Restriction map for
pF1KA0244
(Circular) (Six-base) MAPSORT of: pF1KA0244.seq Check: 1057 from: 1 to: 9230
pF1KA0244 9230 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AccI GT'mk_AC
Cuts at: 2746 3703 6083 6268 2746
Size: 957 2380 185 5708
Fragments arranged by size:
5708 2380 957 185
Acc65I G'GTAC_C
Cuts at: 735 2955 6179 6246 735
Size: 2220 3224 67 3719
Fragments arranged by size:
3719 3224 2220 67
AclI AA'CG_TT
Cuts at: 3652 9070 3652
Size: 5418 3812
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 752 761 1492 2441 3962 3966 4180 4342
Size: 490 9 731 949 1521 4 214 162
Cuts at: 4342 4882 5084 5506 7055 7487 8234 8502 262
Size: 540 202 422 1549 432 747 268 990
Fragments arranged by size:
1549 1521 990 949 747 731 540 490
432 422 268 214 202 162 9 4
AfeI AGC'GCT
Cuts at: 3997 3997
Size: 9230
AflII C'TTAA_G
Cuts at: 447 447
Size: 9230
AflIII A'CryG_T
Cuts at: 1959 5416 6582 7686 8663 1959
Size: 3457 1166 1104 977 2526
Fragments arranged by size:
3457 2526 1166 1104 977
AgeI A'CCGG_T
Cuts at: 7618 7618
Size: 9230
AhdI GACnn_n'nnGTC
Cuts at: 3408 3408
Size: 9230
AlwNI CAG_nnn'CTG
Cuts at: 3793 3940 4158 4178 5832 6535 8102 3793
Size: 147 218 20 1654 703 1567 4921
Fragments arranged by size:
4921 1654 1567 703 218 147 20
ApaLI G'TGCA_C
Cuts at: 4217 5359 8000 4217
Size: 1142 2641 5447
Fragments arranged by size:
5447 2641 1142
ApoI r'AATT_y
Cuts at: 468 1446 1725 1868 2482 2591 2622 2946 3613
Size: 978 279 143 614 109 31 324 667
Cuts at: 3613 3766 3911 3953 4070 4592 4830 5428 5754
Size: 153 145 42 117 522 238 598 326
Cuts at: 5754 6234 8583 8839 468
Size: 480 2349 256 859
Fragments arranged by size:
2349 978 859 667 614 598 522 480
326 324 279 256 238 153 145 143
117 109 42 31
AseI AT'TA_AT
Cuts at: 20 20
Size: 9230
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9230
AvaI C'yCGr_G
Cuts at: 6250 6250
Size: 9230
AvrII C'CTAG_G
Cuts at: 5062 5062
Size: 9230
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 667 700 1691 1724 667
Size: 33 991 33 8173
Fragments arranged by size:
8173 991 33 33
BamHI G'GATC_C
Cuts at: 175 264 5058 6255 175
Size: 89 4794 1197 3150
Fragments arranged by size:
4794 3150 1197 89
BanI G'GyrC_C
Cuts at: 735 2955 3483 4057 4531 5792 6179 6246 6660
Size: 2220 528 574 474 1261 387 67 414
Cuts at: 6660 6908 6943 735
Size: 248 35 3022
Fragments arranged by size:
3022 2220 1261 574 528 474 414 387
248 67 35
BanII G_rGCy'C
Cuts at: 6244 7274 6244
Size: 1030 8200
BbeI G_GCGC'C
Cuts at: 5796 6664 6912 5796
Size: 868 248 8114
Fragments arranged by size:
8114 868 248
BbsI GAAGACnn'nnnn_
Cuts at: 604 746 2393 2432 4370 6119 604
Size: 142 1647 39 1938 1749 3715
Fragments arranged by size:
3715 1938 1749 1647 142 39
BbvCI CC'TCA_GC
Cuts at: 1997 4346 1997
Size: 2349 6881
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 5350 5384 5350
Size: 34 9196
BciVI GTATCCnnnnn_n'
Cuts at: 1236 7128 7889 9230 1236
Size: 5892 761 1341 1236
Fragments arranged by size:
5892 1341 1236 761
BclI T'GATC_A
Cuts at: 2214 3129 6750 2214
Size: 915 3621 4694
Fragments arranged by size:
4694 3621 915
BfrBI ATG'CAT
Cuts at: 3580 3580
Size: 9230
