Restriction map for
pF1KA0325
(Circular) (Six-base) MAPSORT of: pF1KA0325.seq Check: 5914 from: 1 to: 17051
pF1KA0325 17051 bp
With 182 enzymes: SgfI *
May 20, 2014 13:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 215 3801 4056 4793 6018 12051 12371 215
Size: 3586 255 737 1225 6033 320 4895
Fragments arranged by size:
6033 4895 3586 1225 737 320 255
AatII G_ACGT'C
Cuts at: 371 371
Size: 17051
AccI GT'mk_AC
Cuts at: 3581 4742 8759 9743 12971 13934 14089 3581
Size: 1161 4017 984 3228 963 155 6543
Fragments arranged by size:
6543 4017 3228 1161 984 963 155
Acc65I G'GTAC_C
Cuts at: 3116 14067 3116
Size: 10951 6100
AclI AA'CG_TT
Cuts at: 16891 16891
Size: 17051
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 632 1381 1465 2173 2918 3256 3263 3922 3997
Size: 749 84 708 745 338 7 659 75
Cuts at: 3997 6314 6564 6673 6841 7908 8023 8692 8818
Size: 2317 250 109 168 1067 115 669 126
Cuts at: 8818 10564 10947 11446 11665 11803 12415 13159 13363
Size: 1746 383 499 219 138 612 744 204
Cuts at: 13363 13536 14876 15308 16055 16323 632
Size: 173 1340 432 747 268 1360
Fragments arranged by size:
2317 1746 1360 1340 1067 749 747 745
744 708 669 659 612 499 432 383
338 268 250 219 204 173 168 138
126 115 109 84 75 7
AfeI AGC'GCT
Cuts at: 349 1033 2611 3691 7570 8534 349
Size: 684 1578 1080 3879 964 8866
Fragments arranged by size:
8866 3879 1578 1080 964 684
AflII C'TTAA_G
Cuts at: 2305 2305
Size: 17051
AflIII A'CryG_T
Cuts at: 185 6596 6951 10220 10319 12125 12267 12767 13631
Size: 6411 355 3269 99 1806 142 500 864
Cuts at: 13631 14403 15507 16484 185
Size: 772 1104 977 752
Fragments arranged by size:
6411 3269 1806 1104 977 864 772 752
500 355 142 99
AgeI A'CCGG_T
Cuts at: 15439 15439
Size: 17051
AhdI GACnn_n'nnGTC
Cuts at: 1956 12744 13212 1956
Size: 10788 468 5795
Fragments arranged by size:
10788 5795 468
AleI CACnn'nnGTG
Cuts at: 1898 2021 3622 6623 6952 7675 12208 12705 1898
Size: 123 1601 3001 329 723 4533 497 6244
Fragments arranged by size:
6244 4533 3001 1601 723 497 329 123
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 235 10369 12479 14052 235
Size: 10134 2110 1573 3234
Fragments arranged by size:
10134 3234 2110 1573
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2494 2526 12907 12939 13318 13350 2494
Size: 32 10381 32 379 32 6195
Fragments arranged by size:
10381 6195 379 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 916 2151 2550 2623 4033 5491 5851 7801 8260
Size: 1235 399 73 1410 1458 360 1950 459
Cuts at: 8260 8732 10017 10362 11673 11886 12897 13186 13290
Size: 472 1285 345 1311 213 1011 289 104
Cuts at: 13290 14356 15923 916
Size: 1066 1567 2044
Fragments arranged by size:
2044 1950 1567 1458 1410 1311 1285 1235
1066 1011 472 459 399 360 345 289
213 104 73
ApaI G_GGCC'C
Cuts at: 6782 8329 6782
Size: 1547 15504
ApaLI G'TGCA_C
Cuts at: 10982 14037 15821 10982
Size: 3055 1784 12212
Fragments arranged by size:
12212 3055 1784
ApoI r'AATT_y
Cuts at: 397 710 872 1330 1510 3157 3496 3529 4191
Size: 313 162 458 180 1647 339 33 662
Cuts at: 4191 4489 5701 9430 10900 11317 11818 12724 14055
Size: 298 1212 3729 1470 417 501 906 1331
Cuts at: 14055 16404 16660 397
Size: 2349 256 788
Fragments arranged by size:
3729 2349 1647 1470 1331 1212 906 788
662 501 458 417 339 313 298 256
180 162 33
AseI AT'TA_AT
Cuts at: 20 20
Size: 17051
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 17051
AvaI C'yCGr_G
Cuts at: 118 2247 2276 5141 6783 6838 8322 11098 11150
Size: 2129 29 2865 1642 55 1484 2776 52
Cuts at: 11150 11189 11723 14071 118
Size: 39 534 2348 3098
Fragments arranged by size:
3098 2865 2776 2348 2129 1642 1484 534
55 52 39 29
BamHI G'GATC_C
Cuts at: 863 3101 7029 11154 11876 13326 13998 14076 863
Size: 2238 3928 4125 722 1450 672 78 3838
Fragments arranged by size:
4125 3928 3838 2238 1450 722 672 78
BanI G'GyrC_C
Cuts at: 219 249 3116 5962 6543 7761 8353 9156 11788
Size: 30 2867 2846 581 1218 592 803 2632
Cuts at: 11788 12507 13223 13512 13606 13756 14067 14481 14729
Size: 719 716 289 94 150 311 414 248
Cuts at: 14729 14764 219
Size: 35 2506
Fragments arranged by size:
2867 2846 2632 2506 1218 803 719 716
592 581 414 311 289 248 150 94
35 30
BanII G_rGCy'C
Cuts at: 119 991 3674 6136 6782 6944 7477 8329 9257
Size: 872 2683 2462 646 162 533 852 928
Cuts at: 9257 9722 10340 11804 13724 14065 15095 119
Size: 465 618 1464 1920 341 1030 2075
Fragments arranged by size:
2683 2462 2075 1920 1464 1030 928 872
852 646 618 533 465 341 162
BbeI G_GCGC'C
Cuts at: 253 13227 13610 13760 14485 14733 253
Size: 12974 383 150 725 248 2571
Fragments arranged by size:
12974 2571 725 383 248 150
BbsI GAAGACnn'nnnn_
Cuts at: 494 539 824 2619 3901 6367 7005 7102 8011
Size: 45 285 1795 1282 2466 638 97 909
Cuts at: 8011 9023 10777 13201 494
Size: 1012 1754 2424 4344
Fragments arranged by size:
4344 2466 2424 1795 1754 1282 1012 909
638 285 97 45
BbvCI CC'TCA_GC
Cuts at: 1631 8374 8866 12580 1631
Size: 6743 492 3714 6102
Fragments arranged by size:
6743 6102 3714 492
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 5028 5062 8647 8681 12262 12296 5028
Size: 34 3585 34 3581 34 9783
Fragments arranged by size:
9783 3585 3581 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8460 10874 14949 15710 17051 8460
Size: 2414 4075 761 1341 8460
Fragments arranged by size:
8460 4075 2414 1341 761
BclI T'GATC_A
Cuts at: 743 2660 5582 8013 10871 11090 13124 13460 14571
Size: 1917 2922 2431 2858 219 2034 336 1111
Cuts at: 14571 743
Size: 3223
Fragments arranged by size:
3223 2922 2858 2431 2034 1917 1111 336
219
BfrBI ATG'CAT
Cuts at: 1314 1314
Size: 17051
BglI GCCn_nnn'nGGC
Cuts at: 1747 4012 5797 6243 7936 10492 13065 13643 1747
Size: 2265 1785 446 1693 2556 2573 578 5155
Fragments arranged by size:
5155 2573 2556 2265 1785 1693 578 446
BglII A'GATC_T
Cuts at: 2198 3330 3542 8345 9030 9948 11178 14566 16603
Size: 1132 212 4803 685 918 1230 3388 2037
Cuts at: 16603 2198
Size: 2646
Fragments arranged by size:
4803 3388 2646 2037 1230 1132 918 685
212
BlpI GC'TnA_GC
Cuts at: 2613 5452 5623 7213 11920 13291 14163 2613
Size: 2839 171 1590 4707 1371 872 5501
Fragments arranged by size:
5501 4707 2839 1590 1371 872 171
Bme1580I G_kGCm'C
Cuts at: 1239 1300 1735 6782 8329 8586 9219 10986 11791
Size: 61 435 5047 1547 257 633 1767 805
Cuts at: 11791 12020 12205 12639 14041 14676 14769 15825 1239
Size: 229 185 434 1402 635 93 1056 2465
Fragments arranged by size:
5047 2465 1767 1547 1402 1056 805 635
633 435 434 257 229 185 93 61
BmgBI CAC'GTC
Cuts at: 186 6597 7391 9297 13214 186
Size: 6411 794 1906 3917 4023
Fragments arranged by size:
6411 4023 3917 1906 794
BmrI ACTGGGnnnn_n'
Cuts at: 2220 3897 4043 5846 8749 12712 12846 14263 14344
Size: 1677 146 1803 2903 3963 134 1417 81
Cuts at: 14344 14679 16823 2220
Size: 335 2144 2448
Fragments arranged by size:
3963 2903 2448 2144 1803 1677 1417 335
146 134 81
BmtI G_CTAG'C
Cuts at: 9592 9592
Size: 17051
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 159 3349 3591 5133 6363 7120 10553 15418 159
Size: 3190 242 1542 1230 757 3433 4865 1792
Fragments arranged by size:
4865 3433 3190 1792 1542 1230 757 242
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 283 295 313 869 1848 5105 6016 6626
Size: 30 12 18 556 979 3257 911 610
Cuts at: 6626 6715 7456 7585 7637 7959 7973 8236 8566
Size: 89 741 129 52 322 14 263 330
Cuts at: 8566 9142 9472 9709 9814 10090 11437 11578 12048
Size: 576 330 237 105 276 1347 141 470
Cuts at: 12048 12454 12934 13735 253
Size: 406 480 801 3569
Fragments arranged by size:
3569 3257 1347 979 911 801 741 610
576 556 480 470 406 330 330 322
276 263 237 141 129 105 89 52
30 18 14 12
Bpu10I CC'TnA_GC
Cuts at: 1631 5651 8374 8866 9721 12580 1631
Size: 4020 2723 492 855 2859 6102
Fragments arranged by size:
6102 4020 2859 2723 855 492
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1290 8879 9316 9792 11545 11853 13285 14227 15106
Size: 7589 437 476 1753 308 1432 942 879
Cuts at: 15106 15598 15896 1290
Size: 492 298 2445
Fragments arranged by size:
7589 2445 1753 1432 942 879 492 476
437 308 298
BsaI GGTCTCn'nnnn_
Cuts at: 1783 4910 5389 7748 7969 8532 8542 9169 10415
Size: 3127 479 2359 221 563 10 627 1246
Cuts at: 10415 11485 16755 1783
Size: 1070 5270 2079
Fragments arranged by size:
5270 3127 2359 2079 1246 1070 627 563
479 221 10
BsaAI yAC'GTr
Cuts at: 2136 6954 8298 8862 12770 13632 13814 14404 2136
Size: 4818 1344 564 3908 862 182 590 4783
Fragments arranged by size:
4818 4783 3908 1344 862 590 564 182
BsaBI GATnn'nnATC
Cuts at: 6 899 7028 9029 9616 14565 16602 6
Size: 893 6129 2001 587 4949 2037 455
Fragments arranged by size:
6129 4949 2037 2001 893 587 455
BsaHI Gr'CG_yC
Cuts at: 250 368 1518 2843 10190 10373 10958 13057 13224
Size: 118 1150 1325 7347 183 585 2099 167
Cuts at: 13224 13607 13757 14482 14730 15432 16928 250
Size: 383 150 725 248 702 1496 373
Fragments arranged by size:
7347 2099 1496 1325 1150 725 702 585
383 373 248 183 167 150 118
BsaWI w'CCGG_w
Cuts at: 714 2357 6215 10526 12810 14240 14761 15439 15713
Size: 1643 3858 4311 2284 1430 521 678 274
Cuts at: 15713 15860 16271 714
Size: 147 411 1494
Fragments arranged by size:
4311 3858 2284 1643 1494 1430 678 521
411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2916 2946 3077 3107 5141 5146 5171 5176 6686
Size: 30 131 30 2034 5 25 5 1510
Cuts at: 6686 6716 10015 10045 11482 11512 11572 11602 11983
Size: 30 3299 30 1437 30 60 30 381
Cuts at: 11983 12013 12427 12457 12907 12937 2916
Size: 30 414 30 450 30 7030
Fragments arranged by size:
7030 3299 2034 1510 1437 450 414 381
131 60 30 30 30 30 30 30
30 30 30 25 5 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 292 2509 5167 6013 6661 7339 8644 9265 9706
Size: 2217 2658 846 648 678 1305 621 441
Cuts at: 9706 10192 11476 13030 13732 292
Size: 486 1284 1554 702 3611
Fragments arranged by size:
3611 2658 2217 1554 1305 1284 846 702
678 648 621 486 441
BseYI C'CCAG_C
Cuts at: 1925 2123 4574 4673 4802 5348 5805 6023 7647
Size: 198 2451 99 129 546 457 218 1624
Cuts at: 7647 10089 10618 11501 13068 13880 14476 15234 15811
Size: 2442 529 883 1567 812 596 758 577
Cuts at: 15811 16307 1925
Size: 496 2669
Fragments arranged by size:
2669 2451 2442 1624 1567 883 812 758
596 577 546 529 496 457 218 198
129 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 2245 2563 2761 3060 3646 4084 4747 4790
Size: 2055 318 198 299 586 438 663 43
Cuts at: 4790 6052 6425 7477 12815 13045 13231 13255 13973
Size: 1262 373 1052 5338 230 186 24 718
Cuts at: 13973 190
Size: 3268
Fragments arranged by size:
5338 3268 2055 1262 1052 718 663 586
438 373 318 299 230 198 186 43
24
BsiEI CG_ry'CG
Cuts at: 104 149 167 257 6243 7676 12105 14593 14639
Size: 45 18 90 5986 1433 4429 2488 46
Cuts at: 14639 15847 104
Size: 1208 1308
Fragments arranged by size:
5986 4429 2488 1433 1308 1208 90 46
45 18
BsiHKAI G_wGCw'C
Cuts at: 3674 7219 7424 7442 8174 9257 10534 10986 12241
Size: 3545 205 18 732 1083 1277 452 1255
Cuts at: 12241 13724 14041 14065 14843 15033 15825 3674
Size: 1483 317 24 778 190 792 4900
Fragments arranged by size:
4900 3545 1483 1277 1255 1083 792 778
732 452 317 205 190 24 18
BsmI GAATG_Cn'
Cuts at: 457 555 1842 3174 9585 9810 9993 12447 12596
Size: 98 1287 1332 6411 225 183 2454 149
Cuts at: 12596 457
Size: 4912
Fragments arranged by size:
6411 4912 2454 1332 1287 225 183 149
98
BsmBI CGTCTCn'nnnn_
Cuts at: 4623 6529 7774 8605 9042 9170 11458 12609 4623
Size: 1906 1245 831 437 128 2288 1151 9065
Fragments arranged by size:
9065 2288 1906 1245 1151 831 437 128
Bsp1286I G_dGCh'C
Cuts at: 119 991 1239 1300 1735 3674 6136 6782 6944
Size: 872 248 61 435 1939 2462 646 162
Cuts at: 6944 7219 7424 7442 7477 8174 8329 8586 9219
Size: 275 205 18 35 697 155 257 633
Cuts at: 9219 9257 9722 10340 10534 10986 11791 11804 12020
Size: 38 465 618 194 452 805 13 216
Cuts at: 12020 12205 12241 12639 13724 14041 14065 14676 14769
Size: 185 36 398 1085 317 24 611 93
Cuts at: 14769 14843 15033 15095 15825 119
Size: 74 190 62 730 1345
Fragments arranged by size:
2462 1939 1345 1085 872 805 730 697
646 633 618 611 465 452 435 398
317 275 257 248 216 205 194 190
185 162 155 93 74 62 61 38
36 35 24 18 13
BspEI T'CCGG_A
Cuts at: 714 2357 6215 16271 714
Size: 1643 3858 10056 1494
Fragments arranged by size:
10056 3858 1643 1494
BspHI T'CATG_A
Cuts at: 2985 4355 16227 2985
Size: 1370 11872 3809
Fragments arranged by size:
11872 3809 1370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 215 315 2488 2868 3801 3830 3884 4056 4793
Size: 100 2173 380 933 29 54 172 737
Cuts at: 4793 4934 5835 6018 9975 12051 12371 12749 14101
Size: 141 901 183 3957 2076 320 378 1352
Cuts at: 14101 14315 14617 14998 215
Size: 214 302 381 2268
Fragments arranged by size:
3957 2268 2173 2076 1352 933 901 737
381 380 378 320 302 214 183 172
141 100 54 29
BsrBI CCG'CTC
Cuts at: 50 1804 2499 4974 6003 8412 10124 11517 12180
Size: 1754 695 2475 1029 2409 1712 1393 663
Cuts at: 12180 14019 15343 15397 16881 17046 50
Size: 1839 1324 54 1484 165 55
Fragments arranged by size:
2475 2409 1839 1754 1712 1484 1393 1324
1029 695 663 165 55 54
BsrDI GCAATG_nn'
Cuts at: 576 2564 3687 5463 5933 6417 8711 10353 10613
Size: 1988 1123 1776 470 484 2294 1642 260
Cuts at: 10613 10646 14963 576
Size: 33 4317 2664
Fragments arranged by size:
4317 2664 2294 1988 1776 1642 1123 484
470 260 33
BsrFI r'CCGG_y
Cuts at: 252 6179 6242 7625 13519 15049 15230 15439 252
Size: 5927 63 1383 5894 1530 181 209 1864
Fragments arranged by size:
5927 5894 1864 1530 1383 209 181 63
BsrGI T'GTAC_A
Cuts at: 2604 3129 7850 8723 2604
Size: 525 4721 873 10932
Fragments arranged by size:
10932 4721 873 525
BssSI C'ACGA_G
Cuts at: 9247 9670 15322 15680 9247
Size: 423 5652 358 10618
Fragments arranged by size:
10618 5652 423 358
BstAPI GCAn_nnn'nTGC
Cuts at: 4788 5448 9702 12015 12453 14428 4788
Size: 660 4254 2313 438 1975 7411
Fragments arranged by size:
7411 4254 2313 1975 660 438
BstBI TT'CG_AA
Cuts at: 13979 15412 13979
Size: 1433 15618
BstEII G'GTnAC_C
Cuts at: 5466 7404 10996 11445 13920 5466
Size: 1938 3592 449 2475 8597
Fragments arranged by size:
8597 3592 2475 1938 449
BstXI CCAn_nnnn'nTGG
Cuts at: 453 1155 3728 5875 7401 8233 10404 10950 11766
Size: 702 2573 2147 1526 832 2171 546 816
Cuts at: 11766 12707 453
Size: 941 4797
Fragments arranged by size:
4797 2573 2171 2147 1526 941 832 816
702 546
BstYI r'GATC_y
Cuts at: 1 863 900 1205 2198 3101 3330 3542 4937
Size: 862 37 305 993 903 229 212 1395
Cuts at: 4937 5936 7029 7349 8345 9030 9447 9948 10118
Size: 999 1093 320 996 685 417 501 170
Cuts at: 10118 11154 11178 11876 12818 13326 13481 13998 14076
Size: 1036 24 698 942 508 155 517 78
Cuts at: 14076 14543 14566 14901 15147 16159 16245 16257 16603
Size: 467 23 335 246 1012 86 12 346
Cuts at: 16603 1
Size: 449
Fragments arranged by size:
1395 1093 1036 1012 999 996 993 942
903 862 698 685 517 508 501 467
449 417 346 335 320 305 246 229
212 170 155 86 78 37 24 23
12
Bsu36I CC'TnA_GG
Cuts at: 1558 13658 1558
Size: 12100 4951
BtgI C'CryG_G
Cuts at: 1148 1899 3854 4569 7747 8423 10034 11549 11792
Size: 751 1955 715 3178 676 1611 1515 243
Cuts at: 11792 12116 12488 12823 13310 13970 1148
Size: 324 372 335 487 660 4229
Fragments arranged by size:
4229 3178 1955 1611 1515 751 715 676
660 487 372 335 324 243
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6350 7037 10422 15001 15182 15345 6350
Size: 687 3385 4579 181 163 8056
Fragments arranged by size:
8056 4579 3385 687 181 163
BtsI GCAGTG_nn'
Cuts at: 581 1237 2765 3611 4056 4773 6040 6049 6287
Size: 656 1528 846 445 717 1267 9 238
Cuts at: 6287 7688 8414 9208 10532 13033 13243 13670 14294
Size: 1401 726 794 1324 2501 210 427 624
Cuts at: 14294 581
Size: 3338
Fragments arranged by size:
3338 2501 1528 1401 1324 1267 846 794
726 717 656 624 445 427 238 210
9
ClaI AT'CG_AT
Cuts at: 4136 7712 4136
Size: 3576 13475
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2937 2972 6536 6571 2937
Size: 35 3564 35 13417
Fragments arranged by size:
13417 3564 35 35
DraI TTT'AAA
Cuts at: 5166 6327 14050 5166
Size: 1161 7723 8167
Fragments arranged by size:
8167 7723 1161
DraIII CAC_nnn'GTG
Cuts at: 336 529 5448 6055 7791 8497 9300 10020 12198
Size: 193 4919 607 1736 706 803 720 2178
Cuts at: 12198 13114 336
Size: 916 4273
Fragments arranged by size:
4919 4273 2178 1736 916 803 720 706
607 193
DrdI GACnn_nn'nnGTC
Cuts at: 325 2686 5743 14757 15615 325
Size: 2361 3057 9014 858 1761
Fragments arranged by size:
9014 3057 2361 1761 858
EaeI y'GGCC_r
Cuts at: 146 164 254 3587 3684 4401 4622 5246 5865
Size: 18 90 3333 97 717 221 624 619
Cuts at: 5865 6143 6224 7166 7244 7862 9143 9614 10037
Size: 278 81 942 78 618 1281 471 423
Cuts at: 10037 12057 12113 12785 14636 14810 15201 15228 16573
Size: 2020 56 672 1851 174 391 27 1345
Cuts at: 16573 146
Size: 624
Fragments arranged by size:
3333 2020 1851 1345 1281 942 717 672
624 624 619 618 471 423 391 278
221 174 97 90 81 78 56 27
18
EagI C'GGCC_G
Cuts at: 146 164 254 14636 146
Size: 18 90 14382 2561
Fragments arranged by size:
14382 2561 90 18
EarI CTCTTCn'nnn_
Cuts at: 277 1753 1967 3029 3146 3203 3923 4049 4591
Size: 1476 214 1062 117 57 720 126 542
Cuts at: 4591 4652 6354 6382 6412 6583 6790 6949 7486
Size: 61 1702 28 30 171 207 159 537
Cuts at: 7486 7927 8608 8762 9572 10540 10544 11422 11701
Size: 441 681 154 810 968 4 878 279
Cuts at: 11701 12484 15074 15284 277
Size: 783 2590 210 2044
Fragments arranged by size:
2590 2044 1702 1476 1062 968 878 810
783 720 681 542 537 441 279 214
210 207 171 159 154 126 117 61
57 30 28 4
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 196 5432 5538 7822 11917 15259 15569 15715 16861
Size: 5236 106 2284 4095 3342 310 146 1146
Cuts at: 16861 196
Size: 386
Fragments arranged by size:
5236 4095 3342 2284 1146 386 310 146
106
EcoICRI GAG'CTC
Cuts at: 3672 9255 13722 14063 3672
Size: 5583 4467 341 6660
Fragments arranged by size:
6660 5583 4467 341
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 283 295 313 632 869 1381 1465 1848
Size: 30 12 18 319 237 512 84 383
Cuts at: 1848 2173 2918 3256 3263 3922 3997 5105 6016
Size: 325 745 338 7 659 75 1108 911
Cuts at: 6016 6314 6564 6626 6673 6715 6841 7456 7585
Size: 298 250 62 47 42 126 615 129
Cuts at: 7585 7637 7908 7959 7973 8023 8236 8566 8692
Size: 52 271 51 14 50 213 330 126
Cuts at: 8692 8818 9142 9472 9709 9814 10090 10564 10947
Size: 126 324 330 237 105 276 474 383
Cuts at: 10947 11437 11446 11578 11665 11803 12048 12415 12454
Size: 490 9 132 87 138 245 367 39
Cuts at: 12454 12934 13159 13363 13536 13735 14876 15308 16055
Size: 480 225 204 173 199 1141 432 747
Cuts at: 16055 16323 253
Size: 268 981
Fragments arranged by size:
1141 1108 981 911 747 745 659 615
512 490 480 474 432 383 383 367
338 330 330 325 324 319 298 276
271 268 250 245 237 237 225 213
204 199 173 138 132 129 126 126
126 105 87 84 75 62 52 51
50 47 42 39 30 18 14 12
9 7
EcoNI CCTnn'n_nnAGG
Cuts at: 908 2039 2116 5107 5953 6643 6820 8561 10021
Size: 1131 77 2991 846 690 177 1741 1460
Cuts at: 10021 11698 12085 908
Size: 1677 387 5874
Fragments arranged by size:
5874 2991 1741 1677 1460 1131 846 690
387 177 77
EcoO109I rG'GnC_Cy
Cuts at: 516 1554 1602 2547 4089 5576 6778 6779 8132
Size: 1038 48 945 1542 1487 1202 1 1353
Cuts at: 8132 8326 11691 11883 12276 13395 14190 516
Size: 194 3365 192 393 1119 795 3377
Fragments arranged by size:
3377 3365 1542 1487 1353 1202 1119 1038
945 795 393 194 192 48 1
EcoRI G'AATT_C
Cuts at: 397 3529 9430 10900 11818 14055 397
Size: 3132 5901 1470 918 2237 3393
Fragments arranged by size:
5901 3393 3132 2237 1470 918
EcoRV GAT'ATC
Cuts at: 1014 4776 7585 1014
Size: 3762 2809 10480
Fragments arranged by size:
10480 3762 2809
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1696 2128 3474 3952 4578 5035 8836 9967 10607
Size: 432 1346 478 626 457 3801 1131 640
Cuts at: 10607 11712 15070 1696
Size: 1105 3358 3677
Fragments arranged by size:
3801 3677 3358 1346 1131 1105 640 626
478 457 432
FspI TGC'GCA
Cuts at: 211 304 7255 13453 14832 211
Size: 93 6951 6198 1379 2430
Fragments arranged by size:
6951 6198 2430 1379 93
HaeII r_GCGC'y
Cuts at: 253 274 290 351 1035 1177 2613 3693 4018
Size: 21 16 61 684 142 1436 1080 325
Cuts at: 4018 7002 7104 7417 7572 7931 8536 10069 11673
Size: 2984 102 313 155 359 605 1533 1604
Cuts at: 11673 12921 13227 13610 13760 14485 14733 15755 16734
Size: 1248 306 383 150 725 248 1022 979
Cuts at: 16734 253
Size: 570
Fragments arranged by size:
2984 1604 1533 1436 1248 1080 1022 979
725 684 605 570 383 359 325 313
306 248 155 150 142 102 61 21
16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 892 924 1126 1158 1403 1435 1529 1561 1788
Size: 32 202 32 245 32 94 32 227
Cuts at: 1788 1820 1942 1974 3093 3125 4686 4718 4963
Size: 32 122 32 1119 32 1561 32 245
Cuts at: 4963 4995 6070 6102 6413 6445 7665 7697 7998
Size: 32 1075 32 311 32 1220 32 301
Cuts at: 7998 8030 8768 8800 10015 10047 11075 11107 11275
Size: 32 738 32 1215 32 1028 32 168
Cuts at: 11275 11307 11482 11514 11983 12015 12467 12499 12730
Size: 32 175 32 469 32 452 32 231
Cuts at: 12730 12762 13198 13230 13473 13505 14893 14923 14925
Size: 32 436 32 243 32 1388 30 2
Cuts at: 14925 14955 892
Size: 30 2988
Fragments arranged by size:
2988 1561 1388 1220 1215 1119 1075 1028
738 469 452 436 311 301 245 245
243 231 227 202 175 168 122 94
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 855 2319 2808 2916 4419 8890 9744 10164 12972
Size: 1464 489 108 1503 4471 854 420 2808
Cuts at: 12972 13737 14090 855
Size: 765 353 3816
Fragments arranged by size:
4471 3816 2808 1503 1464 854 765 489
420 353 108
HindIII A'AGCT_T
Cuts at: 2812 4180 4221 2812
Size: 1368 41 15642
Fragments arranged by size:
15642 1368 41
HpaI GTT'AAC
Cuts at: 855 2319 855
Size: 1464 15587
KasI G'GCGC_C
Cuts at: 249 13223 13606 13756 14481 14729 249
Size: 12974 383 150 725 248 2571
Fragments arranged by size:
12974 2571 725 383 248 150
KpnI G_GTAC'C
Cuts at: 3120 14071 3120
Size: 10951 6100
MfeI C'AATT_G
Cuts at: 3029 5303 10295 12311 3029
Size: 2274 4992 2016 7769
Fragments arranged by size:
7769 4992 2274 2016
MluI A'CGCG_T
Cuts at: 16484 16484
Size: 17051
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 92 3545 6089 6572 7418 8620 11182 12498 13133
Size: 3453 2544 483 846 1202 2562 1316 635
Cuts at: 13133 13241 13286 13571 15722 15906 92
Size: 108 45 285 2151 184 1237
Fragments arranged by size:
3453 2562 2544 2151 1316 1237 1202 846
635 483 285 184 108 45
MscI TGG'CCA
Cuts at: 3686 4403 5248 5867 6145 6226 7168 7246 9145
Size: 717 845 619 278 81 942 78 1899
Cuts at: 9145 9616 10039 12059 12115 14812 3686
Size: 471 423 2020 56 2697 5925
Fragments arranged by size:
5925 2697 2020 1899 942 845 717 619
471 423 278 81 78 56
MslI CAynn'nnrTG
Cuts at: 1898 2021 3622 6157 6623 6952 7222 7675 7954
Size: 123 1601 2535 466 329 270 453 279
Cuts at: 7954 9193 10132 11476 12208 12541 12612 12705 12991
Size: 1239 939 1344 732 333 71 93 286
Cuts at: 12991 13630 15040 15167 1898
Size: 639 1410 127 3782
Fragments arranged by size:
3782 2535 1601 1410 1344 1239 939 732
639 466 453 333 329 286 279 270
127 123 93 71
MspA1I CmG'CkG
Cuts at: 225 860 1929 2079 4215 4673 4806 5768 6573
Size: 635 1069 150 2136 458 133 962 805
Cuts at: 6573 7266 7293 7434 7563 7733 8832 9545 9897
Size: 693 27 141 129 170 1099 713 352
Cuts at: 9897 10507 10639 10869 11340 12367 12432 13572 13594
Size: 610 132 230 471 1027 65 1140 22
Cuts at: 13594 13706 13875 14162 14476 14836 15849 16094 225
Size: 112 169 287 314 360 1013 245 1182
Fragments arranged by size:
2136 1182 1140 1099 1069 1027 1013 962
805 713 693 635 610 471 458 360
352 314 287 245 230 170 169 150
141 133 132 129 112 65 27 22
NaeI GCC'GGC
Cuts at: 254 7627 13521 15232 254
Size: 7373 5894 1711 2073
Fragments arranged by size:
7373 5894 2073 1711
NarI GG'CG_CC
Cuts at: 250 13224 13607 13757 14482 14730 250
Size: 12974 383 150 725 248 2571
Fragments arranged by size:
12974 2571 725 383 248 150
NcoI C'CATG_G
Cuts at: 8423 10034 11549 12116 12488 8423
Size: 1611 1515 567 372 12986
Fragments arranged by size:
12986 1611 1515 567 372
NdeI CA'TA_TG
Cuts at: 2601 6684 2601
Size: 4083 12968
NgoMIV G'CCGG_C
Cuts at: 252 7625 13519 15230 252
Size: 7373 5894 1711 2073
Fragments arranged by size:
7373 5894 2073 1711
NheI G'CTAG_C
Cuts at: 9588 9588
Size: 17051
NruI TCG'CGA
Cuts at: 1913 1913
Size: 17051
NsiI A_TGCA'T
Cuts at: 1316 1316
Size: 17051
NspI r_CATG'y
Cuts at: 1318 1355 9486 10224 10323 11733 12129 12271 12466
Size: 37 8131 738 99 1410 396 142 195
Cuts at: 12466 12579 12965 14104 14604 15511 1318
Size: 113 386 1139 500 907 2858
Fragments arranged by size:
8131 2858 1410 1139 907 738 500 396
386 195 142 113 99 37
PasI CC'CwG_GG
Cuts at: 4157 12415 4157
Size: 8258 8793
PciI A'CATG_T
Cuts at: 10220 10319 12125 12267 15507 10220
Size: 99 1806 142 3240 11764
Fragments arranged by size:
11764 3240 1806 142 99
PflMI CCAn_nnn'nTGG
Cuts at: 3564 5355 5928 6929 7604 9636 9792 10109 11019
Size: 1791 573 1001 675 2032 156 317 910
Cuts at: 11019 12198 13706 3564
Size: 1179 1508 6909
Fragments arranged by size:
6909 2032 1791 1508 1179 1001 910 675
573 317 156
PfoI T'CCnGG_A
Cuts at: 971 3839 9119 9887 10949 11012 11603 12911 971
Size: 2868 5280 768 1062 63 591 1308 5111
Fragments arranged by size:
5280 5111 2868 1308 1062 768 591 63
PmeI GTTT'AAAC
Cuts at: 14050 14050
Size: 17051
PmlI CAC'GTG
Cuts at: 2136 6954 8298 8862 12770 13632 13814 2136
Size: 4818 1344 564 3908 862 182 5373
Fragments arranged by size:
5373 4818 3908 1344 862 564 182
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7039 7071 9304 9336 12907 12939 16190 16222 7039
Size: 32 2233 32 3571 32 3251 32 7868
Fragments arranged by size:
7868 3571 3251 2233 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 516 1554 1602 4089 5576 8132 11691 12276 13395
Size: 1038 48 2487 1487 2556 3559 585 1119
Cuts at: 13395 516
Size: 4172
Fragments arranged by size:
4172 3559 2556 2487 1487 1119 1038 585
48
PshAI GACnn'nnGTC
Cuts at: 1067 6371 12893 1067
Size: 5304 6522 5225
Fragments arranged by size:
6522 5304 5225
PsiI TTA'TAA
Cuts at: 9085 11683 9085
Size: 2598 14453
PspOMI G'GGCC_C
Cuts at: 6778 8325 6778
Size: 1547 15504
PspXI vC'TCGA_Gb
Cuts at: 6838 11723 6838
Size: 4885 12166
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 6154 6186 6154
Size: 32 17019
PstI C_TGCA'G
Cuts at: 200 1487 1653 6995 7349 10176 10358 11442 11533
Size: 1287 166 5342 354 2827 182 1084 91
Cuts at: 11533 12330 12458 13577 14098 14783 200
Size: 797 128 1119 521 685 2468
Fragments arranged by size:
5342 2827 2468 1287 1119 1084 797 685
521 354 182 166 128 91
PvuI CG_AT'CG
Cuts at: 104 12105 104
Size: 12001 5050
PvuII CAG'CTG
Cuts at: 1929 2079 4215 4673 7434 7563 9545 9897 10507
Size: 150 2136 458 2761 129 1982 352 610
Cuts at: 10507 10639 11340 12432 13572 13594 13706 13875 14476
Size: 132 701 1092 1140 22 112 169 601
Cuts at: 14476 14836 1929
Size: 360 4144
Fragments arranged by size:
4144 2761 2136 1982 1140 1092 701 610
601 458 360 352 169 150 132 129
112 22
RsrII CG'GwC_CG
Cuts at: 15246 15246
Size: 17051
SacI G_AGCT'C
Cuts at: 3674 9257 13724 14065 3674
Size: 5583 4467 341 6660
Fragments arranged by size:
6660 5583 4467 341
SalI G'TCGA_C
Cuts at: 9742 12970 14088 9742
Size: 3228 1118 12705
Fragments arranged by size:
12705 3228 1118
SanDI GG'GwC_CC
Cuts at: 8132 8132
Size: 17051
SapI GCTCTTCn'nnn_
Cuts at: 277 4049 6583 6790 6949 7486 10540 10544 15074
Size: 3772 2534 207 159 537 3054 4 4530
Cuts at: 15074 15284 277
Size: 210 2044
Fragments arranged by size:
4530 3772 3054 2534 2044 537 210 207
159 4
SbfI CC_TGCA'GG
Cuts at: 14098 14098
Size: 17051
ScaI AGT'ACT
Cuts at: 2274 11255 11587 16568 2274
Size: 8981 332 4981 2757
Fragments arranged by size:
8981 4981 2757 332
SexAI A'CCwGG_T
Cuts at: 3119 9746 10016 10699 3119
Size: 6627 270 683 9471
Fragments arranged by size:
9471 6627 683 270
SfcI C'TryA_G
Cuts at: 33 196 1483 1649 6991 7345 10172 10354 10714
Size: 163 1287 166 5342 354 2827 182 360
Cuts at: 10714 11438 11529 12326 12454 13573 14094 14779 15772
Size: 724 91 797 128 1119 521 685 993
Cuts at: 15772 15963 33
Size: 191 1121
Fragments arranged by size:
5342 2827 1287 1121 1119 993 797 724
685 521 360 354 191 182 166 163
128 91
SfoI GGC'GCC
Cuts at: 251 13225 13608 13758 14483 14731 251
Size: 12974 383 150 725 248 2571
Fragments arranged by size:
12974 2571 725 383 248 150
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 17051
SmaI CCC'GGG
Cuts at: 120 8324 11152 11191 14073 120
Size: 8204 2828 39 2882 3098
Fragments arranged by size:
8204 3098 2882 2828 39
SmlI C'TyrA_G
Cuts at: 1269 2305 6838 8894 9331 9771 11560 11723 11832
Size: 1036 4533 2056 437 440 1789 163 109
Cuts at: 11832 13300 14206 15121 15613 15875 1269
Size: 1468 906 915 492 262 2445
Fragments arranged by size:
4533 2445 2056 1789 1468 1036 915 906
492 440 437 262 163 109
SpeI A'CTAG_T
Cuts at: 67 67
Size: 17051
SphI G_CATG'C
Cuts at: 1355 11733 12466 12965 14104 14604 1355
Size: 10378 733 499 1139 500 3802
Fragments arranged by size:
10378 3802 1139 733 500 499
SspI AAT'ATT
Cuts at: 705 2837 8882 705
Size: 2132 6045 8874
Fragments arranged by size:
8874 6045 2132
StuI AGG'CCT
Cuts at: 1890 7501 8567 9230 1890
Size: 5611 1066 663 9711
Fragments arranged by size:
9711 5611 1066 663
StyI C'CwwG_G
Cuts at: 2112 2696 4007 4398 5792 6875 7163 7409 7757
Size: 584 1311 391 1394 1083 288 246 348
Cuts at: 7757 8423 10034 10376 11549 12116 12144 12488 12503
Size: 666 1611 342 1173 567 28 344 15
Cuts at: 12503 12788 13610 14185 14539 2112
Size: 285 822 575 354 4624
Fragments arranged by size:
4624 1611 1394 1311 1173 1083 822 666
584 575 567 391 354 348 344 342
288 285 246 28 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3115 4993 5716 5950 6361 6763 7131 7639 8196
Size: 1878 723 234 411 402 368 508 557
Cuts at: 8196 8476 8502 12066 12375 13020 13066 13530 13630
Size: 280 26 3564 309 645 46 464 100
Cuts at: 13630 14634 14767 15226 15435 3115
Size: 1004 133 459 209 4731
Fragments arranged by size:
4731 3564 1878 1004 723 645 557 508
464 459 411 402 368 309 280 234
209 133 100 46 26
TatI w'GTAC_w
Cuts at: 2272 2604 3084 3129 4167 4452 4896 6269 7850
Size: 332 480 45 1038 285 444 1373 1581
Cuts at: 7850 8027 8723 9005 11253 11585 12644 16566 2272
Size: 177 696 282 2248 332 1059 3922 2757
Fragments arranged by size:
3922 2757 2248 1581 1373 1059 1038 696
480 444 332 332 285 282 177 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 143 175 1206 1238 2770 2802 2916 2948 6266
Size: 32 1031 32 1532 32 114 32 3318
Cuts at: 6266 6298 7021 7053 8270 8302 11139 11171 16568
Size: 32 723 32 1217 32 2837 32 5397
Cuts at: 16568 16600 143
Size: 32 594
Fragments arranged by size:
5397 3318 2837 1532 1217 1031 723 594
114 32 32 32 32 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1417 5491 7828 11473 11496 14848 1417
Size: 4074 2337 3645 23 3352 3620
Fragments arranged by size:
4074 3645 3620 3352 2337 23
XbaI T'CTAG_A
Cuts at: 14082 14082
Size: 17051
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2514 4014 6757 12859 13113 13686 14546 2514
Size: 1500 2743 6102 254 573 860 5019
Fragments arranged by size:
6102 5019 2743 1500 860 573 254
XhoI C'TCGA_G
Cuts at: 6838 11723 6838
Size: 4885 12166
XmaI C'CCGG_G
Cuts at: 118 8322 11150 11189 14071 118
Size: 8204 2828 39 2882 3098
Fragments arranged by size:
8204 3098 2882 2828 39
XmnI GAAnn'nnTTC
Cuts at: 1120 1364 4222 5065 6211 6725 8677 10690 10852
Size: 244 2858 843 1146 514 1952 2013 162
Cuts at: 10852 1120
Size: 7319
Fragments arranged by size:
7319 2858 2013 1952 1146 843 514 244
162
ZraI GAC'GTC
Cuts at: 369 369
Size: 17051
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NruI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BsiWI BssHII BstZ17I FseI FspAI
NotI PacI SacII SfiI SgrAI SnaBI SrfI SwaI