Restriction map for
pF1KA0234
(Circular) (Six-base) MAPSORT of: pF1KA0234.seq Check: 2837 from: 1 to: 7559
pF1KA0234 7559 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2658 2951 3462 2658
Size: 293 511 6755
Fragments arranged by size:
6755 511 293
AatII G_ACGT'C
Cuts at: 1944 1944
Size: 7559
AccI GT'mk_AC
Cuts at: 567 1944 3875 4597 567
Size: 1377 1931 722 3529
Fragments arranged by size:
3529 1931 1377 722
Acc65I G'GTAC_C
Cuts at: 4575 4575
Size: 7559
AclI AA'CG_TT
Cuts at: 4295 7399 4295
Size: 3104 4455
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1067 1763 2215 2326 2371 2644 2666 3715 3812
Size: 696 452 111 45 273 22 1049 97
Cuts at: 3812 3866 4306 4475 5384 5816 6169 6831 1067
Size: 54 440 169 909 432 353 662 1795
Fragments arranged by size:
1795 1049 909 696 662 452 440 432
353 273 169 111 97 54 45 22
AfeI AGC'GCT
Cuts at: 2842 2842
Size: 7559
AflIII A'CryG_T
Cuts at: 776 4911 6711 6992 776
Size: 4135 1800 281 1343
Fragments arranged by size:
4135 1800 1343 281
AleI CACnn'nnGTG
Cuts at: 144 857 144
Size: 713 6846
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1417 3785 1417
Size: 2368 5191
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2444 2476 2444
Size: 32 7527
AlwNI CAG_nnn'CTG
Cuts at: 1900 2349 2628 2709 3220 3331 3699 3889 4145
Size: 449 279 81 511 111 368 190 256
Cuts at: 4145 4155 4864 6302 1900
Size: 10 709 1438 3157
Fragments arranged by size:
3157 1438 709 511 449 368 279 256
190 111 81 10
ApaLI G'TGCA_C
Cuts at: 1903 6397 1903
Size: 4494 3065
ApoI r'AATT_y
Cuts at: 175 379 389 1177 1249 3174 3619 4187 4213
Size: 204 10 788 72 1925 445 568 26
Cuts at: 4213 4563 6912 7168 175
Size: 350 2349 256 566
Fragments arranged by size:
2349 1925 788 568 566 445 350 256
204 72 26 10
AseI AT'TA_AT
Cuts at: 20 20
Size: 7559
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7559
AvaI C'yCGr_G
Cuts at: 2804 4579 2804
Size: 1775 5784
BamHI G'GATC_C
Cuts at: 4584 4584
Size: 7559
BanI G'GyrC_C
Cuts at: 1007 1152 1401 2640 2767 3423 3474 4575 4989
Size: 145 249 1239 127 656 51 1101 414
Cuts at: 4989 5237 5272 1007
Size: 248 35 3294
Fragments arranged by size:
3294 1239 1101 656 414 249 248 145
127 51 35
BanII G_rGCy'C
Cuts at: 818 1847 2174 2734 4003 4080 4094 4251 4338
Size: 1029 327 560 1269 77 14 157 87
Cuts at: 4338 4345 4573 5603 818
Size: 7 228 1030 2774
Fragments arranged by size:
2774 1269 1030 1029 560 327 228 157
87 77 14 7
BbeI G_GCGC'C
Cuts at: 4993 5241 4993
Size: 248 7311
BbsI GAAGACnn'nnnn_
Cuts at: 1214 1507 1821 1214
Size: 293 314 6952
Fragments arranged by size:
6952 314 293
BbvCI CC'TCA_GC
Cuts at: 2552 2552
Size: 7559
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3373 3407 3373
Size: 34 7525
BciVI GTATCCnnnnn_n'
Cuts at: 3388 5457 6513 7559 3388
Size: 2069 1056 1046 3388
Fragments arranged by size:
3388 2069 1056 1046
BclI T'GATC_A
Cuts at: 5079 5079
Size: 7559
BfrBI ATG'CAT
Cuts at: 2499 2499
Size: 7559
BglI GCCn_nnn'nGGC
Cuts at: 2084 3087 3708 3778 2084
Size: 1003 621 70 5865
Fragments arranged by size:
5865 1003 621 70
BglII A'GATC_T
Cuts at: 431 5074 7111 431
Size: 4643 2037 879
Fragments arranged by size:
4643 2037 879
BlpI GC'TnA_GC
Cuts at: 1982 2810 3824 4671 1982
Size: 828 1014 847 4870
Fragments arranged by size:
4870 1014 847 828
Bme1580I G_kGCm'C
Cuts at: 151 864 1012 1406 1907 2295 3548 5184 5277
Size: 713 148 394 501 388 1253 1636 93
Cuts at: 5277 6401 151
Size: 1124 1309
Fragments arranged by size:
1636 1309 1253 1124 713 501 394 388
148 93
BmgBI CAC'GTC
Cuts at: 1212 1212
Size: 7559
BmrI ACTGGGnnnn_n'
Cuts at: 858 3407 3566 4771 4852 5187 7331 858
Size: 2549 159 1205 81 335 2144 1086
Fragments arranged by size:
2549 2144 1205 1086 335 159 81
BmtI G_CTAG'C
Cuts at: 3598 3598
Size: 7559
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3168 3885 4265 5926 3168
Size: 717 380 1661 4801
Fragments arranged by size:
4801 1661 717 380
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 1023 1203 1482 2317 2766 2890 3159 3970
Size: 671 180 279 835 449 124 269 811
Cuts at: 3970 3974 4030 4105 4157 4222 4273 352
Size: 4 56 75 52 65 51 3638
Fragments arranged by size:
3638 835 811 671 449 279 269 180
124 75 65 56 52 51 4
Bpu10I CC'TnA_GC
Cuts at: 692 1568 2287 2552 4344 692
Size: 876 719 265 1792 3907
Fragments arranged by size:
3907 1792 876 719 265
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 778 825 2372 2488 3151 3214 4135 4735 5614
Size: 47 1547 116 663 63 921 600 879
Cuts at: 5614 6328 6626 778
Size: 714 298 1711
Fragments arranged by size:
1711 1547 921 879 714 663 600 298
116 63 47
BsaI GGTCTCn'nnnn_
Cuts at: 3314 7263 3314
Size: 3949 3610
BsaAI yAC'GTr
Cuts at: 1416 2045 4912 1416
Size: 629 2867 4063
Fragments arranged by size:
4063 2867 629
BsaBI GATnn'nnATC
Cuts at: 6 1886 5073 7110 6
Size: 1880 3187 2037 455
Fragments arranged by size:
3187 2037 1880 455
BsaHI Gr'CG_yC
Cuts at: 1785 1941 1970 4990 5238 5940 7436 1785
Size: 156 29 3020 248 702 1496 1908
Fragments arranged by size:
3020 1908 1496 702 248 156 29
BsaWI w'CCGG_w
Cuts at: 141 2147 4748 5269 5947 6358 6505 6779 141
Size: 2006 2601 521 678 411 147 274 921
Fragments arranged by size:
2601 2006 921 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1294 1324 2129 2159 3570 3600 3950 3980 4003
Size: 30 805 30 1411 30 350 30 23
Cuts at: 4003 4033 4309 4339 1294
Size: 30 276 30 4514
Fragments arranged by size:
4514 1411 805 350 276 30 30 30
30 30 30 23
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 296 390 622 1315 1558 1855 1948 2331 3544
Size: 94 232 693 243 297 93 383 1213
Cuts at: 3544 3877 4102 296
Size: 333 225 3753
Fragments arranged by size:
3753 1213 693 383 333 297 243 232
225 94 93
BseYI C'CCAG_C
Cuts at: 167 2628 2843 3264 3294 3481 4984 5742 6407
Size: 2461 215 421 30 187 1503 758 665
Cuts at: 6407 6815 167
Size: 408 911
Fragments arranged by size:
2461 1503 911 758 665 421 408 215
187 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 559 804 1694 3233 3290 559
Size: 245 890 1539 57 4828
Fragments arranged by size:
4828 1539 890 245 57
BsiEI CG_ry'CG
Cuts at: 104 5101 5147 6377 104
Size: 4997 46 1230 1286
Fragments arranged by size:
4997 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1847 1907 2128 2174 4094 4345 4573 5351 5541
Size: 60 221 46 1920 251 228 778 190
Cuts at: 5541 6401 1847
Size: 860 3005
Fragments arranged by size:
3005 1920 860 778 251 228 221 190
60 46
BsmBI CGTCTCn'nnnn_
Cuts at: 1867 1867
Size: 7559
Bsp1286I G_dGCh'C
Cuts at: 151 818 864 1012 1406 1847 1907 2128 2174
Size: 667 46 148 394 441 60 221 46
Cuts at: 2174 2295 2734 3548 4003 4080 4094 4251 4338
Size: 121 439 814 455 77 14 157 87
Cuts at: 4338 4345 4573 5184 5277 5351 5541 5603 6401
Size: 7 228 611 93 74 190 62 798
Cuts at: 6401 151
Size: 1309
Fragments arranged by size:
1309 814 798 667 611 455 441 439
394 228 221 190 157 148 121 93
87 77 74 62 60 46 46 14
7
BspHI T'CATG_A
Cuts at: 1616 4094 5991 1616
Size: 2478 1897 3184
Fragments arranged by size:
3184 2478 1897
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1785 2658 2951 3086 3108 3462 4118 4609 4823
Size: 873 293 135 22 354 656 491 214
Cuts at: 4823 5125 5506 1785
Size: 302 381 3838
Fragments arranged by size:
3838 873 656 491 381 354 302 293
214 135 22
BsrBI CCG'CTC
Cuts at: 50 426 5851 5905 7389 7554 50
Size: 376 5425 54 1484 165 55
Fragments arranged by size:
5425 1484 376 165 55 54
BsrDI GCAATG_nn'
Cuts at: 197 466 787 2689 2831 5471 197
Size: 269 321 1902 142 2640 2285
Fragments arranged by size:
2640 2285 1902 321 269 142
BsrFI r'CCGG_y
Cuts at: 1808 3245 5557 5738 1808
Size: 1437 2312 181 3629
Fragments arranged by size:
3629 2312 1437 181
BsrGI T'GTAC_A
Cuts at: 1143 1143
Size: 7559
BssSI C'ACGA_G
Cuts at: 1700 1837 2309 2558 5830 6538 1700
Size: 137 472 249 3272 708 2721
Fragments arranged by size:
3272 2721 708 472 249 137
BstAPI GCAn_nnn'nTGC
Cuts at: 2033 3699 4155 4936 2033
Size: 1666 456 781 4656
Fragments arranged by size:
4656 1666 781 456
BstBI TT'CG_AA
Cuts at: 843 5920 843
Size: 5077 2482
BstEII G'GTnAC_C
Cuts at: 3022 4119 3022
Size: 1097 6462
BstXI CCAn_nnnn'nTGG
Cuts at: 672 3420 672
Size: 2748 4811
BstYI r'GATC_y
Cuts at: 1 431 1881 3011 4584 5051 5074 5409 5655
Size: 430 1450 1130 1573 467 23 335 246
Cuts at: 5655 5961 5973 6059 7111 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1573 1450 1130 1052 467 449 430 335
306 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1298 3716 4064 1298
Size: 2418 348 4793
Fragments arranged by size:
4793 2418 348
BtgI C'CryG_G
Cuts at: 107 254 900 1641 3525 107
Size: 147 646 741 1884 4141
