Restriction map for
pF1KA0197
(Circular) (Six-base) MAPSORT of: pF1KA0197.seq Check: 7960 from: 1 to: 7058
pF1KA0197 7058 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 2869 4096 2869
Size: 1227 5831
Acc65I G'GTAC_C
Cuts at: 1691 4074 1691
Size: 2383 4675
AclI AA'CG_TT
Cuts at: 6898 6898
Size: 7058
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 830 1504 1594 2828 2987 4883 5315 6062 6330
Size: 674 90 1234 159 1896 432 747 268
Cuts at: 6330 830
Size: 1558
Fragments arranged by size:
1896 1558 1234 747 674 432 268 159
90
AflII C'TTAA_G
Cuts at: 724 724
Size: 7058
AflIII A'CryG_T
Cuts at: 3007 4410 5514 6491 3007
Size: 1403 1104 977 3574
Fragments arranged by size:
3574 1403 1104 977
AgeI A'CCGG_T
Cuts at: 5446 5446
Size: 7058
AleI CACnn'nnGTG
Cuts at: 3926 3926
Size: 7058
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2176 3775 2176
Size: 1599 5459
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 704 736 704
Size: 32 7026
AlwNI CAG_nnn'CTG
Cuts at: 625 1320 1855 1929 2220 2422 2806 3442 3622
Size: 695 535 74 291 202 384 636 180
Cuts at: 3622 4363 5930 625
Size: 741 1567 1753
Fragments arranged by size:
1753 1567 741 695 636 535 384 291
202 180 74
ApaI G_GGCC'C
Cuts at: 1227 1227
Size: 7058
ApaLI G'TGCA_C
Cuts at: 484 5828 484
Size: 5344 1714
ApoI r'AATT_y
Cuts at: 184 394 1458 1543 1566 1579 2824 3071 4062
Size: 210 1064 85 23 13 1245 247 991
Cuts at: 4062 6411 6667 184
Size: 2349 256 575
Fragments arranged by size:
2349 1245 1064 991 575 256 247 210
85 23 13
AseI AT'TA_AT
Cuts at: 20 20
Size: 7058
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7058
AvaI C'yCGr_G
Cuts at: 1802 4078 1802
Size: 2276 4782
BamHI G'GATC_C
Cuts at: 4083 4083
Size: 7058
BanI G'GyrC_C
Cuts at: 226 1691 2535 4074 4488 4736 4771 226
Size: 1465 844 1539 414 248 35 2513
Fragments arranged by size:
2513 1539 1465 844 414 248 35
BanII G_rGCy'C
Cuts at: 123 1227 4072 5102 123
Size: 1104 2845 1030 2079
Fragments arranged by size:
2845 2079 1104 1030
BbeI G_GCGC'C
Cuts at: 230 4492 4740 230
Size: 4262 248 2548
Fragments arranged by size:
4262 2548 248
BbsI GAAGACnn'nnnn_
Cuts at: 325 1600 325
Size: 1275 5783
BciVI GTATCCnnnnn_n'
Cuts at: 4956 5717 7058 4956
Size: 761 1341 4956
Fragments arranged by size:
4956 1341 761
BclI T'GATC_A
Cuts at: 1350 1940 4578 1350
Size: 590 2638 3830
Fragments arranged by size:
3830 2638 590
BfrBI ATG'CAT
Cuts at: 1083 3752 1083
Size: 2669 4389
BglI GCCn_nnn'nGGC
Cuts at: 112 3972 112
Size: 3860 3198
BglII A'GATC_T
Cuts at: 2199 3546 4573 6610 2199
Size: 1347 1027 2037 2647
Fragments arranged by size:
2647 2037 1347 1027
BlpI GC'TnA_GC
Cuts at: 145 155 664 2084 2276 3593 4170 145
Size: 10 509 1420 192 1317 577 3033
Fragments arranged by size:
3033 1420 1317 577 509 192 10
Bme1580I G_kGCm'C
Cuts at: 488 1227 4683 4776 5832 488
Size: 739 3456 93 1056 1714
Fragments arranged by size:
3456 1714 1056 739 93
BmrI ACTGGGnnnn_n'
Cuts at: 4270 4351 4686 6830 4270
Size: 81 335 2144 4498
Fragments arranged by size:
4498 2144 335 81
BmtI G_CTAG'C
Cuts at: 3808 3808
Size: 7058
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5425 5425
Size: 7058
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 347 440 997 1494 2398 3485 3571 3910 347
Size: 93 557 497 904 1087 86 339 3495
Fragments arranged by size:
3495 1087 904 557 497 339 93 86
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3376 4234 5113 5605 5903 3376
Size: 858 879 492 298 4531
Fragments arranged by size:
4531 879 858 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 6762 6762
Size: 7058
BsaAI yAC'GTr
Cuts at: 267 3208 4411 267
Size: 2941 1203 2914
Fragments arranged by size:
2941 2914 1203
BsaBI GATnn'nnATC
Cuts at: 6 1339 4572 6609 6
Size: 1333 3233 2037 455
Fragments arranged by size:
3233 2037 1333 455
BsaHI Gr'CG_yC
Cuts at: 227 4489 4737 5439 6935 227
Size: 4262 248 702 1496 350
Fragments arranged by size:
4262 1496 702 350 248
BsaWI w'CCGG_w
Cuts at: 1905 4247 4768 5446 5720 5867 6278 1905
Size: 2342 521 678 274 147 411 2685
Fragments arranged by size:
2685 2342 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 670 700 937 967 2228 2258 2851 2881 3544
Size: 30 237 30 1261 30 593 30 663
Cuts at: 3544 3574 670
Size: 30 4154
Fragments arranged by size:
4154 1261 663 593 237 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 364 1570 3785 364
Size: 1206 2215 3637
Fragments arranged by size:
3637 2215 1206
BseYI C'CCAG_C
Cuts at: 2114 2739 4483 5241 5818 6314 2114
Size: 625 1744 758 577 496 2858
Fragments arranged by size:
2858 1744 758 625 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2172 3451 3467 2172
Size: 1279 16 5763
Fragments arranged by size:
5763 1279 16
BsiEI CG_ry'CG
Cuts at: 104 4600 4646 5854 104
Size: 4496 46 1208 1308
Fragments arranged by size:
4496 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 488 1129 4072 4850 5040 5832 488
Size: 641 2943 778 190 792 1714
Fragments arranged by size:
2943 1714 792 778 641 190
BsmI GAATG_Cn'
Cuts at: 3323 3502 3323
Size: 179 6879
Bsp1286I G_dGCh'C
Cuts at: 123 488 1129 1227 4072 4683 4776 4850 5040
Size: 365 641 98 2845 611 93 74 190
Cuts at: 5040 5102 5832 123
Size: 62 730 1349
Fragments arranged by size:
2845 1349 730 641 611 365 190 98
93 74 62
BspEI T'CCGG_A
Cuts at: 1905 6278 1905
Size: 4373 2685
BspHI T'CATG_A
Cuts at: 3486 6234 3486
Size: 2748 4310
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1279 4108 4322 4624 5005 1279
Size: 2829 214 302 381 3332
Fragments arranged by size:
3332 2829 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5350 5404 6888 7053 50
Size: 5300 54 1484 165 55
Fragments arranged by size:
5300 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4970 4970
Size: 7058
BsrFI r'CCGG_y
Cuts at: 5056 5237 5446 5056
Size: 181 209 6668
Fragments arranged by size:
6668 209 181
BsrGI T'GTAC_A
Cuts at: 2667 2667
Size: 7058
BssSI C'ACGA_G
Cuts at: 5329 5687 5329
Size: 358 6700
BstAPI GCAn_nnn'nTGC
Cuts at: 798 1320 4435 798
Size: 522 3115 3421
Fragments arranged by size:
3421 3115 522
BstBI TT'CG_AA
Cuts at: 1563 2100 3339 5419 1563
Size: 537 1239 2080 3202
Fragments arranged by size:
3202 2080 1239 537
BstEII G'GTnAC_C
Cuts at: 832 832
Size: 7058
BstXI CCAn_nnnn'nTGG
Cuts at: 2017 2122 3888 2017
Size: 105 1766 5187
Fragments arranged by size:
5187 1766 105
BstYI r'GATC_y
Cuts at: 1 591 1464 2199 3147 3546 4083 4550 4573
Size: 590 873 735 948 399 537 467 23
Cuts at: 4573 4908 5154 6166 6252 6264 6610 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 948 873 735 590 537 467 449
399 346 335 246 86 23 12
BtgI C'CryG_G
Cuts at: 107 218 1055 1211 107
Size: 111 837 156 5954
Fragments arranged by size:
5954 837 156 111
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3978 5008 5189 5352 3978
Size: 1030 181 163 5684
Fragments arranged by size:
5684 1030 181 163
BtsI GCAGTG_nn'
Cuts at: 414 675 3455 3680 4301 414
Size: 261 2780 225 621 3171
Fragments arranged by size:
3171 2780 621 261 225
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 200 235 200
Size: 35 7023
DraI TTT'AAA
Cuts at: 4057 4057
Size: 7058
DrdI GACnn_nn'nnGTC
Cuts at: 3407 4764 5622 3407
Size: 1357 858 4843
Fragments arranged by size:
4843 1357 858
EaeI y'GGCC_r
Cuts at: 4643 4817 5208 5235 6580 4643
Size: 174 391 27 1345 5121
Fragments arranged by size:
5121 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4643 4643
Size: 7058
EarI CTCTTCn'nnn_
Cuts at: 1325 1838 2524 5081 5291 1325
Size: 513 686 2557 210 3092
Fragments arranged by size:
3092 2557 686 513 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3568 5266 5576 5722 6868 3568
Size: 1698 310 146 1146 3758
Fragments arranged by size:
3758 1698 1146 310 146
EcoICRI GAG'CTC
Cuts at: 4070 4070
Size: 7058
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 347 440 830 997 1494 1504 1594 2398 2828
Size: 93 390 167 497 10 90 804 430
Cuts at: 2828 2987 3485 3571 3910 4883 5315 6062 6330
Size: 159 498 86 339 973 432 747 268
Cuts at: 6330 347
Size: 1075
Fragments arranged by size:
1075 973 804 747 498 497 432 430
390 339 268 167 159 93 90 86
10
EcoO109I rG'GnC_Cy
Cuts at: 681 1224 4197 681
Size: 543 2973 3542
Fragments arranged by size:
3542 2973 543
EcoRI G'AATT_C
Cuts at: 184 1543 1579 2824 4062 184
Size: 1359 36 1245 1238 3180
Fragments arranged by size:
3180 1359 1245 1238 36
EcoRV GAT'ATC
Cuts at: 1218 1797 1218
Size: 579 6479
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3732 5077 3732
Size: 1345 5713
FspI TGC'GCA
Cuts at: 4839 4839
Size: 7058
HaeII r_GCGC'y
Cuts at: 230 4492 4740 5762 6741 230
Size: 4262 248 1022 979 547
Fragments arranged by size:
4262 1022 979 547 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 432 464 2851 2883 4900 4930 4932 4962 432
Size: 32 2387 32 2017 30 2 30 2528
Fragments arranged by size:
2528 2387 2017 32 32 30 30 2
HincII GTy'rAC
Cuts at: 470 540 4097 470
Size: 70 3557 3431
Fragments arranged by size:
3557 3431 70
HindIII A'AGCT_T
Cuts at: 1412 3709 1412
Size: 2297 4761
HpaI GTT'AAC
Cuts at: 470 470
Size: 7058
KasI G'GCGC_C
Cuts at: 226 4488 4736 226
Size: 4262 248 2548
Fragments arranged by size:
4262 2548 248
KpnI G_GTAC'C
Cuts at: 1695 4078 1695
Size: 2383 4675
MfeI C'AATT_G
Cuts at: 2015 2671 3390 2015
Size: 656 719 5683
Fragments arranged by size:
5683 719 656
MluI A'CGCG_T
Cuts at: 6491 6491
Size: 7058
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2415 4024 5729 5913 2415
Size: 1609 1705 184 3560
Fragments arranged by size:
3560 1705 1609 184
MscI TGG'CCA
Cuts at: 4819 4819
Size: 7058
MslI CAynn'nnrTG
Cuts at: 1666 3301 3926 5047 5174 1666
Size: 1635 625 1121 127 3550
Fragments arranged by size:
3550 1635 1121 625 127
MspA1I CmG'CkG
Cuts at: 2220 3505 3610 4169 4483 4843 5856 6101 2220
Size: 1285 105 559 314 360 1013 245 3177
Fragments arranged by size:
3177 1285 1013 559 360 314 245 105
NaeI GCC'GGC
Cuts at: 5239 5239
Size: 7058
NarI GG'CG_CC
Cuts at: 227 4489 4737 227
Size: 4262 248 2548
Fragments arranged by size:
4262 2548 248
NcoI C'CATG_G
Cuts at: 107 1055 107
Size: 948 6110
NdeI CA'TA_TG
Cuts at: 1813 3054 1813
Size: 1241 5817
NgoMIV G'CCGG_C
Cuts at: 5237 5237
Size: 7058
NheI G'CTAG_C
Cuts at: 3804 3804
Size: 7058
NsiI A_TGCA'T
Cuts at: 1085 3754 1085
Size: 2669 4389
NspI r_CATG'y
Cuts at: 975 1083 1087 3011 4111 4611 5518 975
Size: 108 4 1924 1100 500 907 2515
Fragments arranged by size:
2515 1924 1100 907 500 108 4
PciI A'CATG_T
Cuts at: 3007 5514 3007
Size: 2507 4551
PflMI CCAn_nnn'nTGG
Cuts at: 3442 3442
Size: 7058
PfoI T'CCnGG_A
Cuts at: 511 1471 3533 511
Size: 960 2062 4036
Fragments arranged by size:
4036 2062 960
PmeI GTTT'AAAC
Cuts at: 4057 4057
Size: 7058
PmlI CAC'GTG
Cuts at: 3208 3208
Size: 7058
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6197 6229 6197
Size: 32 7026
PsiI TTA'TAA
Cuts at: 1822 1873 1892 3979 1822
Size: 51 19 2087 4901
Fragments arranged by size:
4901 2087 51 19
PspOMI G'GGCC_C
Cuts at: 1223 1223
Size: 7058
PstI C_TGCA'G
Cuts at: 199 1920 2197 2885 4105 4790 199
Size: 1721 277 688 1220 685 2467
Fragments arranged by size:
2467 1721 1220 688 685 277
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7058
PvuII CAG'CTG
Cuts at: 2220 3505 4483 4843 2220
Size: 1285 978 360 4435
Fragments arranged by size:
4435 1285 978 360
RsrII CG'GwC_CG
Cuts at: 5253 5253
Size: 7058
SacI G_AGCT'C
Cuts at: 4072 4072
Size: 7058
SalI G'TCGA_C
Cuts at: 4095 4095
Size: 7058
SapI GCTCTTCn'nnn_
Cuts at: 5081 5291 5081
Size: 210 6848
SbfI CC_TGCA'GG
Cuts at: 4105 4105
Size: 7058
ScaI AGT'ACT
Cuts at: 2178 2405 6575 2178
Size: 227 4170 2661
Fragments arranged by size:
4170 2661 227
SexAI A'CCwGG_T
Cuts at: 747 747
Size: 7058
SfcI C'TryA_G
Cuts at: 33 195 653 1143 1897 1916 2193 2881 3142
Size: 162 458 490 754 19 277 688 261
Cuts at: 3142 3854 4101 4786 5779 5970 33
Size: 712 247 685 993 191 1121
Fragments arranged by size:
1121 993 754 712 688 685 490 458
277 261 247 191 162 19
SfoI GGC'GCC
Cuts at: 228 4490 4738 228
Size: 4262 248 2548
Fragments arranged by size:
4262 2548 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7058
SmaI CCC'GGG
Cuts at: 4080 4080
Size: 7058
SmlI C'TyrA_G
Cuts at: 724 3355 4213 5128 5620 5882 724
Size: 2631 858 915 492 262 1900
Fragments arranged by size:
2631 1900 915 858 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7058
SphI G_CATG'C
Cuts at: 1087 4111 4611 1087
Size: 3024 500 3534
Fragments arranged by size:
3534 3024 500
SspI AAT'ATT
Cuts at: 560 2097 560
Size: 1537 5521
StyI C'CwwG_G
Cuts at: 107 1055 3566 4049 4192 4546 107
Size: 948 2511 483 143 354 2619
Fragments arranged by size:
2619 2511 948 483 354 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 4641 4774 5233 5442 4641
Size: 133 459 209 6257
Fragments arranged by size:
6257 459 209 133
TatI w'GTAC_w
Cuts at: 2176 2403 2667 6573 2176
Size: 227 264 3906 2661
Fragments arranged by size:
3906 2661 264 227
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 937 969 2228 2260 3544 3576 6575 6607 937
Size: 32 1259 32 1284 32 2999 32 1388
Fragments arranged by size:
2999 1388 1284 1259 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4855 4855
Size: 7058
XbaI T'CTAG_A
Cuts at: 2637 4089 2637
Size: 1452 5606
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3419 4553 3419
Size: 1134 5924
XmaI C'CCGG_G
Cuts at: 4078 4078
Size: 7058
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NsiI NspI PciI PflMI PfoI PmeI PmlI PpiI
PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AscI AvrII BaeI BbvCI
BcgI BmgBI Bpu10I BsiWI BsmBI BssHII BstZ17I Bsu36I
ClaI DraIII EcoNI FseI FspAI NotI NruI PacI
PasI PpuMI PshAI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI XmnI ZraI