Restriction map for
pF1KA0260
(Circular) (Six-base) MAPSORT of: pF1KA0260.seq Check: 2797 from: 1 to: 4178
pF1KA0260 4178 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AatII G_ACGT'C
Cuts at: 129 129
Size: 4178
AccI GT'mk_AC
Cuts at: 1216 1216
Size: 4178
Acc65I G'GTAC_C
Cuts at: 1194 1194
Size: 4178
AclI AA'CG_TT
Cuts at: 4018 4018
Size: 4178
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 575 1127 2003 2435 3182 3450 250
Size: 325 552 876 432 747 268 978
Fragments arranged by size:
978 876 747 552 432 325 268
AflIII A'CryG_T
Cuts at: 1530 2634 3611 1530
Size: 1104 977 2097
Fragments arranged by size:
2097 1104 977
AgeI A'CCGG_T
Cuts at: 2566 2566
Size: 4178
AlwNI CAG_nnn'CTG
Cuts at: 1483 3050 1483
Size: 1567 2611
ApaLI G'TGCA_C
Cuts at: 2948 2948
Size: 4178
ApoI r'AATT_y
Cuts at: 1182 3531 3787 1182
Size: 2349 256 1573
Fragments arranged by size:
2349 1573 256
AseI AT'TA_AT
Cuts at: 20 991 20
Size: 971 3207
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4178
AvaI C'yCGr_G
Cuts at: 137 1198 137
Size: 1061 3117
BamHI G'GATC_C
Cuts at: 1203 1203
Size: 4178
BanI G'GyrC_C
Cuts at: 1194 1608 1856 1891 1194
Size: 414 248 35 3481
Fragments arranged by size:
3481 414 248 35
BanII G_rGCy'C
Cuts at: 265 1192 2222 265
Size: 927 1030 2221
Fragments arranged by size:
2221 1030 927
BbeI G_GCGC'C
Cuts at: 1612 1860 1612
Size: 248 3930
BbsI GAAGACnn'nnnn_
Cuts at: 580 580
Size: 4178
BciVI GTATCCnnnnn_n'
Cuts at: 2076 2837 4178 2076
Size: 761 1341 2076
Fragments arranged by size:
2076 1341 761
BclI T'GATC_A
Cuts at: 1698 1698
Size: 4178
BglI GCCn_nnn'nGGC
Cuts at: 358 358
Size: 4178
BglII A'GATC_T
Cuts at: 1693 3730 1693
Size: 2037 2141
BlpI GC'TnA_GC
Cuts at: 1117 1290 1117
Size: 173 4005
Bme1580I G_kGCm'C
Cuts at: 1803 1896 2952 1803
Size: 93 1056 3029
Fragments arranged by size:
3029 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1390 1471 1806 3950 1390
Size: 81 335 2144 1618
Fragments arranged by size:
2144 1618 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 583 2545 583
Size: 1962 2216
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 1354 2233 2725 3023 553
Size: 801 879 492 298 1708
Fragments arranged by size:
1708 879 801 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3882 3882
Size: 4178
BsaAI yAC'GTr
Cuts at: 720 1037 1531 720
Size: 317 494 3367
Fragments arranged by size:
3367 494 317
BsaBI GATnn'nnATC
Cuts at: 6 1692 3729 6
Size: 1686 2037 455
Fragments arranged by size:
2037 1686 455
BsaHI Gr'CG_yC
Cuts at: 126 202 1609 1857 2559 4055 126
Size: 76 1407 248 702 1496 249
Fragments arranged by size:
1496 1407 702 249 248 76
BsaWI w'CCGG_w
Cuts at: 1367 1888 2566 2840 2987 3398 1367
Size: 521 678 274 147 411 2147
Fragments arranged by size:
2147 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 614 644 1157 1187 614
Size: 30 513 30 3605
Fragments arranged by size:
3605 513 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 199 202 309 199
Size: 3 107 4068
Fragments arranged by size:
4068 107 3
BseYI C'CCAG_C
Cuts at: 192 747 854 1072 1603 2361 2938 3434 192
Size: 555 107 218 531 758 577 496 936
Fragments arranged by size:
936 758 577 555 531 496 218 107
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 918 918
Size: 4178
BsiEI CG_ry'CG
Cuts at: 104 209 1720 1766 2974 104
Size: 105 1511 46 1208 1308
Fragments arranged by size:
1511 1308 1208 105 46
BsiHKAI G_wGCw'C
Cuts at: 265 299 1192 1970 2160 2952 265
Size: 34 893 778 190 792 1491
Fragments arranged by size:
1491 893 792 778 190 34
Bsp1286I G_dGCh'C
Cuts at: 265 299 1192 1803 1896 1970 2160 2222 2952
Size: 34 893 611 93 74 190 62 730
Cuts at: 2952 265
Size: 1491
Fragments arranged by size:
1491 893 730 611 190 93 74 62
34
BspEI T'CCGG_A
Cuts at: 3398 3398
Size: 4178
BspHI T'CATG_A
Cuts at: 785 3354 785
Size: 2569 1609
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1228 1442 1744 2125 1228
Size: 214 302 381 3281
Fragments arranged by size:
3281 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2470 2524 4008 4173 50
Size: 2420 54 1484 165 55
Fragments arranged by size:
2420 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 617 806 1067 2090 617
Size: 189 261 1023 2705
Fragments arranged by size:
2705 1023 261 189
BsrFI r'CCGG_y
Cuts at: 244 2176 2357 2566 244
Size: 1932 181 209 1856
Fragments arranged by size:
1932 1856 209 181
BssSI C'ACGA_G
Cuts at: 2449 2807 2449
Size: 358 3820
BstAPI GCAn_nnn'nTGC
Cuts at: 712 1555 712
Size: 843 3335
BstBI TT'CG_AA
Cuts at: 2539 2539
Size: 4178
BstYI r'GATC_y
Cuts at: 1 1203 1670 1693 2028 2274 3286 3372 3384
Size: 1202 467 23 335 246 1012 86 12
Cuts at: 3384 3730 1
Size: 346 449
Fragments arranged by size:
1202 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4178
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2128 2309 2472 2128
Size: 181 163 3834
Fragments arranged by size:
3834 181 163
BtsI GCAGTG_nn'
Cuts at: 1175 1421 1175
Size: 246 3932
DraI TTT'AAA
Cuts at: 1058 1177 1058
Size: 119 4059
DraIII CAC_nnn'GTG
Cuts at: 328 328
Size: 4178
DrdI GACnn_nn'nnGTC
Cuts at: 1884 2742 1884
Size: 858 3320
EaeI y'GGCC_r
Cuts at: 206 239 339 350 803 1763 1937 2328 2355
Size: 33 100 11 453 960 174 391 27
Cuts at: 2355 3700 206
Size: 1345 684
Fragments arranged by size:
1345 960 684 453 391 174 100 33
27 11
EagI C'GGCC_G
Cuts at: 206 1763 206
Size: 1557 2621
EarI CTCTTCn'nnn_
Cuts at: 1103 2201 2411 1103
Size: 1098 210 2870
Fragments arranged by size:
2870 1098 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 2386 2696 2842 3988 127
Size: 2259 310 146 1146 317
Fragments arranged by size:
2259 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 263 1190 263
Size: 927 3251
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 575 1127 2003 2435 3182 3450 250
Size: 325 552 876 432 747 268 978
Fragments arranged by size:
978 876 747 552 432 325 268
EcoO109I rG'GnC_Cy
Cuts at: 1317 1317
Size: 4178
EcoRI G'AATT_C
Cuts at: 1182 1182
Size: 4178
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2197 2197
Size: 4178
FspI TGC'GCA
Cuts at: 1959 1959
Size: 4178
HaeII r_GCGC'y
Cuts at: 385 1612 1860 2882 3861 385
Size: 1227 248 1022 979 702
Fragments arranged by size:
1227 1022 979 702 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2020 2050 2052 2082 2020
Size: 30 2 30 4116
Fragments arranged by size:
4116 30 30 2
HincII GTy'rAC
Cuts at: 1217 1217
Size: 4178
KasI G'GCGC_C
Cuts at: 1608 1856 1608
Size: 248 3930
KpnI G_GTAC'C
Cuts at: 1198 1198
Size: 4178
MluI A'CGCG_T
Cuts at: 3611 3611
Size: 4178
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 312 2849 3033 312
Size: 2537 184 1457
Fragments arranged by size:
2537 1457 184
MscI TGG'CCA
Cuts at: 341 352 805 1939 341
Size: 11 453 1134 2580
Fragments arranged by size:
2580 1134 453 11
MslI CAynn'nnrTG
Cuts at: 2167 2294 2167
Size: 127 4051
MspA1I CmG'CkG
Cuts at: 1116 1289 1603 1963 2976 3221 1116
Size: 173 314 360 1013 245 2073
Fragments arranged by size:
2073 1013 360 314 245 173
NaeI GCC'GGC
Cuts at: 246 2359 246
Size: 2113 2065
NarI GG'CG_CC
Cuts at: 1609 1857 1609
Size: 248 3930
NcoI C'CATG_G
Cuts at: 107 107
Size: 4178
NgoMIV G'CCGG_C
Cuts at: 244 2357 244
Size: 2113 2065
NspI r_CATG'y
Cuts at: 136 1231 1731 2638 136
Size: 1095 500 907 1676
Fragments arranged by size:
1676 1095 907 500
PciI A'CATG_T
Cuts at: 2634 2634
Size: 4178
PflMI CCAn_nnn'nTGG
Cuts at: 348 348
Size: 4178
PmeI GTTT'AAAC
Cuts at: 1177 1177
Size: 4178
PmlI CAC'GTG
Cuts at: 1037 1037
Size: 4178
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3317 3349 3317
Size: 32 4146
PstI C_TGCA'G
Cuts at: 881 1225 1910 881
Size: 344 685 3149
Fragments arranged by size:
3149 685 344
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4178
PvuII CAG'CTG
Cuts at: 1116 1603 1963 1116
Size: 487 360 3331
Fragments arranged by size:
3331 487 360
RsrII CG'GwC_CG
Cuts at: 2373 2373
Size: 4178
SacI G_AGCT'C
Cuts at: 265 1192 265
Size: 927 3251
SalI G'TCGA_C
Cuts at: 1215 1215
Size: 4178
SapI GCTCTTCn'nnn_
Cuts at: 1103 2201 2411 1103
Size: 1098 210 2870
Fragments arranged by size:
2870 1098 210
SbfI CC_TGCA'GG
Cuts at: 1225 1225
Size: 4178
ScaI AGT'ACT
Cuts at: 929 3695 929
Size: 2766 1412
SfcI C'TryA_G
Cuts at: 33 877 1221 1906 2899 3090 33
Size: 844 344 685 993 191 1121
Fragments arranged by size:
1121 993 844 685 344 191
SfoI GGC'GCC
Cuts at: 1610 1858 1610
Size: 248 3930
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4178
SmaI CCC'GGG
Cuts at: 1200 1200
Size: 4178
SmlI C'TyrA_G
Cuts at: 568 1333 2248 2740 3002 568
Size: 765 915 492 262 1744
Fragments arranged by size:
1744 915 765 492 262
SnaBI TAC'GTA
Cuts at: 720 720
Size: 4178
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4178
SphI G_CATG'C
Cuts at: 136 1231 1731 136
Size: 1095 500 2583
Fragments arranged by size:
2583 1095 500
StyI C'CwwG_G
Cuts at: 107 1312 1666 107
Size: 1205 354 2619
Fragments arranged by size:
2619 1205 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 360 1761 1894 2353 2562 360
Size: 1401 133 459 209 1976
Fragments arranged by size:
1976 1401 459 209 133
TatI w'GTAC_w
Cuts at: 927 3693 927
Size: 2766 1412
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3695 3727 3695
Size: 32 4146
Tth111I GACn'n_nGTC
Cuts at: 1975 1975
Size: 4178
XbaI T'CTAG_A
Cuts at: 1209 1209
Size: 4178
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 645 1673 645
Size: 1028 3150
XmaI C'CCGG_G
Cuts at: 1198 1198
Size: 4178
ZraI GAC'GTC
Cuts at: 127 127
Size: 4178
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PmeI PmlI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
BpmI Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SrfI
SspI StuI SwaI XhoI XmnI