Restriction map for
pF1KA0209
(Circular) (Six-base) MAPSORT of: pF1KA0209.seq Check: 2629 from: 1 to: 8603
pF1KA0209 8603 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2472 2587 2472
Size: 115 8488
AccI GT'mk_AC
Cuts at: 4466 5641 4466
Size: 1175 7428
Acc65I G'GTAC_C
Cuts at: 3773 5619 3773
Size: 1846 6757
AclI AA'CG_TT
Cuts at: 8443 8443
Size: 8603
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 559 955 1990 2278 2338 2722 3529 4784
Size: 9 396 1035 288 60 384 807 1255
Cuts at: 4784 5287 5576 6428 6860 7213 7875 550
Size: 503 289 852 432 353 662 1278
Fragments arranged by size:
1278 1255 1035 852 807 662 503 432
396 384 353 289 288 60 9
AflII C'TTAA_G
Cuts at: 1027 1027
Size: 8603
AflIII A'CryG_T
Cuts at: 226 3056 4565 5955 7755 8036 226
Size: 2830 1509 1390 1800 281 793
Fragments arranged by size:
2830 1800 1509 1390 793 281
AhdI GACnn_n'nnGTC
Cuts at: 5583 5583
Size: 8603
AleI CACnn'nnGTG
Cuts at: 1324 1921 1324
Size: 597 8006
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1065 1501 2237 1065
Size: 436 736 7431
Fragments arranged by size:
7431 736 436
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2320 2352 2320
Size: 32 8571
AlwNI CAG_nnn'CTG
Cuts at: 1062 2745 2853 3564 3826 4071 4387 5908 7346
Size: 1683 108 711 262 245 316 1521 1438
Cuts at: 7346 1062
Size: 2319
Fragments arranged by size:
2319 1683 1521 1438 711 316 262 245
108
ApaLI G'TGCA_C
Cuts at: 1050 4561 7441 1050
Size: 3511 2880 2212
Fragments arranged by size:
3511 2880 2212
ApoI r'AATT_y
Cuts at: 359 983 2004 2170 3732 4087 5061 5607 7956
Size: 624 1021 166 1562 355 974 546 2349
Cuts at: 7956 8212 359
Size: 256 750
Fragments arranged by size:
2349 1562 1021 974 750 624 546 355
256 166
AseI AT'TA_AT
Cuts at: 20 20
Size: 8603
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8603
AvaI C'yCGr_G
Cuts at: 1895 4287 5163 5623 1895
Size: 2392 876 460 4875
Fragments arranged by size:
4875 2392 876 460
BamHI G'GATC_C
Cuts at: 1619 4538 5628 1619
Size: 2919 1090 4594
Fragments arranged by size:
4594 2919 1090
BanI G'GyrC_C
Cuts at: 3773 5619 6033 6281 6316 3773
Size: 1846 414 248 35 6060
Fragments arranged by size:
6060 1846 414 248 35
BanII G_rGCy'C
Cuts at: 180 827 1864 3416 5617 6647 180
Size: 647 1037 1552 2201 1030 2136
Fragments arranged by size:
2201 2136 1552 1037 1030 647
BbeI G_GCGC'C
Cuts at: 6037 6285 6037
Size: 248 8355
BbsI GAAGACnn'nnnn_
Cuts at: 617 936 1613 2266 3096 5500 617
Size: 319 677 653 830 2404 3720
Fragments arranged by size:
3720 2404 830 677 653 319
BbvCI CC'TCA_GC
Cuts at: 5455 5455
Size: 8603
BciVI GTATCCnnnnn_n'
Cuts at: 241 1026 2052 2265 4138 6501 7557 8603 241
Size: 785 1026 213 1873 2363 1056 1046 241
Fragments arranged by size:
2363 1873 1056 1046 1026 785 241 213
BclI T'GATC_A
Cuts at: 6123 6123
Size: 8603
BglI GCCn_nnn'nGGC
Cuts at: 1199 1199
Size: 8603
BglII A'GATC_T
Cuts at: 4040 6118 8155 4040
Size: 2078 2037 4488
Fragments arranged by size:
4488 2078 2037
BlpI GC'TnA_GC
Cuts at: 821 1852 3410 4060 5715 821
Size: 1031 1558 650 1655 3709
Fragments arranged by size:
3709 1655 1558 1031 650
Bme1580I G_kGCm'C
Cuts at: 1054 4565 6228 6321 7445 1054
Size: 3511 1663 93 1124 2212
Fragments arranged by size:
3511 2212 1663 1124 93
BmgBI CAC'GTC
Cuts at: 227 554 227
Size: 327 8276
BmrI ACTGGGnnnn_n'
Cuts at: 4242 5815 5896 6231 8375 4242
Size: 1573 81 335 2144 4470
Fragments arranged by size:
4470 2144 1573 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1554 3264 4524 6970 1554
Size: 1710 1260 2446 3187
Fragments arranged by size:
3187 2446 1710 1260
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 1848 2038 2215 2262 2584 2734 2767 3086
Size: 1412 190 177 47 322 150 33 319
Cuts at: 3086 3259 3586 3673 4612 436
Size: 173 327 87 939 4427
Fragments arranged by size:
4427 1412 939 327 322 319 190 177
173 150 87 47 33
Bpu10I CC'TnA_GC
Cuts at: 2794 5455 2794
Size: 2661 5942
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 1747 3844 3919 4798 5320 5779 6658 7372
Size: 1143 2097 75 879 522 459 879 714
Cuts at: 7372 7670 604
Size: 298 1537
Fragments arranged by size:
2097 1537 1143 879 879 714 522 459
298 75
BsaI GGTCTCn'nnnn_
Cuts at: 2971 3013 3946 4660 8307 2971
Size: 42 933 714 3647 3267
Fragments arranged by size:
3647 3267 933 714 42
BsaAI yAC'GTr
Cuts at: 4422 5956 4422
Size: 1534 7069
BsaBI GATnn'nnATC
Cuts at: 6 4039 4358 4861 5038 6117 8154 6
Size: 4033 319 503 177 1079 2037 455
Fragments arranged by size:
4033 2037 1079 503 455 319 177
BsaHI Gr'CG_yC
Cuts at: 5131 5341 6034 6282 6984 8480 5131
Size: 210 693 248 702 1496 5254
Fragments arranged by size:
5254 1496 702 693 248 210
BsaWI w'CCGG_w
Cuts at: 2165 2867 3416 4907 5792 6313 6991 7402 7549
Size: 702 549 1491 885 521 678 411 147
Cuts at: 7549 7823 2165
Size: 274 2945
Fragments arranged by size:
2945 1491 885 702 678 549 521 411
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 475 505 2024 2054 2740 2770 3748 3778 4283
Size: 30 1519 30 686 30 978 30 505
Cuts at: 4283 4313 4672 4702 4939 4969 475
Size: 30 359 30 237 30 4109
Fragments arranged by size:
4109 1519 978 686 505 359 237 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 744 1080 3772 744
Size: 336 2692 5575
Fragments arranged by size:
5575 2692 336
BseYI C'CCAG_C
Cuts at: 182 494 1197 1338 1959 4852 5080 5391 6028
Size: 312 703 141 621 2893 228 311 637
Cuts at: 6028 6786 7451 7859 182
Size: 758 665 408 926
Fragments arranged by size:
2893 926 758 703 665 637 621 408
312 311 228 141
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 478 822 3699 478
Size: 344 2877 5382
Fragments arranged by size:
5382 2877 344
BsiEI CG_ry'CG
Cuts at: 104 2356 6145 6191 7421 104
Size: 2252 3789 46 1230 1286
Fragments arranged by size:
3789 2252 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 827 1054 1864 1930 3416 3599 4565 5173 5617
Size: 227 810 66 1486 183 966 608 444
Cuts at: 5617 6395 6585 7445 827
Size: 778 190 860 1985
Fragments arranged by size:
1985 1486 966 860 810 778 608 444
227 190 183 66
BsmI GAATG_Cn'
Cuts at: 2094 2668 4311 4602 2094
Size: 574 1643 291 6095
Fragments arranged by size:
6095 1643 574 291
BsmBI CGTCTCn'nnnn_
Cuts at: 217 2412 3288 3934 217
Size: 2195 876 646 4886
Fragments arranged by size:
4886 2195 876 646
Bsp1286I G_dGCh'C
Cuts at: 180 827 1054 1864 1930 3416 3599 4565 5173
Size: 647 227 810 66 1486 183 966 608
Cuts at: 5173 5617 6228 6321 6395 6585 6647 7445 180
Size: 444 611 93 74 190 62 798 1338
Fragments arranged by size:
1486 1338 966 810 798 647 611 608
444 227 190 183 93 74 66 62
BspEI T'CCGG_A
Cuts at: 3416 3416
Size: 8603
BspHI T'CATG_A
Cuts at: 2084 4103 5294 7035 2084
Size: 2019 1191 1741 3652
Fragments arranged by size:
3652 2019 1741 1191
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2472 2587 3734 5653 5867 6169 6550 2472
Size: 115 1147 1919 214 302 381 4525
Fragments arranged by size:
4525 1919 1147 381 302 214 115
BsrBI CCG'CTC
Cuts at: 50 138 6895 6949 8433 8598 50
Size: 88 6757 54 1484 165 55
Fragments arranged by size:
6757 1484 165 88 55 54
BsrDI GCAATG_nn'
Cuts at: 743 2156 2299 3605 6515 743
Size: 1413 143 1306 2910 2831
Fragments arranged by size:
2910 2831 1413 1306 143
BsrFI r'CCGG_y
Cuts at: 3926 6601 6782 3926
Size: 2675 181 5747
Fragments arranged by size:
5747 2675 181
BsrGI T'GTAC_A
Cuts at: 3764 3779 3764
Size: 15 8588
BssSI C'ACGA_G
Cuts at: 3520 6874 7582 3520
Size: 3354 708 4541
Fragments arranged by size:
4541 3354 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3555 3713 5980 3555
Size: 158 2267 6178
Fragments arranged by size:
6178 2267 158
BstBI TT'CG_AA
Cuts at: 6964 6964
Size: 8603
BstEII G'GTnAC_C
Cuts at: 1248 2950 1248
Size: 1702 6901
BstXI CCAn_nnnn'nTGG
Cuts at: 1326 1338 3087 3618 5595 1326
Size: 12 1749 531 1977 4334
Fragments arranged by size:
4334 1977 1749 531 12
BstYI r'GATC_y
Cuts at: 1 749 951 1023 1619 4040 4538 4631 4835
Size: 748 202 72 596 2421 498 93 204
Cuts at: 4835 4862 5570 5628 6095 6118 6453 6699 7005
Size: 27 708 58 467 23 335 246 306
Cuts at: 7005 7017 7103 8155 1
Size: 12 86 1052 449
Fragments arranged by size:
2421 1052 748 708 596 498 467 449
335 306 246 204 202 93 86 72
58 27 23 12
Bsu36I CC'TnA_GG
Cuts at: 908 3404 4114 4787 5180 908
Size: 2496 710 673 393 4331
Fragments arranged by size:
4331 2496 710 673 393
BtgI C'CryG_G
Cuts at: 107 1322 2882 4197 4616 5588 107
Size: 1215 1560 1315 419 972 3122
Fragments arranged by size:
3122 1560 1315 1215 972 419
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 218 900 6553 6734 6897 218
Size: 682 5653 181 163 1924
Fragments arranged by size:
5653 1924 682 181 163
BtsI GCAGTG_nn'
Cuts at: 1875 3212 3883 5846 1875
Size: 1337 671 1963 4632
Fragments arranged by size:
4632 1963 1337 671
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1484 1519 1484
Size: 35 8568
DraI TTT'AAA
Cuts at: 2652 5602 2652
Size: 2950 5653
DraIII CAC_nnn'GTG
Cuts at: 147 1308 1830 2916 147
Size: 1161 522 1086 5834
Fragments arranged by size:
5834 1161 1086 522
DrdI GACnn_nn'nnGTC
Cuts at: 1756 2122 4720 6309 7653 1756
Size: 366 2598 1589 1344 2706
Fragments arranged by size:
2706 2598 1589 1344 366
EaeI y'GGCC_r
Cuts at: 149 428 1328 3077 4152 4619 5324 5540 6188
Size: 279 900 1749 1075 467 705 216 648
Cuts at: 6188 6362 6753 6780 8125 149
Size: 174 391 27 1345 627
Fragments arranged by size:
1749 1345 1075 900 705 648 627 467
391 279 216 174 27
EagI C'GGCC_G
Cuts at: 6188 6188
Size: 8603
EarI CTCTTCn'nnn_
Cuts at: 832 2080 3986 4212 4760 5200 6626 6836 832
Size: 1248 1906 226 548 440 1426 210 2599
Fragments arranged by size:
2599 1906 1426 1248 548 440 226 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4036 6811 7553 7699 8413 4036
Size: 2775 742 146 714 4226
Fragments arranged by size:
4226 2775 742 714 146
EcoICRI GAG'CTC
Cuts at: 825 1862 3414 5615 825
Size: 1037 1552 2201 3813
Fragments arranged by size:
3813 2201 1552 1037
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 550 559 955 1848 1990 2038 2215 2262
Size: 114 9 396 893 142 48 177 47
Cuts at: 2262 2278 2338 2584 2722 2734 2767 3086 3259
Size: 16 60 246 138 12 33 319 173
Cuts at: 3259 3529 3586 3673 4612 4784 5287 5576 6428
Size: 270 57 87 939 172 503 289 852
Cuts at: 6428 6860 7213 7875 436
Size: 432 353 662 1164
Fragments arranged by size:
1164 939 893 852 662 503 432 396
353 319 289 270 246 177 173 172
142 138 114 87 60 57 48 47
33 16 12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2479 5179 2479
Size: 2700 5903
EcoO109I rG'GnC_Cy
Cuts at: 491 1070 2742 3039 3376 4818 5742 491
Size: 579 1672 297 337 1442 924 3352
Fragments arranged by size:
3352 1672 1442 924 579 337 297
EcoRI G'AATT_C
Cuts at: 5061 5607 5061
Size: 546 8057
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 960 1161 2283 4773 6622 960
Size: 201 1122 2490 1849 2941
Fragments arranged by size:
2941 2490 1849 1122 201
FspI TGC'GCA
Cuts at: 4453 6384 4453
Size: 1931 6672
HaeII r_GCGC'y
Cuts at: 5404 6037 6285 7515 8286 5404
Size: 633 248 1230 771 5721
Fragments arranged by size:
5721 1230 771 633 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 459 475 491 507 1608 1640 1891 1923 2439
Size: 16 16 16 1101 32 251 32 516
Cuts at: 2439 2471 5569 5601 6445 6475 6477 6507 459
Size: 32 3098 32 844 30 2 30 2555
Fragments arranged by size:
3098 2555 1101 844 516 251 32 32
32 32 30 30 16 16 16 2
HincII GTy'rAC
Cuts at: 5642 5642
Size: 8603
HindIII A'AGCT_T
Cuts at: 3334 3334
Size: 8603
KasI G'GCGC_C
Cuts at: 6033 6281 6033
Size: 248 8355
KpnI G_GTAC'C
Cuts at: 3777 5623 3777
Size: 1846 6757
MluI A'CGCG_T
Cuts at: 8036 8036
Size: 8603
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2232 3848 4122 4439 5240 5408 5496 7362 7546
Size: 1616 274 317 801 168 88 1866 184
Cuts at: 7546 2232
Size: 3289
Fragments arranged by size:
3289 1866 1616 801 317 274 184 168
88
MscI TGG'CCA
Cuts at: 151 430 1330 3079 5542 6364 151
Size: 279 900 1749 2463 822 2390
Fragments arranged by size:
2463 2390 1749 900 822 279
MslI CAynn'nnrTG
Cuts at: 665 1258 1324 1459 1921 2086 2219 2881 3085
Size: 593 66 135 462 165 133 662 204
Cuts at: 3085 6592 6719 665
Size: 3507 127 2549
Fragments arranged by size:
3507 2549 662 593 462 204 165 135
133 127 66
MspA1I CmG'CkG
Cuts at: 173 1171 2850 3180 3237 3561 4199 4239 5251
Size: 998 1679 330 57 324 638 40 1012
Cuts at: 5251 5714 6028 6388 7172 7417 173
Size: 463 314 360 784 245 1359
Fragments arranged by size:
1679 1359 1012 998 784 638 463 360
330 324 314 245 57 40
NaeI GCC'GGC
Cuts at: 6784 6784
Size: 8603
NarI GG'CG_CC
Cuts at: 6034 6282 6034
Size: 248 8355
NcoI C'CATG_G
Cuts at: 107 2882 107
Size: 2775 5828
NgoMIV G'CCGG_C
Cuts at: 6782 6782
Size: 8603
NspI r_CATG'y
Cuts at: 1614 2733 2924 3558 4569 5323 5656 6156 7759
Size: 1119 191 634 1011 754 333 500 1603
Cuts at: 7759 1614
Size: 2458
Fragments arranged by size:
2458 1603 1119 1011 754 634 500 333
191
PasI CC'CwG_GG
Cuts at: 5422 5422
Size: 8603
PciI A'CATG_T
Cuts at: 4565 7755 4565
Size: 3190 5413
PflMI CCAn_nnn'nTGG
Cuts at: 2745 2745
Size: 8603
PfoI T'CCnGG_A
Cuts at: 745 2054 2744 3650 5278 745
Size: 1309 690 906 1628 4070
Fragments arranged by size:
4070 1628 1309 906 690
PmeI GTTT'AAAC
Cuts at: 5602 5602
Size: 8603
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4264 4296 7049 7081 4264
Size: 32 2753 32 5786
Fragments arranged by size:
5786 2753 32 32
PpuMI rG'GwC_Cy
Cuts at: 491 2742 3039 3376 4818 491
Size: 2251 297 337 1442 4276
Fragments arranged by size:
4276 2251 1442 337 297
PshAI GACnn'nnGTC
Cuts at: 1647 5034 1647
Size: 3387 5216
PsiI TTA'TAA
Cuts at: 4399 4399
Size: 8603
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1369 1401 1369
Size: 32 8571
PstI C_TGCA'G
Cuts at: 197 357 3236 3594 5074 5256 5650 6335 197
Size: 160 2879 358 1480 182 394 685 2465
Fragments arranged by size:
2879 2465 1480 685 394 358 182 160
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8603
PvuII CAG'CTG
Cuts at: 1171 2850 3180 3237 3561 4239 5251 6028 6388
Size: 1679 330 57 324 678 1012 777 360
Cuts at: 6388 1171
Size: 3386
Fragments arranged by size:
3386 1679 1012 777 678 360 330 324
57
RsrII CG'GwC_CG
Cuts at: 6798 6798
Size: 8603
SacI G_AGCT'C
Cuts at: 827 1864 3416 5617 827
Size: 1037 1552 2201 3813
Fragments arranged by size:
3813 2201 1552 1037
SacII CC_GC'GG
Cuts at: 4200 4200
Size: 8603
SalI G'TCGA_C
Cuts at: 5640 5640
Size: 8603
SapI GCTCTTCn'nnn_
Cuts at: 832 6626 6836 832
Size: 5794 210 2599
Fragments arranged by size:
5794 2599 210
SbfI CC_TGCA'GG
Cuts at: 5650 5650
Size: 8603
ScaI AGT'ACT
Cuts at: 2758 8120 2758
Size: 5362 3241
SexAI A'CCwGG_T
Cuts at: 1304 2738 3635 5594 1304
Size: 1434 897 1959 4313
Fragments arranged by size:
4313 1959 1434 897
SfcI C'TryA_G
Cuts at: 33 193 353 1996 3232 3590 5070 5252 5646
Size: 160 160 1643 1236 358 1480 182 394
Cuts at: 5646 6331 7299 7490 33
Size: 685 968 191 1146
Fragments arranged by size:
1643 1480 1236 1146 968 685 394 358
191 182 160 160
SfoI GGC'GCC
Cuts at: 6035 6283 6035
Size: 248 8355
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8603
SmaI CCC'GGG
Cuts at: 1897 4289 5165 5625 1897
Size: 2392 876 460 4875
Fragments arranged by size:
4875 2392 876 460
SmlI C'TyrA_G
Cuts at: 583 1027 1762 3859 3934 4813 5335 5758 6673
Size: 444 735 2097 75 879 522 423 915
Cuts at: 6673 7387 7649 583
Size: 714 262 1537
Fragments arranged by size:
2097 1537 915 879 735 714 522 444
423 262 75
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8603
SphI G_CATG'C
Cuts at: 5323 5656 6156 5323
Size: 333 500 7770
Fragments arranged by size:
7770 500 333
StuI AGG'CCT
Cuts at: 1241 3647 4753 1241
Size: 2406 1106 5091
Fragments arranged by size:
5091 2406 1106
StyI C'CwwG_G
Cuts at: 107 165 518 1217 2228 2882 5737 6091 107
Size: 58 353 699 1011 654 2855 354 2619
Fragments arranged by size:
2855 2619 1011 699 654 354 353 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 781 1236 1258 1637 2342 2938 3235 3614 5139
Size: 455 22 379 705 596 297 379 1525
Cuts at: 5139 6186 6319 6778 6987 781
Size: 1047 133 459 209 2397
Fragments arranged by size:
2397 1525 1047 705 596 459 455 379
379 297 209 133 22
TatI w'GTAC_w
Cuts at: 1418 1547 2613 2756 3551 3764 3779 8118 1418
Size: 129 1066 143 795 213 15 4339 1903
Fragments arranged by size:
4339 1903 1066 795 213 143 129 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2024 2056 3041 3073 3862 3894 4283 4315 4569
Size: 32 985 32 789 32 389 32 254
Cuts at: 4569 4601 8120 8152 2024
Size: 32 3519 32 2475
Fragments arranged by size:
3519 2475 985 789 389 254 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 6400 6400
Size: 8603
XbaI T'CTAG_A
Cuts at: 5634 5634
Size: 8603
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1260 1917 5046 5394 5479 6098 1260
Size: 657 3129 348 85 619 3765
Fragments arranged by size:
3765 3129 657 619 348 85
XmaI C'CCGG_G
Cuts at: 1895 4287 5163 5623 1895
Size: 2392 876 460 4875
Fragments arranged by size:
4875 2392 876 460
XmnI GAAnn'nnTTC
Cuts at: 1180 4753 1180
Size: 3573 5030
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AgeI ApaI AscI AvrII BaeI BcgI
BfrBI BmtI BsiWI BssHII BstZ17I ClaI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PspOMI PspXI SanDI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI