Restriction map for
pF1KA0327
(Circular) (Six-base) MAPSORT of: pF1KA0327.seq Check: 8773 from: 1 to: 5573
pF1KA0327 5573 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 2153 2611 2153
Size: 458 5115
Acc65I G'GTAC_C
Cuts at: 2589 2589
Size: 5573
AclI AA'CG_TT
Cuts at: 5413 5413
Size: 5573
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2161 3398 3830 4577 4845 2161
Size: 1237 432 747 268 2889
Fragments arranged by size:
2889 1237 747 432 268
AflIII A'CryG_T
Cuts at: 1264 1742 2925 4029 5006 1264
Size: 478 1183 1104 977 1831
Fragments arranged by size:
1831 1183 1104 977 478
AgeI A'CCGG_T
Cuts at: 1639 3961 1639
Size: 2322 3251
AleI CACnn'nnGTG
Cuts at: 1828 1828
Size: 5573
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 347 904 347
Size: 557 5016
AlwNI CAG_nnn'CTG
Cuts at: 577 1698 2200 2878 4445 577
Size: 1121 502 678 1567 1705
Fragments arranged by size:
1705 1567 1121 678 502
ApaLI G'TGCA_C
Cuts at: 4343 4343
Size: 5573
ApoI r'AATT_y
Cuts at: 502 946 1182 2577 4926 5182 502
Size: 444 236 1395 2349 256 893
Fragments arranged by size:
2349 1395 893 444 256 236
AseI AT'TA_AT
Cuts at: 20 959 20
Size: 939 4634
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5573
AvaI C'yCGr_G
Cuts at: 1192 1786 1899 2593 1192
Size: 594 113 694 4172
Fragments arranged by size:
4172 694 594 113
BamHI G'GATC_C
Cuts at: 2598 2598
Size: 5573
BanI G'GyrC_C
Cuts at: 1602 1839 2589 3003 3251 3286 1602
Size: 237 750 414 248 35 3889
Fragments arranged by size:
3889 750 414 248 237 35
BanII G_rGCy'C
Cuts at: 392 406 2001 2249 2587 3617 392
Size: 14 1595 248 338 1030 2348
Fragments arranged by size:
2348 1595 1030 338 248 14
BbeI G_GCGC'C
Cuts at: 1606 1843 3007 3255 1606
Size: 237 1164 248 3924
Fragments arranged by size:
3924 1164 248 237
BbsI GAAGACnn'nnnn_
Cuts at: 2208 2208
Size: 5573
BbvCI CC'TCA_GC
Cuts at: 1732 2254 1732
Size: 522 5051
BciVI GTATCCnnnnn_n'
Cuts at: 801 3471 4232 5573 801
Size: 2670 761 1341 801
Fragments arranged by size:
2670 1341 801 761
BclI T'GATC_A
Cuts at: 359 1148 2520 3093 359
Size: 789 1372 573 2839
Fragments arranged by size:
2839 1372 789 573
BglII A'GATC_T
Cuts at: 516 1383 3088 5125 516
Size: 867 1705 2037 964
Fragments arranged by size:
2037 1705 964 867
BlpI GC'TnA_GC
Cuts at: 622 2685 622
Size: 2063 3510
Bme1580I G_kGCm'C
Cuts at: 1086 3198 3291 4347 1086
Size: 2112 93 1056 2312
Fragments arranged by size:
2312 2112 1056 93
BmgBI CAC'GTC
Cuts at: 651 651
Size: 5573
BmrI ACTGGGnnnn_n'
Cuts at: 1010 1165 2785 2866 3201 5345 1010
Size: 155 1620 81 335 2144 1238
Fragments arranged by size:
2144 1620 1238 335 155 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2035 3940 2035
Size: 1905 3668
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 294 572 592 680 715 2266 294
Size: 278 20 88 35 1551 3601
Fragments arranged by size:
3601 1551 278 88 35 20
Bpu10I CC'TnA_GC
Cuts at: 1732 2248 2254 1732
Size: 516 6 5051
Fragments arranged by size:
5051 516 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 883 1233 1918 2005 2749 3628 4120 4418 883
Size: 350 685 87 744 879 492 298 2038
Fragments arranged by size:
2038 879 744 685 492 350 298 87
BsaI GGTCTCn'nnnn_
Cuts at: 1679 5277 1679
Size: 3598 1975
BsaAI yAC'GTr
Cuts at: 795 1267 1431 2926 795
Size: 472 164 1495 3442
Fragments arranged by size:
3442 1495 472 164
BsaBI GATnn'nnATC
Cuts at: 6 3087 5124 6
Size: 3081 2037 455
Fragments arranged by size:
3081 2037 455
BsaHI Gr'CG_yC
Cuts at: 1603 1840 3004 3252 3954 5450 1603
Size: 237 1164 248 702 1496 1726
Fragments arranged by size:
1726 1496 1164 702 248 237
BsaWI w'CCGG_w
Cuts at: 950 1639 2290 2762 3283 3961 4235 4382 4793
Size: 689 651 472 521 678 274 147 411
Cuts at: 4793 950
Size: 1730
Fragments arranged by size:
1730 689 678 651 521 472 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 217 247 625 655 2264 2294 217
Size: 30 378 30 1609 30 3496
Fragments arranged by size:
3496 1609 378 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 400 1523 2353 400
Size: 1123 830 3620
Fragments arranged by size:
3620 1123 830
BseYI C'CCAG_C
Cuts at: 165 2420 2998 3756 4333 4829 165
Size: 2255 578 758 577 496 909
Fragments arranged by size:
2255 909 758 578 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 673 1411 1517 1954 673
Size: 738 106 437 4292
Fragments arranged by size:
4292 738 437 106
BsiEI CG_ry'CG
Cuts at: 104 2153 3115 3161 4369 104
Size: 2049 962 46 1208 1308
Fragments arranged by size:
2049 1308 1208 962 46
BsiHKAI G_wGCw'C
Cuts at: 392 558 2587 3365 3555 4347 392
Size: 166 2029 778 190 792 1618
Fragments arranged by size:
2029 1618 792 778 190 166
BsmI GAATG_Cn'
Cuts at: 1563 1563
Size: 5573
BsmBI CGTCTCn'nnnn_
Cuts at: 214 312 2006 214
Size: 98 1694 3781
Fragments arranged by size:
3781 1694 98
Bsp1286I G_dGCh'C
Cuts at: 392 406 558 1086 2001 2249 2587 3198 3291
Size: 14 152 528 915 248 338 611 93
Cuts at: 3291 3365 3555 3617 4347 392
Size: 74 190 62 730 1618
Fragments arranged by size:
1618 915 730 611 528 338 248 190
152 93 74 62 14
BspEI T'CCGG_A
Cuts at: 950 4793 950
Size: 3843 1730
BspHI T'CATG_A
Cuts at: 2499 4749 2499
Size: 2250 3323
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1697 2623 2837 3139 3520 1697
Size: 926 214 302 381 3750
Fragments arranged by size:
3750 926 381 302 214
BsrBI CCG'CTC
Cuts at: 50 115 1847 3865 3919 5403 5568 50
Size: 65 1732 2018 54 1484 165 55
Fragments arranged by size:
2018 1732 1484 165 65 55 54
BsrDI GCAATG_nn'
Cuts at: 3485 3485
Size: 5573
BsrFI r'CCGG_y
Cuts at: 1639 3571 3752 3961 1639
Size: 1932 181 209 3251
Fragments arranged by size:
3251 1932 209 181
BssHII G'CGCG_C
Cuts at: 158 699 158
Size: 541 5032
BssSI C'ACGA_G
Cuts at: 2032 2185 3844 4202 2032
Size: 153 1659 358 3403
Fragments arranged by size:
3403 1659 358 153
BstAPI GCAn_nnn'nTGC
Cuts at: 2200 2950 2200
Size: 750 4823
BstBI TT'CG_AA
Cuts at: 3934 3934
Size: 5573
BstEII G'GTnAC_C
Cuts at: 1869 1869
Size: 5573
BstXI CCAn_nnnn'nTGG
Cuts at: 261 1234 1830 1881 2053 261
Size: 973 596 51 172 3781
Fragments arranged by size:
3781 973 596 172 51
BstYI r'GATC_y
Cuts at: 1 516 1383 1679 1790 2598 3065 3088 3423
Size: 515 867 296 111 808 467 23 335
Cuts at: 3423 3669 4681 4767 4779 5125 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 867 808 515 467 449 346 335
296 246 111 86 23 12
BtgI C'CryG_G
Cuts at: 107 365 406 2052 2096 107
Size: 258 41 1646 44 3584
Fragments arranged by size:
3584 1646 258 44 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3523 3704 3867 3523
Size: 181 163 5229
Fragments arranged by size:
5229 181 163
BtsI GCAGTG_nn'
Cuts at: 2201 2816 2201
Size: 615 4958
DraI TTT'AAA
Cuts at: 1284 2572 1284
Size: 1288 4285
DrdI GACnn_nn'nnGTC
Cuts at: 1642 3279 4137 1642
Size: 1637 858 3078
Fragments arranged by size:
3078 1637 858
EaeI y'GGCC_r
Cuts at: 110 2039 2055 3158 3332 3723 3750 5095 110
Size: 1929 16 1103 174 391 27 1345 588
Fragments arranged by size:
1929 1345 1103 588 391 174 27 16
EagI C'GGCC_G
Cuts at: 3158 3158
Size: 5573
EarI CTCTTCn'nnn_
Cuts at: 212 713 1193 1960 2395 3596 3806 212
Size: 501 480 767 435 1201 210 1979
Fragments arranged by size:
1979 1201 767 501 480 435 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2061 3781 4091 4237 5383 2061
Size: 1720 310 146 1146 2251
Fragments arranged by size:
2251 1720 1146 310 146
EcoICRI GAG'CTC
Cuts at: 390 2585 390
Size: 2195 3378
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 294 572 592 680 715 2161 2266 3398 3830
Size: 278 20 88 35 1446 105 1132 432
Cuts at: 3830 4577 4845 294
Size: 747 268 1022
Fragments arranged by size:
1446 1132 1022 747 432 278 268 105
88 35 20
EcoNI CCTnn'n_nnAGG
Cuts at: 436 1933 2281 436
Size: 1497 348 3728
Fragments arranged by size:
3728 1497 348
EcoO109I rG'GnC_Cy
Cuts at: 258 1722 2712 258
Size: 1464 990 3119
Fragments arranged by size:
3119 1464 990
EcoRI G'AATT_C
Cuts at: 2577 2577
Size: 5573
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3592 3592
Size: 5573
FspI TGC'GCA
Cuts at: 787 1987 3354 787
Size: 1200 1367 3006
Fragments arranged by size:
3006 1367 1200
FspAI rTGC'GCAy
Cuts at: 787 1987 787
Size: 1200 4373
HaeII r_GCGC'y
Cuts at: 1606 1843 2262 3007 3255 4277 5256 1606
Size: 237 419 745 248 1022 979 1923
Fragments arranged by size:
1923 1022 979 745 419 248 237
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 217 249 287 319 842 874 1216 1248 1334
Size: 32 38 32 523 32 342 32 86
Cuts at: 1334 1366 1502 1534 1882 1914 3415 3445 3447
Size: 32 136 32 348 32 1501 30 2
Cuts at: 3447 3477 217
Size: 30 2313
Fragments arranged by size:
2313 1501 523 348 342 136 86 38
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 582 2154 2483 2612 582
Size: 1572 329 129 3543
Fragments arranged by size:
3543 1572 329 129
HpaI GTT'AAC
Cuts at: 2483 2483
Size: 5573
KasI G'GCGC_C
Cuts at: 1602 1839 3003 3251 1602
Size: 237 1164 248 3924
Fragments arranged by size:
3924 1164 248 237
KpnI G_GTAC'C
Cuts at: 2593 2593
Size: 5573
MluI A'CGCG_T
Cuts at: 5006 5006
Size: 5573
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 785 4244 4428 785
Size: 3459 184 1930
Fragments arranged by size:
3459 1930 184
MscI TGG'CCA
Cuts at: 2057 3334 2057
Size: 1277 4296
MslI CAynn'nnrTG
Cuts at: 793 1114 1372 1828 3562 3689 793
Size: 321 258 456 1734 127 2677
Fragments arranged by size:
2677 1734 456 321 258 127
MspA1I CmG'CkG
Cuts at: 350 367 408 1718 2684 2998 3358 4371 4616
Size: 17 41 1310 966 314 360 1013 245
Cuts at: 4616 350
Size: 1307
Fragments arranged by size:
1310 1307 1013 966 360 314 245 41
17
NaeI GCC'GGC
Cuts at: 3754 3754
Size: 5573
NarI GG'CG_CC
Cuts at: 1603 1840 3004 3252 1603
Size: 237 1164 248 3924
Fragments arranged by size:
3924 1164 248 237
NcoI C'CATG_G
Cuts at: 107 2052 107
Size: 1945 3628
NgoMIV G'CCGG_C
Cuts at: 3752 3752
Size: 5573
NspI r_CATG'y
Cuts at: 873 1746 2442 2626 3126 4033 873
Size: 873 696 184 500 907 2413
Fragments arranged by size:
2413 907 873 696 500 184
PciI A'CATG_T
Cuts at: 1742 4029 1742
Size: 2287 3286
PfoI T'CCnGG_A
Cuts at: 549 2045 2282 549
Size: 1496 237 3840
Fragments arranged by size:
3840 1496 237
PmeI GTTT'AAAC
Cuts at: 2572 2572
Size: 5573
PmlI CAC'GTG
Cuts at: 795 1267 1431 795
Size: 472 164 4937
Fragments arranged by size:
4937 472 164
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1383 1415 4712 4744 1383
Size: 32 3297 32 2212
Fragments arranged by size:
3297 2212 32 32
PpuMI rG'GwC_Cy
Cuts at: 258 1722 258
Size: 1464 4109
PstI C_TGCA'G
Cuts at: 1598 1694 2360 2620 3305 1598
Size: 96 666 260 685 3866
Fragments arranged by size:
3866 685 666 260 96
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5573
PvuII CAG'CTG
Cuts at: 2998 3358 2998
Size: 360 5213
RsrII CG'GwC_CG
Cuts at: 3768 3768
Size: 5573
SacI G_AGCT'C
Cuts at: 392 2587 392
Size: 2195 3378
SacII CC_GC'GG
Cuts at: 368 409 368
Size: 41 5532
SalI G'TCGA_C
Cuts at: 2152 2610 2152
Size: 458 5115
SanDI GG'GwC_CC
Cuts at: 1722 1722
Size: 5573
SapI GCTCTTCn'nnn_
Cuts at: 3596 3806 3596
Size: 210 5363
SbfI CC_TGCA'GG
Cuts at: 2620 2620
Size: 5573
ScaI AGT'ACT
Cuts at: 5090 5090
Size: 5573
SexAI A'CCwGG_T
Cuts at: 734 1865 734
Size: 1131 4442
SfcI C'TryA_G
Cuts at: 33 1594 1690 2356 2616 3301 4294 4485 33
Size: 1561 96 666 260 685 993 191 1121
Fragments arranged by size:
1561 1121 993 685 666 260 191 96
SfoI GGC'GCC
Cuts at: 1604 1841 3005 3253 1604
Size: 237 1164 248 3924
Fragments arranged by size:
3924 1164 248 237
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5573
SgrAI Cr'CCGG_yG
Cuts at: 1639 1639
Size: 5573
SmaI CCC'GGG
Cuts at: 1194 2595 1194
Size: 1401 4172
SmlI C'TyrA_G
Cuts at: 862 1212 1933 2020 2728 3643 4135 4397 862
Size: 350 721 87 708 915 492 262 2038
Fragments arranged by size:
2038 915 721 708 492 350 262 87
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5573
SphI G_CATG'C
Cuts at: 2626 3126 2626
Size: 500 5073
SspI AAT'ATT
Cuts at: 423 1333 423
Size: 910 4663
StyI C'CwwG_G
Cuts at: 107 254 1874 2052 2707 3061 107
Size: 147 1620 178 655 354 2619
Fragments arranged by size:
2619 1620 655 354 178 147
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1355 1597 2139 2140 2320 3156 3289 3748 3957
Size: 242 542 1 180 836 133 459 209
Cuts at: 3957 1355
Size: 2971
Fragments arranged by size:
2971 836 542 459 242 209 180 133
1
TatI w'GTAC_w
Cuts at: 5088 5088
Size: 5573
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 625 657 2264 2296 5090 5122 625
Size: 32 1607 32 2794 32 1076
Fragments arranged by size:
2794 1607 1076 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3370 3370
Size: 5573
XbaI T'CTAG_A
Cuts at: 519 1026 2604 519
Size: 507 1578 3488
Fragments arranged by size:
3488 1578 507
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2290 3068 2290
Size: 778 4795
XmaI C'CCGG_G
Cuts at: 1192 2593 1192
Size: 1401 4172
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PmlI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmtI BsiWI BsrGI
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI HindIII
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PshAI PsiI PspOMI PspXI PsrI SfiI SnaBI
SrfI StuI SwaI XhoI XmnI ZraI