Restriction map for
pF1KA0322
(Circular) (Six-base) MAPSORT of: pF1KA0322.seq Check: 1718 from: 1 to: 7931
pF1KA0322 7931 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3058 3058
Size: 7931
AccI GT'mk_AC
Cuts at: 4508 4969 4508
Size: 461 7470
Acc65I G'GTAC_C
Cuts at: 2352 4947 2352
Size: 2595 5336
AclI AA'CG_TT
Cuts at: 7771 7771
Size: 7931
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 814 1433 3250 5756 6188 6541 7203 814
Size: 619 1817 2506 432 353 662 1542
Fragments arranged by size:
2506 1817 1542 662 619 432 353
AfeI AGC'GCT
Cuts at: 994 3333 4669 994
Size: 2339 1336 4256
Fragments arranged by size:
4256 2339 1336
AflIII A'CryG_T
Cuts at: 2129 3140 5283 7083 7364 2129
Size: 1011 2143 1800 281 2696
Fragments arranged by size:
2696 2143 1800 1011 281
AhdI GACnn_n'nnGTC
Cuts at: 2388 2388
Size: 7931
AleI CACnn'nnGTG
Cuts at: 937 4396 937
Size: 3459 4472
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4842 4842
Size: 7931
AlwNI CAG_nnn'CTG
Cuts at: 393 1709 4689 5236 6674 393
Size: 1316 2980 547 1438 1650
Fragments arranged by size:
2980 1650 1438 1316 547
ApaI G_GGCC'C
Cuts at: 615 615
Size: 7931
ApaLI G'TGCA_C
Cuts at: 6769 6769
Size: 7931
ApoI r'AATT_y
Cuts at: 1196 3129 4264 4935 7284 7540 1196
Size: 1933 1135 671 2349 256 1587
Fragments arranged by size:
2349 1933 1587 1135 671 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7931
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7931
AvaI C'yCGr_G
Cuts at: 1765 1966 2989 3170 4512 4951 1765
Size: 201 1023 181 1342 439 4745
Fragments arranged by size:
4745 1342 1023 439 201 181
BamHI G'GATC_C
Cuts at: 4956 4956
Size: 7931
BanI G'GyrC_C
Cuts at: 1257 2352 4564 4947 5361 5609 5644 1257
Size: 1095 2212 383 414 248 35 3544
Fragments arranged by size:
3544 2212 1095 414 383 248 35
BanII G_rGCy'C
Cuts at: 615 1430 1565 1907 1967 2374 3765 3932 4028
Size: 815 135 342 60 407 1391 167 96
Cuts at: 4028 4945 5975 615
Size: 917 1030 2571
Fragments arranged by size:
2571 1391 1030 917 815 407 342 167
135 96 60
BbeI G_GCGC'C
Cuts at: 5365 5613 5365
Size: 248 7683
BbsI GAAGACnn'nnnn_
Cuts at: 1535 2295 3027 3271 1535
Size: 760 732 244 6195
Fragments arranged by size:
6195 760 732 244
BbvCI CC'TCA_GC
Cuts at: 1355 1355
Size: 7931
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 191 225 2226 2260 90
Size: 34 67 34 2001 34 5761
Fragments arranged by size:
5761 2001 67 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4865 5829 6885 7931 4865
Size: 964 1056 1046 4865
Fragments arranged by size:
4865 1056 1046 964
BclI T'GATC_A
Cuts at: 719 5451 719
Size: 4732 3199
BglI GCCn_nnn'nGGC
Cuts at: 172 172
Size: 7931
BglII A'GATC_T
Cuts at: 578 2285 2898 5446 7483 578
Size: 1707 613 2548 2037 1026
Fragments arranged by size:
2548 2037 1707 1026 613
BlpI GC'TnA_GC
Cuts at: 1906 3522 5043 1906
Size: 1616 1521 4794
Fragments arranged by size:
4794 1616 1521
Bme1580I G_kGCm'C
Cuts at: 383 615 2444 4730 4821 5556 5649 6773 383
Size: 232 1829 2286 91 735 93 1124 1541
Fragments arranged by size:
2286 1829 1541 1124 735 232 93 91
BmgBI CAC'GTC
Cuts at: 939 2177 939
Size: 1238 6693
BmrI ACTGGGnnnn_n'
Cuts at: 414 626 1460 2619 2880 5143 5224 5559 7703
Size: 212 834 1159 261 2263 81 335 2144
Cuts at: 7703 414
Size: 642
Fragments arranged by size:
2263 2144 1159 834 642 335 261 212
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2100 6298 2100
Size: 4198 3733
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1054 1672 1753 1964 2070 2909 3295 3356 3916
Size: 618 81 211 106 839 386 61 560
Cuts at: 3916 4400 4567 1054
Size: 484 167 4418
Fragments arranged by size:
4418 839 618 560 484 386 211 167
106 81 61
Bpu10I CC'TnA_GC
Cuts at: 1355 2375 1355
Size: 1020 6911
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1864 3607 4141 4942 5107 5986 6700 6998 1864
Size: 1743 534 801 165 879 714 298 2797
Fragments arranged by size:
2797 1743 879 801 714 534 298 165
BsaI GGTCTCn'nnnn_
Cuts at: 682 1379 1404 1762 7635 682
Size: 697 25 358 5873 978
Fragments arranged by size:
5873 978 697 358 25
BsaAI yAC'GTr
Cuts at: 2445 2658 5284 2445
Size: 213 2626 5092
Fragments arranged by size:
5092 2626 213
BsaBI GATnn'nnATC
Cuts at: 6 3608 4631 5445 7482 6
Size: 3602 1023 814 2037 455
Fragments arranged by size:
3602 2037 1023 814 455
BsaHI Gr'CG_yC
Cuts at: 175 204 287 5362 5610 6312 7808 175
Size: 29 83 5075 248 702 1496 298
Fragments arranged by size:
5075 1496 702 298 248 83 29
BsaWI w'CCGG_w
Cuts at: 5120 5641 6319 6730 6877 7151 5120
Size: 521 678 411 147 274 5900
Fragments arranged by size:
5900 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 325 355 1855 1885 2206 2236 2490 2520 3293
Size: 30 1500 30 321 30 254 30 773
Cuts at: 3293 3323 4360 4390 325
Size: 30 1037 30 3866
Fragments arranged by size:
3866 1500 1037 773 321 254 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 873 1174 1519 1573 1585 1627 1642 1645 1861
Size: 301 345 54 12 42 15 3 216
Cuts at: 1861 1864 1878 1879 1882 2151 2256 2298 2338
Size: 3 14 1 3 269 105 42 40
Cuts at: 2338 2341 2344 2858 3380 3982 4397 873
Size: 3 3 514 522 602 415 4407
Fragments arranged by size:
4407 602 522 514 415 345 301 269
216 105 54 42 42 40 15 14
12 3 3 3 3 3 1
BseYI C'CCAG_C
Cuts at: 430 1293 1420 1542 2020 2062 2404 2531 2835
Size: 863 127 122 478 42 342 127 304
Cuts at: 2835 2964 3176 5356 6114 6779 7187 430
Size: 129 212 2180 758 665 408 1174
Fragments arranged by size:
2180 1174 863 758 665 478 408 342
304 212 129 127 127 122 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 100 1490 1792 4102 100
Size: 1390 302 2310 3929
Fragments arranged by size:
3929 2310 1390 302
BsiEI CG_ry'CG
Cuts at: 104 1317 3276 4143 5473 5519 6749 104
Size: 1213 1959 867 1330 46 1230 1286
Fragments arranged by size:
1959 1330 1286 1230 1213 867 46
BsiHKAI G_wGCw'C
Cuts at: 1430 2299 3932 4028 4945 5723 5913 6773 1430
Size: 869 1633 96 917 778 190 860 2588
Fragments arranged by size:
2588 1633 917 869 860 778 190 96
BsmBI CGTCTCn'nnnn_
Cuts at: 182 1729 182
Size: 1547 6384
Bsp1286I G_dGCh'C
Cuts at: 383 615 1430 1565 1907 1967 2299 2374 2444
Size: 232 815 135 342 60 332 75 70
Cuts at: 2444 3765 3932 4028 4730 4821 4945 5556 5649
Size: 1321 167 96 702 91 124 611 93
Cuts at: 5649 5723 5913 5975 6773 383
Size: 74 190 62 798 1541
Fragments arranged by size:
1541 1321 815 798 702 611 342 332
232 190 167 135 124 96 93 91
75 74 70 62 60
BspHI T'CATG_A
Cuts at: 6363 6363
Size: 7931
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2473 3058 4981 5195 5497 5878 2473
Size: 585 1923 214 302 381 4526
Fragments arranged by size:
4526 1923 585 381 302 214
BsrBI CCG'CTC
Cuts at: 50 222 302 341 6223 6277 7761 7926 50
Size: 172 80 39 5882 54 1484 165 55
Fragments arranged by size:
5882 1484 172 165 80 55 54 39
BsrDI GCAATG_nn'
Cuts at: 2780 3436 4767 5843 2780
Size: 656 1331 1076 4868
Fragments arranged by size:
4868 1331 1076 656
BsrFI r'CCGG_y
Cuts at: 206 5929 6110 206
Size: 5723 181 2027
Fragments arranged by size:
5723 2027 181
BssSI C'ACGA_G
Cuts at: 2148 2163 2193 2208 4194 6202 6910 2148
Size: 15 30 15 1986 2008 708 3169
Fragments arranged by size:
3169 2008 1986 708 30 15 15
BstAPI GCAn_nnn'nTGC
Cuts at: 1709 1815 2180 2195 5308 1709
Size: 106 365 15 3113 4332
Fragments arranged by size:
4332 3113 365 106 15
BstBI TT'CG_AA
Cuts at: 3414 6292 3414
Size: 2878 5053
BstEII G'GTnAC_C
Cuts at: 324 4618 324
Size: 4294 3637
BstXI CCAn_nnnn'nTGG
Cuts at: 351 2609 4545 351
Size: 2258 1936 3737
Fragments arranged by size:
3737 2258 1936
BstYI r'GATC_y
Cuts at: 1 578 862 2285 2898 3609 4842 4956 5423
Size: 577 284 1423 613 711 1233 114 467
Cuts at: 5423 5446 5781 6027 6333 6345 6431 7483 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1423 1233 1052 711 613 577 467 449
335 306 284 246 114 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 265 2557 265
Size: 2292 5639
BtgI C'CryG_G
Cuts at: 170 498 597 749 846 1774 2270 2410 2477
Size: 328 99 152 97 928 496 140 67
Cuts at: 2477 2631 4568 4752 170
Size: 154 1937 184 3349
Fragments arranged by size:
3349 1937 928 496 328 184 154 152
140 99 97 67
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5881 6062 6225 5881
Size: 181 163 7587
Fragments arranged by size:
7587 181 163
BtsI GCAGTG_nn'
Cuts at: 1242 1311 1516 4288 5174 1242
Size: 69 205 2772 886 3999
Fragments arranged by size:
3999 2772 886 205 69
ClaI AT'CG_AT
Cuts at: 542 2588 542
Size: 2046 5885
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3228 3263 3228
Size: 35 7896
DraI TTT'AAA
Cuts at: 669 4930 669
Size: 4261 3670
DrdI GACnn_nn'nnGTC
Cuts at: 707 5637 6981 707
Size: 4930 1344 1657
Fragments arranged by size:
4930 1657 1344
EaeI y'GGCC_r
Cuts at: 164 1077 1481 1925 1998 2027 2426 3380 5516
Size: 913 404 444 73 29 399 954 2136
Cuts at: 5516 5690 6081 6108 7453 164
Size: 174 391 27 1345 642
Fragments arranged by size:
2136 1345 954 913 642 444 404 399
391 174 73 29 27
EagI C'GGCC_G
Cuts at: 5516 5516
Size: 7931
EarI CTCTTCn'nnn_
Cuts at: 1469 1604 1687 1943 1955 2603 3269 3635 3774
Size: 135 83 256 12 648 666 366 139
Cuts at: 3774 4033 4334 5954 6164 1469
Size: 259 301 1620 210 3236
Fragments arranged by size:
3236 1620 666 648 366 301 259 256
210 139 135 83 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1812 2864 4608 6139 6881 7027 7741 1812
Size: 1052 1744 1531 742 146 714 2002
Fragments arranged by size:
2002 1744 1531 1052 742 714 146
EcoICRI GAG'CTC
Cuts at: 1428 3930 4026 4943 1428
Size: 2502 96 917 4416
Fragments arranged by size:
4416 2502 917 96
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 814 1054 1433 1672 1753 1964 2070 2909 3250
Size: 240 379 239 81 211 106 839 341
Cuts at: 3250 3295 3356 3916 4400 4567 5756 6188 6541
Size: 45 61 560 484 167 1189 432 353
Cuts at: 6541 7203 814
Size: 662 1542
Fragments arranged by size:
1542 1189 839 662 560 484 432 379
353 341 240 239 211 167 106 81
61 45
EcoNI CCTnn'n_nnAGG
Cuts at: 1435 1501 1654 1948 1435
Size: 66 153 294 7418
Fragments arranged by size:
7418 294 153 66
EcoO109I rG'GnC_Cy
Cuts at: 390 611 612 1408 1706 1761 3972 4199 5070
Size: 221 1 796 298 55 2211 227 871
Cuts at: 5070 390
Size: 3251
Fragments arranged by size:
3251 2211 871 796 298 227 221 55
1
EcoRI G'AATT_C
Cuts at: 4264 4935 4264
Size: 671 7260
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 600 1014 3782 5950 600
Size: 414 2768 2168 2581
Fragments arranged by size:
2768 2581 2168 414
FspI TGC'GCA
Cuts at: 5712 5712
Size: 7931
HaeII r_GCGC'y
Cuts at: 996 2207 2282 2405 3335 4483 4671 4776 5365
Size: 1211 75 123 930 1148 188 105 589
Cuts at: 5365 5613 6843 7614 996
Size: 248 1230 771 1313
Fragments arranged by size:
1313 1230 1211 1148 930 771 589 248
188 123 105 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 325 357 454 486 535 567 847 854 879
Size: 32 97 32 49 32 280 7 25
Cuts at: 879 886 1115 1147 1171 1203 1495 1527 1855
Size: 7 229 32 24 32 292 32 328
Cuts at: 1855 1887 2374 2406 3293 3325 3580 3612 3634
Size: 32 487 32 887 32 255 32 22
Cuts at: 3634 3666 4360 4392 5773 5803 5805 5835 325
Size: 32 694 32 1381 30 2 30 2421
Fragments arranged by size:
2421 1381 887 694 487 328 292 280
255 229 97 49 32 32 32 32
32 32 32 32 32 32 32 32
30 30 25 24 22 7 7 2
HincII GTy'rAC
Cuts at: 4889 4970 4889
Size: 81 7850
HindIII A'AGCT_T
Cuts at: 3519 3519
Size: 7931
HpaI GTT'AAC
Cuts at: 4889 4889
Size: 7931
KasI G'GCGC_C
Cuts at: 5361 5609 5361
Size: 248 7683
KpnI G_GTAC'C
Cuts at: 2356 4951 2356
Size: 2595 5336
MluI A'CGCG_T
Cuts at: 2129 7364 2129
Size: 5235 2696
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 691 1114 2710 3102 3866 3942 6690 6874 691
Size: 423 1596 392 764 76 2748 184 1748
Fragments arranged by size:
2748 1748 1596 764 423 392 184 76
MscI TGG'CCA
Cuts at: 2000 2029 3382 5692 2000
Size: 29 1353 2310 4239
Fragments arranged by size:
4239 2310 1353 29
MslI CAynn'nnrTG
Cuts at: 349 937 1103 1151 3139 3428 4396 4824 5920
Size: 588 166 48 1988 289 968 428 1096
Cuts at: 5920 6047 349
Size: 127 2233
Fragments arranged by size:
2233 1988 1096 968 588 428 289 166
127 48
MspA1I CmG'CkG
Cuts at: 1113 1293 1354 1491 1677 1776 1930 2968 3704
Size: 180 61 137 186 99 154 1038 736
Cuts at: 3704 4570 4646 5042 5356 5716 6500 6745 1113
Size: 866 76 396 314 360 784 245 2299
Fragments arranged by size:
2299 1038 866 784 736 396 360 314
245 186 180 154 137 99 76 61
NaeI GCC'GGC
Cuts at: 6112 6112
Size: 7931
NarI GG'CG_CC
Cuts at: 5362 5610 5362
Size: 248 7683
NcoI C'CATG_G
Cuts at: 170 597 749 846 170
Size: 427 152 97 7255
Fragments arranged by size:
7255 427 152 97
NgoMIV G'CCGG_C
Cuts at: 6110 6110
Size: 7931
NspI r_CATG'y
Cuts at: 450 1818 2715 3013 3144 4829 4984 5484 7087
Size: 1368 897 298 131 1685 155 500 1603
Cuts at: 7087 450
Size: 1294
Fragments arranged by size:
1685 1603 1368 1294 897 500 298 155
131
PasI CC'CwG_GG
Cuts at: 1827 3682 4814 1827
Size: 1855 1132 4944
Fragments arranged by size:
4944 1855 1132
PciI A'CATG_T
Cuts at: 3140 7083 3140
Size: 3943 3988
PflMI CCAn_nnn'nTGG
Cuts at: 258 750 1395 2739 258
Size: 492 645 1344 5450
Fragments arranged by size:
5450 1344 645 492
PfoI T'CCnGG_A
Cuts at: 1031 1031
Size: 7931
PmeI GTTT'AAAC
Cuts at: 4930 4930
Size: 7931
PmlI CAC'GTG
Cuts at: 2445 2658 2445
Size: 213 7718
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6377 6409 6377
Size: 32 7899
PpuMI rG'GwC_Cy
Cuts at: 390 1408 1761 390
Size: 1018 353 6560
Fragments arranged by size:
6560 1018 353
PspOMI G'GGCC_C
Cuts at: 611 611
Size: 7931
PspXI vC'TCGA_Gb
Cuts at: 4512 4512
Size: 7931
PstI C_TGCA'G
Cuts at: 1676 2143 2960 3668 4703 4978 5663 1676
Size: 467 817 708 1035 275 685 3944
Fragments arranged by size:
3944 1035 817 708 685 467 275
PvuI CG_AT'CG
Cuts at: 104 1317 104
Size: 1213 6718
PvuII CAG'CTG
Cuts at: 1113 1293 1354 1491 1677 5356 5716 1113
Size: 180 61 137 186 3679 360 3328
Fragments arranged by size:
3679 3328 360 186 180 137 61
RsrII CG'GwC_CG
Cuts at: 6126 6126
Size: 7931
SacI G_AGCT'C
Cuts at: 1430 3932 4028 4945 1430
Size: 2502 96 917 4416
Fragments arranged by size:
4416 2502 917 96
SacII CC_GC'GG
Cuts at: 1777 4571 1777
Size: 2794 5137
SalI G'TCGA_C
Cuts at: 4968 4968
Size: 7931
SanDI GG'GwC_CC
Cuts at: 1761 1761
Size: 7931
SapI GCTCTTCn'nnn_
Cuts at: 1469 1955 4033 5954 6164 1469
Size: 486 2078 1921 210 3236
Fragments arranged by size:
3236 2078 1921 486 210
SbfI CC_TGCA'GG
Cuts at: 3668 4978 3668
Size: 1310 6621
ScaI AGT'ACT
Cuts at: 4057 7448 4057
Size: 3391 4540
SexAI A'CCwGG_T
Cuts at: 320 392 320
Size: 72 7859
SfcI C'TryA_G
Cuts at: 33 1672 2139 2154 2169 2184 2199 2214 2956
Size: 1639 467 15 15 15 15 15 742
Cuts at: 2956 3664 3810 3981 4699 4974 5659 6627 6818
Size: 708 146 171 718 275 685 968 191
Cuts at: 6818 33
Size: 1146
Fragments arranged by size:
1639 1146 968 742 718 708 685 467
275 191 171 146 15 15 15 15
15
SfoI GGC'GCC
Cuts at: 5363 5611 5363
Size: 248 7683
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7931
SgrAI Cr'CCGG_yG
Cuts at: 206 206
Size: 7931
SmaI CCC'GGG
Cuts at: 3172 4953 3172
Size: 1781 6150
SmlI C'TyrA_G
Cuts at: 1879 3586 4120 4512 4921 5086 6001 6715 6977
Size: 1707 534 392 409 165 915 714 262
Cuts at: 6977 1879
Size: 2833
Fragments arranged by size:
2833 1707 915 714 534 409 392 262
165
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7931
SphI G_CATG'C
Cuts at: 1818 2715 4984 5484 1818
Size: 897 2269 500 4265
Fragments arranged by size:
4265 2269 897 500
SspI AAT'ATT
Cuts at: 4252 4252
Size: 7931
StuI AGG'CCT
Cuts at: 161 2562 3343 161
Size: 2401 781 4749
Fragments arranged by size:
4749 2401 781
StyI C'CwwG_G
Cuts at: 170 597 696 749 846 1553 1565 5065 5419
Size: 427 99 53 97 707 12 3500 354
Cuts at: 5419 170
Size: 2682
Fragments arranged by size:
3500 2682 707 427 354 99 97 53
12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1163 1238 1285 1489 4489 5514 5647 6106 6315
Size: 75 47 204 3000 1025 133 459 209
Cuts at: 6315 1163
Size: 2779
Fragments arranged by size:
3000 2779 1025 459 209 204 133 75
47
TatI w'GTAC_w
Cuts at: 4055 4421 7446 4055
Size: 366 3025 4540
Fragments arranged by size:
4540 3025 366
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1772 1804 2490 2522 7448 7480 1772
Size: 32 686 32 4926 32 2223
Fragments arranged by size:
4926 2223 686 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 322 394 5728 322
Size: 72 5334 2525
Fragments arranged by size:
5334 2525 72
XbaI T'CTAG_A
Cuts at: 184 3355 4962 184
Size: 3171 1607 3153
Fragments arranged by size:
3171 3153 1607
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 604 939 1594 2736 5426 604
Size: 335 655 1142 2690 3109
Fragments arranged by size:
3109 2690 1142 655 335
XhoI C'TCGA_G
Cuts at: 4512 4512
Size: 7931
XmaI C'CCGG_G
Cuts at: 3170 4951 3170
Size: 1781 6150
XmnI GAAnn'nnTTC
Cuts at: 479 479
Size: 7931
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AloI AscI AvrII BaeI BfrBI
BmtI BsiWI BsmI BspEI BsrGI BssHII BstZ17I DraIII
EcoRV FseI FspAI MfeI NdeI NheI NotI NruI
NsiI PacI PshAI PsiI PsrI SfiI SnaBI SrfI
SwaI ZraI