Restriction map for
pF1KA0292
(Circular) (Six-base) MAPSORT of: pF1KA0292.seq Check: 2300 from: 1 to: 8993
pF1KA0292 8993 bp
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AccI GT'mk_AC
Cuts at: 1099 6031 1099
Size: 4932 4061
Acc65I G'GTAC_C
Cuts at: 1434 6009 1434
Size: 4575 4418
AclI AA'CG_TT
Cuts at: 8833 8833
Size: 8993
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1182 1631 1649 1660 2362 3962 4307 4391 4661
Size: 449 18 11 702 1600 345 84 270
Cuts at: 4661 5585 6818 7250 7603 8265 1182
Size: 924 1233 432 353 662 1910
Fragments arranged by size:
1910 1600 1233 924 702 662 449 432
353 345 270 84 18 11
AfeI AGC'GCT
Cuts at: 5741 5741
Size: 8993
AflIII A'CryG_T
Cuts at: 1523 6345 8145 8426 1523
Size: 4822 1800 281 2090
Fragments arranged by size:
4822 2090 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 2734 2734
Size: 8993
AleI CACnn'nnGTG
Cuts at: 2771 5369 2771
Size: 2598 6395
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1614 5387 1614
Size: 3773 5220
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3399 3431 3399
Size: 32 8961
AlwNI CAG_nnn'CTG
Cuts at: 298 301 687 1459 2593 4471 4968 5545 6298
Size: 3 386 772 1134 1878 497 577 753
Cuts at: 6298 7736 298
Size: 1438 1555
Fragments arranged by size:
1878 1555 1438 1134 772 753 577 497
386 3
ApaI G_GGCC'C
Cuts at: 2176 2563 4881 2176
Size: 387 2318 6288
Fragments arranged by size:
6288 2318 387
ApaLI G'TGCA_C
Cuts at: 7831 7831
Size: 8993
ApoI r'AATT_y
Cuts at: 225 1002 1337 1605 2350 3345 5659 5997 8346
Size: 777 335 268 745 995 2314 338 2349
Cuts at: 8346 8602 225
Size: 256 616
Fragments arranged by size:
2349 2314 995 777 745 616 338 335
268 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8993
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8993
AvaI C'yCGr_G
Cuts at: 1934 3308 6013 1934
Size: 1374 2705 4914
Fragments arranged by size:
4914 2705 1374
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3358 3391 3358
Size: 33 8960
BamHI G'GATC_C
Cuts at: 6018 6018
Size: 8993
BanI G'GyrC_C
Cuts at: 1434 4492 5210 5471 5788 5891 6009 6423 6671
Size: 3058 718 261 317 103 118 414 248
Cuts at: 6671 6706 1434
Size: 35 3721
Fragments arranged by size:
3721 3058 718 414 317 261 248 118
103 35
BanII G_rGCy'C
Cuts at: 2176 2563 2754 3315 3511 4881 5525 6007 7037
Size: 387 191 561 196 1370 644 482 1030
Cuts at: 7037 2176
Size: 4132
Fragments arranged by size:
4132 1370 1030 644 561 482 387 196
191
BbeI G_GCGC'C
Cuts at: 5475 5792 6427 6675 5475
Size: 317 635 248 7793
Fragments arranged by size:
7793 635 317 248
BbsI GAAGACnn'nnnn_
Cuts at: 809 1564 1918 2906 4397 4450 5189 5492 809
Size: 755 354 988 1491 53 739 303 4310
Fragments arranged by size:
4310 1491 988 755 739 354 303 53
BbvCI CC'TCA_GC
Cuts at: 428 3779 4790 5063 5962 428
Size: 3351 1011 273 899 3459
Fragments arranged by size:
3459 3351 1011 899 273
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 100 124 134 90
Size: 10 24 10 8949
Fragments arranged by size:
8949 24 10 10
BciVI GTATCCnnnnn_n'
Cuts at: 2297 6891 7947 8993 2297
Size: 4594 1056 1046 2297
Fragments arranged by size:
4594 2297 1056 1046
BclI T'GATC_A
Cuts at: 6513 6513
Size: 8993
BfrBI ATG'CAT
Cuts at: 2056 2056
Size: 8993
BglI GCCn_nnn'nGGC
Cuts at: 444 2156 3072 3583 4624 444
Size: 1712 916 511 1041 4813
Fragments arranged by size:
4813 1712 1041 916 511
BglII A'GATC_T
Cuts at: 6508 8545 6508
Size: 2037 6956
BlpI GC'TnA_GC
Cuts at: 6105 6105
Size: 8993
Bme1580I G_kGCm'C
Cuts at: 2176 2563 3579 4881 5213 5594 5896 5923 6618
Size: 387 1016 1302 332 381 302 27 695
Cuts at: 6618 6711 7835 2176
Size: 93 1124 3334
Fragments arranged by size:
3334 1302 1124 1016 695 387 381 332
302 93 27
BmrI ACTGGGnnnn_n'
Cuts at: 587 1292 1379 2565 2770 3566 3896 6205 6286
Size: 705 87 1186 205 796 330 2309 81
Cuts at: 6286 6621 8765 587
Size: 335 2144 815
Fragments arranged by size:
2309 2144 1186 815 796 705 335 330
205 87 81
BmtI G_CTAG'C
Cuts at: 320 2507 320
Size: 2187 6806
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1869 4298 5898 7360 1869
Size: 2429 1600 1462 3502
Fragments arranged by size:
3502 2429 1600 1462
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 386 575 1205 1279 1662 2063 2969 3029 3426
Size: 189 630 74 383 401 906 60 397
Cuts at: 3426 3680 3701 4586 5641 386
Size: 254 21 885 1055 3738
Fragments arranged by size:
3738 1055 906 885 630 401 397 383
254 189 74 60 21
Bpu10I CC'TnA_GC
Cuts at: 428 1865 3422 3779 4790 5063 5615 5897 5962
Size: 1437 1557 357 1011 273 552 282 65
Cuts at: 5962 428
Size: 3459
Fragments arranged by size:
3459 1557 1437 1011 552 357 282 273
65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1475 2408 2906 6169 7048 7762 8060 1475
Size: 933 498 3263 879 714 298 2408
Fragments arranged by size:
3263 2408 933 879 714 498 298
BsaI GGTCTCn'nnnn_
Cuts at: 316 2540 2945 3185 4533 4725 8697 316
Size: 2224 405 240 1348 192 3972 612
Fragments arranged by size:
3972 2224 1348 612 405 240 192
BsaAI yAC'GTr
Cuts at: 4383 6346 4383
Size: 1963 7030
BsaBI GATnn'nnATC
Cuts at: 6 6507 8544 6
Size: 6501 2037 455
Fragments arranged by size:
6501 2037 455
BsaHI Gr'CG_yC
Cuts at: 5472 5789 6424 6672 7374 8870 5472
Size: 317 635 248 702 1496 5595
Fragments arranged by size:
5595 1496 702 635 317 248
BsaWI w'CCGG_w
Cuts at: 3239 5249 6182 6703 7381 7792 7939 8213 3239
Size: 2010 933 521 678 411 147 274 4019
Fragments arranged by size:
4019 2010 933 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 492 522 1251 1281 3399 3429 4229 4259 492
Size: 30 729 30 2118 30 800 30 5226
Fragments arranged by size:
5226 2118 800 729 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 302 416 819 822 825 828 831 1259 1538
Size: 114 403 3 3 3 3 428 279
Cuts at: 1538 1608 1770 2099 2102 3406 3882 4218 5416
Size: 70 162 329 3 1304 476 336 1198
Cuts at: 5416 5419 5887 302
Size: 3 468 3408
Fragments arranged by size:
3408 1304 1198 476 468 428 403 336
329 279 162 114 70 3 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 2066 3586 4231 4880 4961 6418 7176 7841 8249
Size: 1520 645 649 81 1457 758 665 408
Cuts at: 8249 2066
Size: 2810
Fragments arranged by size:
2810 1520 1457 758 665 649 645 408
81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 1584 472
Size: 1112 7881
BsiEI CG_ry'CG
Cuts at: 104 6535 6581 7811 104
Size: 6431 46 1230 1286
Fragments arranged by size:
6431 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 603 1482 1732 1800 6007 6785 6975 7835 603
Size: 879 250 68 4207 778 190 860 1761
Fragments arranged by size:
4207 1761 879 860 778 250 190 68
BsmI GAATG_Cn'
Cuts at: 912 3991 912
Size: 3079 5914
BsmBI CGTCTCn'nnnn_
Cuts at: 2809 2809
Size: 8993
Bsp1286I G_dGCh'C
Cuts at: 603 1482 1732 1800 2176 2563 2754 3315 3511
Size: 879 250 68 376 387 191 561 196
Cuts at: 3511 3579 4881 5213 5525 5594 5896 5923 6007
Size: 68 1302 332 312 69 302 27 84
Cuts at: 6007 6618 6711 6785 6975 7037 7835 603
Size: 611 93 74 190 62 798 1761
Fragments arranged by size:
1761 1302 879 798 611 561 387 376
332 312 302 250 196 191 190 93
84 74 69 68 68 62 27
BspHI T'CATG_A
Cuts at: 3135 3863 7425 3135
Size: 728 3562 4703
Fragments arranged by size:
4703 3562 728
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3328 6043 6257 6559 6940 3328
Size: 2715 214 302 381 5381
Fragments arranged by size:
5381 2715 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2882 5480 5982 7285 7339 8823 8988 50
Size: 2832 2598 502 1303 54 1484 165 55
Fragments arranged by size:
2832 2598 1484 1303 502 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2034 3003 5661 6905 2034
Size: 969 2658 1244 4122
Fragments arranged by size:
4122 2658 1244 969
BsrFI r'CCGG_y
Cuts at: 1785 6991 7172 1785
Size: 5206 181 3606
Fragments arranged by size:
5206 3606 181
BsrGI T'GTAC_A
Cuts at: 5047 5047
Size: 8993
BssSI C'ACGA_G
Cuts at: 7264 7972 7264
Size: 708 8285
BstAPI GCAn_nnn'nTGC
Cuts at: 298 301 1092 1795 2373 6370 298
Size: 3 791 703 578 3997 2921
Fragments arranged by size:
3997 2921 791 703 578 3
BstBI TT'CG_AA
Cuts at: 4216 7354 4216
Size: 3138 5855
BstEII G'GTnAC_C
Cuts at: 340 775 2383 5260 340
Size: 435 1608 2877 4073
Fragments arranged by size:
4073 2877 1608 435
BstXI CCAn_nnnn'nTGG
Cuts at: 229 2478 3586 229
Size: 2249 1108 5636
Fragments arranged by size:
5636 2249 1108
BstYI r'GATC_y
Cuts at: 1 913 1455 3675 6018 6485 6508 6843 7089
Size: 912 542 2220 2343 467 23 335 246
Cuts at: 7089 7395 7407 7493 8545 1
Size: 306 12 86 1052 449
Fragments arranged by size:
2343 2220 1052 912 542 467 449 335
306 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1100 1100
Size: 8993
Bsu36I CC'TnA_GG
Cuts at: 1157 4622 1157
Size: 3465 5528
BtgI C'CryG_G
Cuts at: 138 2322 2855 2991 3463 4138 138
Size: 2184 533 136 472 675 4993
Fragments arranged by size:
4993 2184 675 533 472 136
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 325 6943 7124 7287 325
Size: 6618 181 163 2031
Fragments arranged by size:
6618 2031 181 163
BtsI GCAGTG_nn'
Cuts at: 264 270 276 1317 2955 5562 5772 6236 264
Size: 6 6 1041 1638 2607 210 464 3021
Fragments arranged by size:
3021 2607 1638 1041 464 210 6 6
DraI TTT'AAA
Cuts at: 5992 5992
Size: 8993
DraIII CAC_nnn'GTG
Cuts at: 936 1795 936
Size: 859 8134
DrdI GACnn_nn'nnGTC
Cuts at: 6699 8043 6699
Size: 1344 7649
EaeI y'GGCC_r
Cuts at: 330 702 801 1496 1722 2148 2157 2319 5447
Size: 372 99 695 226 426 9 162 3128
Cuts at: 5447 6578 6752 7143 7170 8515 330
Size: 1131 174 391 27 1345 808
Fragments arranged by size:
3128 1345 1131 808 695 426 391 372
226 174 162 99 27 9
EagI C'GGCC_G
Cuts at: 6578 6578
Size: 8993
EarI CTCTTCn'nnn_
Cuts at: 185 1838 3463 4267 4321 4400 4406 4637 5405
Size: 1653 1625 804 54 79 6 231 768
Cuts at: 5405 7016 7226 185
Size: 1611 210 1952
Fragments arranged by size:
1952 1653 1625 1611 804 768 231 210
79 54 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3186 7201 7943 8089 8803 3186
Size: 4015 742 146 714 3376
Fragments arranged by size:
4015 3376 742 714 146
EcoICRI GAG'CTC
Cuts at: 6005 6005
Size: 8993
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 386 575 1182 1205 1279 1631 1649 1660 1662
Size: 189 607 23 74 352 18 11 2
Cuts at: 1662 2063 2362 2969 3029 3426 3680 3701 3962
Size: 401 299 607 60 397 254 21 261
Cuts at: 3962 4307 4391 4586 4661 5585 5641 6818 7250
Size: 345 84 195 75 924 56 1177 432
Cuts at: 7250 7603 8265 386
Size: 353 662 1114
Fragments arranged by size:
1177 1114 924 662 607 607 432 401
397 353 352 345 299 261 254 195
189 84 75 74 60 56 23 21
18 11 2
EcoNI CCTnn'n_nnAGG
Cuts at: 691 1566 2081 2365 2568 3335 4286 691
Size: 875 515 284 203 767 951 5398
Fragments arranged by size:
5398 951 875 767 515 284 203
EcoO109I rG'GnC_Cy
Cuts at: 1031 1172 1571 2173 2511 2559 2560 2742 3078
Size: 141 399 602 338 48 1 182 336
Cuts at: 3078 3327 4062 4877 5179 5500 6132 1031
Size: 249 735 815 302 321 632 3892
Fragments arranged by size:
3892 815 735 632 602 399 338 336
321 302 249 182 141 48 1
EcoRI G'AATT_C
Cuts at: 3345 5997 3345
Size: 2652 6341
EcoRV GAT'ATC
Cuts at: 4029 4029
Size: 8993
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 7012 7012
Size: 8993
FspI TGC'GCA
Cuts at: 6774 6774
Size: 8993
HaeII r_GCGC'y
Cuts at: 1865 3594 3983 5152 5475 5743 5792 6427 6675
Size: 1729 389 1169 323 268 49 635 248
Cuts at: 6675 7905 8676 1865
Size: 1230 771 2182
Fragments arranged by size:
2182 1729 1230 1169 771 635 389 323
268 248 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 492 524 1371 1403 1969 2001 2107 2139 2607
Size: 32 847 32 566 32 106 32 468
Cuts at: 2607 2639 2641 2673 2690 2720 2722 2752 4018
Size: 32 2 32 17 30 2 30 1266
Cuts at: 4018 4050 4249 4281 6835 6865 6867 6897 492
Size: 32 199 32 2554 30 2 30 2588
Fragments arranged by size:
2588 2554 1266 847 566 468 199 106
32 32 32 32 32 32 32 32
30 30 30 30 17 2 2 2
HincII GTy'rAC
Cuts at: 6032 6032
Size: 8993
KasI G'GCGC_C
Cuts at: 5471 5788 6423 6671 5471
Size: 317 635 248 7793
Fragments arranged by size:
7793 635 317 248
KpnI G_GTAC'C
Cuts at: 1438 6013 1438
Size: 4575 4418
MluI A'CGCG_T
Cuts at: 8426 8426
Size: 8993
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1390 1910 2150 2836 5957 7752 7936 1390
Size: 520 240 686 3121 1795 184 2447
Fragments arranged by size:
3121 2447 1795 686 520 240 184
MscI TGG'CCA
Cuts at: 332 704 803 1498 1724 2150 2159 2321 6754
Size: 372 99 695 226 426 9 162 4433
Cuts at: 6754 332
Size: 2571
Fragments arranged by size:
4433 2571 695 426 372 226 162 99
9
MslI CAynn'nnrTG
Cuts at: 2771 3067 3320 5338 5369 6982 7109 2771
Size: 296 253 2018 31 1613 127 4655
Fragments arranged by size:
4655 2018 1613 296 253 127 31
MspA1I CmG'CkG
Cuts at: 298 744 1225 1638 2170 2993 3354 3413 3710
Size: 446 481 413 532 823 361 59 297
Cuts at: 3710 6104 6418 6778 7562 7807 298
Size: 2394 314 360 784 245 1484
Fragments arranged by size:
2394 1484 823 784 532 481 446 413
361 360 314 297 245 59
NaeI GCC'GGC
Cuts at: 7174 7174
Size: 8993
NarI GG'CG_CC
Cuts at: 5472 5789 6424 6672 5472
Size: 317 635 248 7793
Fragments arranged by size:
7793 635 317 248
NcoI C'CATG_G
Cuts at: 2322 2855 2322
Size: 533 8460
NgoMIV G'CCGG_C
Cuts at: 7172 7172
Size: 8993
NheI G'CTAG_C
Cuts at: 316 2503 316
Size: 2187 6806
NsiI A_TGCA'T
Cuts at: 2058 2058
Size: 8993
NspI r_CATG'y
Cuts at: 1090 1527 1875 4459 6046 6546 8149 1090
Size: 437 348 2584 1587 500 1603 1934
Fragments arranged by size:
2584 1934 1603 1587 500 437 348
PasI CC'CwG_GG
Cuts at: 471 2564 2746 3184 4554 471
Size: 2093 182 438 1370 4910
Fragments arranged by size:
4910 2093 1370 438 182
PciI A'CATG_T
Cuts at: 1523 8145 1523
Size: 6622 2371
PflMI CCAn_nnn'nTGG
Cuts at: 4968 4968
Size: 8993
PfoI T'CCnGG_A
Cuts at: 2079 2211 3717 2079
Size: 132 1506 7355
Fragments arranged by size:
7355 1506 132
PmeI GTTT'AAAC
Cuts at: 5992 5992
Size: 8993
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3399 3431 7439 7471 3399
Size: 32 4008 32 4921
Fragments arranged by size:
4921 4008 32 32
PpuMI rG'GwC_Cy
Cuts at: 1031 1172 2742 3078 3327 4062 5179 1031
Size: 141 1570 336 249 735 1117 4845
Fragments arranged by size:
4845 1570 1117 735 336 249 141
PsiI TTA'TAA
Cuts at: 3362 3362
Size: 8993
PspOMI G'GGCC_C
Cuts at: 2172 2559 4877 2172
Size: 387 2318 6288
Fragments arranged by size:
6288 2318 387
PstI C_TGCA'G
Cuts at: 1407 2007 2169 2369 3441 4821 5177 5735 6040
Size: 600 162 200 1072 1380 356 558 305
Cuts at: 6040 6725 1407
Size: 685 3675
Fragments arranged by size:
3675 1380 1072 685 600 558 356 305
200 162
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8993
PvuII CAG'CTG
Cuts at: 298 1225 1638 3354 6418 6778 298
Size: 927 413 1716 3064 360 2513
Fragments arranged by size:
3064 2513 1716 927 413 360
RsrII CG'GwC_CG
Cuts at: 7188 7188
Size: 8993
SacI G_AGCT'C
Cuts at: 6007 6007
Size: 8993
SacII CC_GC'GG
Cuts at: 2994 2994
Size: 8993
SalI G'TCGA_C
Cuts at: 6030 6030
Size: 8993
SanDI GG'GwC_CC
Cuts at: 1031 2742 3078 3327 1031
Size: 1711 336 249 6697
Fragments arranged by size:
6697 1711 336 249
SapI GCTCTTCn'nnn_
Cuts at: 5405 7016 7226 5405
Size: 1611 210 7172
Fragments arranged by size:
7172 1611 210
SbfI CC_TGCA'GG
Cuts at: 2369 5735 6040 2369
Size: 3366 305 5322
Fragments arranged by size:
5322 3366 305
ScaI AGT'ACT
Cuts at: 1488 2421 8510 1488
Size: 933 6089 1971
Fragments arranged by size:
6089 1971 933
SexAI A'CCwGG_T
Cuts at: 3801 5213 5705 3801
Size: 1412 492 7089
Fragments arranged by size:
7089 1412 492
SfcI C'TryA_G
Cuts at: 33 407 730 1251 1403 2003 2165 2226 2291
Size: 374 323 521 152 600 162 61 65
Cuts at: 2291 2365 3437 4169 4817 5173 5731 6036 6721
Size: 74 1072 732 648 356 558 305 685
Cuts at: 6721 7689 7880 33
Size: 968 191 1146
Fragments arranged by size:
1146 1072 968 732 685 648 600 558
521 374 356 323 305 191 162 152
74 65 61
SfiI GGCCn_nnn'nGGCC
Cuts at: 444 2156 444
Size: 1712 7281
SfoI GGC'GCC
Cuts at: 5473 5790 6425 6673 5473
Size: 317 635 248 7793
Fragments arranged by size:
7793 635 317 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8993
SmaI CCC'GGG
Cuts at: 1936 6015 1936
Size: 4079 4914
SmlI C'TyrA_G
Cuts at: 1490 2423 2921 6148 7063 7777 8039 1490
Size: 933 498 3227 915 714 262 2444
Fragments arranged by size:
3227 2444 933 915 714 498 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8993
SphI G_CATG'C
Cuts at: 6046 6546 6046
Size: 500 8493
SspI AAT'ATT
Cuts at: 4197 4197
Size: 8993
StuI AGG'CCT
Cuts at: 438 438
Size: 8993
StyI C'CwwG_G
Cuts at: 543 2322 2855 3475 4058 4399 4533 4634 5795
Size: 1779 533 620 583 341 134 101 1161
Cuts at: 5795 6127 6481 543
Size: 332 354 3055
Fragments arranged by size:
3055 1779 1161 620 583 533 354 341
332 134 101
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2817 3107 4546 4975 5248 5495 6576 6709 7168
Size: 290 1439 429 273 247 1081 133 459
Cuts at: 7168 7377 2817
Size: 209 4433
Fragments arranged by size:
4433 1439 1081 459 429 290 273 247
209 133
TatI w'GTAC_w
Cuts at: 1145 1486 2419 5047 8508 1145
Size: 341 933 2628 3461 1630
Fragments arranged by size:
3461 2628 1630 933 341
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 163 195 920 952 5171 5203 5244 5276 8510
Size: 32 725 32 4219 32 41 32 3234
Cuts at: 8510 8542 163
Size: 32 614
Fragments arranged by size:
4219 3234 725 614 41 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 4354 5193 6790 4354
Size: 839 1597 6557
Fragments arranged by size:
6557 1597 839
XbaI T'CTAG_A
Cuts at: 6024 6024
Size: 8993
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3760 4965 6488 3760
Size: 1205 1523 6265
Fragments arranged by size:
6265 1523 1205
XmaI C'CCGG_G
Cuts at: 1934 6013 1934
Size: 4079 4914
XmnI GAAnn'nnTTC
Cuts at: 438 2375 438
Size: 1937 7056
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AgeI AscI AvrII BmgBI BsiWI
BspEI BssHII ClaI CspCI FseI FspAI HindIII HpaI
MfeI NdeI NotI NruI PacI PmlI PshAI PspXI
PsrI SgrAI SnaBI SrfI SwaI XhoI ZraI