Restriction map for
pF1KA0233
(Circular) (Six-base) MAPSORT of: pF1KA0233.seq Check: 3926 from: 1 to: 10676
pF1KA0233 10676 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2415 2505 2912 3438 2415
Size: 90 407 526 9653
Fragments arranged by size:
9653 526 407 90
AatII G_ACGT'C
Cuts at: 2501 5987 2501
Size: 3486 7190
AccI GT'mk_AC
Cuts at: 206 773 1904 1916 1958 2039 5718 6017 7714
Size: 567 1131 12 42 81 3679 299 1697
Cuts at: 7714 206
Size: 3168
Fragments arranged by size:
3679 3168 1697 1131 567 299 81 42
12
Acc65I G'GTAC_C
Cuts at: 6105 7692 6105
Size: 1587 9089
AclI AA'CG_TT
Cuts at: 10516 10516
Size: 10676
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 463 825 1744 2453 2608 3601 3852 4427
Size: 292 362 919 709 155 993 251 575
Cuts at: 4427 7467 8501 8933 9680 9948 171
Size: 3040 1034 432 747 268 899
Fragments arranged by size:
3040 1034 993 919 899 747 709 575
432 362 292 268 251 155
AfeI AGC'GCT
Cuts at: 4747 5290 4747
Size: 543 10133
AflIII A'CryG_T
Cuts at: 1553 1919 2246 2508 7439 8028 9132 10109 1553
Size: 366 327 262 4931 589 1104 977 2120
Fragments arranged by size:
4931 2120 1104 977 589 366 327 262
AgeI A'CCGG_T
Cuts at: 9064 9064
Size: 10676
AhdI GACnn_n'nnGTC
Cuts at: 6081 6081
Size: 10676
AleI CACnn'nnGTG
Cuts at: 2425 2425
Size: 10676
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2179 2809 3730 6202 7375 7486 2179
Size: 630 921 2472 1173 111 5369
Fragments arranged by size:
5369 2472 1173 921 630 111
AlwNI CAG_nnn'CTG
Cuts at: 378 398 851 927 1797 1809 2762 2820 3030
Size: 20 453 76 870 12 953 58 210
Cuts at: 3030 3042 3267 3977 4647 5028 5508 6861 7981
Size: 12 225 710 670 381 480 1353 1120
Cuts at: 7981 9548 378
Size: 1567 1506
Fragments arranged by size:
1567 1506 1353 1120 953 870 710 670
480 453 381 225 210 76 58 20
12 12
ApaI G_GGCC'C
Cuts at: 1600 2837 3387 3738 4246 4274 4585 5749 7205
Size: 1237 550 351 508 28 311 1164 1456
Cuts at: 7205 1600
Size: 5071
Fragments arranged by size:
5071 1456 1237 1164 550 508 351 311
28
ApaLI G'TGCA_C
Cuts at: 1261 4786 9446 1261
Size: 3525 4660 2491
Fragments arranged by size:
4660 3525 2491
ApoI r'AATT_y
Cuts at: 3085 7680 10029 10285 3085
Size: 4595 2349 256 3476
Fragments arranged by size:
4595 3476 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 10676
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10676
AvaI C'yCGr_G
Cuts at: 833 1157 1324 4413 4585 4990 5274 6109 7132
Size: 324 167 3089 172 405 284 835 1023
Cuts at: 7132 7577 7696 833
Size: 445 119 3813
Fragments arranged by size:
3813 3089 1023 835 445 405 324 284
172 167 119
BamHI G'GATC_C
Cuts at: 7701 7701
Size: 10676
BanI G'GyrC_C
Cuts at: 527 874 1221 1657 2163 2427 2948 3016 3194
Size: 347 347 436 506 264 521 68 178
Cuts at: 3194 4709 4717 5008 5063 5604 6105 6441 7138
Size: 1515 8 291 55 541 501 336 697
Cuts at: 7138 7692 8106 8354 8389 527
Size: 554 414 248 35 2814
Fragments arranged by size:
2814 1515 697 554 541 521 506 501
436 414 347 347 336 291 264 248
178 68 55 35 8
BanII G_rGCy'C
Cuts at: 556 1285 1325 1600 1781 2147 2283 2774 2837
Size: 729 40 275 181 366 136 491 63
Cuts at: 2837 3387 3533 3738 4246 4274 4291 4585 4769
Size: 550 146 205 508 28 17 294 184
Cuts at: 4769 5690 5749 6983 7205 7690 8720 556
Size: 921 59 1234 222 485 1030 2512
Fragments arranged by size:
2512 1234 1030 921 729 550 508 491
485 366 294 275 222 205 184 181
146 136 63 59 40 28 17
BbeI G_GCGC'C
Cuts at: 531 878 2952 5067 8110 8358 531
Size: 347 2074 2115 3043 248 2849
Fragments arranged by size:
3043 2849 2115 2074 347 248
BbsI GAAGACnn'nnnn_
Cuts at: 3585 3813 4002 4415 5241 5304 5446 5630 3585
Size: 228 189 413 826 63 142 184 8631
Fragments arranged by size:
8631 826 413 228 189 184 142 63
BbvCI CC'TCA_GC
Cuts at: 304 4483 5780 304
Size: 4179 1297 5200
Fragments arranged by size:
5200 4179 1297
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1854 1888 5971 6005 6309 6343 1854
Size: 34 4083 34 304 34 6187
Fragments arranged by size:
6187 4083 304 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4186 7276 8574 9335 10676 4186
Size: 3090 1298 761 1341 4186
Fragments arranged by size:
4186 3090 1341 1298 761
BclI T'GATC_A
Cuts at: 7646 8196 7646
Size: 550 10126
BglI GCCn_nnn'nGGC
Cuts at: 1121 1305 4305 5934 6597 1121
Size: 184 3000 1629 663 5200
Fragments arranged by size:
5200 3000 1629 663 184
BglII A'GATC_T
Cuts at: 341 3098 7490 8191 10228 341
Size: 2757 4392 701 2037 789
Fragments arranged by size:
4392 2757 2037 789 701
BlpI GC'TnA_GC
Cuts at: 1319 2462 3461 4630 7788 1319
Size: 1143 999 1169 3158 4207
Fragments arranged by size:
4207 3158 1169 1143 999
Bme1580I G_kGCm'C
Cuts at: 362 1226 1265 1600 2168 2837 3387 3545 3738
Size: 864 39 335 568 669 550 158 193
Cuts at: 3738 4246 4274 4585 4790 5749 5920 6966 7141
Size: 508 28 311 205 959 171 1046 175
Cuts at: 7141 7194 7205 8301 8394 9450 362
Size: 53 11 1096 93 1056 1588
Fragments arranged by size:
1588 1096 1056 1046 959 864 669 568
550 508 335 311 205 193 175 171
158 93 53 39 28 11
BmgBI CAC'GTC
Cuts at: 4035 4731 6083 4035
Size: 696 1352 8628
Fragments arranged by size:
8628 1352 696
BmrI ACTGGGnnnn_n'
Cuts at: 6486 7888 7969 8304 10448 6486
Size: 1402 81 335 2144 6714
Fragments arranged by size:
6714 2144 1402 335 81
BmtI G_CTAG'C
Cuts at: 1174 4069 1174
Size: 2895 7781
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 426 2166 2394 5556 6942 7158 9043 426
Size: 1740 228 3162 1386 216 1885 2059
Fragments arranged by size:
3162 2059 1885 1740 1386 228 216
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 750 981 1186 1645 1724 2136 2143 2364
Size: 428 231 205 459 79 412 7 221
Cuts at: 2364 2494 2557 3115 3339 3532 4033 4056 4282
Size: 130 63 558 224 193 501 23 226
Cuts at: 4282 5104 5455 5876 7606 7612 322
Size: 822 351 421 1730 6 3386
Fragments arranged by size:
3386 1730 822 558 501 459 428 421
412 351 231 226 224 221 205 193
130 79 63 23 7 6
Bpu10I CC'TnA_GC
Cuts at: 304 1577 4015 4483 4939 5689 5780 304
Size: 1273 2438 468 456 750 91 5200
Fragments arranged by size:
5200 2438 1273 750 468 456 91
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 952 1969 2713 3040 4090 6733 7525 7852 8731
Size: 1017 744 327 1050 2643 792 327 879
Cuts at: 8731 9223 9521 952
Size: 492 298 2107
Fragments arranged by size:
2643 2107 1050 1017 879 792 744 492
327 327 298
BsaI GGTCTCn'nnnn_
Cuts at: 397 504 970 3917 7633 10380 397
Size: 107 466 2947 3716 2747 693
Fragments arranged by size:
3716 2947 2747 693 466 107
BsaAI yAC'GTr
Cuts at: 120 1218 1554 2247 5379 7440 8029 120
Size: 1098 336 693 3132 2061 589 2767
Fragments arranged by size:
3132 2767 2061 1098 693 589 336
BsaBI GATnn'nnATC
Cuts at: 6 8190 10227 6
Size: 8184 2037 455
Fragments arranged by size:
8184 2037 455
BsaHI Gr'CG_yC
Cuts at: 528 875 1058 1333 2498 2949 3494 5064 5984
Size: 347 183 275 1165 451 545 1570 920
Cuts at: 5984 8107 8355 9057 10553 528
Size: 2123 248 702 1496 651
Fragments arranged by size:
2123 1570 1496 1165 920 702 651 545
451 347 275 248 183
BsaWI w'CCGG_w
Cuts at: 7635 7865 8386 9064 9338 9485 9896 7635
Size: 230 521 678 274 147 411 8415
Fragments arranged by size:
8415 678 521 411 274 230 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1102 1132 2233 2263 4751 4781 5849 5879 7040
Size: 30 1101 30 2488 30 1068 30 1161
Cuts at: 7040 7070 7507 7537 7585 7588 7615 7618 1102
Size: 30 437 30 48 3 27 3 4160
Fragments arranged by size:
4160 2488 1161 1101 1068 437 48 30
30 30 30 30 30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 492 2338 2371 2374 2377 2380 2383 2386 3487
Size: 1846 33 3 3 3 3 3 1101
Cuts at: 3487 3964 4150 4281 4405 4408 4603 4744 4912
Size: 477 186 131 124 3 195 141 168
Cuts at: 4912 4987 5101 5548 5587 5590 5605 6844 7255
Size: 75 114 447 39 3 15 1239 411
Cuts at: 7255 7528 7609 7612 492
Size: 273 81 3 3556
Fragments arranged by size:
3556 1846 1239 1101 477 447 411 273
195 186 168 141 131 124 114 81
75 39 33 15 3 3 3 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 297 385 398 703 960 1617 2106 2276 2394
Size: 88 13 305 257 657 489 170 118
Cuts at: 2394 3437 3666 3851 4151 4555 4656 4887 5281
Size: 1043 229 185 300 404 101 231 394
Cuts at: 5281 5501 5748 6189 6296 6753 6788 6854 6955
Size: 220 247 441 107 457 35 66 101
Cuts at: 6955 8101 8859 9436 9932 297
Size: 1146 758 577 496 1041
Fragments arranged by size:
1146 1043 1041 758 657 577 496 489
457 441 404 394 305 300 257 247
231 229 220 185 170 118 107 101
101 88 66 35 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 783 2185 2533 2748 3142 4015 4639 5491 5941
Size: 1402 348 215 394 873 624 852 450
Cuts at: 5941 7091 783
Size: 1150 4368
Fragments arranged by size:
4368 1402 1150 873 852 624 450 394
348 215
BsiEI CG_ry'CG
Cuts at: 104 1893 3507 5876 5929 6998 8218 8264 9472
Size: 1789 1614 2369 53 1069 1220 46 1208
Cuts at: 9472 104
Size: 1308
Fragments arranged by size:
2369 1789 1614 1308 1220 1208 1069 53
46
BsiHKAI G_wGCw'C
Cuts at: 125 1265 1285 1507 1631 2147 2246 2960 4636
Size: 1140 20 222 124 516 99 714 1676
Cuts at: 4636 4769 4790 4945 5246 5690 6983 7690 8468
Size: 133 21 155 301 444 1293 707 778
Cuts at: 8468 8658 9450 125
Size: 190 792 1351
Fragments arranged by size:
1676 1351 1293 1140 792 778 714 707
516 444 301 222 190 155 133 124
99 21 20
BsmBI CGTCTCn'nnnn_
Cuts at: 1340 1340
Size: 10676
Bsp1286I G_dGCh'C
Cuts at: 125 362 556 1226 1265 1285 1325 1507 1600
Size: 237 194 670 39 20 40 182 93
Cuts at: 1600 1631 1781 2147 2168 2246 2283 2774 2837
Size: 31 150 366 21 78 37 491 63
Cuts at: 2837 2960 3387 3533 3545 3738 4246 4274 4291
Size: 123 427 146 12 193 508 28 17
Cuts at: 4291 4585 4636 4769 4790 4945 5246 5690 5749
Size: 294 51 133 21 155 301 444 59
Cuts at: 5749 5920 6966 6983 7141 7194 7205 7690 8301
Size: 171 1046 17 158 53 11 485 611
Cuts at: 8301 8394 8468 8658 8720 9450 125
Size: 93 74 190 62 730 1351
Fragments arranged by size:
1351 1046 730 670 611 508 491 485
444 427 366 301 294 237 194 193
190 182 171 158 155 150 146 133
123 93 93 78 74 63 62 59
53 51 40 39 37 31 28 21
21 20 17 17 12 11
BspEI T'CCGG_A
Cuts at: 9896 9896
Size: 10676
BspHI T'CATG_A
Cuts at: 9852 9852
Size: 10676
BspMI ACCTGCnnnn'nnnn_
Cuts at: 218 785 1577 2415 2505 2618 2701 2912 3275
Size: 567 792 838 90 113 83 211 363
Cuts at: 3275 3374 3438 3551 3683 3701 4037 6182 7364
Size: 99 64 113 132 18 336 2145 1182
Cuts at: 7364 7726 7940 8242 8623 218
Size: 362 214 302 381 2271
Fragments arranged by size:
2271 2145 1182 838 792 567 381 363
362 336 302 214 211 132 113 113
99 90 83 64 18
BsrBI CCG'CTC
Cuts at: 50 664 1341 2466 5498 5567 5764 5852 7631
Size: 614 677 1125 3032 69 197 88 1779
Cuts at: 7631 8968 9022 10506 10671 50
Size: 1337 54 1484 165 55
Fragments arranged by size:
3032 1779 1484 1337 1125 677 614 197
165 88 69 55 54
BsrDI GCAATG_nn'
Cuts at: 292 1391 3206 8588 292
Size: 1099 1815 5382 2380
Fragments arranged by size:
5382 2380 1815 1099
BsrFI r'CCGG_y
Cuts at: 877 940 1024 1273 1888 2485 2945 2966 3841
Size: 63 84 249 615 597 460 21 875
Cuts at: 3841 4945 5221 7300 8674 8855 9064 877
Size: 1104 276 2079 1374 181 209 2489
Fragments arranged by size:
2489 2079 1374 1104 875 615 597 460
276 249 209 181 84 63 21
BsrGI T'GTAC_A
Cuts at: 2567 3767 4811 2567
Size: 1200 1044 8432
Fragments arranged by size:
8432 1200 1044
BssHII G'CGCG_C
Cuts at: 158 1385 158
Size: 1227 9449
BssSI C'ACGA_G
Cuts at: 1264 1897 2002 3742 7658 8947 9305 1264
Size: 633 105 1740 3916 1289 358 2635
Fragments arranged by size:
3916 2635 1740 1289 633 358 105
BstAPI GCAn_nnn'nTGC
Cuts at: 357 851 1221 2913 4432 4626 8053 357
Size: 494 370 1692 1519 194 3427 2980
Fragments arranged by size:
3427 2980 1692 1519 494 370 194
BstBI TT'CG_AA
Cuts at: 9037 9037
Size: 10676
BstEII G'GTnAC_C
Cuts at: 1758 3150 4461 1758
Size: 1392 1311 7973
Fragments arranged by size:
7973 1392 1311
BstXI CCAn_nnnn'nTGG
Cuts at: 468 1593 2160 4887 7452 468
Size: 1125 567 2727 2565 3692
Fragments arranged by size:
3692 2727 2565 1125 567
BstYI r'GATC_y
Cuts at: 1 341 1847 3039 3098 6347 7490 7701 8168
Size: 340 1506 1192 59 3249 1143 211 467
Cuts at: 8168 8191 8526 8772 9784 9870 9882 10228 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
3249 1506 1192 1143 1012 467 449 346
340 335 246 211 86 59 23 12
Bsu36I CC'TnA_GG
Cuts at: 6893 6893
Size: 10676
BtgI C'CryG_G
Cuts at: 107 562 857 1082 1562 1763 2663 2801 2837
Size: 455 295 225 480 201 900 138 36
Cuts at: 2837 4300 4713 5657 5825 6149 6704 7364 107
Size: 1463 413 944 168 324 555 660 3419
Fragments arranged by size:
3419 1463 944 900 660 555 480 455
413 324 295 225 201 168 138 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 729 2016 6642 8626 8807 8970 729
Size: 1287 4626 1984 181 163 2435
Fragments arranged by size:
4626 2435 1984 1287 181 163
BtsI GCAGTG_nn'
Cuts at: 724 2303 2311 3448 3555 4315 4968 4974 6473
Size: 1579 8 1137 107 760 653 6 1499
Cuts at: 6473 7103 7919 724
Size: 630 816 3481
Fragments arranged by size:
3481 1579 1499 1137 816 760 653 630
107 8 6
ClaI AT'CG_AT
Cuts at: 6731 6731
Size: 10676
DraI TTT'AAA
Cuts at: 7675 7675
Size: 10676
DraIII CAC_nnn'GTG
Cuts at: 806 1221 2687 4432 5382 806
Size: 415 1466 1745 950 6100
Fragments arranged by size:
6100 1745 1466 950 415
DrdI GACnn_nn'nnGTC
Cuts at: 8382 9240 8382
Size: 858 9818
EaeI y'GGCC_r
Cuts at: 659 716 879 1122 1275 1493 1890 2402 3113
Size: 57 163 243 153 218 397 512 711
Cuts at: 3113 3176 3587 5039 5156 5303 5612 5873 5926
Size: 63 411 1452 117 147 309 261 53
Cuts at: 5926 6068 6224 6863 6995 7121 8261 8435 8826
Size: 142 156 639 132 126 1140 174 391
Cuts at: 8826 8853 10198 659
Size: 27 1345 1137
Fragments arranged by size:
1452 1345 1140 1137 711 639 512 411
397 391 309 261 243 218 174 163
156 153 147 142 132 126 117 63
57 53 27
EagI C'GGCC_G
Cuts at: 1890 5873 5926 6995 8261 1890
Size: 3983 53 1069 1266 4305
Fragments arranged by size:
4305 3983 1266 1069 53
EarI CTCTTCn'nnn_
Cuts at: 220 319 790 1933 1954 2152 3241 3706 3871
Size: 99 471 1143 21 198 1089 465 165
Cuts at: 3871 4382 5792 5804 5833 5856 6421 6427 6655
Size: 511 1410 12 29 23 565 6 228
Cuts at: 6655 6688 7177 8699 8909 220
Size: 33 489 1522 210 1987
Fragments arranged by size:
1987 1522 1410 1143 1089 565 511 489
471 465 228 210 198 165 99 33
29 23 21 12 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1989 5208 6327 8884 9194 9340 10486 1989
Size: 3219 1119 2557 310 146 1146 2179
Fragments arranged by size:
3219 2557 2179 1146 1119 310 146
EcoICRI GAG'CTC
Cuts at: 1283 2145 4767 5688 6981 7688 1283
Size: 862 2622 921 1293 707 4271
Fragments arranged by size:
4271 2622 1293 921 862 707
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 322 463 750 825 981 1186 1645 1724
Size: 151 141 287 75 156 205 459 79
Cuts at: 1724 1744 2136 2143 2364 2453 2494 2557 2608
Size: 20 392 7 221 89 41 63 51
Cuts at: 2608 3115 3339 3532 3601 3852 4033 4056 4282
Size: 507 224 193 69 251 181 23 226
Cuts at: 4282 4427 5104 5455 5876 7467 7606 7612 8501
Size: 145 677 351 421 1591 139 6 889
Cuts at: 8501 8933 9680 9948 171
Size: 432 747 268 899
Fragments arranged by size:
1591 899 889 747 677 507 459 432
421 392 351 287 268 251 226 224
221 205 193 181 156 151 145 141
139 89 79 75 69 63 51 41
23 20 7 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1951 2590 4759 5911 6424 7042 7132 1951
Size: 639 2169 1152 513 618 90 5495
Fragments arranged by size:
5495 2169 1152 639 618 513 90
EcoO109I rG'GnC_Cy
Cuts at: 382 711 982 1597 2112 2216 2306 2451 2833
Size: 329 271 615 515 104 90 145 382
Cuts at: 2833 3384 3563 4243 4251 4270 4581 4582 4843
Size: 551 179 680 8 19 311 1 261
Cuts at: 4843 4854 5666 5693 5745 5820 7035 7815 382
Size: 11 812 27 52 75 1215 780 3243
Fragments arranged by size:
3243 1215 812 780 680 615 551 515
382 329 311 271 261 179 145 104
90 75 52 27 19 11 8 1
EcoRI G'AATT_C
Cuts at: 7680 7680
Size: 10676
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5427 6219 7635 8695 5427
Size: 792 1416 1060 7408
Fragments arranged by size:
7408 1416 1060 792
FspI TGC'GCA
Cuts at: 1096 1477 5071 6178 8457 1096
Size: 381 3594 1107 2279 3315
Fragments arranged by size:
3594 3315 2279 1107 381
HaeII r_GCGC'y
Cuts at: 531 878 2952 3247 3318 4005 4669 4749 5067
Size: 347 2074 295 71 687 664 80 318
Cuts at: 5067 5125 5292 5394 5940 6217 6788 6937 8110
Size: 58 167 102 546 277 571 149 1173
Cuts at: 8110 8358 9380 10359 531
Size: 248 1022 979 848
Fragments arranged by size:
2074 1173 1022 979 848 687 664 571
546 347 318 295 277 248 167 149
102 80 71 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 245 277 543 575 2233 2265 2488 2520 4126
Size: 32 266 32 1658 32 223 32 1606
Cuts at: 4126 4158 5530 5562 6067 6099 6820 6852 8518
Size: 32 1372 32 505 32 721 32 1666
Cuts at: 8518 8548 8550 8580 245
Size: 30 2 30 2341
Fragments arranged by size:
2341 1666 1658 1606 1372 721 505 266
223 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2739 3498 6018 6159 6720 7715 2739
Size: 759 2520 141 561 995 5700
Fragments arranged by size:
5700 2520 995 759 561 141
KasI G'GCGC_C
Cuts at: 527 874 2948 5063 8106 8354 527
Size: 347 2074 2115 3043 248 2849
Fragments arranged by size:
3043 2849 2115 2074 347 248
KpnI G_GTAC'C
Cuts at: 6109 7696 6109
Size: 1587 9089
MluI A'CGCG_T
Cuts at: 2508 10109 2508
Size: 7601 3075
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4400 5631 9347 9531 4400
Size: 1231 3716 184 5545
Fragments arranged by size:
5545 3716 1231 184
MscI TGG'CCA
Cuts at: 1495 2404 3178 6226 6865 7123 8437 1495
Size: 909 774 3048 639 258 1314 3734
Fragments arranged by size:
3734 3048 1314 909 774 639 258
MslI CAynn'nnrTG
Cuts at: 493 1396 2161 2236 2425 2704 5635 8665 8792
Size: 903 765 75 189 279 2931 3030 127
Cuts at: 8792 493
Size: 2377
Fragments arranged by size:
3030 2931 2377 903 765 279 189 127
75
MspA1I CmG'CkG
Cuts at: 191 389 398 859 1427 1617 1744 2106 2199
Size: 198 9 461 568 190 127 362 93
Cuts at: 2199 2979 2988 3014 3030 3460 3519 4155 4443
Size: 780 9 26 16 430 59 636 288
Cuts at: 4443 4597 4809 5505 6353 6512 6678 6771 6861
Size: 154 212 696 848 159 166 93 90
Cuts at: 6861 7119 7227 7272 7787 8101 8461 9474 9719
Size: 258 108 45 515 314 360 1013 245
Cuts at: 9719 191
Size: 1148
Fragments arranged by size:
1148 1013 848 780 696 636 568 515
461 430 362 360 314 288 258 245
212 198 190 166 159 154 127 108
93 93 90 59 45 26 16 9
9
NaeI GCC'GGC
Cuts at: 879 942 1890 2487 2968 5223 8857 879
Size: 63 948 597 481 2255 3634 2698
Fragments arranged by size:
3634 2698 2255 948 597 481 63
NarI GG'CG_CC
Cuts at: 528 875 2949 5064 8107 8355 528
Size: 347 2074 2115 3043 248 2849
Fragments arranged by size:
3043 2849 2115 2074 347 248
NcoI C'CATG_G
Cuts at: 107 1562 2663 6149 7364 107
Size: 1455 1101 3486 1215 3419
Fragments arranged by size:
3486 3419 1455 1215 1101
NgoMIV G'CCGG_C
Cuts at: 877 940 1888 2485 2966 5221 8855 877
Size: 63 948 597 481 2255 3634 2698
Fragments arranged by size:
3634 2698 2255 948 597 481 63
NheI G'CTAG_C
Cuts at: 1170 4065 1170
Size: 2895 7781
NruI TCG'CGA
Cuts at: 414 414
Size: 10676
NspI r_CATG'y
Cuts at: 1869 1923 3579 3801 7083 7729 8229 9136 1869
Size: 54 1656 222 3282 646 500 907 3409
Fragments arranged by size:
3409 3282 1656 907 646 500 222 54
PasI CC'CwG_GG
Cuts at: 1593 4239 5913 1593
Size: 2646 1674 6356
Fragments arranged by size:
6356 2646 1674
PciI A'CATG_T
Cuts at: 1919 9132 1919
Size: 7213 3463
PflMI CCAn_nnn'nTGG
Cuts at: 378 1011 1218 1350 1491 1844 2687 3030 4566
Size: 633 207 132 141 353 843 343 1536
Cuts at: 4566 5154 6288 6668 6861 7155 378
Size: 588 1134 380 193 294 3899
Fragments arranged by size:
3899 1536 1134 843 633 588 380 353
343 294 207 193 141 132
PfoI T'CCnGG_A
Cuts at: 2054 5054 2054
Size: 3000 7676
PmeI GTTT'AAAC
Cuts at: 7675 7675
Size: 10676
PmlI CAC'GTG
Cuts at: 120 1218 2247 5379 120
Size: 1098 1029 3132 5417
Fragments arranged by size:
5417 3132 1098 1029
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6259 6291 6757 6789 9815 9847 6259
Size: 32 466 32 3026 32 7088
Fragments arranged by size:
7088 3026 466 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 382 711 982 2112 2306 3563 4251 5666 7035
Size: 329 271 1130 194 1257 688 1415 1369
Cuts at: 7035 382
Size: 4023
Fragments arranged by size:
4023 1415 1369 1257 1130 688 329 271
194
PshAI GACnn'nnGTC
Cuts at: 1756 2695 4220 1756
Size: 939 1525 8212
Fragments arranged by size:
8212 1525 939
PspOMI G'GGCC_C
Cuts at: 1596 2833 3383 3734 4242 4270 4581 5745 7201
Size: 1237 550 351 508 28 311 1164 1456
Cuts at: 7201 1596
Size: 5071
Fragments arranged by size:
5071 1456 1237 1164 550 508 351 311
28
PspXI vC'TCGA_Gb
Cuts at: 7132 7132
Size: 10676
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5392 5424 5392
Size: 32 10644
PstI C_TGCA'G
Cuts at: 394 620 2198 3562 3722 4001 4697 4775 5891
Size: 226 1578 1364 160 279 696 78 1116
Cuts at: 5891 7232 7334 7723 8408 394
Size: 1341 102 389 685 2662
Fragments arranged by size:
2662 1578 1364 1341 1116 696 685 389
279 226 160 102 78
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10676
PvuII CAG'CTG
Cuts at: 389 1427 1617 1744 2106 2199 2979 3030 3460
Size: 1038 190 127 362 93 780 51 430
Cuts at: 3460 4155 4443 4809 5505 6512 7119 7227 7272
Size: 695 288 366 696 1007 607 108 45
Cuts at: 7272 8101 8461 389
Size: 829 360 2604
Fragments arranged by size:
2604 1038 1007 829 780 696 695 607
430 366 362 360 288 190 127 108
93 51 45
RsrII CG'GwC_CG
Cuts at: 7343 8871 7343
Size: 1528 9148
SacI G_AGCT'C
Cuts at: 1285 2147 4769 5690 6983 7690 1285
Size: 862 2622 921 1293 707 4271
Fragments arranged by size:
4271 2622 1293 921 862 707
SacII CC_GC'GG
Cuts at: 860 860
Size: 10676
SalI G'TCGA_C
Cuts at: 6016 7713 6016
Size: 1697 8979
SanDI GG'GwC_CC
Cuts at: 382 982 2306 5666 382
Size: 600 1324 3360 5392
Fragments arranged by size:
5392 3360 1324 600
SapI GCTCTTCn'nnn_
Cuts at: 220 319 790 2152 3706 3871 6688 8699 8909
Size: 99 471 1362 1554 165 2817 2011 210
Cuts at: 8909 220
Size: 1987
Fragments arranged by size:
2817 2011 1987 1554 1362 471 210 165
99
SbfI CC_TGCA'GG
Cuts at: 4775 7723 4775
Size: 2948 7728
ScaI AGT'ACT
Cuts at: 1843 3061 10193 1843
Size: 1218 7132 2326
Fragments arranged by size:
7132 2326 1218
SexAI A'CCwGG_T
Cuts at: 802 5465 6101 802
Size: 4663 636 5377
Fragments arranged by size:
5377 4663 636
SfcI C'TryA_G
Cuts at: 33 390 616 1962 2194 3558 3718 3997 4693
Size: 357 226 1346 232 1364 160 279 696
Cuts at: 4693 4771 5887 7228 7330 7719 8404 9397 9588
Size: 78 1116 1341 102 389 685 993 191
Cuts at: 9588 33
Size: 1121
Fragments arranged by size:
1364 1346 1341 1121 1116 993 696 685
389 357 279 232 226 191 160 102
78
SfiI GGCCn_nnn'nGGCC
Cuts at: 1305 1305
Size: 10676
SfoI GGC'GCC
Cuts at: 529 876 2950 5065 8108 8356 529
Size: 347 2074 2115 3043 248 2849
Fragments arranged by size:
3043 2849 2115 2074 347 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10676
SmaI CCC'GGG
Cuts at: 1326 4992 7698 1326
Size: 3666 2706 4304
Fragments arranged by size:
4304 3666 2706
SmlI C'TyrA_G
Cuts at: 967 1984 2728 3055 4105 6748 7132 7540 7831
Size: 1017 744 327 1050 2643 384 408 291
Cuts at: 7831 8746 9238 9500 967
Size: 915 492 262 2143
Fragments arranged by size:
2643 2143 1050 1017 915 744 492 408
384 327 291 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 10676
SphI G_CATG'C
Cuts at: 7729 8229 7729
Size: 500 10176
StuI AGG'CCT
Cuts at: 1990 6823 1990
Size: 4833 5843
StyI C'CwwG_G
Cuts at: 107 264 914 1562 1653 2663 3905 4247 5549
Size: 157 650 648 91 1010 1242 342 1302
Cuts at: 5549 6149 7364 7810 8164 107
Size: 600 1215 446 354 2619
Fragments arranged by size:
2619 1302 1242 1215 1010 650 648 600
446 354 342 157 91
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2568 4449 6468 6622 7360 8259 8392 8851 9060
Size: 1881 2019 154 738 899 133 459 209
Cuts at: 9060 2568
Size: 4184
Fragments arranged by size:
4184 2019 1881 899 738 459 209 154
133
TatI w'GTAC_w
Cuts at: 137 1841 2567 3059 3767 4811 6398 6884 7181
Size: 1704 726 492 708 1044 1587 486 297
Cuts at: 7181 10191 137
Size: 3010 622
Fragments arranged by size:
3010 1704 1587 1044 726 708 622 492
486 297
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3547 3579 4751 4783 6466 6498 7040 7072 7507
Size: 32 1172 32 1683 32 542 32 435
Cuts at: 7507 7539 10193 10225 3547
Size: 32 2654 32 3998
Fragments arranged by size:
3998 2654 1683 1172 542 435 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3973 6901 7384 8473 3973
Size: 2928 483 1089 6176
Fragments arranged by size:
6176 2928 1089 483
XbaI T'CTAG_A
Cuts at: 7707 7707
Size: 10676
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 468 2451 3912 4307 4563 4960 8171 468
Size: 1983 1461 395 256 397 3211 2973
Fragments arranged by size:
3211 2973 1983 1461 397 395 256
XhoI C'TCGA_G
Cuts at: 7132 7132
Size: 10676
XmaI C'CCGG_G
Cuts at: 1324 4990 7696 1324
Size: 3666 2706 4304
Fragments arranged by size:
4304 3666 2706
XmnI GAAnn'nnTTC
Cuts at: 2872 2872
Size: 10676
ZraI GAC'GTC
Cuts at: 2499 5985 2499
Size: 3486 7190
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NruI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AflII AloI AscI AvrII BaeI BfrBI BsiWI BsmI
BstZ17I CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NotI NsiI PacI PsiI SgrAI SnaBI SrfI
SspI SwaI