Restriction map for pF1KA0260
 (Circular) (Six-base) MAPSORT of: pF1KA0260.seq  Check: 2797  from: 1  to: 4178



pF1KA0260  4178 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:19  ..

AatII G_ACGT'C

Cuts at:    129     129 
   Size:       4178
 
AccI GT'mk_AC

Cuts at:   1216    1216 
   Size:       4178
 
Acc65I G'GTAC_C

Cuts at:   1194    1194 
   Size:       4178
 
AclI AA'CG_TT

Cuts at:   4018    4018 
   Size:       4178
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    250     575    1127    2003    2435    3182    3450     250 
   Size:        325     552     876     432     747     268     978

  Fragments arranged by size: 

                 978     876     747     552     432     325     268
 
AflIII A'CryG_T

Cuts at:   1530    2634    3611    1530 
   Size:       1104     977    2097

  Fragments arranged by size: 

                2097    1104     977
 
AgeI A'CCGG_T

Cuts at:   2566    2566 
   Size:       4178
 
AlwNI CAG_nnn'CTG

Cuts at:   1483    3050    1483 
   Size:       1567    2611
 
ApaLI G'TGCA_C

Cuts at:   2948    2948 
   Size:       4178
 
ApoI r'AATT_y

Cuts at:   1182    3531    3787    1182 
   Size:       2349     256    1573

  Fragments arranged by size: 

                2349    1573     256
 
AseI AT'TA_AT

Cuts at:     20     991      20 
   Size:        971    3207
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4178
 
AvaI C'yCGr_G

Cuts at:    137    1198     137 
   Size:       1061    3117
 
BamHI G'GATC_C

Cuts at:   1203    1203 
   Size:       4178
 
BanI G'GyrC_C

Cuts at:   1194    1608    1856    1891    1194 
   Size:        414     248      35    3481

  Fragments arranged by size: 

                3481     414     248      35
 
BanII G_rGCy'C

Cuts at:    265    1192    2222     265 
   Size:        927    1030    2221

  Fragments arranged by size: 

                2221    1030     927
 
BbeI G_GCGC'C

Cuts at:   1612    1860    1612 
   Size:        248    3930
 
BbsI GAAGACnn'nnnn_

Cuts at:    580     580 
   Size:       4178
 
BciVI GTATCCnnnnn_n'

Cuts at:   2076    2837    4178    2076 
   Size:        761    1341    2076

  Fragments arranged by size: 

                2076    1341     761
 
BclI T'GATC_A

Cuts at:   1698    1698 
   Size:       4178
 
BglI GCCn_nnn'nGGC

Cuts at:    358     358 
   Size:       4178
 
BglII A'GATC_T

Cuts at:   1693    3730    1693 
   Size:       2037    2141
 
BlpI GC'TnA_GC

Cuts at:   1117    1290    1117 
   Size:        173    4005
 
Bme1580I G_kGCm'C

Cuts at:   1803    1896    2952    1803 
   Size:         93    1056    3029

  Fragments arranged by size: 

                3029    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1390    1471    1806    3950    1390 
   Size:         81     335    2144    1618

  Fragments arranged by size: 

                2144    1618     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    583    2545     583 
   Size:       1962    2216
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    553    1354    2233    2725    3023     553 
   Size:        801     879     492     298    1708

  Fragments arranged by size: 

                1708     879     801     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3882    3882 
   Size:       4178
 
BsaAI yAC'GTr

Cuts at:    720    1037    1531     720 
   Size:        317     494    3367

  Fragments arranged by size: 

                3367     494     317
 
BsaBI GATnn'nnATC

Cuts at:      6    1692    3729       6 
   Size:       1686    2037     455

  Fragments arranged by size: 

                2037    1686     455
 
BsaHI Gr'CG_yC

Cuts at:    126     202    1609    1857    2559    4055     126 
   Size:         76    1407     248     702    1496     249

  Fragments arranged by size: 

                1496    1407     702     249     248      76
 
BsaWI w'CCGG_w

Cuts at:   1367    1888    2566    2840    2987    3398    1367 
   Size:        521     678     274     147     411    2147

  Fragments arranged by size: 

                2147     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    614     644    1157    1187     614 
   Size:         30     513      30    3605

  Fragments arranged by size: 

                3605     513      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    199     202     309     199 
   Size:          3     107    4068

  Fragments arranged by size: 

                4068     107       3
 
BseYI C'CCAG_C

Cuts at:    192     747     854    1072    1603    2361    2938    3434     192 
   Size:        555     107     218     531     758     577     496     936

  Fragments arranged by size: 

                 936     758     577     555     531     496     218     107
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    918     918 
   Size:       4178
 
BsiEI CG_ry'CG

Cuts at:    104     209    1720    1766    2974     104 
   Size:        105    1511      46    1208    1308

  Fragments arranged by size: 

                1511    1308    1208     105      46
 
BsiHKAI G_wGCw'C

Cuts at:    265     299    1192    1970    2160    2952     265 
   Size:         34     893     778     190     792    1491

  Fragments arranged by size: 

                1491     893     792     778     190      34
 
Bsp1286I G_dGCh'C

Cuts at:    265     299    1192    1803    1896    1970    2160    2222    2952 
   Size:         34     893     611      93      74     190      62     730

Cuts at:   2952     265 
   Size:       1491

  Fragments arranged by size: 

                1491     893     730     611     190      93      74      62
                  34
 
BspEI T'CCGG_A

Cuts at:   3398    3398 
   Size:       4178
 
BspHI T'CATG_A

Cuts at:    785    3354     785 
   Size:       2569    1609
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1228    1442    1744    2125    1228 
   Size:        214     302     381    3281

  Fragments arranged by size: 

                3281     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2470    2524    4008    4173      50 
   Size:       2420      54    1484     165      55

  Fragments arranged by size: 

                2420    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    617     806    1067    2090     617 
   Size:        189     261    1023    2705

  Fragments arranged by size: 

                2705    1023     261     189
 
BsrFI r'CCGG_y

Cuts at:    244    2176    2357    2566     244 
   Size:       1932     181     209    1856

  Fragments arranged by size: 

                1932    1856     209     181
 
BssSI C'ACGA_G

Cuts at:   2449    2807    2449 
   Size:        358    3820
 
BstAPI GCAn_nnn'nTGC

Cuts at:    712    1555     712 
   Size:        843    3335
 
BstBI TT'CG_AA

Cuts at:   2539    2539 
   Size:       4178
 
BstYI r'GATC_y

Cuts at:      1    1203    1670    1693    2028    2274    3286    3372    3384 
   Size:       1202     467      23     335     246    1012      86      12

Cuts at:   3384    3730       1 
   Size:        346     449

  Fragments arranged by size: 

                1202    1012     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       4178
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2128    2309    2472    2128 
   Size:        181     163    3834

  Fragments arranged by size: 

                3834     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1175    1421    1175 
   Size:        246    3932
 
DraI TTT'AAA

Cuts at:   1058    1177    1058 
   Size:        119    4059
 
DraIII CAC_nnn'GTG

Cuts at:    328     328 
   Size:       4178
 
DrdI GACnn_nn'nnGTC

Cuts at:   1884    2742    1884 
   Size:        858    3320
 
EaeI y'GGCC_r

Cuts at:    206     239     339     350     803    1763    1937    2328    2355 
   Size:         33     100      11     453     960     174     391      27

Cuts at:   2355    3700     206 
   Size:       1345     684

  Fragments arranged by size: 

                1345     960     684     453     391     174     100      33
                  27      11
 
EagI C'GGCC_G

Cuts at:    206    1763     206 
   Size:       1557    2621
 
EarI CTCTTCn'nnn_

Cuts at:   1103    2201    2411    1103 
   Size:       1098     210    2870

  Fragments arranged by size: 

                2870    1098     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    127    2386    2696    2842    3988     127 
   Size:       2259     310     146    1146     317

  Fragments arranged by size: 

                2259    1146     317     310     146
 
EcoICRI GAG'CTC

Cuts at:    263    1190     263 
   Size:        927    3251
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    250     575    1127    2003    2435    3182    3450     250 
   Size:        325     552     876     432     747     268     978

  Fragments arranged by size: 

                 978     876     747     552     432     325     268
 
EcoO109I rG'GnC_Cy

Cuts at:   1317    1317 
   Size:       4178
 
EcoRI G'AATT_C

Cuts at:   1182    1182 
   Size:       4178
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2197    2197 
   Size:       4178
 
FspI TGC'GCA

Cuts at:   1959    1959 
   Size:       4178
 
HaeII r_GCGC'y

Cuts at:    385    1612    1860    2882    3861     385 
   Size:       1227     248    1022     979     702

  Fragments arranged by size: 

                1227    1022     979     702     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   2020    2050    2052    2082    2020 
   Size:         30       2      30    4116

  Fragments arranged by size: 

                4116      30      30       2
 
HincII GTy'rAC

Cuts at:   1217    1217 
   Size:       4178
 
KasI G'GCGC_C

Cuts at:   1608    1856    1608 
   Size:        248    3930
 
KpnI G_GTAC'C

Cuts at:   1198    1198 
   Size:       4178
 
MluI A'CGCG_T

Cuts at:   3611    3611 
   Size:       4178
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    312    2849    3033     312 
   Size:       2537     184    1457

  Fragments arranged by size: 

                2537    1457     184
 
MscI TGG'CCA

Cuts at:    341     352     805    1939     341 
   Size:         11     453    1134    2580

  Fragments arranged by size: 

                2580    1134     453      11
 
MslI CAynn'nnrTG

Cuts at:   2167    2294    2167 
   Size:        127    4051
 
MspA1I CmG'CkG

Cuts at:   1116    1289    1603    1963    2976    3221    1116 
   Size:        173     314     360    1013     245    2073

  Fragments arranged by size: 

                2073    1013     360     314     245     173
 
NaeI GCC'GGC

Cuts at:    246    2359     246 
   Size:       2113    2065
 
NarI GG'CG_CC

Cuts at:   1609    1857    1609 
   Size:        248    3930
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4178
 
NgoMIV G'CCGG_C

Cuts at:    244    2357     244 
   Size:       2113    2065
 
NspI r_CATG'y

Cuts at:    136    1231    1731    2638     136 
   Size:       1095     500     907    1676

  Fragments arranged by size: 

                1676    1095     907     500
 
PciI A'CATG_T

Cuts at:   2634    2634 
   Size:       4178
 
PflMI CCAn_nnn'nTGG

Cuts at:    348     348 
   Size:       4178
 
PmeI GTTT'AAAC

Cuts at:   1177    1177 
   Size:       4178
 
PmlI CAC'GTG

Cuts at:   1037    1037 
   Size:       4178
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3317    3349    3317 
   Size:         32    4146
 
PstI C_TGCA'G

Cuts at:    881    1225    1910     881 
   Size:        344     685    3149

  Fragments arranged by size: 

                3149     685     344
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4178
 
PvuII CAG'CTG

Cuts at:   1116    1603    1963    1116 
   Size:        487     360    3331

  Fragments arranged by size: 

                3331     487     360
 
RsrII CG'GwC_CG

Cuts at:   2373    2373 
   Size:       4178
 
SacI G_AGCT'C

Cuts at:    265    1192     265 
   Size:        927    3251
 
SalI G'TCGA_C

Cuts at:   1215    1215 
   Size:       4178
 
SapI GCTCTTCn'nnn_

Cuts at:   1103    2201    2411    1103 
   Size:       1098     210    2870

  Fragments arranged by size: 

                2870    1098     210
 
SbfI CC_TGCA'GG

Cuts at:   1225    1225 
   Size:       4178
 
ScaI AGT'ACT

Cuts at:    929    3695     929 
   Size:       2766    1412
 
SfcI C'TryA_G

Cuts at:     33     877    1221    1906    2899    3090      33 
   Size:        844     344     685     993     191    1121

  Fragments arranged by size: 

                1121     993     844     685     344     191
 
SfoI GGC'GCC

Cuts at:   1610    1858    1610 
   Size:        248    3930
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4178
 
SmaI CCC'GGG

Cuts at:   1200    1200 
   Size:       4178
 
SmlI C'TyrA_G

Cuts at:    568    1333    2248    2740    3002     568 
   Size:        765     915     492     262    1744

  Fragments arranged by size: 

                1744     915     765     492     262
 
SnaBI TAC'GTA

Cuts at:    720     720 
   Size:       4178
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4178
 
SphI G_CATG'C

Cuts at:    136    1231    1731     136 
   Size:       1095     500    2583

  Fragments arranged by size: 

                2583    1095     500
 
StyI C'CwwG_G

Cuts at:    107    1312    1666     107 
   Size:       1205     354    2619

  Fragments arranged by size: 

                2619    1205     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    360    1761    1894    2353    2562     360 
   Size:       1401     133     459     209    1976

  Fragments arranged by size: 

                1976    1401     459     209     133
 
TatI w'GTAC_w

Cuts at:    927    3693     927 
   Size:       2766    1412
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3695    3727    3695 
   Size:         32    4146
 
Tth111I GACn'n_nGTC

Cuts at:   1975    1975 
   Size:       4178
 
XbaI T'CTAG_A

Cuts at:   1209    1209 
   Size:       4178
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    645    1673     645 
   Size:       1028    3150
 
XmaI C'CCGG_G

Cuts at:   1198    1198 
   Size:       4178
 
ZraI GAC'GTC

Cuts at:    127     127 
   Size:       4178
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
    BstYI     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PciI    PflMI     PmeI     PmlI     PpiI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI    SnaBI     SpeI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI    BmgBI     BmtI
     BpmI   Bpu10I    BsiWI     BsmI    BsmBI    BsrGI   BssHII   BstEII
    BstXI  BstZ17I   Bsu36I     ClaI    CspCI    EcoNI    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI     PfoI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI     SrfI
     SspI     StuI     SwaI     XhoI     XmnI
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