Restriction map for
pF1KA0246
(Circular) (Six-base) MAPSORT of: pF1KA0246.seq Check: 5700 from: 1 to: 10823
pF1KA0246 10823 bp
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 174 749 1018 1190 2612 2914 3004 4432 4564
Size: 575 269 172 1422 302 90 1428 132
Cuts at: 4564 4783 4855 5770 174
Size: 219 72 915 5227
Fragments arranged by size:
5227 1428 1422 915 575 302 269 219
172 132 90 72
AatII G_ACGT'C
Cuts at: 5860 5860
Size: 10823
AccI GT'mk_AC
Cuts at: 1371 1982 2183 3995 7861 1371
Size: 611 201 1812 3866 4333
Fragments arranged by size:
4333 3866 1812 611 201
Acc65I G'GTAC_C
Cuts at: 1832 7839 1832
Size: 6007 4816
AclI AA'CG_TT
Cuts at: 10663 10663
Size: 10823
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 150 1647 2520 3045 3513 4884 5112 5310 5359
Size: 1497 873 525 468 1371 228 198 49
Cuts at: 5359 7541 8648 9080 9827 10095 150
Size: 2182 1107 432 747 268 878
Fragments arranged by size:
2182 1497 1371 1107 878 873 747 525
468 432 268 228 198 49
AflII C'TTAA_G
Cuts at: 3130 3130
Size: 10823
AflIII A'CryG_T
Cuts at: 268 2134 2521 4036 4804 8175 9279 10256 268
Size: 1866 387 1515 768 3371 1104 977 835
Fragments arranged by size:
3371 1866 1515 1104 977 835 768 387
AgeI A'CCGG_T
Cuts at: 569 1088 9211 569
Size: 519 8123 2181
Fragments arranged by size:
8123 2181 519
AleI CACnn'nnGTG
Cuts at: 1431 4249 1431
Size: 2818 8005
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 622 781 2047 6405 6721 622
Size: 159 1266 4358 316 4724
Fragments arranged by size:
4724 4358 1266 316 159
AlwNI CAG_nnn'CTG
Cuts at: 189 711 1914 2237 3510 5697 5771 5894 5942
Size: 522 1203 323 1273 2187 74 123 48
Cuts at: 5942 5984 6093 6627 6852 7372 7385 8128 9695
Size: 42 109 534 225 520 13 743 1567
Cuts at: 9695 189
Size: 1317
Fragments arranged by size:
2187 1567 1317 1273 1203 743 534 522
520 323 225 123 109 74 48 42
13
ApaI G_GGCC'C
Cuts at: 119 1294 1727 2579 2887 3270 3748 4238 5551
Size: 1175 433 852 308 383 478 490 1313
Cuts at: 5551 6155 6504 7392 119
Size: 604 349 888 3550
Fragments arranged by size:
3550 1313 1175 888 852 604 490 478
433 383 349 308
ApaLI G'TGCA_C
Cuts at: 607 6299 6696 9593 607
Size: 5692 397 2897 1837
Fragments arranged by size:
5692 2897 1837 397
ApoI r'AATT_y
Cuts at: 7827 10176 10432 7827
Size: 2349 256 8218
Fragments arranged by size:
8218 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 10823
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10823
AvaI C'yCGr_G
Cuts at: 119 1957 2887 3214 3470 4019 4856 5239 5277
Size: 1838 930 327 256 549 837 383 38
Cuts at: 5277 6304 7610 7843 119
Size: 1027 1306 233 3099
Fragments arranged by size:
3099 1838 1306 1027 930 837 549 383
327 256 233 38
AvrII C'CTAG_G
Cuts at: 4369 4369
Size: 10823
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 215 248 215
Size: 33 10790
BamHI G'GATC_C
Cuts at: 1617 1889 2354 7802 7848 1617
Size: 272 465 5448 46 4592
Fragments arranged by size:
5448 4592 465 272 46
BanI G'GyrC_C
Cuts at: 431 761 1308 1832 2761 3180 3283 3519 3567
Size: 330 547 524 929 419 103 236 48
Cuts at: 3567 3671 4056 4533 5114 6142 6964 7488 7515
Size: 104 385 477 581 1028 822 524 27
Cuts at: 7515 7839 8253 8501 8536 431
Size: 324 414 248 35 2718
Fragments arranged by size:
2718 1028 929 822 581 547 524 524
477 419 414 385 330 324 248 236
104 103 48 35 27
BanII G_rGCy'C
Cuts at: 119 296 346 804 859 1294 1727 2101 2207
Size: 177 50 458 55 435 433 374 106
Cuts at: 2207 2579 2887 3214 3270 3748 3877 4238 4727
Size: 372 308 327 56 478 129 361 489
Cuts at: 4727 4874 4931 5427 5551 5615 6155 6214 6504
Size: 147 57 496 124 64 540 59 290
Cuts at: 6504 7196 7392 7596 7837 8867 119
Size: 692 196 204 241 1030 2075
Fragments arranged by size:
2075 1030 692 540 496 489 478 458
435 433 374 372 361 327 308 290
241 204 196 177 147 129 124 106
64 59 57 56 55 50
BbeI G_GCGC'C
Cuts at: 7519 8257 8505 7519
Size: 738 248 9837
Fragments arranged by size:
9837 738 248
BbsI GAAGACnn'nnnn_
Cuts at: 1557 2459 3756 1557
Size: 902 1297 8624
Fragments arranged by size:
8624 1297 902
BbvCI CC'TCA_GC
Cuts at: 1234 3448 3902 6785 6830 7345 1234
Size: 2214 454 2883 45 515 4712
Fragments arranged by size:
4712 2883 2214 515 454 45
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1075 1109 2028 2062 2888 2922 2931 2965 4369
Size: 34 919 34 826 34 9 34 1404
Cuts at: 4369 4403 1075
Size: 34 7495
Fragments arranged by size:
7495 1404 919 826 34 34 34 34
34 9
BciVI GTATCCnnnnn_n'
Cuts at: 658 4435 8721 9482 10823 658
Size: 3777 4286 761 1341 658
Fragments arranged by size:
4286 3777 1341 761 658
BclI T'GATC_A
Cuts at: 684 8343 684
Size: 7659 3164
BfrBI ATG'CAT
Cuts at: 1348 5565 1348
Size: 4217 6606
BglI GCCn_nnn'nGGC
Cuts at: 161 449 770 1019 2516 2583 2786 2966 3041
Size: 288 321 249 1497 67 203 180 75
Cuts at: 3041 3489 4187 4499 4565 6026 6209 6323 6599
Size: 448 698 312 66 1461 183 114 276
Cuts at: 6599 6875 7040 7396 7648 161
Size: 276 165 356 252 3336
Fragments arranged by size:
3336 1497 1461 698 448 356 321 312
288 276 276 252 249 203 183 180
165 114 75 67 66
BglII A'GATC_T
Cuts at: 4971 5321 8338 10375 4971
Size: 350 3017 2037 5419
Fragments arranged by size:
5419 3017 2037 350
BlpI GC'TnA_GC
Cuts at: 540 3208 7935 540
Size: 2668 4727 3428
Fragments arranged by size:
4727 3428 2668
Bme1580I G_kGCm'C
Cuts at: 119 611 1294 1727 2521 2529 2579 2764 2887
Size: 492 683 433 794 8 50 185 123
Cuts at: 2887 3037 3185 3270 3288 3485 3572 3611 3748
Size: 150 148 85 18 197 87 39 137
Cuts at: 3748 4238 4266 4369 4857 5117 5551 6155 6303
Size: 490 28 103 488 260 434 604 148
Cuts at: 6303 6504 6700 6969 7392 7611 8448 8541 9597
Size: 201 196 269 423 219 837 93 1056
Cuts at: 9597 119
Size: 1345
Fragments arranged by size:
1345 1056 837 794 683 604 492 490
488 434 433 423 269 260 219 201
197 196 185 150 148 148 137 123
103 93 87 85 50 39 28 18
8
BmgBI CAC'GTC
Cuts at: 269 1986 2841 269
Size: 1717 855 8251
Fragments arranged by size:
8251 1717 855
BmrI ACTGGGnnnn_n'
Cuts at: 429 726 837 5262 6254 8035 8116 8451 10595
Size: 297 111 4425 992 1781 81 335 2144
Cuts at: 10595 429
Size: 657
Fragments arranged by size:
4425 2144 1781 992 657 335 297 111
81
BmtI G_CTAG'C
Cuts at: 5482 5482
Size: 10823
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3714 9190 3714
Size: 5476 5347
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1317 1423 1705 2811 3112 3262 3646 3844 4888
Size: 106 282 1106 301 150 384 198 1044
Cuts at: 4888 5104 5719 5800 6289 6510 6649 6727 7586
Size: 216 615 81 489 221 139 78 859
Cuts at: 7586 7795 1317
Size: 209 4345
Fragments arranged by size:
4345 1106 1044 859 615 489 384 301
282 221 216 209 198 150 139 106
81 78
Bpu10I CC'TnA_GC
Cuts at: 1234 3448 3902 4128 6785 6830 7345 1234
Size: 2214 454 226 2657 45 515 4712
Fragments arranged by size:
4712 2657 2214 515 454 226 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3861 4915 6579 7999 8878 9370 9668 3861
Size: 1054 1664 1420 879 492 298 5016
Fragments arranged by size:
5016 1664 1420 1054 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 451 1810 2479 4677 5731 7168 7716 10527 451
Size: 1359 669 2198 1054 1437 548 2811 747
Fragments arranged by size:
2811 2198 1437 1359 1054 747 669 548
BsaAI yAC'GTr
Cuts at: 634 1572 2135 2522 3032 5046 6606 7067 7199
Size: 938 563 387 510 2014 1560 461 132
Cuts at: 7199 8176 634
Size: 977 3281
Fragments arranged by size:
3281 2014 1560 977 938 563 510 461
387 132
BsaBI GATnn'nnATC
Cuts at: 6 8337 10374 6
Size: 8331 2037 455
Fragments arranged by size:
8331 2037 455
BsaHI Gr'CG_yC
Cuts at: 1562 5411 5857 7516 8254 8502 9204 10700 1562
Size: 3849 446 1659 738 248 702 1496 1685
Fragments arranged by size:
3849 1685 1659 1496 738 702 446 248
BsaWI w'CCGG_w
Cuts at: 569 1088 4246 4712 5433 8012 8533 9211 9485
Size: 519 3158 466 721 2579 521 678 274
Cuts at: 9485 9632 10043 569
Size: 147 411 1349
Fragments arranged by size:
3158 2579 1349 721 678 521 519 466
411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 125 155 1169 1199 2686 2716 3718 3748 5077
Size: 30 1014 30 1487 30 1002 30 1329
Cuts at: 5077 5107 5847 5877 125
Size: 30 740 30 5071
Fragments arranged by size:
5071 1487 1329 1014 1002 740 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 118 1311 1320 1948 2089 3310 3313 5295 6646
Size: 1193 9 628 141 1221 3 1982 1351
Cuts at: 6646 7792 118
Size: 1146 3149
Fragments arranged by size:
3149 1982 1351 1221 1193 1146 628 141
9 3
BseYI C'CCAG_C
Cuts at: 336 751 958 1358 1514 1950 2591 2913 3312
Size: 415 207 400 156 436 641 322 399
Cuts at: 3312 3353 4221 4664 5905 5977 6031 6112 6845
Size: 41 868 443 1241 72 54 81 733
Cuts at: 6845 6852 6989 7154 8248 9006 9583 10079 336
Size: 7 137 165 1094 758 577 496 1080
Fragments arranged by size:
1241 1094 1080 868 758 733 641 577
496 443 436 415 400 399 322 207
165 156 137 81 72 54 41 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 222 415 618 990 1158 1216 2277 2667 2757
Size: 193 203 372 168 58 1061 390 90
Cuts at: 2757 2961 3012 3409 3915 3933 4227 4420 5194
Size: 204 51 397 506 18 294 193 774
Cuts at: 5194 5233 5665 5854 6159 6341 6360 6498 6691
Size: 39 432 189 305 182 19 138 193
Cuts at: 6691 222
Size: 4354
Fragments arranged by size:
4354 1061 774 506 432 397 390 372
305 294 204 203 193 193 193 189
182 168 138 90 58 51 39 19
18
BsiEI CG_ry'CG
Cuts at: 104 1089 1779 3162 4412 8365 8411 9619 104
Size: 985 690 1383 1250 3953 46 1208 1308
Fragments arranged by size:
3953 1383 1308 1250 1208 985 690 46
BsiHKAI G_wGCw'C
Cuts at: 296 611 739 850 4129 4727 4874 4931 5427
Size: 315 128 111 3279 598 147 57 496
Cuts at: 5427 5615 5721 6138 6303 6422 6548 6565 6700
Size: 188 106 417 165 119 126 17 135
Cuts at: 6700 7196 7572 7596 7837 8615 8805 9597 296
Size: 496 376 24 241 778 190 792 1522
Fragments arranged by size:
3279 1522 792 778 598 496 496 417
376 315 241 190 188 165 147 135
128 126 119 111 106 57 24 17
BsmI GAATG_Cn'
Cuts at: 445 799 1350 2739 3399 445
Size: 354 551 1389 660 7869
Fragments arranged by size:
7869 1389 660 551 354
BsmBI CGTCTCn'nnnn_
Cuts at: 1323 1323
Size: 10823
Bsp1286I G_dGCh'C
Cuts at: 119 296 346 611 739 804 850 859 1294
Size: 177 50 265 128 65 46 9 435
Cuts at: 1294 1727 2101 2207 2521 2529 2579 2764 2887
Size: 433 374 106 314 8 50 185 123
Cuts at: 2887 3037 3185 3214 3270 3288 3485 3572 3611
Size: 150 148 29 56 18 197 87 39
Cuts at: 3611 3748 3877 4129 4238 4266 4369 4727 4857
Size: 137 129 252 109 28 103 358 130
Cuts at: 4857 4874 4931 5117 5427 5551 5615 5721 6138
Size: 17 57 186 310 124 64 106 417
Cuts at: 6138 6155 6214 6303 6422 6504 6548 6565 6700
Size: 17 59 89 119 82 44 17 135
Cuts at: 6700 6969 7196 7392 7572 7596 7611 7837 8448
Size: 269 227 196 180 24 15 226 611
Cuts at: 8448 8541 8615 8805 8867 9597 119
Size: 93 74 190 62 730 1345
Fragments arranged by size:
1345 730 611 435 433 417 374 358
314 310 269 265 252 227 226 197
196 190 186 185 180 177 150 148
137 135 130 129 128 124 123 119
109 106 106 103 93 89 87 82
74 65 64 62 59 57 56 50
50 46 44 39 29 28 24 18
17 17 17 15 9 8
BspEI T'CCGG_A
Cuts at: 4712 5433 10043 4712
Size: 721 4610 5492
Fragments arranged by size:
5492 4610 721
BspHI T'CATG_A
Cuts at: 9999 9999
Size: 10823
BspMI ACCTGCnnnn'nnnn_
Cuts at: 174 490 749 1018 1190 2612 2914 3004 4432
Size: 316 259 269 172 1422 302 90 1428
Cuts at: 4432 4564 4659 4726 4783 4819 4855 5087 5543
Size: 132 95 67 57 36 36 232 456
Cuts at: 5543 5770 6469 6713 7873 8087 8389 8770 174
Size: 227 699 244 1160 214 302 381 2227
Fragments arranged by size:
2227 1428 1422 1160 699 456 381 316
302 302 269 259 244 232 227 214
172 132 95 90 67 57 36 36
BsrBI CCG'CTC
Cuts at: 50 362 3620 3839 9115 9169 10653 10818 50
Size: 312 3258 219 5276 54 1484 165 55
Fragments arranged by size:
5276 3258 1484 312 219 165 55 54
BsrDI GCAATG_nn'
Cuts at: 474 1755 2370 2449 2976 4458 5517 6942 7077
Size: 1281 615 79 527 1482 1059 1425 135
Cuts at: 7077 8735 474
Size: 1658 2562
Fragments arranged by size:
2562 1658 1482 1425 1281 1059 615 527
135 79
BsrFI r'CCGG_y
Cuts at: 569 1013 1088 2543 3139 5347 6565 8821 9002
Size: 444 75 1455 596 2208 1218 2256 181
Cuts at: 9002 9211 569
Size: 209 2181
Fragments arranged by size:
2256 2208 2181 1455 1218 596 444 209
181 75
BsrGI T'GTAC_A
Cuts at: 6373 6373
Size: 10823
BssHII G'CGCG_C
Cuts at: 5167 5167
Size: 10823
BssSI C'ACGA_G
Cuts at: 243 1189 4183 9094 9452 243
Size: 946 2994 4911 358 1614
Fragments arranged by size:
4911 2994 1614 946 358
BstAPI GCAn_nnn'nTGC
Cuts at: 245 393 794 1354 2405 2699 3498 5121 5942
Size: 148 401 560 1051 294 799 1623 821
Cuts at: 5942 6008 6512 8200 245
Size: 66 504 1688 2868
Fragments arranged by size:
2868 1688 1623 1051 821 799 560 504
401 294 148 66
BstBI TT'CG_AA
Cuts at: 5556 9184 5556
Size: 3628 7195
BstEII G'GTnAC_C
Cuts at: 1107 1107
Size: 10823
BstXI CCAn_nnnn'nTGG
Cuts at: 904 2365 2586 4495 5050 904
Size: 1461 221 1909 555 6677
Fragments arranged by size:
6677 1909 1461 555 221
BstYI r'GATC_y
Cuts at: 1 1617 1640 1889 2354 4049 4971 5321 7802
Size: 1616 23 249 465 1695 922 350 2481
Cuts at: 7802 7848 8315 8338 8673 8919 9931 10017 10029
Size: 46 467 23 335 246 1012 86 12
Cuts at: 10029 10375 1
Size: 346 449
Fragments arranged by size:
2481 1695 1616 1012 922 467 465 449
350 346 335 249 246 86 46 23
23 12
Bsu36I CC'TnA_GG
Cuts at: 276 3788 4876 5795 5822 276
Size: 3512 1088 919 27 5277
Fragments arranged by size:
5277 3512 1088 919 27
BtgI C'CryG_G
Cuts at: 107 474 534 624 897 1244 1848 1898 2469
Size: 367 60 90 273 347 604 50 571
Cuts at: 2469 2561 2715 2982 3558 3701 4134 4194 4266
Size: 92 154 267 576 143 433 60 72
Cuts at: 4266 4488 4494 4620 4829 5099 6051 6528 7622
Size: 222 6 126 209 270 952 477 1094
Cuts at: 7622 7681 107
Size: 59 3249
Fragments arranged by size:
3249 1094 952 604 576 571 477 433
367 347 273 270 267 222 209 154
143 126 92 90 72 60 60 59
50 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 917 1368 2385 4952 5005 6626 8773 8954 9117
Size: 451 1017 2567 53 1621 2147 181 163
Cuts at: 9117 917
Size: 2623
Fragments arranged by size:
2623 2567 2147 1621 1017 451 181 163
53
BtsI GCAGTG_nn'
Cuts at: 795 832 879 2064 2305 2352 2502 2595 2769
Size: 37 47 1185 241 47 150 93 174
Cuts at: 2769 3011 3587 4147 4176 5119 6009 6087 6094
Size: 242 576 560 29 943 890 78 7
Cuts at: 6094 6420 6628 6896 7100 7514 7570 7768 8066
Size: 326 208 268 204 414 56 198 298
Cuts at: 8066 795
Size: 3552
Fragments arranged by size:
3552 1185 943 890 576 560 414 326
298 268 242 241 208 204 198 174
150 93 78 56 47 47 37 29
7
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 930 965 3354 3389 3759 3794 5935 5970 930
Size: 35 2389 35 370 35 2141 35 5783
Fragments arranged by size:
5783 2389 2141 370 35 35 35 35
DraI TTT'AAA
Cuts at: 7822 7822
Size: 10823
DraIII CAC_nnn'GTG
Cuts at: 245 615 734 1194 2300 3678 4091 4830 6256
Size: 370 119 460 1106 1378 413 739 1426
Cuts at: 6256 6298 6909 245
Size: 42 611 4159
Fragments arranged by size:
4159 1426 1378 1106 739 611 460 413
370 119 42
DrdI GACnn_nn'nnGTC
Cuts at: 3166 6623 8529 9387 3166
Size: 3457 1906 858 4602
Fragments arranged by size:
4602 3457 1906 858
EaeI y'GGCC_r
Cuts at: 251 471 817 1015 1247 1445 1776 1851 1991
Size: 220 346 198 232 198 331 75 140
Cuts at: 1991 2541 2802 2990 3329 3780 3834 4409 4491
Size: 550 261 188 339 451 54 575 82
Cuts at: 4491 4794 5345 6186 6363 6522 6884 7472 7586
Size: 303 551 841 177 159 362 588 114
Cuts at: 7586 8408 8582 8973 9000 10345 251
Size: 822 174 391 27 1345 729
Fragments arranged by size:
1345 841 822 729 588 575 551 550
451 391 362 346 339 331 303 261
232 220 198 198 188 177 174 159
140 114 82 75 54 27
EagI C'GGCC_G
Cuts at: 1776 4409 8408 1776
Size: 2633 3999 4191
Fragments arranged by size:
4191 3999 2633
EarI CTCTTCn'nnn_
Cuts at: 1798 3295 3541 3979 7216 8846 9056 1798
Size: 1497 246 438 3237 1630 210 3565
Fragments arranged by size:
3565 3237 1630 1497 438 246 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4897 6940 7666 9031 9341 9487 10633 4897
Size: 2043 726 1365 310 146 1146 5087
Fragments arranged by size:
5087 2043 1365 1146 726 310 146
EcoICRI GAG'CTC
Cuts at: 294 4725 4872 4929 5425 5613 7194 7594 7835
Size: 4431 147 57 496 188 1581 400 241
Cuts at: 7835 294
Size: 3282
Fragments arranged by size:
4431 3282 1581 496 400 241 188 147
57
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 150 1317 1423 1647 1705 2520 2811 3045 3112
Size: 1167 106 224 58 815 291 234 67
Cuts at: 3112 3262 3513 3646 3844 4884 4888 5104 5112
Size: 150 251 133 198 1040 4 216 8
Cuts at: 5112 5310 5359 5719 5800 6289 6510 6649 6727
Size: 198 49 360 81 489 221 139 78
Cuts at: 6727 7541 7586 7795 8648 9080 9827 10095 150
Size: 814 45 209 853 432 747 268 878
Fragments arranged by size:
1167 1040 878 853 815 814 747 489
432 360 291 268 251 234 224 221
216 209 198 198 150 139 133 106
81 78 67 58 49 45 8 4
EcoNI CCTnn'n_nnAGG
Cuts at: 3292 3451 3688 3715 4990 5338 6391 3292
Size: 159 237 27 1275 348 1053 7724
Fragments arranged by size:
7724 1275 1053 348 237 159 27
EcoO109I rG'GnC_Cy
Cuts at: 115 116 123 479 509 927 1291 1415 1596
Size: 1 7 356 30 418 364 124 181
Cuts at: 1596 1607 1724 1911 1954 1965 2369 2753 2884
Size: 11 117 187 43 11 404 384 131
Cuts at: 2884 3266 3693 3745 3814 3828 3861 3906 4329
Size: 382 427 52 69 14 33 45 423
Cuts at: 4329 5076 5384 5570 5921 6152 6201 7369 7389
Size: 747 308 186 351 231 49 1168 20
Cuts at: 7389 7962 115
Size: 573 2976
Fragments arranged by size:
2976 1168 747 573 427 423 418 404
384 382 364 356 351 308 231 187
186 181 131 124 117 69 52 49
45 43 33 30 20 14 11 11
7 1
EcoRI G'AATT_C
Cuts at: 7827 7827
Size: 10823
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2934 7227 8842 2934
Size: 4293 1615 4915
Fragments arranged by size:
4915 4293 1615
FseI GG_CCGG'CC
Cuts at: 5351 5351
Size: 10823
FspI TGC'GCA
Cuts at: 5018 8604 5018
Size: 3586 7237
FspAI rTGC'GCAy
Cuts at: 5018 5018
Size: 10823
HaeII r_GCGC'y
Cuts at: 455 5248 5838 6585 7519 8257 8505 9527 10506
Size: 4793 590 747 934 738 248 1022 979
Cuts at: 10506 455
Size: 772
Fragments arranged by size:
4793 1022 979 934 772 747 738 590
248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 574 606 900 932 1169 1201 1364 1396 1628
Size: 32 294 32 237 32 163 32 232
Cuts at: 1628 1660 2662 2694 4723 4755 5847 5879 7660
Size: 32 1002 32 2029 32 1092 32 1781
Cuts at: 7660 7692 8665 8695 8697 8727 574
Size: 32 973 30 2 30 2670
Fragments arranged by size:
2670 2029 1781 1092 1002 973 294 237
232 163 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 7862 7862
Size: 10823
HindIII A'AGCT_T
Cuts at: 6014 6014
Size: 10823
KasI G'GCGC_C
Cuts at: 7515 8253 8501 7515
Size: 738 248 9837
Fragments arranged by size:
9837 738 248
KpnI G_GTAC'C
Cuts at: 1836 7843 1836
Size: 6007 4816
MfeI C'AATT_G
Cuts at: 2340 5952 2340
Size: 3612 7211
MluI A'CGCG_T
Cuts at: 10256 10256
Size: 10823
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1867 2342 6617 7307 9494 9678 1867
Size: 475 4275 690 2187 184 3012
Fragments arranged by size:
4275 3012 2187 690 475 184
MscI TGG'CCA
Cuts at: 473 819 1249 2992 3331 3782 4796 6524 6886
Size: 346 430 1743 339 451 1014 1728 362
Cuts at: 6886 7474 8584 473
Size: 588 1110 2712
Fragments arranged by size:
2712 1743 1728 1110 1014 588 451 430
362 346 339
MslI CAynn'nnrTG
Cuts at: 902 1431 2606 2861 3604 4249 4493 4957 6686
Size: 529 1175 255 743 645 244 464 1729
Cuts at: 6686 8812 8939 902
Size: 2126 127 2786
Fragments arranged by size:
2786 2126 1729 1175 743 645 529 464
255 244 127
MspA1I CmG'CkG
Cuts at: 336 536 602 755 1114 1130 1233 1288 1801
Size: 200 66 153 359 16 103 55 513
Cuts at: 1801 1857 1971 2615 3021 3065 3207 3510 3947
Size: 56 114 644 406 44 142 303 437
Cuts at: 3947 3966 4166 4337 4545 4607 4682 4700 5028
Size: 19 200 171 208 62 75 18 328
Cuts at: 5028 5330 5649 5694 5868 5981 6173 6191 6332
Size: 302 319 45 174 113 192 18 141
Cuts at: 6332 6530 6656 6755 6852 7158 7564 7934 8248
Size: 198 126 99 97 306 406 370 314
Cuts at: 8248 8608 9621 9866 336
Size: 360 1013 245 1293
Fragments arranged by size:
1293 1013 644 513 437 406 406 370
360 359 328 319 314 306 303 302
245 208 200 200 198 192 174 171
153 142 141 126 114 113 103 99
97 75 66 62 56 55 45 44
19 18 18 16
NaeI GCC'GGC
Cuts at: 1015 3141 5349 9004 1015
Size: 2126 2208 3655 2834
Fragments arranged by size:
3655 2834 2208 2126
NarI GG'CG_CC
Cuts at: 7516 8254 8502 7516
Size: 738 248 9837
Fragments arranged by size:
9837 738 248
NcoI C'CATG_G
Cuts at: 107 624 897 1898 2469 2561 3558 3701 4134
Size: 517 273 1001 571 92 997 143 433
Cuts at: 4134 4194 4266 4494 7622 107
Size: 60 72 228 3128 3308
Fragments arranged by size:
3308 3128 1001 997 571 517 433 273
228 143 92 72 60
NdeI CA'TA_TG
Cuts at: 4767 4767
Size: 10823
NgoMIV G'CCGG_C
Cuts at: 1013 3139 5347 9002 1013
Size: 2126 2208 3655 2834
Fragments arranged by size:
3655 2834 2208 2126
NheI G'CTAG_C
Cuts at: 5478 5478
Size: 10823
NsiI A_TGCA'T
Cuts at: 1350 5567 1350
Size: 4217 6606
NspI r_CATG'y
Cuts at: 2016 2611 2780 4040 4553 4808 4891 6797 7279
Size: 595 169 1260 513 255 83 1906 482
Cuts at: 7279 7876 8376 9283 2016
Size: 597 500 907 3556
Fragments arranged by size:
3556 1906 1260 907 597 595 513 500
482 255 169 83
PasI CC'CwG_GG
Cuts at: 445 1612 2749 2757 3633 4105 5304 7402 445
Size: 1167 1137 8 876 472 1199 2098 3866
Fragments arranged by size:
3866 2098 1199 1167 1137 876 472 8
PciI A'CATG_T
Cuts at: 4036 4804 9279 4036
Size: 768 4475 5580
Fragments arranged by size:
5580 4475 768
PflMI CCAn_nnn'nTGG
Cuts at: 469 1914 2016 2497 2988 3353 3564 4830 5697
Size: 1445 102 481 491 365 211 1266 867
Cuts at: 5697 5912 5931 5969 6852 6909 7470 469
Size: 215 19 38 883 57 561 3822
Fragments arranged by size:
3822 1445 1266 883 867 561 491 481
365 215 211 102 57 38 19
PfoI T'CCnGG_A
Cuts at: 296 1333 1400 1682 1709 3083 3539 4727 4913
Size: 1037 67 282 27 1374 456 1188 186
Cuts at: 4913 6713 7172 7181 296
Size: 1800 459 9 3938
Fragments arranged by size:
3938 1800 1374 1188 1037 459 456 282
186 67 27 9
PmeI GTTT'AAAC
Cuts at: 7822 7822
Size: 10823
PmlI CAC'GTG
Cuts at: 634 1572 2135 2522 3032 5046 7067 634
Size: 938 563 387 510 2014 2021 4390
Fragments arranged by size:
4390 2021 2014 938 563 510 387
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9962 9994 9962
Size: 32 10791
PpuMI rG'GwC_Cy
Cuts at: 479 509 927 1415 1607 1911 1954 1965 2753
Size: 30 418 488 192 304 43 11 788
Cuts at: 2753 3861 5076 5570 479
Size: 1108 1215 494 5732
Fragments arranged by size:
5732 1215 1108 788 494 488 418 304
192 43 30 11
PspOMI G'GGCC_C
Cuts at: 115 1290 1723 2575 2883 3266 3744 4234 5547
Size: 1175 433 852 308 383 478 490 1313
Cuts at: 5547 6151 6500 7388 115
Size: 604 349 888 3550
Fragments arranged by size:
3550 1313 1175 888 852 604 490 478
433 383 349 308
PstI C_TGCA'G
Cuts at: 370 760 1232 1820 1976 2071 2875 3198 3239
Size: 390 472 588 156 95 804 323 41
Cuts at: 3239 3506 3554 3971 4681 5593 5767 6278 6415
Size: 267 48 417 710 912 174 511 137
Cuts at: 6415 6541 6623 7870 8555 370
Size: 126 82 1247 685 2638
Fragments arranged by size:
2638 1247 912 804 710 685 588 511
472 417 390 323 267 174 156 137
126 95 82 48 41
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10823
PvuII CAG'CTG
Cuts at: 336 602 755 1130 1233 1857 3065 3207 3510
Size: 266 153 375 103 624 1208 142 303
Cuts at: 3510 3966 4607 4682 5028 5694 5981 6332 6852
Size: 456 641 75 346 666 287 351 520
Cuts at: 6852 8248 8608 336
Size: 1396 360 2551
Fragments arranged by size:
2551 1396 1208 666 641 624 520 456
375 360 351 346 303 287 266 153
142 103 75
RsrII CG'GwC_CG
Cuts at: 2712 9018 2712
Size: 6306 4517
SacI G_AGCT'C
Cuts at: 296 4727 4874 4931 5427 5615 7196 7596 7837
Size: 4431 147 57 496 188 1581 400 241
Cuts at: 7837 296
Size: 3282
Fragments arranged by size:
4431 3282 1581 496 400 241 188 147
57
SacII CC_GC'GG
Cuts at: 537 6531 537
Size: 5994 4829
SalI G'TCGA_C
Cuts at: 7860 7860
Size: 10823
SanDI GG'GwC_CC
Cuts at: 1607 1954 1965 2753 5570 1607
Size: 347 11 788 2817 6860
Fragments arranged by size:
6860 2817 788 347 11
SapI GCTCTTCn'nnn_
Cuts at: 3979 8846 9056 3979
Size: 4867 210 5746
Fragments arranged by size:
5746 4867 210
SbfI CC_TGCA'GG
Cuts at: 7870 7870
Size: 10823
ScaI AGT'ACT
Cuts at: 10340 10340
Size: 10823
SexAI A'CCwGG_T
Cuts at: 1907 3257 1907
Size: 1350 9473
SfcI C'TryA_G
Cuts at: 33 366 756 1228 1368 1816 1972 2067 2871
Size: 333 390 472 140 448 156 95 804
Cuts at: 2871 3194 3235 3502 3550 3967 4677 5589 5763
Size: 323 41 267 48 417 710 912 174
Cuts at: 5763 6274 6411 6429 6537 6619 7866 8551 9544
Size: 511 137 18 108 82 1247 685 993
Cuts at: 9544 9735 33
Size: 191 1121
Fragments arranged by size:
1247 1121 993 912 804 710 685 511
472 448 417 390 333 323 267 191
174 156 140 137 108 95 82 48
41 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 7396 7396
Size: 10823
SfoI GGC'GCC
Cuts at: 7517 8255 8503 7517
Size: 738 248 9837
Fragments arranged by size:
9837 738 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10823
SmaI CCC'GGG
Cuts at: 121 2889 4021 7845 121
Size: 2768 1132 3824 3099
Fragments arranged by size:
3824 3099 2768 1132
SmlI C'TyrA_G
Cuts at: 3130 3876 4930 6558 7978 8893 9385 9647 3130
Size: 746 1054 1628 1420 915 492 262 4306
Fragments arranged by size:
4306 1628 1420 1054 915 746 492 262
SnaBI TAC'GTA
Cuts at: 6606 6606
Size: 10823
SpeI A'CTAG_T
Cuts at: 67 67
Size: 10823
SphI G_CATG'C
Cuts at: 2611 4891 6797 7279 7876 8376 2611
Size: 2280 1906 482 597 500 5058
Fragments arranged by size:
5058 2280 1906 597 500 482
StuI AGG'CCT
Cuts at: 403 1000 1022 1660 5446 5521 5827 5930 7343
Size: 597 22 638 3786 75 306 103 1413
Cuts at: 7343 403
Size: 3883
Fragments arranged by size:
3883 3786 1413 638 597 306 103 75
22
StyI C'CwwG_G
Cuts at: 107 624 860 897 923 945 1880 1898 2171
Size: 517 236 37 26 22 935 18 273
Cuts at: 2171 2469 2561 3241 3558 3701 4134 4194 4266
Size: 298 92 680 317 143 433 60 72
Cuts at: 4266 4369 4494 4526 5522 5712 5925 6204 7622
Size: 103 125 32 996 190 213 279 1418
Cuts at: 7622 7957 8311 107
Size: 335 354 2619
Fragments arranged by size:
2619 1418 996 935 680 517 433 354
335 317 298 279 273 236 213 190
143 125 103 92 72 60 37 32
26 22 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 745 2779 3236 3304 3375 3993 5132 5611 6952
Size: 2034 457 68 71 618 1139 479 1341
Cuts at: 6952 7039 7168 7810 8406 8539 8998 9207 745
Size: 87 129 642 596 133 459 209 2361
Fragments arranged by size:
2361 2034 1341 1139 642 618 596 479
459 457 209 133 129 87 71 68
TatI w'GTAC_w
Cuts at: 330 1499 6373 10338 330
Size: 1169 4874 3965 815
Fragments arranged by size:
4874 3965 1169 815
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 125 157 2286 2318 3718 3750 3975 4007 6108
Size: 32 2129 32 1400 32 225 32 2101
Cuts at: 6108 6140 7199 7231 10340 10372 125
Size: 32 1059 32 3109 32 576
Fragments arranged by size:
3109 2129 2101 1400 1059 576 225 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5224 8620 5224
Size: 3396 7427
XbaI T'CTAG_A
Cuts at: 7854 7854
Size: 10823
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 442 631 1576 2713 3405 3621 6019 6400 7482
Size: 189 945 1137 692 216 2398 381 1082
Cuts at: 7482 7704 8318 442
Size: 222 614 2947
Fragments arranged by size:
2947 2398 1137 1082 945 692 614 381
222 216 189
XmaI C'CCGG_G
Cuts at: 119 2887 4019 7843 119
Size: 2768 1132 3824 3099
Fragments arranged by size:
3824 3099 2768 1132
ZraI GAC'GTC
Cuts at: 5858 5858
Size: 10823
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI FspAI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AhdI AloI AscI BsiWI BstZ17I ClaI EcoRV
HpaI NotI NruI PacI PshAI PsiI PspXI PsrI
SgrAI SrfI SspI SwaI XhoI XmnI