Restriction map for
pF1KA0333
(Circular) (Six-base) MAPSORT of: pF1KA0333.seq Check: 2351 from: 1 to: 6680
pF1KA0333 6680 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1968 1968
Size: 6680
AatII G_ACGT'C
Cuts at: 2603 2603
Size: 6680
AccI GT'mk_AC
Cuts at: 3718 3718
Size: 6680
Acc65I G'GTAC_C
Cuts at: 2034 3696 2034
Size: 1662 5018
AclI AA'CG_TT
Cuts at: 6520 6520
Size: 6680
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 583 1916 2323 2398 2589 2852 3197 4505 4937
Size: 1333 407 75 191 263 345 1308 432
Cuts at: 4937 5684 5952 583
Size: 747 268 1311
Fragments arranged by size:
1333 1311 1308 747 432 407 345 268
263 191 75
AfeI AGC'GCT
Cuts at: 459 459
Size: 6680
AflIII A'CryG_T
Cuts at: 4032 5136 6113 4032
Size: 1104 977 4599
Fragments arranged by size:
4599 1104 977
AgeI A'CCGG_T
Cuts at: 5068 5068
Size: 6680
AhdI GACnn_n'nnGTC
Cuts at: 1713 1713
Size: 6680
AleI CACnn'nnGTG
Cuts at: 499 3040 3386 499
Size: 2541 346 3793
Fragments arranged by size:
3793 2541 346
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1087 1087
Size: 6680
AlwNI CAG_nnn'CTG
Cuts at: 276 339 2737 3985 5552 276
Size: 63 2398 1248 1567 1404
Fragments arranged by size:
2398 1567 1404 1248 63
ApaLI G'TGCA_C
Cuts at: 1504 5450 1504
Size: 3946 2734
ApoI r'AATT_y
Cuts at: 2106 2114 2669 3310 3684 6033 6289 2106
Size: 8 555 641 374 2349 256 2497
Fragments arranged by size:
2497 2349 641 555 374 256 8
AseI AT'TA_AT
Cuts at: 20 20
Size: 6680
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6680
AvaI C'yCGr_G
Cuts at: 126 763 1013 1306 1582 2920 3700 126
Size: 637 250 293 276 1338 780 3106
Fragments arranged by size:
3106 1338 780 637 293 276 250
BamHI G'GATC_C
Cuts at: 656 3705 656
Size: 3049 3631
BanI G'GyrC_C
Cuts at: 141 353 1147 1278 1285 1882 1956 2034 3129
Size: 212 794 131 7 597 74 78 1095
Cuts at: 3129 3696 4110 4358 4393 141
Size: 567 414 248 35 2428
Fragments arranged by size:
2428 1095 794 597 567 414 248 212
131 78 74 35 7
BanII G_rGCy'C
Cuts at: 404 635 715 764 2927 3458 3694 4724 404
Size: 231 80 49 2163 531 236 1030 2360
Fragments arranged by size:
2360 2163 1030 531 236 231 80 49
BbeI G_GCGC'C
Cuts at: 357 1282 4114 4362 357
Size: 925 2832 248 2675
Fragments arranged by size:
2832 2675 925 248
BbsI GAAGACnn'nnnn_
Cuts at: 1049 1199 1453 1712 1811 2485 2595 2756 2945
Size: 150 254 259 99 674 110 161 189
Cuts at: 2945 3412 3431 1049
Size: 467 19 4298
Fragments arranged by size:
4298 674 467 259 254 189 161 150
110 99 19
BciVI GTATCCnnnnn_n'
Cuts at: 1369 3338 4578 5339 6680 1369
Size: 1969 1240 761 1341 1369
Fragments arranged by size:
1969 1369 1341 1240 761
BclI T'GATC_A
Cuts at: 4200 4200
Size: 6680
BglI GCCn_nnn'nGGC
Cuts at: 1945 1954 1945
Size: 9 6671
BglII A'GATC_T
Cuts at: 1256 2434 4195 6232 1256
Size: 1178 1761 2037 1704
Fragments arranged by size:
2037 1761 1704 1178
BlpI GC'TnA_GC
Cuts at: 3149 3598 3792 3149
Size: 449 194 6037
Fragments arranged by size:
6037 449 194
Bme1580I G_kGCm'C
Cuts at: 1508 1885 3134 4305 4398 5454 1508
Size: 377 1249 1171 93 1056 2734
Fragments arranged by size:
2734 1249 1171 1056 377 93
BmgBI CAC'GTC
Cuts at: 407 3573 407
Size: 3166 3514
BmrI ACTGGGnnnn_n'
Cuts at: 477 743 1451 3892 3973 4308 6452 477
Size: 266 708 2441 81 335 2144 705
Fragments arranged by size:
2441 2144 708 705 335 266 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 453 1535 2370 5047 453
Size: 1082 835 2677 2086
Fragments arranged by size:
2677 2086 1082 835
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 406 881 1154 1505 1748 2533 2888 3215
Size: 135 475 273 351 243 785 355 327
Cuts at: 3215 271
Size: 3736
Fragments arranged by size:
3736 785 475 355 351 327 273 243
135
Bpu10I CC'TnA_GC
Cuts at: 836 1398 1478 836
Size: 562 80 6038
Fragments arranged by size:
6038 562 80
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1237 3418 3856 4735 5227 5525 1237
Size: 2181 438 879 492 298 2392
Fragments arranged by size:
2392 2181 879 492 438 298
BsaI GGTCTCn'nnnn_
Cuts at: 460 1122 1506 1528 6384 460
Size: 662 384 22 4856 756
Fragments arranged by size:
4856 756 662 384 22
BsaAI yAC'GTr
Cuts at: 1320 2414 2563 4033 1320
Size: 1094 149 1470 3967
Fragments arranged by size:
3967 1470 1094 149
BsaBI GATnn'nnATC
Cuts at: 6 2915 4194 6231 6
Size: 2909 1279 2037 455
Fragments arranged by size:
2909 2037 1279 455
BsaHI Gr'CG_yC
Cuts at: 354 425 740 1279 2070 2600 4111 4359 5061
Size: 71 315 539 791 530 1511 248 702
Cuts at: 5061 6557 354
Size: 1496 477
Fragments arranged by size:
1511 1496 791 702 539 530 477 315
248 71
BsaWI w'CCGG_w
Cuts at: 2793 3869 4390 5068 5342 5489 5900 2793
Size: 1076 521 678 274 147 411 3573
Fragments arranged by size:
3573 1076 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1653 1683 1746 1776 1653
Size: 30 63 30 6557
Fragments arranged by size:
6557 63 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 151 381 703 868 1735 3508 151
Size: 230 322 165 867 1773 3323
Fragments arranged by size:
3323 1773 867 322 230 165
BseYI C'CCAG_C
Cuts at: 276 793 2787 2955 4105 4863 5440 5936 276
Size: 517 1994 168 1150 758 577 496 1020
Fragments arranged by size:
1994 1150 1020 758 577 517 496 168
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1047 1099 1775 2075 2446 2762 1047
Size: 52 676 300 371 316 4965
Fragments arranged by size:
4965 676 371 316 300 52
BsiEI CG_ry'CG
Cuts at: 104 877 3003 4222 4268 5476 104
Size: 773 2126 1219 46 1208 1308
Fragments arranged by size:
2126 1308 1219 1208 773 46
BsiHKAI G_wGCw'C
Cuts at: 1479 1508 2584 3458 3694 4472 4662 5454 1479
Size: 29 1076 874 236 778 190 792 2705
Fragments arranged by size:
2705 1076 874 792 778 236 190 29
Bsp1286I G_dGCh'C
Cuts at: 404 635 715 764 1479 1508 1885 2584 2927
Size: 231 80 49 715 29 377 699 343
Cuts at: 2927 3134 3458 3694 4305 4398 4472 4662 4724
Size: 207 324 236 611 93 74 190 62
Cuts at: 4724 5454 404
Size: 730 1630
Fragments arranged by size:
1630 730 715 699 611 377 343 324
236 231 207 190 93 80 74 62
49 29
BspEI T'CCGG_A
Cuts at: 2793 5900 2793
Size: 3107 3573
BspHI T'CATG_A
Cuts at: 2186 5856 2186
Size: 3670 3010
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1474 1968 3730 3944 4246 4627 1474
Size: 494 1762 214 302 381 3527
Fragments arranged by size:
3527 1762 494 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2972 3019 4972 5026 6510 6675 50
Size: 2922 47 1953 54 1484 165 55
Fragments arranged by size:
2922 1953 1484 165 55 54 47
BsrDI GCAATG_nn'
Cuts at: 138 344 4592 138
Size: 206 4248 2226
Fragments arranged by size:
4248 2226 206
BsrFI r'CCGG_y
Cuts at: 1953 3656 4678 4859 5068 1953
Size: 1703 1022 181 209 3565
Fragments arranged by size:
3565 1703 1022 209 181
BsrGI T'GTAC_A
Cuts at: 1156 1156
Size: 6680
BssSI C'ACGA_G
Cuts at: 1804 4951 5309 1804
Size: 3147 358 3175
Fragments arranged by size:
3175 3147 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1975 3032 4057 1975
Size: 1057 1025 4598
Fragments arranged by size:
4598 1057 1025
BstBI TT'CG_AA
Cuts at: 195 5041 195
Size: 4846 1834
BstEII G'GTnAC_C
Cuts at: 2979 2979
Size: 6680
BstYI r'GATC_y
Cuts at: 1 656 1256 2434 3705 4172 4195 4530 4776
Size: 655 600 1178 1271 467 23 335 246
Cuts at: 4776 5788 5874 5886 6232 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1271 1178 1012 655 600 467 449 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 146 1869 146
Size: 1723 4957
BtgI C'CryG_G
Cuts at: 107 357 1736 1772 1900 3041 3534 107
Size: 250 1379 36 128 1141 493 3253
Fragments arranged by size:
3253 1379 1141 493 250 128 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 590 2081 2189 4630 4811 4974 590
Size: 1491 108 2441 181 163 2296
Fragments arranged by size:
2441 2296 1491 181 163 108
BtsI GCAGTG_nn'
Cuts at: 303 1559 3923 303
Size: 1256 2364 3060
Fragments arranged by size:
3060 2364 1256
DraI TTT'AAA
Cuts at: 3679 3679
Size: 6680
DraIII CAC_nnn'GTG
Cuts at: 3081 3081
Size: 6680
DrdI GACnn_nn'nnGTC
Cuts at: 4386 5244 4386
Size: 858 5822
EaeI y'GGCC_r
Cuts at: 2913 3000 3537 4265 4439 4830 4857 6202 2913
Size: 87 537 728 174 391 27 1345 3391
Fragments arranged by size:
3391 1345 728 537 391 174 87 27
EagI C'GGCC_G
Cuts at: 3000 4265 3000
Size: 1265 5415
EarI CTCTTCn'nnn_
Cuts at: 525 1503 2332 2572 2656 3485 4703 4913 525
Size: 978 829 240 84 829 1218 210 2292
Fragments arranged by size:
2292 1218 978 829 829 240 210 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2513 4888 5198 5344 6490 2513
Size: 2375 310 146 1146 2703
Fragments arranged by size:
2703 2375 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3456 3692 3456
Size: 236 6444
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 406 583 881 1154 1505 1748 1916 2323
Size: 135 177 298 273 351 243 168 407
Cuts at: 2323 2398 2533 2589 2852 2888 3197 3215 4505
Size: 75 135 56 263 36 309 18 1290
Cuts at: 4505 4937 5684 5952 271
Size: 432 747 268 999
Fragments arranged by size:
1290 999 747 432 407 351 309 298
273 268 263 243 177 168 135 135
75 56 36 18
EcoNI CCTnn'n_nnAGG
Cuts at: 3358 3358
Size: 6680
EcoO109I rG'GnC_Cy
Cuts at: 644 694 2307 3006 3579 3609 3819 644
Size: 50 1613 699 573 30 210 3505
Fragments arranged by size:
3505 1613 699 573 210 50 30
EcoRI G'AATT_C
Cuts at: 3684 3684
Size: 6680
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3129 4699 3129
Size: 1570 5110
FspI TGC'GCA
Cuts at: 4461 4461
Size: 6680
HaeII r_GCGC'y
Cuts at: 357 461 684 1282 2590 3605 3657 4114 4362
Size: 104 223 598 1308 1015 52 457 248
Cuts at: 4362 5384 6363 357
Size: 1022 979 674
Fragments arranged by size:
1308 1022 1015 979 674 598 457 248
223 104 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 391 423 544 576 648 680 1168 1200 1653
Size: 32 121 32 72 32 488 32 453
Cuts at: 1653 1685 1699 1731 2265 2297 2743 2775 4522
Size: 32 14 32 534 32 446 32 1747
Cuts at: 4522 4552 4554 4584 391
Size: 30 2 30 2487
Fragments arranged by size:
2487 1747 534 488 453 446 121 72
32 32 32 32 32 32 32 32
30 30 14 2
HincII GTy'rAC
Cuts at: 3719 3719
Size: 6680
KasI G'GCGC_C
Cuts at: 353 1278 4110 4358 353
Size: 925 2832 248 2675
Fragments arranged by size:
2832 2675 925 248
KpnI G_GTAC'C
Cuts at: 2038 3700 2038
Size: 1662 5018
MluI A'CGCG_T
Cuts at: 6113 6113
Size: 6680
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 800 2513 5351 5535 800
Size: 1713 2838 184 1945
Fragments arranged by size:
2838 1945 1713 184
MscI TGG'CCA
Cuts at: 2915 4441 2915
Size: 1526 5154
MslI CAynn'nnrTG
Cuts at: 499 2873 3040 3076 3386 4669 4796 499
Size: 2374 167 36 310 1283 127 2383
Fragments arranged by size:
2383 2374 1283 310 167 127 36
MspA1I CmG'CkG
Cuts at: 276 359 499 968 1774 1936 1966 2002 2803
Size: 83 140 469 806 162 30 36 801
Cuts at: 2803 2934 3597 3791 4105 4465 5478 5723 276
Size: 131 663 194 314 360 1013 245 1233
Fragments arranged by size:
1233 1013 806 801 663 469 360 314
245 194 162 140 131 83 36 30
NaeI GCC'GGC
Cuts at: 3658 4861 3658
Size: 1203 5477
NarI GG'CG_CC
Cuts at: 354 1279 4111 4359 354
Size: 925 2832 248 2675
Fragments arranged by size:
2832 2675 925 248
NcoI C'CATG_G
Cuts at: 107 1736 1900 107
Size: 1629 164 4887
Fragments arranged by size:
4887 1629 164
NgoMIV G'CCGG_C
Cuts at: 3656 4859 3656
Size: 1203 5477
NspI r_CATG'y
Cuts at: 375 2616 3297 3733 4233 5140 375
Size: 2241 681 436 500 907 1915
Fragments arranged by size:
2241 1915 907 681 500 436
PasI CC'CwG_GG
Cuts at: 2896 2896
Size: 6680
PciI A'CATG_T
Cuts at: 5136 5136
Size: 6680
PflMI CCAn_nnn'nTGG
Cuts at: 1924 2911 3081 1924
Size: 987 170 5523
Fragments arranged by size:
5523 987 170
PfoI T'CCnGG_A
Cuts at: 2904 2904
Size: 6680
PmeI GTTT'AAAC
Cuts at: 3679 3679
Size: 6680
PmlI CAC'GTG
Cuts at: 1320 2414 2563 1320
Size: 1094 149 5437
Fragments arranged by size:
5437 1094 149
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5819 5851 5819
Size: 32 6648
PpuMI rG'GwC_Cy
Cuts at: 694 2307 3006 3579 3609 694
Size: 1613 699 573 30 3765
Fragments arranged by size:
3765 1613 699 573 30
PspXI vC'TCGA_Gb
Cuts at: 1013 1013
Size: 6680
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2027 2059 2027
Size: 32 6648
PstI C_TGCA'G
Cuts at: 1348 1965 3727 4412 1348
Size: 617 1762 685 3616
Fragments arranged by size:
3616 1762 685 617
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6680
PvuII CAG'CTG
Cuts at: 276 499 1936 2803 4105 4465 276
Size: 223 1437 867 1302 360 2491
Fragments arranged by size:
2491 1437 1302 867 360 223
RsrII CG'GwC_CG
Cuts at: 1951 4875 1951
Size: 2924 3756
SacI G_AGCT'C
Cuts at: 3458 3694 3458
Size: 236 6444
SacII CC_GC'GG
Cuts at: 360 1775 360
Size: 1415 5265
SalI G'TCGA_C
Cuts at: 3717 3717
Size: 6680
SanDI GG'GwC_CC
Cuts at: 694 3006 694
Size: 2312 4368
SapI GCTCTTCn'nnn_
Cuts at: 2572 4703 4913 2572
Size: 2131 210 4339
Fragments arranged by size:
4339 2131 210
SbfI CC_TGCA'GG
Cuts at: 3727 3727
Size: 6680
ScaI AGT'ACT
Cuts at: 6197 6197
Size: 6680
SexAI A'CCwGG_T
Cuts at: 3645 3645
Size: 6680
SfcI C'TryA_G
Cuts at: 33 1344 1961 3723 4408 5401 5592 33
Size: 1311 617 1762 685 993 191 1121
Fragments arranged by size:
1762 1311 1121 993 685 617 191
SfoI GGC'GCC
Cuts at: 355 1280 4112 4360 355
Size: 925 2832 248 2675
Fragments arranged by size:
2832 2675 925 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6680
SmaI CCC'GGG
Cuts at: 1308 3702 1308
Size: 2394 4286
SmlI C'TyrA_G
Cuts at: 1013 1252 3433 3835 4750 5242 5504 1013
Size: 239 2181 402 915 492 262 2189
Fragments arranged by size:
2189 2181 915 492 402 262 239
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6680
SphI G_CATG'C
Cuts at: 375 3733 4233 375
Size: 3358 500 2822
Fragments arranged by size:
3358 2822 500
StuI AGG'CCT
Cuts at: 2852 2852
Size: 6680
StyI C'CwwG_G
Cuts at: 107 1197 1736 1900 2073 3814 4168 107
Size: 1090 539 164 173 1741 354 2619
Fragments arranged by size:
2619 1741 1090 539 354 173 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 891 3088 3329 3656 4263 4396 4855 5064 891
Size: 2197 241 327 607 133 459 209 2507
Fragments arranged by size:
2507 2197 607 459 327 241 209 133
TatI w'GTAC_w
Cuts at: 1156 1340 6195 1156
Size: 184 4855 1641
Fragments arranged by size:
4855 1641 184
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2552 2584 6197 6229 2552
Size: 32 3613 32 3003
Fragments arranged by size:
3613 3003 32 32
Tth111I GACn'n_nGTC
Cuts at: 4477 4477
Size: 6680
XbaI T'CTAG_A
Cuts at: 3711 3711
Size: 6680
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 729 2508 4175 729
Size: 1779 1667 3234
Fragments arranged by size:
3234 1779 1667
XhoI C'TCGA_G
Cuts at: 1013 1013
Size: 6680
XmaI C'CCGG_G
Cuts at: 1306 3700 1306
Size: 2394 4286
XmnI GAAnn'nnTTC
Cuts at: 980 980
Size: 6680
ZraI GAC'GTC
Cuts at: 2601 2601
Size: 6680
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AloI ApaI AscI AvrII BaeI BbvCI BcgI
BfrBI BmtI BsiWI BsmI BsmBI BssHII BstXI BstZ17I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PshAI PsiI
PspOMI SfiI SgrAI SnaBI SrfI SspI SwaI