Restriction map for
pF1KA0229
(Circular) (Six-base) MAPSORT of: pF1KA0229.seq Check: 4222 from: 1 to: 6515
pF1KA0229 6515 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AatII G_ACGT'C
Cuts at: 926 2677 926
Size: 1751 4764
AccI GT'mk_AC
Cuts at: 3553 3553
Size: 6515
Acc65I G'GTAC_C
Cuts at: 3531 3531
Size: 6515
AclI AA'CG_TT
Cuts at: 3509 6355 3509
Size: 2846 3669
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1193 2569 2790 4340 4772 5519 5787 1193
Size: 1376 221 1550 432 747 268 1921
Fragments arranged by size:
1921 1550 1376 747 432 268 221
AfeI AGC'GCT
Cuts at: 2803 2803
Size: 6515
AflIII A'CryG_T
Cuts at: 3867 4971 5948 3867
Size: 1104 977 4434
Fragments arranged by size:
4434 1104 977
AgeI A'CCGG_T
Cuts at: 3372 4903 3372
Size: 1531 4984
AleI CACnn'nnGTG
Cuts at: 595 1883 595
Size: 1288 5227
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 383 874 884 383
Size: 491 10 6014
Fragments arranged by size:
6014 491 10
AlwNI CAG_nnn'CTG
Cuts at: 126 919 1141 1405 2319 3820 5387 126
Size: 793 222 264 914 1501 1567 1254
Fragments arranged by size:
1567 1501 1254 914 793 264 222
ApaLI G'TGCA_C
Cuts at: 5285 5285
Size: 6515
ApoI r'AATT_y
Cuts at: 649 3519 5868 6124 649
Size: 2870 2349 256 1040
Fragments arranged by size:
2870 2349 1040 256
AseI AT'TA_AT
Cuts at: 20 1009 20
Size: 989 5526
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6515
AvaI C'yCGr_G
Cuts at: 1448 2792 3386 3535 1448
Size: 1344 594 149 4428
Fragments arranged by size:
4428 1344 594 149
AvrII C'CTAG_G
Cuts at: 1315 1426 1315
Size: 111 6404
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3150 3183 3150
Size: 33 6482
BamHI G'GATC_C
Cuts at: 2810 3540 2810
Size: 730 5785
BanI G'GyrC_C
Cuts at: 149 1711 2651 2756 2784 3331 3375 3531 3945
Size: 1562 940 105 28 547 44 156 414
Cuts at: 3945 4193 4228 149
Size: 248 35 2436
Fragments arranged by size:
2436 1562 940 547 414 248 156 105
44 35 28
BanII G_rGCy'C
Cuts at: 2537 2953 3529 4559 2537
Size: 416 576 1030 4493
Fragments arranged by size:
4493 1030 576 416
BbeI G_GCGC'C
Cuts at: 1715 2760 3335 3949 4197 1715
Size: 1045 575 614 248 4033
Fragments arranged by size:
4033 1045 614 575 248
BbsI GAAGACnn'nnnn_
Cuts at: 1373 1463 1373
Size: 90 6425
BbvCI CC'TCA_GC
Cuts at: 1280 1892 2647 3499 1280
Size: 612 755 852 4296
Fragments arranged by size:
4296 852 755 612
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2217 2251 2217
Size: 34 6481
BciVI GTATCCnnnnn_n'
Cuts at: 4413 5174 6515 4413
Size: 761 1341 4413
Fragments arranged by size:
4413 1341 761
BclI T'GATC_A
Cuts at: 2960 4035 2960
Size: 1075 5440
BglI GCCn_nnn'nGGC
Cuts at: 2439 3307 2439
Size: 868 5647
BglII A'GATC_T
Cuts at: 2177 2721 2967 4030 6067 2177
Size: 544 246 1063 2037 2625
Fragments arranged by size:
2625 2037 1063 544 246
BlpI GC'TnA_GC
Cuts at: 301 3627 301
Size: 3326 3189
Bme1580I G_kGCm'C
Cuts at: 152 1785 2435 4140 4233 5289 152
Size: 1633 650 1705 93 1056 1378
Fragments arranged by size:
1705 1633 1378 1056 650 93
BmgBI CAC'GTC
Cuts at: 1125 1125
Size: 6515
BmrI ACTGGGnnnn_n'
Cuts at: 1772 2717 2872 3727 3808 4143 6287 1772
Size: 945 155 855 81 335 2144 2000
Fragments arranged by size:
2144 2000 945 855 335 155 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1620 4882 1620
Size: 3262 3253
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 276 712 1071 1319 1325 1759 1784 2218
Size: 125 436 359 248 6 434 25 434
Cuts at: 2218 2242 2350 2720 151
Size: 24 108 370 3946
Fragments arranged by size:
3946 436 434 434 370 359 248 125
108 25 24 6
Bpu10I CC'TnA_GC
Cuts at: 733 1280 1892 2647 3380 3499 733
Size: 547 612 755 733 119 3749
Fragments arranged by size:
3749 755 733 612 547 119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3691 4570 5062 5360 3691
Size: 879 492 298 4846
Fragments arranged by size:
4846 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 649 1416 1895 6219 649
Size: 767 479 4324 945
Fragments arranged by size:
4324 945 767 479
BsaAI yAC'GTr
Cuts at: 3868 3868
Size: 6515
BsaBI GATnn'nnATC
Cuts at: 6 4029 6066 6
Size: 4023 2037 455
Fragments arranged by size:
4023 2037 455
BsaHI Gr'CG_yC
Cuts at: 923 1712 2674 2757 2996 3332 3946 4194 4896
Size: 789 962 83 239 336 614 248 702
Cuts at: 4896 6392 923
Size: 1496 1046
Fragments arranged by size:
1496 1046 962 789 702 614 336 248
239 83
BsaWI w'CCGG_w
Cuts at: 1904 3125 3372 3704 4225 4903 5177 5324 5735
Size: 1221 247 332 521 678 274 147 411
Cuts at: 5735 1904
Size: 2684
Fragments arranged by size:
2684 1221 678 521 411 332 274 247
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 274 304 1246 1276 1609 1639 1857 1887 2025
Size: 30 942 30 333 30 218 30 138
Cuts at: 2025 2055 274
Size: 30 4734
Fragments arranged by size:
4734 942 333 218 138 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 183 631 799 1603 1614 1756 2626 2833 183
Size: 448 168 804 11 142 870 207 3865
Fragments arranged by size:
3865 870 804 448 207 168 142 11
BseYI C'CCAG_C
Cuts at: 1070 1703 1772 2642 3267 3940 4698 5275 5771
Size: 633 69 870 625 673 758 577 496
Cuts at: 5771 1070
Size: 1814
Fragments arranged by size:
1814 870 758 673 633 625 577 496
69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 593 748 1463 2688 2902 343
Size: 250 155 715 1225 214 3956
Fragments arranged by size:
3956 1225 715 250 214 155
BsiEI CG_ry'CG
Cuts at: 104 2716 4057 4103 5311 104
Size: 2612 1341 46 1208 1308
Fragments arranged by size:
2612 1341 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 611 2537 2825 3029 3119 3529 4307 4497 5289
Size: 1926 288 204 90 410 778 190 792
Cuts at: 5289 611
Size: 1837
Fragments arranged by size:
1926 1837 792 778 410 288 204 190
90
BsiWI C'GTAC_G
Cuts at: 2606 2606
Size: 6515
BsmBI CGTCTCn'nnnn_
Cuts at: 1738 1738
Size: 6515
Bsp1286I G_dGCh'C
Cuts at: 152 611 1785 2435 2537 2825 2953 3029 3119
Size: 459 1174 650 102 288 128 76 90
Cuts at: 3119 3529 4140 4233 4307 4497 4559 5289 152
Size: 410 611 93 74 190 62 730 1378
Fragments arranged by size:
1378 1174 730 650 611 459 410 288
190 128 102 93 90 76 74 62
BspEI T'CCGG_A
Cuts at: 3125 5735 3125
Size: 2610 3905
BspHI T'CATG_A
Cuts at: 2129 5691 2129
Size: 3562 2953
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1308 1578 2011 2327 3200 3565 3779 4081 4462
Size: 270 433 316 873 365 214 302 381
Cuts at: 4462 1308
Size: 3361
Fragments arranged by size:
3361 873 433 381 365 316 302 270
214
BsrBI CCG'CTC
Cuts at: 50 1841 2797 4807 4861 6345 6510 50
Size: 1791 956 2010 54 1484 165 55
Fragments arranged by size:
2010 1791 1484 956 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3107 4427 3107
Size: 1320 5195
BsrFI r'CCGG_y
Cuts at: 2351 3372 4513 4694 4903 2351
Size: 1021 1141 181 209 3963
Fragments arranged by size:
3963 1141 1021 209 181
BssSI C'ACGA_G
Cuts at: 3118 4786 5144 3118
Size: 1668 358 4489
Fragments arranged by size:
4489 1668 358
BstAPI GCAn_nnn'nTGC
Cuts at: 126 903 2319 3892 126
Size: 777 1416 1573 2749
Fragments arranged by size:
2749 1573 1416 777
BstBI TT'CG_AA
Cuts at: 3281 4876 3281
Size: 1595 4920
BstXI CCAn_nnnn'nTGG
Cuts at: 1785 2946 1785
Size: 1161 5354
BstYI r'GATC_y
Cuts at: 1 2177 2721 2810 2967 3038 3122 3540 4007
Size: 2176 544 89 157 71 84 418 467
Cuts at: 4007 4030 4365 4611 5623 5709 5721 6067 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
2176 1012 544 467 449 418 346 335
246 157 89 86 84 71 23 12
Bsu36I CC'TnA_GG
Cuts at: 195 195
Size: 6515
BtgI C'CryG_G
Cuts at: 107 794 942 1617 2027 3230 107
Size: 687 148 675 410 1203 3392
Fragments arranged by size:
3392 1203 687 675 410 148
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2554 2568 4465 4646 4809 2554
Size: 14 1897 181 163 4260
Fragments arranged by size:
4260 1897 181 163 14
BtsI GCAGTG_nn'
Cuts at: 858 1142 3758 858
Size: 284 2616 3615
Fragments arranged by size:
3615 2616 284
ClaI AT'CG_AT
Cuts at: 3086 3086
Size: 6515
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1484 1519 3193 3228 1484
Size: 35 1674 35 4771
Fragments arranged by size:
4771 1674 35 35
DraI TTT'AAA
Cuts at: 3514 3514
Size: 6515
DrdI GACnn_nn'nnGTC
Cuts at: 1588 4221 5079 1588
Size: 2633 858 3024
Fragments arranged by size:
3024 2633 858
EaeI y'GGCC_r
Cuts at: 378 585 783 1235 1677 2565 4100 4274 4665
Size: 207 198 452 442 888 1535 174 391
Cuts at: 4665 4692 6037 378
Size: 27 1345 856
Fragments arranged by size:
1535 1345 888 856 452 442 391 207
198 174 27
EagI C'GGCC_G
Cuts at: 4100 4100
Size: 6515
EarI CTCTTCn'nnn_
Cuts at: 279 1172 1352 2303 4538 4748 279
Size: 893 180 951 2235 210 2046
Fragments arranged by size:
2235 2046 951 893 210 180
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1554 2370 4723 5033 5179 6325 1554
Size: 816 2353 310 146 1146 1744
Fragments arranged by size:
2353 1744 1146 816 310 146
EcoICRI GAG'CTC
Cuts at: 2535 3527 2535
Size: 992 5523
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 276 712 1071 1193 1319 1325 1759 1784
Size: 125 436 359 122 126 6 434 25
Cuts at: 1784 2218 2242 2350 2569 2720 2790 4340 4772
Size: 434 24 108 219 151 70 1550 432
Cuts at: 4772 5519 5787 151
Size: 747 268 879
Fragments arranged by size:
1550 879 747 436 434 434 432 359
268 219 151 126 125 122 108 70
25 24 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2386 2386
Size: 6515
EcoO109I rG'GnC_Cy
Cuts at: 514 804 1183 1590 1650 1697 2316 3150 3159
Size: 290 379 407 60 47 619 834 9
Cuts at: 3159 3189 3654 514
Size: 30 465 3375
Fragments arranged by size:
3375 834 619 465 407 379 290 60
47 30 9
EcoRI G'AATT_C
Cuts at: 3519 3519
Size: 6515
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 627 4534 627
Size: 3907 2608
FspI TGC'GCA
Cuts at: 640 4296 640
Size: 3656 2859
HaeII r_GCGC'y
Cuts at: 1715 1930 2760 2805 3335 3949 4197 5219 6198
Size: 215 830 45 530 614 248 1022 979
Cuts at: 6198 1715
Size: 2032
Fragments arranged by size:
2032 1022 979 830 614 530 248 215
45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1087 1119 1246 1278 1399 1431 2035 2067 2650
Size: 32 127 32 121 32 604 32 583
Cuts at: 2650 2682 2952 2984 3427 3459 4357 4387 4389
Size: 32 270 32 443 32 898 30 2
Cuts at: 4389 4419 1087
Size: 30 3183
Fragments arranged by size:
3183 898 604 583 443 270 127 121
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 336 927 1484 3554 336
Size: 591 557 2070 3297
Fragments arranged by size:
3297 2070 591 557
HpaI GTT'AAC
Cuts at: 1484 1484
Size: 6515
KasI G'GCGC_C
Cuts at: 1711 2756 3331 3945 4193 1711
Size: 1045 575 614 248 4033
Fragments arranged by size:
4033 1045 614 575 248
KpnI G_GTAC'C
Cuts at: 3535 3535
Size: 6515
MluI A'CGCG_T
Cuts at: 5948 5948
Size: 6515
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1205 2017 2078 3119 5186 5370 1205
Size: 812 61 1041 2067 184 2350
Fragments arranged by size:
2350 2067 1041 812 184 61
MscI TGG'CCA
Cuts at: 380 587 785 1237 1679 4276 380
Size: 207 198 452 442 2597 2619
Fragments arranged by size:
2619 2597 452 442 207 198
MslI CAynn'nnrTG
Cuts at: 386 595 1439 1469 1883 3452 4504 4631 386
Size: 209 844 30 414 1569 1052 127 2270
Fragments arranged by size:
2270 1569 1052 844 414 209 127 30
MspA1I CmG'CkG
Cuts at: 248 1241 1662 1675 1737 2559 2646 3343 3626
Size: 993 421 13 62 822 87 697 283
Cuts at: 3626 3940 4300 5313 5558 248
Size: 314 360 1013 245 1205
Fragments arranged by size:
1205 1013 993 822 697 421 360 314
283 245 87 62 13
NaeI GCC'GGC
Cuts at: 4696 4696
Size: 6515
NarI GG'CG_CC
Cuts at: 1712 2757 3332 3946 4194 1712
Size: 1045 575 614 248 4033
Fragments arranged by size:
4033 1045 614 575 248
NcoI C'CATG_G
Cuts at: 107 942 2027 107
Size: 835 1085 4595
Fragments arranged by size:
4595 1085 835
NgoMIV G'CCGG_C
Cuts at: 4694 4694
Size: 6515
NspI r_CATG'y
Cuts at: 309 1734 1759 3568 4068 4975 309
Size: 1425 25 1809 500 907 1849
Fragments arranged by size:
1849 1809 1425 907 500 25
PasI CC'CwG_GG
Cuts at: 2451 2451
Size: 6515
PciI A'CATG_T
Cuts at: 4971 4971
Size: 6515
PflMI CCAn_nnn'nTGG
Cuts at: 1224 2289 1224
Size: 1065 5450
PfoI T'CCnGG_A
Cuts at: 178 1761 2813 178
Size: 1583 1052 3880
Fragments arranged by size:
3880 1583 1052
PmeI GTTT'AAAC
Cuts at: 3514 3514
Size: 6515
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1946 1978 5654 5686 1946
Size: 32 3676 32 2775
Fragments arranged by size:
3676 2775 32 32
PpuMI rG'GwC_Cy
Cuts at: 804 1183 1590 1650 1697 2316 3150 3159 3189
Size: 379 407 60 47 619 834 9 30
Cuts at: 3189 804
Size: 4130
Fragments arranged by size:
4130 834 619 407 379 60 47 30
9
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3232 3264 3232
Size: 32 6483
PstI C_TGCA'G
Cuts at: 915 2062 2240 2462 3197 3308 3342 3562 4247
Size: 1147 178 222 735 111 34 220 685
Cuts at: 4247 915
Size: 3183
Fragments arranged by size:
3183 1147 735 685 222 220 178 111
34
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6515
PvuII CAG'CTG
Cuts at: 1662 1737 2559 2646 3343 3940 4300 1662
Size: 75 822 87 697 597 360 3877
Fragments arranged by size:
3877 822 697 597 360 87 75
RsrII CG'GwC_CG
Cuts at: 4710 4710
Size: 6515
SacI G_AGCT'C
Cuts at: 2537 3529 2537
Size: 992 5523
SalI G'TCGA_C
Cuts at: 3552 3552
Size: 6515
SanDI GG'GwC_CC
Cuts at: 1183 1697 1183
Size: 514 6001
SapI GCTCTTCn'nnn_
Cuts at: 279 2303 4538 4748 279
Size: 2024 2235 210 2046
Fragments arranged by size:
2235 2046 2024 210
SbfI CC_TGCA'GG
Cuts at: 3308 3562 3308
Size: 254 6261
ScaI AGT'ACT
Cuts at: 6032 6032
Size: 6515
SfcI C'TryA_G
Cuts at: 33 911 2058 2236 2458 3193 3304 3338 3558
Size: 878 1147 178 222 735 111 34 220
Cuts at: 3558 4243 5236 5427 33
Size: 685 993 191 1121
Fragments arranged by size:
1147 1121 993 878 735 685 222 220
191 178 111 34
SfiI GGCCn_nnn'nGGCC
Cuts at: 3307 3307
Size: 6515
SfoI GGC'GCC
Cuts at: 1713 2758 3333 3947 4195 1713
Size: 1045 575 614 248 4033
Fragments arranged by size:
4033 1045 614 575 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6515
SgrAI Cr'CCGG_yG
Cuts at: 2351 2351
Size: 6515
SmaI CCC'GGG
Cuts at: 1450 3537 1450
Size: 2087 4428
SmlI C'TyrA_G
Cuts at: 3670 4585 5077 5339 3670
Size: 915 492 262 4846
Fragments arranged by size:
4846 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6515
SphI G_CATG'C
Cuts at: 1734 1759 3568 4068 1734
Size: 25 1809 500 4181
Fragments arranged by size:
4181 1809 500 25
StuI AGG'CCT
Cuts at: 1314 1610 1820 3277 1314
Size: 296 210 1457 4552
Fragments arranged by size:
4552 1457 296 210
StyI C'CwwG_G
Cuts at: 107 942 1315 1426 2027 2387 3185 3649 4003
Size: 835 373 111 601 360 798 464 354
Cuts at: 4003 107
Size: 2619
Fragments arranged by size:
2619 835 798 601 464 373 360 354
111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 901 2025 4098 4231 4690 4899 901
Size: 1124 2073 133 459 209 2517
Fragments arranged by size:
2517 2073 1124 459 209 133
TatI w'GTAC_w
Cuts at: 1039 6030 1039
Size: 4991 1524
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 274 306 1609 1641 1857 1889 2025 2057 6032
Size: 32 1303 32 216 32 136 32 3975
Cuts at: 6032 6064 274
Size: 32 725
Fragments arranged by size:
3975 1303 725 216 136 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2278 2467 4312 2278
Size: 189 1845 4481
Fragments arranged by size:
4481 1845 189
XbaI T'CTAG_A
Cuts at: 3546 3546
Size: 6515
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1734 2885 4010 1734
Size: 1151 1125 4239
Fragments arranged by size:
4239 1151 1125
XmaI C'CCGG_G
Cuts at: 1448 3535 1448
Size: 2087 4428
ZraI GAC'GTC
Cuts at: 924 2675 924
Size: 1751 4764
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PsrI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AflII AhdI AloI ApaI AscI BfrBI BmtI
BsmI BsrGI BssHII BstEII BstZ17I DraIII EcoRV FseI
FspAI HindIII MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PshAI PsiI PspOMI PspXI SacII SexAI
SnaBI SrfI SspI SwaI XhoI XmnI