Restriction map for
pF1KA0232
(Circular) (Six-base) MAPSORT of: pF1KA0232.seq Check: 4599 from: 1 to: 7298
pF1KA0232 7298 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1994 1994
Size: 7298
AccI GT'mk_AC
Cuts at: 4336 4336
Size: 7298
Acc65I G'GTAC_C
Cuts at: 4314 4314
Size: 7298
AclI AA'CG_TT
Cuts at: 7138 7138
Size: 7298
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 965 1271 2704 2720 3308 5123 5555 6302
Size: 451 306 1433 16 588 1815 432 747
Cuts at: 6302 6570 514
Size: 268 1242
Fragments arranged by size:
1815 1433 1242 747 588 451 432 306
268 16
AflII C'TTAA_G
Cuts at: 3237 3237
Size: 7298
AflIII A'CryG_T
Cuts at: 2266 4650 5754 6731 2266
Size: 2384 1104 977 2833
Fragments arranged by size:
2833 2384 1104 977
AgeI A'CCGG_T
Cuts at: 5686 5686
Size: 7298
AleI CACnn'nnGTG
Cuts at: 832 832
Size: 7298
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1776 1776
Size: 7298
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2226 2258 2226
Size: 32 7266
AlwNI CAG_nnn'CTG
Cuts at: 178 2998 4603 6170 178
Size: 2820 1605 1567 1306
Fragments arranged by size:
2820 1605 1567 1306
ApaI G_GGCC'C
Cuts at: 4258 4258
Size: 7298
ApaLI G'TGCA_C
Cuts at: 6068 6068
Size: 7298
ApoI r'AATT_y
Cuts at: 880 2517 2613 3481 3628 4302 6651 6907 880
Size: 1637 96 868 147 674 2349 256 1271
Fragments arranged by size:
2349 1637 1271 868 674 256 147 96
AseI AT'TA_AT
Cuts at: 20 2021 2333 3827 20
Size: 2001 312 1494 3491
Fragments arranged by size:
3491 2001 1494 312
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7298
AvaI C'yCGr_G
Cuts at: 247 4318 247
Size: 4071 3227
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3011 3044 3011
Size: 33 7265
BamHI G'GATC_C
Cuts at: 1665 4323 1665
Size: 2658 4640
BanI G'GyrC_C
Cuts at: 1672 4314 4728 4976 5011 1672
Size: 2642 414 248 35 3959
Fragments arranged by size:
3959 2642 414 248 35
BanII G_rGCy'C
Cuts at: 248 509 1307 2009 4258 4274 4312 5342 248
Size: 261 798 702 2249 16 38 1030 2204
Fragments arranged by size:
2249 2204 1030 798 702 261 38 16
BbeI G_GCGC'C
Cuts at: 4732 4980 4732
Size: 248 7050
BbsI GAAGACnn'nnnn_
Cuts at: 289 448 965 3170 4112 289
Size: 159 517 2205 942 3475
Fragments arranged by size:
3475 2205 942 517 159
BbvCI CC'TCA_GC
Cuts at: 1835 1835
Size: 7298
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1309 1343 1309
Size: 34 7264
BciVI GTATCCnnnnn_n'
Cuts at: 1386 5196 5957 7298 1386
Size: 3810 761 1341 1386
Fragments arranged by size:
3810 1386 1341 761
BclI T'GATC_A
Cuts at: 1650 3105 4818 1650
Size: 1455 1713 4130
Fragments arranged by size:
4130 1713 1455
BglII A'GATC_T
Cuts at: 411 3555 4813 6850 411
Size: 3144 1258 2037 859
Fragments arranged by size:
3144 2037 1258 859
BlpI GC'TnA_GC
Cuts at: 4410 4410
Size: 7298
Bme1580I G_kGCm'C
Cuts at: 3065 4258 4923 5016 6072 3065
Size: 1193 665 93 1056 4291
Fragments arranged by size:
4291 1193 1056 665 93
BmrI ACTGGGnnnn_n'
Cuts at: 1568 3305 3713 4076 4510 4591 4926 7070 1568
Size: 1737 408 363 434 81 335 2144 1796
Fragments arranged by size:
2144 1796 1737 434 408 363 335 81
BmtI G_CTAG'C
Cuts at: 755 755
Size: 7298
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 528 4245 5665 528
Size: 3717 1420 2161
Fragments arranged by size:
3717 2161 1420
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 498 566 587 966 1922 2731 2806 4181 4182
Size: 68 21 379 956 809 75 1375 1
Cuts at: 4182 498
Size: 3614
Fragments arranged by size:
3614 1375 956 809 379 75 68 21
1
Bpu10I CC'TnA_GC
Cuts at: 1835 2571 1835
Size: 736 6562
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 144 981 3509 3637 4474 5353 5845 6143 144
Size: 837 2528 128 837 879 492 298 1299
Fragments arranged by size:
2528 1299 879 837 837 492 298 128
BsaI GGTCTCn'nnnn_
Cuts at: 578 1203 1363 2707 7002 578
Size: 625 160 1344 4295 874
Fragments arranged by size:
4295 1344 874 625 160
BsaAI yAC'GTr
Cuts at: 4651 4651
Size: 7298
BsaBI GATnn'nnATC
Cuts at: 6 1610 1649 4812 6849 6
Size: 1604 39 3163 2037 455
Fragments arranged by size:
3163 2037 1604 455 39
BsaHI Gr'CG_yC
Cuts at: 4729 4977 5679 7175 4729
Size: 248 702 1496 4852
Fragments arranged by size:
4852 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1900 4487 5008 5686 5960 6107 6518 1900
Size: 2587 521 678 274 147 411 2680
Fragments arranged by size:
2680 2587 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 753 783 1294 1324 753
Size: 30 511 30 6727
Fragments arranged by size:
6727 511 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 147 517 1315 1943 2915 3407 3479 3980 4282
Size: 370 798 628 972 492 72 501 302
Cuts at: 4282 147
Size: 3163
Fragments arranged by size:
3163 972 798 628 501 492 370 302
72
BseYI C'CCAG_C
Cuts at: 234 359 4723 5481 6058 6554 234
Size: 125 4364 758 577 496 978
Fragments arranged by size:
4364 978 758 577 496 125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 3011 244
Size: 2767 4531
BsiEI CG_ry'CG
Cuts at: 104 4840 4886 6094 104
Size: 4736 46 1208 1308
Fragments arranged by size:
4736 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 248 509 1249 1976 2009 3672 4198 4274 4312
Size: 261 740 727 33 1663 526 76 38
Cuts at: 4312 5090 5280 6072 248
Size: 778 190 792 1474
Fragments arranged by size:
1663 1474 792 778 740 727 526 261
190 76 38 33
BsmI GAATG_Cn'
Cuts at: 3277 3277
Size: 7298
Bsp1286I G_dGCh'C
Cuts at: 248 509 1249 1307 1976 2009 3065 3672 4198
Size: 261 740 58 669 33 1056 607 526
Cuts at: 4198 4258 4274 4312 4923 5016 5090 5280 5342
Size: 60 16 38 611 93 74 190 62
Cuts at: 5342 6072 248
Size: 730 1474
Fragments arranged by size:
1474 1056 740 730 669 611 607 526
261 190 93 74 62 60 58 38
33 16
BspEI T'CCGG_A
Cuts at: 6518 6518
Size: 7298
BspHI T'CATG_A
Cuts at: 680 6474 680
Size: 5794 1504
BspMI ACCTGCnnnn'nnnn_
Cuts at: 335 1483 1994 3841 4348 4562 4864 5245 335
Size: 1148 511 1847 507 214 302 381 2388
Fragments arranged by size:
2388 1847 1148 511 507 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5590 5644 7128 7293 50
Size: 5540 54 1484 165 55
Fragments arranged by size:
5540 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2074 5210 2074
Size: 3136 4162
BsrFI r'CCGG_y
Cuts at: 5296 5477 5686 5296
Size: 181 209 6908
Fragments arranged by size:
6908 209 181
BsrGI T'GTAC_A
Cuts at: 121 917 1265 4141 121
Size: 796 348 2876 3278
Fragments arranged by size:
3278 2876 796 348
BssSI C'ACGA_G
Cuts at: 966 1064 5569 5927 966
Size: 98 4505 358 2337
Fragments arranged by size:
4505 2337 358 98
BstAPI GCAn_nnn'nTGC
Cuts at: 4675 4675
Size: 7298
BstBI TT'CG_AA
Cuts at: 1074 5659 1074
Size: 4585 2713
BstXI CCAn_nnnn'nTGG
Cuts at: 3601 3601
Size: 7298
BstYI r'GATC_y
Cuts at: 1 411 1236 1665 2029 3201 3410 3543 3555
Size: 410 825 429 364 1172 209 133 12
Cuts at: 3555 4323 4790 4813 5148 5394 6406 6492 6504
Size: 768 467 23 335 246 1012 86 12
Cuts at: 6504 6850 1
Size: 346 449
Fragments arranged by size:
1172 1012 825 768 467 449 429 410
364 346 335 246 209 133 86 23
12 12
Bsu36I CC'TnA_GG
Cuts at: 773 773
Size: 7298
BtgI C'CryG_G
Cuts at: 2245 4182 2245
Size: 1937 5361
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5248 5429 5592 5248
Size: 181 163 6954
Fragments arranged by size:
6954 181 163
BtsI GCAGTG_nn'
Cuts at: 822 1179 1424 4541 822
Size: 357 245 3117 3579
Fragments arranged by size:
3579 3117 357 245
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2600 2635 2600
Size: 35 7263
DraI TTT'AAA
Cuts at: 1217 3318 4297 1217
Size: 2101 979 4218
Fragments arranged by size:
4218 2101 979
DrdI GACnn_nn'nnGTC
Cuts at: 5004 5862 5004
Size: 858 6440
EaeI y'GGCC_r
Cuts at: 4883 5057 5448 5475 6820 4883
Size: 174 391 27 1345 5361
Fragments arranged by size:
5361 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4883 4883
Size: 7298
EarI CTCTTCn'nnn_
Cuts at: 539 729 3109 3899 4085 4214 4259 5321 5531
Size: 190 2380 790 186 129 45 1062 210
Cuts at: 5531 539
Size: 2306
Fragments arranged by size:
2380 2306 1062 790 210 190 186 129
45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1104 4253 5506 5816 5962 7108 1104
Size: 3149 1253 310 146 1146 1294
Fragments arranged by size:
3149 1294 1253 1146 310 146
EcoICRI GAG'CTC
Cuts at: 246 507 2007 4272 4310 246
Size: 261 1500 2265 38 3234
Fragments arranged by size:
3234 2265 1500 261 38
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 498 514 566 587 965 966 1271 1922 2704
Size: 16 52 21 378 1 305 651 782
Cuts at: 2704 2720 2731 2806 3308 4181 4182 5123 5555
Size: 16 11 75 502 873 1 941 432
Cuts at: 5555 6302 6570 498
Size: 747 268 1226
Fragments arranged by size:
1226 941 873 782 747 651 502 432
378 305 268 75 52 21 16 16
11 1 1
EcoO109I rG'GnC_Cy
Cuts at: 175 1562 1854 4255 4437 175
Size: 1387 292 2401 182 3036
Fragments arranged by size:
3036 2401 1387 292 182
EcoRI G'AATT_C
Cuts at: 4302 4302
Size: 7298
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5317 5317
Size: 7298
FspI TGC'GCA
Cuts at: 5079 5079
Size: 7298
HaeII r_GCGC'y
Cuts at: 4098 4205 4732 4980 6002 6981 4098
Size: 107 527 248 1022 979 4415
Fragments arranged by size:
4415 1022 979 527 248 107
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 230 262 264 296 463 495 751 783 945
Size: 32 2 32 167 32 256 32 162
Cuts at: 945 977 1221 1253 1393 1425 3402 3434 3604
Size: 32 244 32 140 32 1977 32 170
Cuts at: 3604 3636 5140 5170 5172 5202 230
Size: 32 1504 30 2 30 2326
Fragments arranged by size:
2326 1977 1504 256 244 170 167 162
140 32 32 32 32 32 32 32
32 32 30 30 2 2
HincII GTy'rAC
Cuts at: 3384 4337 3384
Size: 953 6345
KasI G'GCGC_C
Cuts at: 4728 4976 4728
Size: 248 7050
KpnI G_GTAC'C
Cuts at: 4318 4318
Size: 7298
MfeI C'AATT_G
Cuts at: 896 1325 896
Size: 429 6869
MluI A'CGCG_T
Cuts at: 6731 6731
Size: 7298
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 957 1643 1681 5969 6153 957
Size: 686 38 4288 184 2102
Fragments arranged by size:
4288 2102 686 184 38
MscI TGG'CCA
Cuts at: 5059 5059
Size: 7298
MslI CAynn'nnrTG
Cuts at: 685 832 1634 2535 2558 3599 4008 5287 5414
Size: 147 802 901 23 1041 409 1279 127
Cuts at: 5414 685
Size: 2569
Fragments arranged by size:
2569 1279 1041 901 802 409 147 127
23
MspA1I CmG'CkG
Cuts at: 1191 3900 4184 4409 4723 5083 6096 6341 1191
Size: 2709 284 225 314 360 1013 245 2148
Fragments arranged by size:
2709 2148 1013 360 314 284 245 225
NaeI GCC'GGC
Cuts at: 5479 5479
Size: 7298
NarI GG'CG_CC
Cuts at: 4729 4977 4729
Size: 248 7050
NcoI C'CATG_G
Cuts at: 2245 2245
Size: 7298
NgoMIV G'CCGG_C
Cuts at: 5477 5477
Size: 7298
NheI G'CTAG_C
Cuts at: 751 751
Size: 7298
NspI r_CATG'y
Cuts at: 4351 4851 5758 4351
Size: 500 907 5891
Fragments arranged by size:
5891 907 500
PasI CC'CwG_GG
Cuts at: 2858 2858
Size: 7298
PciI A'CATG_T
Cuts at: 5754 5754
Size: 7298
PfoI T'CCnGG_A
Cuts at: 3204 3204
Size: 7298
PmeI GTTT'AAAC
Cuts at: 4297 4297
Size: 7298
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6437 6469 6437
Size: 32 7266
PpuMI rG'GwC_Cy
Cuts at: 1562 1854 1562
Size: 292 7006
PsiI TTA'TAA
Cuts at: 377 1537 377
Size: 1160 6138
PspOMI G'GGCC_C
Cuts at: 4254 4254
Size: 7298
PstI C_TGCA'G
Cuts at: 332 1533 1853 4345 5030 332
Size: 1201 320 2492 685 2600
Fragments arranged by size:
2600 2492 1201 685 320
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7298
PvuII CAG'CTG
Cuts at: 1191 3900 4723 5083 1191
Size: 2709 823 360 3406
Fragments arranged by size:
3406 2709 823 360
RsrII CG'GwC_CG
Cuts at: 5493 5493
Size: 7298
SacI G_AGCT'C
Cuts at: 248 509 2009 4274 4312 248
Size: 261 1500 2265 38 3234
Fragments arranged by size:
3234 2265 1500 261 38
SacII CC_GC'GG
Cuts at: 4185 4185
Size: 7298
SalI G'TCGA_C
Cuts at: 4335 4335
Size: 7298
SapI GCTCTTCn'nnn_
Cuts at: 729 4085 5321 5531 729
Size: 3356 1236 210 2496
Fragments arranged by size:
3356 2496 1236 210
SbfI CC_TGCA'GG
Cuts at: 332 1853 4345 332
Size: 1521 2492 3285
Fragments arranged by size:
3285 2492 1521
ScaI AGT'ACT
Cuts at: 6815 6815
Size: 7298
SexAI A'CCwGG_T
Cuts at: 3964 3964
Size: 7298
SfcI C'TryA_G
Cuts at: 33 328 1529 1849 2565 2624 4341 5026 6019
Size: 295 1201 320 716 59 1717 685 993
Cuts at: 6019 6210 33
Size: 191 1121
Fragments arranged by size:
1717 1201 1121 993 716 685 320 295
191 59
SfoI GGC'GCC
Cuts at: 4730 4978 4730
Size: 248 7050
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7298
SmaI CCC'GGG
Cuts at: 4320 4320
Size: 7298
SmlI C'TyrA_G
Cuts at: 159 247 996 3237 3524 3616 4453 5368 5860
Size: 88 749 2241 287 92 837 915 492
Cuts at: 5860 6122 159
Size: 262 1335
Fragments arranged by size:
2241 1335 915 837 749 492 287 262
92 88
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7298
SphI G_CATG'C
Cuts at: 4351 4851 4351
Size: 500 6798
SspI AAT'ATT
Cuts at: 1336 2292 3510 1336
Size: 956 1218 5124
Fragments arranged by size:
5124 1218 956
StyI C'CwwG_G
Cuts at: 803 1857 2245 4034 4432 4786 803
Size: 1054 388 1789 398 354 3315
Fragments arranged by size:
3315 1789 1054 398 388 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 4095 4239 4881 5014 5473 5682 4095
Size: 144 642 133 459 209 5711
Fragments arranged by size:
5711 642 459 209 144 133
TatI w'GTAC_w
Cuts at: 121 626 917 1265 3983 4141 6813 121
Size: 505 291 348 2718 158 2672 606
Fragments arranged by size:
2718 2672 606 505 348 291 158
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1294 1326 3363 3395 6815 6847 1294
Size: 32 2037 32 3420 32 1745
Fragments arranged by size:
3420 2037 1745 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5095 5095
Size: 7298
XbaI T'CTAG_A
Cuts at: 420 2164 2382 2691 4329 420
Size: 1744 218 309 1638 3389
Fragments arranged by size:
3389 1744 1638 309 218
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 227 4793 227
Size: 4566 2732
XhoI C'TCGA_G
Cuts at: 247 247
Size: 7298
XmaI C'CCGG_G
Cuts at: 4318 4318
Size: 7298
XmnI GAAnn'nnTTC
Cuts at: 638 2378 3082 3359 638
Size: 1740 704 277 4577
Fragments arranged by size:
4577 1740 704 277
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PasI PciI PfoI PmeI PpiI PpuMI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AscI AvrII BfrBI BglI BmgBI
BsiWI BsmBI BssHII BstEII BstZ17I ClaI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI NdeI NotI NruI
NsiI PacI PflMI PmlI PshAI PspXI PsrI SanDI
SfiI SgrAI SnaBI SrfI StuI SwaI ZraI