Fragments arranged by size:
4144 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3779 4300 4978 5389 5536 5810 3779
Size: 521 678 411 147 274 4559
Fragments arranged by size:
4559 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1949 1979 2446 2476 1949
Size: 30 467 30 6063
Fragments arranged by size:
6063 467 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1177 1747 1177
Size: 570 6020
BseYI C'CCAG_C
Cuts at: 4015 4773 5438 5846 4015
Size: 758 665 408 4759
Fragments arranged by size:
4759 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1150 1343 1150
Size: 193 6397
BsiEI CG_ry'CG
Cuts at: 104 3311 4132 4178 5408 104
Size: 3207 821 46 1230 1286
Fragments arranged by size:
3207 1286 1230 821 46
BsiHKAI G_wGCw'C
Cuts at: 3604 4382 4572 5432 3604
Size: 778 190 860 4762
Fragments arranged by size:
4762 860 778 190
BsmI GAATG_Cn'
Cuts at: 2853 3170 2853
Size: 317 6273
Bsp1286I G_dGCh'C
Cuts at: 3483 3604 4215 4308 4382 4572 4634 5432 3483
Size: 121 611 93 74 190 62 798 4641
Fragments arranged by size:
4641 798 611 190 121 93 74 62
BspHI T'CATG_A
Cuts at: 2843 5022 2843
Size: 2179 4411
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1937 3640 3854 4156 4537 1937
Size: 1703 214 302 381 3990
Fragments arranged by size:
3990 1703 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1452 4882 4936 6420 6585 50
Size: 1402 3430 54 1484 165 55
Fragments arranged by size:
3430 1484 1402 165 55 54
BsrDI GCAATG_nn'
Cuts at: 182 1221 2906 4502 182
Size: 1039 1685 1596 2270
Fragments arranged by size:
2270 1685 1596 1039
BsrFI r'CCGG_y
Cuts at: 4588 4769 4588
Size: 181 6409
BsrGI T'GTAC_A
Cuts at: 411 1602 3436 411
Size: 1191 1834 3565
Fragments arranged by size:
3565 1834 1191
BssSI C'ACGA_G
Cuts at: 4861 5569 4861
Size: 708 5882
BstAPI GCAn_nnn'nTGC
Cuts at: 1042 3967 1042
Size: 2925 3665
BstBI TT'CG_AA
Cuts at: 678 4951 678
Size: 4273 2317
BstXI CCAn_nnnn'nTGG
Cuts at: 305 968 305
Size: 663 5927
BstYI r'GATC_y
Cuts at: 1 796 1331 1541 2208 3615 4082 4105 4440
Size: 795 535 210 667 1407 467 23 335
Cuts at: 4440 4686 4992 5004 5090 6142 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1407 1052 795 667 535 467 449 335
306 246 210 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 154 154
Size: 6590
BtgI C'CryG_G
Cuts at: 1838 2348 3422 1838
Size: 510 1074 5006
Fragments arranged by size:
5006 1074 510
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 829 4540 4721 4884 829
Size: 3711 181 163 2535
Fragments arranged by size:
3711 2535 181 163
BtsI GCAGTG_nn'
Cuts at: 3439 3833 3439
Size: 394 6196
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 120 155 120
Size: 35 6555
DraI TTT'AAA
Cuts at: 1049 1565 2834 3589 1049
Size: 516 1269 755 4050
Fragments arranged by size:
4050 1269 755 516
DrdI GACnn_nn'nnGTC
Cuts at: 4296 5640 4296
Size: 1344 5246
EaeI y'GGCC_r
Cuts at: 141 3325 3405 4175 4349 4740 4767 6112 141
Size: 3184 80 770 174 391 27 1345 619
Fragments arranged by size:
3184 1345 770 619 391 174 80 27
EagI C'GGCC_G
Cuts at: 4175 4175
Size: 6590
EarI CTCTTCn'nnn_
Cuts at: 1342 1619 2024 2692 4613 4823 1342
Size: 277 405 668 1921 210 3109
Fragments arranged by size:
3109 1921 668 405 277 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2892 4798 5540 5686 6400 2892
Size: 1906 742 146 714 3082
Fragments arranged by size:
3082 1906 742 714 146
EcoICRI GAG'CTC
Cuts at: 3602 3602
Size: 6590
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1976 2023 2180 2428 3067 3178 3379 3514 3557
Size: 47 157 248 639 111 201 135 43
Cuts at: 3557 4415 4847 5200 5862 1976
Size: 858 432 353 662 2704
Fragments arranged by size:
2704 858 662 639 432 353 248 201
157 135 111 47 43
EcoO109I rG'GnC_Cy
Cuts at: 1764 3096 3729 1764
Size: 1332 633 4625
Fragments arranged by size:
4625 1332 633
EcoRI G'AATT_C
Cuts at: 1636 3594 1636
Size: 1958 4632
EcoRV GAT'ATC
Cuts at: 267 442 267
Size: 175 6415
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4609 4609
Size: 6590
FspI TGC'GCA
Cuts at: 4371 4371
Size: 6590
HaeII r_GCGC'y
Cuts at: 3213 3400 4024 4272 5502 6273 3213
Size: 187 624 248 1230 771 3530
Fragments arranged by size:
3530 1230 771 624 248 187
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 740 772 1951 1983 2922 2954 4432 4462 4464
Size: 32 1179 32 939 32 1478 30 2
Cuts at: 4464 4494 740
Size: 30 2836
Fragments arranged by size:
2836 1478 1179 939 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 790 2394 2575 3629 790
Size: 1604 181 1054 3751
Fragments arranged by size:
3751 1604 1054 181
HindIII A'AGCT_T
Cuts at: 1189 1526 1616 1870 2035 2131 1189
Size: 337 90 254 165 96 5648
Fragments arranged by size:
5648 337 254 165 96 90
KasI G'GCGC_C
Cuts at: 4020 4268 4020
Size: 248 6342
KpnI G_GTAC'C
Cuts at: 3610 3610
Size: 6590
MfeI C'AATT_G
Cuts at: 3227 3227
Size: 6590
MluI A'CGCG_T
Cuts at: 6023 6023
Size: 6590
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 92 746 1331 1628 5349 5533 92
Size: 654 585 297 3721 184 1149
Fragments arranged by size:
3721 1149 654 585 297 184
MscI TGG'CCA
Cuts at: 143 3327 3407 4351 143
Size: 3184 80 944 2382
Fragments arranged by size:
3184 2382 944 80
MslI CAynn'nnrTG
Cuts at: 775 2170 2203 4579 4706 775
Size: 1395 33 2376 127 2659
Fragments arranged by size:
2659 2376 1395 127 33
MspA1I CmG'CkG
Cuts at: 1344 2634 3040 3166 3701 4015 4375 5159 5404
Size: 1290 406 126 535 314 360 784 245
Cuts at: 5404 1344
Size: 2530
Fragments arranged by size:
2530 1290 784 535 406 360 314 245
126
NaeI GCC'GGC
Cuts at: 4771 4771
Size: 6590
NarI GG'CG_CC
Cuts at: 4021 4269 4021
Size: 248 6342
NcoI C'CATG_G
Cuts at: 1838 2348 1838
Size: 510 6080
NgoMIV G'CCGG_C
Cuts at: 4769 4769
Size: 6590
NspI r_CATG'y
Cuts at: 1123 2010 2508 2621 3547 3643 4143 5746 1123
Size: 887 498 113 926 96 500 1603 1967
Fragments arranged by size:
1967 1603 926 887 500 498 113 96
PciI A'CATG_T
Cuts at: 2617 3543 5742 2617
Size: 926 2199 3465
Fragments arranged by size:
3465 2199 926
PflMI CCAn_nnn'nTGG
Cuts at: 1229 1647 1229
Size: 418 6172
PfoI T'CCnGG_A
Cuts at: 3491 3491
Size: 6590
PmeI GTTT'AAAC
Cuts at: 3589 3589
Size: 6590
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5036 5068 5036
Size: 32 6558
PpuMI rG'GwC_Cy
Cuts at: 1764 3096 1764
Size: 1332 5258
PspXI vC'TCGA_Gb
Cuts at: 2297 2297
Size: 6590
PstI C_TGCA'G
Cuts at: 2584 3468 3637 4322 2584
Size: 884 169 685 4852
Fragments arranged by size:
4852 884 685 169
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6590
PvuII CAG'CTG
Cuts at: 1344 2634 3040 3166 4015 4375 1344
Size: 1290 406 126 849 360 3559
Fragments arranged by size:
3559 1290 849 406 360 126
RsrII CG'GwC_CG
Cuts at: 4785 4785
Size: 6590
SacI G_AGCT'C
Cuts at: 3604 3604
Size: 6590
SalI G'TCGA_C
Cuts at: 788 2573 3627 788
Size: 1785 1054 3751
Fragments arranged by size:
3751 1785 1054
SapI GCTCTTCn'nnn_
Cuts at: 4613 4823 4613
Size: 210 6380
SbfI CC_TGCA'GG
Cuts at: 3637 3637
Size: 6590
ScaI AGT'ACT
Cuts at: 1948 6107 1948
Size: 4159 2431
SexAI A'CCwGG_T
Cuts at: 702 815 702
Size: 113 6477
SfcI C'TryA_G
Cuts at: 33 337 2580 3464 3633 4318 5286 5477 33
Size: 304 2243 884 169 685 968 191 1146
Fragments arranged by size:
2243 1146 968 884 685 304 191 169
SfoI GGC'GCC
Cuts at: 4022 4270 4022
Size: 248 6342
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6590
SmaI CCC'GGG
Cuts at: 3612 3612
Size: 6590
SmlI C'TyrA_G
Cuts at: 1124 2297 2941 3745 4660 5374 5636 1124
Size: 1173 644 804 915 714 262 2078
Fragments arranged by size:
2078 1173 915 804 714 644 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6590
SphI G_CATG'C
Cuts at: 1123 3643 4143 1123
Size: 2520 500 3570
Fragments arranged by size:
3570 2520 500
SspI AAT'ATT
Cuts at: 1556 1556
Size: 6590
StyI C'CwwG_G
Cuts at: 1838 2348 2708 3724 4078 1838
Size: 510 360 1016 354 4350
Fragments arranged by size:
4350 1016 510 360 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 934 3325 3466 4173 4306 4765 4974 934
Size: 2391 141 707 133 459 209 2550
Fragments arranged by size:
2550 2391 707 459 209 141 133
TatI w'GTAC_w
Cuts at: 411 536 682 1602 1946 3436 6105 411
Size: 125 146 920 344 1490 2669 896
Fragments arranged by size:
2669 1490 920 896 344 146 125
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2446 2478 2508 2540 3320 3352 6107 6139 2446
Size: 32 30 32 780 32 2755 32 2897
Fragments arranged by size:
2897 2755 780 32 32 32 32 30
Tth111I GACn'n_nGTC
Cuts at: 2389 4387 2389
Size: 1998 4592
XbaI T'CTAG_A
Cuts at: 444 3621 444
Size: 3177 3413
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4085 4085
Size: 6590
XhoI C'TCGA_G
Cuts at: 2297 2941 2297
Size: 644 5946
XmaI C'CCGG_G
Cuts at: 3610 3610
Size: 6590
XmnI GAAnn'nnTTC
Cuts at: 356 1520 1628 3364 356
Size: 1164 108 1736 3582
Fragments arranged by size:
3582 1736 1164 108
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BcgI BfrBI BglI
BmgBI BmtI BsiWI BsmBI BspEI BssHII BstEII BstZ17I
ClaI DraIII EcoNI FseI FspAI HpaI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PshAI PsiI
PspOMI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
StuI SwaI ZraI