Restriction map for
pF1KA0330
(Circular) (Six-base) MAPSORT of: pF1KA0330.seq Check: 8130 from: 1 to: 9773
pF1KA0330 9773 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3006 5778 6049 6207 6310 3006
Size: 2772 271 158 103 6469
Fragments arranged by size:
6469 2772 271 158 103
AccI GT'mk_AC
Cuts at: 1331 1649 6811 1331
Size: 318 5162 4293
Fragments arranged by size:
5162 4293 318
Acc65I G'GTAC_C
Cuts at: 2990 3225 6789 2990
Size: 235 3564 5974
Fragments arranged by size:
5974 3564 235
AclI AA'CG_TT
Cuts at: 9613 9613
Size: 9773
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1987 2179 2377 2548 2621 2915 3154 3463 3943
Size: 192 198 171 73 294 239 309 480
Cuts at: 3943 4022 4067 4369 4978 4996 6134 6643 7598
Size: 79 45 302 609 18 1138 509 955
Cuts at: 7598 8030 8777 9045 1987
Size: 432 747 268 2715
Fragments arranged by size:
2715 1138 955 747 609 509 480 432
309 302 294 268 239 198 192 171
79 73 45 18
AfeI AGC'GCT
Cuts at: 854 854
Size: 9773
AflIII A'CryG_T
Cuts at: 501 634 767 4760 7125 8229 9206 501
Size: 133 133 3993 2365 1104 977 1068
Fragments arranged by size:
3993 2365 1104 1068 977 133 133
AgeI A'CCGG_T
Cuts at: 257 8161 257
Size: 7904 1869
AhdI GACnn_n'nnGTC
Cuts at: 5667 5667
Size: 9773
AleI CACnn'nnGTG
Cuts at: 1138 3151 1138
Size: 2013 7760
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1696 2053 2620 3711 1696
Size: 357 567 1091 7758
Fragments arranged by size:
7758 1091 567 357
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3028 3060 3028
Size: 32 9741
AlwNI CAG_nnn'CTG
Cuts at: 1776 2010 2077 2184 2202 4495 4596 5049 5950
Size: 234 67 107 18 2293 101 453 901
Cuts at: 5950 6274 6292 6381 6621 7078 8645 1776
Size: 324 18 89 240 457 1567 2904
Fragments arranged by size:
2904 2293 1567 901 457 453 324 240
234 107 101 89 67 18 18
ApaI G_GGCC'C
Cuts at: 3458 3504 4105 4755 5135 5264 5378 5476 6145
Size: 46 601 650 380 129 114 98 669
Cuts at: 6145 3458
Size: 7086
Fragments arranged by size:
7086 669 650 601 380 129 114 98
46
ApaLI G'TGCA_C
Cuts at: 2256 8543 2256
Size: 6287 3486
ApoI r'AATT_y
Cuts at: 3082 6777 9126 9382 3082
Size: 3695 2349 256 3473
Fragments arranged by size:
3695 3473 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 9773
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9773
AvaI C'yCGr_G
Cuts at: 3401 5263 5476 5868 6793 3401
Size: 1862 213 392 925 6381
Fragments arranged by size:
6381 1862 925 392 213
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3209 3242 3209
Size: 33 9740
BamHI G'GATC_C
Cuts at: 524 4865 6798 524
Size: 4341 1933 3499
Fragments arranged by size:
4341 3499 1933
BanI G'GyrC_C
Cuts at: 2174 2990 3225 3547 5937 6178 6789 7203 7451
Size: 816 235 322 2390 241 611 414 248
Cuts at: 7451 7486 2174
Size: 35 4461
Fragments arranged by size:
4461 2390 816 611 414 322 248 241
235 35
BanII G_rGCy'C
Cuts at: 293 974 2627 2841 3458 3504 3623 4105 4295
Size: 681 1653 214 617 46 119 482 190
Cuts at: 4295 4755 4820 5108 5135 5264 5365 5378 5387
Size: 460 65 288 27 129 101 13 9
Cuts at: 5387 5476 6145 6299 6388 6470 6787 7817 293
Size: 89 669 154 89 82 317 1030 2249
Fragments arranged by size:
2249 1653 1030 681 669 617 482 460
317 288 214 190 154 129 119 101
89 89 82 65 46 27 13 9
BbeI G_GCGC'C
Cuts at: 7207 7455 7207
Size: 248 9525
BbsI GAAGACnn'nnnn_
Cuts at: 2690 2903 5096 2690
Size: 213 2193 7367
Fragments arranged by size:
7367 2193 213
BbvCI CC'TCA_GC
Cuts at: 1696 4518 4610 1696
Size: 2822 92 6859
Fragments arranged by size:
6859 2822 92
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 291 325 3811 3845 291
Size: 34 3486 34 6219
Fragments arranged by size:
6219 3486 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4789 7671 8432 9773 4789
Size: 2882 761 1341 4789
Fragments arranged by size:
4789 2882 1341 761
BclI T'GATC_A
Cuts at: 153 7293 153
Size: 7140 2633
BfrBI ATG'CAT
Cuts at: 3345 3345
Size: 9773
BglI GCCn_nnn'nGGC
Cuts at: 403 1470 3883 5210 6099 6108 403
Size: 1067 2413 1327 889 9 4068
Fragments arranged by size:
4068 2413 1327 1067 889 9
BglII A'GATC_T
Cuts at: 4380 7288 9325 4380
Size: 2908 2037 4828
Fragments arranged by size:
4828 2908 2037
BlpI GC'TnA_GC
Cuts at: 1642 5098 5512 5635 6293 6885 1642
Size: 3456 414 123 658 592 4530
Fragments arranged by size:
4530 3456 658 592 414 123
Bme1580I G_kGCm'C
Cuts at: 2260 3458 3504 4105 4755 5135 5264 5378 5476
Size: 1198 46 601 650 380 129 114 98
Cuts at: 5476 5526 5942 6145 6181 6286 6435 7398 7491
Size: 50 416 203 36 105 149 963 93
Cuts at: 7491 8547 2260
Size: 1056 3486
Fragments arranged by size:
3486 1198 1056 963 650 601 416 380
203 149 129 114 105 98 93 50
46 36
BmgBI CAC'GTC
Cuts at: 979 1518 2631 3583 4761 979
Size: 539 1113 952 1178 5991
Fragments arranged by size:
5991 1178 1113 952 539
BmrI ACTGGGnnnn_n'
Cuts at: 5978 6639 6985 7066 7401 9545 5978
Size: 661 346 81 335 2144 6206
Fragments arranged by size:
6206 2144 661 346 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3021 4132 4579 5643 6389 8140 3021
Size: 1111 447 1064 746 1751 4654
Fragments arranged by size:
4654 1751 1111 1064 746 447
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 1555 1582 1604 1680 1744 1960 2026 2032
Size: 1110 27 22 76 64 216 66 6
Cuts at: 2032 2158 2188 2206 2308 2851 2944 3016 3245
Size: 126 30 18 102 543 93 72 229
Cuts at: 3245 3526 3910 4299 4729 5524 5677 5830 6712
Size: 281 384 389 430 795 153 153 882
Cuts at: 6712 6721 6748 445
Size: 9 27 3470
Fragments arranged by size:
3470 1110 882 795 543 430 389 384
281 229 216 153 153 126 102 93
76 72 66 64 30 27 27 22
18 9 6
Bpu10I CC'TnA_GC
Cuts at: 200 1696 3178 3617 3869 4518 4610 4666 5250
Size: 1496 1482 439 252 649 92 56 584
Cuts at: 5250 6329 6353 6382 6464 200
Size: 1079 24 29 82 3509
Fragments arranged by size:
3509 1496 1482 1079 649 584 439 252
92 82 56 29 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 1057 4921 6949 7828 8320 8618 682
Size: 375 3864 2028 879 492 298 1837
Fragments arranged by size:
3864 2028 1837 879 492 375 298
BsaI GGTCTCn'nnnn_
Cuts at: 1131 5761 6601 9477 1131
Size: 4630 840 2876 1427
Fragments arranged by size:
4630 2876 1427 840
BsaAI yAC'GTr
Cuts at: 7126 7126
Size: 9773
BsaBI GATnn'nnATC
Cuts at: 6 2485 7287 9324 6
Size: 2479 4802 2037 455
Fragments arranged by size:
4802 2479 2037 455
BsaHI Gr'CG_yC
Cuts at: 1057 2752 7204 7452 8154 9650 1057
Size: 1695 4452 248 702 1496 1180
Fragments arranged by size:
4452 1695 1496 1180 702 248
BsaWI w'CCGG_w
Cuts at: 257 328 521 731 2663 4736 6962 7483 8161
Size: 71 193 210 1932 2073 2226 521 678
Cuts at: 8161 8435 8582 8993 257
Size: 274 147 411 1037
Fragments arranged by size:
2226 2073 1932 1037 678 521 411 274
210 193 147 71
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1176 1206 3218 3248 4057 4087 4149 4179 5264
Size: 30 2012 30 809 30 62 30 1085
Cuts at: 5264 5294 6030 6060 1176
Size: 30 736 30 4889
Fragments arranged by size:
4889 2012 1085 809 736 62 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 280 730 1405 1677 1799 2155 2203 2464 2626
Size: 450 675 272 122 356 48 261 162
Cuts at: 2626 2932 3730 3733 3838 4030 4113 4116 4432
Size: 306 798 3 105 192 83 3 316
Cuts at: 4432 4627 5278 5521 5632 5812 6619 280
Size: 195 651 243 111 180 807 3434
Fragments arranged by size:
3434 807 798 675 651 450 356 316
306 272 261 243 195 192 180 162
122 111 105 83 48 3 3
BseYI C'CCAG_C
Cuts at: 398 410 982 1024 1098 1910 2321 2727 2968
Size: 12 572 42 74 812 411 406 241
Cuts at: 2968 3011 3741 4087 4488 4679 4949 5219 5550
Size: 43 730 346 401 191 270 270 331
Cuts at: 5550 5555 6124 6418 6434 6469 7198 7956 8533
Size: 5 569 294 16 35 729 758 577
Cuts at: 8533 9029 398
Size: 496 1142
Fragments arranged by size:
1142 812 758 730 729 577 572 569
496 411 406 401 346 331 294 270
270 241 191 74 43 42 35 16
12 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 815 913 1147 2013 2626 3646 3775 3802 3977
Size: 98 234 866 613 1020 129 27 175
Cuts at: 3977 5348 5996 815
Size: 1371 648 4592
Fragments arranged by size:
4592 1371 1020 866 648 613 234 175
129 98 27
BsiEI CG_ry'CG
Cuts at: 104 4227 4891 7315 7361 8569 104
Size: 4123 664 2424 46 1208 1308
Fragments arranged by size:
4123 2424 1308 1208 664 46
BsiHKAI G_wGCw'C
Cuts at: 293 1643 2260 3623 5087 5108 6388 6787 7565
Size: 1350 617 1363 1464 21 1280 399 778
Cuts at: 7565 7755 8547 293
Size: 190 792 1519
Fragments arranged by size:
1519 1464 1363 1350 1280 792 778 617
399 190 21
BsmI GAATG_Cn'
Cuts at: 3413 3413
Size: 9773
BsmBI CGTCTCn'nnnn_
Cuts at: 2637 2743 2637
Size: 106 9667
Bsp1286I G_dGCh'C
Cuts at: 293 974 1643 2260 2627 2841 3458 3504 3623
Size: 681 669 617 367 214 617 46 119
Cuts at: 3623 4105 4295 4755 4820 5087 5108 5135 5264
Size: 482 190 460 65 267 21 27 129
Cuts at: 5264 5365 5378 5387 5476 5526 5942 6145 6181
Size: 101 13 9 89 50 416 203 36
Cuts at: 6181 6286 6299 6388 6435 6470 6787 7398 7491
Size: 105 13 89 47 35 317 611 93
Cuts at: 7491 7565 7755 7817 8547 293
Size: 74 190 62 730 1519
Fragments arranged by size:
1519 730 681 669 617 617 611 482
460 416 367 317 267 214 203 190
190 129 119 105 101 93 89 89
74 65 62 50 47 46 36 35
27 21 13 13 9
BspEI T'CCGG_A
Cuts at: 521 731 8993 521
Size: 210 8262 1301
Fragments arranged by size:
8262 1301 210
BspHI T'CATG_A
Cuts at: 156 8949 156
Size: 8793 980
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3006 3824 5778 6049 6207 6310 6823 7037 7339
Size: 818 1954 271 158 103 513 214 302
Cuts at: 7339 7720 3006
Size: 381 5059
Fragments arranged by size:
5059 1954 818 513 381 302 271 214
158 103
BsrBI CCG'CTC
Cuts at: 50 2172 4919 5346 5430 5544 5625 8065 8119
Size: 2122 2747 427 84 114 81 2440 54
Cuts at: 8119 9603 9768 50
Size: 1484 165 55
Fragments arranged by size:
2747 2440 2122 1484 427 165 114 84
81 55 54
BsrDI GCAATG_nn'
Cuts at: 7685 7685
Size: 9773
BsrFI r'CCGG_y
Cuts at: 257 674 3641 4599 4806 4886 5297 5483 6264
Size: 417 2967 958 207 80 411 186 781
Cuts at: 6264 7771 7952 8161 257
Size: 1507 181 209 1869
Fragments arranged by size:
2967 1869 1507 958 781 417 411 209
207 186 181 80
BsrGI T'GTAC_A
Cuts at: 611 947 2957 611
Size: 336 2010 7427
Fragments arranged by size:
7427 2010 336
BssSI C'ACGA_G
Cuts at: 2794 3622 3820 5394 8044 8402 2794
Size: 828 198 1574 2650 358 4165
Fragments arranged by size:
4165 2650 1574 828 358 198
BstAPI GCAn_nnn'nTGC
Cuts at: 1518 4794 4803 6019 6274 7150 1518
Size: 3276 9 1216 255 876 4141
Fragments arranged by size:
4141 3276 1216 876 255 9
BstBI TT'CG_AA
Cuts at: 114 8134 114
Size: 8020 1753
BstEII G'GTnAC_C
Cuts at: 1879 6448 1879
Size: 4569 5204
BstXI CCAn_nnnn'nTGG
Cuts at: 1497 2064 2286 2994 4305 6260 1497
Size: 567 222 708 1311 1955 5010
Fragments arranged by size:
5010 1955 1311 708 567 222
BstYI r'GATC_y
Cuts at: 1 420 524 3021 3848 4380 4865 6798 7265
Size: 419 104 2497 827 532 485 1933 467
Cuts at: 7265 7288 7623 7869 8881 8967 8979 9325 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
2497 1933 1012 827 532 485 467 449
419 346 335 246 104 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 511 1388 5195 6194 511
Size: 877 3807 999 4090
Fragments arranged by size:
4090 3807 999 877
BtgI C'CryG_G
Cuts at: 434 1046 1589 3878 4783 4871 5309 5365 5387
Size: 612 543 2289 905 88 438 56 22
Cuts at: 5387 6202 434
Size: 815 4005
Fragments arranged by size:
4005 2289 905 815 612 543 438 88
56 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4629 7723 7904 8067 4629
Size: 3094 181 163 6335
Fragments arranged by size:
6335 3094 181 163
BtsI GCAGTG_nn'
Cuts at: 461 1159 1808 1869 2377 2520 2947 3601 4093
Size: 698 649 61 508 143 427 654 492
Cuts at: 4093 4645 6255 6429 7016 461
Size: 552 1610 174 587 3218
Fragments arranged by size:
3218 1610 698 654 649 587 552 508
492 427 174 143 61
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2535 2570 4152 4187 5124 5159 2535
Size: 35 1582 35 937 35 7149
Fragments arranged by size:
7149 1582 937 35 35 35
DraI TTT'AAA
Cuts at: 171 6772 171
Size: 6601 3172
DraIII CAC_nnn'GTG
Cuts at: 1310 2255 4538 1310
Size: 945 2283 6545
Fragments arranged by size:
6545 2283 945
DrdI GACnn_nn'nnGTC
Cuts at: 5044 7479 8337 5044
Size: 2435 858 6480
Fragments arranged by size:
6480 2435 858
EaeI y'GGCC_r
Cuts at: 941 2459 3122 3807 3875 4888 5202 5306 6100
Size: 1518 663 685 68 1013 314 104 794
Cuts at: 6100 6262 7358 7532 7923 7950 9295 941
Size: 162 1096 174 391 27 1345 1419
Fragments arranged by size:
1518 1419 1345 1096 1013 794 685 663
391 314 174 162 104 68 27
EagI C'GGCC_G
Cuts at: 4888 7358 4888
Size: 2470 7303
EarI CTCTTCn'nnn_
Cuts at: 1253 1316 2079 2640 2971 3130 3439 4086 4330
Size: 63 763 561 331 159 309 647 244
Cuts at: 4330 4546 4825 6149 7796 8006 1253
Size: 216 279 1324 1647 210 3020
Fragments arranged by size:
3020 1647 1324 763 647 561 331 309
279 244 216 210 159 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1645 2233 4645 4878 6513 7981 8291 8437 9583
Size: 588 2412 233 1635 1468 310 146 1146
Cuts at: 9583 1645
Size: 1835
Fragments arranged by size:
2412 1835 1635 1468 1146 588 310 233
146
EcoICRI GAG'CTC
Cuts at: 291 3621 5106 6386 6785 291
Size: 3330 1485 1280 399 3279
Fragments arranged by size:
3330 3279 1485 1280 399
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 1555 1582 1604 1680 1744 1960 1987 2026
Size: 1110 27 22 76 64 216 27 39
Cuts at: 2026 2032 2158 2179 2188 2206 2308 2377 2548
Size: 6 126 21 9 18 102 69 171
Cuts at: 2548 2621 2851 2915 2944 3016 3154 3245 3463
Size: 73 230 64 29 72 138 91 218
Cuts at: 3463 3526 3910 3943 4022 4067 4299 4369 4729
Size: 63 384 33 79 45 232 70 360
Cuts at: 4729 4978 4996 5524 5677 5830 6134 6643 6712
Size: 249 18 528 153 153 304 509 69
Cuts at: 6712 6721 6748 7598 8030 8777 9045 445
Size: 9 27 850 432 747 268 1173
Fragments arranged by size:
1173 1110 850 747 528 509 432 384
360 304 268 249 232 230 218 216
171 153 153 138 126 102 91 79
76 73 72 70 69 69 64 64
63 45 39 33 29 27 27 27
22 21 18 18 9 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1924 2578 3238 3820 4005 4975 1924
Size: 654 660 582 185 970 6722
Fragments arranged by size:
6722 970 660 654 582 185
EcoO109I rG'GnC_Cy
Cuts at: 234 1020 1865 2007 2496 2813 3501 3885 3963
Size: 786 845 142 489 317 688 384 78
Cuts at: 3963 3997 4102 4560 5046 5131 5211 5260 5374
Size: 34 105 458 486 85 80 49 114
Cuts at: 5374 5472 5473 6084 6414 6696 6912 234
Size: 98 1 611 330 282 216 3095
Fragments arranged by size:
3095 845 786 688 611 489 486 458
384 330 317 282 216 142 114 105
98 85 80 78 49 34 1
EcoRI G'AATT_C
Cuts at: 6777 6777
Size: 9773
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1992 7792 1992
Size: 5800 3973
FspI TGC'GCA
Cuts at: 7554 7554
Size: 9773
HaeII r_GCGC'y
Cuts at: 856 1193 1987 6293 7207 7455 8477 9456 856
Size: 337 794 4306 914 248 1022 979 1173
Fragments arranged by size:
4306 1173 1022 979 914 794 337 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1176 1208 2078 2110 3178 3210 4057 4063 4089
Size: 32 870 32 1068 32 847 6 26
Cuts at: 4089 4095 4252 4284 5653 5685 7615 7645 7647
Size: 6 157 32 1369 32 1930 30 2
Cuts at: 7647 7677 1176
Size: 30 3272
Fragments arranged by size:
3272 1930 1369 1068 870 847 157 32
32 32 32 32 30 30 26 6
6 2
HincII GTy'rAC
Cuts at: 480 1065 3972 6812 480
Size: 585 2907 2840 3441
Fragments arranged by size:
3441 2907 2840 585
HindIII A'AGCT_T
Cuts at: 165 656 1446 4034 165
Size: 491 790 2588 5904
Fragments arranged by size:
5904 2588 790 491
HpaI GTT'AAC
Cuts at: 1065 1065
Size: 9773
KasI G'GCGC_C
Cuts at: 7203 7451 7203
Size: 248 9525
KpnI G_GTAC'C
Cuts at: 2994 3229 6793 2994
Size: 235 3564 5974
Fragments arranged by size:
5974 3564 235
MfeI C'AATT_G
Cuts at: 3586 3586
Size: 9773
MluI A'CGCG_T
Cuts at: 9206 9206
Size: 9773
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 989 1160 3545 5669 5744 8444 8628 989
Size: 171 2385 2124 75 2700 184 2134
Fragments arranged by size:
2700 2385 2134 2124 184 171 75
MscI TGG'CCA
Cuts at: 943 2461 3124 3809 3877 5204 5308 7534 943
Size: 1518 663 685 68 1327 104 2226 3182
Fragments arranged by size:
3182 2226 1518 1327 685 663 104 68
MslI CAynn'nnrTG
Cuts at: 1051 1138 1495 2035 2062 2602 3151 7762 7889
Size: 87 357 540 27 540 549 4611 127
Cuts at: 7889 1051
Size: 2935
Fragments arranged by size:
4611 2935 549 540 540 357 127 87
27
MspA1I CmG'CkG
Cuts at: 402 414 799 1102 1658 1692 1764 1893 2199
Size: 12 385 303 556 34 72 129 306
Cuts at: 2199 2325 2731 3695 4087 4620 4953 4998 5058
Size: 126 406 964 392 533 333 45 60
Cuts at: 5058 5965 5979 6400 6884 7198 7558 8571 8816
Size: 907 14 421 484 314 360 1013 245
Cuts at: 8816 402
Size: 1359
Fragments arranged by size:
1359 1013 964 907 556 533 484 421
406 392 385 360 333 314 306 303
245 129 126 72 60 45 34 14
12
NaeI GCC'GGC
Cuts at: 3643 4601 4808 5485 6266 7954 3643
Size: 958 207 677 781 1688 5462
Fragments arranged by size:
5462 1688 958 781 677 207
NarI GG'CG_CC
Cuts at: 7204 7452 7204
Size: 248 9525
NcoI C'CATG_G
Cuts at: 434 1046 1589 3878 5309 5387 434
Size: 612 543 2289 1431 78 4820
Fragments arranged by size:
4820 2289 1431 612 543 78
NgoMIV G'CCGG_C
Cuts at: 3641 4599 4806 5483 6264 7952 3641
Size: 958 207 677 781 1688 5462
Fragments arranged by size:
5462 1688 958 781 677 207
NsiI A_TGCA'T
Cuts at: 3347 3347
Size: 9773
NspI r_CATG'y
Cuts at: 505 638 771 3345 3472 3657 3741 4806 5778
Size: 133 133 2574 127 185 84 1065 972
Cuts at: 5778 6504 6826 7326 8233 505
Size: 726 322 500 907 2045
Fragments arranged by size:
2574 2045 1065 972 907 726 500 322
185 133 133 127 84
PasI CC'CwG_GG
Cuts at: 358 1861 4567 4590 5128 6088 6279 358
Size: 1503 2706 23 538 960 191 3852
Fragments arranged by size:
3852 2706 1503 960 538 191 23
PciI A'CATG_T
Cuts at: 501 634 767 8229 501
Size: 133 133 7462 2045
Fragments arranged by size:
7462 2045 133 133
PflMI CCAn_nnn'nTGG
Cuts at: 1459 1875 2184 2418 3018 4538 5049 1459
Size: 416 309 234 600 1520 511 6183
Fragments arranged by size:
6183 1520 600 511 416 309 234
PfoI T'CCnGG_A
Cuts at: 1337 1559 4315 5654 6698 1337
Size: 222 2756 1339 1044 4412
Fragments arranged by size:
4412 2756 1339 1044 222
PmeI GTTT'AAAC
Cuts at: 6772 6772
Size: 9773
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8912 8944 8912
Size: 32 9741
PpuMI rG'GwC_Cy
Cuts at: 1020 1865 2813 3963 3997 4560 5046 6084 6696
Size: 845 948 1150 34 563 486 1038 612
Cuts at: 6696 1020
Size: 4097
Fragments arranged by size:
4097 1150 1038 948 845 612 563 486
34
PsiI TTA'TAA
Cuts at: 386 386
Size: 9773
PspOMI G'GGCC_C
Cuts at: 3454 3500 4101 4751 5131 5260 5374 5472 6141
Size: 46 601 650 380 129 114 98 669
Cuts at: 6141 3454
Size: 7086
Fragments arranged by size:
7086 669 650 601 380 129 114 98
46
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3262 3294 3262
Size: 32 9741
PstI C_TGCA'G
Cuts at: 248 1708 1803 1991 2339 2596 3993 4455 4482
Size: 1460 95 188 348 257 1397 462 27
Cuts at: 4482 4799 6307 6820 7505 248
Size: 317 1508 513 685 2516
Fragments arranged by size:
2516 1508 1460 1397 685 513 462 348
317 257 188 95 27
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9773
PvuII CAG'CTG
Cuts at: 402 799 1102 1658 1764 1893 2325 4087 4953
Size: 397 303 556 106 129 432 1762 866
Cuts at: 4953 5965 6400 7198 7558 402
Size: 1012 435 798 360 2617
Fragments arranged by size:
2617 1762 1012 866 798 556 435 432
397 360 303 129 106
RsrII CG'GwC_CG
Cuts at: 7968 7968
Size: 9773
SacI G_AGCT'C
Cuts at: 293 3623 5108 6388 6787 293
Size: 3330 1485 1280 399 3279
Fragments arranged by size:
3330 3279 1485 1280 399
SalI G'TCGA_C
Cuts at: 6810 6810
Size: 9773
SanDI GG'GwC_CC
Cuts at: 1865 1865
Size: 9773
SapI GCTCTTCn'nnn_
Cuts at: 4825 6149 7796 8006 4825
Size: 1324 1647 210 6592
Fragments arranged by size:
6592 1647 1324 210
SbfI CC_TGCA'GG
Cuts at: 6820 6820
Size: 9773
ScaI AGT'ACT
Cuts at: 2864 9290 2864
Size: 6426 3347
SfcI C'TryA_G
Cuts at: 33 244 1704 1799 1888 1987 2335 2592 3795
Size: 211 1460 95 89 99 348 257 1203
Cuts at: 3795 3989 4451 4478 4722 4795 6303 6816 7501
Size: 194 462 27 244 73 1508 513 685
Cuts at: 7501 8494 8685 33
Size: 993 191 1121
Fragments arranged by size:
1508 1460 1203 1121 993 685 513 462
348 257 244 211 194 191 99 95
89 73 27
SfiI GGCCn_nnn'nGGCC
Cuts at: 3883 5210 6099 6108 3883
Size: 1327 889 9 7548
Fragments arranged by size:
7548 1327 889 9
SfoI GGC'GCC
Cuts at: 7205 7453 7205
Size: 248 9525
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9773
SmaI CCC'GGG
Cuts at: 3403 6795 3403
Size: 3392 6381
SmlI C'TyrA_G
Cuts at: 697 1036 4936 6928 7843 8335 8597 697
Size: 339 3900 1992 915 492 262 1873
Fragments arranged by size:
3900 1992 1873 915 492 339 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9773
SphI G_CATG'C
Cuts at: 3472 3657 6826 7326 3472
Size: 185 3169 500 5919
Fragments arranged by size:
5919 3169 500 185
SrfI GCCC'GGGC
Cuts at: 3403 3403
Size: 9773
SspI AAT'ATT
Cuts at: 376 376
Size: 9773
StuI AGG'CCT
Cuts at: 826 4054 5866 6111 6327 826
Size: 3228 1812 245 216 4272
Fragments arranged by size:
4272 3228 1812 245 216
StyI C'CwwG_G
Cuts at: 434 827 987 1046 1589 2444 2618 2933 3323
Size: 393 160 59 543 855 174 315 390
Cuts at: 3323 3878 5135 5309 5387 6080 6410 6907 7261
Size: 555 1257 174 78 693 330 497 354
Cuts at: 7261 434
Size: 2946
Fragments arranged by size:
2946 1257 855 693 555 543 497 393
390 354 330 315 174 174 160 78
59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1189 1867 2445 3789 4044 4241 4545 6467 6579
Size: 678 578 1344 255 197 304 1922 112
Cuts at: 6579 7356 7489 7948 8157 1189
Size: 777 133 459 209 2805
Fragments arranged by size:
2805 1922 1344 777 678 578 459 304
255 209 197 133 112
TatI w'GTAC_w
Cuts at: 611 641 947 2862 2957 3896 4432 9288 611
Size: 30 306 1915 95 939 536 4856 1096
Fragments arranged by size:
4856 1915 1096 939 536 306 95 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4149 4181 5264 5296 6030 6062 9290 9322 4149
Size: 32 1083 32 734 32 3228 32 4600
Fragments arranged by size:
4600 3228 1083 734 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2092 3190 5971 7570 2092
Size: 1098 2781 1599 4295
Fragments arranged by size:
4295 2781 1599 1098
XbaI T'CTAG_A
Cuts at: 6804 6804
Size: 9773
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1101 3885 4084 4302 5385 5839 7268 1101
Size: 2784 199 218 1083 454 1429 3606
Fragments arranged by size:
3606 2784 1429 1083 454 218 199
XmaI C'CCGG_G
Cuts at: 3401 6793 3401
Size: 3392 6381
XmnI GAAnn'nnTTC
Cuts at: 3490 3490
Size: 9773
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AflII AscI AvrII BmtI BsiWI BssHII BstZ17I
ClaI EcoRV FseI FspAI NdeI NheI NotI NruI
PacI PmlI PshAI PspXI SacII SexAI SgrAI SnaBI
SwaI XhoI ZraI