Restriction map for
pF1KA0249
(Circular) (Six-base) MAPSORT of: pF1KA0249.seq Check: 2807 from: 1 to: 5801
pF1KA0249 5801 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 126 126
Size: 5801
AccI GT'mk_AC
Cuts at: 1253 2839 1253
Size: 1586 4215
Acc65I G'GTAC_C
Cuts at: 2817 2817
Size: 5801
AclI AA'CG_TT
Cuts at: 5641 5641
Size: 5801
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 404 458 1367 2074 2792 3626 4058 4411 5073
Size: 54 909 707 718 834 432 353 662
Cuts at: 5073 404
Size: 1132
Fragments arranged by size:
1132 909 834 718 707 662 432 353
54
AflIII A'CryG_T
Cuts at: 522 646 3153 4953 5234 522
Size: 124 2507 1800 281 1089
Fragments arranged by size:
2507 1800 1089 281 124
AhdI GACnn_n'nnGTC
Cuts at: 824 1260 824
Size: 436 5365
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2395 2395
Size: 5801
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2671 2703 2671
Size: 32 5769
AlwNI CAG_nnn'CTG
Cuts at: 1492 3106 4544 1492
Size: 1614 1438 2749
Fragments arranged by size:
2749 1614 1438
ApaI G_GGCC'C
Cuts at: 687 2392 687
Size: 1705 4096
ApaLI G'TGCA_C
Cuts at: 4639 4639
Size: 5801
ApoI r'AATT_y
Cuts at: 1553 1567 1597 2712 2805 5154 5410 1553
Size: 14 30 1115 93 2349 256 1944
Fragments arranged by size:
2349 1944 1115 256 93 30 14
AseI AT'TA_AT
Cuts at: 20 2620 20
Size: 2600 3201
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5801
AvaI C'yCGr_G
Cuts at: 1405 1920 2384 2821 1405
Size: 515 464 437 4385
Fragments arranged by size:
4385 515 464 437
AvrII C'CTAG_G
Cuts at: 961 961
Size: 5801
BamHI G'GATC_C
Cuts at: 2826 2826
Size: 5801
BanI G'GyrC_C
Cuts at: 1221 1471 1848 1894 2110 2817 3231 3479 3514
Size: 250 377 46 216 707 414 248 35
Cuts at: 3514 1221
Size: 3508
Fragments arranged by size:
3508 707 414 377 250 248 216 46
35
BanII G_rGCy'C
Cuts at: 667 687 1101 1388 1946 1985 2392 2675 2815
Size: 20 414 287 558 39 407 283 140
Cuts at: 2815 3845 667
Size: 1030 2623
Fragments arranged by size:
2623 1030 558 414 407 287 283 140
39 20
BbeI G_GCGC'C
Cuts at: 1225 3235 3483 1225
Size: 2010 248 3543
Fragments arranged by size:
3543 2010 248
BbsI GAAGACnn'nnnn_
Cuts at: 647 1067 647
Size: 420 5381
BciVI GTATCCnnnnn_n'
Cuts at: 3699 4755 5801 3699
Size: 1056 1046 3699
Fragments arranged by size:
3699 1056 1046
BclI T'GATC_A
Cuts at: 3321 3321
Size: 5801
BglI GCCn_nnn'nGGC
Cuts at: 1903 1903
Size: 5801
BglII A'GATC_T
Cuts at: 3316 5353 3316
Size: 2037 3764
BlpI GC'TnA_GC
Cuts at: 2913 2913
Size: 5801
Bme1580I G_kGCm'C
Cuts at: 687 2374 2392 3426 3519 4643 687
Size: 1687 18 1034 93 1124 1845
Fragments arranged by size:
1845 1687 1124 1034 93 18
BmgBI CAC'GTC
Cuts at: 525 902 525
Size: 377 5424
BmrI ACTGGGnnnn_n'
Cuts at: 1007 1425 1677 2094 2364 3013 3094 3429 5573
Size: 418 252 417 270 649 81 335 2144
Cuts at: 5573 1007
Size: 1235
Fragments arranged by size:
2144 1235 649 418 417 335 270 252
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1242 1500 1932 2757 4168 1242
Size: 258 432 825 1411 2875
Fragments arranged by size:
2875 1411 825 432 258
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 868 868
Size: 5801
Bpu10I CC'TnA_GC
Cuts at: 1212 1693 1987 1212
Size: 481 294 5026
Fragments arranged by size:
5026 481 294
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1177 2977 3856 4570 4868 1177
Size: 1800 879 714 298 2110
Fragments arranged by size:
2110 1800 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 796 2753 5505 796
Size: 1957 2752 1092
Fragments arranged by size:
2752 1957 1092
BsaAI yAC'GTr
Cuts at: 3154 3154
Size: 5801
BsaBI GATnn'nnATC
Cuts at: 6 3315 5352 6
Size: 3309 2037 455
Fragments arranged by size:
3309 2037 455
BsaHI Gr'CG_yC
Cuts at: 1222 3232 3480 4182 5678 1222
Size: 2010 248 702 1496 1345
Fragments arranged by size:
2010 1496 1345 702 248
BsaWI w'CCGG_w
Cuts at: 2990 3511 4189 4600 4747 5021 2990
Size: 521 678 411 147 274 3770
Fragments arranged by size:
3770 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 886 916 2042 2072 2201 2231 2671 2701 886
Size: 30 1126 30 129 30 440 30 3986
Fragments arranged by size:
3986 1126 440 129 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 391 1142 1908 2022 391
Size: 751 766 114 4170
Fragments arranged by size:
4170 766 751 114
BseYI C'CCAG_C
Cuts at: 130 270 2226 2407 3226 3984 4649 5057 130
Size: 140 1956 181 819 758 665 408 874
Fragments arranged by size:
1956 874 819 758 665 408 181 140
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 344 2144 2335 344
Size: 1800 191 3810
Fragments arranged by size:
3810 1800 191
BsiEI CG_ry'CG
Cuts at: 104 1892 1907 3343 3389 4619 104
Size: 1788 15 1436 46 1230 1286
Fragments arranged by size:
1788 1436 1286 1230 46 15
BsiHKAI G_wGCw'C
Cuts at: 667 1946 2675 2815 3593 3783 4643 667
Size: 1279 729 140 778 190 860 1825
Fragments arranged by size:
1825 1279 860 778 729 190 140
Bsp1286I G_dGCh'C
Cuts at: 667 687 1101 1388 1946 1985 2374 2392 2675
Size: 20 414 287 558 39 389 18 283
Cuts at: 2675 2815 3426 3519 3593 3783 3845 4643 667
Size: 140 611 93 74 190 62 798 1825
Fragments arranged by size:
1825 798 611 558 414 389 287 283
190 140 93 74 62 39 20 18
BspHI T'CATG_A
Cuts at: 4233 4233
Size: 5801
BspMI ACCTGCnnnn'nnnn_
Cuts at: 126 867 1874 2357 2851 3065 3367 3748 126
Size: 741 1007 483 494 214 302 381 2179
Fragments arranged by size:
2179 1007 741 494 483 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4093 4147 5631 5796 50
Size: 4043 54 1484 165 55
Fragments arranged by size:
4043 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3713 3713
Size: 5801
BsrFI r'CCGG_y
Cuts at: 1239 1887 1891 1902 3799 3980 1239
Size: 648 4 11 1897 181 3060
Fragments arranged by size:
3060 1897 648 181 11 4
BssSI C'ACGA_G
Cuts at: 695 2312 2674 4072 4780 695
Size: 1617 362 1398 708 1716
Fragments arranged by size:
1716 1617 1398 708 362
BstAPI GCAn_nnn'nTGC
Cuts at: 631 1478 3178 631
Size: 847 1700 3254
Fragments arranged by size:
3254 1700 847
BstBI TT'CG_AA
Cuts at: 4162 4162
Size: 5801
BstXI CCAn_nnnn'nTGG
Cuts at: 1101 1962 2325 1101
Size: 861 363 4577
Fragments arranged by size:
4577 861 363
BstYI r'GATC_y
Cuts at: 1 283 330 700 2826 3293 3316 3651 3897
Size: 282 47 370 2126 467 23 335 246
Cuts at: 3897 4203 4215 4301 5353 1
Size: 306 12 86 1052 449
Fragments arranged by size:
2126 1052 467 449 370 335 306 282
246 86 47 23 12
Bsu36I CC'TnA_GG
Cuts at: 1724 1724
Size: 5801
BtgI C'CryG_G
Cuts at: 1055 1445 1496 1992 2340 1055
Size: 390 51 496 348 4516
Fragments arranged by size:
4516 496 390 348 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3751 3932 4095 3751
Size: 181 163 5457
Fragments arranged by size:
5457 181 163
BtsI GCAGTG_nn'
Cuts at: 327 332 1405 3044 327
Size: 5 1073 1639 3084
Fragments arranged by size:
3084 1639 1073 5
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1112 1147 1112
Size: 35 5766
DraI TTT'AAA
Cuts at: 453 2800 453
Size: 2347 3454
DraIII CAC_nnn'GTG
Cuts at: 1478 1478
Size: 5801
DrdI GACnn_nn'nnGTC
Cuts at: 3507 4851 3507
Size: 1344 4457
EaeI y'GGCC_r
Cuts at: 1889 1904 2327 3386 3560 3951 3978 5323 1889
Size: 15 423 1059 174 391 27 1345 2367
Fragments arranged by size:
2367 1345 1059 423 391 174 27 15
EagI C'GGCC_G
Cuts at: 1889 1904 3386 1889
Size: 15 1482 4304
Fragments arranged by size:
4304 1482 15
EarI CTCTTCn'nnn_
Cuts at: 608 754 1708 3824 4034 608
Size: 146 954 2116 210 2375
Fragments arranged by size:
2375 2116 954 210 146
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 923 2014 2412 4009 4751 4897 5611 923
Size: 1091 398 1597 742 146 714 1113
Fragments arranged by size:
1597 1113 1091 742 714 398 146
EcoICRI GAG'CTC
Cuts at: 665 1944 2673 2813 665
Size: 1279 729 140 3653
Fragments arranged by size:
3653 1279 729 140
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 404 458 868 1367 2074 2792 3626 4058 4411
Size: 54 410 499 707 718 834 432 353
Cuts at: 4411 5073 404
Size: 662 1132
Fragments arranged by size:
1132 834 718 707 662 499 432 410
353 54
EcoO109I rG'GnC_Cy
Cuts at: 683 1303 1533 2388 2389 2940 683
Size: 620 230 855 1 551 3544
Fragments arranged by size:
3544 855 620 551 230 1
EcoRI G'AATT_C
Cuts at: 2712 2805 2712
Size: 93 5708
EcoRV GAT'ATC
Cuts at: 2487 2487
Size: 5801
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2535 3820 2535
Size: 1285 4516
FseI GG_CCGG'CC
Cuts at: 1906 1906
Size: 5801
FspI TGC'GCA
Cuts at: 3582 3582
Size: 5801
HaeII r_GCGC'y
Cuts at: 1225 3235 3483 4713 5484 1225
Size: 2010 248 1230 771 1542
Fragments arranged by size:
2010 1542 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 795 810 827 842 1111 1143 1246 1278 1405
Size: 15 17 15 269 32 103 32 127
Cuts at: 1405 1437 1638 1670 1825 1855 1857 1887 1918
Size: 32 201 32 155 30 2 30 31
Cuts at: 1918 1950 2201 2233 2750 2776 2782 2808 3643
Size: 32 251 32 517 26 6 26 835
Cuts at: 3643 3673 3675 3705 795
Size: 30 2 30 2891
Fragments arranged by size:
2891 835 517 269 251 201 155 127
103 32 32 32 32 32 32 31
30 30 30 30 26 26 17 15
15 6 2 2
HincII GTy'rAC
Cuts at: 2840 2840
Size: 5801
HindIII A'AGCT_T
Cuts at: 362 400 362
Size: 38 5763
KasI G'GCGC_C
Cuts at: 1221 3231 3479 1221
Size: 2010 248 3543
Fragments arranged by size:
3543 2010 248
KpnI G_GTAC'C
Cuts at: 2821 2821
Size: 5801
MluI A'CGCG_T
Cuts at: 5234 5234
Size: 5801
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2177 2553 4560 4744 2177
Size: 376 2007 184 3234
Fragments arranged by size:
3234 2007 376 184
MscI TGG'CCA
Cuts at: 3562 3562
Size: 5801
MslI CAynn'nnrTG
Cuts at: 1960 2323 3790 3917 1960
Size: 363 1467 127 3844
Fragments arranged by size:
3844 1467 363 127
MspA1I CmG'CkG
Cuts at: 126 1459 1818 2226 2912 3226 3586 4370 4615
Size: 1333 359 408 686 314 360 784 245
Cuts at: 4615 126
Size: 1312
Fragments arranged by size:
1333 1312 784 686 408 360 359 314
245
NaeI GCC'GGC
Cuts at: 1889 1904 3982 1889
Size: 15 2078 3708
Fragments arranged by size:
3708 2078 15
NarI GG'CG_CC
Cuts at: 1222 3232 3480 1222
Size: 2010 248 3543
Fragments arranged by size:
3543 2010 248
NcoI C'CATG_G
Cuts at: 1055 1496 1055
Size: 441 5360
NgoMIV G'CCGG_C
Cuts at: 1887 1902 3980 1887
Size: 15 2078 3708
Fragments arranged by size:
3708 2078 15
NspI r_CATG'y
Cuts at: 650 2686 2854 3354 4957 650
Size: 2036 168 500 1603 1494
Fragments arranged by size:
2036 1603 1494 500 168
PasI CC'CwG_GG
Cuts at: 1101 1101
Size: 5801
PciI A'CATG_T
Cuts at: 646 4953 646
Size: 4307 1494
PflMI CCAn_nnn'nTGG
Cuts at: 1400 1400
Size: 5801
PmeI GTTT'AAAC
Cuts at: 2800 2800
Size: 5801
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2671 2703 4247 4279 2671
Size: 32 1544 32 4193
Fragments arranged by size:
4193 1544 32 32
PpuMI rG'GwC_Cy
Cuts at: 1303 1303
Size: 5801
PspOMI G'GGCC_C
Cuts at: 683 2388 683
Size: 1705 4096
PspXI vC'TCGA_Gb
Cuts at: 1920 1920
Size: 5801
PstI C_TGCA'G
Cuts at: 639 1464 2848 3533 639
Size: 825 1384 685 2907
Fragments arranged by size:
2907 1384 825 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5801
PvuII CAG'CTG
Cuts at: 126 1459 1818 2226 3226 3586 126
Size: 1333 359 408 1000 360 2341
Fragments arranged by size:
2341 1333 1000 408 360 359
RsrII CG'GwC_CG
Cuts at: 3996 3996
Size: 5801
SacI G_AGCT'C
Cuts at: 667 1946 2675 2815 667
Size: 1279 729 140 3653
Fragments arranged by size:
3653 1279 729 140
SalI G'TCGA_C
Cuts at: 2838 2838
Size: 5801
SapI GCTCTTCn'nnn_
Cuts at: 608 754 1708 3824 4034 608
Size: 146 954 2116 210 2375
Fragments arranged by size:
2375 2116 954 210 146
SbfI CC_TGCA'GG
Cuts at: 2848 2848
Size: 5801
ScaI AGT'ACT
Cuts at: 1164 5318 1164
Size: 4154 1647
SfcI C'TryA_G
Cuts at: 33 635 1460 2586 2844 3529 4497 4688 33
Size: 602 825 1126 258 685 968 191 1146
Fragments arranged by size:
1146 1126 968 825 685 602 258 191
SfoI GGC'GCC
Cuts at: 1223 3233 3481 1223
Size: 2010 248 3543
Fragments arranged by size:
3543 2010 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5801
SmaI CCC'GGG
Cuts at: 2386 2823 2386
Size: 437 5364
SmlI C'TyrA_G
Cuts at: 1156 1920 2956 3871 4585 4847 1156
Size: 764 1036 915 714 262 2110
Fragments arranged by size:
2110 1036 915 764 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5801
SphI G_CATG'C
Cuts at: 2854 3354 2854
Size: 500 5301
SspI AAT'ATT
Cuts at: 2594 2594
Size: 5801
StyI C'CwwG_G
Cuts at: 926 961 1055 1496 1829 1880 2609 2935 3289
Size: 35 94 441 333 51 729 326 354
Cuts at: 3289 926
Size: 3438
Fragments arranged by size:
3438 729 441 354 333 326 94 51
35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 182 337 1734 3384 3517 3976 4185 182
Size: 155 1397 1650 133 459 209 1798
Fragments arranged by size:
1798 1650 1397 459 209 155 133
TatI w'GTAC_w
Cuts at: 1162 2300 5316 1162
Size: 1138 3016 1647
Fragments arranged by size:
3016 1647 1138
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 886 918 5318 5350 886
Size: 32 4400 32 1337
Fragments arranged by size:
4400 1337 32 32
Tth111I GACn'n_nGTC
Cuts at: 1066 3598 1066
Size: 2532 3269
XbaI T'CTAG_A
Cuts at: 1338 2832 1338
Size: 1494 4307
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 3296 114
Size: 3182 2619
XhoI C'TCGA_G
Cuts at: 1920 1920
Size: 5801
XmaI C'CCGG_G
Cuts at: 2384 2821 2384
Size: 437 5364
XmnI GAAnn'nnTTC
Cuts at: 401 401
Size: 5801
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PmeI
PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AleI AscI BaeI BbvCI
BcgI BfrBI BmtI BsiWI BsmI BsmBI BspEI BsrGI
BssHII BstEII BstZ17I ClaI EcoNI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PfoI PmlI
PshAI PsiI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI ZraI