Restriction map for
pF1KA0291
(Circular) (Six-base) MAPSORT of: pF1KA0291.seq Check: 5035 from: 1 to: 6146
pF1KA0291 6146 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3009 3009
Size: 6146
AccI GT'mk_AC
Cuts at: 3184 3184
Size: 6146
Acc65I G'GTAC_C
Cuts at: 3162 3162
Size: 6146
AclI AA'CG_TT
Cuts at: 5986 5986
Size: 6146
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 1210 1735 1807 1969 2635 3971 4403 4756
Size: 1062 525 72 162 666 1336 432 353
Cuts at: 4756 5418 148
Size: 662 876
Fragments arranged by size:
1336 1062 876 666 662 525 432 353
162 72
AflIII A'CryG_T
Cuts at: 3498 5298 5579 3498
Size: 1800 281 4065
Fragments arranged by size:
4065 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1046 1046
Size: 6146
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1138 1343 1138
Size: 205 5941
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1429 1461 1429
Size: 32 6114
AlwNI CAG_nnn'CTG
Cuts at: 283 399 612 1221 1578 2706 3451 4889 283
Size: 116 213 609 357 1128 745 1438 1540
Fragments arranged by size:
1540 1438 1128 745 609 357 213 116
ApaI G_GGCC'C
Cuts at: 304 1918 2118 2556 304
Size: 1614 200 438 3894
Fragments arranged by size:
3894 1614 438 200
ApaLI G'TGCA_C
Cuts at: 2197 4984 2197
Size: 2787 3359
ApoI r'AATT_y
Cuts at: 3150 5499 5755 3150
Size: 2349 256 3541
Fragments arranged by size:
3541 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6146
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6146
AvaI C'yCGr_G
Cuts at: 289 567 1161 1587 1911 2676 2944 3166 289
Size: 278 594 426 324 765 268 222 3269
Fragments arranged by size:
3269 765 594 426 324 278 268 222
BamHI G'GATC_C
Cuts at: 715 3171 715
Size: 2456 3690
BanI G'GyrC_C
Cuts at: 904 1165 1921 2627 2940 3162 3576 3824 3859
Size: 261 756 706 313 222 414 248 35
Cuts at: 3859 904
Size: 3191
Fragments arranged by size:
3191 756 706 414 313 261 248 222
35
BanII G_rGCy'C
Cuts at: 304 568 875 1359 1538 1568 1918 2118 2556
Size: 264 307 484 179 30 350 200 438
Cuts at: 2556 3160 4190 304
Size: 604 1030 2260
Fragments arranged by size:
2260 1030 604 484 438 350 307 264
200 179 30
BbeI G_GCGC'C
Cuts at: 1925 2631 2944 3580 3828 1925
Size: 706 313 636 248 4243
Fragments arranged by size:
4243 706 636 313 248
BbsI GAAGACnn'nnnn_
Cuts at: 703 805 1012 2668 703
Size: 102 207 1656 4181
Fragments arranged by size:
4181 1656 207 102
BbvCI CC'TCA_GC
Cuts at: 1247 1247
Size: 6146
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 6112
BciVI GTATCCnnnnn_n'
Cuts at: 4044 5100 6146 4044
Size: 1056 1046 4044
Fragments arranged by size:
4044 1056 1046
BclI T'GATC_A
Cuts at: 3666 3666
Size: 6146
BglI GCCn_nnn'nGGC
Cuts at: 167 1118 1717 1726 167
Size: 951 599 9 4587
Fragments arranged by size:
4587 951 599 9
BglII A'GATC_T
Cuts at: 1157 2267 3661 5698 1157
Size: 1110 1394 2037 1605
Fragments arranged by size:
2037 1605 1394 1110
BlpI GC'TnA_GC
Cuts at: 3014 3258 3014
Size: 244 5902
Bme1580I G_kGCm'C
Cuts at: 304 505 631 907 925 1168 1918 2118 2201
Size: 201 126 276 18 243 750 200 83
Cuts at: 2201 2556 3771 3864 4988 304
Size: 355 1215 93 1124 1462
Fragments arranged by size:
1462 1215 1124 750 355 276 243 201
200 126 93 83 18
BmgBI CAC'GTC
Cuts at: 2220 2220
Size: 6146
BmrI ACTGGGnnnn_n'
Cuts at: 191 3358 3439 3774 5918 191
Size: 3167 81 335 2144 419
Fragments arranged by size:
3167 2144 419 335 81
BmtI G_CTAG'C
Cuts at: 218 218
Size: 6146
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2286 4513 2286
Size: 2227 3919
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 141 496 1432 1504 1546 1552 1906 2008 2041
Size: 355 936 72 42 6 354 102 33
Cuts at: 2041 2143 2170 2368 2590 2653 2665 2773 141
Size: 102 27 198 222 63 12 108 3514
Fragments arranged by size:
3514 936 355 354 222 198 108 102
102 72 63 42 33 27 12 6
Bpu10I CC'TnA_GC
Cuts at: 1247 2548 2791 1247
Size: 1301 243 4602
Fragments arranged by size:
4602 1301 243
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1288 2242 2824 3322 4201 4915 5213 1288
Size: 954 582 498 879 714 298 2221
Fragments arranged by size:
2221 954 879 714 582 498 298
BsaI GGTCTCn'nnnn_
Cuts at: 943 1126 1226 1472 2017 2641 5850 943
Size: 183 100 246 545 624 3209 1239
Fragments arranged by size:
3209 1239 624 545 246 183 100
BsaAI yAC'GTr
Cuts at: 819 2037 3499 819
Size: 1218 1462 3466
Fragments arranged by size:
3466 1462 1218
BsaBI GATnn'nnATC
Cuts at: 6 967 3660 5697 6
Size: 961 2693 2037 455
Fragments arranged by size:
2693 2037 961 455
BsaHI Gr'CG_yC
Cuts at: 1616 1922 2606 2628 2672 2941 2987 3577 3825
Size: 306 684 22 44 269 46 590 248
Cuts at: 3825 4527 6023 1616
Size: 702 1496 1739
Fragments arranged by size:
1739 1496 702 684 590 306 269 248
46 44 22
BsaWI w'CCGG_w
Cuts at: 1602 3082 3335 3856 4534 4945 5092 5366 1602
Size: 1480 253 521 678 411 147 274 2382
Fragments arranged by size:
2382 1480 678 521 411 274 253 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1447 1477 2563 2593 2638 2668 1447
Size: 30 1086 30 45 30 4925
Fragments arranged by size:
4925 1086 45 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 252 255 1080 1354 1429 1432 1437 1471 1474
Size: 3 825 274 75 3 5 34 3
Cuts at: 1474 1543 1546 1576 2098 2397 2464 3060 252
Size: 69 3 30 522 299 67 596 3338
Fragments arranged by size:
3338 825 596 522 299 274 75 69
67 34 30 5 3 3 3 3
BseYI C'CCAG_C
Cuts at: 118 990 1051 1108 1925 1992 2092 2117 2411
Size: 872 61 57 817 67 100 25 294
Cuts at: 2411 3571 4329 4994 5402 118
Size: 1160 758 665 408 862
Fragments arranged by size:
1160 872 862 817 758 665 408 294
100 67 61 57 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 412 223
Size: 189 5957
BsiEI CG_ry'CG
Cuts at: 104 141 275 560 2393 3688 3734 4964 104
Size: 37 134 285 1833 1295 46 1230 1286
Fragments arranged by size:
1833 1295 1286 1230 285 134 46 37
BsiHKAI G_wGCw'C
Cuts at: 1538 1568 1982 2201 2407 2980 3160 3938 4128
Size: 30 414 219 206 573 180 778 190
Cuts at: 4128 4988 1538
Size: 860 2696
Fragments arranged by size:
2696 860 778 573 414 219 206 190
180 30
BsmI GAATG_Cn'
Cuts at: 621 621
Size: 6146
BsmBI CGTCTCn'nnnn_
Cuts at: 400 2446 400
Size: 2046 4100
Bsp1286I G_dGCh'C
Cuts at: 304 505 568 631 875 907 925 1168 1359
Size: 201 63 63 244 32 18 243 191
Cuts at: 1359 1538 1568 1918 1982 2118 2201 2407 2556
Size: 179 30 350 64 136 83 206 149
Cuts at: 2556 2980 3160 3771 3864 3938 4128 4190 4988
Size: 424 180 611 93 74 190 62 798
Cuts at: 4988 304
Size: 1462
Fragments arranged by size:
1462 798 611 424 350 244 243 206
201 191 190 180 179 149 136 93
83 74 64 63 63 62 32 30
18
BspEI T'CCGG_A
Cuts at: 1602 1602
Size: 6146
BspHI T'CATG_A
Cuts at: 4578 4578
Size: 6146
BspMI ACCTGCnnnn'nnnn_
Cuts at: 770 3009 3196 3410 3712 4093 770
Size: 2239 187 214 302 381 2823
Fragments arranged by size:
2823 2239 381 302 214 187
BsrBI CCG'CTC
Cuts at: 50 357 1644 2304 2721 4438 4492 5976 6141
Size: 307 1287 660 417 1717 54 1484 165
Cuts at: 6141 50
Size: 55
Fragments arranged by size:
1717 1484 1287 660 417 307 165 55
54
BsrDI GCAATG_nn'
Cuts at: 616 4058 616
Size: 3442 2704
BsrFI r'CCGG_y
Cuts at: 700 2144 4144 4325 700
Size: 1444 2000 181 2521
Fragments arranged by size:
2521 2000 1444 181
BssSI C'ACGA_G
Cuts at: 1366 2200 4417 5125 1366
Size: 834 2217 708 2387
Fragments arranged by size:
2387 2217 834 708
BstAPI GCAn_nnn'nTGC
Cuts at: 283 3523 283
Size: 3240 2906
BstBI TT'CG_AA
Cuts at: 4507 4507
Size: 6146
BstXI CCAn_nnnn'nTGG
Cuts at: 165 433 1017 1410 165
Size: 268 584 393 4901
Fragments arranged by size:
4901 584 393 268
BstYI r'GATC_y
Cuts at: 1 715 1157 1437 1625 2267 3171 3638 3661
Size: 714 442 280 188 642 904 467 23
Cuts at: 3661 3996 4242 4548 4560 4646 5698 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 904 714 642 467 449 442 335
306 280 246 188 86 23 12
BtgI C'CryG_G
Cuts at: 141 164 554 587 1022 1094 1169 141
Size: 23 390 33 435 72 75 5118
Fragments arranged by size:
5118 435 390 75 72 33 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4096 4277 4440 4096
Size: 181 163 5802
Fragments arranged by size:
5802 181 163
BtsI GCAGTG_nn'
Cuts at: 235 1122 1579 1961 3389 235
Size: 887 457 382 1428 2992
Fragments arranged by size:
2992 1428 887 457 382
DraI TTT'AAA
Cuts at: 3145 3145
Size: 6146
DraIII CAC_nnn'GTG
Cuts at: 588 960 588
Size: 372 5774
DrdI GACnn_nn'nnGTC
Cuts at: 3852 5196 3852
Size: 1344 4802
EaeI y'GGCC_r
Cuts at: 138 209 272 557 632 1019 1253 1499 1655
Size: 71 63 285 75 387 234 246 156
Cuts at: 1655 1751 2276 3731 3905 4296 4323 5668 138
Size: 96 525 1455 174 391 27 1345 616
Fragments arranged by size:
1455 1345 616 525 391 387 285 246
234 174 156 96 75 71 63 27
EagI C'GGCC_G
Cuts at: 138 272 557 3731 138
Size: 134 285 3174 2553
Fragments arranged by size:
3174 2553 285 134
EarI CTCTTCn'nnn_
Cuts at: 946 1143 4169 4379 946
Size: 197 3026 210 2713
Fragments arranged by size:
3026 2713 210 197
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 328 1744 2835 4354 5096 5242 5956 328
Size: 1416 1091 1519 742 146 714 518
Fragments arranged by size:
1519 1416 1091 742 714 518 146
EcoICRI GAG'CTC
Cuts at: 1536 1566 3158 1536
Size: 30 1592 4524
Fragments arranged by size:
4524 1592 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 141 148 496 1210 1432 1504 1546 1552 1735
Size: 7 348 714 222 72 42 6 183
Cuts at: 1735 1807 1906 1969 2008 2041 2143 2170 2368
Size: 72 99 63 39 33 102 27 198
Cuts at: 2368 2590 2635 2653 2665 2773 3971 4403 4756
Size: 222 45 18 12 108 1198 432 353
Cuts at: 4756 5418 141
Size: 662 869
Fragments arranged by size:
1198 869 714 662 432 353 348 222
222 198 183 108 102 99 72 72
63 45 42 39 33 27 18 12
7 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1303 2614 1303
Size: 1311 4835
EcoO109I rG'GnC_Cy
Cuts at: 301 759 1591 1710 1890 2052 2397 2481 2552
Size: 458 832 119 180 162 345 84 71
Cuts at: 2552 2787 2856 3285 301
Size: 235 69 429 3162
Fragments arranged by size:
3162 832 458 429 345 235 180 162
119 84 71 69
EcoRI G'AATT_C
Cuts at: 3150 3150
Size: 6146
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4165 4165
Size: 6146
FspI TGC'GCA
Cuts at: 3927 3927
Size: 6146
HaeII r_GCGC'y
Cuts at: 1925 2178 2631 2916 2944 3580 3828 5058 5829
Size: 253 453 285 28 636 248 1230 771
Cuts at: 5829 1925
Size: 2242
Fragments arranged by size:
2242 1230 771 636 453 285 253 248
28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 568 600 700 707 721 732 739 753 1032
Size: 32 100 7 14 11 7 14 279
Cuts at: 1032 1064 1447 1479 1541 1573 2638 2670 2920
Size: 32 383 32 62 32 1065 32 250
Cuts at: 2920 2952 3988 4018 4020 4050 568
Size: 32 1036 30 2 30 2664
Fragments arranged by size:
2664 1065 1036 383 279 250 100 62
32 32 32 32 32 32 30 30
14 14 11 7 7 2
HincII GTy'rAC
Cuts at: 3185 3185
Size: 6146
KasI G'GCGC_C
Cuts at: 1921 2627 2940 3576 3824 1921
Size: 706 313 636 248 4243
Fragments arranged by size:
4243 706 636 313 248
KpnI G_GTAC'C
Cuts at: 3166 3166
Size: 6146
MluI A'CGCG_T
Cuts at: 5579 5579
Size: 6146
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 743 1427 2273 2882 4905 5089 743
Size: 684 846 609 2023 184 1800
Fragments arranged by size:
2023 1800 846 684 609 184
MscI TGG'CCA
Cuts at: 1021 1255 3907 1021
Size: 234 2652 3260
Fragments arranged by size:
3260 2652 234
MslI CAynn'nnrTG
Cuts at: 1027 1972 2035 2422 4135 4262 1027
Size: 945 63 387 1713 127 2911
Fragments arranged by size:
2911 1713 945 387 127 63
MspA1I CmG'CkG
Cuts at: 122 280 645 734 1218 1410 1524 1986 2103
Size: 158 365 89 484 192 114 462 117
Cuts at: 2103 2121 2313 2970 3257 3571 3931 4715 4960
Size: 18 192 657 287 314 360 784 245
Cuts at: 4960 122
Size: 1308
Fragments arranged by size:
1308 784 657 484 462 365 360 314
287 245 192 192 158 117 114 89
18
NaeI GCC'GGC
Cuts at: 4327 4327
Size: 6146
NarI GG'CG_CC
Cuts at: 1922 2628 2941 3577 3825 1922
Size: 706 313 636 248 4243
Fragments arranged by size:
4243 706 636 313 248
NcoI C'CATG_G
Cuts at: 164 1022 164
Size: 858 5288
NgoMIV G'CCGG_C
Cuts at: 4325 4325
Size: 6146
NheI G'CTAG_C
Cuts at: 214 214
Size: 6146
NspI r_CATG'y
Cuts at: 3199 3699 5302 3199
Size: 500 1603 4043
Fragments arranged by size:
4043 1603 500
PasI CC'CwG_GG
Cuts at: 2325 2773 2325
Size: 448 5698
PciI A'CATG_T
Cuts at: 5298 5298
Size: 6146
PflMI CCAn_nnn'nTGG
Cuts at: 588 588
Size: 6146
PfoI T'CCnGG_A
Cuts at: 2656 2656
Size: 6146
PmeI GTTT'AAAC
Cuts at: 3145 3145
Size: 6146
PmlI CAC'GTG
Cuts at: 2037 2037
Size: 6146
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 397 429 4592 4624 397
Size: 32 4163 32 1919
Fragments arranged by size:
4163 1919 32 32
PpuMI rG'GwC_Cy
Cuts at: 759 1591 1890 2787 759
Size: 832 299 897 4118
Fragments arranged by size:
4118 897 832 299
PspOMI G'GGCC_C
Cuts at: 300 1914 2114 2552 300
Size: 1614 200 438 3894
Fragments arranged by size:
3894 1614 438 200
PstI C_TGCA'G
Cuts at: 1352 1445 1523 2072 2102 2159 2312 2489 2963
Size: 93 78 549 30 57 153 177 474
Cuts at: 2963 3193 3878 1352
Size: 230 685 3620
Fragments arranged by size:
3620 685 549 474 230 177 153 93
78 57 30
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6146
PvuII CAG'CTG
Cuts at: 280 1218 1410 1524 1986 2121 2970 3571 3931
Size: 938 192 114 462 135 849 601 360
Cuts at: 3931 280
Size: 2495
Fragments arranged by size:
2495 938 849 601 462 360 192 135
114
RsrII CG'GwC_CG
Cuts at: 4341 4341
Size: 6146
SacI G_AGCT'C
Cuts at: 1538 1568 3160 1538
Size: 30 1592 4524
Fragments arranged by size:
4524 1592 30
SalI G'TCGA_C
Cuts at: 3183 3183
Size: 6146
SanDI GG'GwC_CC
Cuts at: 1591 1591
Size: 6146
SapI GCTCTTCn'nnn_
Cuts at: 4169 4379 4169
Size: 210 5936
SbfI CC_TGCA'GG
Cuts at: 2489 3193 2489
Size: 704 5442
ScaI AGT'ACT
Cuts at: 5663 5663
Size: 6146
SexAI A'CCwGG_T
Cuts at: 907 907
Size: 6146
SfcI C'TryA_G
Cuts at: 33 1348 1441 1519 2068 2098 2155 2308 2485
Size: 1315 93 78 549 30 57 153 177
Cuts at: 2485 2959 3189 3874 4842 5033 33
Size: 474 230 685 968 191 1146
Fragments arranged by size:
1315 1146 968 685 549 474 230 191
177 153 93 78 57 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 1717 1717
Size: 6146
SfoI GGC'GCC
Cuts at: 1923 2629 2942 3578 3826 1923
Size: 706 313 636 248 4243
Fragments arranged by size:
4243 706 636 313 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6146
SmaI CCC'GGG
Cuts at: 3168 3168
Size: 6146
SmlI C'TyrA_G
Cuts at: 1303 2257 2839 3301 4216 4930 5192 1303
Size: 954 582 462 915 714 262 2257
Fragments arranged by size:
2257 954 915 714 582 462 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6146
SphI G_CATG'C
Cuts at: 3199 3699 3199
Size: 500 5646
StuI AGG'CCT
Cuts at: 1720 1720
Size: 6146
StyI C'CwwG_G
Cuts at: 164 221 305 839 876 995 1022 1256 1472
Size: 57 84 534 37 119 27 234 216
Cuts at: 1472 3280 3634 164
Size: 1808 354 2676
Fragments arranged by size:
2676 1808 534 354 234 216 119 84
57 37 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 354 942 1015 1625 2434 2554 3729 3862 4321
Size: 588 73 610 809 120 1175 133 459
Cuts at: 4321 4530 354
Size: 209 1970
Fragments arranged by size:
1970 1175 809 610 588 459 209 133
120 73
TatI w'GTAC_w
Cuts at: 5661 5661
Size: 6146
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1864 1896 2563 2595 5663 5695 1864
Size: 32 667 32 3068 32 2315
Fragments arranged by size:
3068 2315 667 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2652 3943 2652
Size: 1291 4855
XbaI T'CTAG_A
Cuts at: 3177 3177
Size: 6146
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1479 1678 1818 2016 3641 1479
Size: 199 140 198 1625 3984
Fragments arranged by size:
3984 1625 199 198 140
XmaI C'CCGG_G
Cuts at: 3166 3166
Size: 6146
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AleI AscI AvrII BaeI
BfrBI BsiWI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NotI NruI NsiI PacI PshAI PsiI PspXI PsrI
SacII SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI