Restriction map for
pF1KA0267
(Circular) (Six-base) MAPSORT of: pF1KA0267.seq Check: 3476 from: 1 to: 5120
pF1KA0267 5120 bp
With 182 enzymes: SgfI *
February 15, 2010 17:49 ..
AccI GT'mk_AC
Cuts at: 2158 2158
Size: 5120
Acc65I G'GTAC_C
Cuts at: 2136 2136
Size: 5120
AclI AA'CG_TT
Cuts at: 4960 4960
Size: 5120
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 987 1160 1765 2003 2945 3377 4124 4392 987
Size: 173 605 238 942 432 747 268 1715
Fragments arranged by size:
1715 942 747 605 432 268 238 173
AflIII A'CryG_T
Cuts at: 2472 3576 4553 2472
Size: 1104 977 3039
Fragments arranged by size:
3039 1104 977
AgeI A'CCGG_T
Cuts at: 3508 3508
Size: 5120
AleI CACnn'nnGTG
Cuts at: 1658 1658
Size: 5120
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 196 196
Size: 5120
AlwNI CAG_nnn'CTG
Cuts at: 796 808 2425 3992 796
Size: 12 1617 1567 1924
Fragments arranged by size:
1924 1617 1567 12
ApaI G_GGCC'C
Cuts at: 396 396
Size: 5120
ApaLI G'TGCA_C
Cuts at: 3890 3890
Size: 5120
ApoI r'AATT_y
Cuts at: 1278 1446 2082 2124 4473 4729 1278
Size: 168 636 42 2349 256 1669
Fragments arranged by size:
2349 1669 636 256 168 42
AseI AT'TA_AT
Cuts at: 20 20
Size: 5120
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5120
AvaI C'yCGr_G
Cuts at: 164 2140 164
Size: 1976 3144
BamHI G'GATC_C
Cuts at: 2145 2145
Size: 5120
BanI G'GyrC_C
Cuts at: 132 778 1594 1817 2136 2550 2798 2833 132
Size: 646 816 223 319 414 248 35 2419
Fragments arranged by size:
2419 816 646 414 319 248 223 35
BanII G_rGCy'C
Cuts at: 171 396 2134 3164 171
Size: 225 1738 1030 2127
Fragments arranged by size:
2127 1738 1030 225
BbeI G_GCGC'C
Cuts at: 2554 2802 2554
Size: 248 4872
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 215 249 215
Size: 34 5086
BciVI GTATCCnnnnn_n'
Cuts at: 3018 3779 5120 3018
Size: 761 1341 3018
Fragments arranged by size:
3018 1341 761
BclI T'GATC_A
Cuts at: 1731 2640 1731
Size: 909 4211
BglII A'GATC_T
Cuts at: 2635 4672 2635
Size: 2037 3083
BlpI GC'TnA_GC
Cuts at: 2232 2232
Size: 5120
Bme1580I G_kGCm'C
Cuts at: 135 396 2745 2838 3894 135
Size: 261 2349 93 1056 1361
Fragments arranged by size:
2349 1361 1056 261 93
BmrI ACTGGGnnnn_n'
Cuts at: 231 2332 2413 2748 4892 231
Size: 2101 81 335 2144 459
Fragments arranged by size:
2144 2101 459 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3487 3487
Size: 5120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 956 1055 1140 956
Size: 99 85 4936
Fragments arranged by size:
4936 99 85
Bpu10I CC'TnA_GC
Cuts at: 499 499
Size: 5120
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2296 3175 3667 3965 2296
Size: 879 492 298 3451
Fragments arranged by size:
3451 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4824 4824
Size: 5120
BsaAI yAC'GTr
Cuts at: 1205 2473 1205
Size: 1268 3852
BsaBI GATnn'nnATC
Cuts at: 6 1874 2634 4671 6
Size: 1868 760 2037 455
Fragments arranged by size:
2037 1868 760 455
BsaHI Gr'CG_yC
Cuts at: 149 212 2551 2799 3501 4997 149
Size: 63 2339 248 702 1496 272
Fragments arranged by size:
2339 1496 702 272 248 63
BsaWI w'CCGG_w
Cuts at: 1712 2309 2830 3508 3782 3929 4340 1712
Size: 597 521 678 274 147 411 2492
Fragments arranged by size:
2492 678 597 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 265 295 1069 1099 1346 1376 265
Size: 30 774 30 247 30 4009
Fragments arranged by size:
4009 774 247 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 190 286 313 900 190
Size: 96 27 587 4410
Fragments arranged by size:
4410 587 96 27
BseYI C'CCAG_C
Cuts at: 2545 3303 3880 4376 2545
Size: 758 577 496 3289
Fragments arranged by size:
3289 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 532 388
Size: 144 4976
BsiEI CG_ry'CG
Cuts at: 104 2662 2708 3916 104
Size: 2558 46 1208 1308
Fragments arranged by size:
2558 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2134 2912 3102 3894 2134
Size: 778 190 792 3360
Fragments arranged by size:
3360 792 778 190
BsmI GAATG_Cn'
Cuts at: 464 464
Size: 5120
Bsp1286I G_dGCh'C
Cuts at: 135 171 396 2134 2745 2838 2912 3102 3164
Size: 36 225 1738 611 93 74 190 62
Cuts at: 3164 3894 135
Size: 730 1361
Fragments arranged by size:
1738 1361 730 611 225 190 93 74
62 36
BspEI T'CCGG_A
Cuts at: 1712 4340 1712
Size: 2628 2492
BspHI T'CATG_A
Cuts at: 4296 4296
Size: 5120
BspMI ACCTGCnnnn'nnnn_
Cuts at: 335 586 2170 2384 2686 3067 335
Size: 251 1584 214 302 381 2388
Fragments arranged by size:
2388 1584 381 302 251 214
BsrBI CCG'CTC
Cuts at: 50 3412 3466 4950 5115 50
Size: 3362 54 1484 165 55
Fragments arranged by size:
3362 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1025 1493 1610 3032 1025
Size: 468 117 1422 3113
Fragments arranged by size:
3113 1422 468 117
BsrFI r'CCGG_y
Cuts at: 308 412 1938 3118 3299 3508 308
Size: 104 1526 1180 181 209 1920
Fragments arranged by size:
1920 1526 1180 209 181 104
BssSI C'ACGA_G
Cuts at: 826 3391 3749 826
Size: 2565 358 2197
Fragments arranged by size:
2565 2197 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1024 1363 1699 2497 1024
Size: 339 336 798 3647
Fragments arranged by size:
3647 798 339 336
BstBI TT'CG_AA
Cuts at: 627 3481 627
Size: 2854 2266
BstXI CCAn_nnnn'nTGG
Cuts at: 934 934
Size: 5120
BstYI r'GATC_y
Cuts at: 1 986 1009 2145 2612 2635 2970 3216 4228
Size: 985 23 1136 467 23 335 246 1012
Cuts at: 4228 4314 4326 4672 1
Size: 86 12 346 449
Fragments arranged by size:
1136 1012 985 467 449 346 335 246
86 23 23 12
BtgI C'CryG_G
Cuts at: 107 144 263 1220 107
Size: 37 119 957 4007
Fragments arranged by size:
4007 957 119 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 982 1794 3070 3251 3414 982
Size: 812 1276 181 163 2688
Fragments arranged by size:
2688 1276 812 181 163
BtsI GCAGTG_nn'
Cuts at: 212 1598 2363 212
Size: 1386 765 2969
Fragments arranged by size:
2969 1386 765
DraI TTT'AAA
Cuts at: 2119 2119
Size: 5120
DrdI GACnn_nn'nnGTC
Cuts at: 2826 3684 2826
Size: 858 4262
EaeI y'GGCC_r
Cuts at: 1502 1940 2705 2879 3270 3297 4642 1502
Size: 438 765 174 391 27 1345 1980
Fragments arranged by size:
1980 1345 765 438 391 174 27
EagI C'GGCC_G
Cuts at: 2705 2705
Size: 5120
EarI CTCTTCn'nnn_
Cuts at: 382 1374 3143 3353 382
Size: 992 1769 210 2149
Fragments arranged by size:
2149 1769 992 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 130 263 3328 3638 3784 4930 130
Size: 133 3065 310 146 1146 320
Fragments arranged by size:
3065 1146 320 310 146 133
EcoICRI GAG'CTC
Cuts at: 2132 2132
Size: 5120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 956 987 1055 1140 1160 1765 2003 2945 3377
Size: 31 68 85 20 605 238 942 432
Cuts at: 3377 4124 4392 956
Size: 747 268 1684
Fragments arranged by size:
1684 942 747 605 432 268 238 85
68 31 20
EcoO109I rG'GnC_Cy
Cuts at: 2259 2259
Size: 5120
EcoRI G'AATT_C
Cuts at: 2124 2124
Size: 5120
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3139 3139
Size: 5120
FspI TGC'GCA
Cuts at: 2901 2901
Size: 5120
HaeII r_GCGC'y
Cuts at: 323 1955 2554 2802 3824 4803 323
Size: 1632 599 248 1022 979 640
Fragments arranged by size:
1632 1022 979 640 599 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 265 297 417 449 2091 2123 2962 2992 2994
Size: 32 120 32 1642 32 839 30 2
Cuts at: 2994 3024 265
Size: 30 2361
Fragments arranged by size:
2361 1642 839 120 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2159 2159
Size: 5120
KasI G'GCGC_C
Cuts at: 2550 2798 2550
Size: 248 4872
KpnI G_GTAC'C
Cuts at: 2140 2140
Size: 5120
MluI A'CGCG_T
Cuts at: 4553 4553
Size: 5120
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 547 1070 3791 3975 547
Size: 523 2721 184 1692
Fragments arranged by size:
2721 1692 523 184
MscI TGG'CCA
Cuts at: 1504 2881 1504
Size: 1377 3743
MslI CAynn'nnrTG
Cuts at: 967 1462 1603 1658 3109 3236 967
Size: 495 141 55 1451 127 2851
Fragments arranged by size:
2851 1451 495 141 127 55
MspA1I CmG'CkG
Cuts at: 934 1766 1776 2231 2545 2905 3918 4163 934
Size: 832 10 455 314 360 1013 245 1891
Fragments arranged by size:
1891 1013 832 455 360 314 245 10
NaeI GCC'GGC
Cuts at: 3301 3301
Size: 5120
NarI GG'CG_CC
Cuts at: 2551 2799 2551
Size: 248 4872
NcoI C'CATG_G
Cuts at: 107 263 107
Size: 156 4964
NdeI CA'TA_TG
Cuts at: 1911 1911
Size: 5120
NgoMIV G'CCGG_C
Cuts at: 3299 3299
Size: 5120
NspI r_CATG'y
Cuts at: 2173 2673 3580 2173
Size: 500 907 3713
Fragments arranged by size:
3713 907 500
PciI A'CATG_T
Cuts at: 3576 3576
Size: 5120
PflMI CCAn_nnn'nTGG
Cuts at: 1089 2025 1089
Size: 936 4184
PfoI T'CCnGG_A
Cuts at: 1117 1117
Size: 5120
PmeI GTTT'AAAC
Cuts at: 2119 2119
Size: 5120
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4259 4291 4259
Size: 32 5088
PshAI GACnn'nnGTC
Cuts at: 944 944
Size: 5120
PspOMI G'GGCC_C
Cuts at: 392 392
Size: 5120
PstI C_TGCA'G
Cuts at: 2167 2852 2167
Size: 685 4435
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5120
PvuII CAG'CTG
Cuts at: 934 2545 2905 934
Size: 1611 360 3149
Fragments arranged by size:
3149 1611 360
RsrII CG'GwC_CG
Cuts at: 3315 3315
Size: 5120
SacI G_AGCT'C
Cuts at: 2134 2134
Size: 5120
SalI G'TCGA_C
Cuts at: 2157 2157
Size: 5120
SapI GCTCTTCn'nnn_
Cuts at: 382 1374 3143 3353 382
Size: 992 1769 210 2149
Fragments arranged by size:
2149 1769 992 210
SbfI CC_TGCA'GG
Cuts at: 2167 2167
Size: 5120
ScaI AGT'ACT
Cuts at: 4637 4637
Size: 5120
SfcI C'TryA_G
Cuts at: 33 2163 2848 3841 4032 33
Size: 2130 685 993 191 1121
Fragments arranged by size:
2130 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2552 2800 2552
Size: 248 4872
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5120
SmaI CCC'GGG
Cuts at: 2142 2142
Size: 5120
SmlI C'TyrA_G
Cuts at: 2275 3190 3682 3944 2275
Size: 915 492 262 3451
Fragments arranged by size:
3451 915 492 262
SnaBI TAC'GTA
Cuts at: 1205 1205
Size: 5120
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5120
SphI G_CATG'C
Cuts at: 2173 2673 2173
Size: 500 4620
SspI AAT'ATT
Cuts at: 1392 1392
Size: 5120
StuI AGG'CCT
Cuts at: 1100 1100
Size: 5120
StyI C'CwwG_G
Cuts at: 107 263 993 1499 1957 1991 2254 2608 107
Size: 156 730 506 458 34 263 354 2619
Fragments arranged by size:
2619 730 506 458 354 263 156 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1012 1648 2703 2836 3295 3504 1012
Size: 636 1055 133 459 209 2628
Fragments arranged by size:
2628 1055 636 459 209 133
TatI w'GTAC_w
Cuts at: 4635 4635
Size: 5120
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4637 4669 4637
Size: 32 5088
Tth111I GACn'n_nGTC
Cuts at: 2105 2917 2105
Size: 812 4308
XbaI T'CTAG_A
Cuts at: 2151 2151
Size: 5120
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2615 2615
Size: 5120
XmaI C'CCGG_G
Cuts at: 2140 2140
Size: 5120
XmnI GAAnn'nnTTC
Cuts at: 1977 1977
Size: 5120
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI BsiWI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NheI NotI NruI NsiI PacI PasI PmlI PpuMI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SrfI SwaI XhoI ZraI