Restriction map for
pF1KA0284
(Circular) (Six-base) MAPSORT of: pF1KA0284.seq Check: 2703 from: 1 to: 7670
pF1KA0284 7670 bp
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 246 649 1035 246
Size: 403 386 6881
Fragments arranged by size:
6881 403 386
AccI GT'mk_AC
Cuts at: 4708 4708
Size: 7670
Acc65I G'GTAC_C
Cuts at: 4686 4686
Size: 7670
AclI AA'CG_TT
Cuts at: 7510 7510
Size: 7670
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 964 3284 3582 4372 4405 5495 5927 6674
Size: 804 2320 298 790 33 1090 432 747
Cuts at: 6674 6942 160
Size: 268 888
Fragments arranged by size:
2320 1090 888 804 790 747 432 298
268 33
AfeI AGC'GCT
Cuts at: 1150 1150
Size: 7670
AflIII A'CryG_T
Cuts at: 179 3668 5022 6126 7103 179
Size: 3489 1354 1104 977 746
Fragments arranged by size:
3489 1354 1104 977 746
AgeI A'CCGG_T
Cuts at: 6058 6058
Size: 7670
AhdI GACnn_n'nnGTC
Cuts at: 2745 2745
Size: 7670
AleI CACnn'nnGTG
Cuts at: 1636 1636
Size: 7670
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1814 1814
Size: 7670
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4201 4233 4201
Size: 32 7638
AlwNI CAG_nnn'CTG
Cuts at: 1419 2580 2832 3307 3370 3466 3894 4975 6542
Size: 1161 252 475 63 96 428 1081 1567
Cuts at: 6542 1419
Size: 2547
Fragments arranged by size:
2547 1567 1161 1081 475 428 252 96
63
ApaI G_GGCC'C
Cuts at: 1516 2041 2228 2262 2810 3285 3609 3792 1516
Size: 525 187 34 548 475 324 183 5394
Fragments arranged by size:
5394 548 525 475 324 187 183 34
ApaLI G'TGCA_C
Cuts at: 6440 6440
Size: 7670
ApoI r'AATT_y
Cuts at: 4584 4674 7023 7279 4584
Size: 90 2349 256 4975
Fragments arranged by size:
4975 2349 256 90
AseI AT'TA_AT
Cuts at: 20 20
Size: 7670
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7670
AvaI C'yCGr_G
Cuts at: 616 789 864 1272 1453 1920 2173 2263 2591
Size: 173 75 408 181 467 253 90 328
Cuts at: 2591 2724 2880 3008 3170 3454 3612 3753 3785
Size: 133 156 128 162 284 158 141 32
Cuts at: 3785 3800 4031 4057 4106 4690 616
Size: 15 231 26 49 584 3596
Fragments arranged by size:
3596 584 467 408 328 284 253 231
181 173 162 158 156 141 133 128
90 75 49 32 26 15
AvrII C'CTAG_G
Cuts at: 2361 2361
Size: 7670
BamHI G'GATC_C
Cuts at: 2124 4695 2124
Size: 2571 5099
BanI G'GyrC_C
Cuts at: 276 621 1020 1379 1851 2887 3644 4083 4341
Size: 345 399 359 472 1036 757 439 258
Cuts at: 4341 4686 5100 5348 5383 276
Size: 345 414 248 35 2563
Fragments arranged by size:
2563 1036 757 472 439 414 399 359
345 345 258 248 35
BanII G_rGCy'C
Cuts at: 467 512 566 1432 1516 1861 2041 2215 2228
Size: 45 54 866 84 345 180 174 13
Cuts at: 2228 2262 2810 3285 3353 3609 3661 3780 3792
Size: 34 548 475 68 256 52 119 12
Cuts at: 3792 3964 4534 4684 5714 467
Size: 172 570 150 1030 2423
Fragments arranged by size:
2423 1030 866 570 548 475 345 256
180 174 172 150 119 84 68 54
52 45 34 13 12
BbeI G_GCGC'C
Cuts at: 280 1383 3648 5104 5352 280
Size: 1103 2265 1456 248 2598
Fragments arranged by size:
2598 2265 1456 1103 248
BbsI GAAGACnn'nnnn_
Cuts at: 1315 4360 1315
Size: 3045 4625
BbvCI CC'TCA_GC
Cuts at: 2003 2137 3274 3991 4548 2003
Size: 134 1137 717 557 5125
Fragments arranged by size:
5125 1137 717 557 134
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 855 889 2455 2489 90
Size: 34 731 34 1566 34 5271
Fragments arranged by size:
5271 1566 731 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5568 6329 7670 5568
Size: 761 1341 5568
Fragments arranged by size:
5568 1341 761
BclI T'GATC_A
Cuts at: 5190 5190
Size: 7670
BglI GCCn_nnn'nGGC
Cuts at: 1003 1511 1828 1900 2036 2108 2662 2671 2976
Size: 508 317 72 136 72 554 9 305
Cuts at: 2976 3112 1003
Size: 136 5561
Fragments arranged by size:
5561 554 508 317 305 136 136 72
72 9
BglII A'GATC_T
Cuts at: 5185 7222 5185
Size: 2037 5633
BlpI GC'TnA_GC
Cuts at: 533 2982 3192 3371 3883 4782 533
Size: 2449 210 179 512 899 3421
Fragments arranged by size:
3421 2449 899 512 210 179
Bme1580I G_kGCm'C
Cuts at: 1025 1516 2041 2228 2262 2810 2841 2907 3285
Size: 491 525 187 34 548 31 66 378
Cuts at: 3285 3609 3624 3792 4346 5295 5388 6444 1025
Size: 324 15 168 554 949 93 1056 2251
Fragments arranged by size:
2251 1056 949 554 548 525 491 378
324 187 168 93 66 34 31 15
BmgBI CAC'GTC
Cuts at: 2763 3671 3906 2763
Size: 908 235 6527
Fragments arranged by size:
6527 908 235
BmrI ACTGGGnnnn_n'
Cuts at: 1149 1229 1243 1407 1720 1822 2006 3023 3967
Size: 80 14 164 313 102 184 1017 944
Cuts at: 3967 4436 4882 4963 5298 7442 1149
Size: 469 446 81 335 2144 1377
Fragments arranged by size:
2144 1377 1017 944 469 446 335 313
184 164 102 81 80 14
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 393 1818 2934 3711 6037 393
Size: 1425 1116 777 2326 2026
Fragments arranged by size:
2326 2026 1425 1116 777
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 1683 2327 2541 2689 3534 3753 211
Size: 1472 644 214 148 845 219 4128
Fragments arranged by size:
4128 1472 845 644 219 214 148
Bpu10I CC'TnA_GC
Cuts at: 2003 2137 3274 3347 3991 4548 2003
Size: 134 1137 73 644 557 5125
Fragments arranged by size:
5125 1137 644 557 134 73
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 1282 4846 5725 6217 6515 211
Size: 1071 3564 879 492 298 1366
Fragments arranged by size:
3564 1366 1071 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 351 586 823 1609 7374 351
Size: 235 237 786 5765 647
Fragments arranged by size:
5765 786 647 237 235
BsaAI yAC'GTr
Cuts at: 2231 5023 2231
Size: 2792 4878
BsaBI GATnn'nnATC
Cuts at: 6 742 4273 5184 7221 6
Size: 736 3531 911 2037 455
Fragments arranged by size:
3531 2037 911 736 455
BsaHI Gr'CG_yC
Cuts at: 277 446 1380 2402 2741 3645 4145 5101 5349
Size: 169 934 1022 339 904 500 956 248
Cuts at: 5349 6051 7547 277
Size: 702 1496 400
Fragments arranged by size:
1496 1022 956 934 904 702 500 400
339 248 169
BsaWI w'CCGG_w
Cuts at: 353 1467 2073 4859 5380 6058 6332 6479 6890
Size: 1114 606 2786 521 678 274 147 411
Cuts at: 6890 353
Size: 1133
Fragments arranged by size:
2786 1133 1114 678 606 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2226 2256 2599 2629 2957 2987 3709 3739 3970
Size: 30 343 30 328 30 722 30 231
Cuts at: 3970 4000 2226
Size: 30 5896
Fragments arranged by size:
5896 722 343 328 231 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 232 300 1588 1657 2070 2131 2671 3049 3190
Size: 68 1288 69 413 61 540 378 141
Cuts at: 3190 3697 3700 3835 3838 3954 4051 4195 232
Size: 507 3 135 3 116 97 144 3707
Fragments arranged by size:
3707 1288 540 507 413 378 144 141
135 116 97 69 68 61 3 3
BseYI C'CCAG_C
Cuts at: 449 565 1015 1242 1323 1447 1547 1772 1889
Size: 116 450 227 81 124 100 225 117
Cuts at: 1889 2095 2375 2380 2388 2478 2529 2809 2825
Size: 206 280 5 8 90 51 280 16
Cuts at: 2825 3064 3162 3288 3940 4003 4220 4503 4615
Size: 239 98 126 652 63 217 283 112
Cuts at: 4615 5095 5853 6430 6926 449
Size: 480 758 577 496 1193
Fragments arranged by size:
1193 758 652 577 496 480 450 283
280 280 239 227 225 217 206 126
124 117 116 112 100 98 90 81
63 51 16 8 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 514 706 859 874 2855 3871 4066 4423
Size: 294 192 153 15 1981 1016 195 357
Cuts at: 4423 4475 220
Size: 52 3415
Fragments arranged by size:
3415 1981 1016 357 294 195 192 153
52 15
BsiEI CG_ry'CG
Cuts at: 104 1013 1220 1439 5212 5258 6466 104
Size: 909 207 219 3773 46 1208 1308
Fragments arranged by size:
3773 1308 1208 909 219 207 46
BsiHKAI G_wGCw'C
Cuts at: 512 1432 1838 4256 4684 5462 5652 6444 512
Size: 920 406 2418 428 778 190 792 1738
Fragments arranged by size:
2418 1738 920 792 778 428 406 190
BsmBI CGTCTCn'nnnn_
Cuts at: 2638 2644 2638
Size: 6 7664
Bsp1286I G_dGCh'C
Cuts at: 467 512 566 1025 1432 1516 1838 1861 2041
Size: 45 54 459 407 84 322 23 180
Cuts at: 2041 2215 2228 2262 2810 2841 2907 3285 3353
Size: 174 13 34 548 31 66 378 68
Cuts at: 3353 3609 3624 3661 3780 3792 3964 4256 4346
Size: 256 15 37 119 12 172 292 90
Cuts at: 4346 4534 4684 5295 5388 5462 5652 5714 6444
Size: 188 150 611 93 74 190 62 730
Cuts at: 6444 467
Size: 1693
Fragments arranged by size:
1693 730 611 548 459 407 378 322
292 256 190 188 180 174 172 150
119 93 90 84 74 68 66 62
54 45 37 34 31 23 15 13
12
BspEI T'CCGG_A
Cuts at: 6890 6890
Size: 7670
BspHI T'CATG_A
Cuts at: 6846 6846
Size: 7670
BspMI ACCTGCnnnn'nnnn_
Cuts at: 246 649 929 1035 4466 4720 4934 5236 5617
Size: 403 280 106 3431 254 214 302 381
Cuts at: 5617 246
Size: 2299
Fragments arranged by size:
3431 2299 403 381 302 280 254 214
106
BsrBI CCG'CTC
Cuts at: 50 673 1385 1480 2804 3230 3327 3611 5962
Size: 623 712 95 1324 426 97 284 2351
Cuts at: 5962 6016 7500 7665 50
Size: 54 1484 165 55
Fragments arranged by size:
2351 1484 1324 712 623 426 284 165
97 95 55 54
BsrDI GCAATG_nn'
Cuts at: 5582 5582
Size: 7670
BsrFI r'CCGG_y
Cuts at: 624 1215 1288 2970 3359 3427 3647 5668 5849
Size: 591 73 1682 389 68 220 2021 181
Cuts at: 5849 6058 624
Size: 209 2236
Fragments arranged by size:
2236 2021 1682 591 389 220 209 181
73 68
BssSI C'ACGA_G
Cuts at: 631 958 3639 5941 6299 631
Size: 327 2681 2302 358 2002
Fragments arranged by size:
2681 2302 2002 358 327
BstAPI GCAn_nnn'nTGC
Cuts at: 3466 3865 5047 3466
Size: 399 1182 6089
Fragments arranged by size:
6089 1182 399
BstBI TT'CG_AA
Cuts at: 6031 6031
Size: 7670
BstXI CCAn_nnnn'nTGG
Cuts at: 1509 1672 1509
Size: 163 7507
BstYI r'GATC_y
Cuts at: 1 578 2124 3926 4274 4695 5162 5185 5520
Size: 577 1546 1802 348 421 467 23 335
Cuts at: 5520 5766 6778 6864 6876 7222 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1802 1546 1012 577 467 449 421 348
346 335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 539 3101 4579 539
Size: 2562 1478 3630
Fragments arranged by size:
3630 2562 1478
BtgI C'CryG_G
Cuts at: 513 1203 1494 1508 1538 2961 3353 3527 3578
Size: 690 291 14 30 1423 392 174 51
Cuts at: 3578 3702 513
Size: 124 4481
Fragments arranged by size:
4481 1423 690 392 291 174 124 51
30 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2156 5620 5801 5964 2156
Size: 3464 181 163 3862
Fragments arranged by size:
3862 3464 181 163
BtsI GCAGTG_nn'
Cuts at: 295 3498 3624 4913 295
Size: 3203 126 1289 3052
Fragments arranged by size:
3203 3052 1289 126
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1185 1220 1185
Size: 35 7635
DraI TTT'AAA
Cuts at: 4669 4669
Size: 7670
DraIII CAC_nnn'GTG
Cuts at: 1816 3936 1816
Size: 2120 5550
DrdI GACnn_nn'nnGTC
Cuts at: 925 5376 6234 925
Size: 4451 858 2361
Fragments arranged by size:
4451 2361 858
EaeI y'GGCC_r
Cuts at: 839 995 1010 1217 1436 1712 1807 2109 2582
Size: 156 15 207 219 276 95 302 473
Cuts at: 2582 2997 3361 3407 3486 3530 3908 4151 4481
Size: 415 364 46 79 44 378 243 330
Cuts at: 4481 5255 5429 5820 5847 7192 839
Size: 774 174 391 27 1345 1317
Fragments arranged by size:
1345 1317 774 473 415 391 378 364
330 302 276 243 219 207 174 156
95 79 46 44 27 15
EagI C'GGCC_G
Cuts at: 1010 1217 1436 5255 1010
Size: 207 219 3819 3425
Fragments arranged by size:
3819 3425 219 207
EarI CTCTTCn'nnn_
Cuts at: 2110 5693 5903 2110
Size: 3583 210 3877
Fragments arranged by size:
3877 3583 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 683 1846 5878 6188 6334 7480 683
Size: 1163 4032 310 146 1146 873
Fragments arranged by size:
4032 1163 1146 873 310 146
EcoICRI GAG'CTC
Cuts at: 510 1430 4682 510
Size: 920 3252 3498
Fragments arranged by size:
3498 3252 920
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 211 964 1683 2327 2541 2689 3284 3534
Size: 51 753 719 644 214 148 595 250
Cuts at: 3534 3582 3753 4372 4405 5495 5927 6674 6942
Size: 48 171 619 33 1090 432 747 268
Cuts at: 6942 160
Size: 888
Fragments arranged by size:
1090 888 753 747 719 644 619 595
432 268 250 214 171 148 51 48
33
EcoNI CCTnn'n_nnAGG
Cuts at: 997 1396 1930 2922 3600 3919 997
Size: 399 534 992 678 319 4748
Fragments arranged by size:
4748 992 678 534 399 319
EcoO109I rG'GnC_Cy
Cuts at: 487 987 1074 1182 1513 1842 1970 2170 2224
Size: 500 87 108 331 329 128 200 54
Cuts at: 2224 2258 2259 2358 2577 2877 3005 3014 3332
Size: 34 1 99 219 300 128 9 318
Cuts at: 3332 3583 3605 3789 4308 4557 4809 487
Size: 251 22 184 519 249 252 3348
Fragments arranged by size:
3348 519 500 331 329 318 300 252
251 249 219 200 184 128 128 108
99 87 54 34 22 9 1
EcoRI G'AATT_C
Cuts at: 4674 4674
Size: 7670
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4296 5689 4296
Size: 1393 6277
FseI GG_CCGG'CC
Cuts at: 3363 3363
Size: 7670
FspI TGC'GCA
Cuts at: 112 1984 5451 112
Size: 1872 3467 2331
Fragments arranged by size:
3467 2331 1872
FspAI rTGC'GCAy
Cuts at: 112 1984 112
Size: 1872 5798
HaeII r_GCGC'y
Cuts at: 280 456 480 1152 1203 1383 1786 2250 2536
Size: 176 24 672 51 180 403 464 286
Cuts at: 2536 2982 3648 3986 5104 5352 6374 7353 280
Size: 446 666 338 1118 248 1022 979 597
Fragments arranged by size:
1118 1022 979 672 666 597 464 446
403 338 286 248 180 176 51 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 805 837 1897 1929 2226 2258 2599 2631 2731
Size: 32 1060 32 297 32 341 32 100
Cuts at: 2731 2763 2916 2948 3316 3348 3655 3687 4152
Size: 32 153 32 368 32 307 32 465
Cuts at: 4152 4184 5512 5542 5544 5574 805
Size: 32 1328 30 2 30 2901
Fragments arranged by size:
2901 1328 1060 465 368 341 307 297
153 100 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 4709 4709
Size: 7670
KasI G'GCGC_C
Cuts at: 276 1379 3644 5100 5348 276
Size: 1103 2265 1456 248 2598
Fragments arranged by size:
2598 2265 1456 1103 248
KpnI G_GTAC'C
Cuts at: 4690 4690
Size: 7670
MluI A'CGCG_T
Cuts at: 7103 7103
Size: 7670
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 200 1661 2102 2135 3110 3257 3659 6341 6525
Size: 1461 441 33 975 147 402 2682 184
Cuts at: 6525 200
Size: 1345
Fragments arranged by size:
2682 1461 1345 975 441 402 184 147
33
MscI TGG'CCA
Cuts at: 997 2999 3409 3910 4153 4483 5431 997
Size: 2002 410 501 243 330 948 3236
Fragments arranged by size:
3236 2002 948 501 410 330 243
MslI CAynn'nnrTG
Cuts at: 184 1636 5659 5786 184
Size: 1452 4023 127 2068
Fragments arranged by size:
4023 2068 1452 127
MspA1I CmG'CkG
Cuts at: 252 780 1015 1037 1551 1776 1889 2007 2869
Size: 528 235 22 514 225 113 118 862
Cuts at: 2869 3145 3166 3367 4136 4224 4413 4596 4604
Size: 276 21 201 769 88 189 183 8
Cuts at: 4604 4781 5095 5455 6468 6713 252
Size: 177 314 360 1013 245 1209
Fragments arranged by size:
1209 1013 862 769 528 514 360 314
276 245 235 225 201 189 183 177
118 113 88 22 21 8
NaeI GCC'GGC
Cuts at: 626 1217 1290 3361 3429 3649 5851 626
Size: 591 73 2071 68 220 2202 2445
Fragments arranged by size:
2445 2202 2071 591 220 73 68
NarI GG'CG_CC
Cuts at: 277 1380 3645 5101 5349 277
Size: 1103 2265 1456 248 2598
Fragments arranged by size:
2598 2265 1456 1103 248
NcoI C'CATG_G
Cuts at: 1494 3527 1494
Size: 2033 5637
NgoMIV G'CCGG_C
Cuts at: 624 1215 1288 3359 3427 3647 5849 624
Size: 591 73 2071 68 220 2202 2445
Fragments arranged by size:
2445 2202 2071 591 220 73 68
NspI r_CATG'y
Cuts at: 183 1989 4002 4723 5223 6130 183
Size: 1806 2013 721 500 907 1723
Fragments arranged by size:
2013 1806 1723 907 721 500
PasI CC'CwG_GG
Cuts at: 889 1864 3336 889
Size: 975 1472 5223
Fragments arranged by size:
5223 1472 975
PciI A'CATG_T
Cuts at: 179 6126 179
Size: 5947 1723
PflMI CCAn_nnn'nTGG
Cuts at: 1779 2350 2521 3894 3936 1779
Size: 571 171 1373 42 5513
Fragments arranged by size:
5513 1373 571 171 42
PfoI T'CCnGG_A
Cuts at: 116 212 3550 4622 116
Size: 96 3338 1072 3164
Fragments arranged by size:
3338 3164 1072 96
PmeI GTTT'AAAC
Cuts at: 4669 4669
Size: 7670
PmlI CAC'GTG
Cuts at: 2231 2231
Size: 7670
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6809 6841 6809
Size: 32 7638
PpuMI rG'GwC_Cy
Cuts at: 487 987 1074 1182 1970 2577 2877 3014 3332
Size: 500 87 108 788 607 300 137 318
Cuts at: 3332 3583 4308 487
Size: 251 725 3849
Fragments arranged by size:
3849 788 725 607 500 318 300 251
137 108 87
PspOMI G'GGCC_C
Cuts at: 1512 2037 2224 2258 2806 3281 3605 3788 1512
Size: 525 187 34 548 475 324 183 5394
Fragments arranged by size:
5394 548 525 475 324 187 183 34
PstI C_TGCA'G
Cuts at: 257 727 1058 1793 4717 5402 257
Size: 470 331 735 2924 685 2525
Fragments arranged by size:
2924 2525 735 685 470 331
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7670
PvuII CAG'CTG
Cuts at: 252 1551 2007 2869 3166 3367 4136 4224 4413
Size: 1299 456 862 297 201 769 88 189
Cuts at: 4413 4604 5095 5455 252
Size: 191 491 360 2467
Fragments arranged by size:
2467 1299 862 769 491 456 360 297
201 191 189 88
RsrII CG'GwC_CG
Cuts at: 5865 5865
Size: 7670
SacI G_AGCT'C
Cuts at: 512 1432 4684 512
Size: 920 3252 3498
Fragments arranged by size:
3498 3252 920
SalI G'TCGA_C
Cuts at: 4707 4707
Size: 7670
SanDI GG'GwC_CC
Cuts at: 487 1074 1182 1970 3014 3332 3583 487
Size: 587 108 788 1044 318 251 4574
Fragments arranged by size:
4574 1044 788 587 318 251 108
SapI GCTCTTCn'nnn_
Cuts at: 5693 5903 5693
Size: 210 7460
SbfI CC_TGCA'GG
Cuts at: 4717 4717
Size: 7670
ScaI AGT'ACT
Cuts at: 7187 7187
Size: 7670
SexAI A'CCwGG_T
Cuts at: 1664 1664
Size: 7670
SfcI C'TryA_G
Cuts at: 33 253 723 1054 1789 2572 4713 5398 6391
Size: 220 470 331 735 783 2141 685 993
Cuts at: 6391 6582 33
Size: 191 1121
Fragments arranged by size:
2141 1121 993 783 735 685 470 331
220 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1003 2662 2671 1003
Size: 1659 9 6002
Fragments arranged by size:
6002 1659 9
SfoI GGC'GCC
Cuts at: 278 1381 3646 5102 5350 278
Size: 1103 2265 1456 248 2598
Fragments arranged by size:
2598 2265 1456 1103 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7670
SmaI CCC'GGG
Cuts at: 791 1922 2265 2593 2882 3172 3787 3802 4033
Size: 1131 343 328 289 290 615 15 231
Cuts at: 4033 4692 791
Size: 659 3769
Fragments arranged by size:
3769 1131 659 615 343 328 290 289
231 15
SmlI C'TyrA_G
Cuts at: 226 1297 4825 5740 6232 6494 226
Size: 1071 3528 915 492 262 1402
Fragments arranged by size:
3528 1402 1071 915 492 262
SpeI A'CTAG_T
Cuts at: 67 3445 67
Size: 3378 4292
SphI G_CATG'C
Cuts at: 1989 4723 5223 1989
Size: 2734 500 4436
Fragments arranged by size:
4436 2734 500
StuI AGG'CCT
Cuts at: 325 1105 3220 325
Size: 780 2115 4775
Fragments arranged by size:
4775 2115 780
StyI C'CwwG_G
Cuts at: 482 833 998 1025 1187 1494 1556 2254 2361
Size: 351 165 27 162 307 62 698 107
Cuts at: 2361 3527 4804 5158 482
Size: 1166 1277 354 2994
Fragments arranged by size:
2994 1277 1166 698 354 351 307 165
162 107 62 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1633 1804 1869 2641 2951 5253 5386 5845 6054
Size: 171 65 772 310 2302 133 459 209
Cuts at: 6054 1633
Size: 3249
Fragments arranged by size:
3249 2302 772 459 310 209 171 133
65
TatI w'GTAC_w
Cuts at: 239 7185 239
Size: 6946 724
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1159 1191 2689 2721 3567 3599 3709 3741 3970
Size: 32 1498 32 846 32 110 32 229
Cuts at: 3970 4002 7187 7219 1159
Size: 32 3185 32 1610
Fragments arranged by size:
3185 1610 1498 846 229 110 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1666 5467 1666
Size: 3801 3869
XbaI T'CTAG_A
Cuts at: 4701 4701
Size: 7670
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2424 2443 3937 4449 5165 2424
Size: 19 1494 512 716 4929
Fragments arranged by size:
4929 1494 716 512 19
XmaI C'CCGG_G
Cuts at: 789 1920 2263 2591 2880 3170 3785 3800 4031
Size: 1131 343 328 289 290 615 15 231
Cuts at: 4031 4690 789
Size: 659 3769
Fragments arranged by size:
3769 1131 659 615 343 328 290 289
231 15
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI FspAI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AscI BaeI BfrBI BmtI BsiWI BsmI
BsrGI BssHII BstEII BstZ17I ClaI EcoRV HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PshAI
PsiI PspXI PsrI SacII SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI