Restriction map for
pF1KA0227
(Circular) (Six-base) MAPSORT of: pF1KA0227.seq Check: 9044 from: 1 to: 3779
pF1KA0227 3779 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AccI GT'mk_AC
Cuts at: 817 817
Size: 3779
Acc65I G'GTAC_C
Cuts at: 795 795
Size: 3779
AclI AA'CG_TT
Cuts at: 3619 3619
Size: 3779
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 457 1604 2036 2783 3051 388
Size: 69 1147 432 747 268 1116
Fragments arranged by size:
1147 1116 747 432 268 69
AflIII A'CryG_T
Cuts at: 1131 2235 3212 1131
Size: 1104 977 1698
Fragments arranged by size:
1698 1104 977
AgeI A'CCGG_T
Cuts at: 611 2167 611
Size: 1556 2223
AleI CACnn'nnGTG
Cuts at: 703 703
Size: 3779
AlwNI CAG_nnn'CTG
Cuts at: 1084 2651 1084
Size: 1567 2212
ApaI G_GGCC'C
Cuts at: 435 435
Size: 3779
ApaLI G'TGCA_C
Cuts at: 2549 2549
Size: 3779
ApoI r'AATT_y
Cuts at: 322 783 3132 3388 322
Size: 461 2349 256 713
Fragments arranged by size:
2349 713 461 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3779
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3779
AvaI C'yCGr_G
Cuts at: 147 201 388 799 147
Size: 54 187 411 3127
Fragments arranged by size:
3127 411 187 54
BamHI G'GATC_C
Cuts at: 804 804
Size: 3779
BanI G'GyrC_C
Cuts at: 795 1209 1457 1492 795
Size: 414 248 35 3082
Fragments arranged by size:
3082 414 248 35
BanII G_rGCy'C
Cuts at: 124 154 222 266 435 793 1823 124
Size: 30 68 44 169 358 1030 2080
Fragments arranged by size:
2080 1030 358 169 68 44 30
BbeI G_GCGC'C
Cuts at: 1213 1461 1213
Size: 248 3531
BciVI GTATCCnnnnn_n'
Cuts at: 1677 2438 3779 1677
Size: 761 1341 1677
Fragments arranged by size:
1677 1341 761
BclI T'GATC_A
Cuts at: 1299 1299
Size: 3779
BglI GCCn_nnn'nGGC
Cuts at: 256 424 256
Size: 168 3611
BglII A'GATC_T
Cuts at: 1294 3331 1294
Size: 2037 1742
BlpI GC'TnA_GC
Cuts at: 530 891 530
Size: 361 3418
Bme1580I G_kGCm'C
Cuts at: 435 1404 1497 2553 435
Size: 969 93 1056 1661
Fragments arranged by size:
1661 1056 969 93
BmrI ACTGGGnnnn_n'
Cuts at: 991 1072 1407 3551 991
Size: 81 335 2144 1219
Fragments arranged by size:
2144 1219 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 423 2146 423
Size: 1723 2056
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 508 382
Size: 126 3653
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 955 1834 2326 2624 955
Size: 879 492 298 2110
Fragments arranged by size:
2110 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3483 3483
Size: 3779
BsaAI yAC'GTr
Cuts at: 1132 1132
Size: 3779
BsaBI GATnn'nnATC
Cuts at: 6 1293 3330 6
Size: 1287 2037 455
Fragments arranged by size:
2037 1287 455
BsaHI Gr'CG_yC
Cuts at: 1210 1458 2160 3656 1210
Size: 248 702 1496 1333
Fragments arranged by size:
1496 1333 702 248
BsaWI w'CCGG_w
Cuts at: 611 968 1489 2167 2441 2588 2999 611
Size: 357 521 678 274 147 411 1391
Fragments arranged by size:
1391 678 521 411 357 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 230 472 230
Size: 242 3537
BseYI C'CCAG_C
Cuts at: 222 427 1204 1962 2539 3035 222
Size: 205 777 758 577 496 966
Fragments arranged by size:
966 777 758 577 496 205
BsiEI CG_ry'CG
Cuts at: 104 131 164 251 260 447 1321 1367 2575
Size: 27 33 87 9 187 874 46 1208
Cuts at: 2575 104
Size: 1308
Fragments arranged by size:
1308 1208 874 187 87 46 33 27
9
BsiHKAI G_wGCw'C
Cuts at: 266 793 1571 1761 2553 266
Size: 527 778 190 792 1492
Fragments arranged by size:
1492 792 778 527 190
Bsp1286I G_dGCh'C
Cuts at: 124 154 222 266 435 793 1404 1497 1571
Size: 30 68 44 169 358 611 93 74
Cuts at: 1571 1761 1823 2553 124
Size: 190 62 730 1350
Fragments arranged by size:
1350 730 611 358 190 169 93 74
68 62 44 30
BspEI T'CCGG_A
Cuts at: 2999 2999
Size: 3779
BspHI T'CATG_A
Cuts at: 2955 2955
Size: 3779
BspMI ACCTGCnnnn'nnnn_
Cuts at: 829 1043 1345 1726 829
Size: 214 302 381 2882
Fragments arranged by size:
2882 381 302 214
BsrBI CCG'CTC
Cuts at: 50 402 2071 2125 3609 3774 50
Size: 352 1669 54 1484 165 55
Fragments arranged by size:
1669 1484 352 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1691 1691
Size: 3779
BsrFI r'CCGG_y
Cuts at: 255 611 1777 1958 2167 255
Size: 356 1166 181 209 1867
Fragments arranged by size:
1867 1166 356 209 181
BssHII G'CGCG_C
Cuts at: 411 411
Size: 3779
BssSI C'ACGA_G
Cuts at: 280 2050 2408 280
Size: 1770 358 1651
Fragments arranged by size:
1770 1651 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1156 1156
Size: 3779
BstBI TT'CG_AA
Cuts at: 2140 2140
Size: 3779
BstYI r'GATC_y
Cuts at: 1 804 1271 1294 1629 1875 2887 2973 2985
Size: 803 467 23 335 246 1012 86 12
Cuts at: 2985 3331 1
Size: 346 449
Fragments arranged by size:
1012 803 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 158 770 107
Size: 51 612 3116
Fragments arranged by size:
3116 612 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1729 1910 2073 1729
Size: 181 163 3435
Fragments arranged by size:
3435 181 163
BtsI GCAGTG_nn'
Cuts at: 310 1022 310
Size: 712 3067
DraI TTT'AAA
Cuts at: 778 778
Size: 3779
DrdI GACnn_nn'nnGTC
Cuts at: 1485 2343 1485
Size: 858 2921
EaeI y'GGCC_r
Cuts at: 128 161 178 248 257 444 1364 1538 1929
Size: 33 17 70 9 187 920 174 391
Cuts at: 1929 1956 3301 128
Size: 27 1345 606
Fragments arranged by size:
1345 920 606 391 187 174 70 33
27 17 9
EagI C'GGCC_G
Cuts at: 128 161 248 257 444 1364 128
Size: 33 87 9 187 920 2543
Fragments arranged by size:
2543 920 187 87 33 9
EarI CTCTTCn'nnn_
Cuts at: 1802 2012 1802
Size: 210 3569
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 227 1987 2297 2443 3589 227
Size: 1760 310 146 1146 417
Fragments arranged by size:
1760 1146 417 310 146
EcoICRI GAG'CTC
Cuts at: 264 791 264
Size: 527 3252
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 388 457 508 1604 2036 2783 3051 382
Size: 6 69 51 1096 432 747 268 1110
Fragments arranged by size:
1110 1096 747 432 268 69 51 6
EcoNI CCTnn'n_nnAGG
Cuts at: 454 523 454
Size: 69 3710
EcoO109I rG'GnC_Cy
Cuts at: 431 432 603 680 918 431
Size: 1 171 77 238 3292
Fragments arranged by size:
3292 238 171 77 1
EcoRI G'AATT_C
Cuts at: 783 783
Size: 3779
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1798 1798
Size: 3779
FspI TGC'GCA
Cuts at: 1560 1560
Size: 3779
HaeII r_GCGC'y
Cuts at: 1213 1461 2483 3462 1213
Size: 248 1022 979 1530
Fragments arranged by size:
1530 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1621 1651 1653 1683 1621
Size: 30 2 30 3717
Fragments arranged by size:
3717 30 30 2
HincII GTy'rAC
Cuts at: 818 818
Size: 3779
KasI G'GCGC_C
Cuts at: 1209 1457 1209
Size: 248 3531
KpnI G_GTAC'C
Cuts at: 799 799
Size: 3779
MluI A'CGCG_T
Cuts at: 3212 3212
Size: 3779
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 294 2450 2634 294
Size: 2156 184 1439
Fragments arranged by size:
2156 1439 184
MscI TGG'CCA
Cuts at: 1540 1540
Size: 3779
MslI CAynn'nnrTG
Cuts at: 703 1768 1895 703
Size: 1065 127 2587
Fragments arranged by size:
2587 1065 127
MspA1I CmG'CkG
Cuts at: 160 177 192 723 890 1204 1564 2577 2822
Size: 17 15 531 167 314 360 1013 245
Cuts at: 2822 160
Size: 1117
Fragments arranged by size:
1117 1013 531 360 314 245 167 17
15
NaeI GCC'GGC
Cuts at: 257 1960 257
Size: 1703 2076
NarI GG'CG_CC
Cuts at: 1210 1458 1210
Size: 248 3531
NcoI C'CATG_G
Cuts at: 107 770 107
Size: 663 3116
NgoMIV G'CCGG_C
Cuts at: 255 1958 255
Size: 1703 2076
NspI r_CATG'y
Cuts at: 832 1332 2239 832
Size: 500 907 2372
Fragments arranged by size:
2372 907 500
PciI A'CATG_T
Cuts at: 2235 2235
Size: 3779
PmeI GTTT'AAAC
Cuts at: 778 778
Size: 3779
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2918 2950 2918
Size: 32 3747
PpuMI rG'GwC_Cy
Cuts at: 680 680
Size: 3779
PspOMI G'GGCC_C
Cuts at: 431 431
Size: 3779
PstI C_TGCA'G
Cuts at: 826 1511 826
Size: 685 3094
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3779
PvuII CAG'CTG
Cuts at: 723 1204 1564 723
Size: 481 360 2938
Fragments arranged by size:
2938 481 360
RsrII CG'GwC_CG
Cuts at: 1974 1974
Size: 3779
SacI G_AGCT'C
Cuts at: 266 793 266
Size: 527 3252
SacII CC_GC'GG
Cuts at: 161 161
Size: 3779
SalI G'TCGA_C
Cuts at: 816 816
Size: 3779
SapI GCTCTTCn'nnn_
Cuts at: 1802 2012 1802
Size: 210 3569
SbfI CC_TGCA'GG
Cuts at: 826 826
Size: 3779
ScaI AGT'ACT
Cuts at: 3296 3296
Size: 3779
SfcI C'TryA_G
Cuts at: 33 822 1507 2500 2691 33
Size: 789 685 993 191 1121
Fragments arranged by size:
1121 993 789 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 256 256
Size: 3779
SfoI GGC'GCC
Cuts at: 1211 1459 1211
Size: 248 3531
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3779
SmaI CCC'GGG
Cuts at: 203 390 801 203
Size: 187 411 3181
Fragments arranged by size:
3181 411 187
SmlI C'TyrA_G
Cuts at: 934 1849 2341 2603 934
Size: 915 492 262 2110
Fragments arranged by size:
2110 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3779
SphI G_CATG'C
Cuts at: 832 1332 832
Size: 500 3279
SspI AAT'ATT
Cuts at: 562 562
Size: 3779
StyI C'CwwG_G
Cuts at: 107 137 294 636 770 913 1267 107
Size: 30 157 342 134 143 354 2619
Fragments arranged by size:
2619 354 342 157 143 134 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1362 1495 1954 2163 1362
Size: 133 459 209 2978
Fragments arranged by size:
2978 459 209 133
TatI w'GTAC_w
Cuts at: 3294 3294
Size: 3779
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3296 3328 3296
Size: 32 3747
Tth111I GACn'n_nGTC
Cuts at: 1576 1576
Size: 3779
XbaI T'CTAG_A
Cuts at: 810 810
Size: 3779
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1274 1274
Size: 3779
XmaI C'CCGG_G
Cuts at: 201 388 799 201
Size: 187 411 3181
Fragments arranged by size:
3181 411 187
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI
PspXI PsrI SanDI SexAI SgrAI SnaBI SrfI StuI
SwaI XhoI XmnI ZraI