(Circular) (Six-base) MAPSORT of: pF1KA0260.seq Check: 2797 from: 1 to: 4178 pF1KA0260 4178 bp With 182 enzymes: SgfI * June 20, 2008 10:19 .. AatII G_ACGT'C Cuts at: 129 129 Size: 4178 AccI GT'mk_AC Cuts at: 1216 1216 Size: 4178 Acc65I G'GTAC_C Cuts at: 1194 1194 Size: 4178 AclI AA'CG_TT Cuts at: 4018 4018 Size: 4178 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 250 575 1127 2003 2435 3182 3450 250 Size: 325 552 876 432 747 268 978 Fragments arranged by size: 978 876 747 552 432 325 268 AflIII A'CryG_T Cuts at: 1530 2634 3611 1530 Size: 1104 977 2097 Fragments arranged by size: 2097 1104 977 AgeI A'CCGG_T Cuts at: 2566 2566 Size: 4178 AlwNI CAG_nnn'CTG Cuts at: 1483 3050 1483 Size: 1567 2611 ApaLI G'TGCA_C Cuts at: 2948 2948 Size: 4178 ApoI r'AATT_y Cuts at: 1182 3531 3787 1182 Size: 2349 256 1573 Fragments arranged by size: 2349 1573 256 AseI AT'TA_AT Cuts at: 20 991 20 Size: 971 3207 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4178 AvaI C'yCGr_G Cuts at: 137 1198 137 Size: 1061 3117 BamHI G'GATC_C Cuts at: 1203 1203 Size: 4178 BanI G'GyrC_C Cuts at: 1194 1608 1856 1891 1194 Size: 414 248 35 3481 Fragments arranged by size: 3481 414 248 35 BanII G_rGCy'C Cuts at: 265 1192 2222 265 Size: 927 1030 2221 Fragments arranged by size: 2221 1030 927 BbeI G_GCGC'C Cuts at: 1612 1860 1612 Size: 248 3930 BbsI GAAGACnn'nnnn_ Cuts at: 580 580 Size: 4178 BciVI GTATCCnnnnn_n' Cuts at: 2076 2837 4178 2076 Size: 761 1341 2076 Fragments arranged by size: 2076 1341 761 BclI T'GATC_A Cuts at: 1698 1698 Size: 4178 BglI GCCn_nnn'nGGC Cuts at: 358 358 Size: 4178 BglII A'GATC_T Cuts at: 1693 3730 1693 Size: 2037 2141 BlpI GC'TnA_GC Cuts at: 1117 1290 1117 Size: 173 4005 Bme1580I G_kGCm'C Cuts at: 1803 1896 2952 1803 Size: 93 1056 3029 Fragments arranged by size: 3029 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1390 1471 1806 3950 1390 Size: 81 335 2144 1618 Fragments arranged by size: 2144 1618 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 583 2545 583 Size: 1962 2216 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 553 1354 2233 2725 3023 553 Size: 801 879 492 298 1708 Fragments arranged by size: 1708 879 801 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3882 3882 Size: 4178 BsaAI yAC'GTr Cuts at: 720 1037 1531 720 Size: 317 494 3367 Fragments arranged by size: 3367 494 317 BsaBI GATnn'nnATC Cuts at: 6 1692 3729 6 Size: 1686 2037 455 Fragments arranged by size: 2037 1686 455 BsaHI Gr'CG_yC Cuts at: 126 202 1609 1857 2559 4055 126 Size: 76 1407 248 702 1496 249 Fragments arranged by size: 1496 1407 702 249 248 76 BsaWI w'CCGG_w Cuts at: 1367 1888 2566 2840 2987 3398 1367 Size: 521 678 274 147 411 2147 Fragments arranged by size: 2147 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 614 644 1157 1187 614 Size: 30 513 30 3605 Fragments arranged by size: 3605 513 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 199 202 309 199 Size: 3 107 4068 Fragments arranged by size: 4068 107 3 BseYI C'CCAG_C Cuts at: 192 747 854 1072 1603 2361 2938 3434 192 Size: 555 107 218 531 758 577 496 936 Fragments arranged by size: 936 758 577 555 531 496 218 107 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 918 918 Size: 4178 BsiEI CG_ry'CG Cuts at: 104 209 1720 1766 2974 104 Size: 105 1511 46 1208 1308 Fragments arranged by size: 1511 1308 1208 105 46 BsiHKAI G_wGCw'C Cuts at: 265 299 1192 1970 2160 2952 265 Size: 34 893 778 190 792 1491 Fragments arranged by size: 1491 893 792 778 190 34 Bsp1286I G_dGCh'C Cuts at: 265 299 1192 1803 1896 1970 2160 2222 2952 Size: 34 893 611 93 74 190 62 730 Cuts at: 2952 265 Size: 1491 Fragments arranged by size: 1491 893 730 611 190 93 74 62 34 BspEI T'CCGG_A Cuts at: 3398 3398 Size: 4178 BspHI T'CATG_A Cuts at: 785 3354 785 Size: 2569 1609 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1228 1442 1744 2125 1228 Size: 214 302 381 3281 Fragments arranged by size: 3281 381 302 214 BsrBI CCG'CTC Cuts at: 50 2470 2524 4008 4173 50 Size: 2420 54 1484 165 55 Fragments arranged by size: 2420 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 617 806 1067 2090 617 Size: 189 261 1023 2705 Fragments arranged by size: 2705 1023 261 189 BsrFI r'CCGG_y Cuts at: 244 2176 2357 2566 244 Size: 1932 181 209 1856 Fragments arranged by size: 1932 1856 209 181 BssSI C'ACGA_G Cuts at: 2449 2807 2449 Size: 358 3820 BstAPI GCAn_nnn'nTGC Cuts at: 712 1555 712 Size: 843 3335 BstBI TT'CG_AA Cuts at: 2539 2539 Size: 4178 BstYI r'GATC_y Cuts at: 1 1203 1670 1693 2028 2274 3286 3372 3384 Size: 1202 467 23 335 246 1012 86 12 Cuts at: 3384 3730 1 Size: 346 449 Fragments arranged by size: 1202 1012 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 4178 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2128 2309 2472 2128 Size: 181 163 3834 Fragments arranged by size: 3834 181 163 BtsI GCAGTG_nn' Cuts at: 1175 1421 1175 Size: 246 3932 DraI TTT'AAA Cuts at: 1058 1177 1058 Size: 119 4059 DraIII CAC_nnn'GTG Cuts at: 328 328 Size: 4178 DrdI GACnn_nn'nnGTC Cuts at: 1884 2742 1884 Size: 858 3320 EaeI y'GGCC_r Cuts at: 206 239 339 350 803 1763 1937 2328 2355 Size: 33 100 11 453 960 174 391 27 Cuts at: 2355 3700 206 Size: 1345 684 Fragments arranged by size: 1345 960 684 453 391 174 100 33 27 11 EagI C'GGCC_G Cuts at: 206 1763 206 Size: 1557 2621 EarI CTCTTCn'nnn_ Cuts at: 1103 2201 2411 1103 Size: 1098 210 2870 Fragments arranged by size: 2870 1098 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 127 2386 2696 2842 3988 127 Size: 2259 310 146 1146 317 Fragments arranged by size: 2259 1146 317 310 146 EcoICRI GAG'CTC Cuts at: 263 1190 263 Size: 927 3251 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 250 575 1127 2003 2435 3182 3450 250 Size: 325 552 876 432 747 268 978 Fragments arranged by size: 978 876 747 552 432 325 268 EcoO109I rG'GnC_Cy Cuts at: 1317 1317 Size: 4178 EcoRI G'AATT_C Cuts at: 1182 1182 Size: 4178 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2197 2197 Size: 4178 FspI TGC'GCA Cuts at: 1959 1959 Size: 4178 HaeII r_GCGC'y Cuts at: 385 1612 1860 2882 3861 385 Size: 1227 248 1022 979 702 Fragments arranged by size: 1227 1022 979 702 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 2020 2050 2052 2082 2020 Size: 30 2 30 4116 Fragments arranged by size: 4116 30 30 2 HincII GTy'rAC Cuts at: 1217 1217 Size: 4178 KasI G'GCGC_C Cuts at: 1608 1856 1608 Size: 248 3930 KpnI G_GTAC'C Cuts at: 1198 1198 Size: 4178 MluI A'CGCG_T Cuts at: 3611 3611 Size: 4178 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 312 2849 3033 312 Size: 2537 184 1457 Fragments arranged by size: 2537 1457 184 MscI TGG'CCA Cuts at: 341 352 805 1939 341 Size: 11 453 1134 2580 Fragments arranged by size: 2580 1134 453 11 MslI CAynn'nnrTG Cuts at: 2167 2294 2167 Size: 127 4051 MspA1I CmG'CkG Cuts at: 1116 1289 1603 1963 2976 3221 1116 Size: 173 314 360 1013 245 2073 Fragments arranged by size: 2073 1013 360 314 245 173 NaeI GCC'GGC Cuts at: 246 2359 246 Size: 2113 2065 NarI GG'CG_CC Cuts at: 1609 1857 1609 Size: 248 3930 NcoI C'CATG_G Cuts at: 107 107 Size: 4178 NgoMIV G'CCGG_C Cuts at: 244 2357 244 Size: 2113 2065 NspI r_CATG'y Cuts at: 136 1231 1731 2638 136 Size: 1095 500 907 1676 Fragments arranged by size: 1676 1095 907 500 PciI A'CATG_T Cuts at: 2634 2634 Size: 4178 PflMI CCAn_nnn'nTGG Cuts at: 348 348 Size: 4178 PmeI GTTT'AAAC Cuts at: 1177 1177 Size: 4178 PmlI CAC'GTG Cuts at: 1037 1037 Size: 4178 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3317 3349 3317 Size: 32 4146 PstI C_TGCA'G Cuts at: 881 1225 1910 881 Size: 344 685 3149 Fragments arranged by size: 3149 685 344 PvuI CG_AT'CG Cuts at: 104 104 Size: 4178 PvuII CAG'CTG Cuts at: 1116 1603 1963 1116 Size: 487 360 3331 Fragments arranged by size: 3331 487 360 RsrII CG'GwC_CG Cuts at: 2373 2373 Size: 4178 SacI G_AGCT'C Cuts at: 265 1192 265 Size: 927 3251 SalI G'TCGA_C Cuts at: 1215 1215 Size: 4178 SapI GCTCTTCn'nnn_ Cuts at: 1103 2201 2411 1103 Size: 1098 210 2870 Fragments arranged by size: 2870 1098 210 SbfI CC_TGCA'GG Cuts at: 1225 1225 Size: 4178 ScaI AGT'ACT Cuts at: 929 3695 929 Size: 2766 1412 SfcI C'TryA_G Cuts at: 33 877 1221 1906 2899 3090 33 Size: 844 344 685 993 191 1121 Fragments arranged by size: 1121 993 844 685 344 191 SfoI GGC'GCC Cuts at: 1610 1858 1610 Size: 248 3930 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4178 SmaI CCC'GGG Cuts at: 1200 1200 Size: 4178 SmlI C'TyrA_G Cuts at: 568 1333 2248 2740 3002 568 Size: 765 915 492 262 1744 Fragments arranged by size: 1744 915 765 492 262 SnaBI TAC'GTA Cuts at: 720 720 Size: 4178 SpeI A'CTAG_T Cuts at: 67 67 Size: 4178 SphI G_CATG'C Cuts at: 136 1231 1731 136 Size: 1095 500 2583 Fragments arranged by size: 2583 1095 500 StyI C'CwwG_G Cuts at: 107 1312 1666 107 Size: 1205 354 2619 Fragments arranged by size: 2619 1205 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 360 1761 1894 2353 2562 360 Size: 1401 133 459 209 1976 Fragments arranged by size: 1976 1401 459 209 133 TatI w'GTAC_w Cuts at: 927 3693 927 Size: 2766 1412 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3695 3727 3695 Size: 32 4146 Tth111I GACn'n_nGTC Cuts at: 1975 1975 Size: 4178 XbaI T'CTAG_A Cuts at: 1209 1209 Size: 4178 XcmI CCAnnnn_n'nnnnTGG Cuts at: 645 1673 645 Size: 1028 3150 XmaI C'CCGG_G Cuts at: 1198 1198 Size: 4178 ZraI GAC'GTC Cuts at: 127 127 Size: 4178 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI PmlI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI Enzymes that do not cut: AarI AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI BpmI Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PfoI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SrfI SspI StuI SwaI XhoI XmnI