Restriction map for
pF1KA0256
(Circular) (Six-base) MAPSORT of: pF1KA0256.seq Check: 5956 from: 1 to: 6281
pF1KA0256 6281 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1015 1015
Size: 6281
AccI GT'mk_AC
Cuts at: 424 3319 424
Size: 2895 3386
Acc65I G'GTAC_C
Cuts at: 2490 3297 2490
Size: 807 5474
AclI AA'CG_TT
Cuts at: 6121 6121
Size: 6281
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2594 2609 3049 4106 4538 5285 5553 2594
Size: 15 440 1057 432 747 268 3322
Fragments arranged by size:
3322 1057 747 440 432 268 15
AflIII A'CryG_T
Cuts at: 1454 3633 4737 5714 1454
Size: 2179 1104 977 2021
Fragments arranged by size:
2179 2021 1104 977
AgeI A'CCGG_T
Cuts at: 4669 4669
Size: 6281
AlwNI CAG_nnn'CTG
Cuts at: 721 2854 3221 3586 5153 721
Size: 2133 367 365 1567 1849
Fragments arranged by size:
2133 1849 1567 367 365
ApaLI G'TGCA_C
Cuts at: 5051 5051
Size: 6281
ApoI r'AATT_y
Cuts at: 2273 2889 3285 5634 5890 2273
Size: 616 396 2349 256 2664
Fragments arranged by size:
2664 2349 616 396 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6281
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6281
AvaI C'yCGr_G
Cuts at: 3301 3301
Size: 6281
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2906 2939 2906
Size: 33 6248
BamHI G'GATC_C
Cuts at: 1765 3306 1765
Size: 1541 4740
BanI G'GyrC_C
Cuts at: 2490 3135 3297 3711 3959 3994 2490
Size: 645 162 414 248 35 4777
Fragments arranged by size:
4777 645 414 248 162 35
BanII G_rGCy'C
Cuts at: 120 3295 4325 120
Size: 3175 1030 2076
Fragments arranged by size:
3175 2076 1030
BbeI G_GCGC'C
Cuts at: 3139 3715 3963 3139
Size: 576 248 5457
Fragments arranged by size:
5457 576 248
BbsI GAAGACnn'nnnn_
Cuts at: 570 3104 570
Size: 2534 3747
BbvCI CC'TCA_GC
Cuts at: 149 2462 2763 149
Size: 2313 301 3667
Fragments arranged by size:
3667 2313 301
BciVI GTATCCnnnnn_n'
Cuts at: 2673 4179 4940 6281 2673
Size: 1506 761 1341 2673
Fragments arranged by size:
2673 1506 1341 761
BclI T'GATC_A
Cuts at: 3801 3801
Size: 6281
BglII A'GATC_T
Cuts at: 2880 3796 5833 2880
Size: 916 2037 3328
Fragments arranged by size:
3328 2037 916
BlpI GC'TnA_GC
Cuts at: 401 3393 401
Size: 2992 3289
Bme1580I G_kGCm'C
Cuts at: 459 3906 3999 5055 459
Size: 3447 93 1056 1685
Fragments arranged by size:
3447 1685 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1850 2687 3493 3574 3909 6053 1850
Size: 837 806 81 335 2144 2078
Fragments arranged by size:
2144 2078 837 806 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2874 4648 2874
Size: 1774 4507
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1859 3169 1859
Size: 1310 4971
Bpu10I CC'TnA_GC
Cuts at: 149 1184 2462 2763 149
Size: 1035 1278 301 3667
Fragments arranged by size:
3667 1278 1035 301
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1334 3457 4336 4828 5126 1334
Size: 2123 879 492 298 2489
Fragments arranged by size:
2489 2123 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 802 3034 3039 5985 802
Size: 2232 5 2946 1098
Fragments arranged by size:
2946 2232 1098 5
BsaAI yAC'GTr
Cuts at: 3634 3634
Size: 6281
BsaBI GATnn'nnATC
Cuts at: 6 3795 5832 6
Size: 3789 2037 455
Fragments arranged by size:
3789 2037 455
BsaHI Gr'CG_yC
Cuts at: 3136 3712 3960 4662 6158 3136
Size: 576 248 702 1496 3259
Fragments arranged by size:
3259 1496 702 576 248
BsaWI w'CCGG_w
Cuts at: 3470 3991 4669 4943 5090 5501 3470
Size: 521 678 274 147 411 4250
Fragments arranged by size:
4250 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2422 2974 2977 2980 2422
Size: 552 3 3 5723
Fragments arranged by size:
5723 552 3 3
BseYI C'CCAG_C
Cuts at: 259 2776 3706 4464 5041 5537 259
Size: 2517 930 758 577 496 1003
Fragments arranged by size:
2517 1003 930 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 1043 1456 3025 310
Size: 733 413 1569 3566
Fragments arranged by size:
3566 1569 733 413
BsiEI CG_ry'CG
Cuts at: 104 3823 3869 5077 104
Size: 3719 46 1208 1308
Fragments arranged by size:
3719 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 3245 3295 4073 4263 5055 3245
Size: 50 778 190 792 4471
Fragments arranged by size:
4471 792 778 190 50
BsmI GAATG_Cn'
Cuts at: 550 550
Size: 6281
BsmBI CGTCTCn'nnnn_
Cuts at: 2112 2112
Size: 6281
Bsp1286I G_dGCh'C
Cuts at: 120 459 3245 3295 3906 3999 4073 4263 4325
Size: 339 2786 50 611 93 74 190 62
Cuts at: 4325 5055 120
Size: 730 1346
Fragments arranged by size:
2786 1346 730 611 339 190 93 74
62 50
BspEI T'CCGG_A
Cuts at: 5501 5501
Size: 6281
BspHI T'CATG_A
Cuts at: 2982 5457 2982
Size: 2475 3806
BspMI ACCTGCnnnn'nnnn_
Cuts at: 706 1015 3331 3545 3847 4228 706
Size: 309 2316 214 302 381 2759
Fragments arranged by size:
2759 2316 381 309 302 214
BsrBI CCG'CTC
Cuts at: 50 4573 4627 6111 6276 50
Size: 4523 54 1484 165 55
Fragments arranged by size:
4523 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1493 1628 2450 4193 1493
Size: 135 822 1743 3581
Fragments arranged by size:
3581 1743 822 135
BsrFI r'CCGG_y
Cuts at: 4279 4460 4669 4279
Size: 181 209 5891
Fragments arranged by size:
5891 209 181
BssSI C'ACGA_G
Cuts at: 4552 4910 4552
Size: 358 5923
BstAPI GCAn_nnn'nTGC
Cuts at: 454 1339 2533 3178 3658 454
Size: 885 1194 645 480 3077
Fragments arranged by size:
3077 1194 885 645 480
BstBI TT'CG_AA
Cuts at: 4642 4642
Size: 6281
BstYI r'GATC_y
Cuts at: 1 1765 2094 2880 3306 3773 3796 4131 4377
Size: 1764 329 786 426 467 23 335 246
Cuts at: 4377 5389 5475 5487 5833 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1764 1012 786 467 449 426 346 335
329 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 425 425
Size: 6281
Bsu36I CC'TnA_GG
Cuts at: 3140 3140
Size: 6281
BtgI C'CryG_G
Cuts at: 107 122 2255 107
Size: 15 2133 4133
Fragments arranged by size:
4133 2133 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4231 4412 4575 4231
Size: 181 163 5937
Fragments arranged by size:
5937 181 163
BtsI GCAGTG_nn'
Cuts at: 812 2556 3524 812
Size: 1744 968 3569
Fragments arranged by size:
3569 1744 968
DraI TTT'AAA
Cuts at: 1712 3280 1712
Size: 1568 4713
DraIII CAC_nnn'GTG
Cuts at: 454 454
Size: 6281
DrdI GACnn_nn'nnGTC
Cuts at: 1911 3987 4845 1911
Size: 2076 858 3347
Fragments arranged by size:
3347 2076 858
EaeI y'GGCC_r
Cuts at: 3866 4040 4431 4458 5803 3866
Size: 174 391 27 1345 4344
Fragments arranged by size:
4344 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3866 3866
Size: 6281
EarI CTCTTCn'nnn_
Cuts at: 172 846 2051 3116 4304 4514 172
Size: 674 1205 1065 1188 210 1939
Fragments arranged by size:
1939 1205 1188 1065 674 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4489 4799 4945 6091 4489
Size: 310 146 1146 4679
Fragments arranged by size:
4679 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3293 3293
Size: 6281
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1859 2594 2609 3049 3169 4106 4538 5285 5553
Size: 735 15 440 120 937 432 747 268
Cuts at: 5553 1859
Size: 2587
Fragments arranged by size:
2587 937 747 735 440 432 268 120
15
EcoNI CCTnn'n_nnAGG
Cuts at: 962 962
Size: 6281
EcoO109I rG'GnC_Cy
Cuts at: 718 3420 718
Size: 2702 3579
EcoRI G'AATT_C
Cuts at: 2889 3285 2889
Size: 396 5885
EcoRV GAT'ATC
Cuts at: 786 786
Size: 6281
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1755 4300 1755
Size: 2545 3736
FspI TGC'GCA
Cuts at: 4062 4062
Size: 6281
HaeII r_GCGC'y
Cuts at: 1740 3139 3715 3963 4985 5964 1740
Size: 1399 576 248 1022 979 2057
Fragments arranged by size:
2057 1399 1022 979 576 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 611 643 715 747 763 795 984 1016 4123
Size: 32 72 32 16 32 189 32 3107
Cuts at: 4123 4153 4155 4185 611
Size: 30 2 30 2707
Fragments arranged by size:
3107 2707 189 72 32 32 32 32
30 30 16 2
HincII GTy'rAC
Cuts at: 3320 3320
Size: 6281
HindIII A'AGCT_T
Cuts at: 1322 1394 1322
Size: 72 6209
KasI G'GCGC_C
Cuts at: 3135 3711 3959 3135
Size: 576 248 5457
Fragments arranged by size:
5457 576 248
KpnI G_GTAC'C
Cuts at: 2494 3301 2494
Size: 807 5474
MfeI C'AATT_G
Cuts at: 2720 2720
Size: 6281
MluI A'CGCG_T
Cuts at: 5714 5714
Size: 6281
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 524 4952 5136 524
Size: 4428 184 1669
Fragments arranged by size:
4428 1669 184
MscI TGG'CCA
Cuts at: 4042 4042
Size: 6281
MslI CAynn'nnrTG
Cuts at: 1017 4270 4397 1017
Size: 3253 127 2901
Fragments arranged by size:
3253 2901 127
MspA1I CmG'CkG
Cuts at: 148 438 675 1411 3392 3706 4066 5079 5324
Size: 290 237 736 1981 314 360 1013 245
Cuts at: 5324 148
Size: 1105
Fragments arranged by size:
1981 1105 1013 736 360 314 290 245
237
NaeI GCC'GGC
Cuts at: 4462 4462
Size: 6281
NarI GG'CG_CC
Cuts at: 3136 3712 3960 3136
Size: 576 248 5457
Fragments arranged by size:
5457 576 248
NcoI C'CATG_G
Cuts at: 107 2255 107
Size: 2148 4133
NdeI CA'TA_TG
Cuts at: 2156 2156
Size: 6281
NgoMIV G'CCGG_C
Cuts at: 4460 4460
Size: 6281
NspI r_CATG'y
Cuts at: 1458 1854 2934 3334 3834 4741 1458
Size: 396 1080 400 500 907 2998
Fragments arranged by size:
2998 1080 907 500 400 396
PciI A'CATG_T
Cuts at: 1454 4737 1454
Size: 3283 2998
PflMI CCAn_nnn'nTGG
Cuts at: 465 465
Size: 6281
PfoI T'CCnGG_A
Cuts at: 2072 2072
Size: 6281
PmeI GTTT'AAAC
Cuts at: 3280 3280
Size: 6281
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5420 5452 5420
Size: 32 6249
PpuMI rG'GwC_Cy
Cuts at: 718 718
Size: 6281
PshAI GACnn'nnGTC
Cuts at: 1521 1521
Size: 6281
PsiI TTA'TAA
Cuts at: 2185 2185
Size: 6281
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1879 1911 1879
Size: 32 6249
PstI C_TGCA'G
Cuts at: 934 963 1545 2529 2551 3226 3328 4013 934
Size: 29 582 984 22 675 102 685 3202
Fragments arranged by size:
3202 984 685 675 582 102 29 22
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6281
PvuII CAG'CTG
Cuts at: 148 438 1411 3706 4066 148
Size: 290 973 2295 360 2363
Fragments arranged by size:
2363 2295 973 360 290
RsrII CG'GwC_CG
Cuts at: 4476 4476
Size: 6281
SacI G_AGCT'C
Cuts at: 3295 3295
Size: 6281
SalI G'TCGA_C
Cuts at: 3318 3318
Size: 6281
SapI GCTCTTCn'nnn_
Cuts at: 846 4304 4514 846
Size: 3458 210 2613
Fragments arranged by size:
3458 2613 210
SbfI CC_TGCA'GG
Cuts at: 3328 3328
Size: 6281
ScaI AGT'ACT
Cuts at: 2757 5798 2757
Size: 3041 3240
SfcI C'TryA_G
Cuts at: 33 416 930 959 1541 2525 2547 2747 2996
Size: 383 514 29 582 984 22 200 249
Cuts at: 2996 3222 3324 4009 5002 5193 33
Size: 226 102 685 993 191 1121
Fragments arranged by size:
1121 993 984 685 582 514 383 249
226 200 191 102 29 22
SfoI GGC'GCC
Cuts at: 3137 3713 3961 3137
Size: 576 248 5457
Fragments arranged by size:
5457 576 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6281
SmaI CCC'GGG
Cuts at: 3303 3303
Size: 6281
SmlI C'TyrA_G
Cuts at: 1349 3436 4351 4843 5105 1349
Size: 2087 915 492 262 2525
Fragments arranged by size:
2525 2087 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6281
SphI G_CATG'C
Cuts at: 1854 2934 3334 3834 1854
Size: 1080 400 500 4301
Fragments arranged by size:
4301 1080 500 400
SspI AAT'ATT
Cuts at: 1197 1197
Size: 6281
StyI C'CwwG_G
Cuts at: 107 836 1075 2255 2292 3415 3769 107
Size: 729 239 1180 37 1123 354 2619
Fragments arranged by size:
2619 1180 1123 729 354 239 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 128 3864 3997 4456 4665 128
Size: 3736 133 459 209 1744
Fragments arranged by size:
3736 1744 459 209 133
TatI w'GTAC_w
Cuts at: 2755 2845 5796 2755
Size: 90 2951 3240
Fragments arranged by size:
3240 2951 90
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 540 572 1275 1307 2303 2335 3150 3182 5798
Size: 32 703 32 996 32 815 32 2616
Cuts at: 5798 5830 540
Size: 32 991
Fragments arranged by size:
2616 996 991 815 703 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 4078 4078
Size: 6281
XbaI T'CTAG_A
Cuts at: 1102 3312 1102
Size: 2210 4071
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1002 1837 2218 3776 1002
Size: 835 381 1558 3507
Fragments arranged by size:
3507 1558 835 381
XmaI C'CCGG_G
Cuts at: 3301 3301
Size: 6281
XmnI GAAnn'nnTTC
Cuts at: 231 1134 3062 231
Size: 903 1928 3450
Fragments arranged by size:
3450 1928 903
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BcgI BfrBI BglI BmgBI BmtI BsaXI
BsiWI BsrGI BssHII BstEII BstXI ClaI CspCI FseI
FspAI HpaI NheI NotI NruI NsiI PacI PasI
PmlI PspOMI PspXI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI