Restriction map for
pF1KA0288
(Circular) (Six-base) MAPSORT of: pF1KA0288.seq Check: 7251 from: 1 to: 6365
pF1KA0288 6365 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1767 2119 1767
Size: 352 6013
AccI GT'mk_AC
Cuts at: 772 3403 772
Size: 2631 3734
Acc65I G'GTAC_C
Cuts at: 2969 3381 2969
Size: 412 5953
AclI AA'CG_TT
Cuts at: 6205 6205
Size: 6365
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1860 2116 2497 2823 4190 4622 4975 5637 1860
Size: 256 381 326 1367 432 353 662 2588
Fragments arranged by size:
2588 1367 662 432 381 353 326 256
AflIII A'CryG_T
Cuts at: 2684 3717 5517 5798 2684
Size: 1033 1800 281 3251
Fragments arranged by size:
3251 1800 1033 281
AhdI GACnn_n'nnGTC
Cuts at: 1452 1452
Size: 6365
AleI CACnn'nnGTG
Cuts at: 616 616
Size: 6365
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 259 291 259
Size: 32 6333
AlwNI CAG_nnn'CTG
Cuts at: 318 486 534 561 844 1536 1545 1575 1611
Size: 168 48 27 283 692 9 30 36
Cuts at: 1611 1710 1869 2304 3670 5108 318
Size: 99 159 435 1366 1438 1575
Fragments arranged by size:
1575 1438 1366 692 435 283 168 159
99 48 36 30 27 9
ApaI G_GGCC'C
Cuts at: 2759 2759
Size: 6365
ApaLI G'TGCA_C
Cuts at: 2106 2248 2401 2629 5203 2106
Size: 142 153 228 2574 3268
Fragments arranged by size:
3268 2574 228 153 142
ApoI r'AATT_y
Cuts at: 637 3369 5718 5974 637
Size: 2732 2349 256 1028
Fragments arranged by size:
2732 2349 1028 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6365
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6365
AvaI C'yCGr_G
Cuts at: 1991 2134 3022 3385 1991
Size: 143 888 363 4971
Fragments arranged by size:
4971 888 363 143
BamHI G'GATC_C
Cuts at: 1910 2150 3390 1910
Size: 240 1240 4885
Fragments arranged by size:
4885 1240 240
BanI G'GyrC_C
Cuts at: 515 1276 2969 3381 3795 4043 4078 515
Size: 761 1693 412 414 248 35 2802
Fragments arranged by size:
2802 1693 761 414 412 248 35
BanII G_rGCy'C
Cuts at: 115 332 1694 1724 1793 1823 2045 2736 2759
Size: 217 1362 30 69 30 222 691 23
Cuts at: 2759 3338 3379 4409 115
Size: 579 41 1030 2071
Fragments arranged by size:
2071 1362 1030 691 579 222 217 69
41 30 30 23
BbeI G_GCGC'C
Cuts at: 3799 4047 3799
Size: 248 6117
BbsI GAAGACnn'nnnn_
Cuts at: 2445 2445
Size: 6365
BbvCI CC'TCA_GC
Cuts at: 1921 1921
Size: 6365
BciVI GTATCCnnnnn_n'
Cuts at: 4263 5319 6365 4263
Size: 1056 1046 4263
Fragments arranged by size:
4263 1056 1046
BclI T'GATC_A
Cuts at: 3885 3885
Size: 6365
BglI GCCn_nnn'nGGC
Cuts at: 241 1653 2192 2425 2999 241
Size: 1412 539 233 574 3607
Fragments arranged by size:
3607 1412 574 539 233
BglII A'GATC_T
Cuts at: 3880 5917 3880
Size: 2037 4328
BlpI GC'TnA_GC
Cuts at: 3477 3477
Size: 6365
Bme1580I G_kGCm'C
Cuts at: 251 1207 2110 2252 2405 2633 2752 2759 3990
Size: 956 903 142 153 228 119 7 1231
Cuts at: 3990 4083 5207 251
Size: 93 1124 1409
Fragments arranged by size:
1409 1231 1124 956 903 228 153 142
119 93 7
BmgBI CAC'GTC
Cuts at: 2628 2628
Size: 6365
BmrI ACTGGGnnnn_n'
Cuts at: 1497 1501 1850 2631 3577 3658 3993 6137 1497
Size: 4 349 781 946 81 335 2144 1725
Fragments arranged by size:
2144 1725 946 781 349 335 81 4
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 423 2421 4732 423
Size: 1998 2311 2056
Fragments arranged by size:
2311 2056 1998
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 529 556 1234 1387 1597 1918 2251 529
Size: 27 678 153 210 321 333 4643
Fragments arranged by size:
4643 678 333 321 210 153 27
Bpu10I CC'TnA_GC
Cuts at: 1297 1921 1297
Size: 624 5741
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 556 3541 4420 5134 5432 325
Size: 231 2985 879 714 298 1258
Fragments arranged by size:
2985 1258 879 714 298 231
BsaI GGTCTCn'nnnn_
Cuts at: 140 3315 6069 140
Size: 3175 2754 436
Fragments arranged by size:
3175 2754 436
BsaAI yAC'GTr
Cuts at: 3718 3718
Size: 6365
BsaBI GATnn'nnATC
Cuts at: 6 373 2611 3879 5916 6
Size: 367 2238 1268 2037 455
Fragments arranged by size:
2238 2037 1268 455 367
BsaHI Gr'CG_yC
Cuts at: 809 1223 1936 3056 3796 4044 4746 6242 809
Size: 414 713 1120 740 248 702 1496 932
Fragments arranged by size:
1496 1120 932 740 713 702 414 248
BsaWI w'CCGG_w
Cuts at: 671 1000 3554 4075 4753 5164 5311 5585 671
Size: 329 2554 521 678 411 147 274 1451
Fragments arranged by size:
2554 1451 678 521 411 329 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1009 1039 1403 1433 2539 2569 2935 2965 1009
Size: 30 364 30 1106 30 366 30 4409
Fragments arranged by size:
4409 1106 366 364 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1690 1699 1702 2248 2536 3346 3361 1690
Size: 9 3 546 288 810 15 4694
Fragments arranged by size:
4694 810 546 288 15 9 3
BseYI C'CCAG_C
Cuts at: 193 1006 1069 1173 2648 2671 3084 3790 4548
Size: 813 63 104 1475 23 413 706 758
Cuts at: 4548 5213 5621 193
Size: 665 408 937
Fragments arranged by size:
1475 937 813 758 706 665 413 408
104 63 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1002 1463 1795 2056 1002
Size: 461 332 261 5311
Fragments arranged by size:
5311 461 332 261
BsiEI CG_ry'CG
Cuts at: 104 1127 2861 3006 3907 3953 5183 104
Size: 1023 1734 145 901 46 1230 1286
Fragments arranged by size:
1734 1286 1230 1023 901 145 46
BsiHKAI G_wGCw'C
Cuts at: 115 332 440 1303 1413 1694 1703 2110 2141
Size: 217 108 863 110 281 9 407 31
Cuts at: 2141 2252 2405 2530 2633 3379 4157 4347 5207
Size: 111 153 125 103 746 778 190 860
Cuts at: 5207 115
Size: 1273
Fragments arranged by size:
1273 863 860 778 746 407 281 217
190 153 125 111 110 108 103 31
9
BsmBI CGTCTCn'nnnn_
Cuts at: 1078 1672 2173 2810 3262 1078
Size: 594 501 637 452 4181
Fragments arranged by size:
4181 637 594 501 452
Bsp1286I G_dGCh'C
Cuts at: 115 251 332 440 1207 1303 1413 1694 1703
Size: 136 81 108 767 96 110 281 9
Cuts at: 1703 1724 1793 1823 2045 2110 2141 2252 2405
Size: 21 69 30 222 65 31 111 153
Cuts at: 2405 2530 2633 2736 2752 2759 3338 3379 3990
Size: 125 103 103 16 7 579 41 611
Cuts at: 3990 4083 4157 4347 4409 5207 115
Size: 93 74 190 62 798 1273
Fragments arranged by size:
1273 798 767 611 579 281 222 190
153 136 125 111 110 108 103 103
96 93 81 74 69 65 62 41
31 30 21 16 9 7
BspEI T'CCGG_A
Cuts at: 1000 1000
Size: 6365
BspHI T'CATG_A
Cuts at: 4797 4797
Size: 6365
BspMI ACCTGCnnnn'nnnn_
Cuts at: 266 1767 2119 3415 3629 3931 4312 266
Size: 1501 352 1296 214 302 381 2319
Fragments arranged by size:
2319 1501 1296 381 352 302 214
BsrBI CCG'CTC
Cuts at: 50 764 1129 1317 4657 4711 6195 6360 50
Size: 714 365 188 3340 54 1484 165 55
Fragments arranged by size:
3340 1484 714 365 188 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4277 4277
Size: 6365
BsrFI r'CCGG_y
Cuts at: 297 1189 1377 1733 1768 1951 2294 2791 4363
Size: 892 188 356 35 183 343 497 1572
Cuts at: 4363 4544 297
Size: 181 2118
Fragments arranged by size:
2118 1572 892 497 356 343 188 183
181 35
BsrGI T'GTAC_A
Cuts at: 2681 2681
Size: 6365
BssSI C'ACGA_G
Cuts at: 397 418 433 1112 2074 4636 5344 397
Size: 21 15 679 962 2562 708 1418
Fragments arranged by size:
2562 1418 962 708 679 21 15
BstAPI GCAn_nnn'nTGC
Cuts at: 318 486 561 1611 1869 3742 318
Size: 168 75 1050 258 1873 2941
Fragments arranged by size:
2941 1873 1050 258 168 75
BstBI TT'CG_AA
Cuts at: 4726 4726
Size: 6365
BstEII G'GTnAC_C
Cuts at: 3273 3273
Size: 6365
BstXI CCAn_nnnn'nTGG
Cuts at: 130 201 130
Size: 71 6294
BstYI r'GATC_y
Cuts at: 1 356 368 1910 2150 2606 3173 3390 3857
Size: 355 12 1542 240 456 567 217 467
Cuts at: 3857 3880 4215 4461 4767 4779 4865 5917 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1542 1052 567 467 456 449 355 335
306 246 240 217 86 23 12 12
BtgI C'CryG_G
Cuts at: 212 251 602 1825 1940 2211 2534 2558 2564
Size: 39 351 1223 115 271 323 24 6
Cuts at: 2564 2634 2810 2912 3155 3281 3296 3338 212
Size: 70 176 102 243 126 15 42 3239
Fragments arranged by size:
3239 1223 351 323 271 243 176 126
115 102 70 42 39 24 15 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 360 1133 1817 4315 4496 4659 360
Size: 773 684 2498 181 163 2066
Fragments arranged by size:
2498 2066 773 684 181 163
BtsI GCAGTG_nn'
Cuts at: 251 280 684 938 1411 3608 251
Size: 29 404 254 473 2197 3008
Fragments arranged by size:
3008 2197 473 404 254 29
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2916 2951 2916
Size: 35 6330
DraI TTT'AAA
Cuts at: 3364 3364
Size: 6365
DrdI GACnn_nn'nnGTC
Cuts at: 2674 4071 5415 2674
Size: 1397 1344 3624
Fragments arranged by size:
3624 1397 1344
EaeI y'GGCC_r
Cuts at: 141 209 464 605 887 1124 1974 2462 2561
Size: 68 255 141 282 237 850 488 99
Cuts at: 2561 3003 3950 4124 4515 4542 5887 141
Size: 442 947 174 391 27 1345 619
Fragments arranged by size:
1345 947 850 619 488 442 391 282
255 237 174 141 99 68 27
EagI C'GGCC_G
Cuts at: 1124 3003 3950 1124
Size: 1879 947 3539
Fragments arranged by size:
3539 1879 947
EarI CTCTTCn'nnn_
Cuts at: 1349 1805 1879 3338 4388 4598 1349
Size: 456 74 1459 1050 210 3116
Fragments arranged by size:
3116 1459 1050 456 210 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1096 1512 2488 2943 4573 5315 5461 6175 1096
Size: 416 976 455 1630 742 146 714 1286
Fragments arranged by size:
1630 1286 976 742 714 455 416 146
EcoICRI GAG'CTC
Cuts at: 113 330 1692 3377 113
Size: 217 1362 1685 3101
Fragments arranged by size:
3101 1685 1362 217
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 529 556 1234 1387 1597 1860 1918 2116 2251
Size: 27 678 153 210 263 58 198 135
Cuts at: 2251 2497 2823 4190 4622 4975 5637 529
Size: 246 326 1367 432 353 662 1257
Fragments arranged by size:
1367 1257 678 662 432 353 326 263
246 210 198 153 135 58 27
EcoO109I rG'GnC_Cy
Cuts at: 2645 2755 3504 2645
Size: 110 749 5506
Fragments arranged by size:
5506 749 110
EcoRI G'AATT_C
Cuts at: 3369 3369
Size: 6365
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2502 4384 2502
Size: 1882 4483
FspI TGC'GCA
Cuts at: 4146 4146
Size: 6365
HaeII r_GCGC'y
Cuts at: 220 340 664 992 1851 3195 3799 4047 5277
Size: 120 324 328 859 1344 604 248 1230
Cuts at: 5277 6048 220
Size: 771 537
Fragments arranged by size:
1344 1230 859 771 604 537 328 324
248 120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1438 1470 3225 3257 4207 4237 4239 4269 1438
Size: 32 1755 32 950 30 2 30 3534
Fragments arranged by size:
3534 1755 950 32 32 30 30 2
HincII GTy'rAC
Cuts at: 773 2778 3404 773
Size: 2005 626 3734
Fragments arranged by size:
3734 2005 626
HindIII A'AGCT_T
Cuts at: 2575 2575
Size: 6365
KasI G'GCGC_C
Cuts at: 3795 4043 3795
Size: 248 6117
KpnI G_GTAC'C
Cuts at: 2973 3385 2973
Size: 412 5953
MluI A'CGCG_T
Cuts at: 5798 5798
Size: 6365
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 941 5124 5308 941
Size: 4183 184 1998
Fragments arranged by size:
4183 1998 184
MscI TGG'CCA
Cuts at: 211 466 607 2464 4126 211
Size: 255 141 1857 1662 2450
Fragments arranged by size:
2450 1857 1662 255 141
MslI CAynn'nnrTG
Cuts at: 128 616 1960 2089 2533 3280 4354 4481 128
Size: 488 1344 129 444 747 1074 127 2012
Fragments arranged by size:
2012 1344 1074 747 488 444 129 127
MspA1I CmG'CkG
Cuts at: 318 501 1515 1908 1920 2213 2729 2985 3220
Size: 183 1014 393 12 293 516 256 235
Cuts at: 3220 3476 3790 4150 4934 5179 318
Size: 256 314 360 784 245 1504
Fragments arranged by size:
1504 1014 784 516 393 360 314 293
256 256 245 235 183 12
NaeI GCC'GGC
Cuts at: 299 1379 1770 1953 4546 299
Size: 1080 391 183 2593 2118
Fragments arranged by size:
2593 2118 1080 391 183
NarI GG'CG_CC
Cuts at: 3796 4044 3796
Size: 248 6117
NcoI C'CATG_G
Cuts at: 251 1940 2534 2634 2810 3155 3281 3338 251
Size: 1689 594 100 176 345 126 57 3278
Fragments arranged by size:
3278 1689 594 345 176 126 100 57
NdeI CA'TA_TG
Cuts at: 2389 2389
Size: 6365
NgoMIV G'CCGG_C
Cuts at: 297 1377 1768 1951 4544 297
Size: 1080 391 183 2593 2118
Fragments arranged by size:
2593 2118 1080 391 183
NspI r_CATG'y
Cuts at: 192 2688 3071 3418 3918 5521 192
Size: 2496 383 347 500 1603 1036
Fragments arranged by size:
2496 1603 1036 500 383 347
PciI A'CATG_T
Cuts at: 2684 5517 2684
Size: 2833 3532
PflMI CCAn_nnn'nTGG
Cuts at: 156 156
Size: 6365
PfoI T'CCnGG_A
Cuts at: 1027 1027
Size: 6365
PmeI GTTT'AAAC
Cuts at: 3364 3364
Size: 6365
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4811 4843 4811
Size: 32 6333
PpuMI rG'GwC_Cy
Cuts at: 2645 2645
Size: 6365
PspOMI G'GGCC_C
Cuts at: 2755 2755
Size: 6365
PspXI vC'TCGA_Gb
Cuts at: 3022 3022
Size: 6365
PstI C_TGCA'G
Cuts at: 323 566 1352 1541 1616 2177 2585 3203 3412
Size: 243 786 189 75 561 408 618 209
Cuts at: 3412 4097 323
Size: 685 2591
Fragments arranged by size:
2591 786 685 618 561 408 243 209
189 75
PvuI CG_AT'CG
Cuts at: 104 2861 104
Size: 2757 3608
PvuII CAG'CTG
Cuts at: 318 1920 2985 3790 4150 318
Size: 1602 1065 805 360 2533
Fragments arranged by size:
2533 1602 1065 805 360
RsrII CG'GwC_CG
Cuts at: 4560 4560
Size: 6365
SacI G_AGCT'C
Cuts at: 115 332 1694 3379 115
Size: 217 1362 1685 3101
Fragments arranged by size:
3101 1685 1362 217
SacII CC_GC'GG
Cuts at: 2214 2214
Size: 6365
SalI G'TCGA_C
Cuts at: 771 3402 771
Size: 2631 3734
SanDI GG'GwC_CC
Cuts at: 2645 2645
Size: 6365
SapI GCTCTTCn'nnn_
Cuts at: 1879 4388 4598 1879
Size: 2509 210 3646
Fragments arranged by size:
3646 2509 210
SbfI CC_TGCA'GG
Cuts at: 2177 3412 2177
Size: 1235 5130
ScaI AGT'ACT
Cuts at: 2827 3187 5882 2827
Size: 360 2695 3310
Fragments arranged by size:
3310 2695 360
SexAI A'CCwGG_T
Cuts at: 1544 1544
Size: 6365
SfcI C'TryA_G
Cuts at: 33 319 562 1348 1537 1612 2173 2239 2581
Size: 286 243 786 189 75 561 66 342
Cuts at: 2581 2661 3199 3408 4093 5061 5252 33
Size: 80 538 209 685 968 191 1146
Fragments arranged by size:
1146 968 786 685 561 538 342 286
243 209 191 189 80 75 66
SfoI GGC'GCC
Cuts at: 3797 4045 3797
Size: 248 6117
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6365
SgrAI Cr'CCGG_yG
Cuts at: 2791 2791
Size: 6365
SmaI CCC'GGG
Cuts at: 1993 3387 1993
Size: 1394 4971
SmlI C'TyrA_G
Cuts at: 340 571 3022 3520 4435 5149 5411 340
Size: 231 2451 498 915 714 262 1294
Fragments arranged by size:
2451 1294 915 714 498 262 231
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6365
SphI G_CATG'C
Cuts at: 3418 3918 3418
Size: 500 5865
StuI AGG'CCT
Cuts at: 1403 1983 2072 1403
Size: 580 89 5696
Fragments arranged by size:
5696 580 89
StyI C'CwwG_G
Cuts at: 251 1310 1433 1940 2534 2634 2810 3155 3281
Size: 1059 123 507 594 100 176 345 126
Cuts at: 3281 3338 3499 3853 251
Size: 57 161 354 2763
Fragments arranged by size:
2763 1059 594 507 354 345 176 161
126 123 100 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1029 1294 3948 4081 4540 4749 1029
Size: 265 2654 133 459 209 2645
Fragments arranged by size:
2654 2645 459 265 209 133
TatI w'GTAC_w
Cuts at: 2681 2825 3185 5880 2681
Size: 144 360 2695 3166
Fragments arranged by size:
3166 2695 360 144
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 676 708 1403 1414 1435 1446 5882 5914 676
Size: 32 695 11 21 11 4436 32 1127
Fragments arranged by size:
4436 1127 695 32 32 21 11 11
Tth111I GACn'n_nGTC
Cuts at: 1503 4162 1503
Size: 2659 3706
XbaI T'CTAG_A
Cuts at: 3396 3396
Size: 6365
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1170 3860 1170
Size: 2690 3675
XhoI C'TCGA_G
Cuts at: 3022 3022
Size: 6365
XmaI C'CCGG_G
Cuts at: 1991 3385 1991
Size: 1394 4971
XmnI GAAnn'nnTTC
Cuts at: 2716 2716
Size: 6365
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AlfI AscI AvrII BaeI
BcgI BfrBI BmtI BsiWI BsmI BssHII BstZ17I Bsu36I
ClaI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI
NheI NotI NruI NsiI PacI PasI PmlI PshAI
PsiI PsrI SfiI SnaBI SrfI SspI SwaI ZraI