Fragments arranged by size:
4141 1884 741 646 147
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5509 5690 5853 5509
Size: 181 163 7215
Fragments arranged by size:
7215 181 163
BtsI GCAGTG_nn'
Cuts at: 864 1263 2575 3187 3505 3538 3589 3887 3921
Size: 399 1312 612 318 33 51 298 34
Cuts at: 3921 4802 864
Size: 881 3621
Fragments arranged by size:
3621 1312 881 612 399 318 298 51
34 33
DraI TTT'AAA
Cuts at: 407 4558 407
Size: 4151 3408
DrdI GACnn_nn'nnGTC
Cuts at: 5265 6609 5265
Size: 1344 6215
EaeI y'GGCC_r
Cuts at: 2262 2417 2570 3652 5144 5318 5709 5736 7081
Size: 155 153 1082 1492 174 391 27 1345
Cuts at: 7081 2262
Size: 2740
Fragments arranged by size:
2740 1492 1345 1082 391 174 155 153
27
EagI C'GGCC_G
Cuts at: 5144 5144
Size: 7559
EarI CTCTTCn'nnn_
Cuts at: 2276 3089 3447 4494 5582 5792 2276
Size: 813 358 1047 1088 210 4043
Fragments arranged by size:
4043 1088 1047 813 358 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 235 1042 5767 6509 6655 7369 235
Size: 807 4725 742 146 714 425
Fragments arranged by size:
4725 807 742 714 425 146
EcoICRI GAG'CTC
Cuts at: 1845 2172 4092 4343 4571 1845
Size: 327 1920 251 228 4833
Fragments arranged by size:
4833 1920 327 251 228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 1023 1067 1203 1482 1763 2215 2317 2326
Size: 671 44 136 279 281 452 102 9
Cuts at: 2326 2371 2644 2666 2766 2890 3159 3715 3812
Size: 45 273 22 100 124 269 556 97
Cuts at: 3812 3866 3970 3974 4030 4105 4157 4222 4273
Size: 54 104 4 56 75 52 65 51
Cuts at: 4273 4306 4475 5384 5816 6169 6831 352
Size: 33 169 909 432 353 662 1080
Fragments arranged by size:
1080 909 671 662 556 452 432 353
281 279 273 269 169 136 124 104
102 100 97 75 65 56 54 52
51 45 44 33 22 9 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2365 2530 3511 4114 2365
Size: 165 981 603 5810
Fragments arranged by size:
5810 981 603 165
EcoO109I rG'GnC_Cy
Cuts at: 661 1953 2460 2706 3054 3075 3312 3726 4107
Size: 1292 507 246 348 21 237 414 381
Cuts at: 4107 4698 661
Size: 591 3522
Fragments arranged by size:
3522 1292 591 507 414 381 348 246
237 21
EcoRI G'AATT_C
Cuts at: 175 1177 3174 4187 4563 175
Size: 1002 1997 1013 376 3171
Fragments arranged by size:
3171 1997 1013 1002 376
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 205 819 5578 205
Size: 614 4759 2186
Fragments arranged by size:
4759 2186 614
FspI TGC'GCA
Cuts at: 5340 5340
Size: 7559
HaeII r_GCGC'y
Cuts at: 1821 2844 3265 4993 5241 6471 7242 1821
Size: 1023 421 1728 248 1230 771 2138
Fragments arranged by size:
2138 1728 1230 1023 771 421 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1866 1898 2129 2161 2415 2447 3208 3240 3313
Size: 32 231 32 254 32 761 32 73
Cuts at: 3313 3345 4003 4035 4309 4341 4373 4471 4503
Size: 32 658 32 274 32 32 98 32
Cuts at: 4503 5401 5431 5433 5463 1866
Size: 898 30 2 30 3962
Fragments arranged by size:
3962 898 761 658 274 254 231 98
73 32 32 32 32 32 32 32
32 32 30 30 2
HincII GTy'rAC
Cuts at: 288 568 1752 4040 4598 288
Size: 280 1184 2288 558 3249
Fragments arranged by size:
3249 2288 1184 558 280
HindIII A'AGCT_T
Cuts at: 919 1049 2987 919
Size: 130 1938 5491
Fragments arranged by size:
5491 1938 130
KasI G'GCGC_C
Cuts at: 4989 5237 4989
Size: 248 7311
KpnI G_GTAC'C
Cuts at: 4579 4579
Size: 7559
MluI A'CGCG_T
Cuts at: 6992 6992
Size: 7559
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1148 1306 1859 2591 3472 4034 4213 6318 6502
Size: 158 553 732 881 562 179 2105 184
Cuts at: 6502 1148
Size: 2205
Fragments arranged by size:
2205 2105 881 732 562 553 184 179
158
MscI TGG'CCA
Cuts at: 2419 2572 5320 2419
Size: 153 2748 4658
Fragments arranged by size:
4658 2748 153
MslI CAynn'nnrTG
Cuts at: 144 857 1376 1399 1490 1615 1633 2108 5548
Size: 713 519 23 91 125 18 475 3440
Cuts at: 5548 5675 144
Size: 127 2028
Fragments arranged by size:
3440 2028 713 519 475 127 125 91
23 18
MspA1I CmG'CkG
Cuts at: 256 2439 2628 3387 3481 3819 4152 4495 4670
Size: 2183 189 759 94 338 333 343 175
Cuts at: 4670 4984 5344 6128 6373 256
Size: 314 360 784 245 1442
Fragments arranged by size:
2183 1442 784 759 360 343 338 333
314 245 189 175 94
NaeI GCC'GGC
Cuts at: 1810 5740 1810
Size: 3930 3629
NarI GG'CG_CC
Cuts at: 4990 5238 4990
Size: 248 7311
NcoI C'CATG_G
Cuts at: 107 1641 3525 107
Size: 1534 1884 4141
Fragments arranged by size:
4141 1884 1534
NdeI CA'TA_TG
Cuts at: 1148 1148
Size: 7559
NgoMIV G'CCGG_C
Cuts at: 1808 5738 1808
Size: 3930 3629
NheI G'CTAG_C
Cuts at: 3594 3594
Size: 7559
NsiI A_TGCA'T
Cuts at: 2501 2501
Size: 7559
NspI r_CATG'y
Cuts at: 731 1140 4612 5112 6715 731
Size: 409 3472 500 1603 1575
Fragments arranged by size:
3472 1603 1575 500 409
PciI A'CATG_T
Cuts at: 6711 6711
Size: 7559
PflMI CCAn_nnn'nTGG
Cuts at: 1325 3758 1325
Size: 2433 5126
PfoI T'CCnGG_A
Cuts at: 2525 3136 3990 2525
Size: 611 854 6094
Fragments arranged by size:
6094 854 611
PmeI GTTT'AAAC
Cuts at: 4558 4558
Size: 7559
PmlI CAC'GTG
Cuts at: 2045 2045
Size: 7559
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6005 6037 6005
Size: 32 7527
PpuMI rG'GwC_Cy
Cuts at: 661 2460 3075 3312 4107 661
Size: 1799 615 237 795 4113
Fragments arranged by size:
4113 1799 795 615 237
PshAI GACnn'nnGTC
Cuts at: 3509 3509
Size: 7559
PstI C_TGCA'G
Cuts at: 3119 3386 3500 3695 4160 4606 5291 3119
Size: 267 114 195 465 446 685 5387
Fragments arranged by size:
5387 685 465 446 267 195 114
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7559
PvuII CAG'CTG
Cuts at: 2439 2628 3387 3481 3819 4152 4495 4984 5344
Size: 189 759 94 338 333 343 489 360
Cuts at: 5344 2439
Size: 4654
Fragments arranged by size:
4654 759 489 360 343 338 333 189
94
RsrII CG'GwC_CG
Cuts at: 5754 5754
Size: 7559
SacI G_AGCT'C
Cuts at: 1847 2174 4094 4345 4573 1847
Size: 327 1920 251 228 4833
Fragments arranged by size:
4833 1920 327 251 228
SacII CC_GC'GG
Cuts at: 257 257
Size: 7559
SalI G'TCGA_C
Cuts at: 566 4596 566
Size: 4030 3529
SapI GCTCTTCn'nnn_
Cuts at: 2276 3089 4494 5582 5792 2276
Size: 813 1405 1088 210 4043
Fragments arranged by size:
4043 1405 1088 813 210
SbfI CC_TGCA'GG
Cuts at: 4606 4606
Size: 7559
ScaI AGT'ACT
Cuts at: 7076 7076
Size: 7559
SexAI A'CCwGG_T
Cuts at: 1504 1504
Size: 7559
SfcI C'TryA_G
Cuts at: 33 444 561 583 602 1157 3115 3382 3496
Size: 411 117 22 19 555 1958 267 114
Cuts at: 3496 3691 3841 4156 4602 5287 6255 6446 33
Size: 195 150 315 446 685 968 191 1146
Fragments arranged by size:
1958 1146 968 685 555 446 411 315
267 195 191 150 117 114 22 19
SfoI GGC'GCC
Cuts at: 4991 5239 4991
Size: 248 7311
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7559
SmaI CCC'GGG
Cuts at: 2806 4581 2806
Size: 1775 5784
SmlI C'TyrA_G
Cuts at: 793 804 2387 2467 3166 3193 4114 4714 5629
Size: 11 1583 80 699 27 921 600 915
Cuts at: 5629 6343 6605 793
Size: 714 262 1747
Fragments arranged by size:
1747 1583 921 915 714 699 600 262
80 27 11
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7559
SphI G_CATG'C
Cuts at: 4612 5112 4612
Size: 500 7059
SrfI GCCC'GGGC
Cuts at: 2806 2806
Size: 7559
SspI AAT'ATT
Cuts at: 3927 3927
Size: 7559
StuI AGG'CCT
Cuts at: 2542 3151 3868 2542
Size: 609 717 6233
Fragments arranged by size:
6233 717 609
StyI C'CwwG_G
Cuts at: 107 1641 1725 2162 2246 3525 3939 4693 5047
Size: 1534 84 437 84 1279 414 754 354
Cuts at: 5047 107
Size: 2619
Fragments arranged by size:
2619 1534 1279 754 437 414 354 84
84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2620 2755 5142 5275 5734 5943 2620
Size: 135 2387 133 459 209 4236
Fragments arranged by size:
4236 2387 459 209 135 133
TatI w'GTAC_w
Cuts at: 1079 1143 1759 7074 1079
Size: 64 616 5315 1564
Fragments arranged by size:
5315 1564 616 64
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 103 135 3570 3602 3950 3982 7076 7108 103
Size: 32 3435 32 348 32 3094 32 554
Fragments arranged by size:
3435 3094 554 348 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5356 5356
Size: 7559
XbaI T'CTAG_A
Cuts at: 3039 4590 3039
Size: 1551 6008
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 187 257 2790 3596 3780 5054 187
Size: 70 2533 806 184 1274 2692
Fragments arranged by size:
2692 2533 1274 806 184 70
XmaI C'CCGG_G
Cuts at: 2804 4579 2804
Size: 1775 5784
XmnI GAAnn'nnTTC
Cuts at: 1177 1177
Size: 7559
ZraI GAC'GTC
Cuts at: 1942 1942
Size: 7559
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NsiI NspI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AflII AgeI AhdI ApaI AscI AvrII BaeI BsiWI
BsmI BspEI BssHII BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HpaI MfeI NotI NruI PacI PasI
PsiI PspOMI PspXI PsrI SanDI SfiI SgrAI SnaBI
SwaI XhoI