BglI GCCn_nnn'nGGC
Cuts at: 4529 5790 4529
Size: 1261 7969
BglII A'GATC_T
Cuts at: 478 4957 6745 8782 478
Size: 4479 1788 2037 926
Fragments arranged by size:
4479 2037 1788 926
BlpI GC'TnA_GC
Cuts at: 253 6342 253
Size: 6089 3141
Bme1580I G_kGCm'C
Cuts at: 4221 4534 5363 6005 6024 6855 6948 8004 4221
Size: 313 829 642 19 831 93 1056 5447
Fragments arranged by size:
5447 1056 831 829 642 313 93 19
BmgBI CAC'GTC
Cuts at: 3670 3670
Size: 9230
BmrI ACTGGGnnnn_n'
Cuts at: 4554 6442 6523 6858 9002 4554
Size: 1888 81 335 2144 4782
Fragments arranged by size:
4782 2144 1888 335 81
BmtI G_CTAG'C
Cuts at: 2668 2668
Size: 9230
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3024 3420 5244 7597 3024
Size: 396 1824 2353 4657
Fragments arranged by size:
4657 2353 1824 396
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 5618 205
Size: 5413 3817
Bpu10I CC'TnA_GC
Cuts at: 1997 4346 1997
Size: 2349 6881
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1135 2348 5285 5515 6406 7285 7777 8075 1135
Size: 1213 2937 230 891 879 492 298 2290
Fragments arranged by size:
2937 2290 1213 891 879 492 298 230
BsaI GGTCTCn'nnnn_
Cuts at: 5036 8934 5036
Size: 3898 5332
BsaAI yAC'GTr
Cuts at: 2034 6583 2034
Size: 4549 4681
BsaBI GATnn'nnATC
Cuts at: 6 263 6744 8781 6
Size: 257 6481 2037 455
Fragments arranged by size:
6481 2037 455 257
BsaHI Gr'CG_yC
Cuts at: 5793 6661 6909 7611 9107 5793
Size: 868 248 702 1496 5916
Fragments arranged by size:
5916 1496 868 702 248
BsaWI w'CCGG_w
Cuts at: 1398 6419 6940 7618 7892 8039 8450 1398
Size: 5021 521 678 274 147 411 2178
Fragments arranged by size:
5021 2178 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1605 1635 3642 3672 1605
Size: 30 2007 30 7163
Fragments arranged by size:
7163 2007 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2214 2660 3409 3637 3640 2214
Size: 446 749 228 3 7804
Fragments arranged by size:
7804 749 446 228 3
BseYI C'CCAG_C
Cuts at: 2010 2254 4454 6655 7413 7990 8486 2010
Size: 244 2200 2201 758 577 496 2754
Fragments arranged by size:
2754 2201 2200 758 577 496 244
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 726 1931 726
Size: 1205 8025
BsiEI CG_ry'CG
Cuts at: 104 6772 6818 8026 104
Size: 6668 46 1208 1308
Fragments arranged by size:
6668 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 541 1348 4221 5177 5363 6244 7022 7212 8004
Size: 807 2873 956 186 881 778 190 792
Cuts at: 8004 541
Size: 1767
Fragments arranged by size:
2873 1767 956 881 807 792 778 190
186
BsmI GAATG_Cn'
Cuts at: 1467 1467
Size: 9230
BsmBI CGTCTCn'nnnn_
Cuts at: 3180 3180
Size: 9230
Bsp1286I G_dGCh'C
Cuts at: 541 1348 4221 4534 5177 5363 6005 6024 6244
Size: 807 2873 313 643 186 642 19 220
Cuts at: 6244 6855 6948 7022 7212 7274 8004 541
Size: 611 93 74 190 62 730 1767
Fragments arranged by size:
2873 1767 807 730 643 642 611 313
220 190 186 93 74 62 19
BspEI T'CCGG_A
Cuts at: 8450 8450
Size: 9230
BspHI T'CATG_A
Cuts at: 858 2793 8406 858
Size: 1935 5613 1682
Fragments arranged by size:
5613 1935 1682
BspMI ACCTGCnnnn'nnnn_
Cuts at: 4017 6280 6494 6796 7177 4017
Size: 2263 214 302 381 6070
Fragments arranged by size:
6070 2263 381 302 214
BsrBI CCG'CTC
Cuts at: 50 7522 7576 9060 9225 50
Size: 7472 54 1484 165 55
Fragments arranged by size:
7472 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 324 408 2021 4231 7142 324
Size: 84 1613 2210 2911 2412
Fragments arranged by size:
2911 2412 2210 1613 84
BsrFI r'CCGG_y
Cuts at: 7228 7409 7618 7228
Size: 181 209 8840
Fragments arranged by size:
8840 209 181
BsrGI T'GTAC_A
Cuts at: 1156 2844 1156
Size: 1688 7542
BssSI C'ACGA_G
Cuts at: 5229 7501 7859 5229
Size: 2272 358 6600
Fragments arranged by size:
6600 2272 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2980 3467 6607 2980
Size: 487 3140 5603
Fragments arranged by size:
5603 3140 487
BstBI TT'CG_AA
Cuts at: 5370 7591 5370
Size: 2221 7009
BstYI r'GATC_y
Cuts at: 1 175 264 277 478 1126 2448 2707 3075
Size: 174 89 13 201 648 1322 259 368
Cuts at: 3075 3376 4957 5058 6255 6722 6745 7080 7326
Size: 301 1581 101 1197 467 23 335 246
Cuts at: 7326 8338 8424 8436 8782 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1581 1322 1197 1012 648 467 449 368
346 335 301 259 246 201 174 101
89 86 23 13 12
BtgI C'CryG_G
Cuts at: 107 1247 3326 5660 107
Size: 1140 2079 2334 3677
Fragments arranged by size:
3677 2334 2079 1140
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 7180 7361 7524 7180
Size: 181 163 8886
Fragments arranged by size:
8886 181 163
BtsI GCAGTG_nn'
Cuts at: 2269 6473 2269
Size: 4204 5026
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2247 2282 2973 3008 5591 5626 2247
Size: 35 691 35 2583 35 5851
Fragments arranged by size:
5851 2583 691 35 35 35
DraI TTT'AAA
Cuts at: 2597 3099 4604 5255 6229 2597
Size: 502 1505 651 974 5598
Fragments arranged by size:
5598 1505 974 651 502
DraIII CAC_nnn'GTG
Cuts at: 6037 6037
Size: 9230
DrdI GACnn_nn'nnGTC
Cuts at: 6936 7794 6936
Size: 858 8372
EaeI y'GGCC_r
Cuts at: 3250 5671 6815 6989 7380 7407 8752 3250
Size: 2421 1144 174 391 27 1345 3728
Fragments arranged by size:
3728 2421 1345 1144 391 174 27
EagI C'GGCC_G
Cuts at: 6815 6815
Size: 9230
EarI CTCTTCn'nnn_
Cuts at: 2036 2072 2196 2678 2923 3186 4502 4755 5367
Size: 36 124 482 245 263 1316 253 612
Cuts at: 5367 7253 7463 2036
Size: 1886 210 3803
Fragments arranged by size:
3803 1886 1316 612 482 263 253 245
210 124 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 7438 7748 7894 9040 7438
Size: 310 146 1146 7628
Fragments arranged by size:
7628 1146 310 146
EcoICRI GAG'CTC
Cuts at: 6242 6242
Size: 9230
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 262 752 761 1492 2441 3962 3966 4180
Size: 57 490 9 731 949 1521 4 214
Cuts at: 4180 4342 4882 5084 5506 5618 7055 7487 8234
Size: 162 540 202 422 112 1437 432 747
Cuts at: 8234 8502 205
Size: 268 933
Fragments arranged by size:
1521 1437 949 933 747 731 540 490
432 422 268 214 202 162 112 57
9 4
EcoNI CCTnn'n_nnAGG
Cuts at: 272 3814 272
Size: 3542 5688
EcoO109I rG'GnC_Cy
Cuts at: 2007 3819 4745 5563 5716 6369 2007
Size: 1812 926 818 153 653 4868
Fragments arranged by size:
4868 1812 926 818 653 153
EcoRI G'AATT_C
Cuts at: 2482 3613 6234 2482
Size: 1131 2621 5478
Fragments arranged by size:
5478 2621 1131
EcoRV GAT'ATC
Cuts at: 3854 3854
Size: 9230
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 782 2168 2854 2938 5193 7249 782
Size: 1386 686 84 2255 2056 2763
Fragments arranged by size:
2763 2255 2056 1386 686 84
FspI TGC'GCA
Cuts at: 7011 7011
Size: 9230
HaeII r_GCGC'y
Cuts at: 3999 5796 6664 6912 7934 8913 3999
Size: 1797 868 248 1022 979 4316
Fragments arranged by size:
4316 1797 1022 979 868 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 838 870 1111 1143 1309 1341 2206 2238 2894
Size: 32 241 32 166 32 865 32 656
Cuts at: 2894 2926 3239 3271 3394 3426 3568 3600 3843
Size: 32 313 32 123 32 142 32 243
Cuts at: 3843 3875 4767 4799 5703 5735 5917 5949 7072
Size: 32 892 32 904 32 182 32 1123
Cuts at: 7072 7102 7104 7134 838
Size: 30 2 30 2934
Fragments arranged by size:
2934 1123 904 892 865 656 313 243
241 182 166 142 123 32 32 32
32 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3402 3430 3650 6269 3402
Size: 28 220 2619 6363
Fragments arranged by size:
6363 2619 220 28
HindIII A'AGCT_T
Cuts at: 920 2518 2774 920
Size: 1598 256 7376
Fragments arranged by size:
7376 1598 256
KasI G'GCGC_C
Cuts at: 5792 6660 6908 5792
Size: 868 248 8114
Fragments arranged by size:
8114 868 248
KpnI G_GTAC'C
Cuts at: 739 2959 6183 6250 739
Size: 2220 3224 67 3719
Fragments arranged by size:
3719 3224 2220 67
MluI A'CGCG_T
Cuts at: 8663 8663
Size: 9230
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 203 1319 4396 5388 7901 8085 203
Size: 1116 3077 992 2513 184 1348
Fragments arranged by size:
3077 2513 1348 1116 992 184
MscI TGG'CCA
Cuts at: 5673 6991 5673
Size: 1318 7912
MslI CAynn'nnrTG
Cuts at: 155 1100 2923 3214 3275 7219 7346 155
Size: 945 1823 291 61 3944 127 2039
Fragments arranged by size:
3944 2039 1823 945 291 127 61
MspA1I CmG'CkG
Cuts at: 6341 6655 7015 8028 8273 6341
Size: 314 360 1013 245 7298
Fragments arranged by size:
7298 1013 360 314 245
NaeI GCC'GGC
Cuts at: 7411 7411
Size: 9230
NarI GG'CG_CC
Cuts at: 5793 6661 6909 5793
Size: 868 248 8114
Fragments arranged by size:
8114 868 248
NcoI C'CATG_G
Cuts at: 107 1247 3326 5660 107
Size: 1140 2079 2334 3677
Fragments arranged by size:
3677 2334 2079 1140
NgoMIV G'CCGG_C
Cuts at: 7409 7409
Size: 9230
NheI G'CTAG_C
Cuts at: 2664 2664
Size: 9230
NsiI A_TGCA'T
Cuts at: 3582 3582
Size: 9230
NspI r_CATG'y
Cuts at: 252 1963 3753 5148 5420 5592 6283 6783 7690
Size: 1711 1790 1395 272 172 691 500 907
Cuts at: 7690 252
Size: 1792
Fragments arranged by size:
1792 1790 1711 1395 907 691 500 272
172
PciI A'CATG_T
Cuts at: 1959 5416 7686 1959
Size: 3457 2270 3503
Fragments arranged by size:
3503 3457 2270
PflMI CCAn_nnn'nTGG
Cuts at: 3793 5641 3793
Size: 1848 7382
PfoI T'CCnGG_A
Cuts at: 834 834
Size: 9230
PmeI GTTT'AAAC
Cuts at: 6229 6229
Size: 9230
PmlI CAC'GTG
Cuts at: 2034 2034
Size: 9230
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8369 8401 8369
Size: 32 9198
PpuMI rG'GwC_Cy
Cuts at: 3819 4745 5716 3819
Size: 926 971 7333
Fragments arranged by size:
7333 971 926
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 416 448 416
Size: 32 9198
PstI C_TGCA'G
Cuts at: 2880 4764 5220 6277 6962 2880
Size: 1884 456 1057 685 5148
Fragments arranged by size:
5148 1884 1057 685 456
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9230
PvuII CAG'CTG
Cuts at: 6655 7015 6655
Size: 360 8870
RsrII CG'GwC_CG
Cuts at: 7425 7425
Size: 9230
SacI G_AGCT'C
Cuts at: 6244 6244
Size: 9230
SalI G'TCGA_C
Cuts at: 6267 6267
Size: 9230
SapI GCTCTTCn'nnn_
Cuts at: 2036 7253 7463 2036
Size: 5217 210 3803
Fragments arranged by size:
5217 3803 210
SbfI CC_TGCA'GG
Cuts at: 6277 6277
Size: 9230
ScaI AGT'ACT
Cuts at: 1389 1476 3107 4188 8747 1389
Size: 87 1631 1081 4559 1872
Fragments arranged by size:
4559 1872 1631 1081 87
SexAI A'CCwGG_T
Cuts at: 2784 5762 2784
Size: 2978 6252
SfcI C'TryA_G
Cuts at: 33 1466 2474 2634 2876 3491 4268 4706 4760
Size: 1433 1008 160 242 615 777 438 54
Cuts at: 4760 4811 4998 5216 6273 6958 7951 8142 33
Size: 51 187 218 1057 685 993 191 1121
Fragments arranged by size:
1433 1121 1057 1008 993 777 685 615
438 242 218 191 187 160 54 51
SfoI GGC'GCC
Cuts at: 5794 6662 6910 5794
Size: 868 248 8114
Fragments arranged by size:
8114 868 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9230
SmaI CCC'GGG
Cuts at: 6252 6252
Size: 9230
SmlI C'TyrA_G
Cuts at: 447 1114 2363 5300 5530 6385 7300 7792 8054
Size: 667 1249 2937 230 855 915 492 262
Cuts at: 8054 447
Size: 1623
Fragments arranged by size:
2937 1623 1249 915 855 667 492 262
230
SpeI A'CTAG_T
Cuts at: 67 1384 3424 4189 67
Size: 1317 2040 765 5108
Fragments arranged by size:
5108 2040 1317 765
SphI G_CATG'C
Cuts at: 6283 6783 6283
Size: 500 8730
SspI AAT'ATT
Cuts at: 3600 4695 5349 3600
Size: 1095 654 7481
Fragments arranged by size:
7481 1095 654
StyI C'CwwG_G
Cuts at: 107 1247 3326 3905 5062 5660 5806 6364 6718
Size: 1140 2079 579 1157 598 146 558 354
Cuts at: 6718 107
Size: 2619
Fragments arranged by size:
2619 2079 1157 1140 598 579 558 354
146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2069 4045 5378 5511 6813 6946 7405 7614 2069
Size: 1976 1333 133 1302 133 459 209 3685
Fragments arranged by size:
3685 1976 1333 1302 459 209 133 133
TatI w'GTAC_w
Cuts at: 1156 1387 1474 1830 1863 2844 3105 4186 5482
Size: 231 87 356 33 981 261 1081 1296
Cuts at: 5482 8745 1156
Size: 3263 1641
Fragments arranged by size:
3263 1641 1296 1081 981 356 261 231
87 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1605 1637 5194 5226 8747 8779 1605
Size: 32 3557 32 3521 32 2056
Fragments arranged by size:
3557 3521 2056 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5081 7027 5081
Size: 1946 7284
XbaI T'CTAG_A
Cuts at: 6261 6261
Size: 9230
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3357 6725 3357
Size: 3368 5862
XmaI C'CCGG_G
Cuts at: 6250 6250
Size: 9230
XmnI GAAnn'nnTTC
Cuts at: 4367 4367
Size: 9230
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NsiI NspI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AleI AlfI AloI ApaI AscI BsiWI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI FseI FspAI
HpaI MfeI NdeI NotI NruI PacI PasI PshAI
PsiI PspOMI PspXI SacII SanDI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI