Restriction map for
pF1KA0294
(Circular) (Six-base) MAPSORT of: pF1KA0294.seq Check: 3305 from: 1 to: 7145
pF1KA0294 7145 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3467 3467
Size: 7145
AccI GT'mk_AC
Cuts at: 1322 3249 4183 1322
Size: 1927 934 4284
Fragments arranged by size:
4284 1927 934
Acc65I G'GTAC_C
Cuts at: 4161 4161
Size: 7145
AclI AA'CG_TT
Cuts at: 6985 6985
Size: 7145
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1084 1444 1786 1789 2140 3058 3982 4970 5402
Size: 360 342 3 351 918 924 988 432
Cuts at: 5402 6149 6417 1084
Size: 747 268 1812
Fragments arranged by size:
1812 988 924 918 747 432 360 351
342 268 3
AfeI AGC'GCT
Cuts at: 441 441
Size: 7145
AflII C'TTAA_G
Cuts at: 1351 1351
Size: 7145
AflIII A'CryG_T
Cuts at: 1627 2142 3266 4497 5601 6578 1627
Size: 515 1124 1231 1104 977 2194
Fragments arranged by size:
2194 1231 1124 1104 977 515
AgeI A'CCGG_T
Cuts at: 5533 5533
Size: 7145
AleI CACnn'nnGTG
Cuts at: 334 430 1606 334
Size: 96 1176 5873
Fragments arranged by size:
5873 1176 96
AlwNI CAG_nnn'CTG
Cuts at: 778 3135 3516 4450 6017 778
Size: 2357 381 934 1567 1906
Fragments arranged by size:
2357 1906 1567 934 381
ApaLI G'TGCA_C
Cuts at: 4060 5915 4060
Size: 1855 5290
ApoI r'AATT_y
Cuts at: 865 1212 1654 2462 2856 3670 4149 6498 6754
Size: 347 442 808 394 814 479 2349 256
Cuts at: 6754 865
Size: 1256
Fragments arranged by size:
2349 1256 814 808 479 442 394 347
256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7145
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7145
AvaI C'yCGr_G
Cuts at: 1267 2479 2965 3742 4165 1267
Size: 1212 486 777 423 4247
Fragments arranged by size:
4247 1212 777 486 423
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2263 2296 2263
Size: 33 7112
BamHI G'GATC_C
Cuts at: 3190 3966 4170 3190
Size: 776 204 6165
Fragments arranged by size:
6165 776 204
BanI G'GyrC_C
Cuts at: 486 510 1099 2195 2952 3506 3562 4098 4161
Size: 24 589 1096 757 554 56 536 63
Cuts at: 4161 4575 4823 4858 486
Size: 414 248 35 2773
Fragments arranged by size:
2773 1096 757 589 554 536 414 248
63 56 35 24
BanII G_rGCy'C
Cuts at: 125 1826 3183 4159 5189 125
Size: 1701 1357 976 1030 2081
Fragments arranged by size:
2081 1701 1357 1030 976
BbeI G_GCGC'C
Cuts at: 4102 4579 4827 4102
Size: 477 248 6420
Fragments arranged by size:
6420 477 248
BbsI GAAGACnn'nnnn_
Cuts at: 1432 1527 2022 2636 3269 3288 3764 1432
Size: 95 495 614 633 19 476 4813
Fragments arranged by size:
4813 633 614 495 476 95 19
BbvCI CC'TCA_GC
Cuts at: 443 443
Size: 7145
BciVI GTATCCnnnnn_n'
Cuts at: 5043 5804 7145 5043
Size: 761 1341 5043
Fragments arranged by size:
5043 1341 761
BclI T'GATC_A
Cuts at: 1869 3218 4665 1869
Size: 1349 1447 4349
Fragments arranged by size:
4349 1447 1349
BfrBI ATG'CAT
Cuts at: 1573 1573
Size: 7145
BglI GCCn_nnn'nGGC
Cuts at: 694 4021 694
Size: 3327 3818
BglII A'GATC_T
Cuts at: 1189 4660 6697 1189
Size: 3471 2037 1637
Fragments arranged by size:
3471 2037 1637
BlpI GC'TnA_GC
Cuts at: 4257 4257
Size: 7145
Bme1580I G_kGCm'C
Cuts at: 437 473 515 2955 3509 4064 4770 4863 5919
Size: 36 42 2440 554 555 706 93 1056
Cuts at: 5919 437
Size: 1663
Fragments arranged by size:
2440 1663 1056 706 555 554 93 42
36
BmgBI CAC'GTC
Cuts at: 572 3125 3601 572
Size: 2553 476 4116
Fragments arranged by size:
4116 2553 476
BmrI ACTGGGnnnn_n'
Cuts at: 328 1318 2146 4357 4438 4773 6917 328
Size: 990 828 2211 81 335 2144 556
Fragments arranged by size:
2211 2144 990 828 556 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1527 3920 5512 1527
Size: 2393 1592 3160
Fragments arranged by size:
3160 2393 1592
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 338 531 1135 1738 3358 3897 293
Size: 45 193 604 603 1620 539 3541
Fragments arranged by size:
3541 1620 604 603 539 193 45
Bpu10I CC'TnA_GC
Cuts at: 443 1045 3895 443
Size: 602 2850 3693
Fragments arranged by size:
3693 2850 602
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1219 1603 3436 4321 5200 5692 5990 1219
Size: 384 1833 885 879 492 298 2374
Fragments arranged by size:
2374 1833 885 879 492 384 298
BsaI GGTCTCn'nnnn_
Cuts at: 466 2962 3645 6849 466
Size: 2496 683 3204 762
Fragments arranged by size:
3204 2496 762 683
BsaAI yAC'GTr
Cuts at: 1341 4498 1341
Size: 3157 3988
BsaBI GATnn'nnATC
Cuts at: 6 1868 2639 2839 4659 6696 6
Size: 1862 771 200 1820 2037 455
Fragments arranged by size:
2037 1862 1820 771 455 200
BsaHI Gr'CG_yC
Cuts at: 2150 3230 3389 4099 4576 4824 5526 7022 2150
Size: 1080 159 710 477 248 702 1496 2273
Fragments arranged by size:
2273 1496 1080 710 702 477 248 159
BsaWI w'CCGG_w
Cuts at: 3283 3625 4334 4855 5533 5807 5954 6365 3283
Size: 342 709 521 678 274 147 411 4063
Fragments arranged by size:
4063 709 678 521 411 342 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 846 876 1991 2021 3278 3308 3566 3596 3862
Size: 30 1115 30 1257 30 258 30 266
Cuts at: 3862 3892 846
Size: 30 4099
Fragments arranged by size:
4099 1257 1115 266 258 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 460 463 652 2012 2614 2659 2944 3034 3800
Size: 3 189 1360 602 45 285 90 766
Cuts at: 3800 3870 460
Size: 70 3735
Fragments arranged by size:
3735 1360 766 602 285 189 90 70
45 3
BseYI C'CCAG_C
Cuts at: 436 692 3846 4570 5328 5905 6401 436
Size: 256 3154 724 758 577 496 1180
Fragments arranged by size:
3154 1180 758 724 577 496 256
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 275 3679 275
Size: 3404 3741
BsiEI CG_ry'CG
Cuts at: 104 669 1689 2506 2978 4106 4687 4733 5941
Size: 565 1020 817 472 1128 581 46 1208
Cuts at: 5941 104
Size: 1308
Fragments arranged by size:
1308 1208 1128 1020 817 581 565 472
46
BsiHKAI G_wGCw'C
Cuts at: 362 983 1826 2189 3929 4064 4159 4937 5127
Size: 621 843 363 1740 135 95 778 190
Cuts at: 5127 5919 362
Size: 792 1588
Fragments arranged by size:
1740 1588 843 792 778 621 363 190
135 95
BsmBI CGTCTCn'nnnn_
Cuts at: 2850 3131 2850
Size: 281 6864
Bsp1286I G_dGCh'C
Cuts at: 125 362 437 473 515 983 1826 2189 2955
Size: 237 75 36 42 468 843 363 766
Cuts at: 2955 3183 3509 3929 4064 4159 4770 4863 4937
Size: 228 326 420 135 95 611 93 74
Cuts at: 4937 5127 5189 5919 125
Size: 190 62 730 1351
Fragments arranged by size:
1351 843 766 730 611 468 420 363
326 237 228 190 135 95 93 75
74 62 42 36
BspEI T'CCGG_A
Cuts at: 3283 6365 3283
Size: 3082 4063
BspHI T'CATG_A
Cuts at: 957 2082 3107 3254 6321 957
Size: 1125 1025 147 3067 1781
Fragments arranged by size:
3067 1781 1125 1025 147
BspMI ACCTGCnnnn'nnnn_
Cuts at: 530 1278 3467 4195 4409 4711 5092 530
Size: 748 2189 728 214 302 381 2583
Fragments arranged by size:
2583 2189 748 728 381 302 214
BsrBI CCG'CTC
Cuts at: 50 136 1148 5437 5491 6975 7140 50
Size: 86 1012 4289 54 1484 165 55
Fragments arranged by size:
4289 1484 1012 165 86 55 54
BsrDI GCAATG_nn'
Cuts at: 5057 5057
Size: 7145
BsrFI r'CCGG_y
Cuts at: 3591 5143 5324 5533 3591
Size: 1552 181 209 5203
Fragments arranged by size:
5203 1552 209 181
BssSI C'ACGA_G
Cuts at: 1078 3580 3697 5416 5774 1078
Size: 2502 117 1719 358 2449
Fragments arranged by size:
2502 2449 1719 358 117
BstAPI GCAn_nnn'nTGC
Cuts at: 2906 4522 2906
Size: 1616 5529
BstBI TT'CG_AA
Cuts at: 5506 5506
Size: 7145
BstEII G'GTnAC_C
Cuts at: 3811 3811
Size: 7145
BstXI CCAn_nnnn'nTGG
Cuts at: 336 576 1566 3834 336
Size: 240 990 2268 3647
Fragments arranged by size:
3647 2268 990 240
BstYI r'GATC_y
Cuts at: 1 1189 1451 3190 3395 3966 4121 4170 4637
Size: 1188 262 1739 205 571 155 49 467
Cuts at: 4637 4660 4995 5241 6253 6339 6351 6697 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1739 1188 1012 571 467 449 346 335
262 246 205 155 86 49 23 12
Bsu36I CC'TnA_GG
Cuts at: 3414 3749 3414
Size: 335 6810
BtgI C'CryG_G
Cuts at: 107 1514 1559 1607 2228 2783 3543 3686 107
Size: 1407 45 48 621 555 760 143 3566
Fragments arranged by size:
3566 1407 760 621 555 143 48 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1158 1544 3459 5095 5276 5439 1158
Size: 386 1915 1636 181 163 2864
Fragments arranged by size:
2864 1915 1636 386 181 163
BtsI GCAGTG_nn'
Cuts at: 1465 1944 4388 1465
Size: 479 2444 4222
Fragments arranged by size:
4222 2444 479
ClaI AT'CG_AT
Cuts at: 3853 3853
Size: 7145
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1001 1036 1001
Size: 35 7110
DraI TTT'AAA
Cuts at: 974 1661 4144 974
Size: 687 2483 3975
Fragments arranged by size:
3975 2483 687
DraIII CAC_nnn'GTG
Cuts at: 3272 3272
Size: 7145
DrdI GACnn_nn'nnGTC
Cuts at: 4851 5709 4851
Size: 858 6287
EaeI y'GGCC_r
Cuts at: 707 1487 2225 2322 2579 2696 2975 3377 3683
Size: 780 738 97 257 117 279 402 306
Cuts at: 3683 4730 4904 5295 5322 6667 707
Size: 1047 174 391 27 1345 1185
Fragments arranged by size:
1345 1185 1047 780 738 402 391 306
279 257 174 117 97 27
EagI C'GGCC_G
Cuts at: 2975 4730 2975
Size: 1755 5390
EarI CTCTTCn'nnn_
Cuts at: 173 179 875 1132 1808 2591 3624 4085 5168
Size: 6 696 257 676 783 1033 461 1083
Cuts at: 5168 5378 173
Size: 210 1940
Fragments arranged by size:
1940 1083 1033 783 696 676 461 257
210 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 240 3418 5353 5663 5809 6955 240
Size: 3178 1935 310 146 1146 430
Fragments arranged by size:
3178 1935 1146 430 310 146
EcoICRI GAG'CTC
Cuts at: 1824 4157 1824
Size: 2333 4812
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 338 531 1084 1135 1444 1738 1786 1789
Size: 45 193 553 51 309 294 48 3
Cuts at: 1789 2140 3058 3358 3897 3982 4970 5402 6149
Size: 351 918 300 539 85 988 432 747
Cuts at: 6149 6417 293
Size: 268 1021
Fragments arranged by size:
1021 988 918 747 553 539 432 351
309 300 294 268 193 85 51 48
45 3
EcoNI CCTnn'n_nnAGG
Cuts at: 260 1155 1753 2596 260
Size: 895 598 843 4809
Fragments arranged by size:
4809 895 843 598
EcoO109I rG'GnC_Cy
Cuts at: 243 920 3890 4284 243
Size: 677 2970 394 3104
Fragments arranged by size:
3104 2970 677 394
EcoRI G'AATT_C
Cuts at: 2462 4149 2462
Size: 1687 5458
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3088 5164 3088
Size: 2076 5069
FspI TGC'GCA
Cuts at: 931 4926 931
Size: 3995 3150
HaeII r_GCGC'y
Cuts at: 443 4027 4102 4579 4827 5849 6828 443
Size: 3584 75 477 248 1022 979 760
Fragments arranged by size:
3584 1022 979 760 477 248 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 206 238 714 746 1152 1174 1184 1206 1261
Size: 32 476 32 406 22 10 22 55
Cuts at: 1261 1293 2383 2415 3278 3310 3623 3655 3862
Size: 32 1090 32 863 32 313 32 207
Cuts at: 3862 3894 3916 3948 3958 3990 4987 5017 5019
Size: 32 22 32 10 32 997 30 2
Cuts at: 5019 5049 206
Size: 30 2302
Fragments arranged by size:
2302 1090 997 863 476 406 313 207
55 32 32 32 32 32 32 32
32 32 30 30 22 22 22 10
10 2
HincII GTy'rAC
Cuts at: 375 1230 1323 2565 3153 4184 375
Size: 855 93 1242 588 1031 3336
Fragments arranged by size:
3336 1242 1031 855 588 93
HindIII A'AGCT_T
Cuts at: 1927 1927
Size: 7145
KasI G'GCGC_C
Cuts at: 4098 4575 4823 4098
Size: 477 248 6420
Fragments arranged by size:
6420 477 248
KpnI G_GTAC'C
Cuts at: 4165 4165
Size: 7145
MluI A'CGCG_T
Cuts at: 6578 6578
Size: 7145
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 542 670 2655 5816 6000 542
Size: 128 1985 3161 184 1687
Fragments arranged by size:
3161 1985 1687 184 128
MscI TGG'CCA
Cuts at: 1489 2324 2581 2698 3685 4906 1489
Size: 835 257 117 987 1221 3728
Fragments arranged by size:
3728 1221 987 835 257 117
MslI CAynn'nnrTG
Cuts at: 334 430 1606 2686 3106 3307 3379 5134 5261
Size: 96 1176 1080 420 201 72 1755 127
Cuts at: 5261 334
Size: 2218
Fragments arranged by size:
2218 1755 1176 1080 420 201 127 96
72
MspA1I CmG'CkG
Cuts at: 692 1383 2337 2521 3336 3513 4256 4570 4930
Size: 691 954 184 815 177 743 314 360
Cuts at: 4930 5943 6188 692
Size: 1013 245 1649
Fragments arranged by size:
1649 1013 954 815 743 691 360 314
245 184 177
NaeI GCC'GGC
Cuts at: 5326 5326
Size: 7145
NarI GG'CG_CC
Cuts at: 4099 4576 4824 4099
Size: 477 248 6420
Fragments arranged by size:
6420 477 248
NcoI C'CATG_G
Cuts at: 107 1559 107
Size: 1452 5693
NgoMIV G'CCGG_C
Cuts at: 5324 5324
Size: 7145
NsiI A_TGCA'T
Cuts at: 1575 1575
Size: 7145
NspI r_CATG'y
Cuts at: 1631 1764 2146 2685 2913 3498 4198 4698 5605
Size: 133 382 539 228 585 700 500 907
Cuts at: 5605 1631
Size: 3171
Fragments arranged by size:
3171 907 700 585 539 500 382 228
133
PciI A'CATG_T
Cuts at: 1627 2142 5601 1627
Size: 515 3459 3171
Fragments arranged by size:
3459 3171 515
PflMI CCAn_nnn'nTGG
Cuts at: 2826 2826
Size: 7145
PfoI T'CCnGG_A
Cuts at: 407 1754 407
Size: 1347 5798
PmeI GTTT'AAAC
Cuts at: 4144 4144
Size: 7145
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6284 6316 6284
Size: 32 7113
PpuMI rG'GwC_Cy
Cuts at: 920 3890 920
Size: 2970 4175
PsiI TTA'TAA
Cuts at: 2735 3041 2735
Size: 306 6839
PstI C_TGCA'G
Cuts at: 144 1460 2461 3464 4192 4877 144
Size: 1316 1001 1003 728 685 2412
Fragments arranged by size:
2412 1316 1003 1001 728 685
PvuI CG_AT'CG
Cuts at: 104 1689 104
Size: 1585 5560
PvuII CAG'CTG
Cuts at: 2337 2521 3336 4570 4930 2337
Size: 184 815 1234 360 4552
Fragments arranged by size:
4552 1234 815 360 184
RsrII CG'GwC_CG
Cuts at: 5340 5340
Size: 7145
SacI G_AGCT'C
Cuts at: 1826 4159 1826
Size: 2333 4812
SalI G'TCGA_C
Cuts at: 1321 4182 1321
Size: 2861 4284
SanDI GG'GwC_CC
Cuts at: 3890 3890
Size: 7145
SapI GCTCTTCn'nnn_
Cuts at: 875 4085 5168 5378 875
Size: 3210 1083 210 2642
Fragments arranged by size:
3210 2642 1083 210
SbfI CC_TGCA'GG
Cuts at: 3464 4192 3464
Size: 728 6417
ScaI AGT'ACT
Cuts at: 6662 6662
Size: 7145
SfcI C'TryA_G
Cuts at: 33 140 1456 1701 2457 3323 3460 4188 4873
Size: 107 1316 245 756 866 137 728 685
Cuts at: 4873 5866 6057 33
Size: 993 191 1121
Fragments arranged by size:
1316 1121 993 866 756 728 685 245
191 137 107
SfoI GGC'GCC
Cuts at: 4100 4577 4825 4100
Size: 477 248 6420
Fragments arranged by size:
6420 477 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7145
SmaI CCC'GGG
Cuts at: 2481 4167 2481
Size: 1686 5459
SmlI C'TyrA_G
Cuts at: 1198 1351 1618 3451 4300 5215 5707 5969 1198
Size: 153 267 1833 849 915 492 262 2374
Fragments arranged by size:
2374 1833 915 849 492 267 262 153
SnaBI TAC'GTA
Cuts at: 1341 1341
Size: 7145
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7145
SphI G_CATG'C
Cuts at: 2913 4198 4698 2913
Size: 1285 500 5360
Fragments arranged by size:
5360 1285 500
StyI C'CwwG_G
Cuts at: 107 760 839 1103 1559 4010 4279 4633 107
Size: 653 79 264 456 2451 269 354 2619
Fragments arranged by size:
2619 2451 653 456 354 269 264 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 309 655 2520 2833 4728 4861 5320 5529 309
Size: 346 1865 313 1895 133 459 209 1925
Fragments arranged by size:
1925 1895 1865 459 346 313 209 133
TatI w'GTAC_w
Cuts at: 6660 6660
Size: 7145
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 846 878 1727 1759 2780 2812 3566 3598 6662
Size: 32 849 32 1021 32 754 32 3064
Cuts at: 6662 6694 846
Size: 32 1297
Fragments arranged by size:
3064 1297 1021 849 754 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1164 4942 1164
Size: 3778 3367
XbaI T'CTAG_A
Cuts at: 3921 4176 3921
Size: 255 6890
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1110 3191 3726 4023 4640 1110
Size: 2081 535 297 617 3615
Fragments arranged by size:
3615 2081 617 535 297
XmaI C'CCGG_G
Cuts at: 2479 4165 2479
Size: 1686 5459
XmnI GAAnn'nnTTC
Cuts at: 1216 1594 3030 1216
Size: 378 1436 5331
Fragments arranged by size:
5331 1436 378
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PflMI PfoI PmeI PpiI PpuMI
PsiI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AhdI AlfI AloI ApaI AscI AvrII BcgI
BmtI BsiWI BsmI BsrGI BssHII BstZ17I EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI PacI
PasI PmlI PshAI PspOMI PspXI PsrI SacII SexAI
SfiI SgrAI SrfI SspI StuI SwaI XhoI ZraI