Restriction map for
pF1KA0302
(Circular) (Six-base) MAPSORT of: pF1KA0302.seq Check: 123 from: 1 to: 10283
pF1KA0302 10283 bp
With 182 enzymes: SgfI *
August 5, 2008 16:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5670 5670
Size: 10283
AccI GT'mk_AC
Cuts at: 1256 6242 6703 7321 1256
Size: 4986 461 618 4218
Fragments arranged by size:
4986 4218 618 461
Acc65I G'GTAC_C
Cuts at: 7299 7299
Size: 10283
AclI AA'CG_TT
Cuts at: 10123 10123
Size: 10283
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 811 2218 2884 3398 3848 3997 5164 5767 6118
Size: 1407 666 514 450 149 1167 603 351
Cuts at: 6118 7139 7239 8108 8540 9287 9555 811
Size: 1021 100 869 432 747 268 1539
Fragments arranged by size:
1539 1407 1167 1021 869 747 666 603
514 450 432 351 268 149 100
AfeI AGC'GCT
Cuts at: 1591 5521 6222 1591
Size: 3930 701 5652
Fragments arranged by size:
5652 3930 701
AflIII A'CryG_T
Cuts at: 4016 6842 6987 7635 8739 9716 4016
Size: 2826 145 648 1104 977 4583
Fragments arranged by size:
4583 2826 1104 977 648 145
AgeI A'CCGG_T
Cuts at: 2128 8671 2128
Size: 6543 3740
AleI CACnn'nnGTG
Cuts at: 1331 2836 1331
Size: 1505 8778
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4681 5915 4681
Size: 1234 9049
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4498 4530 5219 5251 4498
Size: 32 689 32 9530
Fragments arranged by size:
9530 689 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1725 1782 2334 2862 3546 4199 4389 4704 5004
Size: 57 552 528 684 653 190 315 300
Cuts at: 5004 5496 5814 6463 6561 6833 7588 9155 1725
Size: 492 318 649 98 272 755 1567 2853
Fragments arranged by size:
2853 1567 755 684 653 649 552 528
492 318 315 300 272 190 98 57
ApaI G_GGCC'C
Cuts at: 2013 2195 2481 3459 5778 6641 6673 6951 2013
Size: 182 286 978 2319 863 32 278 5345
Fragments arranged by size:
5345 2319 978 863 286 278 182 32
ApaLI G'TGCA_C
Cuts at: 499 3006 9053 499
Size: 2507 6047 1729
Fragments arranged by size:
6047 2507 1729
ApoI r'AATT_y
Cuts at: 5311 7287 9636 9892 5311
Size: 1976 2349 256 5702
Fragments arranged by size:
5702 2349 1976 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 10283
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10283
AvaI C'yCGr_G
Cuts at: 323 391 402 2425 2569 2624 3146 3164 3503
Size: 68 11 2023 144 55 522 18 339
Cuts at: 3503 4494 5327 5915 5981 6641 6665 6737 7145
Size: 991 833 588 66 660 24 72 408
Cuts at: 7145 7157 7303 323
Size: 12 146 3303
Fragments arranged by size:
3303 2023 991 833 660 588 522 408
339 146 144 72 68 66 55 24
18 12 11
AvrII C'CTAG_G
Cuts at: 3066 3066
Size: 10283
BamHI G'GATC_C
Cuts at: 443 870 3788 5234 7308 443
Size: 427 2918 1446 2074 3418
Fragments arranged by size:
3418 2918 2074 1446 427
BanI G'GyrC_C
Cuts at: 662 1790 2131 2586 4553 6089 7131 7299 7713
Size: 1128 341 455 1967 1536 1042 168 414
Cuts at: 7713 7961 7996 662
Size: 248 35 2949
Fragments arranged by size:
2949 1967 1536 1128 1042 455 414 341
248 168 35
BanII G_rGCy'C
Cuts at: 520 1358 2013 2195 2481 3459 3486 3800 4136
Size: 838 655 182 286 978 27 314 336
Cuts at: 4136 4733 4878 5390 5778 6551 6641 6673 6951
Size: 597 145 512 388 773 90 32 278
Cuts at: 6951 7060 7297 8327 520
Size: 109 237 1030 2476
Fragments arranged by size:
2476 1030 978 838 773 655 597 512
388 336 314 286 278 237 182 145
109 90 32 27
BbeI G_GCGC'C
Cuts at: 4557 6093 7717 7965 4557
Size: 1536 1624 248 6875
Fragments arranged by size:
6875 1624 1536 248
BbsI GAAGACnn'nnnn_
Cuts at: 623 679 884 3043 3919 6984 7091 623
Size: 56 205 2159 876 3065 107 3815
Fragments arranged by size:
3815 3065 2159 876 205 107 56
BbvCI CC'TCA_GC
Cuts at: 382 2066 2103 2973 3659 5662 5732 7196 382
Size: 1684 37 870 686 2003 70 1464 3469
Fragments arranged by size:
3469 2003 1684 1464 870 686 70 37
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2305 2339 2917 2951 4069 4103 4549 4583 2305
Size: 34 578 34 1118 34 446 34 8005
Fragments arranged by size:
8005 1118 578 446 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8181 8942 10283 8181
Size: 761 1341 8181
Fragments arranged by size:
8181 1341 761
BclI T'GATC_A
Cuts at: 7803 7803
Size: 10283
BfrBI ATG'CAT
Cuts at: 826 826
Size: 10283
BglI GCCn_nnn'nGGC
Cuts at: 1012 1855 2653 3418 4084 4903 5422 1012
Size: 843 798 765 666 819 519 5873
Fragments arranged by size:
5873 843 819 798 765 666 519
BglII A'GATC_T
Cuts at: 6056 7798 9835 6056
Size: 1742 2037 6504
Fragments arranged by size:
6504 2037 1742
BlpI GC'TnA_GC
Cuts at: 1429 2680 3670 4262 4799 6224 7395 1429
Size: 1251 990 592 537 1425 1171 4317
Fragments arranged by size:
4317 1425 1251 1171 990 592 537
Bme1580I G_kGCm'C
Cuts at: 503 2013 2136 2195 2328 2438 2481 2591 3010
Size: 1510 123 59 133 110 43 110 419
Cuts at: 3010 3459 4290 4977 5538 5778 6641 6673 6951
Size: 449 831 687 561 240 863 32 278
Cuts at: 6951 7136 7908 8001 9057 503
Size: 185 772 93 1056 1729
Fragments arranged by size:
1729 1510 1056 863 831 772 687 561
449 419 278 240 185 133 123 110
110 93 59 43 32
BmgBI CAC'GTC
Cuts at: 882 4017 882
Size: 3135 7148
BmrI ACTGGGnnnn_n'
Cuts at: 213 859 2283 3516 6512 7171 7495 7576 7911
Size: 646 1424 1233 2996 659 324 81 335
Cuts at: 7911 10055 213
Size: 2144 441
Fragments arranged by size:
2996 2144 1424 1233 659 646 441 335
324 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 417 4509 6075 7074 8650 417
Size: 4092 1566 999 1576 2050
Fragments arranged by size:
4092 2050 1576 1566 999
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 475 741 1333 1396 1492 1720 2038 2230 2503
Size: 266 592 63 96 228 318 192 273
Cuts at: 2503 2617 2779 2788 2818 3151 3568 3703 3769
Size: 114 162 9 30 333 417 135 66
Cuts at: 3769 3823 4231 4312 4324 4879 5026 5177 5689
Size: 54 408 81 12 555 147 151 512
Cuts at: 5689 5741 6343 6416 7130 475
Size: 52 602 73 714 3628
Fragments arranged by size:
3628 714 602 592 555 512 417 408
333 318 273 266 228 192 162 151
147 135 114 96 81 73 66 63
54 52 30 12 9
Bpu10I CC'TnA_GC
Cuts at: 382 1117 2066 2103 2973 2980 3200 3659 5662
Size: 735 949 37 870 7 220 459 2003
Cuts at: 5662 5732 6618 7196 382
Size: 70 886 578 3469
Fragments arranged by size:
3469 2003 949 886 870 735 578 459
220 70 37 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 2544 4162 4357 5488 6601 7459 8338 8830
Size: 2075 1618 195 1131 1113 858 879 492
Cuts at: 8830 9128 469
Size: 298 1624
Fragments arranged by size:
2075 1624 1618 1131 1113 879 858 492
298 195
BsaI GGTCTCn'nnnn_
Cuts at: 248 1414 2114 2785 3007 4178 4207 9987 248
Size: 1166 700 671 222 1171 29 5780 544
Fragments arranged by size:
5780 1171 1166 700 671 544 222 29
BsaAI yAC'GTr
Cuts at: 332 7636 332
Size: 7304 2979
BsaBI GATnn'nnATC
Cuts at: 6 5104 7797 9834 6
Size: 5098 2693 2037 455
Fragments arranged by size:
5098 2693 2037 455
BsaHI Gr'CG_yC
Cuts at: 1562 2519 2940 3273 3773 4554 6090 7714 7962
Size: 957 421 333 500 781 1536 1624 248
Cuts at: 7962 8664 10160 1562
Size: 702 1496 1685
Fragments arranged by size:
1685 1624 1536 1496 957 781 702 500
421 333 248
BsaWI w'CCGG_w
Cuts at: 2128 5580 7472 7993 8671 8945 9092 9503 2128
Size: 3452 1892 521 678 274 147 411 2908
Fragments arranged by size:
3452 2908 1892 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 502 532 619 649 2377 2407 2431 2461 4498
Size: 30 87 30 1728 30 24 30 2037
Cuts at: 4498 4528 5980 6010 502
Size: 30 1452 30 4775
Fragments arranged by size:
4775 2037 1728 1452 87 30 30 30
30 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 379 1678 1681 2332 2500 2503 2626 2728 3508
Size: 1299 3 651 168 3 123 102 780
Cuts at: 3508 3586 3894 4522 5749 5988 6067 6319 6358
Size: 78 308 628 1227 239 79 252 39
Cuts at: 6358 6391 6394 6397 379
Size: 33 3 3 4265
Fragments arranged by size:
4265 1299 1227 780 651 628 308 252
239 168 123 102 79 78 39 33
3 3 3 3
BseYI C'CCAG_C
Cuts at: 182 2187 2327 2430 3082 3245 3288 3566 3576
Size: 2005 140 103 652 163 43 278 10
Cuts at: 3576 4199 4319 4669 4877 5271 5395 5777 6228
Size: 623 120 350 208 394 124 382 451
Cuts at: 6228 6485 6564 6999 7135 7708 8466 9043 9539
Size: 257 79 435 136 573 758 577 496
Cuts at: 9539 182
Size: 926
Fragments arranged by size:
2005 926 758 652 623 577 573 496
451 435 394 382 350 278 257 208
163 140 136 124 120 103 79 43
10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 1570 1807 1873 2291 2596 2797 3256 4174
Size: 1269 237 66 418 305 201 459 918
Cuts at: 4174 4309 4493 4793 5393 5512 5578 5704 6519
Size: 135 184 300 600 119 66 126 815
Cuts at: 6519 301
Size: 4065
Fragments arranged by size:
4065 1269 918 815 600 459 418 305
300 237 201 184 135 126 119 66
66
BsiEI CG_ry'CG
Cuts at: 104 1082 1401 1545 2184 5745 6050 6242 7825
Size: 978 319 144 639 3561 305 192 1583
Cuts at: 7825 7871 9079 104
Size: 46 1208 1308
Fragments arranged by size:
3561 1583 1308 1208 978 639 319 305
192 144 46
BsiHKAI G_wGCw'C
Cuts at: 401 503 1328 1358 3010 3671 3766 4136 4767
Size: 102 825 30 1652 661 95 370 631
Cuts at: 4767 5084 5095 5186 6587 7060 7214 7297 8075
Size: 317 11 91 1401 473 154 83 778
Cuts at: 8075 8265 9057 401
Size: 190 792 1627
Fragments arranged by size:
1652 1627 1401 825 792 778 661 631
473 370 317 190 154 102 95 91
83 30 11
BsiWI C'GTAC_G
Cuts at: 5507 5507
Size: 10283
BsmI GAATG_Cn'
Cuts at: 826 828 4069 7083 826
Size: 2 3241 3014 4026
Fragments arranged by size:
4026 3241 3014 2
BsmBI CGTCTCn'nnnn_
Cuts at: 1513 1513
Size: 10283
Bsp1286I G_dGCh'C
Cuts at: 401 503 520 1328 1358 2013 2136 2195 2328
Size: 102 17 808 30 655 123 59 133
Cuts at: 2328 2438 2481 2591 3010 3459 3486 3671 3766
Size: 110 43 110 419 449 27 185 95
Cuts at: 3766 3800 4136 4290 4733 4767 4878 4977 5084
Size: 34 336 154 443 34 111 99 107
Cuts at: 5084 5095 5186 5390 5538 5778 6551 6587 6641
Size: 11 91 204 148 240 773 36 54
Cuts at: 6641 6673 6951 7060 7136 7214 7297 7908 8001
Size: 32 278 109 76 78 83 611 93
Cuts at: 8001 8075 8265 8327 9057 401
Size: 74 190 62 730 1627
Fragments arranged by size:
1627 808 773 730 655 611 449 443
419 336 278 240 204 190 185 154
148 133 123 111 110 110 109 107
102 99 95 93 91 83 78 76
74 62 59 54 43 36 34 34
32 30 27 17 11
BspEI T'CCGG_A
Cuts at: 5580 9503 5580
Size: 3923 6360
BspHI T'CATG_A
Cuts at: 3758 4685 9459 3758
Size: 927 4774 4582
Fragments arranged by size:
4774 4582 927
BspMI ACCTGCnnnn'nnnn_
Cuts at: 386 670 779 1733 1820 4049 4196 4673 4774
Size: 284 109 954 87 2229 147 477 101
Cuts at: 4774 5471 5670 5897 6689 7333 7547 7849 8230
Size: 697 199 227 792 644 214 302 381
Cuts at: 8230 386
Size: 2439
Fragments arranged by size:
2439 2229 954 792 697 644 477 381
302 284 227 214 199 147 109 101
87
BsrBI CCG'CTC
Cuts at: 50 1308 1618 1683 1950 2180 2298 2601 3123
Size: 1258 310 65 267 230 118 303 522
Cuts at: 3123 3199 3453 4626 4755 5163 6348 6387 8575
Size: 76 254 1173 129 408 1185 39 2188
Cuts at: 8575 8629 10113 10278 50
Size: 54 1484 165 55
Fragments arranged by size:
2188 1484 1258 1185 1173 522 408 310
303 267 254 230 165 129 118 76
65 55 54 39
BsrDI GCAATG_nn'
Cuts at: 2846 5390 6692 7085 8195 2846
Size: 2544 1302 393 1110 4934
Fragments arranged by size:
4934 2544 1302 1110 393
BsrFI r'CCGG_y
Cuts at: 761 1097 1274 1763 2128 2183 2504 3112 3187
Size: 336 177 489 365 55 321 608 75
Cuts at: 3187 3241 3387 3473 3596 4550 5137 5699 5804
Size: 54 146 86 123 954 587 562 105
Cuts at: 5804 7125 8281 8462 8671 761
Size: 1321 1156 181 209 2373
Fragments arranged by size:
2373 1321 1156 954 608 587 562 489
365 336 321 209 181 177 146 123
105 86 75 55 54
BsrGI T'GTAC_A
Cuts at: 350 699 350
Size: 349 9934
BssSI C'ACGA_G
Cuts at: 1327 1447 1825 3970 4831 5077 5266 6271 7059
Size: 120 378 2145 861 246 189 1005 788
Cuts at: 7059 8554 8912 1327
Size: 1495 358 2698
Fragments arranged by size:
2698 2145 1495 1005 861 788 378 358
246 189 120
BstAPI GCAn_nnn'nTGC
Cuts at: 498 3957 4386 5001 5814 7087 7660 498
Size: 3459 429 615 813 1273 573 3121
Fragments arranged by size:
3459 3121 1273 813 615 573 429
BstBI TT'CG_AA
Cuts at: 2496 8644 2496
Size: 6148 4135
BstEII G'GTnAC_C
Cuts at: 2239 3498 2239
Size: 1259 9024
BstXI CCAn_nnnn'nTGG
Cuts at: 867 4452 4647 5376 867
Size: 3585 195 729 5774
Fragments arranged by size:
5774 3585 729 195
BstYI r'GATC_y
Cuts at: 1 443 590 870 3788 5234 5934 6056 7308
Size: 442 147 280 2918 1446 700 122 1252
Cuts at: 7308 7775 7798 8133 8379 9391 9477 9489 9835
Size: 467 23 335 246 1012 86 12 346
Cuts at: 9835 1
Size: 449
Fragments arranged by size:
2918 1446 1252 1012 700 467 449 442
346 335 280 246 147 122 86 23
12
Bsu36I CC'TnA_GG
Cuts at: 3256 3401 4747 3256
Size: 145 1346 8792
Fragments arranged by size:
8792 1346 145
BtgI C'CryG_G
Cuts at: 968 1007 1163 1565 2834 3621 3767 3792 4502
Size: 39 156 402 1269 787 146 25 710
Cuts at: 4502 4634 4646 5420 5501 5759 5930 6490 6918
Size: 132 12 774 81 258 171 560 428
Cuts at: 6918 968
Size: 4333
Fragments arranged by size:
4333 1269 787 774 710 560 428 402
258 171 156 146 132 81 39 25
12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1596 1830 3123 4607 4907 8233 8414 8577 1596
Size: 234 1293 1484 300 3326 181 163 3302
Fragments arranged by size:
3326 3302 1484 1293 300 234 181 163
BtsI GCAGTG_nn'
Cuts at: 447 472 1069 1562 2404 2731 3212 6299 7526
Size: 25 597 493 842 327 481 3087 1227
Cuts at: 7526 447
Size: 3204
Fragments arranged by size:
3204 3087 1227 842 597 493 481 327
25
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 117 152 2272 2307 117
Size: 35 2120 35 8093
Fragments arranged by size:
8093 2120 35 35
DraI TTT'AAA
Cuts at: 7282 7282
Size: 10283
DraIII CAC_nnn'GTG
Cuts at: 302 1632 5001 6520 302
Size: 1330 3369 1519 4065
Fragments arranged by size:
4065 3369 1519 1330
DrdI GACnn_nn'nnGTC
Cuts at: 2770 2893 7989 8847 2770
Size: 123 5096 858 4206
Fragments arranged by size:
5096 4206 858 123
EaeI y'GGCC_r
Cuts at: 763 965 1106 1385 1542 1580 1667 1955 2181
Size: 202 141 279 157 38 87 288 226
Cuts at: 2181 2231 2312 2506 3110 3189 3209 3389 3810
Size: 50 81 194 604 79 20 180 421
Cuts at: 3810 4076 4637 5018 5051 5378 5423 5498 5592
Size: 266 561 381 33 327 45 75 94
Cuts at: 5592 5694 5742 5762 6029 6047 6450 6493 6941
Size: 102 48 20 267 18 403 43 448
Cuts at: 6941 7868 8042 8433 8460 9805 763
Size: 927 174 391 27 1345 1241
Fragments arranged by size:
1345 1241 927 604 561 448 421 403
391 381 327 288 279 267 266 226
202 194 180 174 157 141 102 94
87 81 79 75 50 48 45 43
38 33 27 20 20 18
EagI C'GGCC_G
Cuts at: 1542 2181 5742 6047 7868 1542
Size: 639 3561 305 1821 3957
Fragments arranged by size:
3957 3561 1821 639 305
EarI CTCTTCn'nnn_
Cuts at: 1219 1745 3275 3535 6159 7115 8306 8516 1219
Size: 526 1530 260 2624 956 1191 210 2986
Fragments arranged by size:
2986 2624 1530 1191 956 526 260 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1336 1351 2128 4921 5098 5194 6405 8491 8801
Size: 15 777 2793 177 96 1211 2086 310
Cuts at: 8801 8947 10093 1336
Size: 146 1146 1526
Fragments arranged by size:
2793 2086 1526 1211 1146 777 310 177
146 96 15
EcoICRI GAG'CTC
Cuts at: 1356 4134 7058 7295 1356
Size: 2778 2924 237 4344
Fragments arranged by size:
4344 2924 2778 237
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 475 741 811 1333 1396 1492 1720 2038 2218
Size: 266 70 522 63 96 228 318 180
Cuts at: 2218 2230 2503 2617 2779 2788 2818 2884 3151
Size: 12 273 114 162 9 30 66 267
Cuts at: 3151 3398 3568 3703 3769 3823 3848 3997 4231
Size: 247 170 135 66 54 25 149 234
Cuts at: 4231 4312 4324 4879 5026 5164 5177 5689 5741
Size: 81 12 555 147 138 13 512 52
Cuts at: 5741 5767 6118 6343 6416 7130 7139 7239 8108
Size: 26 351 225 73 714 9 100 869
Cuts at: 8108 8540 9287 9555 475
Size: 432 747 268 1203
Fragments arranged by size:
1203 869 747 714 555 522 512 432
351 318 273 268 267 266 247 234
228 225 180 170 162 149 147 138
135 114 100 96 81 73 70 66
66 63 54 52 30 26 25 13
12 12 9 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2050 2212 2314 2485 3400 3490 3613 6617 2050
Size: 162 102 171 915 90 123 3004 5716
Fragments arranged by size:
5716 3004 915 171 162 123 102 90
EcoO109I rG'GnC_Cy
Cuts at: 186 345 960 1722 1779 1809 2191 2192 2307
Size: 159 615 762 57 30 382 1 115
Cuts at: 2307 2478 2693 2763 3063 3126 3252 3411 3552
Size: 171 215 70 300 63 126 159 141
Cuts at: 3552 4347 4520 4662 5616 5658 6438 6610 6638
Size: 795 173 142 954 42 780 172 28
Cuts at: 6638 6669 6830 6947 7201 7422 186
Size: 31 161 117 254 221 3047
Fragments arranged by size:
3047 954 795 780 762 615 382 300
254 221 215 173 172 171 161 159
159 142 141 126 117 115 70 63
57 42 31 30 28 1
EcoRI G'AATT_C
Cuts at: 7287 7287
Size: 10283
EcoRV GAT'ATC
Cuts at: 5102 5102
Size: 10283
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 309 5856 7020 8302 309
Size: 5547 1164 1282 2290
Fragments arranged by size:
5547 2290 1282 1164
FseI GG_CCGG'CC
Cuts at: 3391 3391
Size: 10283
FspI TGC'GCA
Cuts at: 1348 3322 6217 8064 1348
Size: 1974 2895 1847 3567
Fragments arranged by size:
3567 2895 1974 1847
HaeII r_GCGC'y
Cuts at: 1023 1446 1593 1883 2668 4200 4557 5175 5369
Size: 423 147 290 785 1532 357 618 194
Cuts at: 5369 5523 5628 6093 6224 7717 7965 8987 9966
Size: 154 105 465 131 1493 248 1022 979
Cuts at: 9966 1023
Size: 1340
Fragments arranged by size:
1532 1493 1340 1022 979 785 618 465
423 357 290 248 194 154 147 131
105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 205 237 575 607 619 651 769 801 892
Size: 32 338 32 12 32 118 32 91
Cuts at: 892 924 2431 2463 5911 5943 5980 6012 6055
Size: 32 1507 32 3448 32 37 32 43
Cuts at: 6055 6087 6648 6680 8125 8155 8157 8187 205
Size: 32 561 32 1445 30 2 30 2301
Fragments arranged by size:
3448 2301 1507 1445 561 338 118 91
43 37 32 32 32 32 32 32
32 32 32 32 30 30 12 2
HincII GTy'rAC
Cuts at: 2400 2898 4362 5886 6243 7322 2400
Size: 498 1464 1524 357 1079 5361
Fragments arranged by size:
5361 1524 1464 1079 498 357
HindIII A'AGCT_T
Cuts at: 1231 6328 1231
Size: 5097 5186
KasI G'GCGC_C
Cuts at: 4553 6089 7713 7961 4553
Size: 1536 1624 248 6875
Fragments arranged by size:
6875 1624 1536 248
KpnI G_GTAC'C
Cuts at: 7303 7303
Size: 10283
MluI A'CGCG_T
Cuts at: 9716 9716
Size: 10283
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1925 4151 8954 9138 1925
Size: 2226 4803 184 3070
Fragments arranged by size:
4803 3070 2226 184
MscI TGG'CCA
Cuts at: 1108 1957 3211 4639 5380 6031 6943 8044 1108
Size: 849 1254 1428 741 651 912 1101 3347
Fragments arranged by size:
3347 1428 1254 1101 912 849 741 651
MslI CAynn'nnrTG
Cuts at: 604 1331 1849 2836 3703 4331 4592 6010 7168
Size: 727 518 987 867 628 261 1418 1158
Cuts at: 7168 8272 8399 604
Size: 1104 127 2488
Fragments arranged by size:
2488 1418 1158 1104 987 867 727 628
518 261 127
MspA1I CmG'CkG
Cuts at: 182 719 1137 1383 1483 1541 1821 1938 1999
Size: 537 418 246 100 58 280 117 61
Cuts at: 1999 2275 2331 2836 3819 4323 5046 5503 5811
Size: 276 56 505 983 504 723 457 308
Cuts at: 5811 5932 6078 6103 6138 7394 7708 8068 9081
Size: 121 146 25 35 1256 314 360 1013
Cuts at: 9081 9326 182
Size: 245 1139
Fragments arranged by size:
1256 1139 1013 983 723 537 505 504
457 418 360 314 308 280 276 246
245 146 121 117 100 61 58 56
35 25
NaeI GCC'GGC
Cuts at: 1276 1765 2185 3114 3189 3389 3475 3598 4552
Size: 489 420 929 75 200 86 123 954
Cuts at: 4552 5139 5806 8464 1276
Size: 587 667 2658 3095
Fragments arranged by size:
3095 2658 954 929 667 587 489 420
200 123 86 75
NarI GG'CG_CC
Cuts at: 4554 6090 7714 7962 4554
Size: 1536 1624 248 6875
Fragments arranged by size:
6875 1624 1536 248
NcoI C'CATG_G
Cuts at: 1007 3767 4634 4646 1007
Size: 2760 867 12 6644
Fragments arranged by size:
6644 2760 867 12
NgoMIV G'CCGG_C
Cuts at: 1274 1763 2183 3112 3187 3387 3473 3596 4550
Size: 489 420 929 75 200 86 123 954
Cuts at: 4550 5137 5804 8462 1274
Size: 587 667 2658 3095
Fragments arranged by size:
3095 2658 954 929 667 587 489 420
200 123 86 75
NruI TCG'CGA
Cuts at: 4571 4571
Size: 10283
NsiI A_TGCA'T
Cuts at: 828 828
Size: 10283
NspI r_CATG'y
Cuts at: 441 3430 5293 5754 6991 7336 7836 8743 441
Size: 2989 1863 461 1237 345 500 907 1981
Fragments arranged by size:
2989 1981 1863 1237 907 500 461 345
PasI CC'CwG_GG
Cuts at: 1906 2245 3060 3486 3555 6951 1906
Size: 339 815 426 69 3396 5238
Fragments arranged by size:
5238 3396 815 426 339 69
PciI A'CATG_T
Cuts at: 6987 8739 6987
Size: 1752 8531
PflMI CCAn_nnn'nTGG
Cuts at: 453 719 1008 2226 2334 4836 5004 5046 5271
Size: 266 289 1218 108 2502 168 42 225
Cuts at: 5271 5547 453
Size: 276 5189
Fragments arranged by size:
5189 2502 1218 289 276 266 225 168
108 42
PfoI T'CCnGG_A
Cuts at: 362 1718 3800 5237 5855 362
Size: 1356 2082 1437 618 4790
Fragments arranged by size:
4790 2082 1437 1356 618
PmeI GTTT'AAAC
Cuts at: 7282 7282
Size: 10283
PmlI CAC'GTG
Cuts at: 332 332
Size: 10283
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4498 4530 5852 5884 9422 9454 4498
Size: 32 1322 32 3538 32 5327
Fragments arranged by size:
5327 3538 1322 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 186 345 1722 1779 1809 2763 3126 3252 4347
Size: 159 1377 57 30 954 363 126 1095
Cuts at: 4347 4662 6438 6610 6830 186
Size: 315 1776 172 220 3639
Fragments arranged by size:
3639 1776 1377 1095 954 363 315 220
172 159 126 57 30
PshAI GACnn'nnGTC
Cuts at: 3043 3043
Size: 10283
PspOMI G'GGCC_C
Cuts at: 2009 2191 2477 3455 5774 6637 6669 6947 2009
Size: 182 286 978 2319 863 32 278 5345
Fragments arranged by size:
5345 2319 978 863 286 278 182 32
PspXI vC'TCGA_Gb
Cuts at: 391 4494 6737 391
Size: 4103 2243 3937
Fragments arranged by size:
4103 3937 2243
PstI C_TGCA'G
Cuts at: 479 681 821 1064 1142 1575 1709 1772 1820
Size: 202 140 243 78 433 134 63 48
Cuts at: 1820 2621 3101 3311 4316 4562 4709 4808 5180
Size: 801 480 210 1005 246 147 99 372
Cuts at: 5180 5519 5624 5894 6083 6199 7330 8015 479
Size: 339 105 270 189 116 1131 685 2747
Fragments arranged by size:
2747 1131 1005 801 685 480 433 372
339 270 246 243 210 202 189 147
140 134 116 105 99 78 63 48
PvuI CG_AT'CG
Cuts at: 104 1082 104
Size: 978 9305
PvuII CAG'CTG
Cuts at: 719 1137 1383 1483 1821 1938 2275 3819 4323
Size: 418 246 100 338 117 337 1544 504
Cuts at: 4323 5046 5811 6078 6103 6138 7708 8068 719
Size: 723 765 267 25 35 1570 360 2934
Fragments arranged by size:
2934 1570 1544 765 723 504 418 360
338 337 267 246 117 100 35 25
RsrII CG'GwC_CG
Cuts at: 8478 8478
Size: 10283
SacI G_AGCT'C
Cuts at: 1358 4136 7060 7297 1358
Size: 2778 2924 237 4344
Fragments arranged by size:
4344 2924 2778 237
SacII CC_GC'GG
Cuts at: 2837 5504 5933 2837
Size: 2667 429 7187
Fragments arranged by size:
7187 2667 429
SalI G'TCGA_C
Cuts at: 6241 7320 6241
Size: 1079 9204
SapI GCTCTTCn'nnn_
Cuts at: 1219 7115 8306 8516 1219
Size: 5896 1191 210 2986
Fragments arranged by size:
5896 2986 1191 210
SbfI CC_TGCA'GG
Cuts at: 4316 4562 5180 7330 4316
Size: 246 618 2150 7269
Fragments arranged by size:
7269 2150 618 246
ScaI AGT'ACT
Cuts at: 9800 9800
Size: 10283
SexAI A'CCwGG_T
Cuts at: 4997 6440 4997
Size: 1443 8840
SfcI C'TryA_G
Cuts at: 33 475 677 817 1060 1138 1536 1571 1705
Size: 442 202 140 243 78 398 35 134
Cuts at: 1705 1768 1816 2617 3097 3307 4312 4558 4705
Size: 63 48 801 480 210 1005 246 147
Cuts at: 4705 4804 5176 5515 5620 5890 6079 6195 7326
Size: 99 372 339 105 270 189 116 1131
Cuts at: 7326 8011 9004 9195 33
Size: 685 993 191 1121
Fragments arranged by size:
1131 1121 1005 993 801 685 480 442
398 372 339 270 246 243 210 202
191 189 147 140 134 116 105 99
78 63 48 35
SfiI GGCCn_nnn'nGGCC
Cuts at: 3418 5422 3418
Size: 2004 8279
SfoI GGC'GCC
Cuts at: 4555 6091 7715 7963 4555
Size: 1536 1624 248 6875
Fragments arranged by size:
6875 1624 1536 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10283
SmaI CCC'GGG
Cuts at: 325 2626 3148 3505 5329 5917 5983 6643 7147
Size: 2301 522 357 1824 588 66 660 504
Cuts at: 7147 7159 7305 325
Size: 12 146 3303
Fragments arranged by size:
3303 2301 1824 660 588 522 504 357
146 66 12
SmlI C'TyrA_G
Cuts at: 391 484 2523 3164 4141 4372 4494 5467 6580
Size: 93 2039 641 977 231 122 973 1113
Cuts at: 6580 6737 7438 8353 8845 9107 391
Size: 157 701 915 492 262 1567
Fragments arranged by size:
2039 1567 1113 977 973 915 701 641
492 262 231 157 122 93
SpeI A'CTAG_T
Cuts at: 67 67
Size: 10283
SphI G_CATG'C
Cuts at: 441 7336 7836 441
Size: 6895 500 2888
Fragments arranged by size:
6895 2888 500
SrfI GCCC'GGGC
Cuts at: 2626 5983 2626
Size: 3357 6926
SspI AAT'ATT
Cuts at: 7024 7024
Size: 10283
StuI AGG'CCT
Cuts at: 2071 2260 2389 3022 3343 4912 5452 5713 5770
Size: 189 129 633 321 1569 540 261 57
Cuts at: 5770 6187 6277 6805 2071
Size: 417 90 528 5549
Fragments arranged by size:
5549 1569 633 540 528 417 321 261
189 129 90 57
StyI C'CwwG_G
Cuts at: 555 641 1007 2648 3066 3767 4229 4634 4646
Size: 86 366 1641 418 701 462 405 12
Cuts at: 4646 4958 6890 7037 7417 7771 555
Size: 312 1932 147 380 354 3067
Fragments arranged by size:
3067 1932 1641 701 462 418 405 380
366 354 312 147 86 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 141 2047 2064 2697 2917 3073 4237 4605 5099
Size: 1906 17 633 220 156 1164 368 494
Cuts at: 5099 5335 6531 7866 7999 8458 8667 141
Size: 236 1196 1335 133 459 209 1757
Fragments arranged by size:
1906 1757 1335 1196 1164 633 494 459
368 236 220 209 156 133 17
TatI w'GTAC_w
Cuts at: 161 350 699 6845 9798 161
Size: 189 349 6146 2953 646
Fragments arranged by size:
6146 2953 646 349 189
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 464 496 2693 2725 9800 9832 464
Size: 32 2197 32 7075 32 915
Fragments arranged by size:
7075 2197 915 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 8080 8080
Size: 10283
XbaI T'CTAG_A
Cuts at: 7314 7314
Size: 10283
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1462 2427 3744 5043 7778 1462
Size: 965 1317 1299 2735 3967
Fragments arranged by size:
3967 2735 1317 1299 965
XhoI C'TCGA_G
Cuts at: 391 3164 4494 6737 391
Size: 2773 1330 2243 3937
Fragments arranged by size:
3937 2773 2243 1330
XmaI C'CCGG_G
Cuts at: 323 2624 3146 3503 5327 5915 5981 6641 7145
Size: 2301 522 357 1824 588 66 660 504
Cuts at: 7145 7157 7303 323
Size: 12 146 3303
Fragments arranged by size:
3303 2301 1824 660 588 522 504 357
146 66 12
XmnI GAAnn'nnTTC
Cuts at: 292 826 1714 3686 292
Size: 534 888 1972 6889
Fragments arranged by size:
6889 1972 888 534
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AscI BaeI BmtI BssHII BstZ17I
ClaI FspAI HpaI MfeI NdeI NheI NotI PacI
PsiI PsrI SanDI SgrAI SnaBI SwaI ZraI